Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20312.t1 | XP_003611549.2 | 89 | 118 | 11 | 1 | 1 | 118 | 1 | 116 | 3.30E-47 | 197.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20312.t1 | G7K2F6 | 89.0 | 118 | 11 | 1 | 1 | 118 | 1 | 116 | 2.3e-47 | 197.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055961 | MS.gene20312 | 0.807143 | 5.84E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20312.t1 | MTR_5g015190 | 87.500 | 104 | 11 | 1 | 1 | 104 | 1 | 102 | 5.39e-59 | 177 |
| MS.gene20312.t1 | MTR_4g097350 | 33.553 | 152 | 54 | 5 | 9 | 115 | 32 | 181 | 3.83e-14 | 65.5 |
| MS.gene20312.t1 | MTR_4g097280 | 53.226 | 62 | 28 | 1 | 9 | 69 | 31 | 92 | 3.02e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20312.t1 | AT2G22880 | 37.607 | 117 | 59 | 4 | 1 | 117 | 1 | 103 | 9.24e-12 | 57.8 |
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACCCTAACTGCTGTTAA+AGG | 0.195754 | 5.1:+7817082 | None:intergenic |
| GACAAAGCAATCAGGTTTAT+TGG | 0.268685 | 5.1:-7816912 | MS.gene20312:CDS |
| TGTTGTTGAGGCTTGAATTC+TGG | 0.279950 | 5.1:+7817007 | None:intergenic |
| GCACCTGTTGTTAGTGATAA+AGG | 0.285706 | 5.1:-7816962 | MS.gene20312:CDS |
| CACCTGTTGTTAGTGATAAA+GGG | 0.389424 | 5.1:-7816961 | MS.gene20312:CDS |
| CACAACCTTTAACAGCAGTT+AGG | 0.390436 | 5.1:-7817087 | MS.gene20312:CDS |
| CTTCACCATCTTCTCAGAGT+TGG | 0.407398 | 5.1:-7816877 | MS.gene20312:CDS |
| CACCATCTTCTCAGAGTTGG+TGG | 0.431356 | 5.1:-7816874 | MS.gene20312:CDS |
| ATTATGAGACTTAGGATAAG+AGG | 0.439722 | 5.1:+7817174 | None:intergenic |
| AATGAATTATTATGAGACTT+AGG | 0.448016 | 5.1:+7817166 | None:intergenic |
| ACAACCTTTAACAGCAGTTA+GGG | 0.450601 | 5.1:-7817086 | MS.gene20312:CDS |
| ACCCCTTTATCACTAACAAC+AGG | 0.485193 | 5.1:+7816959 | None:intergenic |
| AGTGGTGTGACAAAGCAATC+AGG | 0.507552 | 5.1:-7816920 | MS.gene20312:CDS |
| GACCACCAACTCTGAGAAGA+TGG | 0.520594 | 5.1:+7816872 | None:intergenic |
| ATTGTGGCTTTCTAACGGAA+TGG | 0.529161 | 5.1:+7817140 | None:intergenic |
| AGACTTAGGATAAGAGGTGG+TGG | 0.557065 | 5.1:+7817180 | None:intergenic |
| GGTGGTGGAATAAGCTTCCA+TGG | 0.561828 | 5.1:+7817195 | None:intergenic |
| ACAAATTGTTGATGTTGTTG+AGG | 0.565750 | 5.1:+7816995 | None:intergenic |
| GAAAGCCACAATCAAAACCA+TGG | 0.621885 | 5.1:-7817129 | MS.gene20312:CDS |
| GAGTTAGTTCAACAACTCAC+AGG | 0.631511 | 5.1:-7817034 | MS.gene20312:CDS |
| ACCTGTTGTTAGTGATAAAG+GGG | 0.645367 | 5.1:-7816960 | MS.gene20312:CDS |
| GTGCAATATCAAGAGAGTAG+TGG | 0.659499 | 5.1:-7816938 | MS.gene20312:CDS |
| ATGAGACTTAGGATAAGAGG+TGG | 0.667730 | 5.1:+7817177 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAATTATTATGAGACTT+AGG | + | chr5.1:7816889-7816908 | None:intergenic | 20.0% |
| ACAAATTGTTGATGTTGTTG+AGG | + | chr5.1:7817060-7817079 | None:intergenic | 30.0% | |
| ATTATGAGACTTAGGATAAG+AGG | + | chr5.1:7816881-7816900 | None:intergenic | 30.0% | |
| !! | TTTTGATTGTGGCTTTCTAA+CGG | + | chr5.1:7816920-7816939 | None:intergenic | 30.0% |
| !!! | TTTTTCCATGGTTTTGATTG+TGG | + | chr5.1:7816931-7816950 | None:intergenic | 30.0% |
| ACAACCTTTAACAGCAGTTA+GGG | - | chr5.1:7816966-7816985 | MS.gene20312:CDS | 35.0% | |
| ACCTGTTGTTAGTGATAAAG+GGG | - | chr5.1:7817092-7817111 | MS.gene20312:CDS | 35.0% | |
| CACCTGTTGTTAGTGATAAA+GGG | - | chr5.1:7817091-7817110 | MS.gene20312:CDS | 35.0% | |
| GACAAAGCAATCAGGTTTAT+TGG | - | chr5.1:7817140-7817159 | MS.gene20312:CDS | 35.0% | |
| TAAACCCTAACTGCTGTTAA+AGG | + | chr5.1:7816973-7816992 | None:intergenic | 35.0% | |
| ACCATGGAAAAAAGCTTCAG+TGG | - | chr5.1:7816939-7816958 | MS.gene20312:CDS | 40.0% | |
| ACCCCTTTATCACTAACAAC+AGG | + | chr5.1:7817096-7817115 | None:intergenic | 40.0% | |
| ATGAGACTTAGGATAAGAGG+TGG | + | chr5.1:7816878-7816897 | None:intergenic | 40.0% | |
| ATTGTGGCTTTCTAACGGAA+TGG | + | chr5.1:7816915-7816934 | None:intergenic | 40.0% | |
| CACAACCTTTAACAGCAGTT+AGG | - | chr5.1:7816965-7816984 | MS.gene20312:CDS | 40.0% | |
| GAAAGCCACAATCAAAACCA+TGG | - | chr5.1:7816923-7816942 | MS.gene20312:CDS | 40.0% | |
| GAGTTAGTTCAACAACTCAC+AGG | - | chr5.1:7817018-7817037 | MS.gene20312:CDS | 40.0% | |
| GCACCTGTTGTTAGTGATAA+AGG | - | chr5.1:7817090-7817109 | MS.gene20312:CDS | 40.0% | |
| GTGCAATATCAAGAGAGTAG+TGG | - | chr5.1:7817114-7817133 | MS.gene20312:CDS | 40.0% | |
| TGTTGTTGAGGCTTGAATTC+TGG | + | chr5.1:7817048-7817067 | None:intergenic | 40.0% | |
| AGACTTAGGATAAGAGGTGG+TGG | + | chr5.1:7816875-7816894 | None:intergenic | 45.0% | |
| AGTGGTGTGACAAAGCAATC+AGG | - | chr5.1:7817132-7817151 | MS.gene20312:CDS | 45.0% | |
| CTTCACCATCTTCTCAGAGT+TGG | - | chr5.1:7817175-7817194 | MS.gene20312:CDS | 45.0% | |
| !!! | GCCACTGAAGCTTTTTTCCA+TGG | + | chr5.1:7816943-7816962 | None:intergenic | 45.0% |
| CACCATCTTCTCAGAGTTGG+TGG | - | chr5.1:7817178-7817197 | MS.gene20312:CDS | 50.0% | |
| ! | GACCACCAACTCTGAGAAGA+TGG | + | chr5.1:7817183-7817202 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 7816859 | 7817215 | 7816859 | ID=MS.gene20312 |
| chr5.1 | mRNA | 7816859 | 7817215 | 7816859 | ID=MS.gene20312.t1;Parent=MS.gene20312 |
| chr5.1 | exon | 7816859 | 7817215 | 7816859 | ID=MS.gene20312.t1.exon1;Parent=MS.gene20312.t1 |
| chr5.1 | CDS | 7816859 | 7817215 | 7816859 | ID=cds.MS.gene20312.t1;Parent=MS.gene20312.t1 |
| Gene Sequence |
| Protein sequence |
