AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055961


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055961.t1 MTR_7g093820 98.718 156 2 0 1 156 39 194 1.62e-114 322
MS.gene055961.t1 MTR_7g093850 97.436 156 4 0 1 156 39 194 4.45e-113 318
MS.gene055961.t1 MTR_7g093830 96.795 156 5 0 1 156 38 193 6.75e-112 315
MS.gene055961.t1 MTR_7g093790 79.487 156 32 0 1 156 36 191 6.12e-94 270
MS.gene055961.t1 MTR_7g093870 59.375 160 59 3 2 156 35 193 9.28e-65 197
MS.gene055961.t1 MTR_4g013350 52.229 157 73 2 1 156 42 197 9.16e-57 176
MS.gene055961.t1 MTR_4g013345 53.503 157 71 2 1 156 41 196 1.87e-56 175
MS.gene055961.t1 MTR_4g013335 51.592 157 72 3 1 156 43 196 9.91e-55 171
MS.gene055961.t1 MTR_1g054525 54.605 152 66 2 5 156 44 192 3.78e-54 169
MS.gene055961.t1 MTR_4g013325 54.110 146 66 1 11 156 55 199 1.11e-53 168
MS.gene055961.t1 MTR_4g013320 51.316 152 73 1 5 156 49 199 1.31e-53 168
MS.gene055961.t1 MTR_4g013330 50.314 159 75 3 1 156 42 199 3.23e-53 167
MS.gene055961.t1 MTR_4g073950 52.941 153 70 2 5 156 41 192 2.14e-51 162
MS.gene055961.t1 MTR_4g013310 50.658 152 72 2 5 156 49 197 9.68e-50 158
MS.gene055961.t1 MTR_3g105640 51.634 153 71 3 6 156 37 188 3.02e-49 157
MS.gene055961.t1 MTR_3g105630 49.351 154 74 3 6 156 37 189 1.12e-48 155
MS.gene055961.t1 MTR_7g070390 51.316 152 72 2 5 156 43 192 7.72e-47 150
MS.gene055961.t1 MTR_4g013385 46.711 152 79 2 5 156 41 190 4.30e-45 146
MS.gene055961.t1 MTR_0433s0040 46.711 152 79 2 5 156 41 190 4.30e-45 146
MS.gene055961.t1 MTR_5g096120 49.032 155 72 4 7 156 37 189 6.80e-44 143
MS.gene055961.t1 MTR_1g056370 48.299 147 73 3 10 155 41 185 1.21e-42 140
MS.gene055961.t1 MTR_4g013770 43.243 148 82 2 10 156 45 191 7.38e-42 138
MS.gene055961.t1 MTR_3g034030 46.358 151 76 4 7 156 39 185 5.78e-40 133
MS.gene055961.t1 MTR_4g078885 45.395 152 78 4 6 156 38 185 2.37e-39 131
MS.gene055961.t1 MTR_4g013355 40.789 152 79 1 5 156 39 179 1.35e-36 124
MS.gene055961.t1 MTR_4g122110 40.397 151 89 1 6 156 31 180 5.15e-30 107
MS.gene055961.t1 MTR_4g122130 40.397 151 89 1 6 156 31 180 9.74e-30 107
MS.gene055961.t1 MTR_4g049570 32.215 149 100 1 3 151 26 173 2.95e-21 85.1
MS.gene055961.t1 MTR_4g049550 30.822 146 100 1 6 151 29 173 1.69e-20 83.2
MS.gene055961.t1 MTR_1g046490 40.000 95 55 2 40 134 119 211 3.46e-14 67.8
MS.gene055961.t1 MTR_1g046800 41.053 95 54 2 40 134 133 225 3.51e-14 67.8
MS.gene055961.t1 MTR_1g046500 41.053 95 54 2 40 134 133 225 4.87e-14 67.4
MS.gene055961.t1 MTR_1g115510 36.154 130 75 5 33 155 225 353 4.42e-13 65.9
MS.gene055961.t1 MTR_4g062520 37.383 107 66 1 33 139 177 282 1.73e-12 63.9
MS.gene055961.t1 MTR_4g062470 37.383 107 66 1 33 139 178 283 2.23e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055961.t1 AT5G42500 52.941 153 69 2 3 155 36 185 1.89e-53 167
MS.gene055961.t1 AT1G65870 52.288 153 72 1 3 155 38 189 2.54e-51 162
MS.gene055961.t1 AT5G42510 51.634 153 71 2 3 155 33 182 2.91e-51 162
MS.gene055961.t1 AT1G22900 51.634 153 71 2 3 155 44 193 1.77e-49 157
MS.gene055961.t1 AT5G49040 46.711 152 80 1 5 156 41 191 6.84e-48 153
MS.gene055961.t1 AT1G58170 45.806 155 83 1 2 156 32 185 7.93e-48 153
MS.gene055961.t1 AT1G55210 47.134 157 78 3 1 156 35 187 9.19e-48 153
MS.gene055961.t1 AT1G55210 47.134 157 78 3 1 156 35 187 9.19e-48 153
MS.gene055961.t1 AT3G13650 46.104 154 79 2 3 156 37 186 1.27e-46 150
MS.gene055961.t1 AT3G13662 49.286 140 67 2 4 143 37 172 1.07e-43 142
MS.gene055961.t1 AT2G21100 48.000 150 77 1 6 155 39 187 3.71e-43 141
MS.gene055961.t1 AT2G21100 48.000 150 77 1 6 155 39 187 3.71e-43 141
MS.gene055961.t1 AT2G21110 43.709 151 80 2 10 156 37 186 9.21e-42 138
MS.gene055961.t1 AT4G38700 44.304 158 83 3 4 156 33 190 6.22e-40 133
MS.gene055961.t1 AT5G49030 48.462 130 66 1 5 134 41 169 4.08e-38 137
MS.gene055961.t1 AT3G58090 45.522 134 72 1 22 155 139 271 4.36e-34 120
MS.gene055961.t1 AT3G13660 45.378 119 64 1 38 156 8 125 5.65e-34 116
MS.gene055961.t1 AT4G23690 34.586 133 75 5 5 131 36 162 1.71e-13 65.1
MS.gene055961.t1 AT3G24020 36.842 95 58 2 40 134 126 218 2.69e-11 60.1
MS.gene055961.t1 AT4G13580 35.789 95 59 2 40 134 127 219 6.58e-11 58.9

Find 45 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAAAACACCTAACTAATAA+TGG 0.327962 7.4:-15235771 None:intergenic
TGGAAGTACACTTAGCATAT+TGG 0.334562 7.4:+15235594 MS.gene055961:CDS
ACAATGGCCATTTCTCTAAC+AGG 0.363166 7.4:-15235640 None:intergenic
ACAAATACCATGCTAGGTTT+TGG 0.370876 7.4:-15235376 None:intergenic
GGAAGTACACTTAGCATATT+GGG 0.380807 7.4:+15235595 MS.gene055961:CDS
AGCCTATCCCCAACAATGTT+GGG 0.408783 7.4:+15235295 None:intergenic
ATGGCCATTGTTGGTGGGAC+TGG 0.413384 7.4:+15235652 MS.gene055961:CDS
TACTTTCACGACATTGTGTC+AGG 0.427188 7.4:+15235352 MS.gene055961:CDS
CGTTCTGGCCCAGCCGTCAA+TGG 0.431463 7.4:-15235463 None:intergenic
ATGGACGATCCATTGACGGC+TGG 0.438919 7.4:+15235454 MS.gene055961:CDS
TATTCCGTTGATTACACTAA+AGG 0.444889 7.4:+15235715 MS.gene055961:CDS
GAGTGAACCTGTTAGAGAAA+TGG 0.463422 7.4:+15235633 MS.gene055961:CDS
TTCACTGATGGAGATTTCAA+TGG 0.465136 7.4:+15235574 MS.gene055961:CDS
ACAAGTTTCGAGTCACGTTC+TGG 0.465414 7.4:-15235478 None:intergenic
TTCTATATCACAAGAGGAGA+TGG 0.477985 7.4:+15235525 MS.gene055961:CDS
ATTCGGGACGGTTGTGGCGA+TGG 0.478959 7.4:+15235435 MS.gene055961:CDS
CGTGAAAGTAAAAGTGAAGA+TGG 0.487615 7.4:-15235339 None:intergenic
GTTGCCATTCGGGACGGTTG+TGG 0.502902 7.4:+15235429 MS.gene055961:CDS
TGGACGATCCATTGACGGCT+GGG 0.512893 7.4:+15235455 MS.gene055961:CDS
AGAGGAGATGGGACTAATGA+TGG 0.528699 7.4:+15235537 MS.gene055961:CDS
TGACTCGAAACTTGTGGGTA+AGG 0.534298 7.4:+15235486 MS.gene055961:CDS
GAACGTGACTCGAAACTTGT+GGG 0.536601 7.4:+15235481 MS.gene055961:CDS
GGCGATGGACGATCCATTGA+CGG 0.538554 7.4:+15235450 MS.gene055961:CDS
GTTAGAGAAATGGCCATTGT+TGG 0.545345 7.4:+15235643 MS.gene055961:CDS
AAACTTGTGGGTAAGGCTCA+AGG 0.545821 7.4:+15235493 MS.gene055961:CDS
TCAGCTACAAATACCATGCT+AGG 0.557255 7.4:-15235382 None:intergenic
TTACACTTCTATATCACAAG+AGG 0.558364 7.4:+15235519 MS.gene055961:CDS
AAATGCGTTGCCATTCGGGA+CGG 0.561926 7.4:+15235423 MS.gene055961:CDS
ATGACCATGGCATTCACTGA+TGG 0.562304 7.4:+15235562 MS.gene055961:CDS
AGAGAAATGGCCATTGTTGG+TGG 0.569066 7.4:+15235646 MS.gene055961:CDS
AGCTCCAGTCCCACCAACAA+TGG 0.574074 7.4:-15235656 None:intergenic
CACTAAAGGAGATGCCATTG+TGG 0.590470 7.4:+15235729 MS.gene055961:CDS
ATCGCCACAACCGTCCCGAA+TGG 0.592145 7.4:-15235433 None:intergenic
TCTATATCACAAGAGGAGAT+GGG 0.592301 7.4:+15235526 MS.gene055961:CDS
AGAACGTGACTCGAAACTTG+TGG 0.594960 7.4:+15235480 MS.gene055961:CDS
ATCTCCTTTAGTGTAATCAA+CGG 0.597699 7.4:-15235719 None:intergenic
GAGAAATGGCCATTGTTGGT+GGG 0.599344 7.4:+15235647 MS.gene055961:CDS
GTGAAAGTAAAAGTGAAGAT+GGG 0.616560 7.4:-15235338 None:intergenic
GTCAGGACCAAAACCTAGCA+TGG 0.622028 7.4:+15235369 MS.gene055961:CDS
TGTAGCTGAGCCCAATGGCA+AGG 0.624333 7.4:+15235396 MS.gene055961:CDS
ATCTCCATCAGTGAATGCCA+TGG 0.633818 7.4:-15235566 None:intergenic
TTTCGTACGTGGGTATGCTG+AGG 0.640443 7.4:+15235684 MS.gene055961:CDS
GTATTTGTAGCTGAGCCCAA+TGG 0.650930 7.4:+15235391 MS.gene055961:CDS
AAATATGTCATATTCCACAA+TGG 0.686079 7.4:-15235743 None:intergenic
ACTAATGATGGTGATGACCA+TGG 0.702330 7.4:+15235549 MS.gene055961:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATATGTCATATTCCACAA+TGG - chr7.4:15235746-15235765 None:intergenic 25.0%
ATCTCCTTTAGTGTAATCAA+CGG - chr7.4:15235722-15235741 None:intergenic 30.0%
GTGAAAGTAAAAGTGAAGAT+GGG - chr7.4:15235341-15235360 None:intergenic 30.0%
TTACACTTCTATATCACAAG+AGG + chr7.4:15235519-15235538 MS.gene055961:CDS 30.0%
! TATTCCGTTGATTACACTAA+AGG + chr7.4:15235715-15235734 MS.gene055961:CDS 30.0%
CGTGAAAGTAAAAGTGAAGA+TGG - chr7.4:15235342-15235361 None:intergenic 35.0%
GTAAAAAATGCGTTGCCATT+CGG + chr7.4:15235418-15235437 MS.gene055961:CDS 35.0%
TAAAAAATGCGTTGCCATTC+GGG + chr7.4:15235419-15235438 MS.gene055961:CDS 35.0%
TCTATATCACAAGAGGAGAT+GGG + chr7.4:15235526-15235545 MS.gene055961:CDS 35.0%
TTCACTGATGGAGATTTCAA+TGG + chr7.4:15235574-15235593 MS.gene055961:CDS 35.0%
TTCTATATCACAAGAGGAGA+TGG + chr7.4:15235525-15235544 MS.gene055961:CDS 35.0%
! ACAAATACCATGCTAGGTTT+TGG - chr7.4:15235379-15235398 None:intergenic 35.0%
! GGAAGTACACTTAGCATATT+GGG + chr7.4:15235595-15235614 MS.gene055961:CDS 35.0%
! TGGAAGTACACTTAGCATAT+TGG + chr7.4:15235594-15235613 MS.gene055961:CDS 35.0%
!!! GCATTTTTTACCTTGCCATT+GGG - chr7.4:15235409-15235428 None:intergenic 35.0%
ACAATGGCCATTTCTCTAAC+AGG - chr7.4:15235643-15235662 None:intergenic 40.0%
GAGTGAACCTGTTAGAGAAA+TGG + chr7.4:15235633-15235652 MS.gene055961:CDS 40.0%
GTTAGAGAAATGGCCATTGT+TGG + chr7.4:15235643-15235662 MS.gene055961:CDS 40.0%
TACTTTCACGACATTGTGTC+AGG + chr7.4:15235352-15235371 MS.gene055961:CDS 40.0%
TCAGCTACAAATACCATGCT+AGG - chr7.4:15235385-15235404 None:intergenic 40.0%
! ACTAATGATGGTGATGACCA+TGG + chr7.4:15235549-15235568 MS.gene055961:CDS 40.0%
!!! CGCATTTTTTACCTTGCCAT+TGG - chr7.4:15235410-15235429 None:intergenic 40.0%
AAACTTGTGGGTAAGGCTCA+AGG + chr7.4:15235493-15235512 MS.gene055961:CDS 45.0%
AGAACGTGACTCGAAACTTG+TGG + chr7.4:15235480-15235499 MS.gene055961:CDS 45.0%
AGAGAAATGGCCATTGTTGG+TGG + chr7.4:15235646-15235665 MS.gene055961:CDS 45.0%
AGAGGAGATGGGACTAATGA+TGG + chr7.4:15235537-15235556 MS.gene055961:CDS 45.0%
ATCTCCATCAGTGAATGCCA+TGG - chr7.4:15235569-15235588 None:intergenic 45.0%
ATGACCATGGCATTCACTGA+TGG + chr7.4:15235562-15235581 MS.gene055961:CDS 45.0%
CACTAAAGGAGATGCCATTG+TGG + chr7.4:15235729-15235748 MS.gene055961:CDS 45.0%
GAACGTGACTCGAAACTTGT+GGG + chr7.4:15235481-15235500 MS.gene055961:CDS 45.0%
GAGAAATGGCCATTGTTGGT+GGG + chr7.4:15235647-15235666 MS.gene055961:CDS 45.0%
GTATTTGTAGCTGAGCCCAA+TGG + chr7.4:15235391-15235410 MS.gene055961:CDS 45.0%
TGACTCGAAACTTGTGGGTA+AGG + chr7.4:15235486-15235505 MS.gene055961:CDS 45.0%
! ACAAGTTTCGAGTCACGTTC+TGG - chr7.4:15235481-15235500 None:intergenic 45.0%
! GAGCTTTTCGTTTCGTACGT+GGG + chr7.4:15235674-15235693 MS.gene055961:CDS 45.0%
AAATGCGTTGCCATTCGGGA+CGG + chr7.4:15235423-15235442 MS.gene055961:CDS 50.0%
GTCAGGACCAAAACCTAGCA+TGG + chr7.4:15235369-15235388 MS.gene055961:CDS 50.0%
TTTCGTACGTGGGTATGCTG+AGG + chr7.4:15235684-15235703 MS.gene055961:CDS 50.0%
! GGAGCTTTTCGTTTCGTACG+TGG + chr7.4:15235673-15235692 MS.gene055961:CDS 50.0%
AGCTCCAGTCCCACCAACAA+TGG - chr7.4:15235659-15235678 None:intergenic 55.0%
ATGGACGATCCATTGACGGC+TGG + chr7.4:15235454-15235473 MS.gene055961:CDS 55.0%
GGCGATGGACGATCCATTGA+CGG + chr7.4:15235450-15235469 MS.gene055961:CDS 55.0%
TGGACGATCCATTGACGGCT+GGG + chr7.4:15235455-15235474 MS.gene055961:CDS 55.0%
TGTAGCTGAGCCCAATGGCA+AGG + chr7.4:15235396-15235415 MS.gene055961:CDS 55.0%
! ATGGCCATTGTTGGTGGGAC+TGG + chr7.4:15235652-15235671 MS.gene055961:CDS 55.0%
ATCGCCACAACCGTCCCGAA+TGG - chr7.4:15235436-15235455 None:intergenic 60.0%
ATTCGGGACGGTTGTGGCGA+TGG + chr7.4:15235435-15235454 MS.gene055961:CDS 60.0%
GTTGCCATTCGGGACGGTTG+TGG + chr7.4:15235429-15235448 MS.gene055961:CDS 60.0%
CGTTCTGGCCCAGCCGTCAA+TGG - chr7.4:15235466-15235485 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 15235310 15235780 15235310 ID=MS.gene055961
chr7.4 mRNA 15235310 15235780 15235310 ID=MS.gene055961.t1;Parent=MS.gene055961
chr7.4 exon 15235310 15235780 15235310 ID=MS.gene055961.t1.exon1;Parent=MS.gene055961.t1
chr7.4 CDS 15235310 15235780 15235310 ID=cds.MS.gene055961.t1;Parent=MS.gene055961.t1
Gene Sequence

>MS.gene055961

ATGTTGGGTTTTCAAGAAGAGAAATTCACCCATCTTCACTTTTACTTTCACGACATTGTGTCAGGACCAAAACCTAGCATGGTATTTGTAGCTGAGCCCAATGGCAAGGTAAAAAATGCGTTGCCATTCGGGACGGTTGTGGCGATGGACGATCCATTGACGGCTGGGCCAGAACGTGACTCGAAACTTGTGGGTAAGGCTCAAGGAATTTACACTTCTATATCACAAGAGGAGATGGGACTAATGATGGTGATGACCATGGCATTCACTGATGGAGATTTCAATGGAAGTACACTTAGCATATTGGGCAGAAACATGATCATGAGTGAACCTGTTAGAGAAATGGCCATTGTTGGTGGGACTGGAGCTTTTCGTTTCGTACGTGGGTATGCTGAGGCTAAGTTTTATTCCGTTGATTACACTAAAGGAGATGCCATTGTGGAATATGACATATTTGTGTTCCATTATTAG

Protein sequence

>MS.gene055961.t1

MLGFQEEKFTHLHFYFHDIVSGPKPSMVFVAEPNGKVKNALPFGTVVAMDDPLTAGPERDSKLVGKAQGIYTSISQEEMGLMMVMTMAFTDGDFNGSTLSILGRNMIMSEPVREMAIVGGTGAFRFVRGYAEAKFYSVDYTKGDAIVEYDIFVFHY