AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20576


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20576.t1 MTR_6g008900 97.491 279 7 0 1 279 93 371 0.0 575
MS.gene20576.t1 MTR_4g071620 97.491 279 7 0 1 279 93 371 0.0 575
MS.gene20576.t1 MTR_5g036580 68.519 270 83 1 1 270 94 361 4.28e-140 400
MS.gene20576.t1 MTR_7g109210 66.045 268 82 3 1 268 93 351 3.75e-134 384
MS.gene20576.t1 MTR_3g092310 61.423 267 98 3 2 268 71 332 5.55e-124 357
MS.gene20576.t1 MTR_7g094230 60.949 274 100 3 1 274 83 349 9.26e-123 354
MS.gene20576.t1 MTR_8g078240 59.551 267 104 1 1 267 74 336 2.55e-121 350
MS.gene20576.t1 MTR_3g111600 61.423 267 97 2 1 267 12 272 6.41e-121 347
MS.gene20576.t1 MTR_1g021855 62.313 268 94 3 1 267 97 358 5.79e-120 348
MS.gene20576.t1 MTR_5g019220 59.176 267 107 1 1 267 94 358 3.11e-119 346
MS.gene20576.t1 MTR_4g118030 58.736 269 103 4 1 268 77 338 9.29e-117 339
MS.gene20576.t1 MTR_1g089820 61.151 278 98 5 1 278 86 353 5.17e-115 335
MS.gene20576.t1 MTR_2g461670 34.239 184 109 6 73 254 395 568 2.78e-25 105
MS.gene20576.t1 MTR_4g109890 33.333 201 122 7 73 272 340 529 7.19e-24 100
MS.gene20576.t1 MTR_2g015550 31.401 207 124 7 73 279 339 527 5.59e-22 95.5
MS.gene20576.t1 MTR_8g092000 32.000 175 111 5 73 247 384 550 1.34e-21 94.7
MS.gene20576.t1 MTR_7g055600 31.000 200 123 7 54 247 214 404 2.29e-21 93.2
MS.gene20576.t1 MTR_7g057530 31.000 200 123 7 54 247 214 404 2.29e-21 93.2
MS.gene20576.t1 MTR_8g033360 32.620 187 116 4 44 225 403 584 2.38e-20 90.9
MS.gene20576.t1 MTR_4g133570 31.000 200 120 8 73 265 261 449 2.68e-20 90.5
MS.gene20576.t1 MTR_7g075840 29.412 204 127 6 73 276 468 654 3.26e-20 90.5
MS.gene20576.t1 MTR_3g107930 29.245 212 137 5 44 250 358 561 3.83e-20 90.5
MS.gene20576.t1 MTR_3g107930 29.245 212 137 5 44 250 435 638 3.97e-20 90.5
MS.gene20576.t1 MTR_7g012370 28.729 181 116 6 73 247 334 507 3.42e-19 87.4
MS.gene20576.t1 MTR_6g016380 28.261 230 147 8 28 247 304 525 4.08e-19 87.4
MS.gene20576.t1 MTR_2g082650 29.778 225 137 10 51 265 184 397 6.38e-19 86.3
MS.gene20576.t1 MTR_2g027740 31.461 178 108 5 73 247 471 637 9.37e-19 86.3
MS.gene20576.t1 MTR_2g082650 29.778 225 137 10 51 265 313 526 1.14e-18 85.9
MS.gene20576.t1 MTR_3g452360 28.272 191 125 6 64 247 166 351 2.36e-15 75.5
MS.gene20576.t1 MTR_3g452360 28.272 191 125 6 64 247 315 500 4.90e-15 75.1
MS.gene20576.t1 MTR_7g096160 25.143 175 126 3 73 247 455 624 5.10e-14 72.4
MS.gene20576.t1 MTR_6g013840 29.530 149 97 4 103 247 358 502 9.21e-14 71.2
MS.gene20576.t1 MTR_2g006790 27.053 207 109 8 73 279 339 503 1.52e-12 67.8
MS.gene20576.t1 MTR_7g074680 31.928 166 92 9 103 265 403 550 5.62e-12 66.2
MS.gene20576.t1 MTR_7g074680 31.928 166 92 9 103 265 443 590 6.13e-12 65.9
MS.gene20576.t1 MTR_7g074680 31.928 166 92 9 103 265 463 610 6.87e-12 65.9
MS.gene20576.t1 MTR_7g074680 31.928 166 92 9 103 265 463 610 7.61e-12 65.9
MS.gene20576.t1 MTR_7g074680 31.928 166 92 9 103 265 500 647 7.92e-12 65.9
MS.gene20576.t1 MTR_7g074680 31.928 166 92 9 103 265 483 630 8.68e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20576.t1 AT3G28340 66.909 275 85 3 1 274 86 355 4.26e-143 407
MS.gene20576.t1 AT1G70090 68.657 268 82 1 1 268 97 362 5.65e-141 402
MS.gene20576.t1 AT1G70090 68.657 268 82 1 1 268 97 362 5.65e-141 402
MS.gene20576.t1 AT1G24170 67.164 268 86 1 1 268 100 365 3.38e-138 395
MS.gene20576.t1 AT3G62660 67.537 268 78 3 1 268 98 356 7.58e-134 383
MS.gene20576.t1 AT4G02130 66.791 268 83 3 1 268 81 342 1.38e-132 379
MS.gene20576.t1 AT4G02130 66.791 268 83 3 1 268 81 342 1.38e-132 379
MS.gene20576.t1 AT4G02130 66.791 268 83 3 1 268 81 342 1.38e-132 379
MS.gene20576.t1 AT1G02720 63.569 269 90 5 1 268 96 357 4.34e-125 361
MS.gene20576.t1 AT1G02720 63.569 269 90 5 1 268 96 357 4.34e-125 361
MS.gene20576.t1 AT3G06260 62.313 268 95 5 1 268 81 342 2.34e-123 356
MS.gene20576.t1 AT3G50760 59.259 270 101 3 1 267 68 331 6.00e-117 339
MS.gene20576.t1 AT1G13250 61.194 268 98 3 1 268 75 336 1.31e-112 328
MS.gene20576.t1 AT1G19300 62.921 267 95 2 1 267 80 342 6.09e-112 327
MS.gene20576.t1 AT1G18580 29.078 282 157 11 4 254 246 515 2.21e-26 108
MS.gene20576.t1 AT3G25140 30.348 201 123 6 54 247 339 529 2.89e-22 96.7
MS.gene20576.t1 AT4G38270 30.144 209 133 5 44 247 446 646 5.56e-22 95.9
MS.gene20576.t1 AT4G38270 30.144 209 133 5 44 247 450 650 5.68e-22 95.9
MS.gene20576.t1 AT5G47780 33.143 175 109 5 73 247 420 586 3.97e-21 93.2
MS.gene20576.t1 AT3G61130 30.342 234 141 8 28 250 424 646 5.25e-21 93.2
MS.gene20576.t1 AT3G01040 28.571 189 124 6 73 255 329 512 2.35e-20 90.9
MS.gene20576.t1 AT3G01040 28.571 189 124 6 73 255 329 512 2.35e-20 90.9
MS.gene20576.t1 AT3G01040 28.571 189 124 6 73 255 296 479 2.53e-20 90.9
MS.gene20576.t1 AT5G15470 28.796 191 121 7 73 255 329 512 2.28e-19 88.2
MS.gene20576.t1 AT3G58790 30.000 180 116 6 73 247 204 378 3.04e-19 87.0
MS.gene20576.t1 AT3G58790 29.050 179 119 5 73 247 336 510 5.82e-19 86.7
MS.gene20576.t1 AT5G54690 28.492 179 119 5 73 247 331 504 8.42e-19 86.3
MS.gene20576.t1 AT3G02350 31.100 209 130 6 44 247 332 531 8.33e-18 83.6
MS.gene20576.t1 AT2G30575 30.286 175 117 3 73 247 411 580 8.56e-18 83.6
MS.gene20576.t1 AT2G46480 30.899 178 115 5 73 250 344 513 1.33e-17 82.8
MS.gene20576.t1 AT2G46480 30.899 178 115 5 73 250 357 526 1.37e-17 82.8
MS.gene20576.t1 AT1G06780 28.571 175 120 3 73 247 403 572 1.43e-16 79.7
MS.gene20576.t1 AT1G06780 28.571 175 120 3 73 247 390 559 1.87e-16 79.3
MS.gene20576.t1 AT2G20810 34.831 178 107 5 73 250 342 510 1.96e-15 76.3

Find 47 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATGATATTCAAGATTTA+TGG 0.160378 6.3:-4690283 MS.gene20576:CDS
ATATGGACATTCATTGATTT+AGG 0.240908 6.3:-4689766 None:intergenic
TATATGCCTCATGTGGGATA+TGG 0.327711 6.3:-4689783 MS.gene20576:CDS
CTCCAATGTAACAAACTAAC+TGG 0.371144 6.3:+4689885 None:intergenic
TGTTACATTGGAGTGGTAAA+GGG 0.388590 6.3:-4689875 MS.gene20576:CDS
TCAAGTGCTTGTCTTATTGA+AGG 0.389187 6.3:+4690398 None:intergenic
TTGAAGCTATTGAACATAGA+TGG 0.398424 6.3:-4689968 MS.gene20576:CDS
AGAATAAAGATGAGTTTACT+AGG 0.399390 6.3:-4690499 MS.gene20576:CDS
GTGAGAAGGGTTTATAAGCT+TGG 0.416693 6.3:-4690029 MS.gene20576:CDS
TTGTTACATTGGAGTGGTAA+AGG 0.425011 6.3:-4689876 MS.gene20576:CDS
GTCCAGTTAGTTTGTTACAT+TGG 0.426248 6.3:-4689887 MS.gene20576:CDS
GAGTTTACTAGGATTGTTCA+TGG 0.433334 6.3:-4690488 MS.gene20576:CDS
TTATGGAAGGTTTCGTTGAC+CGG 0.444509 6.3:-4690266 MS.gene20576:CDS
AAATTTGCATGACAATATTC+CGG 0.448533 6.3:+4690224 None:intergenic
ACACAAAGAAGATTGAGAAA+TGG 0.449104 6.3:-4690067 MS.gene20576:CDS
TGGTAATGGATTTGGTGAAA+TGG 0.449678 6.3:-4690103 MS.gene20576:CDS
CAACACTGGTGTGATGGTAA+TGG 0.479656 6.3:-4690117 MS.gene20576:CDS
TAAACTTTGAATTTCAATGA+AGG 0.482539 6.3:+4690458 None:intergenic
TAACTTCTTGCATAATTCAA+AGG 0.483151 6.3:+4690371 None:intergenic
TGAGGCATATAAAGATCATA+AGG 0.488978 6.3:+4689795 None:intergenic
TGGTGTGATGGTAATGGATT+TGG 0.491814 6.3:-4690111 MS.gene20576:CDS
AGATGGAATCAACATGGTCT+TGG 0.496429 6.3:-4689951 MS.gene20576:CDS
GAAATGGATGGAAATTCAAA+AGG 0.498984 6.3:-4690051 MS.gene20576:CDS
GGAAATTCAAAAGGTGAGAA+GGG 0.517101 6.3:-4690042 MS.gene20576:CDS
TTGACCGGATCGAAGGTAAT+CGG 0.520100 6.3:-4690251 MS.gene20576:CDS
ATTGCATCAAGTCTTCTCCA+TGG 0.528733 6.3:+4689849 None:intergenic
TGGAAATTCAAAAGGTGAGA+AGG 0.532785 6.3:-4690043 MS.gene20576:CDS
TGATCTTTATATGCCTCATG+TGG 0.542660 6.3:-4689790 MS.gene20576:CDS
AGTTGTAGAAGTTTGCATCC+TGG 0.544023 6.3:-4689909 MS.gene20576:CDS
GGTTTCGTTGACCGGATCGA+AGG 0.548154 6.3:-4690258 MS.gene20576:CDS
AAAGAAGATTGAGAAATGGA+TGG 0.561407 6.3:-4690063 MS.gene20576:CDS
GTTAGTTTGTTACATTGGAG+TGG 0.566455 6.3:-4689882 MS.gene20576:CDS
ATCGAAGGTAATCGGTGCAC+CGG 0.567159 6.3:-4690243 MS.gene20576:CDS
TGGAATCAACATGGTCTTGG+TGG 0.582134 6.3:-4689948 MS.gene20576:CDS
AAGCCATGTTATTTCAACAC+TGG 0.583969 6.3:-4690131 MS.gene20576:CDS
TGCACCGATTACCTTCGATC+CGG 0.596738 6.3:+4690247 None:intergenic
TGTAACAAACTAACTGGACC+AGG 0.606289 6.3:+4689891 None:intergenic
TGATATTCAAGATTTATGGA+AGG 0.616697 6.3:-4690279 MS.gene20576:CDS
GATCTTTATATGCCTCATGT+GGG 0.625084 6.3:-4689789 MS.gene20576:CDS
TTATTTCAACACTGGTGTGA+TGG 0.626622 6.3:-4690123 MS.gene20576:CDS
ACACCAGTGTTGAAATAACA+TGG 0.638418 6.3:+4690128 None:intergenic
GAATGTCCATATCCCACATG+AGG 0.644433 6.3:+4689777 None:intergenic
GGAGAAGACTTGATGCAATG+AGG 0.649459 6.3:-4689845 MS.gene20576:CDS
GATTTGGTGAAATGGAGAGA+AGG 0.660269 6.3:-4690095 MS.gene20576:CDS
GGAGTGGTAAAGGGAAACCA+TGG 0.670933 6.3:-4689866 MS.gene20576:CDS
ATTGTCATGCAAATTTCACA+AGG 0.680182 6.3:-4690217 MS.gene20576:CDS
GAACATAGATGGAATCAACA+TGG 0.707717 6.3:-4689957 MS.gene20576:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAAAAAACAAATTCTT+AGG + chr6.3:4689809-4689828 None:intergenic 15.0%
!! TAAACTTTGAATTTCAATGA+AGG + chr6.3:4689896-4689915 None:intergenic 20.0%
!!! TTGATGATATTCAAGATTTA+TGG - chr6.3:4690068-4690087 MS.gene20576:CDS 20.0%
! AAATTTGCATGACAATATTC+CGG + chr6.3:4690130-4690149 None:intergenic 25.0%
! AGAATAAAGATGAGTTTACT+AGG - chr6.3:4689852-4689871 MS.gene20576:CDS 25.0%
! TAACTTCTTGCATAATTCAA+AGG + chr6.3:4689983-4690002 None:intergenic 25.0%
! TGATATTCAAGATTTATGGA+AGG - chr6.3:4690072-4690091 MS.gene20576:CDS 25.0%
AAAGAAGATTGAGAAATGGA+TGG - chr6.3:4690288-4690307 MS.gene20576:CDS 30.0%
ACACAAAGAAGATTGAGAAA+TGG - chr6.3:4690284-4690303 MS.gene20576:CDS 30.0%
ATTGTCATGCAAATTTCACA+AGG - chr6.3:4690134-4690153 MS.gene20576:CDS 30.0%
CACAAAAAATCAACAGAACA+AGG + chr6.3:4690532-4690551 None:intergenic 30.0%
GAAATGGATGGAAATTCAAA+AGG - chr6.3:4690300-4690319 MS.gene20576:CDS 30.0%
TGAGGCATATAAAGATCATA+AGG + chr6.3:4690559-4690578 None:intergenic 30.0%
TTGAAGCTATTGAACATAGA+TGG - chr6.3:4690383-4690402 MS.gene20576:CDS 30.0%
! GTCAAGTTATGAATTTTCTG+AGG - chr6.3:4690177-4690196 MS.gene20576:CDS 30.0%
!!! ATCATTACCTCCTTTTTTGA+TGG - chr6.3:4690345-4690364 MS.gene20576:CDS 30.0%
!!! CTTGTTCTGTTGATTTTTTG+TGG - chr6.3:4690530-4690549 MS.gene20576:CDS 30.0%
!!! GAATTTTCTGAGGTGTTTAA+AGG - chr6.3:4690187-4690206 MS.gene20576:CDS 30.0%
!!! GGTATTTTAGCTATGAGTTT+TGG - chr6.3:4690155-4690174 MS.gene20576:CDS 30.0%
CCAAAAACCATCAAAAAAGG+AGG + chr6.3:4690355-4690374 None:intergenic 35.0%
CCACCAAAAACCATCAAAAA+AGG + chr6.3:4690358-4690377 None:intergenic 35.0%
CTCCAATGTAACAAACTAAC+TGG + chr6.3:4690469-4690488 None:intergenic 35.0%
GAACATAGATGGAATCAACA+TGG - chr6.3:4690394-4690413 MS.gene20576:CDS 35.0%
GAGTTTACTAGGATTGTTCA+TGG - chr6.3:4689863-4689882 MS.gene20576:CDS 35.0%
GATCTTTATATGCCTCATGT+GGG - chr6.3:4690562-4690581 MS.gene20576:CDS 35.0%
GGAAATTCAAAAGGTGAGAA+GGG - chr6.3:4690309-4690328 MS.gene20576:CDS 35.0%
TGATCTTTATATGCCTCATG+TGG - chr6.3:4690561-4690580 MS.gene20576:CDS 35.0%
TGGAAATTCAAAAGGTGAGA+AGG - chr6.3:4690308-4690327 MS.gene20576:CDS 35.0%
TGTTACATTGGAGTGGTAAA+GGG - chr6.3:4690476-4690495 MS.gene20576:CDS 35.0%
TTATTTCAACACTGGTGTGA+TGG - chr6.3:4690228-4690247 MS.gene20576:CDS 35.0%
TTGTTACATTGGAGTGGTAA+AGG - chr6.3:4690475-4690494 MS.gene20576:CDS 35.0%
! ACACCAGTGTTGAAATAACA+TGG + chr6.3:4690226-4690245 None:intergenic 35.0%
! TCAAGTGCTTGTCTTATTGA+AGG + chr6.3:4689956-4689975 None:intergenic 35.0%
!! AAGCCATGTTATTTCAACAC+TGG - chr6.3:4690220-4690239 MS.gene20576:CDS 35.0%
!! GTCCAGTTAGTTTGTTACAT+TGG - chr6.3:4690464-4690483 MS.gene20576:CDS 35.0%
!! GTTAGTTTGTTACATTGGAG+TGG - chr6.3:4690469-4690488 MS.gene20576:CDS 35.0%
!! TGGTAATGGATTTGGTGAAA+TGG - chr6.3:4690248-4690267 MS.gene20576:CDS 35.0%
!!! CCTCCTTTTTTGATGGTTTT+TGG - chr6.3:4690352-4690371 MS.gene20576:CDS 35.0%
!!! CCTTTTTTGATGGTTTTTGG+TGG - chr6.3:4690355-4690374 MS.gene20576:CDS 35.0%
AGATGGAATCAACATGGTCT+TGG - chr6.3:4690400-4690419 MS.gene20576:CDS 40.0%
AGTTGTAGAAGTTTGCATCC+TGG - chr6.3:4690442-4690461 MS.gene20576:CDS 40.0%
ATTGCATCAAGTCTTCTCCA+TGG + chr6.3:4690505-4690524 None:intergenic 40.0%
GTGAGAAGGGTTTATAAGCT+TGG - chr6.3:4690322-4690341 MS.gene20576:CDS 40.0%
TATATGCCTCATGTGGGATA+TGG - chr6.3:4690568-4690587 MS.gene20576:CDS 40.0%
TGTAACAAACTAACTGGACC+AGG + chr6.3:4690463-4690482 None:intergenic 40.0%
TTATGGAAGGTTTCGTTGAC+CGG - chr6.3:4690085-4690104 MS.gene20576:CDS 40.0%
!! GATTTGGTGAAATGGAGAGA+AGG - chr6.3:4690256-4690275 MS.gene20576:CDS 40.0%
!! TGGTGTGATGGTAATGGATT+TGG - chr6.3:4690240-4690259 MS.gene20576:CDS 40.0%
CAACACTGGTGTGATGGTAA+TGG - chr6.3:4690234-4690253 MS.gene20576:CDS 45.0%
GAATGTCCATATCCCACATG+AGG + chr6.3:4690577-4690596 None:intergenic 45.0%
GGAGAAGACTTGATGCAATG+AGG - chr6.3:4690506-4690525 MS.gene20576:CDS 45.0%
TTGACCGGATCGAAGGTAAT+CGG - chr6.3:4690100-4690119 MS.gene20576:CDS 45.0%
! TGGAATCAACATGGTCTTGG+TGG - chr6.3:4690403-4690422 MS.gene20576:CDS 45.0%
ATCGAAGGTAATCGGTGCAC+CGG - chr6.3:4690108-4690127 MS.gene20576:CDS 50.0%
GGAGTGGTAAAGGGAAACCA+TGG - chr6.3:4690485-4690504 MS.gene20576:CDS 50.0%
!! TGCACCGATTACCTTCGATC+CGG + chr6.3:4690107-4690126 None:intergenic 50.0%
GGTTTCGTTGACCGGATCGA+AGG - chr6.3:4690093-4690112 MS.gene20576:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 4689767 4690606 4689767 ID=MS.gene20576
chr6.3 mRNA 4689767 4690606 4689767 ID=MS.gene20576.t1;Parent=MS.gene20576
chr6.3 exon 4689767 4690606 4689767 ID=MS.gene20576.t1.exon1;Parent=MS.gene20576.t1
chr6.3 CDS 4689767 4690606 4689767 ID=cds.MS.gene20576.t1;Parent=MS.gene20576.t1
Gene Sequence

>MS.gene20576

ATGGCTGCAATTCATTCTGTTCTCAAACACACTTCATGTCCTAAGAATTTGTTTTTTCATTTCATTGCATCAGATTCAAGATTAGAGAATAAAGATGAGTTTACTAGGATTGTTCATGGTTCATTTCCTTCATTGAAATTCAAAGTTTATGTTTTCAATGAAAGTTTAGTTGAGAATCTTATATCTCCTTCAATAAGACAAGCACTTGAGAATCCTTTGAATTATGCAAGAAGTTATTTAGCTGATTTACTTGAAGAATGTGTTGAGAGAGTTATCTATTTAGATTCTGATATTATAGTTGTTGATGATATTCAAGATTTATGGAAGGTTTCGTTGACCGGATCGAAGGTAATCGGTGCACCGGAATATTGTCATGCAAATTTCACAAGGTATTTTAGCTATGAGTTTTGGTCAAGTTATGAATTTTCTGAGGTGTTTAAAGGAAGAAAGAATAAGCCATGTTATTTCAACACTGGTGTGATGGTAATGGATTTGGTGAAATGGAGAGAAGGTGAATACACAAAGAAGATTGAGAAATGGATGGAAATTCAAAAGGTGAGAAGGGTTTATAAGCTTGGATCATTACCTCCTTTTTTGATGGTTTTTGGTGGTGATATTGAAGCTATTGAACATAGATGGAATCAACATGGTCTTGGTGGTGATAATGTTGTTGATAGTTGTAGAAGTTTGCATCCTGGTCCAGTTAGTTTGTTACATTGGAGTGGTAAAGGGAAACCATGGAGAAGACTTGATGCAATGAGGCCTTGTTCTGTTGATTTTTTGTGGAAACCTTATGATCTTTATATGCCTCATGTGGGATATGGACATTCATTGATTTAG

Protein sequence

>MS.gene20576.t1

MAAIHSVLKHTSCPKNLFFHFIASDSRLENKDEFTRIVHGSFPSLKFKVYVFNESLVENLISPSIRQALENPLNYARSYLADLLEECVERVIYLDSDIIVVDDIQDLWKVSLTGSKVIGAPEYCHANFTRYFSYEFWSSYEFSEVFKGRKNKPCYFNTGVMVMDLVKWREGEYTKKIEKWMEIQKVRRVYKLGSLPPFLMVFGGDIEAIEHRWNQHGLGGDNVVDSCRSLHPGPVSLLHWSGKGKPWRRLDAMRPCSVDFLWKPYDLYMPHVGYGHSLI