Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051837.t1 | XP_024637181.1 | 86.4 | 487 | 59 | 3 | 1 | 483 | 1 | 484 | 6.90E-229 | 803.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051837.t1 | Q9M2U3 | 28.4 | 324 | 193 | 9 | 143 | 439 | 53 | 364 | 3.6e-32 | 141.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051837.t1 | A0A396IIE8 | 86.4 | 487 | 59 | 3 | 1 | 483 | 1 | 484 | 5.0e-229 | 803.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049160 | MS.gene051837 | 0.806837 | 6.78E-50 | -1.69E-46 |
| MS.gene04998 | MS.gene051837 | 0.830133 | 3.49E-55 | -1.69E-46 |
| MS.gene051226 | MS.gene051837 | 0.865056 | 7.79E-65 | -1.69E-46 |
| MS.gene051837 | MS.gene051840 | 0.821159 | 4.68E-53 | -1.69E-46 |
| MS.gene051837 | MS.gene053499 | 0.839201 | 1.83E-57 | -1.69E-46 |
| MS.gene051837 | MS.gene053501 | 0.804443 | 2.15E-49 | -1.69E-46 |
| MS.gene051837 | MS.gene054617 | 0.842003 | 3.37E-58 | -1.69E-46 |
| MS.gene051837 | MS.gene057093 | 0.822266 | 2.60E-53 | -1.69E-46 |
| MS.gene051837 | MS.gene057422 | 0.81827 | 2.14E-52 | -1.69E-46 |
| MS.gene051837 | MS.gene057432 | 0.856135 | 3.95E-62 | -1.69E-46 |
| MS.gene051837 | MS.gene06061 | 0.846101 | 2.68E-59 | -1.69E-46 |
| MS.gene051837 | MS.gene061543 | 0.85127 | 9.90E-61 | -1.69E-46 |
| MS.gene051837 | MS.gene061546 | 0.836798 | 7.57E-57 | -1.69E-46 |
| MS.gene051837 | MS.gene067477 | 0.851829 | 6.88E-61 | -1.69E-46 |
| MS.gene051837 | MS.gene069576 | 0.811873 | 5.63E-51 | -1.69E-46 |
| MS.gene051837 | MS.gene070072 | 0.814105 | 1.82E-51 | -1.69E-46 |
| MS.gene051837 | MS.gene070320 | 0.814813 | 1.27E-51 | -1.69E-46 |
| MS.gene051837 | MS.gene09595 | 0.806373 | 8.49E-50 | -1.69E-46 |
| MS.gene051837 | MS.gene20293 | 0.807072 | 6.04E-50 | -1.69E-46 |
| MS.gene051837 | MS.gene20576 | 0.848416 | 6.22E-60 | -1.69E-46 |
| MS.gene051837 | MS.gene22541 | 0.876813 | 1.04E-68 | -1.69E-46 |
| MS.gene051837 | MS.gene22543 | 0.878369 | 2.97E-69 | -1.69E-46 |
| MS.gene051837 | MS.gene24332 | 0.826639 | 2.43E-54 | -1.69E-46 |
| MS.gene051837 | MS.gene24855 | 0.96072 | 7.07E-119 | -1.69E-46 |
| MS.gene051837 | MS.gene24856 | 0.980865 | 3.24E-151 | -1.69E-46 |
| MS.gene051837 | MS.gene24859 | 0.826739 | 2.30E-54 | -1.69E-46 |
| MS.gene051837 | MS.gene26139 | 0.820992 | 5.12E-53 | -1.69E-46 |
| MS.gene051837 | MS.gene26489 | 0.81525 | 1.02E-51 | -1.69E-46 |
| MS.gene051837 | MS.gene26630 | 0.833355 | 5.59E-56 | -1.69E-46 |
| MS.gene051837 | MS.gene26632 | 0.839782 | 1.29E-57 | -1.69E-46 |
| MS.gene051837 | MS.gene26633 | 0.863632 | 2.17E-64 | -1.69E-46 |
| MS.gene051837 | MS.gene29584 | 0.821574 | 3.76E-53 | -1.69E-46 |
| MS.gene051837 | MS.gene29657 | 0.819288 | 1.26E-52 | -1.69E-46 |
| MS.gene051837 | MS.gene35487 | 0.839868 | 1.22E-57 | -1.69E-46 |
| MS.gene051837 | MS.gene36448 | 0.803939 | 2.74E-49 | -1.69E-46 |
| MS.gene051837 | MS.gene37912 | 0.842717 | 2.18E-58 | -1.69E-46 |
| MS.gene051837 | MS.gene39643 | 0.84448 | 7.36E-59 | -1.69E-46 |
| MS.gene051837 | MS.gene39645 | 0.829849 | 4.09E-55 | -1.69E-46 |
| MS.gene051837 | MS.gene39646 | 0.811697 | 6.15E-51 | -1.69E-46 |
| MS.gene051837 | MS.gene41022 | 0.811563 | 6.58E-51 | -1.69E-46 |
| MS.gene051837 | MS.gene45309 | 0.824324 | 8.58E-54 | -1.69E-46 |
| MS.gene051837 | MS.gene46918 | 0.846304 | 2.36E-59 | -1.69E-46 |
| MS.gene051837 | MS.gene48476 | 0.81405 | 1.88E-51 | -1.69E-46 |
| MS.gene051837 | MS.gene51404 | 0.823148 | 1.62E-53 | -1.69E-46 |
| MS.gene051837 | MS.gene54188 | 0.859255 | 4.69E-63 | -1.69E-46 |
| MS.gene051837 | MS.gene54738 | 0.855246 | 7.18E-62 | -1.69E-46 |
| MS.gene051837 | MS.gene56695 | 0.823903 | 1.08E-53 | -1.69E-46 |
| MS.gene051837 | MS.gene57009 | 0.8359 | 1.28E-56 | -1.69E-46 |
| MS.gene051837 | MS.gene57601 | 0.983663 | 2.31E-158 | -1.69E-46 |
| MS.gene051837 | MS.gene57704 | 0.804916 | 1.72E-49 | -1.69E-46 |
| MS.gene051837 | MS.gene58305 | 0.810788 | 9.68E-51 | -1.69E-46 |
| MS.gene051837 | MS.gene58768 | 0.826249 | 3.01E-54 | -1.69E-46 |
| MS.gene051837 | MS.gene58769 | 0.821123 | 4.77E-53 | -1.69E-46 |
| MS.gene051837 | MS.gene58793 | 0.822512 | 2.28E-53 | -1.69E-46 |
| MS.gene051837 | MS.gene59219 | 0.816458 | 5.47E-52 | -1.69E-46 |
| MS.gene051837 | MS.gene61289 | 0.82328 | 1.51E-53 | -1.69E-46 |
| MS.gene051837 | MS.gene64870 | 0.854367 | 1.29E-61 | -1.69E-46 |
| MS.gene051837 | MS.gene65315 | 0.853982 | 1.67E-61 | -1.69E-46 |
| MS.gene051837 | MS.gene65380 | 0.801191 | 1.01E-48 | -1.69E-46 |
| MS.gene051837 | MS.gene71734 | 0.898658 | 4.28E-77 | -1.69E-46 |
| MS.gene051837 | MS.gene71987 | 0.840677 | 7.53E-58 | -1.69E-46 |
| MS.gene051837 | MS.gene72157 | 0.829519 | 4.92E-55 | -1.69E-46 |
| MS.gene051837 | MS.gene72160 | 0.805552 | 1.26E-49 | -1.69E-46 |
| MS.gene051837 | MS.gene72178 | 0.808318 | 3.28E-50 | -1.69E-46 |
| MS.gene051837 | MS.gene72639 | 0.832541 | 8.91E-56 | -1.69E-46 |
| MS.gene051837 | MS.gene73468 | 0.899228 | 2.44E-77 | -1.69E-46 |
| MS.gene051837 | MS.gene77362 | 0.810498 | 1.12E-50 | -1.69E-46 |
| MS.gene051837 | MS.gene81604 | 0.886817 | 2.46E-72 | -1.69E-46 |
| MS.gene051837 | MS.gene82041 | 0.817356 | 3.44E-52 | -1.69E-46 |
| MS.gene051837 | MS.gene82042 | 0.840303 | 9.43E-58 | -1.69E-46 |
| MS.gene051837 | MS.gene82549 | 0.850163 | 2.03E-60 | -1.69E-46 |
| MS.gene051837 | MS.gene83604 | 0.837431 | 5.22E-57 | -1.69E-46 |
| MS.gene051837 | MS.gene83618 | 0.883273 | 5.16E-71 | -1.69E-46 |
| MS.gene051837 | MS.gene86565 | 0.858415 | 8.37E-63 | -1.69E-46 |
| MS.gene051837 | MS.gene90362 | 0.848447 | 6.09E-60 | -1.69E-46 |
| MS.gene051837 | MS.gene92946 | 0.847158 | 1.38E-59 | -1.69E-46 |
| MS.gene051837 | MS.gene98294 | 0.800443 | 1.44E-48 | -1.69E-46 |
| MS.gene051837 | MS.gene98295 | 0.803574 | 3.27E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051837.t1 | AT5G12010 | 63.271 | 373 | 133 | 2 | 115 | 483 | 129 | 501 | 0.0 | 516 |
| MS.gene051837.t1 | AT4G29780 | 64.738 | 363 | 127 | 1 | 119 | 480 | 174 | 536 | 2.40e-178 | 511 |
| MS.gene051837.t1 | AT3G55350 | 28.395 | 324 | 193 | 9 | 143 | 439 | 53 | 364 | 7.98e-37 | 140 |
| MS.gene051837.t1 | AT3G63270 | 29.524 | 315 | 187 | 9 | 143 | 430 | 43 | 349 | 1.32e-36 | 139 |
| MS.gene051837.t1 | AT3G19120 | 24.839 | 310 | 193 | 8 | 155 | 442 | 114 | 405 | 3.29e-23 | 102 |
Find 117 sgRNAs with CRISPR-Local
Find 132 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTTTGATGGATGGAATTT+TGG | 0.058357 | 8.2:+6812979 | MS.gene051837:CDS |
| TCTTGGGTTGTTGGAAATTC+TGG | 0.088510 | 8.2:+6812953 | MS.gene051837:CDS |
| CTCTTTGAAACCAGCCTTAA+AGG | 0.137684 | 8.2:-6812527 | None:intergenic |
| ACATTTGGTTGTTCCATTTC+AGG | 0.175257 | 8.2:-6811960 | None:intergenic |
| TTGCCACCGGTGATCCTTTA+AGG | 0.178844 | 8.2:+6812513 | MS.gene051837:CDS |
| CATTTCTGCCACCAGTCTTT+TGG | 0.195355 | 8.2:-6812323 | None:intergenic |
| AACTCTTCCTCCGGAAATTC+TGG | 0.218374 | 8.2:-6812353 | None:intergenic |
| TTGGACTTAGGTGCAATTAT+TGG | 0.242423 | 8.2:-6812734 | None:intergenic |
| GAGGAGTTGCCTGGTGTTCT+TGG | 0.249284 | 8.2:+6813145 | MS.gene051837:CDS |
| AAACAGCTGATTCAAGTTCT+TGG | 0.262964 | 8.2:-6812421 | None:intergenic |
| TTCACCCATAACCGTCTTAC+TGG | 0.272495 | 8.2:-6812293 | None:intergenic |
| GTCAAATTCTGATGTGTATA+AGG | 0.284658 | 8.2:-6813004 | None:intergenic |
| CAGGGAATTTGAAAGATTCT+TGG | 0.293250 | 8.2:+6812936 | MS.gene051837:CDS |
| TTGCTAAGGGATGCAATTCC+CGG | 0.296879 | 8.2:+6812458 | MS.gene051837:CDS |
| CGAACGTTGCTTTGCTCATC+CGG | 0.309148 | 8.2:-6812388 | None:intergenic |
| CTTGGGTTGTTGGAAATTCT+GGG | 0.311033 | 8.2:+6812954 | MS.gene051837:CDS |
| TTATAATGATGATCAGGTTT+TGG | 0.315416 | 8.2:+6812883 | MS.gene051837:CDS |
| AAGAGTTCAGGAGATGTTTC+CGG | 0.316563 | 8.2:+6812369 | MS.gene051837:CDS |
| GTCATTGCAGTTGTTGTTGT+TGG | 0.330009 | 8.2:-6812051 | None:intergenic |
| GGTGATTGTTGTTGTGTTGT+TGG | 0.332434 | 8.2:-6812137 | None:intergenic |
| AGCGCGTGGCGGTTTGTATC+TGG | 0.334097 | 8.2:+6812486 | MS.gene051837:CDS |
| AAATTCTGGGTTCCCTTTGA+TGG | 0.337782 | 8.2:+6812967 | MS.gene051837:CDS |
| ATGCAGCAGTCCAGAATTTC+CGG | 0.340952 | 8.2:+6812343 | MS.gene051837:CDS |
| AACCGCCACGCGCTGCCGCC+CGG | 0.343618 | 8.2:-6812477 | None:intergenic |
| TCATTGCAGTTGTTGTTGTT+GGG | 0.357852 | 8.2:-6812050 | None:intergenic |
| TGAATTCTTATTTCAATAAG+AGG | 0.366055 | 8.2:+6812759 | MS.gene051837:CDS |
| TTGAAAGATTCTTGGGTTGT+TGG | 0.375328 | 8.2:+6812944 | MS.gene051837:CDS |
| CAAATCGAATGAGAGTATTA+AGG | 0.375622 | 8.2:-6812094 | None:intergenic |
| TACATTGAACCACCAACATT+TGG | 0.379629 | 8.2:-6812698 | None:intergenic |
| ACCGCCACGCGCTGCCGCCC+GGG | 0.388713 | 8.2:-6812476 | None:intergenic |
| CTGTGATTGTGATGAATCTT+CGG | 0.392333 | 8.2:-6812006 | None:intergenic |
| CATTTGGTTGTTCCATTTCA+GGG | 0.397112 | 8.2:-6811959 | None:intergenic |
| GGTTTCAAAGAGGTTCGGTT+TGG | 0.398582 | 8.2:+6812538 | MS.gene051837:CDS |
| GCATACTCTGTTACTGTTCA+AGG | 0.400851 | 8.2:+6812806 | MS.gene051837:CDS |
| TTATGCCAAAGTTTATTACT+TGG | 0.406688 | 8.2:+6812618 | MS.gene051837:CDS |
| AATTTCCGGAGGAAGAGTTC+AGG | 0.409085 | 8.2:+6812357 | MS.gene051837:CDS |
| TGGTTCTTATAATGATGATC+AGG | 0.413194 | 8.2:+6812877 | MS.gene051837:CDS |
| AGGGAATTTGAAAGATTCTT+GGG | 0.414339 | 8.2:+6812937 | MS.gene051837:CDS |
| ATCAAAATCAATCTCAAAGC+TGG | 0.418194 | 8.2:-6812180 | None:intergenic |
| GTTTCAAAGAGGTTCGGTTT+GGG | 0.421074 | 8.2:+6812539 | MS.gene051837:CDS |
| CACCGGTGATCCTTTAAGGC+TGG | 0.435269 | 8.2:+6812517 | MS.gene051837:CDS |
| CAGCAAGCACCAAGAACACC+AGG | 0.435403 | 8.2:-6813154 | None:intergenic |
| TGTGATTGTGATGAATCTTC+GGG | 0.436944 | 8.2:-6812005 | None:intergenic |
| GAGTTGTCCATTGTTGTTGT+TGG | 0.438490 | 8.2:-6812158 | None:intergenic |
| AAGGTGTGGTTGATACTAAA+GGG | 0.444687 | 8.2:+6812825 | MS.gene051837:CDS |
| TAAGAATTCACATTGGACTT+AGG | 0.445097 | 8.2:-6812746 | None:intergenic |
| AGGTCCAGTAAGACGGTTAT+GGG | 0.445279 | 8.2:+6812289 | MS.gene051837:CDS |
| GAGGTCCAGTAAGACGGTTA+TGG | 0.447310 | 8.2:+6812288 | MS.gene051837:CDS |
| TTTGATTAAACAAGAGTTTG+AGG | 0.451818 | 8.2:+6812661 | MS.gene051837:CDS |
| CAAGGTGTGGTTGATACTAA+AGG | 0.464115 | 8.2:+6812824 | MS.gene051837:CDS |
| TCCCGGGCGGCAGCGCGTGG+CGG | 0.468360 | 8.2:+6812475 | MS.gene051837:CDS |
| GAGGATAGAGGTGAAGATTG+AGG | 0.471283 | 8.2:+6813126 | MS.gene051837:CDS |
| CTGTTGCTGCTGTTCAAGCT+AGG | 0.480095 | 8.2:+6813278 | MS.gene051837:CDS |
| TCTGGGTTCCCTTTGATGGA+TGG | 0.480100 | 8.2:+6812971 | MS.gene051837:CDS |
| TCAGGCCAAGTAATAAACTT+TGG | 0.480951 | 8.2:-6812623 | None:intergenic |
| AGGCTGGTTTCAAAGAGGTT+CGG | 0.481630 | 8.2:+6812533 | MS.gene051837:CDS |
| GGTGGTCTTGTTTGCAGAAG+AGG | 0.482578 | 8.2:+6813107 | MS.gene051837:CDS |
| TTGATTAAACAAGAGTTTGA+GGG | 0.485947 | 8.2:+6812662 | MS.gene051837:CDS |
| CATTGCAGTTGTTGTTGTTG+GGG | 0.491772 | 8.2:-6812049 | None:intergenic |
| ACCGCAACTTCTTCAGCAAT+GGG | 0.492992 | 8.2:+6812266 | MS.gene051837:CDS |
| ATCACAATCACAGTTACAAT+GGG | 0.511273 | 8.2:+6812016 | MS.gene051837:CDS |
| TGAAAGAACGTCCAAAAGAC+TGG | 0.511704 | 8.2:+6812312 | MS.gene051837:CDS |
| ACCAAAATTCCATCCATCAA+AGG | 0.513056 | 8.2:-6812980 | None:intergenic |
| TGGGAATAATCTTCAAAAGA+AGG | 0.515198 | 8.2:-6811930 | None:intergenic |
| GGATATTCAGAAGCTGTGTA+AGG | 0.523114 | 8.2:+6813060 | MS.gene051837:CDS |
| CTCTGTTACTGTTCAAGGTG+TGG | 0.523511 | 8.2:+6812811 | MS.gene051837:CDS |
| CCAAAATTCCATCCATCAAA+GGG | 0.523543 | 8.2:-6812979 | None:intergenic |
| TTGTTTGCAGAAGAGGATAG+AGG | 0.524424 | 8.2:+6813114 | MS.gene051837:CDS |
| CACCGCAACTTCTTCAGCAA+TGG | 0.525112 | 8.2:+6812265 | MS.gene051837:CDS |
| CATCTCCTGAACTCTTCCTC+CGG | 0.527345 | 8.2:-6812362 | None:intergenic |
| TTTGGAATGCCAAATGTTGG+TGG | 0.527866 | 8.2:+6812689 | MS.gene051837:CDS |
| TGAAATGAGAAATGAGAAGA+TGG | 0.532464 | 8.2:+6813195 | MS.gene051837:CDS |
| CATCACAATCACAGTTACAA+TGG | 0.541606 | 8.2:+6812015 | MS.gene051837:CDS |
| TATATCCACCTGTCATAAGC+TGG | 0.547796 | 8.2:+6812562 | MS.gene051837:CDS |
| GTTGTTGGTATTTGTTGTTG+TGG | 0.556247 | 8.2:-6812122 | None:intergenic |
| AAAAGTTCAAAATTCCATGC+AGG | 0.556616 | 8.2:-6813220 | None:intergenic |
| TGCTAAGGGATGCAATTCCC+GGG | 0.559385 | 8.2:+6812459 | MS.gene051837:CDS |
| CTCGAGAACCAGCTTATGAC+AGG | 0.564326 | 8.2:-6812570 | None:intergenic |
| AATTCCCGGGCGGCAGCGCG+TGG | 0.564819 | 8.2:+6812472 | MS.gene051837:CDS |
| ATGTATCAGAGAGCTATGAC+AGG | 0.567243 | 8.2:+6812917 | MS.gene051837:CDS |
| GATGAAATGGTTGCTGAGAA+TGG | 0.571501 | 8.2:+6813247 | MS.gene051837:CDS |
| CTTTAAGGCTGGTTTCAAAG+AGG | 0.577123 | 8.2:+6812528 | MS.gene051837:CDS |
| CTGTCATAAGCTGGTTCTCG+AGG | 0.578653 | 8.2:+6812571 | MS.gene051837:CDS |
| ACAGCTTGTGCGTCGCGACG+CGG | 0.580368 | 8.2:-6812236 | None:intergenic |
| ATGAGAAGATGGATCCTGCA+TGG | 0.580887 | 8.2:+6813206 | MS.gene051837:CDS |
| TGATCATCATTATAAGAACC+AGG | 0.582500 | 8.2:-6812875 | None:intergenic |
| TGTTGCTGCTGTTCAAGCTA+GGG | 0.584860 | 8.2:+6813279 | MS.gene051837:CDS |
| TCCCATTGCTGAAGAAGTTG+CGG | 0.584987 | 8.2:-6812267 | None:intergenic |
| GATGCGTTTGCAAAGGTGAA+AGG | 0.587098 | 8.2:+6813082 | MS.gene051837:CDS |
| ACAATCACCAACAACAACAA+TGG | 0.587762 | 8.2:+6812151 | MS.gene051837:CDS |
| ATACACATCAGAATTTGACT+TGG | 0.591529 | 8.2:+6813008 | MS.gene051837:CDS |
| AACCAGCCTTAAAGGATCAC+CGG | 0.593167 | 8.2:-6812519 | None:intergenic |
| TTATTGCATCGTGGTCGTGT+TGG | 0.605444 | 8.2:+6813319 | MS.gene051837:CDS |
| ATTGAAATAAGAATTCACAT+TGG | 0.606107 | 8.2:-6812753 | None:intergenic |
| AATAATCTTCAAAAGAAGGA+GGG | 0.606158 | 8.2:-6811926 | None:intergenic |
| CAATGGGAGGTCCAGTAAGA+CGG | 0.606401 | 8.2:+6812282 | MS.gene051837:CDS |
| GCACGCGTTTAATGAGAAAG+TGG | 0.606857 | 8.2:+6813036 | MS.gene051837:CDS |
| GTGAAGATTGAGGAGTTGCC+TGG | 0.615794 | 8.2:+6813136 | MS.gene051837:CDS |
| GAATAATCTTCAAAAGAAGG+AGG | 0.616959 | 8.2:-6811927 | None:intergenic |
| GCTCATGATTTATTGCATCG+TGG | 0.641202 | 8.2:+6813310 | MS.gene051837:CDS |
| TGCAATTATTGGAATATGTG+TGG | 0.641263 | 8.2:-6812723 | None:intergenic |
| TGTATCTGGAGACTTGCCAC+CGG | 0.646276 | 8.2:+6812500 | MS.gene051837:CDS |
| CGTTTGCAAAGGTGAAAGGA+AGG | 0.647177 | 8.2:+6813086 | MS.gene051837:CDS |
| CATTGCTGAAGAAGTTGCGG+TGG | 0.654316 | 8.2:-6812264 | None:intergenic |
| TCAATAAGAGGCAGACTCAG+AGG | 0.654570 | 8.2:+6812771 | MS.gene051837:CDS |
| CGCGTTTAATGAGAAAGTGG+AGG | 0.659194 | 8.2:+6813039 | MS.gene051837:CDS |
| GAGAACCAGCTTATGACAGG+TGG | 0.660646 | 8.2:-6812567 | None:intergenic |
| GTGTAAGGATGCGTTTGCAA+AGG | 0.672985 | 8.2:+6813075 | MS.gene051837:CDS |
| GATCATCATTATAAGAACCA+GGG | 0.676277 | 8.2:-6812874 | None:intergenic |
| TTGCAAAGGTGAAAGGAAGG+TGG | 0.690826 | 8.2:+6813089 | MS.gene051837:CDS |
| GCAACTTCTTCAGCAATGGG+AGG | 0.707088 | 8.2:+6812269 | MS.gene051837:CDS |
| AAGAACGTCCAAAAGACTGG+TGG | 0.708336 | 8.2:+6812315 | MS.gene051837:CDS |
| CAGCCTTAAAGGATCACCGG+TGG | 0.709341 | 8.2:-6812516 | None:intergenic |
| TGTATCAGAGAGCTATGACA+GGG | 0.718997 | 8.2:+6812918 | MS.gene051837:CDS |
| CAGCAGTCCAGAATTTCCGG+AGG | 0.724581 | 8.2:+6812346 | MS.gene051837:CDS |
| TAAGGGATGCAATTCCCGGG+CGG | 0.733518 | 8.2:+6812462 | MS.gene051837:CDS |
| GTGATTGTGATGAATCTTCG+GGG | 0.764964 | 8.2:-6812004 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGAAATAAGAATTCACAT+TGG | - | chr8.2:6812756-6812775 | None:intergenic | 20.0% |
| !!! | AAATTCATCTTATTTTCATC+AGG | - | chr8.2:6812644-6812663 | None:intergenic | 20.0% |
| !!! | TGAATTCTTATTTCAATAAG+AGG | + | chr8.2:6812759-6812778 | MS.gene051837:CDS | 20.0% |
| ! | AAAGAAAAACACTTTGCTAA+GGG | + | chr8.2:6812445-6812464 | MS.gene051837:CDS | 25.0% |
| ! | AATAATCTTCAAAAGAAGGA+GGG | - | chr8.2:6811929-6811948 | None:intergenic | 25.0% |
| ! | TTATGCCAAAGTTTATTACT+TGG | + | chr8.2:6812618-6812637 | MS.gene051837:CDS | 25.0% |
| ! | TTGATTAAACAAGAGTTTGA+GGG | + | chr8.2:6812662-6812681 | MS.gene051837:CDS | 25.0% |
| ! | TTTGATTAAACAAGAGTTTG+AGG | + | chr8.2:6812661-6812680 | MS.gene051837:CDS | 25.0% |
| !! | ATTTTCACTGATGTTTTTCT+TGG | + | chr8.2:6812848-6812867 | MS.gene051837:CDS | 25.0% |
| !! | TTATAATGATGATCAGGTTT+TGG | + | chr8.2:6812883-6812902 | MS.gene051837:CDS | 25.0% |
| !!! | TGAACTTTTTGATGATGAAA+TGG | + | chr8.2:6813234-6813253 | MS.gene051837:CDS | 25.0% |
| AAAAGTTCAAAATTCCATGC+AGG | - | chr8.2:6813223-6813242 | None:intergenic | 30.0% | |
| AGGGAATTTGAAAGATTCTT+GGG | + | chr8.2:6812937-6812956 | MS.gene051837:CDS | 30.0% | |
| ATACACATCAGAATTTGACT+TGG | + | chr8.2:6813008-6813027 | MS.gene051837:CDS | 30.0% | |
| ATCAAAATCAATCTCAAAGC+TGG | - | chr8.2:6812183-6812202 | None:intergenic | 30.0% | |
| ATCACAATCACAGTTACAAT+GGG | + | chr8.2:6812016-6812035 | MS.gene051837:CDS | 30.0% | |
| CAAAGAAAAACACTTTGCTA+AGG | + | chr8.2:6812444-6812463 | MS.gene051837:CDS | 30.0% | |
| GAATAATCTTCAAAAGAAGG+AGG | - | chr8.2:6811930-6811949 | None:intergenic | 30.0% | |
| GATCATCATTATAAGAACCA+GGG | - | chr8.2:6812877-6812896 | None:intergenic | 30.0% | |
| GTCAAATTCTGATGTGTATA+AGG | - | chr8.2:6813007-6813026 | None:intergenic | 30.0% | |
| TAAGAATTCACATTGGACTT+AGG | - | chr8.2:6812749-6812768 | None:intergenic | 30.0% | |
| TGAAATGAGAAATGAGAAGA+TGG | + | chr8.2:6813195-6813214 | MS.gene051837:CDS | 30.0% | |
| TGATCATCATTATAAGAACC+AGG | - | chr8.2:6812878-6812897 | None:intergenic | 30.0% | |
| TGCAATTATTGGAATATGTG+TGG | - | chr8.2:6812726-6812745 | None:intergenic | 30.0% | |
| TGGGAATAATCTTCAAAAGA+AGG | - | chr8.2:6811933-6811952 | None:intergenic | 30.0% | |
| TGGTTCTTATAATGATGATC+AGG | + | chr8.2:6812877-6812896 | MS.gene051837:CDS | 30.0% | |
| TTCCATTTCAGGGAAAAAAT+GGG | - | chr8.2:6811952-6811971 | None:intergenic | 30.0% | |
| !! | CAAATCGAATGAGAGTATTA+AGG | - | chr8.2:6812097-6812116 | None:intergenic | 30.0% |
| !! | CTTTTCTTTGGGTTTACATT+TGG | - | chr8.2:6811978-6811997 | None:intergenic | 30.0% |
| ACAATCACCAACAACAACAA+TGG | + | chr8.2:6812151-6812170 | MS.gene051837:CDS | 35.0% | |
| ACATTTGGTTGTTCCATTTC+AGG | - | chr8.2:6811963-6811982 | None:intergenic | 35.0% | |
| ACCAAAATTCCATCCATCAA+AGG | - | chr8.2:6812983-6813002 | None:intergenic | 35.0% | |
| CAGGGAATTTGAAAGATTCT+TGG | + | chr8.2:6812936-6812955 | MS.gene051837:CDS | 35.0% | |
| CATCACAATCACAGTTACAA+TGG | + | chr8.2:6812015-6812034 | MS.gene051837:CDS | 35.0% | |
| CATTTGGTTGTTCCATTTCA+GGG | - | chr8.2:6811962-6811981 | None:intergenic | 35.0% | |
| CCAAAATTCCATCCATCAAA+GGG | - | chr8.2:6812982-6813001 | None:intergenic | 35.0% | |
| CTGTGATTGTGATGAATCTT+CGG | - | chr8.2:6812009-6812028 | None:intergenic | 35.0% | |
| GTTCCATTTCAGGGAAAAAA+TGG | - | chr8.2:6811953-6811972 | None:intergenic | 35.0% | |
| TACATTGAACCACCAACATT+TGG | - | chr8.2:6812701-6812720 | None:intergenic | 35.0% | |
| TCAGGCCAAGTAATAAACTT+TGG | - | chr8.2:6812626-6812645 | None:intergenic | 35.0% | |
| TGTGATTGTGATGAATCTTC+GGG | - | chr8.2:6812008-6812027 | None:intergenic | 35.0% | |
| TTGGACTTAGGTGCAATTAT+TGG | - | chr8.2:6812737-6812756 | None:intergenic | 35.0% | |
| ! | AAACAGCTGATTCAAGTTCT+TGG | - | chr8.2:6812424-6812443 | None:intergenic | 35.0% |
| ! | AAGGTGTGGTTGATACTAAA+GGG | + | chr8.2:6812825-6812844 | MS.gene051837:CDS | 35.0% |
| ! | TAACAGAGTATGCAGTTTTC+TGG | - | chr8.2:6812799-6812818 | None:intergenic | 35.0% |
| ! | TCATTGCAGTTGTTGTTGTT+GGG | - | chr8.2:6812053-6812072 | None:intergenic | 35.0% |
| ! | TTGAAAGATTCTTGGGTTGT+TGG | + | chr8.2:6812944-6812963 | MS.gene051837:CDS | 35.0% |
| !! | GTTGTTGGTATTTGTTGTTG+TGG | - | chr8.2:6812125-6812144 | None:intergenic | 35.0% |
| !!! | CTTTTTGGAATGCCAAATGT+TGG | + | chr8.2:6812686-6812705 | MS.gene051837:CDS | 35.0% |
| !!! | TTCCCATTTTTTCCCTGAAA+TGG | + | chr8.2:6811947-6811966 | MS.gene051837:CDS | 35.0% |
| AAATTCTGGGTTCCCTTTGA+TGG | + | chr8.2:6812967-6812986 | MS.gene051837:CDS | 40.0% | |
| AAGAGTTCAGGAGATGTTTC+CGG | + | chr8.2:6812369-6812388 | MS.gene051837:CDS | 40.0% | |
| ATGTATCAGAGAGCTATGAC+AGG | + | chr8.2:6812917-6812936 | MS.gene051837:CDS | 40.0% | |
| GATGAAATGGTTGCTGAGAA+TGG | + | chr8.2:6813247-6813266 | MS.gene051837:CDS | 40.0% | |
| GCATACTCTGTTACTGTTCA+AGG | + | chr8.2:6812806-6812825 | MS.gene051837:CDS | 40.0% | |
| GCTCATGATTTATTGCATCG+TGG | + | chr8.2:6813310-6813329 | MS.gene051837:CDS | 40.0% | |
| GGATATTCAGAAGCTGTGTA+AGG | + | chr8.2:6813060-6813079 | MS.gene051837:CDS | 40.0% | |
| GTGATTGTGATGAATCTTCG+GGG | - | chr8.2:6812007-6812026 | None:intergenic | 40.0% | |
| TATATCCACCTGTCATAAGC+TGG | + | chr8.2:6812562-6812581 | MS.gene051837:CDS | 40.0% | |
| TGAAAGAACGTCCAAAAGAC+TGG | + | chr8.2:6812312-6812331 | MS.gene051837:CDS | 40.0% | |
| TGTATCAGAGAGCTATGACA+GGG | + | chr8.2:6812918-6812937 | MS.gene051837:CDS | 40.0% | |
| TTGTTTGCAGAAGAGGATAG+AGG | + | chr8.2:6813114-6813133 | MS.gene051837:CDS | 40.0% | |
| ! | CAAGAGTTTGAGGGTCTTTT+TGG | + | chr8.2:6812671-6812690 | MS.gene051837:CDS | 40.0% |
| ! | CAAGGTGTGGTTGATACTAA+AGG | + | chr8.2:6812824-6812843 | MS.gene051837:CDS | 40.0% |
| ! | CATTGCAGTTGTTGTTGTTG+GGG | - | chr8.2:6812052-6812071 | None:intergenic | 40.0% |
| ! | CGGGGTGATTTCTTTTCTTT+GGG | - | chr8.2:6811989-6812008 | None:intergenic | 40.0% |
| ! | CTTGGGTTGTTGGAAATTCT+GGG | + | chr8.2:6812954-6812973 | MS.gene051837:CDS | 40.0% |
| ! | GAGTTGTCCATTGTTGTTGT+TGG | - | chr8.2:6812161-6812180 | None:intergenic | 40.0% |
| ! | GATGTTTTTCTTGGTTACCC+TGG | + | chr8.2:6812857-6812876 | MS.gene051837:CDS | 40.0% |
| ! | GGTGATTGTTGTTGTGTTGT+TGG | - | chr8.2:6812140-6812159 | None:intergenic | 40.0% |
| ! | GTCATTGCAGTTGTTGTTGT+TGG | - | chr8.2:6812054-6812073 | None:intergenic | 40.0% |
| ! | GTTTCAAAGAGGTTCGGTTT+GGG | + | chr8.2:6812539-6812558 | MS.gene051837:CDS | 40.0% |
| ! | TCGGGGTGATTTCTTTTCTT+TGG | - | chr8.2:6811990-6812009 | None:intergenic | 40.0% |
| ! | TCTTGGGTTGTTGGAAATTC+TGG | + | chr8.2:6812953-6812972 | MS.gene051837:CDS | 40.0% |
| ! | TTTGGAATGCCAAATGTTGG+TGG | + | chr8.2:6812689-6812708 | MS.gene051837:CDS | 40.0% |
| !! | CCCTTTGATGGATGGAATTT+TGG | + | chr8.2:6812979-6812998 | MS.gene051837:CDS | 40.0% |
| !! | CTCTTTGAAACCAGCCTTAA+AGG | - | chr8.2:6812530-6812549 | None:intergenic | 40.0% |
| !! | CTTTAAGGCTGGTTTCAAAG+AGG | + | chr8.2:6812528-6812547 | MS.gene051837:CDS | 40.0% |
| AACCAGCCTTAAAGGATCAC+CGG | - | chr8.2:6812522-6812541 | None:intergenic | 45.0% | |
| AACTCTTCCTCCGGAAATTC+TGG | - | chr8.2:6812356-6812375 | None:intergenic | 45.0% | |
| AAGAACGTCCAAAAGACTGG+TGG | + | chr8.2:6812315-6812334 | MS.gene051837:CDS | 45.0% | |
| AATTTCCGGAGGAAGAGTTC+AGG | + | chr8.2:6812357-6812376 | MS.gene051837:CDS | 45.0% | |
| ACCGCAACTTCTTCAGCAAT+GGG | + | chr8.2:6812266-6812285 | MS.gene051837:CDS | 45.0% | |
| AGGTCCAGTAAGACGGTTAT+GGG | + | chr8.2:6812289-6812308 | MS.gene051837:CDS | 45.0% | |
| ATGAGAAGATGGATCCTGCA+TGG | + | chr8.2:6813206-6813225 | MS.gene051837:CDS | 45.0% | |
| ATGCAGCAGTCCAGAATTTC+CGG | + | chr8.2:6812343-6812362 | MS.gene051837:CDS | 45.0% | |
| CGCGTTTAATGAGAAAGTGG+AGG | + | chr8.2:6813039-6813058 | MS.gene051837:CDS | 45.0% | |
| CGTTTGCAAAGGTGAAAGGA+AGG | + | chr8.2:6813086-6813105 | MS.gene051837:CDS | 45.0% | |
| CTCTGTTACTGTTCAAGGTG+TGG | + | chr8.2:6812811-6812830 | MS.gene051837:CDS | 45.0% | |
| GAGGATAGAGGTGAAGATTG+AGG | + | chr8.2:6813126-6813145 | MS.gene051837:CDS | 45.0% | |
| GATGCGTTTGCAAAGGTGAA+AGG | + | chr8.2:6813082-6813101 | MS.gene051837:CDS | 45.0% | |
| GCACGCGTTTAATGAGAAAG+TGG | + | chr8.2:6813036-6813055 | MS.gene051837:CDS | 45.0% | |
| GTGTAAGGATGCGTTTGCAA+AGG | + | chr8.2:6813075-6813094 | MS.gene051837:CDS | 45.0% | |
| TCAATAAGAGGCAGACTCAG+AGG | + | chr8.2:6812771-6812790 | MS.gene051837:CDS | 45.0% | |
| TCCCATTGCTGAAGAAGTTG+CGG | - | chr8.2:6812270-6812289 | None:intergenic | 45.0% | |
| TGTTGCTGCTGTTCAAGCTA+GGG | + | chr8.2:6813279-6813298 | MS.gene051837:CDS | 45.0% | |
| TTATTGCATCGTGGTCGTGT+TGG | + | chr8.2:6813319-6813338 | MS.gene051837:CDS | 45.0% | |
| TTCACCCATAACCGTCTTAC+TGG | - | chr8.2:6812296-6812315 | None:intergenic | 45.0% | |
| TTGCAAAGGTGAAAGGAAGG+TGG | + | chr8.2:6813089-6813108 | MS.gene051837:CDS | 45.0% | |
| TTGCTAAGGGATGCAATTCC+CGG | + | chr8.2:6812458-6812477 | MS.gene051837:CDS | 45.0% | |
| ! | CATTTCTGCCACCAGTCTTT+TGG | - | chr8.2:6812326-6812345 | None:intergenic | 45.0% |
| ! | GGTTTCAAAGAGGTTCGGTT+TGG | + | chr8.2:6812538-6812557 | MS.gene051837:CDS | 45.0% |
| !! | AGGCTGGTTTCAAAGAGGTT+CGG | + | chr8.2:6812533-6812552 | MS.gene051837:CDS | 45.0% |
| CAATGGGAGGTCCAGTAAGA+CGG | + | chr8.2:6812282-6812301 | MS.gene051837:CDS | 50.0% | |
| CACCGCAACTTCTTCAGCAA+TGG | + | chr8.2:6812265-6812284 | MS.gene051837:CDS | 50.0% | |
| CATCTCCTGAACTCTTCCTC+CGG | - | chr8.2:6812365-6812384 | None:intergenic | 50.0% | |
| CATTGCTGAAGAAGTTGCGG+TGG | - | chr8.2:6812267-6812286 | None:intergenic | 50.0% | |
| CTCGAGAACCAGCTTATGAC+AGG | - | chr8.2:6812573-6812592 | None:intergenic | 50.0% | |
| CTGTTGCTGCTGTTCAAGCT+AGG | + | chr8.2:6813278-6813297 | MS.gene051837:CDS | 50.0% | |
| GAGAACCAGCTTATGACAGG+TGG | - | chr8.2:6812570-6812589 | None:intergenic | 50.0% | |
| GAGGTCCAGTAAGACGGTTA+TGG | + | chr8.2:6812288-6812307 | MS.gene051837:CDS | 50.0% | |
| GCAACTTCTTCAGCAATGGG+AGG | + | chr8.2:6812269-6812288 | MS.gene051837:CDS | 50.0% | |
| GTGAAGATTGAGGAGTTGCC+TGG | + | chr8.2:6813136-6813155 | MS.gene051837:CDS | 50.0% | |
| TCTGGGTTCCCTTTGATGGA+TGG | + | chr8.2:6812971-6812990 | MS.gene051837:CDS | 50.0% | |
| TGCTAAGGGATGCAATTCCC+GGG | + | chr8.2:6812459-6812478 | MS.gene051837:CDS | 50.0% | |
| TGTATCTGGAGACTTGCCAC+CGG | + | chr8.2:6812500-6812519 | MS.gene051837:CDS | 50.0% | |
| TTGCCACCGGTGATCCTTTA+AGG | + | chr8.2:6812513-6812532 | MS.gene051837:CDS | 50.0% | |
| ! | CGAACGTTGCTTTGCTCATC+CGG | - | chr8.2:6812391-6812410 | None:intergenic | 50.0% |
| ! | CTGTCATAAGCTGGTTCTCG+AGG | + | chr8.2:6812571-6812590 | MS.gene051837:CDS | 50.0% |
| !! | GGTGGTCTTGTTTGCAGAAG+AGG | + | chr8.2:6813107-6813126 | MS.gene051837:CDS | 50.0% |
| CAGCAAGCACCAAGAACACC+AGG | - | chr8.2:6813157-6813176 | None:intergenic | 55.0% | |
| CAGCAGTCCAGAATTTCCGG+AGG | + | chr8.2:6812346-6812365 | MS.gene051837:CDS | 55.0% | |
| CAGCCTTAAAGGATCACCGG+TGG | - | chr8.2:6812519-6812538 | None:intergenic | 55.0% | |
| GAGGAGTTGCCTGGTGTTCT+TGG | + | chr8.2:6813145-6813164 | MS.gene051837:CDS | 55.0% | |
| TAAGGGATGCAATTCCCGGG+CGG | + | chr8.2:6812462-6812481 | MS.gene051837:CDS | 55.0% | |
| !! | CACCGGTGATCCTTTAAGGC+TGG | + | chr8.2:6812517-6812536 | MS.gene051837:CDS | 55.0% |
| AGCGCGTGGCGGTTTGTATC+TGG | + | chr8.2:6812486-6812505 | MS.gene051837:CDS | 60.0% | |
| ACAGCTTGTGCGTCGCGACG+CGG | - | chr8.2:6812239-6812258 | None:intergenic | 65.0% | |
| AATTCCCGGGCGGCAGCGCG+TGG | + | chr8.2:6812472-6812491 | MS.gene051837:CDS | 75.0% | |
| ! | AACCGCCACGCGCTGCCGCC+CGG | - | chr8.2:6812480-6812499 | None:intergenic | 80.0% |
| ! | ACCGCCACGCGCTGCCGCCC+GGG | - | chr8.2:6812479-6812498 | None:intergenic | 85.0% |
| ! | TCCCGGGCGGCAGCGCGTGG+CGG | + | chr8.2:6812475-6812494 | MS.gene051837:CDS | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 6811903 | 6813354 | 6811903 | ID=MS.gene051837 |
| chr8.2 | mRNA | 6811903 | 6813354 | 6811903 | ID=MS.gene051837.t1;Parent=MS.gene051837 |
| chr8.2 | exon | 6811903 | 6813354 | 6811903 | ID=MS.gene051837.t1.exon1;Parent=MS.gene051837.t1 |
| chr8.2 | CDS | 6811903 | 6813354 | 6811903 | ID=cds.MS.gene051837.t1;Parent=MS.gene051837.t1 |
| Gene Sequence |
| Protein sequence |
