Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20675.t1 | XP_003611549.2 | 92.2 | 116 | 4 | 2 | 1 | 111 | 1 | 116 | 1.40E-47 | 198.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20675.t1 | G7K2F6 | 92.2 | 116 | 4 | 2 | 1 | 111 | 1 | 116 | 9.9e-48 | 198.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055961 | MS.gene20675 | 0.806997 | 6.27E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20675.t1 | MTR_5g015190 | 91.176 | 102 | 4 | 2 | 1 | 97 | 1 | 102 | 3.55e-60 | 180 |
| MS.gene20675.t1 | MTR_4g097350 | 54.839 | 62 | 27 | 1 | 9 | 69 | 32 | 93 | 1.92e-14 | 66.2 |
| MS.gene20675.t1 | MTR_4g097280 | 54.839 | 62 | 27 | 1 | 9 | 69 | 31 | 92 | 2.70e-13 | 63.2 |
| MS.gene20675.t1 | MTR_4g097360 | 56.364 | 55 | 23 | 1 | 16 | 69 | 35 | 89 | 1.92e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20675.t1 | AT2G22880 | 39.091 | 110 | 60 | 4 | 1 | 110 | 1 | 103 | 1.55e-12 | 59.3 |
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACCCTAACTGCTGTTAA+AGG | 0.195754 | 5.4:+8904987 | None:intergenic |
| CAACAATTTGTTAGTGATAA+AGG | 0.212595 | 5.4:-8904888 | MS.gene20675:CDS |
| GACAAAGCAATCAGGTTTAT+TGG | 0.268685 | 5.4:-8904838 | MS.gene20675:CDS |
| TGTTGTTGAGGCTTGAATTC+TGG | 0.279950 | 5.4:+8904912 | None:intergenic |
| AACAATTTGTTAGTGATAAA+GGG | 0.288388 | 5.4:-8904887 | MS.gene20675:CDS |
| CACAACCTTTAACAGCAGTT+AGG | 0.395073 | 5.4:-8904992 | MS.gene20675:CDS |
| CTTCACCATCTTCTCAGAGT+TGG | 0.407398 | 5.4:-8904803 | MS.gene20675:CDS |
| CACCATCTTCTCAGAGTTGG+TGG | 0.431356 | 5.4:-8904800 | MS.gene20675:CDS |
| ATTATGAGACTTAGGATAAG+AGG | 0.439722 | 5.4:+8905079 | None:intergenic |
| AATGAATTATTATGAGACTT+AGG | 0.448016 | 5.4:+8905071 | None:intergenic |
| ACAACCTTTAACAGCAGTTA+GGG | 0.458936 | 5.4:-8904991 | MS.gene20675:CDS |
| AGTGGTGTGACAAAGCAATC+AGG | 0.507552 | 5.4:-8904846 | MS.gene20675:CDS |
| CTAACTGCTGTTAAAGGTTG+TGG | 0.514313 | 5.4:+8904993 | None:intergenic |
| GACCACCAACTCTGAGAAGA+TGG | 0.520594 | 5.4:+8904798 | None:intergenic |
| ATTGTGGCTTTCTAACGGAA+TGG | 0.529161 | 5.4:+8905045 | None:intergenic |
| AGACTTAGGATAAGAGGTGG+TGG | 0.557065 | 5.4:+8905085 | None:intergenic |
| GGTGGTGGAATAAGCTTCCA+TGG | 0.561828 | 5.4:+8905100 | None:intergenic |
| ACAATTTGTTAGTGATAAAG+GGG | 0.570288 | 5.4:-8904886 | MS.gene20675:CDS |
| ACAAATTGTTGATGTTGTTG+AGG | 0.598417 | 5.4:+8904900 | None:intergenic |
| GAAAGCCACAATCAAAACCA+TGG | 0.621885 | 5.4:-8905034 | MS.gene20675:CDS |
| GAGTTAGTTCAACAACTCAC+AGG | 0.631511 | 5.4:-8904939 | MS.gene20675:CDS |
| GTGCAATATCAAGAGAGTAG+TGG | 0.659499 | 5.4:-8904864 | MS.gene20675:CDS |
| ATGAGACTTAGGATAAGAGG+TGG | 0.667730 | 5.4:+8905082 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAATTATTATGAGACTT+AGG | + | chr5.4:8904815-8904834 | None:intergenic | 20.0% |
| !!! | AACAATTTGTTAGTGATAAA+GGG | - | chr5.4:8904996-8905015 | MS.gene20675:CDS | 20.0% |
| !!! | ACAATTTGTTAGTGATAAAG+GGG | - | chr5.4:8904997-8905016 | MS.gene20675:CDS | 25.0% |
| !!! | CAACAATTTGTTAGTGATAA+AGG | - | chr5.4:8904995-8905014 | MS.gene20675:CDS | 25.0% |
| ACAAATTGTTGATGTTGTTG+AGG | + | chr5.4:8904986-8905005 | None:intergenic | 30.0% | |
| ATTATGAGACTTAGGATAAG+AGG | + | chr5.4:8904807-8904826 | None:intergenic | 30.0% | |
| !! | TTTTGATTGTGGCTTTCTAA+CGG | + | chr5.4:8904846-8904865 | None:intergenic | 30.0% |
| !!! | TTTTTCCATGGTTTTGATTG+TGG | + | chr5.4:8904857-8904876 | None:intergenic | 30.0% |
| ACAACCTTTAACAGCAGTTA+GGG | - | chr5.4:8904892-8904911 | MS.gene20675:CDS | 35.0% | |
| GACAAAGCAATCAGGTTTAT+TGG | - | chr5.4:8905045-8905064 | MS.gene20675:CDS | 35.0% | |
| TAAACCCTAACTGCTGTTAA+AGG | + | chr5.4:8904899-8904918 | None:intergenic | 35.0% | |
| ACCATGGAAAAAAGCTTCAG+TGG | - | chr5.4:8904865-8904884 | MS.gene20675:CDS | 40.0% | |
| ATGAGACTTAGGATAAGAGG+TGG | + | chr5.4:8904804-8904823 | None:intergenic | 40.0% | |
| ATTGTGGCTTTCTAACGGAA+TGG | + | chr5.4:8904841-8904860 | None:intergenic | 40.0% | |
| CACAACCTTTAACAGCAGTT+AGG | - | chr5.4:8904891-8904910 | MS.gene20675:CDS | 40.0% | |
| CTAACTGCTGTTAAAGGTTG+TGG | + | chr5.4:8904893-8904912 | None:intergenic | 40.0% | |
| GAAAGCCACAATCAAAACCA+TGG | - | chr5.4:8904849-8904868 | MS.gene20675:CDS | 40.0% | |
| GAGTTAGTTCAACAACTCAC+AGG | - | chr5.4:8904944-8904963 | MS.gene20675:CDS | 40.0% | |
| GTGCAATATCAAGAGAGTAG+TGG | - | chr5.4:8905019-8905038 | MS.gene20675:CDS | 40.0% | |
| TGTTGTTGAGGCTTGAATTC+TGG | + | chr5.4:8904974-8904993 | None:intergenic | 40.0% | |
| AGACTTAGGATAAGAGGTGG+TGG | + | chr5.4:8904801-8904820 | None:intergenic | 45.0% | |
| AGTGGTGTGACAAAGCAATC+AGG | - | chr5.4:8905037-8905056 | MS.gene20675:CDS | 45.0% | |
| CTTCACCATCTTCTCAGAGT+TGG | - | chr5.4:8905080-8905099 | MS.gene20675:CDS | 45.0% | |
| !!! | GCCACTGAAGCTTTTTTCCA+TGG | + | chr5.4:8904869-8904888 | None:intergenic | 45.0% |
| CACCATCTTCTCAGAGTTGG+TGG | - | chr5.4:8905083-8905102 | MS.gene20675:CDS | 50.0% | |
| ! | GACCACCAACTCTGAGAAGA+TGG | + | chr5.4:8905088-8905107 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 8904785 | 8905120 | 8904785 | ID=MS.gene20675 |
| chr5.4 | mRNA | 8904785 | 8905120 | 8904785 | ID=MS.gene20675.t1;Parent=MS.gene20675 |
| chr5.4 | exon | 8904785 | 8905120 | 8904785 | ID=MS.gene20675.t1.exon1;Parent=MS.gene20675.t1 |
| chr5.4 | CDS | 8904785 | 8905120 | 8904785 | ID=cds.MS.gene20675.t1;Parent=MS.gene20675.t1 |
| Gene Sequence |
| Protein sequence |
