Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20895.t1 | XP_003591331.1 | 97.5 | 121 | 3 | 0 | 1 | 121 | 1 | 121 | 5.30E-61 | 243.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20895.t1 | Q8S925 | 69.6 | 112 | 34 | 0 | 7 | 118 | 8 | 119 | 1.8e-41 | 169.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20895.t1 | G7IBT1 | 97.5 | 121 | 3 | 0 | 1 | 121 | 1 | 121 | 3.8e-61 | 243.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051982 | MS.gene20895 | 0.812146 | 4.91E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20895.t1 | MTR_1g086310 | 97.521 | 121 | 3 | 0 | 1 | 121 | 1 | 121 | 9.28e-86 | 245 |
| MS.gene20895.t1 | MTR_4g123760 | 68.644 | 118 | 37 | 0 | 1 | 118 | 1 | 118 | 2.78e-59 | 178 |
| MS.gene20895.t1 | MTR_2g023430 | 54.310 | 116 | 53 | 0 | 5 | 120 | 4 | 119 | 1.93e-43 | 138 |
| MS.gene20895.t1 | MTR_4g048510 | 54.310 | 116 | 53 | 0 | 3 | 118 | 2 | 117 | 2.01e-43 | 138 |
| MS.gene20895.t1 | MTR_4g101090 | 52.586 | 116 | 55 | 0 | 5 | 120 | 4 | 119 | 3.07e-41 | 133 |
| MS.gene20895.t1 | MTR_2g088230 | 48.246 | 114 | 47 | 1 | 5 | 118 | 4 | 105 | 6.23e-33 | 111 |
| MS.gene20895.t1 | MTR_7g096540 | 74.194 | 62 | 16 | 0 | 57 | 118 | 1 | 62 | 1.86e-29 | 101 |
| MS.gene20895.t1 | MTR_4g037225 | 45.536 | 112 | 59 | 2 | 6 | 115 | 5 | 116 | 7.87e-27 | 96.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20895.t1 | AT3G06420 | 69.027 | 113 | 35 | 0 | 6 | 118 | 7 | 119 | 8.25e-57 | 172 |
| MS.gene20895.t1 | AT3G15580 | 70.536 | 112 | 33 | 0 | 7 | 118 | 4 | 115 | 1.10e-56 | 172 |
| MS.gene20895.t1 | AT4G21980 | 57.265 | 117 | 50 | 0 | 5 | 121 | 4 | 120 | 4.80e-45 | 142 |
| MS.gene20895.t1 | AT4G21980 | 57.265 | 117 | 50 | 0 | 5 | 121 | 19 | 135 | 1.14e-44 | 142 |
| MS.gene20895.t1 | AT1G62040 | 56.897 | 116 | 50 | 0 | 3 | 118 | 2 | 117 | 2.12e-44 | 140 |
| MS.gene20895.t1 | AT2G45170 | 53.719 | 121 | 56 | 0 | 1 | 121 | 1 | 121 | 3.16e-44 | 140 |
| MS.gene20895.t1 | AT2G45170 | 53.719 | 121 | 56 | 0 | 1 | 121 | 1 | 121 | 3.16e-44 | 140 |
| MS.gene20895.t1 | AT4G04620 | 53.448 | 116 | 54 | 0 | 5 | 120 | 4 | 119 | 2.63e-42 | 135 |
| MS.gene20895.t1 | AT4G04620 | 53.448 | 116 | 54 | 0 | 5 | 120 | 4 | 119 | 2.63e-42 | 135 |
| MS.gene20895.t1 | AT4G04620 | 53.448 | 116 | 54 | 0 | 5 | 120 | 4 | 119 | 2.63e-42 | 135 |
| MS.gene20895.t1 | AT2G05630 | 53.509 | 114 | 53 | 0 | 5 | 118 | 4 | 117 | 5.94e-42 | 134 |
| MS.gene20895.t1 | AT4G16520 | 54.386 | 114 | 52 | 0 | 5 | 118 | 4 | 117 | 1.41e-41 | 134 |
| MS.gene20895.t1 | AT4G16520 | 54.386 | 114 | 52 | 0 | 5 | 118 | 4 | 117 | 1.41e-41 | 134 |
| MS.gene20895.t1 | AT3G60640 | 52.174 | 115 | 55 | 0 | 6 | 120 | 5 | 119 | 5.11e-41 | 132 |
| MS.gene20895.t1 | AT2G05630 | 52.778 | 108 | 51 | 0 | 11 | 118 | 54 | 161 | 1.34e-38 | 127 |
| MS.gene20895.t1 | AT4G16520 | 55.682 | 88 | 39 | 0 | 31 | 118 | 30 | 117 | 1.02e-30 | 106 |
| MS.gene20895.t1 | AT1G62040 | 52.688 | 93 | 44 | 0 | 3 | 95 | 2 | 94 | 1.55e-29 | 102 |
Find 22 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGATTCGATCGGGGTATT+TGG | 0.168599 | 1.3:-61137168 | None:intergenic |
| TACCAAACGTAAACTCGTTC+TGG | 0.338661 | 1.3:-61137031 | None:intergenic |
| TCCAGCTGGTAAATTAAGTC+TGG | 0.354424 | 1.3:-61137492 | None:intergenic |
| CAAATGCGATCTGCCTCATT+TGG | 0.355422 | 1.3:+61137299 | MS.gene20895:CDS |
| AAGAATACTTTACCTCAAAC+TGG | 0.387404 | 1.3:+61137536 | MS.gene20895:CDS |
| GGACTTACGGGGATTCGATC+GGG | 0.390336 | 1.3:-61137176 | None:intergenic |
| TATAGCACTGAGAAAACCTT+TGG | 0.412532 | 1.3:+61137715 | MS.gene20895:CDS |
| TGCTATTGCAGCTAGTATGA+TGG | 0.424492 | 1.3:+61137642 | MS.gene20895:intron |
| GCAAGTTTATTACCAGTTTG+AGG | 0.425620 | 1.3:-61137548 | None:intergenic |
| CAAATGAGGCAGATCGCATT+TGG | 0.427187 | 1.3:-61137298 | None:intergenic |
| AATAGTTGACATTTATGTAC+AGG | 0.446245 | 1.3:-61137740 | None:intergenic |
| TCCAGACTTAATTTACCAGC+TGG | 0.456823 | 1.3:+61137491 | MS.gene20895:CDS |
| TCTTCTTCTTCAGGTGATTG+TGG | 0.457646 | 1.3:+61137266 | MS.gene20895:intron |
| CTCTGTTTATAGATCATTCA+AGG | 0.464700 | 1.3:+61137666 | MS.gene20895:CDS |
| TTCCAGAACGAGTTTACGTT+TGG | 0.466165 | 1.3:+61137029 | MS.gene20895:CDS |
| GGGACTTACGGGGATTCGAT+CGG | 0.493031 | 1.3:-61137177 | None:intergenic |
| TTATAGATCATTCAAGGATG+AGG | 0.555700 | 1.3:+61137672 | MS.gene20895:CDS |
| AGATCATTCAAGGATGAGGA+TGG | 0.557576 | 1.3:+61137676 | MS.gene20895:CDS |
| CATTTATGTACAGGATCCAA+AGG | 0.566947 | 1.3:-61137731 | None:intergenic |
| GTTCCACGAGACTTGTCAGT+TGG | 0.569472 | 1.3:+61137446 | MS.gene20895:CDS |
| GACTTACGGGGATTCGATCG+GGG | 0.607489 | 1.3:-61137175 | None:intergenic |
| TGTCCAACTGACAAGTCTCG+TGG | 0.654531 | 1.3:-61137449 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAAATTAGATTTAAGATT+AGG | - | chr1.3:61137579-61137598 | None:intergenic | 10.0% |
| !! | AAGCCAAATTGAAATTAAAT+TGG | - | chr1.3:61137228-61137247 | None:intergenic | 20.0% |
| !!! | AATAATTTTTCTTCTTCTTC+AGG | + | chr1.3:61137257-61137276 | MS.gene20895:intron | 20.0% |
| ! | ATACGAAAATCAATGATTGA+TGG | - | chr1.3:61137201-61137220 | None:intergenic | 25.0% |
| ! | CCAAATTGAAATTAAATTGG+AGG | - | chr1.3:61137225-61137244 | None:intergenic | 25.0% |
| ! | TACGAAAATCAATGATTGAT+GGG | - | chr1.3:61137200-61137219 | None:intergenic | 25.0% |
| !! | CCTCCAATTTAATTTCAATT+TGG | + | chr1.3:61137222-61137241 | MS.gene20895:intron | 25.0% |
| AAGAATACTTTACCTCAAAC+TGG | + | chr1.3:61137536-61137555 | MS.gene20895:CDS | 30.0% | |
| CTCTGTTTATAGATCATTCA+AGG | + | chr1.3:61137666-61137685 | MS.gene20895:CDS | 30.0% | |
| TTATAGATCATTCAAGGATG+AGG | + | chr1.3:61137672-61137691 | MS.gene20895:CDS | 30.0% | |
| ! | TTTTCTCAGTGCTATAATAC+AGG | - | chr1.3:61137711-61137730 | None:intergenic | 30.0% |
| !!! | TACTTTTTCTTCTCCAAATG+AGG | - | chr1.3:61137315-61137334 | None:intergenic | 30.0% |
| CAACATGAAAAAGCAACAAG+AGG | - | chr1.3:61137609-61137628 | None:intergenic | 35.0% | |
| GCAAGTTTATTACCAGTTTG+AGG | - | chr1.3:61137551-61137570 | None:intergenic | 35.0% | |
| TCAATGATTGATGGGACTTA+CGG | - | chr1.3:61137192-61137211 | None:intergenic | 35.0% | |
| ! | TATAGCACTGAGAAAACCTT+TGG | + | chr1.3:61137715-61137734 | MS.gene20895:CDS | 35.0% |
| AATGATTGATGGGACTTACG+GGG | - | chr1.3:61137190-61137209 | None:intergenic | 40.0% | |
| AGATCATTCAAGGATGAGGA+TGG | + | chr1.3:61137676-61137695 | MS.gene20895:CDS | 40.0% | |
| CAATGATTGATGGGACTTAC+GGG | - | chr1.3:61137191-61137210 | None:intergenic | 40.0% | |
| TACCAAACGTAAACTCGTTC+TGG | - | chr1.3:61137034-61137053 | None:intergenic | 40.0% | |
| TCCAGACTTAATTTACCAGC+TGG | + | chr1.3:61137491-61137510 | MS.gene20895:CDS | 40.0% | |
| TCCAGCTGGTAAATTAAGTC+TGG | - | chr1.3:61137495-61137514 | None:intergenic | 40.0% | |
| TCTTCTTCTTCAGGTGATTG+TGG | + | chr1.3:61137266-61137285 | MS.gene20895:intron | 40.0% | |
| TGCTATTGCAGCTAGTATGA+TGG | + | chr1.3:61137642-61137661 | MS.gene20895:intron | 40.0% | |
| TTCCAGAACGAGTTTACGTT+TGG | + | chr1.3:61137029-61137048 | MS.gene20895:CDS | 40.0% | |
| ! | TTTTCGCGTGTGTAGAACAA+AGG | + | chr1.3:61137120-61137139 | MS.gene20895:intron | 40.0% |
| CAAATGAGGCAGATCGCATT+TGG | - | chr1.3:61137301-61137320 | None:intergenic | 45.0% | |
| CAAATGCGATCTGCCTCATT+TGG | + | chr1.3:61137299-61137318 | MS.gene20895:CDS | 45.0% | |
| ! | ACAAAGAGAGCTTTTCCAGC+TGG | - | chr1.3:61137509-61137528 | None:intergenic | 45.0% |
| !! | ATAAGATAAAATTATTAAAA+AGG | - | chr1.3:61137072-61137091 | None:intergenic | 5.0% |
| GTTCCACGAGACTTGTCAGT+TGG | + | chr1.3:61137446-61137465 | MS.gene20895:CDS | 50.0% | |
| TGTCCAACTGACAAGTCTCG+TGG | - | chr1.3:61137452-61137471 | None:intergenic | 50.0% | |
| GACTTACGGGGATTCGATCG+GGG | - | chr1.3:61137178-61137197 | None:intergenic | 55.0% | |
| GGACTTACGGGGATTCGATC+GGG | - | chr1.3:61137179-61137198 | None:intergenic | 55.0% | |
| GGGACTTACGGGGATTCGAT+CGG | - | chr1.3:61137180-61137199 | None:intergenic | 55.0% | |
| ! | GGGGATTCGATCGGGGTATT+TGG | - | chr1.3:61137171-61137190 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 61137011 | 61137750 | 61137011 | ID=MS.gene20895 |
| chr1.3 | mRNA | 61137011 | 61137750 | 61137011 | ID=MS.gene20895.t1;Parent=MS.gene20895 |
| chr1.3 | exon | 61137011 | 61137050 | 61137011 | ID=MS.gene20895.t1.exon1;Parent=MS.gene20895.t1 |
| chr1.3 | CDS | 61137011 | 61137050 | 61137011 | ID=cds.MS.gene20895.t1;Parent=MS.gene20895.t1 |
| chr1.3 | exon | 61137135 | 61137190 | 61137135 | ID=MS.gene20895.t1.exon2;Parent=MS.gene20895.t1 |
| chr1.3 | CDS | 61137135 | 61137190 | 61137135 | ID=cds.MS.gene20895.t1;Parent=MS.gene20895.t1 |
| chr1.3 | exon | 61137279 | 61137331 | 61137279 | ID=MS.gene20895.t1.exon3;Parent=MS.gene20895.t1 |
| chr1.3 | CDS | 61137279 | 61137331 | 61137279 | ID=cds.MS.gene20895.t1;Parent=MS.gene20895.t1 |
| chr1.3 | exon | 61137439 | 61137557 | 61137439 | ID=MS.gene20895.t1.exon4;Parent=MS.gene20895.t1 |
| chr1.3 | CDS | 61137439 | 61137557 | 61137439 | ID=cds.MS.gene20895.t1;Parent=MS.gene20895.t1 |
| chr1.3 | exon | 61137653 | 61137750 | 61137653 | ID=MS.gene20895.t1.exon5;Parent=MS.gene20895.t1 |
| chr1.3 | CDS | 61137653 | 61137750 | 61137653 | ID=cds.MS.gene20895.t1;Parent=MS.gene20895.t1 |
| Gene Sequence |
| Protein sequence |
