AlfalfaGEDB | MS.gene051982

M. sativa cultivar XinJiangDaYe / MS.gene051982

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051982.t1	XP_003597545.2	94.5	165	9	0	1	165	1	165	6.30E-81	310.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051982.t1	G7IS16	94.5	165	9	0	1	165	1	165	4.5e-81	310.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051982	MS.gene063123	-0.816	6.93E-52	-1.69E-46
MS.gene051982	MS.gene20895	0.812146	4.91E-51	-1.69E-46
MS.gene051982	MS.gene21434	0.812702	3.71E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051982.t1	MTR_2g099440	94.545	165	9	0	1	165	1	165	1.63e-114	322

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051982.t1	AT2G43540	48.235	170	80	3	1	164	1	168	5.44e-43	140

Find 52 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGTTCCTGTTGGTGTTGC+TGG	0.251042	2.2:-3512058	MS.gene051982:CDS
CTTCGAAGGTGTGTTGTGTT+AGG	0.256702	2.2:+3512326	None:intergenic
TCTTTACACTGACGCTGTTT+TGG	0.296002	2.2:-3512107	MS.gene051982:CDS
CGACAGCGTCCTTCGACTTC+CGG	0.324677	2.2:-3512152	MS.gene051982:CDS
GTCGCAGGCTGAGTTGAATT+CGG	0.348824	2.2:+3512372	None:intergenic
TCGCAGGCTGAGTTGAATTC+GGG	0.354900	2.2:+3512373	None:intergenic
GACGCTGTCGCGGTTGTGAT+CGG	0.362382	2.2:+3512165	None:intergenic
GATCTGGTCCTGTGCTGCTT+GGG	0.387437	2.2:-3512009	MS.gene051982:CDS
ATTGGTGCTGTTGCAAGATC+TGG	0.425634	2.2:-3512025	MS.gene051982:CDS
AGATCTGGTCCTGTGCTGCT+TGG	0.427958	2.2:-3512010	MS.gene051982:CDS
TTCGAAGGTGTGTTGTGTTA+GGG	0.436255	2.2:+3512327	None:intergenic
ACGCTGTCGCGGTTGTGATC+GGG	0.437599	2.2:+3512166	None:intergenic
GGCGGAGTGCGGAGTCAACT+TGG	0.443036	2.2:+3512200	None:intergenic
GTTGCTGGGATCGTTGGAAT+TGG	0.443473	2.2:-3512043	MS.gene051982:CDS
GGTGGTTGGTGAGGAGAGTG+TGG	0.443994	2.2:+3512263	None:intergenic
TGGGTTCGATCGGGAGGAGA+TGG	0.444187	2.2:+3512220	None:intergenic
ATGGGACCCAGTTGTGGATG+AGG	0.463184	2.2:+3512239	None:intergenic
ACGATCCCAGCAACACCAAC+AGG	0.463266	2.2:+3512052	None:intergenic
TAGGGAGAGGCAGTGGATGA+AGG	0.474118	2.2:+3512345	None:intergenic
GAGTTCCTGTTGGTGTTGCT+GGG	0.475369	2.2:-3512057	MS.gene051982:CDS
CGAGCCCATTCCTTGAAAAC+CGG	0.493356	2.2:+3512133	None:intergenic
GTGGATGAAGGTTGTGTCGC+AGG	0.509502	2.2:+3512357	None:intergenic
GGAGTCAACTTGGGTTCGAT+CGG	0.509646	2.2:+3512210	None:intergenic
GAGTCAACTTGGGTTCGATC+GGG	0.510610	2.2:+3512211	None:intergenic
GTTGGTGTTGCTGGGATCGT+TGG	0.512435	2.2:-3512049	MS.gene051982:CDS
GGGTTCGATCGGGAGGAGAT+GGG	0.520000	2.2:+3512221	None:intergenic
TTTGTTGGAGCTTACTCCAT+CGG	0.520191	2.2:-3511983	MS.gene051982:CDS
GTTGTGTTAGGGAGAGGCAG+TGG	0.528825	2.2:+3512338	None:intergenic
ACAAAACTCCCAAGCAGCAC+AGG	0.530149	2.2:+3512001	None:intergenic
CGCGGTTGTGATCGGGTGGA+AGG	0.531290	2.2:+3512173	None:intergenic
TGGGACCCAGTTGTGGATGA+GGG	0.534470	2.2:+3512240	None:intergenic
TGTGATCGGGTGGAAGGACA+CGG	0.537395	2.2:+3512179	None:intergenic
GGACCCAGTTGTGGATGAGG+GGG	0.538461	2.2:+3512242	None:intergenic
AGTGTGGATTTGATCGGAAG+CGG	0.540019	2.2:+3512279	None:intergenic
GAGGAGATGGGACCCAGTTG+TGG	0.541506	2.2:+3512233	None:intergenic
ATTTATCAATTTAATTTATG+CGG	0.548565	2.2:+3511931	None:intergenic
AGGTGTGTTGTGTTAGGGAG+AGG	0.553651	2.2:+3512332	None:intergenic
TGGAAGGACACGGCGGAGTG+CGG	0.555448	2.2:+3512189	None:intergenic
CGCAGGCTGAGTTGAATTCG+GGG	0.568422	2.2:+3512374	None:intergenic
GCGGAGTGCGGAGTCAACTT+GGG	0.578376	2.2:+3512201	None:intergenic
CCTTGAAAACCGGAAGTCGA+AGG	0.578517	2.2:+3512143	None:intergenic
GGGACCCAGTTGTGGATGAG+GGG	0.591665	2.2:+3512241	None:intergenic
CTAACACAACACACCTTCGA+AGG	0.594264	2.2:-3512325	MS.gene051982:CDS
CTGTCGCGGTTGTGATCGGG+TGG	0.596032	2.2:+3512169	None:intergenic
TCAACTTGGGTTCGATCGGG+AGG	0.602242	2.2:+3512214	None:intergenic
GAAGTCGAAGGACGCTGTCG+CGG	0.617152	2.2:+3512155	None:intergenic
GAGGAGAGTGTGGATTTGAT+CGG	0.617700	2.2:+3512273	None:intergenic
AATAGAAAGAGCAACACCGA+TGG	0.625602	2.2:+3511967	None:intergenic
GTAGGGTAAGACTCCTTCGA+AGG	0.634603	2.2:+3512312	None:intergenic
TTGATTGTAAGAGTTCCTGT+TGG	0.666947	2.2:-3512067	MS.gene051982:CDS
GCAGGCTGAGTTGAATTCGG+GGG	0.679036	2.2:+3512375	None:intergenic
GATCGGGTGGAAGGACACGG+CGG	0.789521	2.2:+3512182	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTGCTCTTTCTATTTTTCT+TGG	-	chr2.2:3512408-3512427	MS.gene051982:CDS	30.0%
	TTGATTGTAAGAGTTCCTGT+TGG	-	chr2.2:3512300-3512319	MS.gene051982:CDS	35.0%
	AATAGAAAGAGCAACACCGA+TGG	+	chr2.2:3512403-3512422	None:intergenic	40.0%
	TTTGTTGGAGCTTACTCCAT+CGG	-	chr2.2:3512384-3512403	MS.gene051982:CDS	40.0%
!	TCTTTACACTGACGCTGTTT+TGG	-	chr2.2:3512260-3512279	MS.gene051982:CDS	40.0%
!	TTCGAAGGTGTGTTGTGTTA+GGG	+	chr2.2:3512043-3512062	None:intergenic	40.0%
!!	ACTTCCGGTTTTCAAGGAAT+GGG	-	chr2.2:3512230-3512249	MS.gene051982:CDS	40.0%
	AGTGTGGATTTGATCGGAAG+CGG	+	chr2.2:3512091-3512110	None:intergenic	45.0%
	CTAACACAACACACCTTCGA+AGG	-	chr2.2:3512042-3512061	MS.gene051982:CDS	45.0%
!	CTTCGAAGGTGTGTTGTGTT+AGG	+	chr2.2:3512044-3512063	None:intergenic	45.0%
!	GAGGAGAGTGTGGATTTGAT+CGG	+	chr2.2:3512097-3512116	None:intergenic	45.0%
!!	ATTGGTGCTGTTGCAAGATC+TGG	-	chr2.2:3512342-3512361	MS.gene051982:CDS	45.0%
!!	GACTTCCGGTTTTCAAGGAA+TGG	-	chr2.2:3512229-3512248	MS.gene051982:CDS	45.0%
!!!	GAAGCGGTTTTGAGTTGGTA+GGG	+	chr2.2:3512075-3512094	None:intergenic	45.0%
	ACAAAACTCCCAAGCAGCAC+AGG	+	chr2.2:3512369-3512388	None:intergenic	50.0%
	AGGTGTGTTGTGTTAGGGAG+AGG	+	chr2.2:3512038-3512057	None:intergenic	50.0%
	AGTTGAATTCGGGGGAGGTT+TGG	+	chr2.2:3511987-3512006	None:intergenic	50.0%
	CCTTGAAAACCGGAAGTCGA+AGG	+	chr2.2:3512227-3512246	None:intergenic	50.0%
	CGAGCCCATTCCTTGAAAAC+CGG	+	chr2.2:3512237-3512256	None:intergenic	50.0%
	GAGTCAACTTGGGTTCGATC+GGG	+	chr2.2:3512159-3512178	None:intergenic	50.0%
	GGAGTCAACTTGGGTTCGAT+CGG	+	chr2.2:3512160-3512179	None:intergenic	50.0%
	GTAGGGTAAGACTCCTTCGA+AGG	+	chr2.2:3512058-3512077	None:intergenic	50.0%
	GTTGCTGGGATCGTTGGAAT+TGG	-	chr2.2:3512324-3512343	MS.gene051982:CDS	50.0%
!	CCTTCGACTTCCGGTTTTCA+AGG	-	chr2.2:3512224-3512243	MS.gene051982:CDS	50.0%
!	GTCGCAGGCTGAGTTGAATT+CGG	+	chr2.2:3511998-3512017	None:intergenic	50.0%
!	TCGCAGGCTGAGTTGAATTC+GGG	+	chr2.2:3511997-3512016	None:intergenic	50.0%
!!	AGAGTTCCTGTTGGTGTTGC+TGG	-	chr2.2:3512309-3512328	MS.gene051982:CDS	50.0%
!!	CGGTTTTCAAGGAATGGGCT+CGG	-	chr2.2:3512235-3512254	MS.gene051982:CDS	50.0%
!!	GAGTTCCTGTTGGTGTTGCT+GGG	-	chr2.2:3512310-3512329	MS.gene051982:CDS	50.0%
!!!	GATCGGAAGCGGTTTTGAGT+TGG	+	chr2.2:3512080-3512099	None:intergenic	50.0%
!!!	GGAAGCGGTTTTGAGTTGGT+AGG	+	chr2.2:3512076-3512095	None:intergenic	50.0%
!!!	GTGCTGCTTGGGAGTTTTGT+TGG	-	chr2.2:3512369-3512388	MS.gene051982:CDS	50.0%
	ACGATCCCAGCAACACCAAC+AGG	+	chr2.2:3512318-3512337	None:intergenic	55.0%
	ATGGGACCCAGTTGTGGATG+AGG	+	chr2.2:3512131-3512150	None:intergenic	55.0%
	GTGGATGAAGGTTGTGTCGC+AGG	+	chr2.2:3512013-3512032	None:intergenic	55.0%
	GTTGTGTTAGGGAGAGGCAG+TGG	+	chr2.2:3512032-3512051	None:intergenic	55.0%
	TAGGGAGAGGCAGTGGATGA+AGG	+	chr2.2:3512025-3512044	None:intergenic	55.0%
	TCAACTTGGGTTCGATCGGG+AGG	+	chr2.2:3512156-3512175	None:intergenic	55.0%
	TGGGACCCAGTTGTGGATGA+GGG	+	chr2.2:3512130-3512149	None:intergenic	55.0%
	TGTGATCGGGTGGAAGGACA+CGG	+	chr2.2:3512191-3512210	None:intergenic	55.0%
!	AGATCTGGTCCTGTGCTGCT+TGG	-	chr2.2:3512357-3512376	MS.gene051982:CDS	55.0%
!	CGCAGGCTGAGTTGAATTCG+GGG	+	chr2.2:3511996-3512015	None:intergenic	55.0%
!	GCAGGCTGAGTTGAATTCGG+GGG	+	chr2.2:3511995-3512014	None:intergenic	55.0%
!!	GATCTGGTCCTGTGCTGCTT+GGG	-	chr2.2:3512358-3512377	MS.gene051982:CDS	55.0%
!!	GTTGGTGTTGCTGGGATCGT+TGG	-	chr2.2:3512318-3512337	MS.gene051982:CDS	55.0%
!!!	GACGCTGTTTTGGCTTCTGC+TGG	-	chr2.2:3512270-3512289	MS.gene051982:CDS	55.0%
	ACCACCCCCTCATCCACAAC+TGG	-	chr2.2:3512121-3512140	MS.gene051982:CDS	60.0%
	ACGCTGTCGCGGTTGTGATC+GGG	+	chr2.2:3512204-3512223	None:intergenic	60.0%
	CCACCCCCTCATCCACAACT+GGG	-	chr2.2:3512122-3512141	MS.gene051982:CDS	60.0%
	CGACAGCGTCCTTCGACTTC+CGG	-	chr2.2:3512215-3512234	MS.gene051982:CDS	60.0%
	GAAGTCGAAGGACGCTGTCG+CGG	+	chr2.2:3512215-3512234	None:intergenic	60.0%
	GACGCTGTCGCGGTTGTGAT+CGG	+	chr2.2:3512205-3512224	None:intergenic	60.0%
	GAGGAGATGGGACCCAGTTG+TGG	+	chr2.2:3512137-3512156	None:intergenic	60.0%
	GCGGAGTGCGGAGTCAACTT+GGG	+	chr2.2:3512169-3512188	None:intergenic	60.0%
	GGACCCAGTTGTGGATGAGG+GGG	+	chr2.2:3512128-3512147	None:intergenic	60.0%
	GGGACCCAGTTGTGGATGAG+GGG	+	chr2.2:3512129-3512148	None:intergenic	60.0%
	GGGTTCGATCGGGAGGAGAT+GGG	+	chr2.2:3512149-3512168	None:intergenic	60.0%
	GTTGTGGATGAGGGGGTGGT+TGG	+	chr2.2:3512121-3512140	None:intergenic	60.0%
	TGGGTTCGATCGGGAGGAGA+TGG	+	chr2.2:3512150-3512169	None:intergenic	60.0%
!	GGCTGAGTTGAATTCGGGGG+AGG	+	chr2.2:3511992-3512011	None:intergenic	60.0%
!!	GGTGGTTGGTGAGGAGAGTG+TGG	+	chr2.2:3512107-3512126	None:intergenic	60.0%
	CCCAGTTGTGGATGAGGGGG+TGG	+	chr2.2:3512125-3512144	None:intergenic	65.0%
	CGCGGTTGTGATCGGGTGGA+AGG	+	chr2.2:3512197-3512216	None:intergenic	65.0%
	CTGTCGCGGTTGTGATCGGG+TGG	+	chr2.2:3512201-3512220	None:intergenic	65.0%
	GATCGGGTGGAAGGACACGG+CGG	+	chr2.2:3512188-3512207	None:intergenic	65.0%
	GGCGGAGTGCGGAGTCAACT+TGG	+	chr2.2:3512170-3512189	None:intergenic	65.0%
	TGGAAGGACACGGCGGAGTG+CGG	+	chr2.2:3512181-3512200	None:intergenic	65.0%
!	GGATGAGGGGGTGGTTGGTG+AGG	+	chr2.2:3512116-3512135	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	3511946	3512443	3511946	ID=MS.gene051982
chr2.2	mRNA	3511946	3512443	3511946	ID=MS.gene051982.t1;Parent=MS.gene051982
chr2.2	exon	3511946	3512443	3511946	ID=MS.gene051982.t1.exon1;Parent=MS.gene051982.t1
chr2.2	CDS	3511946	3512443	3511946	ID=cds.MS.gene051982.t1;Parent=MS.gene051982.t1

Gene Sequence

>MS.gene051982

ATGGGGTGTTCAGCATCAAAGAGAGTAAACAACACTTTCCAAACCTCCCCCGAATTCAACTCAGCCTGCGACACAACCTTCATCCACTGCCTCTCCCTAACACAACACACCTTCGAAGGAGTCTTACCCTACCAACTCAAAACCGCTTCCGATCAAATCCACACTCTCCTCACCAACCACCCCCTCATCCACAACTGGGTCCCATCTCCTCCCGATCGAACCCAAGTTGACTCCGCACTCCGCCGTGTCCTTCCACCCGATCACAACCGCGACAGCGTCCTTCGACTTCCGGTTTTCAAGGAATGGGCTCGGTGTCTTTACACTGACGCTGTTTTGGCTTCTGCTGGTAAAGCGTTGATTGTAAGAGTTCCTGTTGGTGTTGCTGGGATCGTTGGAATTGGTGCTGTTGCAAGATCTGGTCCTGTGCTGCTTGGGAGTTTTGTTGGAGCTTACTCCATCGGTGTTGCTCTTTCTATTTTTCTTGGTTTATCCGCATAA

Protein sequence

>MS.gene051982.t1

MGCSASKRVNNTFQTSPEFNSACDTTFIHCLSLTQHTFEGVLPYQLKTASDQIHTLLTNHPLIHNWVPSPPDRTQVDSALRRVLPPDHNRDSVLRLPVFKEWARCLYTDAVLASAGKALIVRVPVGVAGIVGIGAVARSGPVLLGSFVGAYSIGVALSIFLGLSA