Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20941.t1 | XP_013469106.1 | 98.5 | 137 | 2 | 0 | 1 | 137 | 1 | 137 | 1.40E-62 | 248.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20941.t1 | A0A072VMA2 | 98.5 | 137 | 2 | 0 | 1 | 137 | 1 | 137 | 1.0e-62 | 248.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050838 | MS.gene20941 | 0.820393 | 7.02E-53 | -1.69E-46 |
| MS.gene053786 | MS.gene20941 | 0.808923 | 2.44E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20941.t1 | MTR_1g085355 | 98.540 | 137 | 2 | 0 | 1 | 137 | 1 | 137 | 3.13e-94 | 268 |
| MS.gene20941.t1 | MTR_4g118050 | 97.810 | 137 | 2 | 1 | 1 | 137 | 1 | 136 | 1.70e-91 | 261 |
| MS.gene20941.t1 | MTR_1g085440 | 94.161 | 137 | 7 | 1 | 1 | 137 | 1 | 136 | 7.95e-89 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20941.t1 | AT5G03050 | 58.730 | 126 | 47 | 2 | 10 | 132 | 11 | 134 | 1.44e-42 | 137 |
| MS.gene20941.t1 | AT5G03050 | 58.730 | 126 | 47 | 2 | 10 | 132 | 11 | 134 | 1.44e-42 | 137 |
Find 22 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAGGGAAAACAATGTTTA+AGG | 0.261813 | 1.3:+60681285 | MS.gene20941:CDS |
| GTATCTGAGCTCTTGGAATC+AGG | 0.345471 | 1.3:+60681268 | MS.gene20941:CDS |
| CCAAGTTGGGTCTGAAGAAA+TGG | 0.369614 | 1.3:+60681095 | MS.gene20941:CDS |
| AGCATTAGCTTCCTCATTTG+AGG | 0.397998 | 1.3:-60681515 | None:intergenic |
| TAACCAATGTCAGCTCAAAA+AGG | 0.399910 | 1.3:+60680854 | None:intergenic |
| CTTGTAGGTATCTGAGCTCT+TGG | 0.437315 | 1.3:+60681261 | MS.gene20941:intron |
| CCATTTCTTCAGACCCAACT+TGG | 0.441813 | 1.3:-60681095 | None:intergenic |
| AAGCAGGATTCACAAGGAAC+AGG | 0.472958 | 1.3:+60681447 | MS.gene20941:intron |
| GGTTTGAGACTAACCAAGTT+GGG | 0.494830 | 1.3:+60681082 | MS.gene20941:CDS |
| TATCTGAGCTCTTGGAATCA+GGG | 0.503680 | 1.3:+60681269 | MS.gene20941:CDS |
| GCATGGACAGTGGAGCTTCA+AGG | 0.522029 | 1.3:+60680879 | MS.gene20941:CDS |
| TCAAGGAAACGTATGAAACC+TGG | 0.524964 | 1.3:+60680896 | MS.gene20941:CDS |
| ATCGCAGATTGTCCTTGTAC+AGG | 0.525946 | 1.3:+60681053 | MS.gene20941:CDS |
| CAAACCCTTCCTCCTGTACA+AGG | 0.529759 | 1.3:-60681065 | None:intergenic |
| ATTGTCCTTGTACAGGAGGA+AGG | 0.538219 | 1.3:+60681060 | MS.gene20941:CDS |
| TTGTCCTTGTACAGGAGGAA+GGG | 0.558464 | 1.3:+60681061 | MS.gene20941:CDS |
| CAAGGAAACGTATGAAACCT+GGG | 0.567520 | 1.3:+60680897 | MS.gene20941:CDS |
| TCAAATAAGCAGGATTCACA+AGG | 0.592512 | 1.3:+60681441 | MS.gene20941:intron |
| GGGTTTGAGACTAACCAAGT+TGG | 0.597415 | 1.3:+60681081 | MS.gene20941:CDS |
| TGCACTTGATGCCTCAAATG+AGG | 0.607261 | 1.3:+60681504 | MS.gene20941:CDS |
| GCAGATTGTCCTTGTACAGG+AGG | 0.643938 | 1.3:+60681056 | MS.gene20941:CDS |
| AAGGAAACGTATGAAACCTG+GGG | 0.689052 | 1.3:+60680898 | MS.gene20941:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAACATTGAATGTTCAA+TGG | - | chr1.3:60681361-60681380 | None:intergenic | 20.0% |
| !!! | ATGTTTGAAATGTTTAATTG+TGG | + | chr1.3:60681234-60681253 | MS.gene20941:intron | 20.0% |
| !!! | TGTTAGTTTTTGACTTTTTA+TGG | + | chr1.3:60681401-60681420 | MS.gene20941:intron | 20.0% |
| ! | TTTAATTGTGGATTTCTTGT+AGG | + | chr1.3:60681246-60681265 | MS.gene20941:intron | 25.0% |
| !! | CTTTTCTTTTCACAATAAAC+AGG | + | chr1.3:60680999-60681018 | MS.gene20941:intron | 25.0% |
| !!! | CTTTTAACTTTCAAATAAGC+AGG | + | chr1.3:60681431-60681450 | MS.gene20941:intron | 25.0% |
| ATCAGGGAAAACAATGTTTA+AGG | + | chr1.3:60681285-60681304 | MS.gene20941:CDS | 30.0% | |
| ATGACGAGAAAAATGTTGAA+AGG | - | chr1.3:60680939-60680958 | None:intergenic | 30.0% | |
| ! | AGATTGAGAATTTTACTCAG+AGG | + | chr1.3:60681145-60681164 | MS.gene20941:CDS | 30.0% |
| ! | GATTGAGAATTTTACTCAGA+GGG | + | chr1.3:60681146-60681165 | MS.gene20941:CDS | 30.0% |
| TCAAATAAGCAGGATTCACA+AGG | + | chr1.3:60681441-60681460 | MS.gene20941:intron | 35.0% | |
| ! | ACAAGGAACAGGTTGAAAAA+TGG | + | chr1.3:60681458-60681477 | MS.gene20941:CDS | 35.0% |
| AAGGAAACGTATGAAACCTG+GGG | + | chr1.3:60680898-60680917 | MS.gene20941:CDS | 40.0% | |
| CAAGGAAACGTATGAAACCT+GGG | + | chr1.3:60680897-60680916 | MS.gene20941:CDS | 40.0% | |
| GCGAAAATAACAATTACCCC+AGG | - | chr1.3:60680917-60680936 | None:intergenic | 40.0% | |
| TATCTGAGCTCTTGGAATCA+GGG | + | chr1.3:60681269-60681288 | MS.gene20941:CDS | 40.0% | |
| TCAAGGAAACGTATGAAACC+TGG | + | chr1.3:60680896-60680915 | MS.gene20941:CDS | 40.0% | |
| ! | AGCATTAGCTTCCTCATTTG+AGG | - | chr1.3:60681518-60681537 | None:intergenic | 40.0% |
| ! | GGTTTGAGACTAACCAAGTT+GGG | + | chr1.3:60681082-60681101 | MS.gene20941:CDS | 40.0% |
| AAGCAGGATTCACAAGGAAC+AGG | + | chr1.3:60681447-60681466 | MS.gene20941:intron | 45.0% | |
| ATCGCAGATTGTCCTTGTAC+AGG | + | chr1.3:60681053-60681072 | MS.gene20941:CDS | 45.0% | |
| ATTGTCCTTGTACAGGAGGA+AGG | + | chr1.3:60681060-60681079 | MS.gene20941:CDS | 45.0% | |
| CCATTTCTTCAGACCCAACT+TGG | - | chr1.3:60681098-60681117 | None:intergenic | 45.0% | |
| CTTGTAGGTATCTGAGCTCT+TGG | + | chr1.3:60681261-60681280 | MS.gene20941:intron | 45.0% | |
| GTATCTGAGCTCTTGGAATC+AGG | + | chr1.3:60681268-60681287 | MS.gene20941:CDS | 45.0% | |
| GTCAGCTCAAAAAGGAAGCA+TGG | + | chr1.3:60680862-60680881 | MS.gene20941:CDS | 45.0% | |
| TGCACTTGATGCCTCAAATG+AGG | + | chr1.3:60681504-60681523 | MS.gene20941:CDS | 45.0% | |
| TTGTCCTTGTACAGGAGGAA+GGG | + | chr1.3:60681061-60681080 | MS.gene20941:CDS | 45.0% | |
| ! | CAAAAAGGAAGCATGGACAG+TGG | + | chr1.3:60680869-60680888 | MS.gene20941:CDS | 45.0% |
| ! | CCAAGTTGGGTCTGAAGAAA+TGG | + | chr1.3:60681095-60681114 | MS.gene20941:CDS | 45.0% |
| ! | GGAACAGGTTGAAAAATGGC+AGG | + | chr1.3:60681462-60681481 | MS.gene20941:CDS | 45.0% |
| ! | GGGTTTGAGACTAACCAAGT+TGG | + | chr1.3:60681081-60681100 | MS.gene20941:CDS | 45.0% |
| CAAACCCTTCCTCCTGTACA+AGG | - | chr1.3:60681068-60681087 | None:intergenic | 50.0% | |
| GCAGATTGTCCTTGTACAGG+AGG | + | chr1.3:60681056-60681075 | MS.gene20941:CDS | 50.0% | |
| GCATGGACAGTGGAGCTTCA+AGG | + | chr1.3:60680879-60680898 | MS.gene20941:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 60680860 | 60681585 | 60680860 | ID=MS.gene20941 |
| chr1.3 | mRNA | 60680860 | 60681585 | 60680860 | ID=MS.gene20941.t1;Parent=MS.gene20941 |
| chr1.3 | exon | 60680860 | 60680919 | 60680860 | ID=MS.gene20941.t1.exon1;Parent=MS.gene20941.t1 |
| chr1.3 | CDS | 60680860 | 60680919 | 60680860 | ID=cds.MS.gene20941.t1;Parent=MS.gene20941.t1 |
| chr1.3 | exon | 60681021 | 60681167 | 60681021 | ID=MS.gene20941.t1.exon2;Parent=MS.gene20941.t1 |
| chr1.3 | CDS | 60681021 | 60681167 | 60681021 | ID=cds.MS.gene20941.t1;Parent=MS.gene20941.t1 |
| chr1.3 | exon | 60681268 | 60681341 | 60681268 | ID=MS.gene20941.t1.exon3;Parent=MS.gene20941.t1 |
| chr1.3 | CDS | 60681268 | 60681341 | 60681268 | ID=cds.MS.gene20941.t1;Parent=MS.gene20941.t1 |
| chr1.3 | exon | 60681453 | 60681585 | 60681453 | ID=MS.gene20941.t1.exon4;Parent=MS.gene20941.t1 |
| chr1.3 | CDS | 60681453 | 60681585 | 60681453 | ID=cds.MS.gene20941.t1;Parent=MS.gene20941.t1 |
| Gene Sequence |
| Protein sequence |
