Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21209.t1 | RHN53781.1 | 84.1 | 107 | 15 | 1 | 7 | 111 | 34 | 140 | 9.60E-41 | 176 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21209.t1 | A0A396HMR3 | 84.1 | 107 | 15 | 1 | 7 | 111 | 34 | 140 | 6.9e-41 | 176.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050156 | MS.gene21209 | 0.841893 | 3.60E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21209.t1 | MTR_5g013080 | 83.178 | 107 | 16 | 1 | 7 | 111 | 34 | 140 | 4.09e-57 | 175 |
| MS.gene21209.t1 | MTR_5g013140 | 69.524 | 105 | 32 | 0 | 7 | 111 | 147 | 251 | 2.36e-40 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAATGAGACCTACAAAAC+TGG | 0.420267 | 5.1:-6148670 | MS.gene21209:CDS |
| GCAATCATGGAAGTCCTGTT+TGG | 0.422527 | 5.1:-6148734 | MS.gene21209:CDS |
| TATTGAGAATACTTTAAATC+CGG | 0.432788 | 5.1:-6148838 | MS.gene21209:CDS |
| TTGTGTTGGACACAAACTTT+CGG | 0.442750 | 5.1:+6149317 | None:intergenic |
| GTTCACTGTCTTCTTATACT+TGG | 0.447231 | 5.1:+6148758 | None:intergenic |
| AGAAGCATCCTTAACATCAT+TGG | 0.450182 | 5.1:-6148706 | MS.gene21209:CDS |
| GCAGCCATACATGCAACATA+AGG | 0.463012 | 5.1:-6148805 | MS.gene21209:CDS |
| AAGACAGTGAACTGCAATCA+TGG | 0.467236 | 5.1:-6148747 | MS.gene21209:CDS |
| TGGCAGGGCGATGGTAGTGC+TGG | 0.481700 | 5.1:-6148879 | MS.gene21209:intron |
| AGTGCCTTATGTTGCATGTA+TGG | 0.483200 | 5.1:+6148801 | None:intergenic |
| ATCAGTGTCCAATGATGTTA+AGG | 0.486766 | 5.1:+6148698 | None:intergenic |
| GATGCTTCTCCATTCCAAAC+AGG | 0.489089 | 5.1:+6148720 | None:intergenic |
| ACTGTCTTCTTATACTTGGT+CGG | 0.489810 | 5.1:+6148762 | None:intergenic |
| TCGGTTGCACATTCTCGAAC+CGG | 0.514678 | 5.1:+6149336 | None:intergenic |
| CATGGAAGTCCTGTTTGGAA+TGG | 0.547429 | 5.1:-6148729 | MS.gene21209:CDS |
| GTGGAGGTAAATAAAGATGA+AGG | 0.554468 | 5.1:-6149359 | MS.gene21209:CDS |
| TATCAAAAGAGCTATTGTGT+TGG | 0.555066 | 5.1:+6149303 | None:intergenic |
| GGTGGACAATGGCAGGGCGA+TGG | 0.604468 | 5.1:-6148888 | MS.gene21209:intron |
| TTGCATAATGAGGGAAACAG+TGG | 0.626434 | 5.1:-6149378 | None:intergenic |
| CATAATGAGGGAAACAGTGG+AGG | 0.636407 | 5.1:-6149375 | None:intergenic |
| AGGTAAATAAAGATGAAGGC+CGG | 0.649787 | 5.1:-6149355 | MS.gene21209:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGATTAGTAATTTGAAT+TGG | - | chr5.1:6148813-6148832 | MS.gene21209:CDS | 15.0% |
| !! | TATTGAGAATACTTTAAATC+CGG | - | chr5.1:6149195-6149214 | MS.gene21209:intron | 20.0% |
| !!! | TAACTGAATGATCTATTTTT+AGG | + | chr5.1:6149004-6149023 | None:intergenic | 20.0% |
| ! | AAGATGCATAATCAAAATTC+AGG | + | chr5.1:6148854-6148873 | None:intergenic | 25.0% |
| ! | GATTATGCATCTTAGTAATA+TGG | - | chr5.1:6148861-6148880 | MS.gene21209:CDS | 25.0% |
| ! | TGAATGGAAATTGTTTAGTT+TGG | - | chr5.1:6148941-6148960 | MS.gene21209:intron | 25.0% |
| !! | TAGCTCTTTTGATAAAAACA+TGG | - | chr5.1:6148739-6148758 | MS.gene21209:CDS | 25.0% |
| GAGATATTACAAGAGAAATC+AGG | + | chr5.1:6148781-6148800 | None:intergenic | 30.0% | |
| TATCAAAAGAGCTATTGTGT+TGG | + | chr5.1:6148733-6148752 | None:intergenic | 30.0% | |
| ! | CTCTTTTGATAAAAACATGG+AGG | - | chr5.1:6148742-6148761 | MS.gene21209:CDS | 30.0% |
| !! | TAGTTAGTTGATGACATGAA+TGG | - | chr5.1:6148925-6148944 | MS.gene21209:intron | 30.0% |
| !! | TTTAGTTTGGCAAACTTCAA+AGG | - | chr5.1:6148954-6148973 | MS.gene21209:intron | 30.0% |
| AACAGTGAGCTCAAATTACT+TGG | + | chr5.1:6149118-6149137 | None:intergenic | 35.0% | |
| AACTTTCTTCTGTTGAACGT+TGG | + | chr5.1:6149059-6149078 | None:intergenic | 35.0% | |
| ACAGTGAGCTCAAATTACTT+GGG | + | chr5.1:6149117-6149136 | None:intergenic | 35.0% | |
| ACTGTCTTCTTATACTTGGT+CGG | + | chr5.1:6149274-6149293 | None:intergenic | 35.0% | |
| AGGTAAATAAAGATGAAGGC+CGG | - | chr5.1:6148678-6148697 | MS.gene21209:CDS | 35.0% | |
| ATCAGTGTCCAATGATGTTA+AGG | + | chr5.1:6149338-6149357 | None:intergenic | 35.0% | |
| CAGTTAAGTGATGACATGAA+TGG | - | chr5.1:6149018-6149037 | MS.gene21209:intron | 35.0% | |
| CTAACTAATGACCTACTTCA+AGG | + | chr5.1:6148912-6148931 | None:intergenic | 35.0% | |
| GTGGAGGTAAATAAAGATGA+AGG | - | chr5.1:6148674-6148693 | MS.gene21209:CDS | 35.0% | |
| GTTCACTGTCTTCTTATACT+TGG | + | chr5.1:6149278-6149297 | None:intergenic | 35.0% | |
| TTGTGTTGGACACAAACTTT+CGG | + | chr5.1:6148719-6148738 | None:intergenic | 35.0% | |
| ! | TATGGCTGCAAAATCTTTTC+CGG | + | chr5.1:6149217-6149236 | None:intergenic | 35.0% |
| !! | AGAAGCATCCTTAACATCAT+TGG | - | chr5.1:6149327-6149346 | MS.gene21209:CDS | 35.0% |
| AAGACAGTGAACTGCAATCA+TGG | - | chr5.1:6149286-6149305 | MS.gene21209:intron | 40.0% | |
| AGTGCCTTATGTTGCATGTA+TGG | + | chr5.1:6149235-6149254 | None:intergenic | 40.0% | |
| TGCAATGAGACCTACAAAAC+TGG | - | chr5.1:6149363-6149382 | MS.gene21209:CDS | 40.0% | |
| !! | TTGAGCTCACTGTTTTTCTG+TGG | - | chr5.1:6149124-6149143 | MS.gene21209:intron | 40.0% |
| !!! | TGTTTTTCTGTGGTGGACAA+TGG | - | chr5.1:6149134-6149153 | MS.gene21209:intron | 40.0% |
| CATGGAAGTCCTGTTTGGAA+TGG | - | chr5.1:6149304-6149323 | MS.gene21209:CDS | 45.0% | |
| GATGCTTCTCCATTCCAAAC+AGG | + | chr5.1:6149316-6149335 | None:intergenic | 45.0% | |
| GCAATCATGGAAGTCCTGTT+TGG | - | chr5.1:6149299-6149318 | MS.gene21209:CDS | 45.0% | |
| GCAGCCATACATGCAACATA+AGG | - | chr5.1:6149228-6149247 | MS.gene21209:intron | 45.0% | |
| GCTCAATTGCACCTTGAAGT+AGG | - | chr5.1:6148898-6148917 | MS.gene21209:intron | 45.0% | |
| !! | AGCTCACTGTTTTTCTGTGG+TGG | - | chr5.1:6149127-6149146 | MS.gene21209:intron | 45.0% |
| TCGGTTGCACATTCTCGAAC+CGG | + | chr5.1:6148700-6148719 | None:intergenic | 50.0% | |
| !! | TTCTGTGGTGGACAATGGCA+GGG | - | chr5.1:6149139-6149158 | MS.gene21209:intron | 50.0% |
| !! | TTTCTGTGGTGGACAATGGC+AGG | - | chr5.1:6149138-6149157 | MS.gene21209:intron | 50.0% |
| GGTGGACAATGGCAGGGCGA+TGG | - | chr5.1:6149145-6149164 | MS.gene21209:intron | 65.0% | |
| ! | TGGCAGGGCGATGGTAGTGC+TGG | - | chr5.1:6149154-6149173 | MS.gene21209:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 6148662 | 6149393 | 6148662 | ID=MS.gene21209 |
| chr5.1 | mRNA | 6148662 | 6149393 | 6148662 | ID=MS.gene21209.t1;Parent=MS.gene21209 |
| chr5.1 | exon | 6149292 | 6149393 | 6149292 | ID=MS.gene21209.t1.exon1;Parent=MS.gene21209.t1 |
| chr5.1 | CDS | 6149292 | 6149393 | 6149292 | ID=cds.MS.gene21209.t1;Parent=MS.gene21209.t1 |
| chr5.1 | exon | 6148662 | 6148895 | 6148662 | ID=MS.gene21209.t1.exon2;Parent=MS.gene21209.t1 |
| chr5.1 | CDS | 6148662 | 6148895 | 6148662 | ID=cds.MS.gene21209.t1;Parent=MS.gene21209.t1 |
| Gene Sequence |
| Protein sequence |
