AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050156


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050156.t1 MTR_5g013080 83.178 107 16 1 37 141 34 140 3.12e-55 172
MS.gene050156.t1 MTR_5g013140 69.524 105 32 0 37 141 147 251 8.76e-39 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGCGGTTGTGTTGGGGCT+GGG 0.255557 5.4:-6938595 MS.gene050156:CDS
TTGCGGTTGTGTTGGGGCTG+GGG 0.279823 5.4:-6938594 MS.gene050156:CDS
TGACATATTCTCCCCTTTGC+TGG 0.282160 5.4:+6938284 None:intergenic
TGCGGTTGTGTTGGGGCTGG+GGG 0.306692 5.4:-6938593 MS.gene050156:CDS
GGTTGCGGTTGTGTTGGGGC+TGG 0.307572 5.4:-6938596 MS.gene050156:CDS
AATGTGGTTGCGGTTGTGTT+GGG 0.400000 5.4:-6938601 MS.gene050156:CDS
TGCAATGAGACCTACAAAAC+TGG 0.420267 5.4:-6937529 MS.gene050156:CDS
GCAATCATGGAAGTCCTGTT+TGG 0.422527 5.4:-6937593 MS.gene050156:CDS
TATTGAGAATACTTTAAATC+CGG 0.432788 5.4:-6937697 MS.gene050156:CDS
CAATGTGGTTGCGGTTGTGT+TGG 0.440708 5.4:-6938602 MS.gene050156:CDS
TTGTGTTGGACACAAACTTT+CGG 0.442750 5.4:+6938176 None:intergenic
GTTCACTGTCTTCTTATACT+TGG 0.447231 5.4:+6937617 None:intergenic
AGAAGCATGCTTAACATCAT+TGG 0.458517 5.4:-6937565 MS.gene050156:CDS
GCAGCCATACATGCAACATA+AGG 0.463012 5.4:-6937664 MS.gene050156:CDS
AAGACAGTGAACTGCAATCA+TGG 0.467236 5.4:-6937606 MS.gene050156:CDS
CATGCTTCTCCATTCCAAAC+AGG 0.474983 5.4:+6937579 None:intergenic
TGAGAAGAATTTGCATAATG+AGG 0.475091 5.4:-6938247 MS.gene050156:CDS
GAGAAGAATTTGCATAATGA+GGG 0.477486 5.4:-6938246 MS.gene050156:CDS
TGGCAGGGCGATGGTAGTGC+TGG 0.481700 5.4:-6937738 MS.gene050156:intron
AGTGCCTTATGTTGCATGTA+TGG 0.483200 5.4:+6937660 None:intergenic
ACTGTCTTCTTATACTTGGT+CGG 0.489810 5.4:+6937621 None:intergenic
GCGGTTGTGTTGGGGCTGGG+GGG 0.493017 5.4:-6938592 MS.gene050156:intron
TCGGTTGCACATTCTCGAAC+CGG 0.514678 5.4:+6938195 None:intergenic
TGGATATCCAGTCCAGCAAA+GGG 0.518657 5.4:-6938296 MS.gene050156:intron
TTTGGGATGCAATGTGGTTG+CGG 0.530367 5.4:-6938611 None:intergenic
CATGGAAGTCCTGTTTGGAA+TGG 0.547429 5.4:-6937588 MS.gene050156:CDS
GTGGAGGTAAATAAAGATGA+AGG 0.554468 5.4:-6938218 MS.gene050156:CDS
TATCAAAAGAGCTATTGTGT+TGG 0.555066 5.4:+6938162 None:intergenic
GTGGATATCCAGTCCAGCAA+AGG 0.573297 5.4:-6938297 MS.gene050156:intron
ATGTGGTTGCGGTTGTGTTG+GGG 0.574322 5.4:-6938600 MS.gene050156:CDS
GGTGGACAATGGCAGGGCGA+TGG 0.604468 5.4:-6937747 MS.gene050156:intron
TTGCATAATGAGGGAAACAG+TGG 0.626434 5.4:-6938237 MS.gene050156:CDS
CATAATGAGGGAAACAGTGG+AGG 0.636407 5.4:-6938234 MS.gene050156:CDS
AGGTAAATAAAGATGAAGGC+CGG 0.649787 5.4:-6938214 MS.gene050156:CDS
GGATATCCAGTCCAGCAAAG+GGG 0.724735 5.4:-6938295 MS.gene050156:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGATTAGTAATTTGAAT+TGG - chr5.4:6938047-6938066 MS.gene050156:intron 15.0%
!! TTAATAGATAGATTATATGA+AGG + chr5.4:6937591-6937610 None:intergenic 15.0%
!! TATTGAGAATACTTTAAATC+CGG - chr5.4:6938429-6938448 MS.gene050156:intron 20.0%
!!! TAACTGAATGATCTATTTTT+AGG + chr5.4:6938238-6938257 None:intergenic 20.0%
!!! TGAAATTCTAAAGTTATTAG+TGG - chr5.4:6937810-6937829 MS.gene050156:intron 20.0%
! AACTTGGAAGAAAAAAAAGT+AGG + chr5.4:6937731-6937750 None:intergenic 25.0%
! AAGATGCATAATCAAAATTC+AGG + chr5.4:6938088-6938107 None:intergenic 25.0%
! GATTATGCATCTTAGTAATA+TGG - chr5.4:6938095-6938114 MS.gene050156:intron 25.0%
! TCTATTTCACTAAGACTTTA+TGG + chr5.4:6937789-6937808 None:intergenic 25.0%
! TGAATGGAAATTGTTTAGTT+TGG - chr5.4:6938175-6938194 MS.gene050156:CDS 25.0%
! TTACTCTCAATTAACAAACT+TGG + chr5.4:6937747-6937766 None:intergenic 25.0%
!! TAGCTCTTTTGATAAAAACA+TGG - chr5.4:6937973-6937992 MS.gene050156:intron 25.0%
!!! ATTTGTTTGCTGTAGTATAT+AGG - chr5.4:6937692-6937711 MS.gene050156:CDS 25.0%
!!! TTTGTTTGCTGTAGTATATA+GGG - chr5.4:6937693-6937712 MS.gene050156:CDS 25.0%
CTATCTATTAAGATGCAAAG+TGG - chr5.4:6937600-6937619 MS.gene050156:CDS 30.0%
GAGAAGAATTTGCATAATGA+GGG - chr5.4:6937880-6937899 MS.gene050156:intron 30.0%
GAGATATTACAAGAGAAATC+AGG + chr5.4:6938015-6938034 None:intergenic 30.0%
TATCAAAAGAGCTATTGTGT+TGG + chr5.4:6937967-6937986 None:intergenic 30.0%
TGAGAAGAATTTGCATAATG+AGG - chr5.4:6937879-6937898 MS.gene050156:intron 30.0%
! CTCTTTTGATAAAAACATGG+AGG - chr5.4:6937976-6937995 MS.gene050156:intron 30.0%
!! TAGTTAGTTGATGACATGAA+TGG - chr5.4:6938159-6938178 MS.gene050156:CDS 30.0%
!! TTGTTTGCTGTAGTATATAG+GGG - chr5.4:6937694-6937713 MS.gene050156:CDS 30.0%
!! TTTAGTTTGGCAAACTTCAA+AGG - chr5.4:6938188-6938207 MS.gene050156:CDS 30.0%
AACAGTGAGCTCAAATTACT+TGG + chr5.4:6938352-6938371 None:intergenic 35.0%
AACTTTCTTCTGTTGAACGT+TGG + chr5.4:6938293-6938312 None:intergenic 35.0%
ACAGTGAGCTCAAATTACTT+GGG + chr5.4:6938351-6938370 None:intergenic 35.0%
ACTGTCTTCTTATACTTGGT+CGG + chr5.4:6938508-6938527 None:intergenic 35.0%
AGGTAAATAAAGATGAAGGC+CGG - chr5.4:6937912-6937931 MS.gene050156:intron 35.0%
AGTTTGAAAGGGAGATAAGA+GGG + chr5.4:6937651-6937670 None:intergenic 35.0%
ATCTCCCTTTCAAACTAGAA+AGG - chr5.4:6937655-6937674 MS.gene050156:CDS 35.0%
CAGTTAAGTGATGACATGAA+TGG - chr5.4:6938252-6938271 MS.gene050156:CDS 35.0%
CTAACTAATGACCTACTTCA+AGG + chr5.4:6938146-6938165 None:intergenic 35.0%
GTGGAGGTAAATAAAGATGA+AGG - chr5.4:6937908-6937927 MS.gene050156:intron 35.0%
GTTCACTGTCTTCTTATACT+TGG + chr5.4:6938512-6938531 None:intergenic 35.0%
TAGTTTGAAAGGGAGATAAG+AGG + chr5.4:6937652-6937671 None:intergenic 35.0%
TTGTGTTGGACACAAACTTT+CGG + chr5.4:6937953-6937972 None:intergenic 35.0%
! AGAAGCATGCTTAACATCAT+TGG - chr5.4:6938561-6938580 MS.gene050156:intron 35.0%
! TATGGCTGCAAAATCTTTTC+CGG + chr5.4:6938451-6938470 None:intergenic 35.0%
!! TGCACCTTTCTAGTTTGAAA+GGG + chr5.4:6937662-6937681 None:intergenic 35.0%
!! TTGCACCTTTCTAGTTTGAA+AGG + chr5.4:6937663-6937682 None:intergenic 35.0%
AAGACAGTGAACTGCAATCA+TGG - chr5.4:6938520-6938539 MS.gene050156:intron 40.0%
AGTGCCTTATGTTGCATGTA+TGG + chr5.4:6938469-6938488 None:intergenic 40.0%
GTGGTATACAGTATTCTCAG+TGG - chr5.4:6937619-6937638 MS.gene050156:CDS 40.0%
TGCAATGAGACCTACAAAAC+TGG - chr5.4:6938597-6938616 MS.gene050156:CDS 40.0%
TTGCATAATGAGGGAAACAG+TGG - chr5.4:6937889-6937908 MS.gene050156:intron 40.0%
TTGCTGTAGTATATAGGGGA+GGG - chr5.4:6937698-6937717 MS.gene050156:CDS 40.0%
! TTTGCTGTAGTATATAGGGG+AGG - chr5.4:6937697-6937716 MS.gene050156:CDS 40.0%
!! TTGAGCTCACTGTTTTTCTG+TGG - chr5.4:6938358-6938377 MS.gene050156:intron 40.0%
!!! TGTTTTTCTGTGGTGGACAA+TGG - chr5.4:6938368-6938387 MS.gene050156:intron 40.0%
AATGTGGTTGCGGTTGTGTT+GGG - chr5.4:6937525-6937544 MS.gene050156:CDS 45.0%
CATAATGAGGGAAACAGTGG+AGG - chr5.4:6937892-6937911 MS.gene050156:intron 45.0%
CATGCTTCTCCATTCCAAAC+AGG + chr5.4:6938550-6938569 None:intergenic 45.0%
CATGGAAGTCCTGTTTGGAA+TGG - chr5.4:6938538-6938557 MS.gene050156:intron 45.0%
GCAATCATGGAAGTCCTGTT+TGG - chr5.4:6938533-6938552 MS.gene050156:intron 45.0%
GCAGCCATACATGCAACATA+AGG - chr5.4:6938462-6938481 MS.gene050156:intron 45.0%
GCTCAATTGCACCTTGAAGT+AGG - chr5.4:6938132-6938151 MS.gene050156:intron 45.0%
TGACATATTCTCCCCTTTGC+TGG + chr5.4:6937845-6937864 None:intergenic 45.0%
TGGATATCCAGTCCAGCAAA+GGG - chr5.4:6937830-6937849 MS.gene050156:intron 45.0%
!! AGCTCACTGTTTTTCTGTGG+TGG - chr5.4:6938361-6938380 MS.gene050156:intron 45.0%
ATGTGGTTGCGGTTGTGTTG+GGG - chr5.4:6937526-6937545 MS.gene050156:CDS 50.0%
CAATGTGGTTGCGGTTGTGT+TGG - chr5.4:6937524-6937543 MS.gene050156:CDS 50.0%
GGATATCCAGTCCAGCAAAG+GGG - chr5.4:6937831-6937850 MS.gene050156:intron 50.0%
GTGGATATCCAGTCCAGCAA+AGG - chr5.4:6937829-6937848 MS.gene050156:intron 50.0%
TATTCTCCCCTTTGCTGGAC+TGG + chr5.4:6937840-6937859 None:intergenic 50.0%
TCGGTTGCACATTCTCGAAC+CGG + chr5.4:6937934-6937953 None:intergenic 50.0%
!! TTCTGTGGTGGACAATGGCA+GGG - chr5.4:6938373-6938392 MS.gene050156:intron 50.0%
!! TTTCTGTGGTGGACAATGGC+AGG - chr5.4:6938372-6938391 MS.gene050156:intron 50.0%
GTTGCGGTTGTGTTGGGGCT+GGG - chr5.4:6937531-6937550 MS.gene050156:CDS 60.0%
TTGCGGTTGTGTTGGGGCTG+GGG - chr5.4:6937532-6937551 MS.gene050156:CDS 60.0%
GGTGGACAATGGCAGGGCGA+TGG - chr5.4:6938379-6938398 MS.gene050156:intron 65.0%
GGTTGCGGTTGTGTTGGGGC+TGG - chr5.4:6937530-6937549 MS.gene050156:CDS 65.0%
TGCGGTTGTGTTGGGGCTGG+GGG - chr5.4:6937533-6937552 MS.gene050156:CDS 65.0%
! CTGGGGGGTACGTGTGAGCT+TGG - chr5.4:6937549-6937568 MS.gene050156:CDS 65.0%
! TGGCAGGGCGATGGTAGTGC+TGG - chr5.4:6938388-6938407 MS.gene050156:intron 65.0%
GCGGTTGTGTTGGGGCTGGG+GGG - chr5.4:6937534-6937553 MS.gene050156:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 6937521 6938627 6937521 ID=MS.gene050156
chr5.4 mRNA 6937521 6938627 6937521 ID=MS.gene050156.t1;Parent=MS.gene050156
chr5.4 exon 6938593 6938627 6938593 ID=MS.gene050156.t1.exon1;Parent=MS.gene050156.t1
chr5.4 CDS 6938593 6938627 6938593 ID=cds.MS.gene050156.t1;Parent=MS.gene050156.t1
chr5.4 exon 6938151 6938307 6938151 ID=MS.gene050156.t1.exon2;Parent=MS.gene050156.t1
chr5.4 CDS 6938151 6938307 6938151 ID=cds.MS.gene050156.t1;Parent=MS.gene050156.t1
chr5.4 exon 6937521 6937754 6937521 ID=MS.gene050156.t1.exon3;Parent=MS.gene050156.t1
chr5.4 CDS 6937521 6937754 6937521 ID=cds.MS.gene050156.t1;Parent=MS.gene050156.t1
Gene Sequence

>MS.gene050156

ATGCAATGTGGTTGCGGTTGTGTTGGGGCTGGGGGGTACGTGTGAGCTTGGCTAGATTAACTTCATACCTTCATATAATCTATCTATTAAGATGCAAAGTGGTATACAGTATTCTCAGTGGAAACATCCCTCTTATCTCCCTTTCAAACTAGAAAGGTGCAAGTTGATGTAATTTGTTTGCTGTAGTATATAGGGGAGGGACAAATACCTACTTTTTTTTCTTCCAAGTTTGTTAATTGAGAGTAATGTCTATTCTATTCATTCTCCATAAAGTCTTAGTGAAATAGACTGAAATTCTAAAGTTATTAGTGGATATCCAGTCCAGCAAAGGGGAGAATATGTCAAGAGCATCTCGTAGTGAGAAGAATTTGCATAATGAGGGAAACAGTGGAGGTAAATAAAGATGAAGGCCGGTTCGAGAATGTGCAACCGAAAGTTTGTGTCCAACACAATAGCTCTTTTGATAAAAACATGGAGGTAAAGTGAAAATTCCTGATTTCTCTTGTAATATCTCTGTGATAGAATTAATTGATTAGTAATTTGAATTGGTTAATGATCAATGTTCCTGAATTTTGATTATGCATCTTAGTAATATGGCAATAAACATTTATGCTCAATTGCACCTTGAAGTAGGTCATTAGTTAGTTGATGACATGAATGGAAATTGTTTAGTTTGGCAAACTTCAAAGGTGAACAGTTATGCTCTCATTTGCACCTAAAAATAGATCATTCAGTTAAGTGATGACATGAATGGATACTGTTTTCTTTGCCAACGTTCAACAGAAGAAAGTTTGATTAATAGATGACACAAAATACATGAATTTGTTCCCAAGTAATTTGAGCTCACTGTTTTTCTGTGGTGGACAATGGCAGGGCGATGGTAGTGCTGGAATGAAGAAAATTTTAACTATTGAGAATACTTTAAATCCGGAAAAGATTTTGCAGCCATACATGCAACATAAGGCACTTCATCATACTCTTGATCCGACCAAGTATAAGAAGACAGTGAACTGCAATCATGGAAGTCCTGTTTGGAATGGAGAAGCATGCTTAACATCATTGGACACTGATACAAGTGCAATGAGACCTACAAAACTGGTGCAATGA

Protein sequence

>MS.gene050156.t1

MQCGCGCVGAGGPAKGRICQEHLVVRRICIMRETVEVNKDEGRFENVQPKVCVQHNSSFDKNMEGDGSAGMKKILTIENTLNPEKILQPYMQHKALHHTLDPTKYKKTVNCNHGSPVWNGEACLTSLDTDTSAMRPTKLVQ