Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21247.t1 | XP_013467661.1 | 98.6 | 74 | 1 | 0 | 1 | 74 | 269 | 342 | 5.10E-30 | 139.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21247.t1 | A0A072VJS3 | 98.6 | 74 | 1 | 0 | 1 | 74 | 269 | 342 | 3.6e-30 | 139.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057932 | MS.gene21247 | 0.867939 | 9.45E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21247.t1 | MTR_1g053910 | 98.649 | 74 | 1 | 0 | 1 | 74 | 269 | 342 | 1.53e-44 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGATGCGAGCAAGGAAT+TGG | 0.376840 | 5.1:-52371591 | MS.gene21247:CDS |
| GGAGGAGGGAAAAGCTTTGA+GGG | 0.400312 | 5.1:-52371674 | MS.gene21247:CDS |
| TGGAGGAGGGAAAAGCTTTG+AGG | 0.441086 | 5.1:-52371675 | MS.gene21247:CDS |
| AGAAAGAATGAGAAGAAAAC+CGG | 0.442575 | 5.1:-52371650 | MS.gene21247:CDS |
| GGAATGCTGTGGAGGATCTT+CGG | 0.449412 | 5.1:-52371721 | MS.gene21247:CDS |
| GGAGAGAATTCACTAGTGTA+AGG | 0.470518 | 5.1:-52371700 | MS.gene21247:CDS |
| GAGGCAAATGGAGGTTCTGC+AGG | 0.475327 | 5.1:-52371762 | None:intergenic |
| GAGGTTCTGCAGGCGGAGAA+TGG | 0.475648 | 5.1:-52371752 | MS.gene21247:CDS |
| GGAATTGGAGAAGGCGTTGA+GGG | 0.502532 | 5.1:-52371576 | MS.gene21247:CDS |
| TTGAGGGGAGCCACCACTGT+TGG | 0.513960 | 5.1:-52371560 | MS.gene21247:CDS |
| TTCACTAGTGTAAGGATTGG+AGG | 0.529400 | 5.1:-52371692 | MS.gene21247:CDS |
| GAATTCACTAGTGTAAGGAT+TGG | 0.533076 | 5.1:-52371695 | MS.gene21247:CDS |
| GACTGAGGCTGATGCGAGCA+AGG | 0.549803 | 5.1:-52371597 | MS.gene21247:CDS |
| ACTAGTGTAAGGATTGGAGG+AGG | 0.570999 | 5.1:-52371689 | MS.gene21247:CDS |
| TCAGCCTCAGTCGATTTCCC+AGG | 0.596668 | 5.1:+52371608 | None:intergenic |
| GAGGAGGGAAAAGCTTTGAG+GGG | 0.601233 | 5.1:-52371673 | MS.gene21247:CDS |
| AATTCAAGCTCCAACAGTGG+TGG | 0.602493 | 5.1:+52371550 | None:intergenic |
| TTGAATTCAAGCTCCAACAG+TGG | 0.608904 | 5.1:+52371547 | None:intergenic |
| TGCGAGCAAGGAATTGGAGA+AGG | 0.616295 | 5.1:-52371585 | MS.gene21247:CDS |
| AGGAATTGGAGAAGGCGTTG+AGG | 0.624601 | 5.1:-52371577 | MS.gene21247:CDS |
| GCAAATGGAGGTTCTGCAGG+CGG | 0.640904 | 5.1:-52371759 | None:intergenic |
| GAAAGAATGAGAAGAAAACC+GGG | 0.648044 | 5.1:-52371649 | MS.gene21247:CDS |
| GAATTGGAGAAGGCGTTGAG+GGG | 0.650714 | 5.1:-52371575 | MS.gene21247:CDS |
| AGGCGGAGAATGGATTGCTG+AGG | 0.664151 | 5.1:-52371742 | MS.gene21247:CDS |
| ATTGCTGAGGAATGCTGTGG+AGG | 0.665643 | 5.1:-52371729 | MS.gene21247:CDS |
| CTAGTGTAAGGATTGGAGGA+GGG | 0.671920 | 5.1:-52371688 | MS.gene21247:CDS |
| TGGATTGCTGAGGAATGCTG+TGG | 0.678621 | 5.1:-52371732 | MS.gene21247:CDS |
| ATAACCACTAAAATCTGACC+CGG | 0.690957 | 5.1:+52371631 | None:intergenic |
| TTATCCTGGGAAATCGACTG+AGG | 0.704241 | 5.1:-52371612 | MS.gene21247:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAAAGAATGAGAAGAAAAC+CGG | - | chr5.1:52371658-52371677 | MS.gene21247:CDS | 30.0% | |
| ATAACCACTAAAATCTGACC+CGG | + | chr5.1:52371680-52371699 | None:intergenic | 35.0% | |
| GAAAGAATGAGAAGAAAACC+GGG | - | chr5.1:52371659-52371678 | MS.gene21247:CDS | 35.0% | |
| GAATTCACTAGTGTAAGGAT+TGG | - | chr5.1:52371613-52371632 | MS.gene21247:CDS | 35.0% | |
| !! | CAGATTTTAGTGGTTATCCT+GGG | - | chr5.1:52371683-52371702 | MS.gene21247:CDS | 35.0% |
| !! | TCAGATTTTAGTGGTTATCC+TGG | - | chr5.1:52371682-52371701 | MS.gene21247:CDS | 35.0% |
| GGAGAGAATTCACTAGTGTA+AGG | - | chr5.1:52371608-52371627 | MS.gene21247:CDS | 40.0% | |
| TTCACTAGTGTAAGGATTGG+AGG | - | chr5.1:52371616-52371635 | MS.gene21247:CDS | 40.0% | |
| ! | AAAACCGGGTCAGATTTTAG+TGG | - | chr5.1:52371673-52371692 | MS.gene21247:CDS | 40.0% |
| ACTAGTGTAAGGATTGGAGG+AGG | - | chr5.1:52371619-52371638 | MS.gene21247:CDS | 45.0% | |
| CTAGTGTAAGGATTGGAGGA+GGG | - | chr5.1:52371620-52371639 | MS.gene21247:CDS | 45.0% | |
| TTATCCTGGGAAATCGACTG+AGG | - | chr5.1:52371696-52371715 | MS.gene21247:CDS | 45.0% | |
| ATTGCTGAGGAATGCTGTGG+AGG | - | chr5.1:52371579-52371598 | MS.gene21247:CDS | 50.0% | |
| GGAATGCTGTGGAGGATCTT+CGG | - | chr5.1:52371587-52371606 | MS.gene21247:CDS | 50.0% | |
| TGCGAGCAAGGAATTGGAGA+AGG | - | chr5.1:52371723-52371742 | MS.gene21247:CDS | 50.0% | |
| TGGAGGAGGGAAAAGCTTTG+AGG | - | chr5.1:52371633-52371652 | MS.gene21247:CDS | 50.0% | |
| TGGATTGCTGAGGAATGCTG+TGG | - | chr5.1:52371576-52371595 | MS.gene21247:CDS | 50.0% | |
| ! | GGAGGAGGGAAAAGCTTTGA+GGG | - | chr5.1:52371634-52371653 | MS.gene21247:CDS | 50.0% |
| !! | AGGAATTGGAGAAGGCGTTG+AGG | - | chr5.1:52371731-52371750 | MS.gene21247:CDS | 50.0% |
| !! | GAATTGGAGAAGGCGTTGAG+GGG | - | chr5.1:52371733-52371752 | MS.gene21247:CDS | 50.0% |
| !! | GAGGAGGGAAAAGCTTTGAG+GGG | - | chr5.1:52371635-52371654 | MS.gene21247:CDS | 50.0% |
| !! | GGAATTGGAGAAGGCGTTGA+GGG | - | chr5.1:52371732-52371751 | MS.gene21247:CDS | 50.0% |
| GGCTGATGCGAGCAAGGAAT+TGG | - | chr5.1:52371717-52371736 | MS.gene21247:CDS | 55.0% | |
| ! | TCAGCCTCAGTCGATTTCCC+AGG | + | chr5.1:52371703-52371722 | None:intergenic | 55.0% |
| !! | AGGCGGAGAATGGATTGCTG+AGG | - | chr5.1:52371566-52371585 | MS.gene21247:CDS | 55.0% |
| GACTGAGGCTGATGCGAGCA+AGG | - | chr5.1:52371711-52371730 | MS.gene21247:CDS | 60.0% | |
| GAGGTTCTGCAGGCGGAGAA+TGG | - | chr5.1:52371556-52371575 | MS.gene21247:CDS | 60.0% | |
| TTGAGGGGAGCCACCACTGT+TGG | - | chr5.1:52371748-52371767 | MS.gene21247:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 52371553 | 52371777 | 52371553 | ID=MS.gene21247 |
| chr5.1 | mRNA | 52371553 | 52371777 | 52371553 | ID=MS.gene21247.t1;Parent=MS.gene21247 |
| chr5.1 | exon | 52371553 | 52371777 | 52371553 | ID=MS.gene21247.t1.exon1;Parent=MS.gene21247.t1 |
| chr5.1 | CDS | 52371553 | 52371777 | 52371553 | ID=cds.MS.gene21247.t1;Parent=MS.gene21247.t1 |
| Gene Sequence |
| Protein sequence |
