Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057932.t1 | XP_013467661.1 | 97.3 | 74 | 2 | 0 | 1 | 74 | 269 | 342 | 1.10E-29 | 138.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057932.t1 | A0A072VJS3 | 97.3 | 74 | 2 | 0 | 1 | 74 | 269 | 342 | 8.1e-30 | 138.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057932 | MS.gene068924 | 0.809719 | 1.65E-50 | -1.69E-46 |
| MS.gene057932 | MS.gene074202 | 0.805415 | 1.35E-49 | -1.69E-46 |
| MS.gene057932 | MS.gene21247 | 0.867939 | 9.45E-66 | -1.69E-46 |
| MS.gene057932 | MS.gene29920 | 0.804413 | 2.19E-49 | -1.69E-46 |
| MS.gene057932 | MS.gene29921 | 0.801895 | 7.25E-49 | -1.69E-46 |
| MS.gene057932 | MS.gene37071 | 0.818289 | 2.12E-52 | -1.69E-46 |
| MS.gene057932 | MS.gene48753 | 0.873392 | 1.52E-67 | -1.69E-46 |
| MS.gene057932 | MS.gene49793 | 0.830363 | 3.06E-55 | -1.69E-46 |
| MS.gene057932 | MS.gene49946 | 0.810025 | 1.41E-50 | -1.69E-46 |
| MS.gene057932 | MS.gene61413 | 0.819234 | 1.29E-52 | -1.69E-46 |
| MS.gene057932 | MS.gene68824 | 0.807626 | 4.61E-50 | -1.69E-46 |
| MS.gene057932 | MS.gene81845 | 0.912012 | 3.17E-83 | -1.69E-46 |
| MS.gene057932 | MS.gene87954 | 0.832079 | 1.16E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057932.t1 | MTR_1g053910 | 97.297 | 74 | 2 | 0 | 1 | 74 | 269 | 342 | 9.35e-44 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGATGCGAGCAAGGAAT+TGG | 0.376840 | 3.3:+14936373 | MS.gene057932:CDS |
| GGAGGAGGGAAAAGCTTTGA+GGG | 0.400312 | 3.3:+14936290 | MS.gene057932:CDS |
| TGGAGGAGGGAAAAGCTTTG+AGG | 0.441086 | 3.3:+14936289 | MS.gene057932:CDS |
| AGAAAGAATGAGAAGAAAAC+CGG | 0.442575 | 3.3:+14936314 | MS.gene057932:CDS |
| GGAATGCTGTGGAGGATCTT+CGG | 0.449412 | 3.3:+14936243 | MS.gene057932:CDS |
| GGAGAGAATTCACTAGTGTA+AGG | 0.470518 | 3.3:+14936264 | MS.gene057932:CDS |
| GAGGCAAATGGAGGTTCTGC+AGG | 0.475327 | 3.3:+14936202 | None:intergenic |
| GAGGTTCTGCAGGCGGAGAA+TGG | 0.475648 | 3.3:+14936212 | MS.gene057932:CDS |
| GGAATTGGAGAAGGCGTTGA+GGG | 0.502532 | 3.3:+14936388 | MS.gene057932:CDS |
| TTGAGGGGAGCCACCACTGT+TGG | 0.513960 | 3.3:+14936404 | MS.gene057932:CDS |
| TTCACTAGTGTAAGGATTGG+AGG | 0.529400 | 3.3:+14936272 | MS.gene057932:CDS |
| GAATTCACTAGTGTAAGGAT+TGG | 0.533076 | 3.3:+14936269 | MS.gene057932:CDS |
| GACTGAGGCTGATGCGAGCA+AGG | 0.549803 | 3.3:+14936367 | MS.gene057932:CDS |
| ACTAGTGTAAGGATTGGAGG+AGG | 0.570999 | 3.3:+14936275 | MS.gene057932:CDS |
| TCAGCCTCAGTCGATTTCCC+AGG | 0.596668 | 3.3:-14936356 | None:intergenic |
| GAGGAGGGAAAAGCTTTGAG+GGG | 0.601233 | 3.3:+14936291 | MS.gene057932:CDS |
| AATTCAAGCTCCAACAGTGG+TGG | 0.602493 | 3.3:-14936414 | None:intergenic |
| TTGAATTCAAGCTCCAACAG+TGG | 0.608904 | 3.3:-14936417 | None:intergenic |
| TGCGAGCAAGGAATTGGAGA+AGG | 0.616295 | 3.3:+14936379 | MS.gene057932:CDS |
| AGGAATTGGAGAAGGCGTTG+AGG | 0.624601 | 3.3:+14936387 | MS.gene057932:CDS |
| GCAAATGGAGGTTCTGCAGG+CGG | 0.640904 | 3.3:+14936205 | None:intergenic |
| GAAAGAATGAGAAGAAAACC+GGG | 0.648044 | 3.3:+14936315 | MS.gene057932:CDS |
| AGGCGGAGAATGGATTTCTG+AGG | 0.648560 | 3.3:+14936222 | MS.gene057932:CDS |
| GAATTGGAGAAGGCGTTGAG+GGG | 0.650714 | 3.3:+14936389 | MS.gene057932:CDS |
| ATTTCTGAGGAATGCTGTGG+AGG | 0.659127 | 3.3:+14936235 | MS.gene057932:CDS |
| CTAGTGTAAGGATTGGAGGA+GGG | 0.671920 | 3.3:+14936276 | MS.gene057932:CDS |
| TGGATTTCTGAGGAATGCTG+TGG | 0.678621 | 3.3:+14936232 | MS.gene057932:CDS |
| ATAACCACTAAAATCTGACC+CGG | 0.690957 | 3.3:-14936333 | None:intergenic |
| TTATCCTGGGAAATCGACTG+AGG | 0.704241 | 3.3:+14936352 | MS.gene057932:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAAAGAATGAGAAGAAAAC+CGG | + | chr3.3:14936314-14936333 | MS.gene057932:CDS | 30.0% | |
| ATAACCACTAAAATCTGACC+CGG | - | chr3.3:14936336-14936355 | None:intergenic | 35.0% | |
| GAAAGAATGAGAAGAAAACC+GGG | + | chr3.3:14936315-14936334 | MS.gene057932:CDS | 35.0% | |
| GAATTCACTAGTGTAAGGAT+TGG | + | chr3.3:14936269-14936288 | MS.gene057932:CDS | 35.0% | |
| !! | CAGATTTTAGTGGTTATCCT+GGG | + | chr3.3:14936339-14936358 | MS.gene057932:CDS | 35.0% |
| !! | TCAGATTTTAGTGGTTATCC+TGG | + | chr3.3:14936338-14936357 | MS.gene057932:CDS | 35.0% |
| GGAGAGAATTCACTAGTGTA+AGG | + | chr3.3:14936264-14936283 | MS.gene057932:CDS | 40.0% | |
| TTCACTAGTGTAAGGATTGG+AGG | + | chr3.3:14936272-14936291 | MS.gene057932:CDS | 40.0% | |
| ! | AAAACCGGGTCAGATTTTAG+TGG | + | chr3.3:14936329-14936348 | MS.gene057932:CDS | 40.0% |
| ACTAGTGTAAGGATTGGAGG+AGG | + | chr3.3:14936275-14936294 | MS.gene057932:CDS | 45.0% | |
| CTAGTGTAAGGATTGGAGGA+GGG | + | chr3.3:14936276-14936295 | MS.gene057932:CDS | 45.0% | |
| TTATCCTGGGAAATCGACTG+AGG | + | chr3.3:14936352-14936371 | MS.gene057932:CDS | 45.0% | |
| ! | ATTTCTGAGGAATGCTGTGG+AGG | + | chr3.3:14936235-14936254 | MS.gene057932:CDS | 45.0% |
| ! | TGGATTTCTGAGGAATGCTG+TGG | + | chr3.3:14936232-14936251 | MS.gene057932:CDS | 45.0% |
| GGAATGCTGTGGAGGATCTT+CGG | + | chr3.3:14936243-14936262 | MS.gene057932:CDS | 50.0% | |
| TGCGAGCAAGGAATTGGAGA+AGG | + | chr3.3:14936379-14936398 | MS.gene057932:CDS | 50.0% | |
| TGGAGGAGGGAAAAGCTTTG+AGG | + | chr3.3:14936289-14936308 | MS.gene057932:CDS | 50.0% | |
| ! | AGGCGGAGAATGGATTTCTG+AGG | + | chr3.3:14936222-14936241 | MS.gene057932:CDS | 50.0% |
| ! | GGAGGAGGGAAAAGCTTTGA+GGG | + | chr3.3:14936290-14936309 | MS.gene057932:CDS | 50.0% |
| !! | AGGAATTGGAGAAGGCGTTG+AGG | + | chr3.3:14936387-14936406 | MS.gene057932:CDS | 50.0% |
| !! | GAATTGGAGAAGGCGTTGAG+GGG | + | chr3.3:14936389-14936408 | MS.gene057932:CDS | 50.0% |
| !! | GAGGAGGGAAAAGCTTTGAG+GGG | + | chr3.3:14936291-14936310 | MS.gene057932:CDS | 50.0% |
| !! | GGAATTGGAGAAGGCGTTGA+GGG | + | chr3.3:14936388-14936407 | MS.gene057932:CDS | 50.0% |
| GGCTGATGCGAGCAAGGAAT+TGG | + | chr3.3:14936373-14936392 | MS.gene057932:CDS | 55.0% | |
| ! | TCAGCCTCAGTCGATTTCCC+AGG | - | chr3.3:14936359-14936378 | None:intergenic | 55.0% |
| GACTGAGGCTGATGCGAGCA+AGG | + | chr3.3:14936367-14936386 | MS.gene057932:CDS | 60.0% | |
| GAGGTTCTGCAGGCGGAGAA+TGG | + | chr3.3:14936212-14936231 | MS.gene057932:CDS | 60.0% | |
| TTGAGGGGAGCCACCACTGT+TGG | + | chr3.3:14936404-14936423 | MS.gene057932:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 14936209 | 14936433 | 14936209 | ID=MS.gene057932 |
| chr3.3 | mRNA | 14936209 | 14936433 | 14936209 | ID=MS.gene057932.t1;Parent=MS.gene057932 |
| chr3.3 | exon | 14936209 | 14936433 | 14936209 | ID=MS.gene057932.t1.exon1;Parent=MS.gene057932.t1 |
| chr3.3 | CDS | 14936209 | 14936433 | 14936209 | ID=cds.MS.gene057932.t1;Parent=MS.gene057932.t1 |
| Gene Sequence |
| Protein sequence |
