AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21393

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21393.t1 MTR_1g090657 94.975 398 17 2 1 396 1 397 0.0 758
MS.gene21393.t1 MTR_1g090657 91.709 398 14 3 1 396 1 381 0.0 722
MS.gene21393.t1 MTR_4g057530 39.175 97 56 1 298 394 206 299 6.31e-13 69.3
MS.gene21393.t1 MTR_4g057530 29.487 234 145 6 39 257 13 241 2.07e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21393.t1 AT5G04320 27.027 259 160 5 78 330 49 284 4.31e-12 67.8
MS.gene21393.t1 AT5G04320 27.027 259 160 5 78 330 49 284 4.31e-12 67.8
MS.gene21393.t1 AT5G04320 27.027 259 160 5 78 330 49 284 4.31e-12 67.8
MS.gene21393.t1 AT5G04320 26.820 261 162 5 76 330 23 260 4.62e-12 67.8
MS.gene21393.t1 AT3G44960 29.524 210 124 7 47 249 15 207 1.90e-11 64.7
MS.gene21393.t1 AT5G04320 26.378 254 158 5 83 330 3 233 4.75e-11 64.3
MS.gene21393.t1 AT3G44960 29.048 210 124 7 47 249 15 206 6.80e-11 62.8

Find 82 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTGGAAACTTCAAATTT+TGG 0.171801 1.4:-71744694 None:intergenic
TGACATATCATCACATAAAT+TGG 0.175167 1.4:-71744710 None:intergenic
TTCAGCTGGTTCTGGTTTCT+CGG 0.284010 1.4:-71744650 None:intergenic
CTGGTTTCTCGGGTCTGAAC+AGG 0.293350 1.4:-71744639 None:intergenic
CTCGCATCCTCTTGAAAGTT+TGG 0.293934 1.4:+71744273 MS.gene21393:CDS
GGTGATTCTGTTTCTGTTGT+TGG 0.311273 1.4:+71742827 MS.gene21393:CDS
AAGAAGTCTTCAGCTGGTTC+TGG 0.317331 1.4:-71744658 None:intergenic
GTGCTTTGGTTGGGCCTTGT+TGG 0.319039 1.4:-71742882 None:intergenic
CAACCTCCCGTCGCTGAAAC+TGG 0.327729 1.4:+71742469 MS.gene21393:CDS
TGCAGGGTGGTGCTTTGGTT+GGG 0.337227 1.4:-71742891 None:intergenic
GAGAAAGAATCTACATCATT+AGG 0.338286 1.4:+71744301 MS.gene21393:CDS
AAAACAAAGGAGAAGCAAAA+AGG 0.343577 1.4:+71743901 MS.gene21393:CDS
GTGCAGGGTGGTGCTTTGGT+TGG 0.351679 1.4:-71742892 None:intergenic
GGTGGAGGAAGTGGTGGTGC+AGG 0.351977 1.4:-71742908 None:intergenic
GGTGGTGCAGGGTGGTGCTT+TGG 0.372596 1.4:-71742896 None:intergenic
AGGCTAATTACATTGAGATT+TGG 0.379116 1.4:-71743934 None:intergenic
GCGTTGAAAGCTGGAAAATC+AGG 0.392933 1.4:+71744214 MS.gene21393:CDS
AGCTGCTCAGAAGAAAATTC+TGG 0.404402 1.4:+71742850 MS.gene21393:CDS
TCCGGCCACATCGTGAACTT+TGG 0.440039 1.4:-71744326 None:intergenic
TCAGCTGGTTCTGGTTTCTC+GGG 0.444940 1.4:-71744649 None:intergenic
CTGTGAAGGTTGTTTCGTAC+AGG 0.446552 1.4:+71744839 MS.gene21393:CDS
AAAGGAATGTTGAAAATAAG+AGG 0.462622 1.4:+71744477 MS.gene21393:CDS
GAAATGATGAGGAAGCTCTT+AGG 0.464073 1.4:+71743074 MS.gene21393:CDS
CAGCTGATCGACGCGAAGGC+CGG 0.476480 1.4:-71744819 None:intergenic
AAATCTGCAGCGTTGAAAGC+TGG 0.478212 1.4:+71744205 MS.gene21393:CDS
AAGATCGGAGTCGAGGAAGA+TGG 0.483738 1.4:-71742446 None:intergenic
TAAACAAGTCAAATCAGAAA+AGG 0.486455 1.4:+71744042 MS.gene21393:CDS
TCAGTTGAAGGTGGAGGAAG+TGG 0.486789 1.4:-71742917 None:intergenic
CAGAAAAGGTTGACAGTCAA+AGG 0.498447 1.4:+71744056 MS.gene21393:CDS
ATTACATTCAGTATCTTGTC+CGG 0.500303 1.4:-71744344 None:intergenic
TATAATATACCTTGAGAATG+AGG 0.502497 1.4:-71742952 None:intergenic
TGCCTGTACACTTGATCCTC+AGG 0.504464 1.4:+71744774 MS.gene21393:CDS
CCTTGTCCAGTTTCAGCGAC+GGG 0.512611 1.4:-71742475 None:intergenic
AAAACTTGTTCAGTGTTAGC+AGG 0.515952 1.4:-71744449 None:intergenic
GTGGAGGAAGTGGTGGTGCA+GGG 0.516422 1.4:-71742907 None:intergenic
GATTAGTGCAAGCAGACAAA+TGG 0.517988 1.4:+71743426 MS.gene21393:CDS
TTGACTTGTTTAACAACAGC+TGG 0.518722 1.4:-71744031 None:intergenic
AGCTATGAAATTAACATCGA+TGG 0.525803 1.4:+71743588 MS.gene21393:CDS
CAATTAATCTGAAAATGCGT+AGG 0.528526 1.4:+71744869 MS.gene21393:CDS
TGCTAATACTCAAATGATGC+GGG 0.529098 1.4:+71743294 MS.gene21393:CDS
GTAACACGAAGATCGTCTAT+TGG 0.529512 1.4:+71744796 MS.gene21393:CDS
TTCGCGTCGATCAGCTGTGA+AGG 0.535513 1.4:+71744825 MS.gene21393:CDS
AGATCGGAGTCGAGGAAGAT+GGG 0.546317 1.4:-71742445 None:intergenic
TCCAAAGTTCACGATGTGGC+CGG 0.550716 1.4:+71744325 MS.gene21393:CDS
TGGTTTCTCGGGTCTGAACA+GGG 0.557557 1.4:-71744638 None:intergenic
TCATTTGCCAAACTTTCAAG+AGG 0.559353 1.4:-71744280 None:intergenic
CACGAAGATCGTCTATTGGC+CGG 0.560924 1.4:+71744800 MS.gene21393:CDS
AATTAATCTGAAAATGCGTA+GGG 0.561635 1.4:+71744870 MS.gene21393:CDS
GTATCAAAGAAGTCTTCAGC+TGG 0.569283 1.4:-71744664 None:intergenic
TGTGAAGGTTGTTTCGTACA+GGG 0.572533 1.4:+71744840 MS.gene21393:CDS
ATTACAGGCATAGTTTGAGA+AGG 0.573967 1.4:+71744608 MS.gene21393:intron
GAGGAAGTGGTGGTGCAGGG+TGG 0.574962 1.4:-71742904 None:intergenic
CTCCGTTGACCTCATTCTCA+AGG 0.579673 1.4:+71742943 MS.gene21393:CDS
TCAACGGAGACATCAGTTGA+AGG 0.580490 1.4:-71742929 None:intergenic
TACCTGAGGATCAAGTGTAC+AGG 0.580964 1.4:-71744776 None:intergenic
CTCAGGAAAATGAAATGATG+AGG 0.584643 1.4:+71743063 MS.gene21393:intron
CAAACAGGTGTTCTTCGAGA+AGG 0.585970 1.4:+71744182 MS.gene21393:intron
CCATGGCATTGTTGCAGATG+AGG 0.589331 1.4:+71743734 MS.gene21393:CDS
TTCAGTGTTAGCAGGTCCCG+AGG 0.593990 1.4:-71744441 None:intergenic
CGAATCGCCACAACAATGGA+AGG 0.596720 1.4:+71742418 None:intergenic
TGAAGACTTCTTTGATACAG+AGG 0.602950 1.4:+71744669 MS.gene21393:CDS
GAAGTTTCGCTTGATGCTGT+AGG 0.604493 1.4:-71744742 None:intergenic
ACCTTGTCCAGTTTCAGCGA+CGG 0.605270 1.4:-71742476 None:intergenic
CCTCATCTGCAACAATGCCA+TGG 0.605304 1.4:-71743734 None:intergenic
TTCACAGCTGATCGACGCGA+AGG 0.610564 1.4:-71744823 None:intergenic
AACAGAGCAAAACTGATCCA+TGG 0.616879 1.4:+71743717 MS.gene21393:CDS
AAAGTTTGAAGAAGAAAACA+AGG 0.617125 1.4:+71743869 MS.gene21393:CDS
TTGCTAATACTCAAATGATG+CGG 0.619087 1.4:+71743293 MS.gene21393:CDS
GAGACATCAGTTGAAGGTGG+AGG 0.623469 1.4:-71742923 None:intergenic
AAGGGAGAAGAACAAAACAA+AGG 0.623499 1.4:+71743888 MS.gene21393:CDS
GAGGTTGAAGATCGGAGTCG+AGG 0.623839 1.4:-71742453 None:intergenic
AGCGACGGGAGGTTGAAGAT+CGG 0.630621 1.4:-71742461 None:intergenic
TGTCCAGTTTCAGCGACGGG+AGG 0.630744 1.4:-71742472 None:intergenic
CAAACTTTCAAGAGGATGCG+AGG 0.631072 1.4:-71744272 None:intergenic
GACGATCTTCGTGTTACCTG+AGG 0.646396 1.4:-71744790 None:intergenic
TACCTTGAGAATGAGGTCAA+CGG 0.647194 1.4:-71742945 None:intergenic
ACGGAGACATCAGTTGAAGG+TGG 0.656398 1.4:-71742926 None:intergenic
CCCGTCGCTGAAACTGGACA+AGG 0.671344 1.4:+71742475 MS.gene21393:CDS
GTTGAAGGTGGAGGAAGTGG+TGG 0.682513 1.4:-71742914 None:intergenic
AGGATCCAAAGTTCACGATG+TGG 0.700483 1.4:+71744321 MS.gene21393:CDS
TCTGGCTGATATAACCAACA+AGG 0.712159 1.4:+71742868 MS.gene21393:CDS
AAGTTTGAAGAAGAAAACAA+GGG 0.730503 1.4:+71743870 MS.gene21393:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAAAAAAAAGATAAA+TGG - chr1.4:71744376-71744395 None:intergenic 10.0%
!!! ATATATATATTTTCCTTTAT+TGG + chr1.4:71743764-71743783 MS.gene21393:intron 10.0%
!! AATACTATAACAACATTATT+GGG - chr1.4:71742982-71743001 None:intergenic 15.0%
!! TAATACTATAACAACATTAT+TGG - chr1.4:71742983-71743002 None:intergenic 15.0%
!!! TTGATATTCAAATCTAAAAT+TGG + chr1.4:71743378-71743397 MS.gene21393:intron 15.0%
!! TCGTATAGTAATTGAAAAAT+GGG + chr1.4:71742756-71742775 MS.gene21393:intron 20.0%
!! TTCGTATAGTAATTGAAAAA+TGG + chr1.4:71742755-71742774 MS.gene21393:intron 20.0%
!!! AAAAAGAGGTAAAATTTTGA+AGG + chr1.4:71742805-71742824 MS.gene21393:CDS 20.0%
!!! AAAAGAGGTAAAATTTTGAA+GGG + chr1.4:71742806-71742825 MS.gene21393:CDS 20.0%
!!! AAAGCAATTTTCAGAAATTT+CGG + chr1.4:71743248-71743267 MS.gene21393:CDS 20.0%
!!! AAATTGGAAACTTCAAATTT+TGG - chr1.4:71744697-71744716 None:intergenic 20.0%
!!! ATAGATAAACCTTTCAATTT+TGG - chr1.4:71742705-71742724 None:intergenic 20.0%
!!! TACATTGATTGAAGATAAAA+AGG - chr1.4:71743148-71743167 None:intergenic 20.0%
!!! TTCAAAATTTTACCTCTTTT+TGG - chr1.4:71742806-71742825 None:intergenic 20.0%
!!! TTTTTATCTTCAATCAATGT+AGG + chr1.4:71743147-71743166 MS.gene21393:intron 20.0%
!!! TTTTTTTGAATTTGTTTCTC+AGG + chr1.4:71743046-71743065 MS.gene21393:intron 20.0%
! AAAGGAATGTTGAAAATAAG+AGG + chr1.4:71744477-71744496 MS.gene21393:CDS 25.0%
! AAAGTTTGAAGAAGAAAACA+AGG + chr1.4:71743869-71743888 MS.gene21393:CDS 25.0%
! AAGTTTGAAGAAGAAAACAA+GGG + chr1.4:71743870-71743889 MS.gene21393:CDS 25.0%
! AATTAATCTGAAAATGCGTA+GGG + chr1.4:71744870-71744889 MS.gene21393:CDS 25.0%
! AGGATAGAAACAAAAGAAAA+AGG - chr1.4:71744398-71744417 None:intergenic 25.0%
! CAAGCAAAAAATTATGTTGA+AGG - chr1.4:71744418-71744437 None:intergenic 25.0%
! TAAACAAGTCAAATCAGAAA+AGG + chr1.4:71744042-71744061 MS.gene21393:CDS 25.0%
! TATAATATACCTTGAGAATG+AGG - chr1.4:71742955-71742974 None:intergenic 25.0%
! TGACATATCATCACATAAAT+TGG - chr1.4:71744713-71744732 None:intergenic 25.0%
!! ATTAAATTTTGTAGACAGAG+TGG - chr1.4:71743967-71743986 None:intergenic 25.0%
!! CTATATATATGCAGACATTT+TGG + chr1.4:71742721-71742740 MS.gene21393:intron 25.0%
!! TTAAATTTTGTAGACAGAGT+GGG - chr1.4:71743966-71743985 None:intergenic 25.0%
!!! GGAATAGTTTTTGTTTACTT+TGG + chr1.4:71743168-71743187 MS.gene21393:intron 25.0%
!!! TTAATGTCGAATTCTAAAGT+TGG + chr1.4:71744109-71744128 MS.gene21393:intron 25.0%
AAAACAAAGGAGAAGCAAAA+AGG + chr1.4:71743901-71743920 MS.gene21393:CDS 30.0%
AAACCATCAAAAACTAAAGC+AGG - chr1.4:71743668-71743687 None:intergenic 30.0%
AAATAAGAAATGAAAGCAGC+AGG - chr1.4:71743482-71743501 None:intergenic 30.0%
AACCATCAAAAACTAAAGCA+GGG - chr1.4:71743667-71743686 None:intergenic 30.0%
AGCTATGAAATTAACATCGA+TGG + chr1.4:71743588-71743607 MS.gene21393:CDS 30.0%
AGGCTAATTACATTGAGATT+TGG - chr1.4:71743937-71743956 None:intergenic 30.0%
AGTGAAATTCAGAATTGAAC+AGG - chr1.4:71742604-71742623 None:intergenic 30.0%
ATTACATTCAGTATCTTGTC+CGG - chr1.4:71744347-71744366 None:intergenic 30.0%
CAATTAATCTGAAAATGCGT+AGG + chr1.4:71744869-71744888 MS.gene21393:CDS 30.0%
CATTTACAAATTCATCAGGT+TGG - chr1.4:71743630-71743649 None:intergenic 30.0%
CGACATTAATCATGTGTAAA+AGG - chr1.4:71744098-71744117 None:intergenic 30.0%
GAGAAAGAATCTACATCATT+AGG + chr1.4:71744301-71744320 MS.gene21393:CDS 30.0%
TATGAATTCCACTTTCTGAA+TGG + chr1.4:71744512-71744531 MS.gene21393:intron 30.0%
TGCTCATTTACAAATTCATC+AGG - chr1.4:71743634-71743653 None:intergenic 30.0%
TGGATTCGAATGAATGAAAT+AGG + chr1.4:71744532-71744551 MS.gene21393:intron 30.0%
TGTCTTCACGAAAAAATTAC+AGG + chr1.4:71744593-71744612 MS.gene21393:intron 30.0%
TTGCTAATACTCAAATGATG+CGG + chr1.4:71743293-71743312 MS.gene21393:CDS 30.0%
! CTACGCATTTTCAGATTAAT+TGG - chr1.4:71744871-71744890 None:intergenic 30.0%
!!! GCAGACATTTTGGTTTTTTA+GGG + chr1.4:71742731-71742750 MS.gene21393:intron 30.0%
!!! TGCAGACATTTTGGTTTTTT+AGG + chr1.4:71742730-71742749 MS.gene21393:intron 30.0%
AAGGGAGAAGAACAAAACAA+AGG + chr1.4:71743888-71743907 MS.gene21393:CDS 35.0%
ACACCTGTTTGCATATTTGA+AGG - chr1.4:71744173-71744192 None:intergenic 35.0%
ACTGACACTCCAAAATTGAA+AGG + chr1.4:71742693-71742712 MS.gene21393:intron 35.0%
ATTACAGGCATAGTTTGAGA+AGG + chr1.4:71744608-71744627 MS.gene21393:intron 35.0%
ATTCGAATCCATTCAGAAAG+TGG - chr1.4:71744523-71744542 None:intergenic 35.0%
CTCAGGAAAATGAAATGATG+AGG + chr1.4:71743063-71743082 MS.gene21393:intron 35.0%
CTTGAACTAGCATGCAAAAA+TGG + chr1.4:71743556-71743575 MS.gene21393:CDS 35.0%
GAAATGAAAGAGAAGAAGAG+TGG - chr1.4:71742514-71742533 None:intergenic 35.0%
GATGATTATCACTACACTCA+TGG + chr1.4:71743022-71743041 MS.gene21393:intron 35.0%
TCATTTGCCAAACTTTCAAG+AGG - chr1.4:71744283-71744302 None:intergenic 35.0%
TCTCCTTCAAATATGCAAAC+AGG + chr1.4:71744167-71744186 MS.gene21393:intron 35.0%
TGAAGACTTCTTTGATACAG+AGG + chr1.4:71744669-71744688 MS.gene21393:CDS 35.0%
TGCTAATACTCAAATGATGC+GGG + chr1.4:71743294-71743313 MS.gene21393:CDS 35.0%
TTGAACAGGAAAACCCTAAT+CGG - chr1.4:71742590-71742609 None:intergenic 35.0%
TTGATTAAGTGAAAGTGAGC+TGG - chr1.4:71743816-71743835 None:intergenic 35.0%
! AAAACTTGTTCAGTGTTAGC+AGG - chr1.4:71744452-71744471 None:intergenic 35.0%
! CTGAACAAGTTTTTGCTGAA+AGG + chr1.4:71744459-71744478 MS.gene21393:CDS 35.0%
! GATCTTTTTATGTGCAATGC+AGG + chr1.4:71743402-71743421 MS.gene21393:intron 35.0%
! GGTTTGTTCTGTTTATAGTG+TGG + chr1.4:71743189-71743208 MS.gene21393:intron 35.0%
! TGACTGCTTACTCTAAGAAA+AGG - chr1.4:71743844-71743863 None:intergenic 35.0%
! TTTTCGTGAAGACATGAAAC+AGG - chr1.4:71744587-71744606 None:intergenic 35.0%
!! CATTCAATTTGTTGACATCC+AGG + chr1.4:71743519-71743538 MS.gene21393:intron 35.0%
!! GGTTTTGTCTTTTGATTCGT+AGG + chr1.4:71743683-71743702 MS.gene21393:intron 35.0%
!! TTGACTTGTTTAACAACAGC+TGG - chr1.4:71744034-71744053 None:intergenic 35.0%
!!! TAATTTTTTGCTTGCAGCCT+CGG + chr1.4:71744424-71744443 MS.gene21393:intron 35.0%
!!! TTTTTGGTTTTTGACCTGCA+AGG - chr1.4:71742790-71742809 None:intergenic 35.0%
AACAGAGCAAAACTGATCCA+TGG + chr1.4:71743717-71743736 MS.gene21393:CDS 40.0%
AAGGAGAAGCAAAAAGGAAG+AGG + chr1.4:71743907-71743926 MS.gene21393:CDS 40.0%
AGCTGCTCAGAAGAAAATTC+TGG + chr1.4:71742850-71742869 MS.gene21393:CDS 40.0%
CAAGCAGAAGAAGCCAATAA+AGG - chr1.4:71743780-71743799 None:intergenic 40.0%
CAGAAAAGGTTGACAGTCAA+AGG + chr1.4:71744056-71744075 MS.gene21393:CDS 40.0%
GAAATGATGAGGAAGCTCTT+AGG + chr1.4:71743074-71743093 MS.gene21393:CDS 40.0%
GATTAGTGCAAGCAGACAAA+TGG + chr1.4:71743426-71743445 MS.gene21393:CDS 40.0%
GCAGGTCAAAAACCAAAAAG+AGG + chr1.4:71742791-71742810 MS.gene21393:intron 40.0%
GGTTATGGGAATGAAGGATT+GGG - chr1.4:71742536-71742555 None:intergenic 40.0%
GTAACACGAAGATCGTCTAT+TGG + chr1.4:71744796-71744815 MS.gene21393:CDS 40.0%
GTATCAAAGAAGTCTTCAGC+TGG - chr1.4:71744667-71744686 None:intergenic 40.0%
TACCTTGAGAATGAGGTCAA+CGG - chr1.4:71742948-71742967 None:intergenic 40.0%
TCTGGCTGATATAACCAACA+AGG + chr1.4:71742868-71742887 MS.gene21393:CDS 40.0%
TGGTGAGTCTTGAAAACCAA+TGG + chr1.4:71743446-71743465 MS.gene21393:intron 40.0%
! AATGGGTTTCATTTCCTTGC+AGG + chr1.4:71742773-71742792 MS.gene21393:intron 40.0%
! ATTTGTTGACATCCAGGTGA+GGG + chr1.4:71743525-71743544 MS.gene21393:intron 40.0%
! TGTGAAGGTTGTTTCGTACA+GGG + chr1.4:71744840-71744859 MS.gene21393:CDS 40.0%
!! AATTTGTTGACATCCAGGTG+AGG + chr1.4:71743524-71743543 MS.gene21393:intron 40.0%
!! CGAATTCTAAAGTTGGACAC+CGG + chr1.4:71744116-71744135 MS.gene21393:intron 40.0%
!! GAATTCTAAAGTTGGACACC+GGG + chr1.4:71744117-71744136 MS.gene21393:intron 40.0%
!! GGTGATTCTGTTTCTGTTGT+TGG + chr1.4:71742827-71742846 MS.gene21393:CDS 40.0%
!! TATTGGCTTCTTCTGCTTGT+TGG + chr1.4:71743781-71743800 MS.gene21393:intron 40.0%
!! TTTTGTAGACAGAGTGGGTT+CGG - chr1.4:71743961-71743980 None:intergenic 40.0%
!!! AATTTTTTGCTTGCAGCCTC+GGG + chr1.4:71744425-71744444 MS.gene21393:intron 40.0%
!!! AGCCCTGCTTTAGTTTTTGA+TGG + chr1.4:71743662-71743681 MS.gene21393:intron 40.0%
AAAACCCTAATCGGAGTGAG+GGG - chr1.4:71742581-71742600 None:intergenic 45.0%
AGAGGGATGAGTAAACCCTA+GGG - chr1.4:71742558-71742577 None:intergenic 45.0%
AGGATCCAAAGTTCACGATG+TGG + chr1.4:71744321-71744340 MS.gene21393:CDS 45.0%
CAAACAGGTGTTCTTCGAGA+AGG + chr1.4:71744182-71744201 MS.gene21393:intron 45.0%
CAAACTTTCAAGAGGATGCG+AGG - chr1.4:71744275-71744294 None:intergenic 45.0%
CTCGCATCCTCTTGAAAGTT+TGG + chr1.4:71744273-71744292 MS.gene21393:CDS 45.0%
CTTCATTCCCATAACCCCTA+GGG + chr1.4:71742540-71742559 MS.gene21393:intron 45.0%
GAAAACCCTAATCGGAGTGA+GGG - chr1.4:71742582-71742601 None:intergenic 45.0%
GAAGTTTCGCTTGATGCTGT+AGG - chr1.4:71744745-71744764 None:intergenic 45.0%
GAGTAAACCCTAGGGGTTAT+GGG - chr1.4:71742550-71742569 None:intergenic 45.0%
GCGTTGAAAGCTGGAAAATC+AGG + chr1.4:71744214-71744233 MS.gene21393:CDS 45.0%
GGGTTATGGGAATGAAGGAT+TGG - chr1.4:71742537-71742556 None:intergenic 45.0%
TACCTGAGGATCAAGTGTAC+AGG - chr1.4:71744779-71744798 None:intergenic 45.0%
TCAACGGAGACATCAGTTGA+AGG - chr1.4:71742932-71742951 None:intergenic 45.0%
TGAGTAAACCCTAGGGGTTA+TGG - chr1.4:71742551-71742570 None:intergenic 45.0%
! AAATCTGCAGCGTTGAAAGC+TGG + chr1.4:71744205-71744224 MS.gene21393:CDS 45.0%
! AAGAAGTCTTCAGCTGGTTC+TGG - chr1.4:71744661-71744680 None:intergenic 45.0%
! CTGTGAAGGTTGTTTCGTAC+AGG + chr1.4:71744839-71744858 MS.gene21393:CDS 45.0%
! GACAGTCAAAGGTATGAGCT+TGG + chr1.4:71744067-71744086 MS.gene21393:intron 45.0%
! TGCCTTGTTCCTAAGTATCC+CGG - chr1.4:71744138-71744157 None:intergenic 45.0%
!! TTCAGCTGGTTCTGGTTTCT+CGG - chr1.4:71744653-71744672 None:intergenic 45.0%
AAGATCGGAGTCGAGGAAGA+TGG - chr1.4:71742449-71742468 None:intergenic 50.0%
ACCTTGTCCAGTTTCAGCGA+CGG - chr1.4:71742479-71742498 None:intergenic 50.0%
ACGGAGACATCAGTTGAAGG+TGG - chr1.4:71742929-71742948 None:intergenic 50.0%
AGATCGGAGTCGAGGAAGAT+GGG - chr1.4:71742448-71742467 None:intergenic 50.0%
AGCAGGTGTCAATGCACCAT+TGG - chr1.4:71743465-71743484 None:intergenic 50.0%
AGTTGGACACCGGGATACTT+AGG + chr1.4:71744126-71744145 MS.gene21393:intron 50.0%
CACCGGGATACTTAGGAACA+AGG + chr1.4:71744133-71744152 MS.gene21393:intron 50.0%
CACGAAGATCGTCTATTGGC+CGG + chr1.4:71744800-71744819 MS.gene21393:CDS 50.0%
CCATGGCATTGTTGCAGATG+AGG + chr1.4:71743734-71743753 MS.gene21393:CDS 50.0%
CCTAGGGGTTATGGGAATGA+AGG - chr1.4:71742542-71742561 None:intergenic 50.0%
CCTCATCTGCAACAATGCCA+TGG - chr1.4:71743737-71743756 None:intergenic 50.0%
CCTTCATTCCCATAACCCCT+AGG + chr1.4:71742539-71742558 MS.gene21393:intron 50.0%
CTCCGTTGACCTCATTCTCA+AGG + chr1.4:71742943-71742962 MS.gene21393:CDS 50.0%
GACGATCTTCGTGTTACCTG+AGG - chr1.4:71744793-71744812 None:intergenic 50.0%
GAGACATCAGTTGAAGGTGG+AGG - chr1.4:71742926-71742945 None:intergenic 50.0%
GAGAGGGATGAGTAAACCCT+AGG - chr1.4:71742559-71742578 None:intergenic 50.0%
GAGGGATGAGTAAACCCTAG+GGG - chr1.4:71742557-71742576 None:intergenic 50.0%
GGAAAACCCTAATCGGAGTG+AGG - chr1.4:71742583-71742602 None:intergenic 50.0%
TCAGTTGAAGGTGGAGGAAG+TGG - chr1.4:71742920-71742939 None:intergenic 50.0%
TCCAAAGTTCACGATGTGGC+CGG + chr1.4:71744325-71744344 MS.gene21393:CDS 50.0%
TGCCTGTACACTTGATCCTC+AGG + chr1.4:71744774-71744793 MS.gene21393:CDS 50.0%
TGGTTTCTCGGGTCTGAACA+GGG - chr1.4:71744641-71744660 None:intergenic 50.0%
!! TCAGCTGGTTCTGGTTTCTC+GGG - chr1.4:71744652-71744671 None:intergenic 50.0%
AGCGACGGGAGGTTGAAGAT+CGG - chr1.4:71742464-71742483 None:intergenic 55.0%
CAAGCTGAAGTTCCCTCACC+TGG - chr1.4:71743540-71743559 None:intergenic 55.0%
CCTTGTCCAGTTTCAGCGAC+GGG - chr1.4:71742478-71742497 None:intergenic 55.0%
CTAATCGGAGTGAGGGGAGA+GGG - chr1.4:71742575-71742594 None:intergenic 55.0%
CTCTCCCCTCACTCCGATTA+GGG + chr1.4:71742574-71742593 MS.gene21393:intron 55.0%
CTGGTTTCTCGGGTCTGAAC+AGG - chr1.4:71744642-71744661 None:intergenic 55.0%
GAGGTTGAAGATCGGAGTCG+AGG - chr1.4:71742456-71742475 None:intergenic 55.0%
GTTGAAGGTGGAGGAAGTGG+TGG - chr1.4:71742917-71742936 None:intergenic 55.0%
TCCGGCCACATCGTGAACTT+TGG - chr1.4:71744329-71744348 None:intergenic 55.0%
TGCAGGGTGGTGCTTTGGTT+GGG - chr1.4:71742894-71742913 None:intergenic 55.0%
TTCACAGCTGATCGACGCGA+AGG - chr1.4:71744826-71744845 None:intergenic 55.0%
TTCAGTGTTAGCAGGTCCCG+AGG - chr1.4:71744444-71744463 None:intergenic 55.0%
! GTGCTTTGGTTGGGCCTTGT+TGG - chr1.4:71742885-71742904 None:intergenic 55.0%
! TTCGCGTCGATCAGCTGTGA+AGG + chr1.4:71744825-71744844 MS.gene21393:CDS 55.0%
CAACCTCCCGTCGCTGAAAC+TGG + chr1.4:71742469-71742488 MS.gene21393:CDS 60.0%
CCCGTCGCTGAAACTGGACA+AGG + chr1.4:71742475-71742494 MS.gene21393:CDS 60.0%
CCTAATCGGAGTGAGGGGAG+AGG - chr1.4:71742576-71742595 None:intergenic 60.0%
CCTCTCCCCTCACTCCGATT+AGG + chr1.4:71742573-71742592 MS.gene21393:intron 60.0%
GTGCAGGGTGGTGCTTTGGT+TGG - chr1.4:71742895-71742914 None:intergenic 60.0%
TGTCCAGTTTCAGCGACGGG+AGG - chr1.4:71742475-71742494 None:intergenic 60.0%
! GTAGACAGAGTGGGTTCGGC+AGG - chr1.4:71743957-71743976 None:intergenic 60.0%
! GTGGAGGAAGTGGTGGTGCA+GGG - chr1.4:71742910-71742929 None:intergenic 60.0%
CAGCTGATCGACGCGAAGGC+CGG - chr1.4:71744822-71744841 None:intergenic 65.0%
GGTGGTGCAGGGTGGTGCTT+TGG - chr1.4:71742899-71742918 None:intergenic 65.0%
! GAGGAAGTGGTGGTGCAGGG+TGG - chr1.4:71742907-71742926 None:intergenic 65.0%
! GGTGGAGGAAGTGGTGGTGC+AGG - chr1.4:71742911-71742930 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 71742433 71744900 71742433 ID=MS.gene21393
chr1.4 mRNA 71742433 71744900 71742433 ID=MS.gene21393.t1;Parent=MS.gene21393
chr1.4 exon 71742433 71742496 71742433 ID=MS.gene21393.t1.exon1;Parent=MS.gene21393.t1
chr1.4 CDS 71742433 71742496 71742433 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71742795 71742964 71742795 ID=MS.gene21393.t1.exon2;Parent=MS.gene21393.t1
chr1.4 CDS 71742795 71742964 71742795 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71743068 71743105 71743068 ID=MS.gene21393.t1.exon3;Parent=MS.gene21393.t1
chr1.4 CDS 71743068 71743105 71743068 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71743207 71743315 71743207 ID=MS.gene21393.t1.exon4;Parent=MS.gene21393.t1
chr1.4 CDS 71743207 71743315 71743207 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71743424 71743447 71743424 ID=MS.gene21393.t1.exon5;Parent=MS.gene21393.t1
chr1.4 CDS 71743424 71743447 71743424 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71743541 71743609 71743541 ID=MS.gene21393.t1.exon6;Parent=MS.gene21393.t1
chr1.4 CDS 71743541 71743609 71743541 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71743705 71743755 71743705 ID=MS.gene21393.t1.exon7;Parent=MS.gene21393.t1
chr1.4 CDS 71743705 71743755 71743705 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71743852 71743945 71743852 ID=MS.gene21393.t1.exon8;Parent=MS.gene21393.t1
chr1.4 CDS 71743852 71743945 71743852 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71744023 71744077 71744023 ID=MS.gene21393.t1.exon9;Parent=MS.gene21393.t1
chr1.4 CDS 71744023 71744077 71744023 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71744189 71744361 71744189 ID=MS.gene21393.t1.exon10;Parent=MS.gene21393.t1
chr1.4 CDS 71744189 71744361 71744189 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71744441 71744498 71744441 ID=MS.gene21393.t1.exon11;Parent=MS.gene21393.t1
chr1.4 CDS 71744441 71744498 71744441 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
chr1.4 exon 71744615 71744900 71744615 ID=MS.gene21393.t1.exon12;Parent=MS.gene21393.t1
chr1.4 CDS 71744615 71744900 71744615 ID=cds.MS.gene21393.t1;Parent=MS.gene21393.t1
Gene Sequence

>MS.gene21393

ATGGAAGGTGCTCCCATCTTCCTCGACTCCGATCTTCAACCTCCCGTCGCTGAAACTGGACAAGGTATCAATCTCTCTCCACTCTTCTTCTCTTTCATTTCCCAATCCTTCATTCCCATAACCCCTAGGGTTTACTCATCCCTCTCCCCTCACTCCGATTAGGGTTTTCCTGTTCAATTCTGAATTTCACTTCTGTTTTGAATTTGCAATTAAGTGTTTGATTTAACTTCAATTAAGTGATTACATAGCTATTAGATCTAACTGACACTCCAAAATTGAAAGGTTTATCTATATATATGCAGACATTTTGGTTTTTTAGGGTTTCGTATAGTAATTGAAAAATGGGTTTCATTTCCTTGCAGGTCAAAAACCAAAAAGAGGTAAAATTTTGAAGGGTGATTCTGTTTCTGTTGTTGGAGCTGCTCAGAAGAAAATTCTGGCTGATATAACCAACAAGGCCCAACCAAAGCACCACCCTGCACCACCACTTCCTCCACCTTCAACTGATGTCTCCGTTGACCTCATTCTCAAGGTATATTATATATACCCAATAATGTTGTTATAGTATTATGATTATTATTGATTGATTGATGATTATCACTACACTCATGGATTTTTTTGAATTTGTTTCTCAGGAAAATGAAATGATGAGGAAGCTCTTAGGTCAAAGAAAGTAATTTTTTTTTATCTTCATTTTGTGTCTAATTTTATGCCTTTTTATCTTCAATCAATGTAGGAATAGTTTTTGTTTACTTTGGTTTGTTCTGTTTATAGTGTGGTAATAGAATCATATAAAGCAGAGCTTCAGAAGTATCAAAGCAATTTTCAGAAATTTCGGAAGCAAAATGCAGAACTTGCACTTGCTAATACTCAAATGATGCGGGTACTACTGACTATATGTGTTTTAACTGTTGTTTATTTTAAGTTATTTGCTATTGTTGATTGTTGATATTCAAATCTAAAATTGGTGATCTTTTTATGTGCAATGCAGGAGATTAGTGCAAGCAGACAAATGGTGAGTCTTGAAAACCAATGGTGCATTGACACCTGCTGCTTTCATTTCTTATTTTTTGTTATGTTTAGTGACATTCAATTTGTTGACATCCAGGTGAGGGAACTTCAGCTTGAACTAGCATGCAAAAATGGTATACTCAAAGCTATGAAATTAACATCGATGGTTAGTTTAACTCATCTCCAACCTGATGAATTTGTAAATGAGCAATTCAGAGAGCCCTGCTTTAGTTTTTGATGGTTTTGTCTTTTGATTCGTAGGAAAATGATCACAACAGAGCAAAACTGATCCATGGCATTGTTGCAGATGAGGTACTTATATATATATATTTTCCTTTATTGGCTTCTTCTGCTTGTTGGTCTTTGCTTCCAGCTCACTTTCACTTAATCAATTTTTCCTTTTCTTAGAGTAAGCAGTCAGATCAAAAGTTTGAAGAAGAAAACAAGGGAGAAGAACAAAACAAAGGAGAAGCAAAAAGGAAGAGGATGTCCAAATCTCAATGTAATTAGCCTGCCGAACCCACTCTGTCTACAAAATTTAATTTATATATCTTTTAAGTAGATATTTCTCTCATGCAGCTTCTGCACCAGCTGTTGTTAAACAAGTCAAATCAGAAAAGGTTGACAGTCAAAGGTATGAGCTTGGATTTACCTTTTACACATGATTAATGTCGAATTCTAAAGTTGGACACCGGGATACTTAGGAACAAGGCAGCTTATATGTCTCCTTCAAATATGCAAACAGGTGTTCTTCGAGAAGGAAATCTGCAGCGTTGAAAGCTGGAAAATCAGGATCAACAGAAGAAGTATTTGAAATAAAATATGATGCCTCGCATCCTCTTGAAAGTTTGGCAAATGAGAAAGAATCTACATCATTAGGATCCAAAGTTCACGATGTGGCCGGACAAGATACTGAATGTAATTGCATGCCATTTATCTTTTTTTTTTTTTCCTTTTTCTTTTGTTTCTATCCTTCAACATAATTTTTTGCTTGCAGCCTCGGGACCTGCTAACACTGAACAAGTTTTTGCTGAAAGGAATGTTGAAAATAAGAGGTATACTATTATATATGAATTCCACTTTCTGAATGGATTCGAATGAATGAAATAGGAAAATGTGATTAATTTAGATAATTGAATGCCTGTTTCATGTCTTCACGAAAAAATTACAGGCATAGTTTGAGAAGGCAATCTGCCCTGTTCAGACCCGAGAAACCAGAACCAGCTGAAGACTTCTTTGATACAGAGGATCCAAAATTTGAAGTTTCCAATTTATGTGATGATATGTCAGAAAGTCTTCCTACAGCATCAAGCGAAACTTCAGAAAACAATGCCTGTACACTTGATCCTCAGGTAACACGAAGATCGTCTATTGGCCGGCCTTCGCGTCGATCAGCTGTGAAGGTTGTTTCGTACAGGGAAGTTCCAATTAATCTGAAAATGCGTAGGGATAAGTGA
Protein sequence

>MS.gene21393.t1

MEGAPIFLDSDLQPPVAETGQGQKPKRGKILKGDSVSVVGAAQKKILADITNKAQPKHHPAPPLPPPSTDVSVDLILKENEMMRKLLGQRNVVIESYKAELQKYQSNFQKFRKQNAELALANTQMMREISASRQMVRELQLELACKNGILKAMKLTSMENDHNRAKLIHGIVADESKQSDQKFEEENKGEEQNKGEAKRKRMSKSQSSAPAVVKQVKSEKVDSQRCSSRRKSAALKAGKSGSTEEVFEIKYDASHPLESLANEKESTSLGSKVHDVAGQDTESSGPANTEQVFAERNVENKRHSLRRQSALFRPEKPEPAEDFFDTEDPKFEVSNLCDDMSESLPTASSETSENNACTLDPQVTRRSSIGRPSRRSAVKVVSYREVPINLKMRRDK