AlfalfaGEDB | MS.gene21546

M. sativa cultivar XinJiangDaYe / MS.gene21546

KEGG: K14490
GO: GO:0000160 GO:0007165 GO:0008150 GO:0009987 GO:0035556 GO:0050789 GO:0050794 GO:0065007
PFAM: PF01627.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21546.t1	4G78_A	100	109	0	0	1	109	4	112	1.90E-54	221.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21546.t1	Q9ZNV9	54.4	103	47	0	6	108	8	110	2.7e-28	125.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21546.t1	G7I2T8	100.0	109	0	0	1	109	1	109	1.4e-54	221.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052040	MS.gene21546	0.811216	7.82E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21546.t1	MTR_1g089130	100.000	109	0	0	1	109	1	109	5.71e-79	229
MS.gene21546.t1	MTR_1g082290	50.459	109	54	0	1	109	4	112	1.02e-39	129
MS.gene21546.t1	MTR_7g114020	52.885	104	48	1	7	109	6	109	2.01e-38	126
MS.gene21546.t1	MTR_2g020770	54.206	107	49	0	3	109	5	111	1.01e-34	116
MS.gene21546.t1	MTR_2g020770	54.206	107	49	0	3	109	5	111	1.88e-34	116
MS.gene21546.t1	MTR_2g100900	41.905	105	61	0	5	109	7	111	1.84e-28	101
MS.gene21546.t1	MTR_2g100880	41.905	105	61	0	5	109	7	111	1.86e-28	100
MS.gene21546.t1	MTR_2g103870	48.980	98	49	1	13	109	18	115	4.64e-19	77.4
MS.gene21546.t1	MTR_4g010160	50.515	97	45	2	15	109	8	103	9.80e-15	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21546.t1	AT3G29350	55.556	108	47	1	3	109	7	114	1.18e-41	133
MS.gene21546.t1	AT3G29350	55.556	108	47	1	3	109	7	114	4.35e-41	133
MS.gene21546.t1	AT1G03430	55.963	109	45	2	3	109	8	115	6.51e-40	130
MS.gene21546.t1	AT1G03430	55.963	109	45	2	3	109	8	115	1.16e-39	129
MS.gene21546.t1	AT5G39340	53.704	108	49	1	3	109	7	114	1.15e-38	127
MS.gene21546.t1	AT5G39340	53.704	108	49	1	3	109	40	147	2.52e-38	127
MS.gene21546.t1	AT5G39340	53.704	108	49	1	3	109	56	163	4.05e-38	127
MS.gene21546.t1	AT5G39340	53.704	108	49	1	3	109	56	163	4.05e-38	127
MS.gene21546.t1	AT3G16360	51.042	96	47	0	14	109	11	106	5.49e-33	112
MS.gene21546.t1	AT3G21510	53.333	105	49	0	5	109	7	111	8.24e-33	112
MS.gene21546.t1	AT3G16360	52.174	92	44	0	18	109	9	100	5.80e-32	109
MS.gene21546.t1	AT4G04402	45.122	82	35	2	19	90	24	105	2.28e-16	70.5
MS.gene21546.t1	AT1G80100	47.959	98	50	1	13	109	18	115	2.05e-14	65.1
MS.gene21546.t1	AT1G80100	44.545	110	60	1	1	109	6	115	2.30e-14	65.1

Find 11 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAAGAAAACTCACCCTTCC+AGG	0.303814	1.1:+65599926	None:intergenic
ATAACCTCAATCACAAAATC+AGG	0.417121	1.1:+65600663	None:intergenic
CGAAGCTGCTATGTTTCAAC+AGG	0.430572	1.1:-65600905	MS.gene21546:intron
TGCTCATGCCCATCAACAAA+AGG	0.448350	1.1:-65600123	MS.gene21546:CDS
TCTATTTGTTTAAAATTCAC+AGG	0.488923	1.1:+65600146	None:intergenic
GTTGAAACATAGCAGCTTCG+TGG	0.501753	1.1:+65600908	None:intergenic
TGCGAGGCTCAGAACCTGGA+AGG	0.534171	1.1:-65599940	MS.gene21546:CDS
GCTCATGCCCATCAACAAAA+GGG	0.561552	1.1:-65600122	MS.gene21546:CDS
GCGAGGCTCAGAACCTGGAA+GGG	0.598860	1.1:-65599939	MS.gene21546:CDS
AACACATGGATCACTTACAT+AGG	0.611392	1.1:-65600942	None:intergenic
AACAAAAGGGAAGCAGTGCT+AGG	0.617678	1.1:-65600109	MS.gene21546:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATAAGAATACAAAAAAAA+AGG	+	chr1.1:65600052-65600071	None:intergenic	10.0%
!!	AACATCTTAATCAATTAAAA+AGG	-	chr1.1:65600448-65600467	MS.gene21546:intron	15.0%
!!	TATTACTCTCTTTAATTAAT+TGG	-	chr1.1:65600301-65600320	MS.gene21546:intron	15.0%
!!!	AATTTGATATTATTGGTTAT+TGG	-	chr1.1:65600125-65600144	MS.gene21546:CDS	15.0%
!!!	ATTTGATATTATTGGTTATT+GGG	-	chr1.1:65600126-65600145	MS.gene21546:CDS	15.0%
!!	AATTATTGAATAATATGTGC+CGG	-	chr1.1:65600519-65600538	MS.gene21546:intron	20.0%
!!	TAAATAAAATCTACTTCCAT+AGG	+	chr1.1:65600840-65600859	None:intergenic	20.0%
!!	TAATAGTAATAGTACTTACA+GGG	+	chr1.1:65600286-65600305	None:intergenic	20.0%
!!!	ATTTATTTGTTAAACAGTGT+TGG	-	chr1.1:65600867-65600886	MS.gene21546:intron	20.0%
!!!	TCTATTTGTTTAAAATTCAC+AGG	+	chr1.1:65600730-65600749	None:intergenic	20.0%
!!!	TTTGATATTATTGGTTATTG+GGG	-	chr1.1:65600127-65600146	MS.gene21546:CDS	20.0%
!	ACATGTAACGAAAAGATTAT+AGG	+	chr1.1:65599996-65600015	None:intergenic	25.0%
!	ATTATTGAATAATATGTGCC+GGG	-	chr1.1:65600520-65600539	MS.gene21546:intron	25.0%
!	GTAATAGTAATAGTACTTAC+AGG	+	chr1.1:65600287-65600306	None:intergenic	25.0%
!!	TTGATATTATTGGTTATTGG+GGG	-	chr1.1:65600128-65600147	MS.gene21546:CDS	25.0%
	ATAACCTCAATCACAAAATC+AGG	+	chr1.1:65600213-65600232	None:intergenic	30.0%
	GATGCATGGAAATATTAAAC+TGG	-	chr1.1:65600672-65600691	MS.gene21546:CDS	30.0%
	GCTAGAGTTAAAAATGTTTG+TGG	-	chr1.1:65600894-65600913	MS.gene21546:intron	30.0%
	GTTATACCAAAAACTTTCGT+AGG	-	chr1.1:65600632-65600651	MS.gene21546:CDS	30.0%
!!!	CTTTTAGTTTGTTTGATGCA+TGG	-	chr1.1:65600658-65600677	MS.gene21546:CDS	30.0%
	TACATAGCACAAAAGTTGGA+GGG	-	chr1.1:65600557-65600576	MS.gene21546:intron	35.0%
	TGTATTAGTGCACTAACCTA+TGG	-	chr1.1:65600821-65600840	MS.gene21546:intron	35.0%
!	AAGTCACCTACGAAAGTTTT+TGG	+	chr1.1:65600641-65600660	None:intergenic	35.0%
!	TAGTCCTGATTTTGTGATTG+AGG	-	chr1.1:65600206-65600225	MS.gene21546:intron	35.0%
!!!	GAAAGTTTTTGGTATAACCC+AGG	+	chr1.1:65600630-65600649	None:intergenic	35.0%
	ACCCTAGGATACGACAATTT+GGG	+	chr1.1:65600601-65600620	None:intergenic	40.0%
	CTACATAGCACAAAAGTTGG+AGG	-	chr1.1:65600556-65600575	MS.gene21546:intron	40.0%
	GTGCTACATAGCACAAAAGT+TGG	-	chr1.1:65600553-65600572	MS.gene21546:intron	40.0%
	GTTAGTGTCAATATCGTGTC+TGG	+	chr1.1:65600387-65600406	None:intergenic	40.0%
	TACCCTAGGATACGACAATT+TGG	+	chr1.1:65600602-65600621	None:intergenic	40.0%
!	ACTGCTTCCCTTTTGTTGAT+GGG	+	chr1.1:65600761-65600780	None:intergenic	40.0%
!	CACGTTCAGAAATTTTTGCG+AGG	-	chr1.1:65600917-65600936	MS.gene21546:CDS	40.0%
	AACAAAAGGGAAGCAGTGCT+AGG	-	chr1.1:65600764-65600783	MS.gene21546:intron	45.0%
	CGAAGCTGCTATGTTTCAAC+AGG	-	chr1.1:65599968-65599987	MS.gene21546:CDS	45.0%
	CTAGGATACGACAATTTGGG+CGG	+	chr1.1:65600598-65600617	None:intergenic	45.0%
	GCCCAAATTGTCGTATCCTA+GGG	-	chr1.1:65600597-65600616	MS.gene21546:intron	45.0%
	GCTCATGCCCATCAACAAAA+GGG	-	chr1.1:65600751-65600770	MS.gene21546:intron	45.0%
	GTTGAAACATAGCAGCTTCG+TGG	+	chr1.1:65599968-65599987	None:intergenic	45.0%
	TATCCTAGGGTACGATACCT+GGG	-	chr1.1:65600610-65600629	MS.gene21546:CDS	45.0%
	TGCTCATGCCCATCAACAAA+AGG	-	chr1.1:65600750-65600769	MS.gene21546:intron	45.0%
!	CACTGCTTCCCTTTTGTTGA+TGG	+	chr1.1:65600762-65600781	None:intergenic	45.0%
!	TATTGGTTATTGGGGGAGCA+GGG	-	chr1.1:65600135-65600154	MS.gene21546:CDS	45.0%
!	TTATTGGTTATTGGGGGAGC+AGG	-	chr1.1:65600134-65600153	MS.gene21546:CDS	45.0%
!!	TTTAATAAATTTGATATTAT+TGG	-	chr1.1:65600118-65600137	MS.gene21546:CDS	5.0%
	CGCCCAAATTGTCGTATCCT+AGG	-	chr1.1:65600596-65600615	MS.gene21546:intron	50.0%
	GTATCCTAGGGTACGATACC+TGG	-	chr1.1:65600609-65600628	MS.gene21546:CDS	50.0%
!	TAACCCAGGTATCGTACCCT+AGG	+	chr1.1:65600616-65600635	None:intergenic	50.0%
!	TTTTTGCGAGGCTCAGAACC+TGG	-	chr1.1:65600929-65600948	MS.gene21546:CDS	50.0%
	ATTGGGGGAGCAGGGTTATC+TGG	-	chr1.1:65600143-65600162	MS.gene21546:CDS	55.0%
	GCGAGGCTCAGAACCTGGAA+GGG	-	chr1.1:65600934-65600953	MS.gene21546:CDS	60.0%
	TGCGAGGCTCAGAACCTGGA+AGG	-	chr1.1:65600933-65600952	MS.gene21546:CDS	60.0%
	TGTAGCACGTGTGCGACACC+CGG	+	chr1.1:65600541-65600560	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	65599936	65600959	65599936	ID=MS.gene21546
chr1.1	mRNA	65599936	65600959	65599936	ID=MS.gene21546.t1;Parent=MS.gene21546
chr1.1	exon	65600906	65600959	65600906	ID=MS.gene21546.t1.exon1;Parent=MS.gene21546.t1
chr1.1	CDS	65600906	65600959	65600906	ID=cds.MS.gene21546.t1;Parent=MS.gene21546.t1
chr1.1	exon	65600609	65600739	65600609	ID=MS.gene21546.t1.exon2;Parent=MS.gene21546.t1
chr1.1	CDS	65600609	65600739	65600609	ID=cds.MS.gene21546.t1;Parent=MS.gene21546.t1
chr1.1	exon	65600110	65600178	65600110	ID=MS.gene21546.t1.exon3;Parent=MS.gene21546.t1
chr1.1	CDS	65600110	65600178	65600110	ID=cds.MS.gene21546.t1;Parent=MS.gene21546.t1
chr1.1	exon	65599936	65600011	65599936	ID=MS.gene21546.t1.exon4;Parent=MS.gene21546.t1
chr1.1	CDS	65599936	65600011	65599936	ID=cds.MS.gene21546.t1;Parent=MS.gene21546.t1

Gene Sequence

>MS.gene21546

ATGGATCACTTACATAGGAAGCTACGCGACCACGAAGCTGCTATGTTTCAACAGGTTCCTATAATCTTTTCGTTACATGTTTTTCATAATTGTTTCATTCAAATATATAATTACCTTTTTTTTTGTATTCTTATTTTTGAGATGAAATCACGTCAAAAAAGCTTGATTATTGTTTTATTATTTTTAATAAATTTGATATTATTGGTTATTGGGGGAGCAGGGTTATCTGGATGATCAATTCAGTCAGCTTCAGAAACTGCAAGATGACACTAGTCCTGATTTTGTGATTGAGGTTATGACTATGTTCTTTGATGATTCTGAGAAACTTCTCAACAACATGTCACGTGCCCTGTAAGTACTATTACTATTACTCTCTTTAATTAATTGGATTTTATTTTCTTAGACTGTGAGATGACACATACAAACATATGAATCACATACAAAGACACCAGACACGATATTGACACTAACATGTTGACACAAATAATAATTTGAGAAAATAAAATACTTAAAACATCTTAATCAATTAAAAAGGCATCAGATACGACATTGACACTGACGTATTGACACATCTAATAATTTTAATTATTGAATAATATGTGCCGGGTGTCGCACACGTGCTACATAGCACAAAAGTTGGAGGGAGAGCGCGAGCTTTGCCGCCCAAATTGTCGTATCCTAGGGTACGATACCTGGGTTATACCAAAAACTTTCGTAGGTGACTTTTAGTTTGTTTGATGCATGGAAATATTAAACTGGTTCTATTTGATTATTTCTGTAGAGAGCAAGTACCTGTGAATTTTAAACAAATAGATGCTCATGCCCATCAACAAAAGGGAAGCAGTGCTAGGTATTAAAGCTGTATTTGTATTTCATGATCTGTAGTGTATTAGTGCACTAACCTATGGAAGTAGATTTTATTTATTTGTGAATTTATTTGTTAAACAGTGTTGGTGCTGCTAGAGTTAAAAATGTTTGTGGCACGTTCAGAAATTTTTGCGAGGCTCAGAACCTGGAAGGGTGA

Protein sequence

>MS.gene21546.t1

MDHLHRKLRDHEAAMFQQGYLDDQFSQLQKLQDDTSPDFVIEVMTMFFDDSEKLLNNMSRALEQVPVNFKQIDAHAHQQKGSSASVGAARVKNVCGTFRNFCEAQNLEG