AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21587


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21587.t1 MTR_1g090190 89.444 180 13 2 1 175 1 179 2.83e-107 304
MS.gene21587.t1 MTR_2g088990 38.519 135 75 4 3 136 9 136 3.73e-28 105
MS.gene21587.t1 MTR_3g099580 42.609 115 65 1 4 118 8 121 5.54e-28 103
MS.gene21587.t1 MTR_8g007035 40.517 116 61 2 4 117 11 120 4.08e-27 101
MS.gene21587.t1 MTR_3g099980 40.171 117 65 3 5 119 11 124 5.00e-26 99.4
MS.gene21587.t1 MTR_3g099680 40.171 117 65 3 5 119 11 124 7.51e-26 99.0
MS.gene21587.t1 MTR_8g007020 31.461 178 102 4 4 167 11 182 1.66e-25 97.1
MS.gene21587.t1 MTR_3g099570 34.752 141 83 2 5 145 11 142 2.29e-25 96.7
MS.gene21587.t1 MTR_6g080660 36.441 118 71 1 1 118 10 123 2.45e-24 94.7
MS.gene21587.t1 MTR_5g006040 31.056 161 103 3 1 153 4 164 1.15e-23 92.0
MS.gene21587.t1 MTR_3g099540 35.938 128 79 1 1 128 8 132 5.12e-22 86.7
MS.gene21587.t1 MTR_4g114870 42.574 101 53 2 22 117 190 290 7.47e-22 90.9
MS.gene21587.t1 MTR_4g114870 33.824 136 83 3 4 134 10 143 2.29e-16 75.9
MS.gene21587.t1 MTR_1g104800 34.167 120 75 3 1 117 4 122 5.66e-21 85.1
MS.gene21587.t1 MTR_6g013200 33.129 163 91 5 4 157 16 169 8.61e-21 84.3
MS.gene21587.t1 MTR_6g013420 38.235 136 78 4 4 136 19 151 9.07e-21 84.7
MS.gene21587.t1 MTR_6g023760 39.394 99 59 1 20 117 23 121 6.28e-20 82.4
MS.gene21587.t1 MTR_1g112700 34.058 138 79 4 4 130 14 150 2.33e-18 78.2
MS.gene21587.t1 MTR_2g025580 29.577 142 97 3 4 143 8 148 3.87e-18 77.4
MS.gene21587.t1 MTR_8g099220 35.938 128 69 4 2 118 3 128 4.82e-17 73.6
MS.gene21587.t1 MTR_8g463180 36.364 121 73 3 1 117 1 121 5.51e-17 77.0
MS.gene21587.t1 MTR_8g086360 33.813 139 84 4 4 135 6 143 7.18e-17 74.7
MS.gene21587.t1 MTR_0334s0010 34.127 126 75 2 1 118 1 126 1.18e-16 74.3
MS.gene21587.t1 MTR_6g022170 38.462 104 58 2 19 117 30 132 1.61e-16 73.2
MS.gene21587.t1 MTR_8g089110 36.842 114 68 3 4 116 9 119 4.76e-16 70.9
MS.gene21587.t1 MTR_1g014120 32.867 143 83 5 4 135 9 149 1.08e-15 71.2
MS.gene21587.t1 MTR_1g090420 30.263 152 96 4 5 152 10 155 4.14e-15 70.5
MS.gene21587.t1 MTR_4g130780 31.061 132 83 4 5 128 9 140 8.87e-15 68.9
MS.gene21587.t1 MTR_8g094990 32.593 135 82 4 4 130 7 140 1.39e-14 70.1
MS.gene21587.t1 MTR_4g130800 34.454 119 70 4 5 115 9 127 1.73e-14 69.7
MS.gene21587.t1 MTR_2g090575 33.577 137 80 4 4 130 7 142 1.84e-14 70.1
MS.gene21587.t1 MTR_6g083240 30.818 159 96 6 20 170 24 176 3.09e-14 67.4
MS.gene21587.t1 MTR_7g086100 29.268 123 84 2 2 121 5 127 5.49e-14 67.8
MS.gene21587.t1 MTR_3g089035 35.294 102 62 3 17 117 27 125 1.07e-13 64.7
MS.gene21587.t1 MTR_7g086090 29.310 116 81 1 4 118 7 122 1.78e-13 66.6
MS.gene21587.t1 MTR_4g124280 32.800 125 70 3 20 130 22 146 1.98e-13 66.2
MS.gene21587.t1 MTR_2g101300 29.787 141 89 4 9 142 11 148 2.41e-13 65.1
MS.gene21587.t1 MTR_1g077790 36.190 105 61 3 23 121 29 133 3.66e-13 64.7
MS.gene21587.t1 MTR_4g081100 32.710 107 67 2 19 120 28 134 4.13e-13 65.1
MS.gene21587.t1 MTR_7g086160 31.405 121 80 3 4 121 7 127 4.46e-13 63.9
MS.gene21587.t1 MTR_2g079030 33.333 132 83 4 2 130 5 134 4.94e-13 66.6
MS.gene21587.t1 MTR_1g105130 28.926 121 84 2 3 121 5 125 5.10e-13 63.5
MS.gene21587.t1 MTR_1g105120 28.926 121 84 2 3 121 5 125 5.10e-13 63.5
MS.gene21587.t1 MTR_2g043730 36.207 116 68 4 4 116 11 123 7.42e-13 62.4
MS.gene21587.t1 MTR_3g105930 33.628 113 67 3 26 130 31 143 8.79e-13 63.5
MS.gene21587.t1 MTR_4g077787 33.333 123 74 3 4 119 12 133 9.78e-13 63.5
MS.gene21587.t1 MTR_4g130800 36.364 99 57 3 23 115 28 126 1.26e-12 64.3
MS.gene21587.t1 MTR_4g078410 30.822 146 96 3 4 146 6 149 1.40e-12 63.2
MS.gene21587.t1 MTR_4g111640 24.859 177 117 4 4 173 12 179 1.47e-12 63.2
MS.gene21587.t1 MTR_2g083250 34.259 108 64 3 20 120 23 130 3.32e-12 63.2
MS.gene21587.t1 MTR_6g013170 30.986 142 89 5 4 137 7 147 6.60e-12 61.2
MS.gene21587.t1 MTR_7g086280 28.448 116 82 1 4 118 7 122 9.06e-12 60.8
MS.gene21587.t1 MTR_7g086140 30.000 120 82 2 4 121 7 126 1.16e-11 60.1
MS.gene21587.t1 MTR_7g086190 30.000 120 82 2 4 121 7 126 1.16e-11 60.1
MS.gene21587.t1 MTR_5g088000 27.737 137 92 4 4 137 2 134 2.48e-11 58.9
MS.gene21587.t1 MTR_3g089005 30.508 118 77 4 1 116 10 124 2.75e-11 58.5
MS.gene21587.t1 MTR_8g095013 31.356 118 76 3 4 117 7 123 3.92e-11 60.5
MS.gene21587.t1 MTR_8g088830 36.364 99 58 4 20 116 25 120 4.36e-11 57.8
MS.gene21587.t1 MTR_7g086230 26.724 116 84 1 4 118 7 122 7.33e-11 58.2
MS.gene21587.t1 MTR_4g007250 30.693 101 68 2 19 118 25 124 7.66e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21587.t1 AT5G07475 49.686 159 76 3 1 158 7 162 1.26e-48 156
MS.gene21587.t1 AT2G32300 43.590 117 65 1 2 117 5 121 1.60e-30 112
MS.gene21587.t1 AT2G32300 43.590 117 65 1 2 117 35 151 5.12e-30 111
MS.gene21587.t1 AT2G31050 39.683 126 72 3 7 130 13 136 6.34e-24 93.2
MS.gene21587.t1 AT5G26330 39.604 101 58 2 20 117 21 121 3.10e-21 85.9
MS.gene21587.t1 AT2G25060 39.073 151 70 5 4 136 16 162 3.36e-21 85.9
MS.gene21587.t1 AT3G60270 38.776 98 60 0 20 117 23 120 3.96e-21 85.9
MS.gene21587.t1 AT3G27200 35.833 120 73 3 1 117 3 121 4.57e-21 85.1
MS.gene21587.t1 AT2G26720 36.207 116 69 3 4 117 13 125 9.49e-21 85.1
MS.gene21587.t1 AT5G25090 38.596 114 65 2 9 117 13 126 3.21e-20 83.6
MS.gene21587.t1 AT4G31840 34.752 141 82 5 5 135 6 146 9.76e-18 76.6
MS.gene21587.t1 AT3G60280 37.755 98 61 0 20 117 21 118 1.02e-17 77.8
MS.gene21587.t1 AT1G72230 30.894 123 83 1 31 151 31 153 1.80e-17 76.3
MS.gene21587.t1 AT3G20570 40.000 105 59 2 20 120 27 131 2.24e-17 76.3
MS.gene21587.t1 AT3G17675 30.769 117 78 2 4 117 11 127 8.54e-17 72.8
MS.gene21587.t1 AT5G53870 33.333 120 69 3 5 113 4 123 2.07e-16 75.9
MS.gene21587.t1 AT5G53870 33.333 120 69 3 5 113 4 123 2.24e-16 75.9
MS.gene21587.t1 AT2G44790 38.000 100 56 2 20 117 29 124 1.43e-15 71.6
MS.gene21587.t1 AT4G28365 29.221 154 100 3 5 149 11 164 1.57e-15 71.2
MS.gene21587.t1 AT2G23990 36.029 136 74 5 9 131 13 148 2.43e-15 70.9
MS.gene21587.t1 AT1G45063 33.333 126 76 4 1 120 6 129 4.10e-15 71.2
MS.gene21587.t1 AT1G45063 33.333 120 72 5 16 130 142 258 1.59e-11 61.6
MS.gene21587.t1 AT1G45063 33.333 126 76 4 1 120 6 129 5.88e-15 71.6
MS.gene21587.t1 AT1G45063 33.333 120 72 5 16 130 142 258 2.06e-11 61.6
MS.gene21587.t1 AT1G79800 34.454 119 66 4 23 130 34 151 6.96e-15 69.3
MS.gene21587.t1 AT4G27520 35.897 117 68 3 4 117 16 128 2.18e-14 70.1
MS.gene21587.t1 AT4G12880 32.479 117 75 3 5 118 11 126 3.28e-14 66.6
MS.gene21587.t1 AT1G64640 36.364 121 70 3 19 132 29 149 3.36e-14 67.8
MS.gene21587.t1 AT3G53330 32.787 122 77 4 23 139 188 309 1.54e-13 67.4
MS.gene21587.t1 AT3G18590 30.894 123 77 3 2 117 5 126 2.42e-13 65.5
MS.gene21587.t1 AT4G30590 34.188 117 70 4 4 113 8 124 6.09e-13 64.3
MS.gene21587.t1 AT1G22480 32.231 121 76 2 1 120 1 116 8.13e-13 63.5
MS.gene21587.t1 AT5G57920 34.783 92 53 2 37 127 42 127 1.63e-12 62.8
MS.gene21587.t1 AT4G32490 33.058 121 76 3 4 121 14 132 1.78e-12 63.5
MS.gene21587.t1 AT5G57920 34.783 92 53 2 37 127 41 126 3.21e-12 62.0
MS.gene21587.t1 AT5G57920 32.710 107 61 3 26 127 59 159 3.43e-12 62.8
MS.gene21587.t1 AT3G01070 38.938 113 66 2 9 118 13 125 5.00e-12 61.2

Find 27 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGCGCTCTTCTTTGTTGA+TGG 0.292064 1.3:+63076334 None:intergenic
TCTGATCCAGCTACTGCTTT+TGG 0.293698 1.3:+63076370 None:intergenic
TATAGTTTATATTGCTGTTA+TGG 0.320304 1.3:-63076281 MS.gene21587:CDS
ACAAAAGTGTTGGCAAATTA+CGG 0.407043 1.3:-63076532 MS.gene21587:CDS
GTGTTGGCAAATTACGGAAA+TGG 0.435345 1.3:-63076526 MS.gene21587:CDS
CATTGACAAGAGCCGGAGAC+AGG 0.497365 1.3:-63076489 MS.gene21587:CDS
CTGTCTCCGGCTCTTGTCAA+TGG 0.513474 1.3:+63076490 None:intergenic
CTAGCACCAAAAGCAGTAGC+TGG 0.518482 1.3:-63076376 MS.gene21587:CDS
CTGTTGGAGATAGCTCTGGC+TGG 0.524284 1.3:-63077038 MS.gene21587:CDS
TACACTGTTGGAGATAGCTC+TGG 0.540204 1.3:-63077042 MS.gene21587:CDS
AACAAACTGTATTGCCTTGG+TGG 0.541279 1.3:-63076451 MS.gene21587:CDS
ACATACAAAATACCTGTCTC+CGG 0.555623 1.3:+63076477 None:intergenic
ACGGTGCCATTGACAAGAGC+CGG 0.574751 1.3:-63076496 MS.gene21587:CDS
ATGCAACACAACAAAAGTGT+TGG 0.577530 1.3:-63076542 MS.gene21587:CDS
TCAGCAACTACCTACACTGT+TGG 0.586019 1.3:-63077054 MS.gene21587:CDS
TGTTGGAGATAGCTCTGGCT+GGG 0.608785 1.3:-63077037 MS.gene21587:CDS
CAGTAGCTGGATCAGATCAA+AGG 0.609991 1.3:-63076363 MS.gene21587:CDS
AGAGCTATCTCCAACAGTGT+AGG 0.611707 1.3:+63077044 None:intergenic
CATGTTCATGTAGAAGATGA+CGG 0.616290 1.3:-63076418 MS.gene21587:CDS
ATCGACATACAGTGTGGACG+AGG 0.624451 1.3:-63076587 MS.gene21587:CDS
TTAGCTCTAATCTAGAAACA+TGG 0.627015 1.3:-63077011 MS.gene21587:CDS
TAGCTCTAATCTAGAAACAT+GGG 0.636260 1.3:-63077010 MS.gene21587:CDS
GGAAACAAACTGTATTGCCT+TGG 0.642701 1.3:-63076454 MS.gene21587:CDS
ACAAACTGTATTGCCTTGGT+GGG 0.646382 1.3:-63076450 MS.gene21587:CDS
CATGAAGTTTCATCCCACCA+AGG 0.728162 1.3:+63076437 None:intergenic
ATATTCATCGACATACAGTG+TGG 0.739211 1.3:-63076593 MS.gene21587:CDS
TTACGGAAATGGAAACACGA+CGG 0.772125 1.3:-63076515 MS.gene21587:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATATTTGTATTTTTGA+GGG - chr1.3:63076586-63076605 MS.gene21587:CDS 10.0%
!!! TATTTATATTTGTATTTTTG+AGG - chr1.3:63076585-63076604 MS.gene21587:CDS 10.0%
!! AATTATTGAATGAAAGATAA+AGG + chr1.3:63076419-63076438 None:intergenic 15.0%
!! ATAATTCAATTTATGTGAAT+TGG + chr1.3:63076494-63076513 None:intergenic 15.0%
!!! TGTTTTTATTTATCCATATA+TGG + chr1.3:63076556-63076575 None:intergenic 15.0%
!! ATGAATATTGAAAAACTGAA+AGG + chr1.3:63076755-63076774 None:intergenic 20.0%
!!! AACAGATTTTATGCATTTAT+AGG - chr1.3:63076688-63076707 MS.gene21587:intron 20.0%
!!! TATAGTTTATATTGCTGTTA+TGG - chr1.3:63077079-63077098 MS.gene21587:CDS 20.0%
!!! TGATTGATTTTGATATGTTA+TGG + chr1.3:63076521-63076540 None:intergenic 20.0%
! GCTGATAAAAATTTCAAGAT+AGG - chr1.3:63076375-63076394 MS.gene21587:CDS 25.0%
! TAACAAAAAAAGTTCCCAAA+TGG + chr1.3:63076727-63076746 None:intergenic 25.0%
!! GACAATTTTTACAACCATTT+GGG - chr1.3:63076710-63076729 MS.gene21587:intron 25.0%
ATTCTTCTTGTGACCATATA+TGG - chr1.3:63076540-63076559 MS.gene21587:CDS 30.0%
! CTGTTATGGTAGCTTTTTAT+GGG - chr1.3:63077093-63077112 MS.gene21587:CDS 30.0%
! GGACAATTTTTACAACCATT+TGG - chr1.3:63076709-63076728 MS.gene21587:intron 30.0%
! TCTTTCTCTTCTGTTTTCTT+TGG - chr1.3:63076269-63076288 MS.gene21587:CDS 30.0%
!! ACAAAAGTGTTGGCAAATTA+CGG - chr1.3:63076828-63076847 MS.gene21587:intron 30.0%
!! TAGCTCTAATCTAGAAACAT+GGG - chr1.3:63076350-63076369 MS.gene21587:CDS 30.0%
!! TTAGCTCTAATCTAGAAACA+TGG - chr1.3:63076349-63076368 MS.gene21587:CDS 30.0%
ACATACAAAATACCTGTCTC+CGG + chr1.3:63076886-63076905 None:intergenic 35.0%
ATATTCATCGACATACAGTG+TGG - chr1.3:63076767-63076786 MS.gene21587:intron 35.0%
CATGTTCATGTAGAAGATGA+CGG - chr1.3:63076942-63076961 MS.gene21587:intron 35.0%
! ATGCAACACAACAAAAGTGT+TGG - chr1.3:63076818-63076837 MS.gene21587:intron 35.0%
! GCTGTTATGGTAGCTTTTTA+TGG - chr1.3:63077092-63077111 MS.gene21587:CDS 35.0%
!! AAGAGCGCAGAATTTTCAAA+GGG - chr1.3:63077038-63077057 MS.gene21587:CDS 35.0%
AACAAACTGTATTGCCTTGG+TGG - chr1.3:63076909-63076928 MS.gene21587:intron 40.0%
ACAAACTGTATTGCCTTGGT+GGG - chr1.3:63076910-63076929 MS.gene21587:intron 40.0%
GGAAACAAACTGTATTGCCT+TGG - chr1.3:63076906-63076925 MS.gene21587:intron 40.0%
TTACGGAAATGGAAACACGA+CGG - chr1.3:63076845-63076864 MS.gene21587:intron 40.0%
! GGAGACAGGTATTTTGTATG+TGG - chr1.3:63076885-63076904 MS.gene21587:intron 40.0%
! GTGTTGGCAAATTACGGAAA+TGG - chr1.3:63076834-63076853 MS.gene21587:intron 40.0%
!! GAAGAGCGCAGAATTTTCAA+AGG - chr1.3:63077037-63077056 MS.gene21587:CDS 40.0%
!!! ACTGCTTTTGGTGCTAGATT+TGG + chr1.3:63076981-63077000 None:intergenic 40.0%
AGAGCTATCTCCAACAGTGT+AGG + chr1.3:63076319-63076338 None:intergenic 45.0%
CAGTAGCTGGATCAGATCAA+AGG - chr1.3:63076997-63077016 MS.gene21587:CDS 45.0%
CATGAAGTTTCATCCCACCA+AGG + chr1.3:63076926-63076945 None:intergenic 45.0%
TACACTGTTGGAGATAGCTC+TGG - chr1.3:63076318-63076337 MS.gene21587:CDS 45.0%
TCAGCAACTACCTACACTGT+TGG - chr1.3:63076306-63076325 MS.gene21587:CDS 45.0%
TCTGCGCTCTTCTTTGTTGA+TGG + chr1.3:63077029-63077048 None:intergenic 45.0%
! TCTGATCCAGCTACTGCTTT+TGG + chr1.3:63076993-63077012 None:intergenic 45.0%
ATCGACATACAGTGTGGACG+AGG - chr1.3:63076773-63076792 MS.gene21587:intron 50.0%
CTAGCACCAAAAGCAGTAGC+TGG - chr1.3:63076984-63077003 MS.gene21587:CDS 50.0%
!! TGTTGGAGATAGCTCTGGCT+GGG - chr1.3:63076323-63076342 MS.gene21587:CDS 50.0%
CATTGACAAGAGCCGGAGAC+AGG - chr1.3:63076871-63076890 MS.gene21587:intron 55.0%
CTGTCTCCGGCTCTTGTCAA+TGG + chr1.3:63076873-63076892 None:intergenic 55.0%
!! ACGGTGCCATTGACAAGAGC+CGG - chr1.3:63076864-63076883 MS.gene21587:intron 55.0%
!! CTGTTGGAGATAGCTCTGGC+TGG - chr1.3:63076322-63076341 MS.gene21587:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 63076252 63077130 63076252 ID=MS.gene21587
chr1.3 mRNA 63076252 63077130 63076252 ID=MS.gene21587.t1;Parent=MS.gene21587
chr1.3 exon 63076974 63077130 63076974 ID=MS.gene21587.t1.exon1;Parent=MS.gene21587.t1
chr1.3 CDS 63076974 63077130 63076974 ID=cds.MS.gene21587.t1;Parent=MS.gene21587.t1
chr1.3 exon 63076252 63076622 63076252 ID=MS.gene21587.t1.exon2;Parent=MS.gene21587.t1
chr1.3 CDS 63076252 63076622 63076252 ID=cds.MS.gene21587.t1;Parent=MS.gene21587.t1
Gene Sequence

>MS.gene21587

ATGGAAAAGTTGCTTCTTCTTTCTCTTCTGTTTTCTTTGGCAACTATCACATGTTCAGCAACTACCTACACTGTTGGAGATAGCTCTGGCTGGGACATTAGCTCTAATCTAGAAACATGGGTTGCTGATAAAAATTTCAAGATAGGTGATGCTCTTCGTAAGTACCTTTATCTTTCATTCAATAATTCATTTTCTATAAGCTATTGAAATCATACATATACTTACATGTTCTTCAAGTTCCAATTCACATAAATTGAATTATTTTTCCATAACATATCAAAATCAATCATTCTTCTTGTGACCATATATGGATAAATAAAAACATATTTTATGTATTTATATTTGTATTTTTGAGGGTTAAAATTCAATAGATAATGAAACTTGAAATGTCAAGTTGATTATCGTTTATAGCTTTTGATAACAAGATTTATGATAGAACAGATTTTATGCATTTATAGGACAATTTTTACAACCATTTGGGAACTTTTTTTGTTATTTTTCCTTTCAGTTTTTCAATATTCATCGACATACAGTGTGGACGAGGTTACAAAAGAAAACTTCGACACATGCAACACAACAAAAGTGTTGGCAAATTACGGAAATGGAAACACGACGGTGCCATTGACAAGAGCCGGAGACAGGTATTTTGTATGTGGAAACAAACTGTATTGCCTTGGTGGGATGAAACTTCATGTTCATGTAGAAGATGACGGCAAATCAATTTCGCCAAATCTAGCACCAAAAGCAGTAGCTGGATCAGATCAAAGGACTTCTCCATCAACAAAGAAGAGCGCAGAATTTTCAAAGGGAGTTGCAAATTGTGCACATATAGTTTATATTGCTGTTATGGTAGCTTTTTATGGGATGCTACAGATTTGA

Protein sequence

>MS.gene21587.t1

MEKLLLLSLLFSLATITCSATTYTVGDSSGWDISSNLETWVADKNFKIGDALLFQYSSTYSVDEVTKENFDTCNTTKVLANYGNGNTTVPLTRAGDRYFVCGNKLYCLGGMKLHVHVEDDGKSISPNLAPKAVAGSDQRTSPSTKKSAEFSKGVANCAHIVYIAVMVAFYGMLQI