AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21721

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21721.t1 MTR_5g008240 95.699 186 8 0 1 186 67 252 4.82e-133 372
MS.gene21721.t1 MTR_5g008240 95.699 186 8 0 1 186 43 228 4.30e-132 369
MS.gene21721.t1 MTR_5g008240 95.699 186 8 0 1 186 1 186 1.38e-131 366
MS.gene21721.t1 MTR_5g008240 96.947 131 4 0 56 186 38 168 2.15e-91 264
MS.gene21721.t1 MTR_4g086320 39.231 130 64 3 32 161 42 156 5.10e-26 98.2
MS.gene21721.t1 MTR_4g086330 41.667 132 59 5 33 161 53 169 1.21e-25 98.2
MS.gene21721.t1 MTR_0172s0050 33.742 163 89 7 41 186 63 223 3.43e-22 90.1
MS.gene21721.t1 MTR_7g044840 32.716 162 95 5 37 185 56 216 3.85e-22 90.1
MS.gene21721.t1 MTR_3g408350 33.974 156 87 5 41 182 59 212 4.29e-21 87.0
MS.gene21721.t1 MTR_6g084940 33.333 147 78 4 38 180 15 145 7.41e-21 84.3
MS.gene21721.t1 MTR_8g104890 40.152 132 64 4 32 163 25 141 6.49e-19 79.7
MS.gene21721.t1 MTR_8g104870 38.333 120 59 4 40 159 51 155 1.48e-17 76.6
MS.gene21721.t1 MTR_5g017650 38.525 122 57 6 40 158 51 157 4.90e-16 72.8
MS.gene21721.t1 MTR_3g408350 33.094 139 77 5 58 182 31 167 3.41e-15 70.5
MS.gene21721.t1 MTR_8g104830 32.432 148 85 4 36 182 4 137 4.24e-15 69.3
MS.gene21721.t1 MTR_2g101660 30.000 150 83 4 18 161 5 138 1.81e-14 68.2
MS.gene21721.t1 MTR_7g100870 29.143 175 79 6 41 180 63 227 1.54e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21721.t1 AT2G40935 64.516 186 61 2 2 182 3 188 9.70e-87 253
MS.gene21721.t1 AT2G40935 64.516 186 61 2 2 182 3 188 9.70e-87 253
MS.gene21721.t1 AT2G40935 64.516 186 61 2 2 182 3 188 9.70e-87 253
MS.gene21721.t1 AT2G40935 64.516 186 61 2 2 182 3 188 9.70e-87 253
MS.gene21721.t1 AT2G40935 64.516 186 61 2 2 182 3 188 9.70e-87 253
MS.gene21721.t1 AT2G40935 58.602 186 61 3 2 182 3 177 2.00e-74 221
MS.gene21721.t1 AT2G40935 70.079 127 38 0 56 182 38 164 1.40e-63 194
MS.gene21721.t1 AT2G40935 70.079 127 38 0 56 182 38 164 1.40e-63 194
MS.gene21721.t1 AT2G40935 70.079 127 38 0 56 182 38 164 1.40e-63 194
MS.gene21721.t1 AT3G18460 33.108 148 78 4 38 180 51 182 3.23e-23 91.7
MS.gene21721.t1 AT5G35525 39.098 133 66 3 29 161 4 121 4.78e-23 90.1
MS.gene21721.t1 AT3G18470 40.800 125 57 5 38 161 2 110 1.79e-22 88.2
MS.gene21721.t1 AT1G14870 35.252 139 72 4 23 161 1 121 6.86e-22 87.0
MS.gene21721.t1 AT1G14870 34.014 147 79 4 23 169 1 129 7.19e-22 86.7
MS.gene21721.t1 AT1G49030 35.294 153 81 4 9 161 59 193 9.82e-22 88.6
MS.gene21721.t1 AT1G14880 32.911 158 76 5 33 178 7 146 1.61e-20 83.6
MS.gene21721.t1 AT5G51400 33.125 160 91 5 41 186 62 219 4.00e-20 84.7
MS.gene21721.t1 AT2G45010 30.769 182 103 6 21 186 45 219 1.24e-19 83.6
MS.gene21721.t1 AT2G45010 30.723 166 95 5 37 186 12 173 1.25e-19 82.4
MS.gene21721.t1 AT1G68630 35.772 123 59 3 41 161 29 133 4.53e-18 77.4
MS.gene21721.t1 AT3G18450 29.814 161 84 5 1 161 25 156 9.49e-18 77.0
MS.gene21721.t1 AT2G37110 31.414 191 84 7 27 180 59 239 1.17e-17 78.2
MS.gene21721.t1 AT1G58320 37.903 124 58 5 40 161 16 122 5.23e-16 71.6
MS.gene21721.t1 AT1G68610 38.333 120 61 3 38 157 16 122 8.77e-16 71.6
MS.gene21721.t1 AT1G52200 31.707 123 65 2 41 161 53 158 1.20e-13 66.2
MS.gene21721.t1 AT1G52200 31.707 123 65 2 41 161 53 158 1.40e-13 66.2

Find 51 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGAAGAATGCAGAATTTC+TGG 0.136909 5.2:+2306780 MS.gene21721:CDS
CTTTGTCCTTGCTACCTCTT+TGG 0.163797 5.2:+2306760 MS.gene21721:CDS
CAAGACTCCACAATATAAAA+TGG 0.183305 5.2:-2306834 None:intergenic
GGATCAAATCAATGGTCTTC+TGG 0.256294 5.2:+2305690 MS.gene21721:CDS
CTGGGTTCTGGCACGTTCAT+GGG 0.276161 5.2:+2306799 MS.gene21721:CDS
CAAATTATACTTTGATCTTA+AGG 0.284771 5.2:-2306947 None:intergenic
TCTGGGTTCTGGCACGTTCA+TGG 0.317800 5.2:+2306798 MS.gene21721:CDS
AATGCAGAATTTCTGGGTTC+TGG 0.323080 5.2:+2306787 MS.gene21721:CDS
TTTGTCCTTGCTACCTCTTT+GGG 0.333564 5.2:+2306761 MS.gene21721:CDS
GGCTTCTGTGCAGGTTGTAT+AGG 0.363558 5.2:+2306733 MS.gene21721:intron
CAGCTCTTTCGCGTATTTCA+CGG 0.371837 5.2:-2307915 None:intergenic
AAGATCAAAGTATAATTTGC+CGG 0.391461 5.2:+2306951 MS.gene21721:CDS
TGGAATCTGACTGCATTGTC+TGG 0.397352 5.2:-2307990 None:intergenic
GGGAAGAATGCAGAATTTCT+GGG 0.403517 5.2:+2306781 MS.gene21721:CDS
GTGAAATACGCGAAAGAGCT+GGG 0.429175 5.2:+2307917 MS.gene21721:CDS
TCCACCTGCTTACATCCCAT+TGG 0.441939 5.2:+2305605 MS.gene21721:CDS
CCACCTGCTTACATCCCATT+GGG 0.443393 5.2:+2305606 MS.gene21721:CDS
CGTGAAATACGCGAAAGAGC+TGG 0.454935 5.2:+2307916 MS.gene21721:CDS
GACTGCATTGTCTGGACGGA+TGG 0.475373 5.2:-2307982 None:intergenic
TTCACGGTACTCTTGACAAA+TGG 0.476167 5.2:-2307899 None:intergenic
AAGACTCCACAATATAAAAT+GGG 0.490249 5.2:-2306833 None:intergenic
ATCTGACTGCATTGTCTGGA+CGG 0.518691 5.2:-2307986 None:intergenic
TCTGAATCTGATTGACCCAA+TGG 0.522303 5.2:-2305621 None:intergenic
ATGGTTGTGGCATTACTGAT+TGG 0.524056 5.2:-2308010 None:intergenic
ATTCTGCATTCTTCCCAAAG+AGG 0.532916 5.2:-2306774 None:intergenic
GTAGCAATGGATCAAATCAA+TGG 0.534849 5.2:+2305682 MS.gene21721:CDS
GCCTGCTGCTTATTAACCGA+TGG 0.548925 5.2:+2306865 MS.gene21721:CDS
CACAAGCATAACACGAAACA+AGG 0.556012 5.2:-2306912 None:intergenic
TGAAGCTACAGATATGAAGC+TGG 0.570972 5.2:+2307945 MS.gene21721:CDS
GCCATCGGTTAATAAGCAGC+AGG 0.572611 5.2:-2306866 None:intergenic
TGACCCAATGGGATGTAAGC+AGG 0.573937 5.2:-2305609 None:intergenic
TTCTTCCCAAAGAGGTAGCA+AGG 0.573956 5.2:-2306766 None:intergenic
TGGGTTCTGGCACGTTCATG+GGG 0.574682 5.2:+2306800 MS.gene21721:CDS
CTACTACCACCATGGTGTAG+AGG 0.574859 5.2:-2305660 None:intergenic
TAACACGAAACAAGGCATCC+AGG 0.577372 5.2:-2306904 None:intergenic
CTTGTTTCGTGTTATGCTTG+TGG 0.581137 5.2:+2306913 MS.gene21721:CDS
CATGGTGGTAGTAGTAGCAA+TGG 0.585897 5.2:+2305669 MS.gene21721:CDS
CTGAATCTGATTGACCCAAT+GGG 0.585943 5.2:-2305620 None:intergenic
TCCATTATTATGAAGAACCA+AGG 0.591583 5.2:+2305573 None:intergenic
TTATGAAGGAAGCACCGTGT+GGG 0.610712 5.2:+2307842 MS.gene21721:intron
ACTTTGATCTTAAGGTATTG+CGG 0.618835 5.2:-2306939 None:intergenic
TATGAAGGAAGCACCGTGTG+GGG 0.626423 5.2:+2307843 MS.gene21721:intron
TGAAATACGCGAAAGAGCTG+GGG 0.629440 5.2:+2307918 MS.gene21721:CDS
CCCAATGGGATGTAAGCAGG+TGG 0.640544 5.2:-2305606 None:intergenic
CAGGTGGAACATAACCACCT+TGG 0.653627 5.2:-2305590 None:intergenic
CATTGCTACTACTACCACCA+TGG 0.661910 5.2:-2305668 None:intergenic
CTTATGAAGGAAGCACCGTG+TGG 0.662327 5.2:+2307841 MS.gene21721:intron
GTCTCTCCTCTACACCATGG+TGG 0.678731 5.2:+2305654 MS.gene21721:CDS
ATTATTATGAAGAACCAAGG+TGG 0.688442 5.2:+2305576 None:intergenic
AGTGTCTCTCCTCTACACCA+TGG 0.703188 5.2:+2305651 MS.gene21721:CDS
TGGGTTACAAAATCCCCACA+CGG 0.723662 5.2:-2307856 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTGATGTATATATAAA+GGG + chr5.2:2306410-2306429 MS.gene21721:intron 10.0%
!! AAAAATCATTTGACTAATTA+CGG - chr5.2:2306576-2306595 None:intergenic 15.0%
!! AGTAAATTTGAAGAATAATT+AGG + chr5.2:2307178-2307197 MS.gene21721:intron 15.0%
!! GTTATTTGATGTATATATAA+AGG + chr5.2:2306409-2306428 MS.gene21721:intron 15.0%
!! TTAATAATGAAAAGAAAATC+TGG + chr5.2:2307782-2307801 MS.gene21721:intron 15.0%
!! AAAAAAAAAAAAAAAGGGCA+TGG + chr5.2:2306521-2306540 MS.gene21721:intron 20.0%
!! ACATCCAAAAAATTTAGATT+GGG - chr5.2:2307748-2307767 None:intergenic 20.0%
!! AGAAATTGAAATAAACTTGT+AGG + chr5.2:2307501-2307520 MS.gene21721:intron 20.0%
!! ATAAAATTTGATGTAAAGCA+TGG - chr5.2:2306991-2307010 None:intergenic 20.0%
!! ATGTGATAAAAATGAAAGAA+AGG + chr5.2:2307356-2307375 MS.gene21721:intron 20.0%
!! CAAATTATACTTTGATCTTA+AGG - chr5.2:2306950-2306969 None:intergenic 20.0%
!! CACCCAAAAAAAAAAAAAAA+GGG + chr5.2:2306516-2306535 MS.gene21721:intron 20.0%
!! TAATAGTAAAAGAACTGTTT+AGG + chr5.2:2307208-2307227 MS.gene21721:intron 20.0%
!! TGCTAAAATTTCTCTATTAA+AGG - chr5.2:2307711-2307730 None:intergenic 20.0%
!! TTTGTATGTTTGTTTGTTTA+AGG + chr5.2:2305798-2305817 MS.gene21721:intron 20.0%
!!! AAAGATTGGTTTCAAATTTT+CGG + chr5.2:2306712-2306731 MS.gene21721:intron 20.0%
!!! AAGTTCTGAATTTTATTGTT+TGG + chr5.2:2306245-2306264 MS.gene21721:intron 20.0%
!!! AGAAATTTTAGCACTTTATA+TGG + chr5.2:2307718-2307737 MS.gene21721:intron 20.0%
!!! AGATGCATTTTTTGAAAAAA+AGG + chr5.2:2307680-2307699 MS.gene21721:intron 20.0%
!!! ATATTCTTTTTGTCTTATGA+AGG + chr5.2:2307828-2307847 MS.gene21721:intron 20.0%
!!! ATGCCCTTTTTTTTTTTTTT+TGG - chr5.2:2306522-2306541 None:intergenic 20.0%
!!! TGCCCTTTTTTTTTTTTTTT+GGG - chr5.2:2306521-2306540 None:intergenic 20.0%
! AAGACTCCACAATATAAAAT+GGG - chr5.2:2306836-2306855 None:intergenic 25.0%
! AATAACTACTGATGTGAAAT+AGG - chr5.2:2306395-2306414 None:intergenic 25.0%
! AATAGTAAATGGTTCTTAAC+TGG + chr5.2:2307142-2307161 MS.gene21721:intron 25.0%
! AATTGGGGTAAAATAGTAAA+TGG + chr5.2:2307131-2307150 MS.gene21721:intron 25.0%
! ATAACTACTGATGTGAAATA+GGG - chr5.2:2306394-2306413 None:intergenic 25.0%
! ATACAAACATAATCAACAAG+AGG - chr5.2:2305785-2305804 None:intergenic 25.0%
! ATACTCTCTTATGTAGAAAT+TGG - chr5.2:2307406-2307425 None:intergenic 25.0%
! CATGGTTGTAATATTATTAC+CGG - chr5.2:2306973-2306992 None:intergenic 25.0%
! CCACCCAAAAAAAAAAAAAA+AGG + chr5.2:2306515-2306534 MS.gene21721:intron 25.0%
! CTAAGAAAAATCTTCTACTA+AGG - chr5.2:2305964-2305983 None:intergenic 25.0%
! GAACAGAAAATAGAAAGAAA+AGG - chr5.2:2307028-2307047 None:intergenic 25.0%
! GACATCCAAAAAATTTAGAT+TGG - chr5.2:2307749-2307768 None:intergenic 25.0%
! TAACTCACTGAACTTATTTA+TGG + chr5.2:2306335-2306354 MS.gene21721:intron 25.0%
! TATTCTTCAAATTTACTGAC+TGG - chr5.2:2307175-2307194 None:intergenic 25.0%
! TGTTTAACAAACACAAGAAA+AGG - chr5.2:2306294-2306313 None:intergenic 25.0%
!! AAGATCAAAGTATAATTTGC+CGG + chr5.2:2306951-2306970 MS.gene21721:CDS 25.0%
!! ACTTTTTGTAACATACAGAA+AGG - chr5.2:2306658-2306677 None:intergenic 25.0%
!! TTTCTTTCTATTTTCTGTTC+TGG + chr5.2:2307028-2307047 MS.gene21721:intron 25.0%
!!! ATCACCCAATCTAAATTTTT+TGG + chr5.2:2307741-2307760 MS.gene21721:intron 25.0%
!!! CAATTTCTAGTTTTCTTCTA+GGG - chr5.2:2307489-2307508 None:intergenic 25.0%
!!! CCTTTTTTTTTTTTTTTGGG+TGG - chr5.2:2306518-2306537 None:intergenic 25.0%
!!! TCAATTTCTAGTTTTCTTCT+AGG - chr5.2:2307490-2307509 None:intergenic 25.0%
!!! TCTGTTTTTTTTCGATAAAC+TGG + chr5.2:2306682-2306701 MS.gene21721:intron 25.0%
!!! TTTTTTTTTTTTTTGGGTGG+TGG - chr5.2:2306515-2306534 None:intergenic 25.0%
AAACTGGCAAATGAAAAGAT+TGG + chr5.2:2306698-2306717 MS.gene21721:intron 30.0%
AACTGTTTAGGTATGTTAGT+AGG + chr5.2:2307220-2307239 MS.gene21721:intron 30.0%
ACACAGATGACAACAAAAAT+GGG - chr5.2:2307878-2307897 None:intergenic 30.0%
ACTGTTTAGGTATGTTAGTA+GGG + chr5.2:2307221-2307240 MS.gene21721:intron 30.0%
AGATAGATTGATAAGCTCAT+TGG + chr5.2:2306025-2306044 MS.gene21721:intron 30.0%
CAAGACTCCACAATATAAAA+TGG - chr5.2:2306837-2306856 None:intergenic 30.0%
CACAATGCATAATATATGCA+AGG + chr5.2:2306470-2306489 MS.gene21721:intron 30.0%
CATCCTAAAAATAGCATTCT+CGG - chr5.2:2306126-2306145 None:intergenic 30.0%
CTAGGAAAATATTTAGGCAT+AGG - chr5.2:2307656-2307675 None:intergenic 30.0%
CTGCAACTAGGAAAATATTT+AGG - chr5.2:2307662-2307681 None:intergenic 30.0%
TAACTACTGATGTGAAATAG+GGG - chr5.2:2306393-2306412 None:intergenic 30.0%
TCACTGAACTTATTTATGGT+TGG + chr5.2:2306339-2306358 MS.gene21721:intron 30.0%
TGATGTATATATAAAGGGCA+TGG + chr5.2:2306415-2306434 MS.gene21721:intron 30.0%
! CAGAATTTCTGAATCCTTTT+AGG + chr5.2:2307545-2307564 MS.gene21721:intron 30.0%
! GTGTTACCCATTTTATATTG+TGG + chr5.2:2306827-2306846 MS.gene21721:CDS 30.0%
! TGAGACTGGTTAATGTAATT+GGG + chr5.2:2307115-2307134 MS.gene21721:intron 30.0%
!! ACTTTGATCTTAAGGTATTG+CGG - chr5.2:2306942-2306961 None:intergenic 30.0%
AAAAGATCTCACTGATCAGT+GGG - chr5.2:2306175-2306194 None:intergenic 35.0%
AACTACTGATGTGAAATAGG+GGG - chr5.2:2306392-2306411 None:intergenic 35.0%
ACAGTACATGTTCCTTATTG+TGG - chr5.2:2306096-2306115 None:intergenic 35.0%
ACGAATGTAGGAGAAAAATG+AGG + chr5.2:2305919-2305938 MS.gene21721:intron 35.0%
AGATTGATAAGCTCATTGGT+TGG + chr5.2:2306029-2306048 MS.gene21721:intron 35.0%
AGGTAAGTTCACAAACTTTG+AGG + chr5.2:2307575-2307594 MS.gene21721:intron 35.0%
AGTAAATGGTTCTTAACTGG+AGG + chr5.2:2307145-2307164 MS.gene21721:intron 35.0%
ATTTCTCTTGAAAACACTCC+AGG + chr5.2:2307606-2307625 MS.gene21721:intron 35.0%
CAAAAAATGCATCTGCAACT+AGG - chr5.2:2307674-2307693 None:intergenic 35.0%
CACACAGATGACAACAAAAA+TGG - chr5.2:2307879-2307898 None:intergenic 35.0%
CAGTACATGTTCCTTATTGT+GGG - chr5.2:2306095-2306114 None:intergenic 35.0%
CATAATATATGCAAGGTCCA+GGG + chr5.2:2306477-2306496 MS.gene21721:intron 35.0%
CGAATGTAGGAGAAAAATGA+GGG + chr5.2:2305920-2305939 MS.gene21721:intron 35.0%
CTGATAGAGTTAGATAATGC+AGG + chr5.2:2307435-2307454 MS.gene21721:intron 35.0%
CTTACCTTGAACTACCTAAA+AGG - chr5.2:2307562-2307581 None:intergenic 35.0%
CTTGACAATAAGGATTAGGA+GGG + chr5.2:2306221-2306240 MS.gene21721:intron 35.0%
GAATGTAGGAGAAAAATGAG+GGG + chr5.2:2305921-2305940 MS.gene21721:intron 35.0%
GGTAAGTTCACAAACTTTGA+GGG + chr5.2:2307576-2307595 MS.gene21721:intron 35.0%
GTAGCAATGGATCAAATCAA+TGG + chr5.2:2305682-2305701 MS.gene21721:CDS 35.0%
TAAAAGATCTCACTGATCAG+TGG - chr5.2:2306176-2306195 None:intergenic 35.0%
TCTATAGGAAGTGAAAAGCT+GGG - chr5.2:2307262-2307281 None:intergenic 35.0%
TTGACAATAAGGATTAGGAG+GGG + chr5.2:2306222-2306241 MS.gene21721:intron 35.0%
! AGTTAGATAATGCAGGTTGA+AGG + chr5.2:2307442-2307461 MS.gene21721:intron 35.0%
! CTTACCTTGTTTAATCACCA+AGG + chr5.2:2305757-2305776 MS.gene21721:intron 35.0%
! CTTGGTGATTAAACAAGGTA+AGG - chr5.2:2305759-2305778 None:intergenic 35.0%
! GAGACTGGTTAATGTAATTG+GGG + chr5.2:2307116-2307135 MS.gene21721:intron 35.0%
! GTGAGACTGGTTAATGTAAT+TGG + chr5.2:2307114-2307133 MS.gene21721:intron 35.0%
! GTTAGATAATGCAGGTTGAA+GGG + chr5.2:2307443-2307462 MS.gene21721:intron 35.0%
!! AGTTTTCTTCTAGGGAAAAC+TGG - chr5.2:2307481-2307500 None:intergenic 35.0%
!! GAATCCTTTTAGGTAGTTCA+AGG + chr5.2:2307555-2307574 MS.gene21721:intron 35.0%
!! TTTTGACTGCCTTGACAATA+AGG + chr5.2:2306211-2306230 MS.gene21721:intron 35.0%
!!! ATTTTTAGGATGGATGACAC+TGG + chr5.2:2306134-2306153 MS.gene21721:intron 35.0%
!!! CGAGAATGCTATTTTTAGGA+TGG + chr5.2:2306124-2306143 MS.gene21721:intron 35.0%
AGAAAAATGAGGGGAACTAG+AGG + chr5.2:2305930-2305949 MS.gene21721:intron 40.0%
ATCCAGGTAAACCCAAAAAC+AGG - chr5.2:2306891-2306910 None:intergenic 40.0%
ATTCTGCATTCTTCCCAAAG+AGG - chr5.2:2306777-2306796 None:intergenic 40.0%
CACAAGCATAACACGAAACA+AGG - chr5.2:2306915-2306934 None:intergenic 40.0%
CCTAAAAATAGCATTCTCGG+CGG - chr5.2:2306123-2306142 None:intergenic 40.0%
CCTCCTAATCCTTATTGTCA+AGG - chr5.2:2306223-2306242 None:intergenic 40.0%
CCTTGACAATAAGGATTAGG+AGG + chr5.2:2306220-2306239 MS.gene21721:intron 40.0%
CTATAGGAAGTGAAAAGCTG+GGG - chr5.2:2307261-2307280 None:intergenic 40.0%
CTGAATCTGATTGACCCAAT+GGG - chr5.2:2305623-2305642 None:intergenic 40.0%
CTGCCTTGACAATAAGGATT+AGG + chr5.2:2306217-2306236 MS.gene21721:intron 40.0%
CTTGTTTCGTGTTATGCTTG+TGG + chr5.2:2306913-2306932 MS.gene21721:CDS 40.0%
GCATAATATATGCAAGGTCC+AGG + chr5.2:2306476-2306495 MS.gene21721:intron 40.0%
GGATCAAATCAATGGTCTTC+TGG + chr5.2:2305690-2305709 MS.gene21721:CDS 40.0%
GGGAAGAATGCAGAATTTCT+GGG + chr5.2:2306781-2306800 MS.gene21721:CDS 40.0%
GTCTATAGGAAGTGAAAAGC+TGG - chr5.2:2307263-2307282 None:intergenic 40.0%
GTTGGAACCTTATGTTGGAT+GGG + chr5.2:2306051-2306070 MS.gene21721:intron 40.0%
TAAACCCAAAAACAGGCCAT+CGG - chr5.2:2306884-2306903 None:intergenic 40.0%
TCTGAATCTGATTGACCCAA+TGG - chr5.2:2305624-2305643 None:intergenic 40.0%
TGAAGCTACAGATATGAAGC+TGG + chr5.2:2307945-2307964 MS.gene21721:CDS 40.0%
TGGGAAGAATGCAGAATTTC+TGG + chr5.2:2306780-2306799 MS.gene21721:CDS 40.0%
TGTTCCTTATTGTGGGTCTA+TGG - chr5.2:2306088-2306107 None:intergenic 40.0%
TTCACGGTACTCTTGACAAA+TGG - chr5.2:2307902-2307921 None:intergenic 40.0%
TTTGTCCTTGCTACCTCTTT+GGG + chr5.2:2306761-2306780 MS.gene21721:CDS 40.0%
! AATGCAGAATTTCTGGGTTC+TGG + chr5.2:2306787-2306806 MS.gene21721:CDS 40.0%
! ATTAACCGATGGCCTGTTTT+TGG + chr5.2:2306876-2306895 MS.gene21721:CDS 40.0%
! CAGTGAGATCTTTTACGAGT+AGG + chr5.2:2306181-2306200 MS.gene21721:intron 40.0%
! TGATAAGCTCATTGGTTGGT+TGG + chr5.2:2306033-2306052 MS.gene21721:intron 40.0%
!! ATAATCAACAAGAGGCACCT+TGG - chr5.2:2305777-2305796 None:intergenic 40.0%
!! TGTTGCATTTTGAGTGAGAC+TGG + chr5.2:2307101-2307120 MS.gene21721:intron 40.0%
!! TTAACCGATGGCCTGTTTTT+GGG + chr5.2:2306877-2306896 MS.gene21721:CDS 40.0%
ATCTCACTGATCAGTGGGAT+AGG - chr5.2:2306170-2306189 None:intergenic 45.0%
ATCTGACTGCATTGTCTGGA+CGG - chr5.2:2307989-2308008 None:intergenic 45.0%
CAGCTCTTTCGCGTATTTCA+CGG - chr5.2:2307918-2307937 None:intergenic 45.0%
CATGGTGGTAGTAGTAGCAA+TGG + chr5.2:2305669-2305688 MS.gene21721:CDS 45.0%
CATTGCTACTACTACCACCA+TGG - chr5.2:2305671-2305690 None:intergenic 45.0%
CTTATGTTGGATGGGAAGAC+CGG + chr5.2:2306059-2306078 MS.gene21721:intron 45.0%
CTTTGTCCTTGCTACCTCTT+TGG + chr5.2:2306760-2306779 MS.gene21721:CDS 45.0%
GCTACCATAGACCCACAATA+AGG + chr5.2:2306081-2306100 MS.gene21721:intron 45.0%
GGTTGGAACCTTATGTTGGA+TGG + chr5.2:2306050-2306069 MS.gene21721:intron 45.0%
GTGAAATACGCGAAAGAGCT+GGG + chr5.2:2307917-2307936 MS.gene21721:CDS 45.0%
GTGCTAGATGACACGAATGT+AGG + chr5.2:2305907-2305926 MS.gene21721:intron 45.0%
TATTGTGGGTCTATGGTAGC+CGG - chr5.2:2306081-2306100 None:intergenic 45.0%
TGAAATACGCGAAAGAGCTG+GGG + chr5.2:2307918-2307937 MS.gene21721:CDS 45.0%
TGGAATCTGACTGCATTGTC+TGG - chr5.2:2307993-2308012 None:intergenic 45.0%
TGGGTTACAAAATCCCCACA+CGG - chr5.2:2307859-2307878 None:intergenic 45.0%
TTCTTCCCAAAGAGGTAGCA+AGG - chr5.2:2306769-2306788 None:intergenic 45.0%
! CAAATTTTCGGCTTCTGTGC+AGG + chr5.2:2306724-2306743 MS.gene21721:intron 45.0%
! GGCACCTTGGTGATTAAACA+AGG - chr5.2:2305764-2305783 None:intergenic 45.0%
! GGTTGGTTGGAACCTTATGT+TGG + chr5.2:2306046-2306065 MS.gene21721:intron 45.0%
! TAACACGAAACAAGGCATCC+AGG - chr5.2:2306907-2306926 None:intergenic 45.0%
! TGAAAACACTCCAGGTACTG+TGG + chr5.2:2307614-2307633 MS.gene21721:intron 45.0%
!! TGCTGCTACTTGCTGTCTAT+AGG - chr5.2:2307277-2307296 None:intergenic 45.0%
!! TTATGAAGGAAGCACCGTGT+GGG + chr5.2:2307842-2307861 MS.gene21721:intron 45.0%
!!! CCGCCGAGAATGCTATTTTT+AGG + chr5.2:2306120-2306139 MS.gene21721:intron 45.0%
!!! TTTTTTTTGGGTGGTGGCCA+GGG - chr5.2:2306509-2306528 None:intergenic 45.0%
!!! TTTTTTTTTGGGTGGTGGCC+AGG - chr5.2:2306510-2306529 None:intergenic 45.0%
AGTGTCTCTCCTCTACACCA+TGG + chr5.2:2305651-2305670 MS.gene21721:CDS 50.0%
CAGGTGGAACATAACCACCT+TGG - chr5.2:2305593-2305612 None:intergenic 50.0%
CCACCTGCTTACATCCCATT+GGG + chr5.2:2305606-2305625 MS.gene21721:CDS 50.0%
CGGTCTTCCCATCCAACATA+AGG - chr5.2:2306061-2306080 None:intergenic 50.0%
CGTGAAATACGCGAAAGAGC+TGG + chr5.2:2307916-2307935 MS.gene21721:CDS 50.0%
CTACTACCACCATGGTGTAG+AGG - chr5.2:2305663-2305682 None:intergenic 50.0%
CTCATCTCAGATGATGTGCC+AGG - chr5.2:2307309-2307328 None:intergenic 50.0%
GGCTTCTGTGCAGGTTGTAT+AGG + chr5.2:2306733-2306752 MS.gene21721:intron 50.0%
GTAGCAGCAACTTTGAGTCC+TGG + chr5.2:2307288-2307307 MS.gene21721:intron 50.0%
TCCACCTGCTTACATCCCAT+TGG + chr5.2:2305605-2305624 MS.gene21721:CDS 50.0%
TGACCCAATGGGATGTAAGC+AGG - chr5.2:2305612-2305631 None:intergenic 50.0%
! GCCATCGGTTAATAAGCAGC+AGG - chr5.2:2306869-2306888 None:intergenic 50.0%
! GCCTGCTGCTTATTAACCGA+TGG + chr5.2:2306865-2306884 MS.gene21721:CDS 50.0%
!! CTTATGAAGGAAGCACCGTG+TGG + chr5.2:2307841-2307860 MS.gene21721:intron 50.0%
!! GAGAAGTGTTCCACAGTACC+TGG - chr5.2:2307627-2307646 None:intergenic 50.0%
!! TATGAAGGAAGCACCGTGTG+GGG + chr5.2:2307843-2307862 MS.gene21721:intron 50.0%
!!! GGCCTGTTTTTGGGTTTACC+TGG + chr5.2:2306886-2306905 MS.gene21721:CDS 50.0%
AAGGTCCAGGGTTCAAACCC+TGG + chr5.2:2306489-2306508 MS.gene21721:intron 55.0%
CCAACTGAGCTAAGCTCACG+AGG - chr5.2:2306446-2306465 None:intergenic 55.0%
CCCAATGGGATGTAAGCAGG+TGG - chr5.2:2305609-2305628 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG + chr5.2:2306443-2306462 MS.gene21721:intron 55.0%
GACTGCATTGTCTGGACGGA+TGG - chr5.2:2307985-2308004 None:intergenic 55.0%
GTCTCTCCTCTACACCATGG+TGG + chr5.2:2305654-2305673 MS.gene21721:CDS 55.0%
! CTGGGTTCTGGCACGTTCAT+GGG + chr5.2:2306799-2306818 MS.gene21721:CDS 55.0%
! TCTGGGTTCTGGCACGTTCA+TGG + chr5.2:2306798-2306817 MS.gene21721:CDS 55.0%
! TGGGTTCTGGCACGTTCATG+GGG + chr5.2:2306800-2306819 MS.gene21721:CDS 55.0%
GGTGGCCAGGGTTTGAACCC+TGG - chr5.2:2306497-2306516 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 2305582 2308020 2305582 ID=MS.gene21721
chr5.2 mRNA 2305582 2308020 2305582 ID=MS.gene21721.t1;Parent=MS.gene21721
chr5.2 exon 2305582 2305744 2305582 ID=MS.gene21721.t1.exon1;Parent=MS.gene21721.t1
chr5.2 CDS 2305582 2305744 2305582 ID=cds.MS.gene21721.t1;Parent=MS.gene21721.t1
chr5.2 exon 2306746 2306972 2306746 ID=MS.gene21721.t1.exon2;Parent=MS.gene21721.t1
chr5.2 CDS 2306746 2306972 2306746 ID=cds.MS.gene21721.t1;Parent=MS.gene21721.t1
chr5.2 exon 2307850 2308020 2307850 ID=MS.gene21721.t1.exon3;Parent=MS.gene21721.t1
chr5.2 CDS 2307850 2308020 2307850 ID=cds.MS.gene21721.t1;Parent=MS.gene21721.t1
Gene Sequence

>MS.gene21721

ATGAAGAACCAAGGTGGTTATGTTCCACCTGCTTACATCCCATTGGGTCAATCAGATTCAGAGACAATGAGTGTCTCTCCTCTACACCATGGTGGTAGTAGTAGCAATGGATCAAATCAATGGTCTTCTGGAATCTGTGCTTGTTTCGATGATATGCAGAGCTGTATGCTACTTCCTTACCTTGTTTAATCACCAAGGTGCCTCTTGTTGATTATGTTTGTATGTTTGTTTGTTTAAGGATATTTGTTTGTGTCGTTGTTGTTCTTTATATCATAATTTTGTTAAGTATTTACTAATTTTAAGAATATTAACTAGTATAGAGCTCGTGCTAGATGACACGAATGTAGGAGAAAAATGAGGGGAACTAGAGGTATGAATTCCTTAGTAGAAGATTTTTCTTAGCATAGATAGATAGAATCTAGAAGATAGATAGATAGATAGATAGATAGATTGATAAGCTCATTGGTTGGTTGGAACCTTATGTTGGATGGGAAGACCGGCTACCATAGACCCACAATAAGGAACATGTACTGTTACACCGCCGAGAATGCTATTTTTAGGATGGATGACACTGGAAAATGCATGCCTATCCCACTGATCAGTGAGATCTTTTACGAGTAGGATCAATATTTTGACTGCCTTGACAATAAGGATTAGGAGGGGAAGTTCTGAATTTTATTGTTTGGCACTGTCTTTTGATTGATTATAGCCTTTTCTTGTGTTTGTTAAACAATACTCAATTAAGTGAATTCTTAACTCACTGAACTTATTTATGGTTGGTACTAGACATTCTTTTATAAAACTTTTCCCCCTATTTCACATCAGTAGTTATTTGATGTATATATAAAGGGCATGGAGTGTCCTCGTGAGCTTAGCTCAGTTGGTATGCACAATGCATAATATATGCAAGGTCCAGGGTTCAAACCCTGGCCACCACCCAAAAAAAAAAAAAAAGGGCATGGAGTAAGATAAAAATCATCAATATTTGACACCGTAATTAGTCAAATGATTTTTTAGCTTGTTGATTAAAATATTTAAATTATAGTATAAAAAGCTCTTGTCTTTTGTTTTTACCTTTCTGTATGTTACAAAAAGTTTGTTCTGTTTTTTTTCGATAAACTGGCAAATGAAAAGATTGGTTTCAAATTTTCGGCTTCTGTGCAGGTTGTATAGGTTGTCTTTGTCCTTGCTACCTCTTTGGGAAGAATGCAGAATTTCTGGGTTCTGGCACGTTCATGGGGTCATGTGTTACCCATTTTATATTGTGGAGTCTTGTTAATGCAGCCTGCTGCTTATTAACCGATGGCCTGTTTTTGGGTTTACCTGGATGCCTTGTTTCGTGTTATGCTTGTGGCTACCGCAATACCTTAAGATCAAAGTATAATTTGCCGGTAATAATATTACAACCATGCTTTACATCAAATTTTATAACTTGTTTTTTCACCTTTTCTTTCTATTTTCTGTTCTGGCTGTTATGTTTCAAACTTTTAAATATAAGTGTTATATTTAGTTATTTCAATGTTGCATTTTGAGTGAGACTGGTTAATGTAATTGGGGTAAAATAGTAAATGGTTCTTAACTGGAGGTTGACCAGTCAGTAAATTTGAAGAATAATTAGGCACATTCTAATAGTAAAAGAACTGTTTAGGTATGTTAGTAGGGAACGAGCATAAATTCCCCAGCTTTTCACTTCCTATAGACAGCAAGTAGCAGCAACTTTGAGTCCTGGCACATCATCTGAGATGAGTGAATTTAAAATATGTCTAAAATTTAAATGTGATAAAAATGAAAGAAAGGAGATCTGTCGTTATATCTATATCACCAATTTCTACATAAGAGAGTATCTATGTCATCTGATAGAGTTAGATAATGCAGGTTGAAGGGTGCTATTCAAGTCCAGTTTTCCCTAGAAGAAAACTAGAAATTGAAATAAACTTGTAGGACTTGCTCTTTGTTCATATTTCAGAATTTCTGAATCCTTTTAGGTAGTTCAAGGTAAGTTCACAAACTTTGAGGGAATGCATATTTCTCTTGAAAACACTCCAGGTACTGTGGAACACTTCTCAACATGCCTATGCCTAAATATTTTCCTAGTTGCAGATGCATTTTTTGAAAAAAAGGATTTGCCTTTAATAGAGAAATTTTAGCACTTTATATGGATCACCCAATCTAAATTTTTTGGATGTCTCTTGTGTAAATATTAATAATGAAAAGAAAATCTGGTGATGCAAAGCATAATACTAATCATATTCTTTTTGTCTTATGAAGGAAGCACCGTGTGGGGATTTTGTAACCCATTTTTGTTGTCATCTGTGTGCCATTTGTCAAGAGTACCGTGAAATACGCGAAAGAGCTGGGGATTCTGAAGCTACAGATATGAAGCTGGCAGTAGTTACAGCTCCATCCGTCCAGACAATGCAGTCAGATTCCAATCAGTAA
Protein sequence

>MS.gene21721.t1

MKNQGGYVPPAYIPLGQSDSETMSVSPLHHGGSSSNGSNQWSSGICACFDDMQSCCIGCLCPCYLFGKNAEFLGSGTFMGSCVTHFILWSLVNAACCLLTDGLFLGLPGCLVSCYACGYRNTLRSKYNLPEAPCGDFVTHFCCHLCAICQEYREIRERAGDSEATDMKLAVVTAPSVQTMQSDSNQ