Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21756.t1 | XP_024631911.1 | 87.9 | 174 | 15 | 1 | 1 | 174 | 416 | 583 | 1.40E-57 | 232.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21756.t1 | A0A072TGZ8 | 90.3 | 124 | 12 | 0 | 51 | 174 | 7 | 130 | 6.3e-36 | 160.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057049 | MS.gene21756 | 0.82857 | 8.35E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21756.t1 | MTR_0125s0060 | 91.129 | 124 | 11 | 0 | 51 | 174 | 7 | 130 | 2.22e-76 | 224 |
| MS.gene21756.t1 | MTR_2g035110 | 77.612 | 134 | 30 | 0 | 46 | 179 | 2 | 135 | 4.19e-67 | 201 |
| MS.gene21756.t1 | MTR_8g066845 | 57.547 | 106 | 25 | 3 | 75 | 179 | 1 | 87 | 5.49e-27 | 98.2 |
| MS.gene21756.t1 | MTR_4g012560 | 69.355 | 62 | 14 | 1 | 92 | 153 | 24 | 80 | 2.51e-17 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCCATCTGGTTTCCCTTT+TGG | 0.170423 | 5.2:+1987328 | None:intergenic |
| AATTGACAATGATATTTATA+TGG | 0.247121 | 5.2:-1987125 | MS.gene21756:CDS |
| AACAAGAAGGGGCGCATATT+TGG | 0.259894 | 5.2:-1987085 | MS.gene21756:CDS |
| TGAATTGAAGTGTTCATATT+AGG | 0.299166 | 5.2:+1986860 | None:intergenic |
| TCATTGTCAATTTCTTCATT+AGG | 0.306295 | 5.2:+1987136 | None:intergenic |
| CCATTATCACCTTCTCCTTC+CGG | 0.338322 | 5.2:+1986836 | None:intergenic |
| AAGGGGCGCATATTTGGATT+AGG | 0.373630 | 5.2:-1987079 | MS.gene21756:CDS |
| GATATTTATATGGAAATTGT+TGG | 0.391812 | 5.2:-1987115 | MS.gene21756:CDS |
| GAGCGCTTGGAGCAAATGAT+TGG | 0.408396 | 5.2:-1986893 | MS.gene21756:CDS |
| GTGTTTCTCAAACGTGAGTT+TGG | 0.410171 | 5.2:+1987189 | None:intergenic |
| CCGGAAGGAGAAGGTGATAA+TGG | 0.416904 | 5.2:-1986836 | MS.gene21756:CDS |
| GATAAACTTCATAGACCAAA+AGG | 0.428714 | 5.2:-1987343 | MS.gene21756:CDS |
| GTTTCATTAAACCACCCATC+TGG | 0.467331 | 5.2:+1987316 | None:intergenic |
| TTAGAGAGTGGAATTGGATA+GGG | 0.471643 | 5.2:-1987389 | MS.gene21756:intron |
| TTTAGAGAGTGGAATTGGAT+AGG | 0.476342 | 5.2:-1987390 | MS.gene21756:intron |
| AGAAACGCTAAAACTGAAGA+TGG | 0.499466 | 5.2:-1986915 | MS.gene21756:CDS |
| AAAACTGAAGATGGAGCGCT+TGG | 0.500382 | 5.2:-1986906 | MS.gene21756:CDS |
| TGTTGGAGGCATTAACAAGA+AGG | 0.515610 | 5.2:-1987098 | MS.gene21756:CDS |
| ATTTATATGGAAATTGTTGG+AGG | 0.522832 | 5.2:-1987112 | MS.gene21756:CDS |
| AGCGCTTGGAGCAAATGATT+GGG | 0.526657 | 5.2:-1986892 | MS.gene21756:CDS |
| TATGAACACTTCAATTCAGC+CGG | 0.533653 | 5.2:-1986855 | MS.gene21756:CDS |
| TCAATTCAGCCGGAAGGAGA+AGG | 0.553755 | 5.2:-1986845 | MS.gene21756:CDS |
| GTTGGAGGCATTAACAAGAA+GGG | 0.555037 | 5.2:-1987097 | MS.gene21756:CDS |
| AGACCAAAAGGGAAACCAGA+TGG | 0.568337 | 5.2:-1987331 | MS.gene21756:CDS |
| GACCAAAAGGGAAACCAGAT+GGG | 0.568542 | 5.2:-1987330 | MS.gene21756:CDS |
| GCTACATCGGTAGAAATTGA+AGG | 0.569826 | 5.2:+1987016 | None:intergenic |
| ATAAACTTCATAGACCAAAA+GGG | 0.570045 | 5.2:-1987342 | MS.gene21756:CDS |
| TTGGAGGCATTAACAAGAAG+GGG | 0.580609 | 5.2:-1987096 | MS.gene21756:CDS |
| TCGTAAATCTCAAACCATGT+TGG | 0.588016 | 5.2:+1987364 | None:intergenic |
| TCGGTAGAAATTGAAGGCGA+TGG | 0.604104 | 5.2:+1987022 | None:intergenic |
| AACACTTCAATTCAGCCGGA+AGG | 0.621409 | 5.2:-1986851 | MS.gene21756:CDS |
| TTTGAGAAACACTCTCAAAG+TGG | 0.633906 | 5.2:-1987178 | MS.gene21756:CDS |
| AATTGGATAGGGAACCAACA+TGG | 0.651250 | 5.2:-1987378 | MS.gene21756:CDS |
| CAAAAGGGAAACCAGATGGG+TGG | 0.669221 | 5.2:-1987327 | MS.gene21756:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGACAATGATATTTATA+TGG | - | chr5.2:1987149-1987168 | MS.gene21756:CDS | 15.0% |
| !! | GATATTTATATGGAAATTGT+TGG | - | chr5.2:1987159-1987178 | MS.gene21756:CDS | 20.0% |
| ! | ATAAACTTCATAGACCAAAA+GGG | - | chr5.2:1986932-1986951 | MS.gene21756:CDS | 25.0% |
| ! | ATTTATATGGAAATTGTTGG+AGG | - | chr5.2:1987162-1987181 | MS.gene21756:CDS | 25.0% |
| ! | TCATTGTCAATTTCTTCATT+AGG | + | chr5.2:1987141-1987160 | None:intergenic | 25.0% |
| !! | TGAATTGAAGTGTTCATATT+AGG | + | chr5.2:1987417-1987436 | None:intergenic | 25.0% |
| GATAAACTTCATAGACCAAA+AGG | - | chr5.2:1986931-1986950 | MS.gene21756:CDS | 30.0% | |
| TTCGAAAAAAGTTGCTTCTA+TGG | - | chr5.2:1987284-1987303 | MS.gene21756:intron | 30.0% | |
| !!! | TATGTTTTAGAGAGTGGAAT+TGG | - | chr5.2:1986879-1986898 | MS.gene21756:CDS | 30.0% |
| !!! | TTTTTTCGAAGAAGCTACAT+CGG | + | chr5.2:1987274-1987293 | None:intergenic | 30.0% |
| AGAAACGCTAAAACTGAAGA+TGG | - | chr5.2:1987359-1987378 | MS.gene21756:CDS | 35.0% | |
| GAAAAAAGTTGCTTCTATGG+AGG | - | chr5.2:1987287-1987306 | MS.gene21756:intron | 35.0% | |
| TATGAACACTTCAATTCAGC+CGG | - | chr5.2:1987419-1987438 | MS.gene21756:intron | 35.0% | |
| TCGTAAATCTCAAACCATGT+TGG | + | chr5.2:1986913-1986932 | None:intergenic | 35.0% | |
| TTAGAGAGTGGAATTGGATA+GGG | - | chr5.2:1986885-1986904 | MS.gene21756:CDS | 35.0% | |
| TTTAGAGAGTGGAATTGGAT+AGG | - | chr5.2:1986884-1986903 | MS.gene21756:CDS | 35.0% | |
| TTTGAGAAACACTCTCAAAG+TGG | - | chr5.2:1987096-1987115 | MS.gene21756:CDS | 35.0% | |
| !! | CATACTAACTTGTTGTTTGC+TGG | - | chr5.2:1986855-1986874 | MS.gene21756:CDS | 35.0% |
| AATTGGATAGGGAACCAACA+TGG | - | chr5.2:1986896-1986915 | MS.gene21756:CDS | 40.0% | |
| GCTACATCGGTAGAAATTGA+AGG | + | chr5.2:1987261-1987280 | None:intergenic | 40.0% | |
| GTGTTTCTCAAACGTGAGTT+TGG | + | chr5.2:1987088-1987107 | None:intergenic | 40.0% | |
| GTTTCATTAAACCACCCATC+TGG | + | chr5.2:1986961-1986980 | None:intergenic | 40.0% | |
| !! | GTTGGAGGCATTAACAAGAA+GGG | - | chr5.2:1987177-1987196 | MS.gene21756:CDS | 40.0% |
| !! | TGTTGGAGGCATTAACAAGA+AGG | - | chr5.2:1987176-1987195 | MS.gene21756:CDS | 40.0% |
| !! | TTGGAGGCATTAACAAGAAG+GGG | - | chr5.2:1987178-1987197 | MS.gene21756:CDS | 40.0% |
| !!! | GCTGGTTATGTTTTAGAGAG+TGG | - | chr5.2:1986873-1986892 | MS.gene21756:CDS | 40.0% |
| AACACTTCAATTCAGCCGGA+AGG | - | chr5.2:1987423-1987442 | MS.gene21756:intron | 45.0% | |
| AGACCAAAAGGGAAACCAGA+TGG | - | chr5.2:1986943-1986962 | MS.gene21756:CDS | 45.0% | |
| AGCGCTTGGAGCAAATGATT+GGG | - | chr5.2:1987382-1987401 | MS.gene21756:CDS | 45.0% | |
| CCATTATCACCTTCTCCTTC+CGG | + | chr5.2:1987441-1987460 | None:intergenic | 45.0% | |
| GACCAAAAGGGAAACCAGAT+GGG | - | chr5.2:1986944-1986963 | MS.gene21756:CDS | 45.0% | |
| TCGGTAGAAATTGAAGGCGA+TGG | + | chr5.2:1987255-1987274 | None:intergenic | 45.0% | |
| ! | AAAACTGAAGATGGAGCGCT+TGG | - | chr5.2:1987368-1987387 | MS.gene21756:CDS | 45.0% |
| ! | AACAAGAAGGGGCGCATATT+TGG | - | chr5.2:1987189-1987208 | MS.gene21756:CDS | 45.0% |
| ! | AAGGGGCGCATATTTGGATT+AGG | - | chr5.2:1987195-1987214 | MS.gene21756:CDS | 45.0% |
| CAAAAGGGAAACCAGATGGG+TGG | - | chr5.2:1986947-1986966 | MS.gene21756:CDS | 50.0% | |
| CCGGAAGGAGAAGGTGATAA+TGG | - | chr5.2:1987438-1987457 | MS.gene21756:intron | 50.0% | |
| GAGCGCTTGGAGCAAATGAT+TGG | - | chr5.2:1987381-1987400 | MS.gene21756:CDS | 50.0% | |
| TCAATTCAGCCGGAAGGAGA+AGG | - | chr5.2:1987429-1987448 | MS.gene21756:intron | 50.0% | |
| !! | CACCCATCTGGTTTCCCTTT+TGG | + | chr5.2:1986949-1986968 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 1986811 | 1987485 | 1986811 | ID=MS.gene21756 |
| chr5.2 | mRNA | 1986811 | 1987485 | 1986811 | ID=MS.gene21756.t1;Parent=MS.gene21756 |
| chr5.2 | exon | 1987483 | 1987485 | 1987483 | ID=MS.gene21756.t1.exon1;Parent=MS.gene21756.t1 |
| chr5.2 | CDS | 1987483 | 1987485 | 1987483 | ID=cds.MS.gene21756.t1;Parent=MS.gene21756.t1 |
| chr5.2 | exon | 1987296 | 1987407 | 1987296 | ID=MS.gene21756.t1.exon2;Parent=MS.gene21756.t1 |
| chr5.2 | CDS | 1987296 | 1987407 | 1987296 | ID=cds.MS.gene21756.t1;Parent=MS.gene21756.t1 |
| chr5.2 | exon | 1986811 | 1987235 | 1986811 | ID=MS.gene21756.t1.exon3;Parent=MS.gene21756.t1 |
| chr5.2 | CDS | 1986811 | 1987235 | 1986811 | ID=cds.MS.gene21756.t1;Parent=MS.gene21756.t1 |
| Gene Sequence |
| Protein sequence |
