Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057049.t1 | XP_024631911.1 | 72 | 168 | 12 | 1 | 1 | 133 | 416 | 583 | 1.40E-36 | 162.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057049.t1 | A0A072TGZ8 | 81.9 | 105 | 19 | 0 | 29 | 133 | 26 | 130 | 1.2e-21 | 112.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057049 | MS.gene062407 | 0.844489 | 7.32E-59 | -1.69E-46 |
| MS.gene057049 | MS.gene21756 | 0.82857 | 8.35E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057049.t1 | MTR_0125s0060 | 82.857 | 105 | 18 | 0 | 29 | 133 | 26 | 130 | 4.00e-57 | 174 |
| MS.gene057049.t1 | MTR_2g035110 | 76.699 | 103 | 24 | 0 | 36 | 138 | 33 | 135 | 1.37e-48 | 152 |
| MS.gene057049.t1 | MTR_8g066845 | 53.333 | 105 | 29 | 3 | 35 | 138 | 2 | 87 | 5.16e-23 | 86.3 |
| MS.gene057049.t1 | MTR_4g012560 | 70.968 | 62 | 13 | 1 | 51 | 112 | 24 | 80 | 2.57e-18 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCCATCTGGTTTCCCTTT+TGG | 0.170423 | 5.4:-69821438 | None:intergenic |
| AACAAGAAGGGGCGCTTATT+TGG | 0.255248 | 5.4:+69821681 | MS.gene057049:CDS |
| TGAATTGAAGTGTTCATATT+AGG | 0.299166 | 5.4:-69821906 | None:intergenic |
| CCATTATCACCTTCTCCTTC+CGG | 0.338322 | 5.4:-69821930 | None:intergenic |
| AAGGGGCGCTTATTTGGATT+AGG | 0.384291 | 5.4:+69821687 | MS.gene057049:CDS |
| GAGCGCTTGGAGCAAATGAT+TGG | 0.408396 | 5.4:+69821873 | MS.gene057049:CDS |
| CCGGAAGGAGAAGGTGATAA+TGG | 0.416904 | 5.4:+69821930 | MS.gene057049:CDS |
| GATAAACTTCATAGACCAAA+AGG | 0.428714 | 5.4:+69821423 | MS.gene057049:CDS |
| GTTTCATTAAACCACCCATC+TGG | 0.467331 | 5.4:-69821450 | None:intergenic |
| TTAGAGAGTGGAATTGGATA+GGG | 0.471643 | 5.4:+69821377 | MS.gene057049:intron |
| TTTAGAGAGTGGAATTGGAT+AGG | 0.476342 | 5.4:+69821376 | MS.gene057049:intron |
| AGAAACGCTAAAACTGAAGA+TGG | 0.499466 | 5.4:+69821851 | MS.gene057049:CDS |
| AAAACTGAAGATGGAGCGCT+TGG | 0.500382 | 5.4:+69821860 | MS.gene057049:CDS |
| TGTTGGAGGCATTAACAAGA+AGG | 0.515610 | 5.4:+69821668 | MS.gene057049:intron |
| AGCGCTTGGAGCAAATGATT+GGG | 0.526657 | 5.4:+69821874 | MS.gene057049:CDS |
| TATGAACACTTCAATTCAGC+CGG | 0.533653 | 5.4:+69821911 | MS.gene057049:CDS |
| TCAATTCAGCCGGAAGGAGA+AGG | 0.553755 | 5.4:+69821921 | MS.gene057049:CDS |
| GCTACATCGGTAGAAATTGA+AGG | 0.554924 | 5.4:-69821750 | None:intergenic |
| GTTGGAGGCATTAACAAGAA+GGG | 0.555037 | 5.4:+69821669 | MS.gene057049:intron |
| AGACCAAAAGGGAAACCAGA+TGG | 0.568337 | 5.4:+69821435 | MS.gene057049:CDS |
| GACCAAAAGGGAAACCAGAT+GGG | 0.568542 | 5.4:+69821436 | MS.gene057049:CDS |
| ATAAACTTCATAGACCAAAA+GGG | 0.570045 | 5.4:+69821424 | MS.gene057049:CDS |
| TCGGTAGAAATTGAAGGAGA+TGG | 0.579053 | 5.4:-69821744 | None:intergenic |
| TTGGAGGCATTAACAAGAAG+GGG | 0.580609 | 5.4:+69821670 | MS.gene057049:intron |
| TCGTAAATCTCAAACCATGT+TGG | 0.588016 | 5.4:-69821402 | None:intergenic |
| AACACTTCAATTCAGCCGGA+AGG | 0.621409 | 5.4:+69821915 | MS.gene057049:CDS |
| AATTGGATAGGGAACCAACA+TGG | 0.651250 | 5.4:+69821388 | MS.gene057049:CDS |
| CAAAAGGGAAACCAGATGGG+TGG | 0.669221 | 5.4:+69821439 | MS.gene057049:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGACAATGATATTTATA+TGG | + | chr5.4:69821641-69821660 | MS.gene057049:intron | 15.0% |
| !! | GATATTTATATGGAAATTGT+TGG | + | chr5.4:69821651-69821670 | MS.gene057049:intron | 20.0% |
| ! | ATAAACTTCATAGACCAAAA+GGG | + | chr5.4:69821424-69821443 | MS.gene057049:CDS | 25.0% |
| ! | ATTTATATGGAAATTGTTGG+AGG | + | chr5.4:69821654-69821673 | MS.gene057049:intron | 25.0% |
| !! | TGAATTGAAGTGTTCATATT+AGG | - | chr5.4:69821909-69821928 | None:intergenic | 25.0% |
| GATAAACTTCATAGACCAAA+AGG | + | chr5.4:69821423-69821442 | MS.gene057049:CDS | 30.0% | |
| TCATTGTCAATTTCTTCAGT+AGG | - | chr5.4:69821633-69821652 | None:intergenic | 30.0% | |
| TTCGAAAAAAGTTGCTTCTA+TGG | + | chr5.4:69821776-69821795 | MS.gene057049:CDS | 30.0% | |
| !!! | TATGTTTTAGAGAGTGGAAT+TGG | + | chr5.4:69821371-69821390 | MS.gene057049:intron | 30.0% |
| !!! | TTTTTTCGAAGAAGCTACAT+CGG | - | chr5.4:69821766-69821785 | None:intergenic | 30.0% |
| AGAAACGCTAAAACTGAAGA+TGG | + | chr5.4:69821851-69821870 | MS.gene057049:CDS | 35.0% | |
| GAAAAAAGTTGCTTCTATGG+AGG | + | chr5.4:69821779-69821798 | MS.gene057049:CDS | 35.0% | |
| TATGAACACTTCAATTCAGC+CGG | + | chr5.4:69821911-69821930 | MS.gene057049:CDS | 35.0% | |
| TCGTAAATCTCAAACCATGT+TGG | - | chr5.4:69821405-69821424 | None:intergenic | 35.0% | |
| TTAGAGAGTGGAATTGGATA+GGG | + | chr5.4:69821377-69821396 | MS.gene057049:intron | 35.0% | |
| TTTAGAGAGTGGAATTGGAT+AGG | + | chr5.4:69821376-69821395 | MS.gene057049:intron | 35.0% | |
| TTTGAGAAACACTCTCAAAG+TGG | + | chr5.4:69821588-69821607 | MS.gene057049:intron | 35.0% | |
| ! | GTTTCTCAAACGTTTGAGTT+TGG | - | chr5.4:69821578-69821597 | None:intergenic | 35.0% |
| !! | CATACTAACTTGTTGTTTGC+TGG | + | chr5.4:69821347-69821366 | MS.gene057049:intron | 35.0% |
| AATTGGATAGGGAACCAACA+TGG | + | chr5.4:69821388-69821407 | MS.gene057049:CDS | 40.0% | |
| GCTACATCGGTAGAAATTGA+AGG | - | chr5.4:69821753-69821772 | None:intergenic | 40.0% | |
| GTTTCATTAAACCACCCATC+TGG | - | chr5.4:69821453-69821472 | None:intergenic | 40.0% | |
| TCGGTAGAAATTGAAGGAGA+TGG | - | chr5.4:69821747-69821766 | None:intergenic | 40.0% | |
| !! | GTTGGAGGCATTAACAAGAA+GGG | + | chr5.4:69821669-69821688 | MS.gene057049:intron | 40.0% |
| !! | TGTTGGAGGCATTAACAAGA+AGG | + | chr5.4:69821668-69821687 | MS.gene057049:intron | 40.0% |
| !! | TTGGAGGCATTAACAAGAAG+GGG | + | chr5.4:69821670-69821689 | MS.gene057049:intron | 40.0% |
| !!! | GCTGGTTATGTTTTAGAGAG+TGG | + | chr5.4:69821365-69821384 | MS.gene057049:intron | 40.0% |
| AACACTTCAATTCAGCCGGA+AGG | + | chr5.4:69821915-69821934 | MS.gene057049:CDS | 45.0% | |
| AGACCAAAAGGGAAACCAGA+TGG | + | chr5.4:69821435-69821454 | MS.gene057049:CDS | 45.0% | |
| AGCGCTTGGAGCAAATGATT+GGG | + | chr5.4:69821874-69821893 | MS.gene057049:CDS | 45.0% | |
| CCATTATCACCTTCTCCTTC+CGG | - | chr5.4:69821933-69821952 | None:intergenic | 45.0% | |
| GACCAAAAGGGAAACCAGAT+GGG | + | chr5.4:69821436-69821455 | MS.gene057049:CDS | 45.0% | |
| ! | AAAACTGAAGATGGAGCGCT+TGG | + | chr5.4:69821860-69821879 | MS.gene057049:CDS | 45.0% |
| !! | AACAAGAAGGGGCGCTTATT+TGG | + | chr5.4:69821681-69821700 | MS.gene057049:CDS | 45.0% |
| !! | AAGGGGCGCTTATTTGGATT+AGG | + | chr5.4:69821687-69821706 | MS.gene057049:CDS | 45.0% |
| CAAAAGGGAAACCAGATGGG+TGG | + | chr5.4:69821439-69821458 | MS.gene057049:CDS | 50.0% | |
| CCGGAAGGAGAAGGTGATAA+TGG | + | chr5.4:69821930-69821949 | MS.gene057049:CDS | 50.0% | |
| GAGCGCTTGGAGCAAATGAT+TGG | + | chr5.4:69821873-69821892 | MS.gene057049:CDS | 50.0% | |
| TCAATTCAGCCGGAAGGAGA+AGG | + | chr5.4:69821921-69821940 | MS.gene057049:CDS | 50.0% | |
| !! | CACCCATCTGGTTTCCCTTT+TGG | - | chr5.4:69821441-69821460 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 69821303 | 69821977 | 69821303 | ID=MS.gene057049 |
| chr5.4 | mRNA | 69821303 | 69821977 | 69821303 | ID=MS.gene057049.t1;Parent=MS.gene057049 |
| chr5.4 | exon | 69821303 | 69821305 | 69821303 | ID=MS.gene057049.t1.exon1;Parent=MS.gene057049.t1 |
| chr5.4 | CDS | 69821303 | 69821305 | 69821303 | ID=cds.MS.gene057049.t1;Parent=MS.gene057049.t1 |
| chr5.4 | exon | 69821381 | 69821492 | 69821381 | ID=MS.gene057049.t1.exon2;Parent=MS.gene057049.t1 |
| chr5.4 | CDS | 69821381 | 69821492 | 69821381 | ID=cds.MS.gene057049.t1;Parent=MS.gene057049.t1 |
| chr5.4 | exon | 69821676 | 69821977 | 69821676 | ID=MS.gene057049.t1.exon3;Parent=MS.gene057049.t1 |
| chr5.4 | CDS | 69821676 | 69821977 | 69821676 | ID=cds.MS.gene057049.t1;Parent=MS.gene057049.t1 |
| Gene Sequence |
| Protein sequence |
