Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22318.t1 | XP_003611012.1 | 97 | 99 | 3 | 0 | 6 | 104 | 93 | 191 | 3.30E-43 | 184.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22318.t1 | G7KAM4 | 97.0 | 99 | 3 | 0 | 6 | 104 | 93 | 191 | 2.4e-43 | 184.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050759 | MS.gene22318 | 0.814196 | 1.74E-51 | -1.69E-46 |
| MS.gene050962 | MS.gene22318 | 0.805282 | 1.44E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22318.t1 | MTR_5g009490 | 96.154 | 104 | 4 | 0 | 1 | 104 | 88 | 191 | 3.28e-68 | 202 |
| MS.gene22318.t1 | MTR_8g099340 | 46.729 | 107 | 51 | 4 | 2 | 104 | 71 | 175 | 5.31e-18 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCAAAGAGTGTTTGTATA+TGG | 0.256621 | 5.4:-4121482 | MS.gene22318:CDS |
| AACCATATACAAACACTCTT+TGG | 0.339260 | 5.4:+4121480 | None:intergenic |
| ATCTCAAATTCAGGAACTTC+AGG | 0.352303 | 5.4:+4121424 | None:intergenic |
| TATATGGTTTATGATAAGTC+TGG | 0.403894 | 5.4:-4121466 | MS.gene22318:CDS |
| AGAACACCAATCTCAAATTC+AGG | 0.405479 | 5.4:+4121415 | None:intergenic |
| CGGTTCACGACCACCACAAT+TGG | 0.418761 | 5.4:-4121349 | MS.gene22318:CDS |
| TTTGAAACTATTGCTGCTGC+TGG | 0.434551 | 5.4:+4121519 | None:intergenic |
| GAAGTTCCTGAATTTGAGAT+TGG | 0.459665 | 5.4:-4121421 | MS.gene22318:CDS |
| TCAGGTATAGTAGAAAGAAC+TGG | 0.526063 | 5.4:+4121442 | None:intergenic |
| TTAACCAAGGAAAATACCTC+AGG | 0.528566 | 5.4:+4121388 | None:intergenic |
| AACGTTTCAAGCAGTATCAC+TGG | 0.562635 | 5.4:-4121544 | MS.gene22318:CDS |
| GACGCACGCAATACCAATTG+TGG | 0.574615 | 5.4:+4121336 | None:intergenic |
| AAAGAACGAGAAATCTTGTA+CGG | 0.579520 | 5.4:+4121613 | None:intergenic |
| TTAAAAGGTCAGCTTCTGAT+CGG | 0.581046 | 5.4:-4121369 | MS.gene22318:CDS |
| GATTGGTGTTCTTTCGCCTG+AGG | 0.584037 | 5.4:-4121404 | MS.gene22318:CDS |
| AGTGATACTGCTTGAAACGT+TGG | 0.596196 | 5.4:+4121546 | None:intergenic |
| GCACGCAATACCAATTGTGG+TGG | 0.661029 | 5.4:+4121339 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATATGGTTTATGATAAGTC+TGG | - | chr5.4:4121492-4121511 | MS.gene22318:CDS | 25.0% |
| AAAGAACGAGAAATCTTGTA+CGG | + | chr5.4:4121348-4121367 | None:intergenic | 30.0% | |
| AACCATATACAAACACTCTT+TGG | + | chr5.4:4121481-4121500 | None:intergenic | 30.0% | |
| ! | AGGTATTTTCCTTGGTTAAA+AGG | - | chr5.4:4121574-4121593 | MS.gene22318:CDS | 30.0% |
| AGAACACCAATCTCAAATTC+AGG | + | chr5.4:4121546-4121565 | None:intergenic | 35.0% | |
| ATCTCAAATTCAGGAACTTC+AGG | + | chr5.4:4121537-4121556 | None:intergenic | 35.0% | |
| TCAGGTATAGTAGAAAGAAC+TGG | + | chr5.4:4121519-4121538 | None:intergenic | 35.0% | |
| TTAAAAGGTCAGCTTCTGAT+CGG | - | chr5.4:4121589-4121608 | MS.gene22318:CDS | 35.0% | |
| TTAACCAAGGAAAATACCTC+AGG | + | chr5.4:4121573-4121592 | None:intergenic | 35.0% | |
| ! | GAAGTTCCTGAATTTGAGAT+TGG | - | chr5.4:4121537-4121556 | MS.gene22318:CDS | 35.0% |
| !! | CTCCAAAGAGTGTTTGTATA+TGG | - | chr5.4:4121476-4121495 | MS.gene22318:CDS | 35.0% |
| !!! | ATTCGTTCAGTTTTCAAGTC+AGG | - | chr5.4:4121378-4121397 | MS.gene22318:CDS | 35.0% |
| AACGTTTCAAGCAGTATCAC+TGG | - | chr5.4:4121414-4121433 | MS.gene22318:CDS | 40.0% | |
| AGTGATACTGCTTGAAACGT+TGG | + | chr5.4:4121415-4121434 | None:intergenic | 40.0% | |
| ! | AGAAGCTGACCTTTTAACCA+AGG | + | chr5.4:4121586-4121605 | None:intergenic | 40.0% |
| ! | TTTGAAACTATTGCTGCTGC+TGG | + | chr5.4:4121442-4121461 | None:intergenic | 40.0% |
| !! | TTCGCCTGAGGTATTTTCCT+TGG | - | chr5.4:4121566-4121585 | MS.gene22318:CDS | 45.0% |
| GACGCACGCAATACCAATTG+TGG | + | chr5.4:4121625-4121644 | None:intergenic | 50.0% | |
| GCACGCAATACCAATTGTGG+TGG | + | chr5.4:4121622-4121641 | None:intergenic | 50.0% | |
| ! | GATTGGTGTTCTTTCGCCTG+AGG | - | chr5.4:4121554-4121573 | MS.gene22318:CDS | 50.0% |
| CGGTTCACGACCACCACAAT+TGG | - | chr5.4:4121609-4121628 | MS.gene22318:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 4121333 | 4121647 | 4121333 | ID=MS.gene22318 |
| chr5.4 | mRNA | 4121333 | 4121647 | 4121333 | ID=MS.gene22318.t1;Parent=MS.gene22318 |
| chr5.4 | exon | 4121333 | 4121647 | 4121333 | ID=MS.gene22318.t1.exon1;Parent=MS.gene22318.t1 |
| chr5.4 | CDS | 4121333 | 4121647 | 4121333 | ID=cds.MS.gene22318.t1;Parent=MS.gene22318.t1 |
| Gene Sequence |
| Protein sequence |
