Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22449.t1 | XP_013449980.1 | 100 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 2.70E-23 | 117.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22449.t1 | P42552 | 76.2 | 63 | 15 | 0 | 11 | 73 | 8 | 70 | 2.5e-14 | 79.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22449.t1 | A0A072U4F5 | 100.0 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 1.9e-23 | 117.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene22449.t1 | TF | S1Fa-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene22449 | -0.806122 | 9.59E-50 | -1.69E-46 |
| MS.gene055539 | MS.gene22449 | -0.80091 | 1.15E-48 | -1.69E-46 |
| MS.gene058120 | MS.gene22449 | -0.813211 | 2.87E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22449.t1 | MTR_7g100905 | 100.000 | 73 | 0 | 0 | 1 | 73 | 1 | 73 | 9.50e-47 | 143 |
| MS.gene22449.t1 | MTR_7g100905 | 100.000 | 66 | 0 | 0 | 8 | 73 | 3 | 68 | 3.29e-41 | 129 |
| MS.gene22449.t1 | MTR_7g100880 | 85.897 | 78 | 6 | 1 | 1 | 73 | 7 | 84 | 2.78e-18 | 71.6 |
| MS.gene22449.t1 | MTR_7g100890 | 90.411 | 73 | 6 | 1 | 1 | 73 | 426 | 497 | 2.80e-16 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22449.t1 | AT2G37120 | 76.190 | 63 | 15 | 0 | 11 | 73 | 14 | 76 | 5.32e-15 | 63.2 |
| MS.gene22449.t1 | AT3G53370 | 80.000 | 60 | 12 | 0 | 14 | 73 | 17 | 76 | 1.71e-12 | 57.0 |
Find 16 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATTGTTCTCCTGGTTAT+TGG | 0.231388 | 7.1:-8408132 | MS.gene22449:CDS |
| CCCAGGCTTGATTGTTCTCC+TGG | 0.358351 | 7.1:-8408139 | MS.gene22449:CDS |
| TGGGGAAATGTTTAGAATGC+TGG | 0.422488 | 7.1:-8408177 | MS.gene22449:intron |
| GTTTAGAATGCTGGATCTCA+AGG | 0.468079 | 7.1:-8408168 | MS.gene22449:intron |
| ACCAGGAGAACAATCAAGCC+TGG | 0.470447 | 7.1:+8408138 | None:intergenic |
| ATTGTTCTCCTGGTTATTGG+TGG | 0.489076 | 7.1:-8408129 | MS.gene22449:CDS |
| TTGCTGTTGACATTTCTCAT+TGG | 0.497549 | 7.1:-8408105 | MS.gene22449:CDS |
| AAGCAAGGAGTCTCTGCACC+TGG | 0.518649 | 7.1:-8407991 | MS.gene22449:CDS |
| GTACAGTGCCTCTACTCTCC+AGG | 0.535553 | 7.1:+8407973 | None:intergenic |
| TTGTTCTCCTGGTTATTGGT+GGG | 0.546594 | 7.1:-8408128 | MS.gene22449:CDS |
| GCTTTACGTTTATGCACAGA+AGG | 0.577488 | 7.1:-8408073 | MS.gene22449:CDS |
| ACAGCAACCCACCAATAACC+AGG | 0.598951 | 7.1:+8408121 | None:intergenic |
| CCAGGAGAACAATCAAGCCT+GGG | 0.608678 | 7.1:+8408139 | None:intergenic |
| GGATCTCAAGGGTTCAACCC+AGG | 0.613072 | 7.1:-8408156 | MS.gene22449:CDS |
| TTTAGAATGCTGGATCTCAA+GGG | 0.619147 | 7.1:-8408167 | MS.gene22449:intron |
| TCTCTGCACCTGGAGAGTAG+AGG | 0.653251 | 7.1:-8407981 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTTTTATTCACATTT+AGG | - | chr7.1:8408250-8408269 | MS.gene22449:intron | 10.0% |
| !! | AAAATAAATAATGAAGAATG+TGG | + | chr7.1:8408055-8408074 | None:intergenic | 15.0% |
| !! | AAATAAATAATGAAGAATGT+GGG | + | chr7.1:8408054-8408073 | None:intergenic | 15.0% |
| !! | CAATAAAAAAATATTTCCAA+AGG | + | chr7.1:8409029-8409048 | None:intergenic | 15.0% |
| !! | GATCTATTCATAATTAAATT+AGG | - | chr7.1:8408225-8408244 | MS.gene22449:intron | 15.0% |
| !! | TTATTTGAGAATAACAATAA+GGG | + | chr7.1:8408620-8408639 | None:intergenic | 15.0% |
| !! | AGATTCTACTTTGAATATAA+TGG | - | chr7.1:8408843-8408862 | MS.gene22449:intron | 20.0% |
| !! | CTTATTTGAGAATAACAATA+AGG | + | chr7.1:8408621-8408640 | None:intergenic | 20.0% |
| !! | GATTCTACTTTGAATATAAT+GGG | - | chr7.1:8408844-8408863 | MS.gene22449:intron | 20.0% |
| !! | TTTCAGATCTTAAAAATTCA+TGG | - | chr7.1:8408074-8408093 | MS.gene22449:CDS | 20.0% |
| !!! | CAAAAAAAAGCACTTAAATA+AGG | + | chr7.1:8409072-8409091 | None:intergenic | 20.0% |
| !!! | TTTTATTCACATTTAGGTTA+AGG | - | chr7.1:8408256-8408275 | MS.gene22449:intron | 20.0% |
| ! | AAAATGCATATGATAAAACC+TGG | + | chr7.1:8408576-8408595 | None:intergenic | 25.0% |
| ! | CATATTACTATTTGCATACA+TGG | - | chr7.1:8408961-8408980 | MS.gene22449:intron | 25.0% |
| ! | CTCAAAAAAGAAGATGAAAA+AGG | - | chr7.1:8409254-8409273 | MS.gene22449:intron | 25.0% |
| ! | GGTTACAAATAACATCAATA+TGG | + | chr7.1:8408599-8408618 | None:intergenic | 25.0% |
| !! | TTTACATGTCAATTTTCCTT+TGG | - | chr7.1:8409010-8409029 | MS.gene22449:intron | 25.0% |
| !!! | GGAATTGATTTGAAGAAAAT+CGG | + | chr7.1:8408033-8408052 | None:intergenic | 25.0% |
| !!! | TGCTTTATTAGACACTTTTA+AGG | - | chr7.1:8408329-8408348 | MS.gene22449:intron | 25.0% |
| !!! | TTAATTTGAGAGTGTTTTTG+TGG | + | chr7.1:8408782-8408801 | None:intergenic | 25.0% |
| AAAAGAAGATGAAAAAGGAG+AGG | - | chr7.1:8409259-8409278 | MS.gene22449:intron | 30.0% | |
| AACCATTCAACACAAATTGA+TGG | + | chr7.1:8408551-8408570 | None:intergenic | 30.0% | |
| ATTCTCAAATAAGTCTGTGT+AGG | - | chr7.1:8408628-8408647 | MS.gene22449:intron | 30.0% | |
| ATTGCAGAGCAAATAAAGAA+TGG | + | chr7.1:8408940-8408959 | None:intergenic | 30.0% | |
| TATGAATAGATCCACAAGTA+AGG | + | chr7.1:8408217-8408236 | None:intergenic | 30.0% | |
| ! | TTTTGTGGAGACATACAAAA+GGG | + | chr7.1:8408767-8408786 | None:intergenic | 30.0% |
| ! | TTTTTGTGGAGACATACAAA+AGG | + | chr7.1:8408768-8408787 | None:intergenic | 30.0% |
| !!! | AATTTTTTACCTCCATCACT+GGG | - | chr7.1:8408174-8408193 | MS.gene22449:intron | 30.0% |
| !!! | TAATTTTTTACCTCCATCAC+TGG | - | chr7.1:8408173-8408192 | MS.gene22449:intron | 30.0% |
| !!! | TGCTTTTTTTTGTTGCTCTT+GGG | - | chr7.1:8409080-8409099 | MS.gene22449:intron | 30.0% |
| !!! | TTCATCTTCTTTTTTGAGAC+TGG | + | chr7.1:8409252-8409271 | None:intergenic | 30.0% |
| AGAAAATCGGATCTAAGAAG+AGG | + | chr7.1:8408020-8408039 | None:intergenic | 35.0% | |
| AGGGCATAGAATATGAGAAA+GGG | + | chr7.1:8408116-8408135 | None:intergenic | 35.0% | |
| AGTTATATACGCCTTACTTG+TGG | - | chr7.1:8408203-8408222 | MS.gene22449:intron | 35.0% | |
| ATAACTGAAAAACCCAGTGA+TGG | + | chr7.1:8408189-8408208 | None:intergenic | 35.0% | |
| ATTGTATTGTACACGCGTTA+AGG | - | chr7.1:8408668-8408687 | MS.gene22449:intron | 35.0% | |
| TGCCATCAATTTGTGTTGAA+TGG | - | chr7.1:8408546-8408565 | MS.gene22449:intron | 35.0% | |
| TTGATTGTTCTCCTGGTTAT+TGG | - | chr7.1:8409144-8409163 | MS.gene22449:intron | 35.0% | |
| ! | AAGGGCATAGAATATGAGAA+AGG | + | chr7.1:8408117-8408136 | None:intergenic | 35.0% |
| ! | AAGTAGAATCTCTGATTTGC+AGG | + | chr7.1:8408834-8408853 | None:intergenic | 35.0% |
| ! | CCTTTGTTTCAAAGAATCTC+TGG | - | chr7.1:8408882-8408901 | MS.gene22449:intron | 35.0% |
| ! | GATCATGATGATGATGATGA+TGG | - | chr7.1:8408442-8408461 | MS.gene22449:intron | 35.0% |
| ! | TCAACACAAATTGATGGCAT+TGG | + | chr7.1:8408545-8408564 | None:intergenic | 35.0% |
| ! | TGAAAGTATGAACGAGCAAA+GGG | + | chr7.1:8408135-8408154 | None:intergenic | 35.0% |
| ! | TTGAAAGTATGAACGAGCAA+AGG | + | chr7.1:8408136-8408155 | None:intergenic | 35.0% |
| ! | TTGCTGTTGACATTTCTCAT+TGG | - | chr7.1:8409171-8409190 | MS.gene22449:intron | 35.0% |
| ! | TTTAGAATGCTGGATCTCAA+GGG | - | chr7.1:8409109-8409128 | MS.gene22449:intron | 35.0% |
| ! | TTTGAAACAAAGGAGAGAAG+TGG | + | chr7.1:8408875-8408894 | None:intergenic | 35.0% |
| ! | TTTGTGTTGAATGGTTTTCC+AGG | - | chr7.1:8408555-8408574 | MS.gene22449:intron | 35.0% |
| !! | CCAGAGATTCTTTGAAACAA+AGG | + | chr7.1:8408885-8408904 | None:intergenic | 35.0% |
| !!! | GCTTTTTTTTGTTGCTCTTG+GGG | - | chr7.1:8409081-8409100 | MS.gene22449:intron | 35.0% |
| !!! | GTGCTTTTTTTTGTTGCTCT+TGG | - | chr7.1:8409079-8409098 | MS.gene22449:intron | 35.0% |
| AGAAGAGGAGAGAAGAATAC+CGG | + | chr7.1:8408005-8408024 | None:intergenic | 40.0% | |
| ATTGTTCTCCTGGTTATTGG+TGG | - | chr7.1:8409147-8409166 | MS.gene22449:intron | 40.0% | |
| CATACATGGATGATTGTGAG+TGG | - | chr7.1:8408975-8408994 | MS.gene22449:intron | 40.0% | |
| GCTTTACGTTTATGCACAGA+AGG | - | chr7.1:8409203-8409222 | MS.gene22449:intron | 40.0% | |
| GGGACTGTGAATCATAAATC+AGG | + | chr7.1:8408747-8408766 | None:intergenic | 40.0% | |
| GGGCATAGAATATGAGAAAG+GGG | + | chr7.1:8408115-8408134 | None:intergenic | 40.0% | |
| TTGTTCTCCTGGTTATTGGT+GGG | - | chr7.1:8409148-8409167 | MS.gene22449:intron | 40.0% | |
| ! | ATGATGGTGATACTGCAGAA+TGG | - | chr7.1:8408458-8408477 | MS.gene22449:intron | 40.0% |
| ! | GTTTAGAATGCTGGATCTCA+AGG | - | chr7.1:8409108-8409127 | MS.gene22449:intron | 40.0% |
| ! | TGGGGAAATGTTTAGAATGC+TGG | - | chr7.1:8409099-8409118 | MS.gene22449:intron | 40.0% |
| !!! | ACTGGCTTCTTTTTTCTAGG+AGG | + | chr7.1:8409234-8409253 | None:intergenic | 40.0% |
| !!! | CTGGCTTCTTTTTTCTAGGA+GGG | + | chr7.1:8409233-8409252 | None:intergenic | 40.0% |
| !!! | GAGACTGGCTTCTTTTTTCT+AGG | + | chr7.1:8409237-8409256 | None:intergenic | 40.0% |
| AAAAAGGAGAGGCTGAAGCA+AGG | - | chr7.1:8409270-8409289 | MS.gene22449:intron | 45.0% | |
| AAGAGGAGAGAAGAATACCG+GGG | + | chr7.1:8408003-8408022 | None:intergenic | 45.0% | |
| ACATGGATGATTGTGAGTGG+TGG | - | chr7.1:8408978-8408997 | MS.gene22449:intron | 45.0% | |
| ACTGAAAAACCCAGTGATGG+AGG | + | chr7.1:8408186-8408205 | None:intergenic | 45.0% | |
| GAAGAGGAGAGAAGAATACC+GGG | + | chr7.1:8408004-8408023 | None:intergenic | 45.0% | |
| ! | ATATGAGAAAGGGGCATGCA+AGG | + | chr7.1:8408106-8408125 | None:intergenic | 45.0% |
| ! | TATGAGAAAGGGGCATGCAA+GGG | + | chr7.1:8408105-8408124 | None:intergenic | 45.0% |
| ACAGCAACCCACCAATAACC+AGG | + | chr7.1:8409158-8409177 | None:intergenic | 50.0% | |
| ACCAGGAGAACAATCAAGCC+TGG | + | chr7.1:8409141-8409160 | None:intergenic | 50.0% | |
| AGAGGAGAGAAGAATACCGG+GGG | + | chr7.1:8408002-8408021 | None:intergenic | 50.0% | |
| CCAGGAGAACAATCAAGCCT+GGG | + | chr7.1:8409140-8409159 | None:intergenic | 50.0% | |
| GAATACCGGGGGGACTTTAT+CGG | + | chr7.1:8407991-8408010 | None:intergenic | 50.0% | |
| AAGCAAGGAGTCTCTGCACC+TGG | - | chr7.1:8409285-8409304 | MS.gene22449:intron | 55.0% | |
| CCCAGGCTTGATTGTTCTCC+TGG | - | chr7.1:8409137-8409156 | MS.gene22449:intron | 55.0% | |
| GAGGAGAGAAGAATACCGGG+GGG | + | chr7.1:8408001-8408020 | None:intergenic | 55.0% | |
| GGATCTCAAGGGTTCAACCC+AGG | - | chr7.1:8409120-8409139 | MS.gene22449:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 8407984 | 8409314 | 8407984 | ID=MS.gene22449 |
| chr7.1 | mRNA | 8407984 | 8409314 | 8407984 | ID=MS.gene22449.t1;Parent=MS.gene22449 |
| chr7.1 | exon | 8409294 | 8409314 | 8409294 | ID=MS.gene22449.t1.exon1;Parent=MS.gene22449.t1 |
| chr7.1 | CDS | 8409294 | 8409314 | 8409294 | ID=cds.MS.gene22449.t1;Parent=MS.gene22449.t1 |
| chr7.1 | exon | 8407984 | 8408184 | 8407984 | ID=MS.gene22449.t1.exon2;Parent=MS.gene22449.t1 |
| chr7.1 | CDS | 8407984 | 8408184 | 8407984 | ID=cds.MS.gene22449.t1;Parent=MS.gene22449.t1 |
| Gene Sequence |
| Protein sequence |
