Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22741.t1 | GAU13696.1 | 51.8 | 137 | 37 | 5 | 1 | 108 | 1 | 137 | 1.30E-21 | 112.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22741.t1 | Q2HTD5 | 54.0 | 126 | 42 | 4 | 1 | 111 | 1 | 125 | 2.7e-21 | 110.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049805 | MS.gene22741 | 0.815839 | 7.53E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22741.t1 | MTR_7g118130 | 58.730 | 126 | 36 | 4 | 1 | 111 | 1 | 125 | 1.42e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTCCAATTCATCCAATT+TGG | 0.162116 | 7.4:-572595 | None:intergenic |
| CGATCATTGGGGTCCCAAAT+TGG | 0.282259 | 7.4:+572581 | MS.gene22741:CDS |
| TTCTCCAATTCATCCAATTT+GGG | 0.339109 | 7.4:-572594 | None:intergenic |
| CACTCTGTATAAACGATCAT+TGG | 0.347459 | 7.4:+572568 | MS.gene22741:CDS |
| GGGTCCCAAATTGGATGAAT+TGG | 0.357124 | 7.4:+572590 | MS.gene22741:CDS |
| AGTGGGTATTCAGGCCAATA+TGG | 0.382608 | 7.4:+572404 | MS.gene22741:CDS |
| GCATCATCGAGTGGGTATTC+AGG | 0.385134 | 7.4:+572395 | MS.gene22741:CDS |
| AGGAGTCTCTTCCATGCGAC+TGG | 0.421499 | 7.4:-572523 | None:intergenic |
| ACTAATCGATGATTCCATAT+TGG | 0.431208 | 7.4:-572418 | None:intergenic |
| ACTCTGTATAAACGATCATT+GGG | 0.435834 | 7.4:+572569 | MS.gene22741:CDS |
| CTCCTCTGCGCCCAGTCGCA+TGG | 0.454617 | 7.4:+572512 | MS.gene22741:CDS |
| CCGCCGTGATTGTAGAGAAC+TGG | 0.470912 | 7.4:-572370 | None:intergenic |
| CTCAGCAGCACACGTTGTCT+TGG | 0.473627 | 7.4:+572344 | MS.gene22741:CDS |
| AGCAGCACACGTTGTCTTGG+CGG | 0.509222 | 7.4:+572347 | MS.gene22741:CDS |
| CATGCGACTGGGCGCAGAGG+AGG | 0.514470 | 7.4:-572511 | None:intergenic |
| TAGTGGGTCAGATGTATTGT+GGG | 0.536796 | 7.4:+572437 | MS.gene22741:CDS |
| ATCAAAGAGTATGTTCTGAA+AGG | 0.563818 | 7.4:-572629 | None:intergenic |
| TTCCATGCGACTGGGCGCAG+AGG | 0.567243 | 7.4:-572514 | None:intergenic |
| CGAACAATAACATCGGTATG+AGG | 0.567548 | 7.4:-572543 | None:intergenic |
| GTTCTCTACAATCACGGCGG+CGG | 0.568781 | 7.4:+572373 | MS.gene22741:CDS |
| AATATGGAATCATCGATTAG+TGG | 0.570871 | 7.4:+572420 | MS.gene22741:CDS |
| GGAGTCTCTTCCATGCGACT+GGG | 0.571081 | 7.4:-572522 | None:intergenic |
| ACGGCGGCGGCATCATCGAG+TGG | 0.593713 | 7.4:+572386 | MS.gene22741:CDS |
| ATATGGAATCATCGATTAGT+GGG | 0.595651 | 7.4:+572421 | MS.gene22741:CDS |
| CGGCCAGTTCTCTACAATCA+CGG | 0.600978 | 7.4:+572367 | MS.gene22741:CDS |
| AATGATCGTTTATACAGAGT+GGG | 0.619057 | 7.4:-572566 | None:intergenic |
| CAATGATCGTTTATACAGAG+TGG | 0.623120 | 7.4:-572567 | None:intergenic |
| AAAAGGAAAAGATGTGGTGT+TGG | 0.624449 | 7.4:+572304 | None:intergenic |
| CGGCGGCGGCATCATCGAGT+GGG | 0.630862 | 7.4:+572387 | MS.gene22741:CDS |
| TCGTAAGATCAAGATCAAGA+AGG | 0.637303 | 7.4:+572482 | MS.gene22741:CDS |
| TTAGTGGGTCAGATGTATTG+TGG | 0.640580 | 7.4:+572436 | MS.gene22741:CDS |
| TGCTGCTGAGATGACAACGT+TGG | 0.701374 | 7.4:-572331 | None:intergenic |
| CTCTGTATAAACGATCATTG+GGG | 0.729290 | 7.4:+572570 | MS.gene22741:CDS |
| GAGTGGGCGAACAATAACAT+CGG | 0.729674 | 7.4:-572550 | None:intergenic |
| CCAGTTCTCTACAATCACGG+CGG | 0.772030 | 7.4:+572370 | MS.gene22741:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGATCGTTTATACAGAGT+GGG | - | chr7.4:572569-572588 | None:intergenic | 30.0% | |
| ACTAATCGATGATTCCATAT+TGG | - | chr7.4:572421-572440 | None:intergenic | 30.0% | |
| ACTCTGTATAAACGATCATT+GGG | + | chr7.4:572569-572588 | MS.gene22741:CDS | 30.0% | |
| TTCTCCAATTCATCCAATTT+GGG | - | chr7.4:572597-572616 | None:intergenic | 30.0% | |
| TTTCTCCAATTCATCCAATT+TGG | - | chr7.4:572598-572617 | None:intergenic | 30.0% | |
| ! | AATATGGAATCATCGATTAG+TGG | + | chr7.4:572420-572439 | MS.gene22741:CDS | 30.0% |
| ! | ATATGGAATCATCGATTAGT+GGG | + | chr7.4:572421-572440 | MS.gene22741:CDS | 30.0% |
| CAATGATCGTTTATACAGAG+TGG | - | chr7.4:572570-572589 | None:intergenic | 35.0% | |
| CACTCTGTATAAACGATCAT+TGG | + | chr7.4:572568-572587 | MS.gene22741:CDS | 35.0% | |
| CTCTGTATAAACGATCATTG+GGG | + | chr7.4:572570-572589 | MS.gene22741:CDS | 35.0% | |
| TCGTAAGATCAAGATCAAGA+AGG | + | chr7.4:572482-572501 | MS.gene22741:CDS | 35.0% | |
| CGAACAATAACATCGGTATG+AGG | - | chr7.4:572546-572565 | None:intergenic | 40.0% | |
| ! | TAGTGGGTCAGATGTATTGT+GGG | + | chr7.4:572437-572456 | MS.gene22741:CDS | 40.0% |
| ! | TTAGTGGGTCAGATGTATTG+TGG | + | chr7.4:572436-572455 | MS.gene22741:CDS | 40.0% |
| AGTGGGTATTCAGGCCAATA+TGG | + | chr7.4:572404-572423 | MS.gene22741:CDS | 45.0% | |
| GAGTGGGCGAACAATAACAT+CGG | - | chr7.4:572553-572572 | None:intergenic | 45.0% | |
| GGGTCCCAAATTGGATGAAT+TGG | + | chr7.4:572590-572609 | MS.gene22741:CDS | 45.0% | |
| CCAGTTCTCTACAATCACGG+CGG | + | chr7.4:572370-572389 | MS.gene22741:CDS | 50.0% | |
| CGATCATTGGGGTCCCAAAT+TGG | + | chr7.4:572581-572600 | MS.gene22741:CDS | 50.0% | |
| CGGCCAGTTCTCTACAATCA+CGG | + | chr7.4:572367-572386 | MS.gene22741:CDS | 50.0% | |
| GCATCATCGAGTGGGTATTC+AGG | + | chr7.4:572395-572414 | MS.gene22741:CDS | 50.0% | |
| TGCTGCTGAGATGACAACGT+TGG | - | chr7.4:572334-572353 | None:intergenic | 50.0% | |
| AGCAGCACACGTTGTCTTGG+CGG | + | chr7.4:572347-572366 | MS.gene22741:CDS | 55.0% | |
| AGGAGTCTCTTCCATGCGAC+TGG | - | chr7.4:572526-572545 | None:intergenic | 55.0% | |
| CCGCCGTGATTGTAGAGAAC+TGG | - | chr7.4:572373-572392 | None:intergenic | 55.0% | |
| CTCAGCAGCACACGTTGTCT+TGG | + | chr7.4:572344-572363 | MS.gene22741:CDS | 55.0% | |
| GGAGTCTCTTCCATGCGACT+GGG | - | chr7.4:572525-572544 | None:intergenic | 55.0% | |
| GTTCTCTACAATCACGGCGG+CGG | + | chr7.4:572373-572392 | MS.gene22741:CDS | 55.0% | |
| TTCCATGCGACTGGGCGCAG+AGG | - | chr7.4:572517-572536 | None:intergenic | 65.0% | |
| ACGGCGGCGGCATCATCGAG+TGG | + | chr7.4:572386-572405 | MS.gene22741:CDS | 70.0% | |
| CATGCGACTGGGCGCAGAGG+AGG | - | chr7.4:572514-572533 | None:intergenic | 70.0% | |
| CGGCGGCGGCATCATCGAGT+GGG | + | chr7.4:572387-572406 | MS.gene22741:CDS | 70.0% | |
| CTCCTCTGCGCCCAGTCGCA+TGG | + | chr7.4:572512-572531 | MS.gene22741:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 572315 | 572650 | 572315 | ID=MS.gene22741 |
| chr7.4 | mRNA | 572315 | 572650 | 572315 | ID=MS.gene22741.t1;Parent=MS.gene22741 |
| chr7.4 | exon | 572315 | 572650 | 572315 | ID=MS.gene22741.t1.exon1;Parent=MS.gene22741.t1 |
| chr7.4 | CDS | 572315 | 572650 | 572315 | ID=cds.MS.gene22741.t1;Parent=MS.gene22741.t1 |
| Gene Sequence |
| Protein sequence |
