Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22886.t1 | XP_003626375.2 | 97.1 | 137 | 4 | 0 | 1 | 137 | 69 | 205 | 8.70E-68 | 266.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22886.t1 | G7L0S2 | 97.1 | 137 | 4 | 0 | 1 | 137 | 69 | 205 | 6.2e-68 | 266.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051860 | MS.gene22886 | 0.812952 | 3.27E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene22886 | 0.80559 | 1.24E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene22886 | 0.803575 | 3.27E-49 | -1.69E-46 |
| MS.gene054473 | MS.gene22886 | 0.814338 | 1.62E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22886.t1 | MTR_7g114390 | 97.080 | 137 | 4 | 0 | 1 | 137 | 69 | 205 | 4.73e-97 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22886.t1 | AT3G04950 | 70.803 | 137 | 40 | 0 | 1 | 137 | 89 | 225 | 5.30e-73 | 218 |
Find 27 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAACTATATACTCTTTAA+AGG | 0.178788 | 7.3:+2425106 | None:intergenic |
| GGATATTGCAAGGTCTAATT+TGG | 0.200253 | 7.3:-2424223 | MS.gene22886:CDS |
| GATTTGTACCGACCATCTTT+TGG | 0.311365 | 7.3:+2424250 | None:intergenic |
| ATGCACTTGCAAAGTTTACT+TGG | 0.358285 | 7.3:-2424857 | MS.gene22886:CDS |
| GTTCAAAGAAGAGATATAAA+AGG | 0.388106 | 7.3:-2424158 | None:intergenic |
| GCCTTTAAAGAGTATATAGT+TGG | 0.433265 | 7.3:-2425107 | MS.gene22886:CDS |
| AATCGAATGCGGCGAGATAT+CGG | 0.442505 | 7.3:+2425030 | None:intergenic |
| ATGAAGAAAGCAAGAAAAGT+TGG | 0.472505 | 7.3:-2425131 | MS.gene22886:CDS |
| TTAGACCTTGCAATATCCAT+TGG | 0.473307 | 7.3:+2424228 | None:intergenic |
| TTCTTGTTAGCTCCAAAAGA+TGG | 0.497318 | 7.3:-2424262 | MS.gene22886:intron |
| CTAAAAGCGGACAAGTTAGC+AGG | 0.515399 | 7.3:-2424200 | MS.gene22886:CDS |
| AATGCCTTCGTATTTCTCAA+TGG | 0.521342 | 7.3:+2425055 | None:intergenic |
| TGCACTTGCAAAGTTTACTT+GGG | 0.524528 | 7.3:-2424856 | MS.gene22886:CDS |
| TAAGTGCACAATTGCTGAAG+TGG | 0.546585 | 7.3:-2424883 | MS.gene22886:CDS |
| ATTCAAAAGTTAAATCAAGA+AGG | 0.556894 | 7.3:-2424813 | MS.gene22886:CDS |
| TATCTCTTCTTTGAACCACA+TGG | 0.570464 | 7.3:+2424165 | None:intergenic |
| GATGGTCGGTACAAATCCAA+TGG | 0.571490 | 7.3:-2424244 | MS.gene22886:CDS |
| CTTTGAACCACATGGACAGA+GGG | 0.580056 | 7.3:+2424173 | None:intergenic |
| CAAATCCAATGGATATTGCA+AGG | 0.593492 | 7.3:-2424233 | MS.gene22886:CDS |
| CGCATTCGATTACACAGGAG+AGG | 0.602676 | 7.3:-2425018 | MS.gene22886:intron |
| AGGAATGCCCTCTGTCCATG+TGG | 0.628191 | 7.3:-2424180 | MS.gene22886:CDS |
| AGGTCTAATTTGGCTAAAAG+CGG | 0.628257 | 7.3:-2424213 | MS.gene22886:CDS |
| TGTTAGCTCCAAAAGATGGT+CGG | 0.634247 | 7.3:-2424258 | MS.gene22886:intron |
| TCTTTGAACCACATGGACAG+AGG | 0.637245 | 7.3:+2424172 | None:intergenic |
| CTCTCCTGTGTAATCGAATG+CGG | 0.644799 | 7.3:+2425019 | None:intergenic |
| ACTGCCATTGAGAAATACGA+AGG | 0.691670 | 7.3:-2425059 | MS.gene22886:CDS |
| CTCGCCGCATTCGATTACAC+AGG | 0.703771 | 7.3:-2425023 | MS.gene22886:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAATTAATAAAATGATGA+AGG | + | chr7.3:2424557-2424576 | None:intergenic | 10.0% |
| !! | ATTCAAAAGTTAAATCAAGA+AGG | - | chr7.3:2424477-2424496 | MS.gene22886:intron | 20.0% |
| !!! | ATTACTTCAGACATATTTTT+AGG | + | chr7.3:2424513-2424532 | None:intergenic | 20.0% |
| !!! | TTGTTGATTTTGTTTTGTTA+TGG | - | chr7.3:2424339-2424358 | MS.gene22886:intron | 20.0% |
| ! | ACAAGAAACATAAATATGGA+TGG | + | chr7.3:2425015-2425034 | None:intergenic | 25.0% |
| ! | GCTAACAAGAAACATAAATA+TGG | + | chr7.3:2425019-2425038 | None:intergenic | 25.0% |
| ! | TCCAACTATATACTCTTTAA+AGG | + | chr7.3:2424187-2424206 | None:intergenic | 25.0% |
| !!! | GATGAAGGTATTATTAAGTA+AGG | + | chr7.3:2424542-2424561 | None:intergenic | 25.0% |
| AACGAATCAAAAACATGAAG+CGG | - | chr7.3:2424374-2424393 | MS.gene22886:intron | 30.0% | |
| AATCACTCTCTAATTCTTCA+AGG | + | chr7.3:2424597-2424616 | None:intergenic | 30.0% | |
| ATCACTCTCTAATTCTTCAA+GGG | + | chr7.3:2424596-2424615 | None:intergenic | 30.0% | |
| ATGAAGAAAGCAAGAAAAGT+TGG | - | chr7.3:2424159-2424178 | MS.gene22886:CDS | 30.0% | |
| ATGTGTTTCTTGTTCTTGTA+AGG | - | chr7.3:2424969-2424988 | MS.gene22886:intron | 30.0% | |
| TGTGTTTCTTGTTCTTGTAA+GGG | - | chr7.3:2424970-2424989 | MS.gene22886:intron | 30.0% | |
| TTAACACGAATCAACATCAT+CGG | + | chr7.3:2424693-2424712 | None:intergenic | 30.0% | |
| TTTCGTTGTTTGCATGATTT+GGG | - | chr7.3:2424812-2424831 | MS.gene22886:CDS | 30.0% | |
| ! | GCCTTTAAAGAGTATATAGT+TGG | - | chr7.3:2424183-2424202 | MS.gene22886:CDS | 30.0% |
| !! | ATGATGTTGATTCGTGTTAA+TGG | - | chr7.3:2424693-2424712 | MS.gene22886:intron | 30.0% |
| !! | GAAGGTATTATTAAGTAAGG+AGG | + | chr7.3:2424539-2424558 | None:intergenic | 30.0% |
| !! | GTTTTGTTGTCTTTTGTTGT+TGG | - | chr7.3:2424765-2424784 | MS.gene22886:intron | 30.0% |
| !!! | TCAGACATATTTTTAGGCAT+GGG | + | chr7.3:2424507-2424526 | None:intergenic | 30.0% |
| !!! | TTCAGACATATTTTTAGGCA+TGG | + | chr7.3:2424508-2424527 | None:intergenic | 30.0% |
| AATGCCTTCGTATTTCTCAA+TGG | + | chr7.3:2424238-2424257 | None:intergenic | 35.0% | |
| AGGTCTAATTTGGCTAAAAG+CGG | - | chr7.3:2425077-2425096 | MS.gene22886:CDS | 35.0% | |
| ATGCACTTGCAAAGTTTACT+TGG | - | chr7.3:2424433-2424452 | MS.gene22886:intron | 35.0% | |
| CAAATCCAATGGATATTGCA+AGG | - | chr7.3:2425057-2425076 | MS.gene22886:CDS | 35.0% | |
| CTTTCGTTGTTTGCATGATT+TGG | - | chr7.3:2424811-2424830 | MS.gene22886:CDS | 35.0% | |
| GGATATTGCAAGGTCTAATT+TGG | - | chr7.3:2425067-2425086 | MS.gene22886:CDS | 35.0% | |
| TATCTCTTCTTTGAACCACA+TGG | + | chr7.3:2425128-2425147 | None:intergenic | 35.0% | |
| TGCACTTGCAAAGTTTACTT+GGG | - | chr7.3:2424434-2424453 | MS.gene22886:intron | 35.0% | |
| TTAGACCTTGCAATATCCAT+TGG | + | chr7.3:2425065-2425084 | None:intergenic | 35.0% | |
| TTCTTGTTAGCTCCAAAAGA+TGG | - | chr7.3:2425028-2425047 | MS.gene22886:CDS | 35.0% | |
| ACTGCCATTGAGAAATACGA+AGG | - | chr7.3:2424231-2424250 | MS.gene22886:CDS | 40.0% | |
| TAAGTGCACAATTGCTGAAG+TGG | - | chr7.3:2424407-2424426 | MS.gene22886:intron | 40.0% | |
| TGTTAGCTCCAAAAGATGGT+CGG | - | chr7.3:2425032-2425051 | MS.gene22886:CDS | 40.0% | |
| !! | GATTTGTACCGACCATCTTT+TGG | + | chr7.3:2425043-2425062 | None:intergenic | 40.0% |
| AATCGAATGCGGCGAGATAT+CGG | + | chr7.3:2424263-2424282 | None:intergenic | 45.0% | |
| CTAAAAGCGGACAAGTTAGC+AGG | - | chr7.3:2425090-2425109 | MS.gene22886:CDS | 45.0% | |
| CTCTCCTGTGTAATCGAATG+CGG | + | chr7.3:2424274-2424293 | None:intergenic | 45.0% | |
| CTTTGAACCACATGGACAGA+GGG | + | chr7.3:2425120-2425139 | None:intergenic | 45.0% | |
| GATGGTCGGTACAAATCCAA+TGG | - | chr7.3:2425046-2425065 | MS.gene22886:CDS | 45.0% | |
| TCTTTGAACCACATGGACAG+AGG | + | chr7.3:2425121-2425140 | None:intergenic | 45.0% | |
| !!! | TGTATTATTTATTTTTATAT+AGG | + | chr7.3:2424725-2424744 | None:intergenic | 5.0% |
| CGCATTCGATTACACAGGAG+AGG | - | chr7.3:2424272-2424291 | MS.gene22886:intron | 50.0% | |
| AGGAATGCCCTCTGTCCATG+TGG | - | chr7.3:2425110-2425129 | MS.gene22886:CDS | 55.0% | |
| CTCGCCGCATTCGATTACAC+AGG | - | chr7.3:2424267-2424286 | MS.gene22886:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 2424159 | 2425153 | 2424159 | ID=MS.gene22886 |
| chr7.3 | mRNA | 2424159 | 2425153 | 2424159 | ID=MS.gene22886.t1;Parent=MS.gene22886 |
| chr7.3 | exon | 2425019 | 2425153 | 2425019 | ID=MS.gene22886.t1.exon1;Parent=MS.gene22886.t1 |
| chr7.3 | CDS | 2425019 | 2425153 | 2425019 | ID=cds.MS.gene22886.t1;Parent=MS.gene22886.t1 |
| chr7.3 | exon | 2424785 | 2424946 | 2424785 | ID=MS.gene22886.t1.exon2;Parent=MS.gene22886.t1 |
| chr7.3 | CDS | 2424785 | 2424946 | 2424785 | ID=cds.MS.gene22886.t1;Parent=MS.gene22886.t1 |
| chr7.3 | exon | 2424159 | 2424274 | 2424159 | ID=MS.gene22886.t1.exon3;Parent=MS.gene22886.t1 |
| chr7.3 | CDS | 2424159 | 2424274 | 2424159 | ID=cds.MS.gene22886.t1;Parent=MS.gene22886.t1 |
| Gene Sequence |
| Protein sequence |
