Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23069.t1 | XP_013450258.1 | 97 | 101 | 3 | 0 | 1 | 101 | 1 | 101 | 4.40E-49 | 203.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23069.t1 | P27527 | 74.2 | 62 | 16 | 0 | 40 | 101 | 8 | 69 | 3.3e-21 | 102.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23069.t1 | A0A072U4K8 | 97.0 | 101 | 3 | 0 | 1 | 101 | 1 | 101 | 3.1e-49 | 203.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058223 | MS.gene23069 | 0.805362 | 1.38E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23069.t1 | MTR_7g106780 | 96.078 | 102 | 4 | 0 | 1 | 102 | 1 | 102 | 2.16e-70 | 206 |
| MS.gene23069.t1 | MTR_5g085270 | 80.392 | 102 | 19 | 1 | 1 | 102 | 1 | 101 | 4.91e-55 | 167 |
| MS.gene23069.t1 | MTR_8g466190 | 87.755 | 49 | 6 | 0 | 54 | 102 | 32 | 80 | 8.50e-27 | 95.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23069.t1 | AT5G47320 | 73.016 | 63 | 14 | 2 | 40 | 102 | 138 | 197 | 1.06e-24 | 93.2 |
Find 23 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGAGCTACAAACTTTCTT+AGG | 0.150952 | 7.2:-7334562 | MS.gene23069:CDS |
| ACATGAGAATCAACAAATTC+AGG | 0.356616 | 7.2:+7334350 | None:intergenic |
| TTACGCGCTGGCGCAGGAGG+AGG | 0.378631 | 7.2:+7334467 | None:intergenic |
| GGAAAAGTGGGTCATAAGTT+TGG | 0.399048 | 7.2:-7334281 | MS.gene23069:intron |
| CGCGCTGGCGCAGGAGGAGG+GGG | 0.407883 | 7.2:+7334470 | None:intergenic |
| GATGTATTTAGCTTACAATA+AGG | 0.427178 | 7.2:-7334075 | MS.gene23069:intron |
| TCCTGCGCCAGCGCGTAAAC+CGG | 0.432312 | 7.2:-7334462 | MS.gene23069:CDS |
| AATTTGTTGATTCTCATGTC+AGG | 0.439839 | 7.2:-7334346 | MS.gene23069:CDS |
| TAAAATCAGTGAGGGAAAAG+TGG | 0.445191 | 7.2:-7334294 | MS.gene23069:CDS |
| GAGAGCTACAAACTTTCTTA+GGG | 0.449124 | 7.2:-7334561 | MS.gene23069:CDS |
| TACGCGCTGGCGCAGGAGGA+GGG | 0.449303 | 7.2:+7334468 | None:intergenic |
| ACGCGCTGGCGCAGGAGGAG+GGG | 0.474195 | 7.2:+7334469 | None:intergenic |
| GCCGGTTTACGCGCTGGCGC+AGG | 0.480378 | 7.2:+7334461 | None:intergenic |
| GACAGCATTTGCAGTGTCGC+CGG | 0.519015 | 7.2:+7334511 | None:intergenic |
| AAACAGTTTCTCGGAATCGC+CGG | 0.545357 | 7.2:-7334530 | MS.gene23069:CDS |
| ATAAAAGCCGGTTTACGCGC+TGG | 0.551760 | 7.2:+7334455 | None:intergenic |
| AGAAATGCATCTATAAAAGC+CGG | 0.552909 | 7.2:+7334443 | None:intergenic |
| GGTTTACGCGCTGGCGCAGG+AGG | 0.553336 | 7.2:+7334464 | None:intergenic |
| AAAATCAGTGAGGGAAAAGT+GGG | 0.571193 | 7.2:-7334293 | MS.gene23069:CDS |
| ATTCGTTGTAAAATCAGTGA+GGG | 0.595357 | 7.2:-7334302 | MS.gene23069:CDS |
| AAAACAAACAAATTTGGTCA+AGG | 0.603956 | 7.2:-7334388 | MS.gene23069:CDS |
| TCTCATGTCAGGATTTACAA+TGG | 0.621399 | 7.2:-7334335 | MS.gene23069:CDS |
| CATTCGTTGTAAAATCAGTG+AGG | 0.678142 | 7.2:-7334303 | MS.gene23069:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAGGAAATTGATTTTAT+GGG | - | chr7.2:7334437-7334456 | MS.gene23069:CDS | 15.0% |
| !!! | AAAAAGAAGTGATTTCTTTT+AGG | - | chr7.2:7334421-7334440 | MS.gene23069:CDS | 20.0% |
| !!! | CTTTTAGGAAATTGATTTTA+TGG | - | chr7.2:7334436-7334455 | MS.gene23069:CDS | 20.0% |
| !!! | TTCTGAAAAACAAACAAATT+TGG | - | chr7.2:7334218-7334237 | MS.gene23069:intron | 20.0% |
| ! | AAAACAAACAAATTTGGTCA+AGG | - | chr7.2:7334224-7334243 | MS.gene23069:intron | 25.0% |
| ! | ATCAATGTGGATAAAGATTA+TGG | - | chr7.2:7334485-7334504 | MS.gene23069:CDS | 25.0% |
| !! | GATGTATTTAGCTTACAATA+AGG | - | chr7.2:7334537-7334556 | MS.gene23069:CDS | 25.0% |
| ACATGAGAATCAACAAATTC+AGG | + | chr7.2:7334265-7334284 | None:intergenic | 30.0% | |
| AGAAATGCATCTATAAAAGC+CGG | + | chr7.2:7334172-7334191 | None:intergenic | 30.0% | |
| AGTTGCTTAACTTTAGCATT+AGG | + | chr7.2:7334397-7334416 | None:intergenic | 30.0% | |
| ATTCGTTGTAAAATCAGTGA+GGG | - | chr7.2:7334310-7334329 | MS.gene23069:CDS | 30.0% | |
| TGCAGTTATTTACATCAATG+TGG | - | chr7.2:7334472-7334491 | MS.gene23069:CDS | 30.0% | |
| !! | AATTTGTTGATTCTCATGTC+AGG | - | chr7.2:7334266-7334285 | MS.gene23069:intron | 30.0% |
| !! | ACTGATTTTACAACGAATGA+AGG | + | chr7.2:7334308-7334327 | None:intergenic | 30.0% |
| !! | GTTTTGAACAAACAGTTTCT+CGG | - | chr7.2:7334073-7334092 | MS.gene23069:intron | 30.0% |
| !! | TGTTTGTTTTTCAGAAGTTC+AGG | + | chr7.2:7334214-7334233 | None:intergenic | 30.0% |
| AAAATCAGTGAGGGAAAAGT+GGG | - | chr7.2:7334319-7334338 | MS.gene23069:CDS | 35.0% | |
| CATTCGTTGTAAAATCAGTG+AGG | - | chr7.2:7334309-7334328 | MS.gene23069:CDS | 35.0% | |
| GAGAGCTACAAACTTTCTTA+GGG | - | chr7.2:7334051-7334070 | MS.gene23069:CDS | 35.0% | |
| TAAAATCAGTGAGGGAAAAG+TGG | - | chr7.2:7334318-7334337 | MS.gene23069:CDS | 35.0% | |
| TCTCATGTCAGGATTTACAA+TGG | - | chr7.2:7334277-7334296 | MS.gene23069:intron | 35.0% | |
| TGAGAGCTACAAACTTTCTT+AGG | - | chr7.2:7334050-7334069 | MS.gene23069:CDS | 35.0% | |
| ! | TTGGTGAGTTTGCTTTAACT+AGG | - | chr7.2:7334350-7334369 | MS.gene23069:CDS | 35.0% |
| GGAAAAGTGGGTCATAAGTT+TGG | - | chr7.2:7334331-7334350 | MS.gene23069:CDS | 40.0% | |
| AAACAGTTTCTCGGAATCGC+CGG | - | chr7.2:7334082-7334101 | MS.gene23069:intron | 45.0% | |
| ! | ATAAAAGCCGGTTTACGCGC+TGG | + | chr7.2:7334160-7334179 | None:intergenic | 50.0% |
| GACAGCATTTGCAGTGTCGC+CGG | + | chr7.2:7334104-7334123 | None:intergenic | 55.0% | |
| TCCTGCGCCAGCGCGTAAAC+CGG | - | chr7.2:7334150-7334169 | MS.gene23069:intron | 65.0% | |
| ! | GGTTTACGCGCTGGCGCAGG+AGG | + | chr7.2:7334151-7334170 | None:intergenic | 70.0% |
| ! | TACGCGCTGGCGCAGGAGGA+GGG | + | chr7.2:7334147-7334166 | None:intergenic | 70.0% |
| ! | TTACGCGCTGGCGCAGGAGG+AGG | + | chr7.2:7334148-7334167 | None:intergenic | 70.0% |
| ! | ACGCGCTGGCGCAGGAGGAG+GGG | + | chr7.2:7334146-7334165 | None:intergenic | 75.0% |
| ! | GCCGGTTTACGCGCTGGCGC+AGG | + | chr7.2:7334154-7334173 | None:intergenic | 75.0% |
| !! | CGCGCTGGCGCAGGAGGAGG+GGG | + | chr7.2:7334145-7334164 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 7334049 | 7334585 | 7334049 | ID=MS.gene23069 |
| chr7.2 | mRNA | 7334049 | 7334585 | 7334049 | ID=MS.gene23069.t1;Parent=MS.gene23069 |
| chr7.2 | exon | 7334282 | 7334585 | 7334282 | ID=MS.gene23069.t1.exon1;Parent=MS.gene23069.t1 |
| chr7.2 | CDS | 7334282 | 7334585 | 7334282 | ID=cds.MS.gene23069.t1;Parent=MS.gene23069.t1 |
| chr7.2 | exon | 7334049 | 7334086 | 7334049 | ID=MS.gene23069.t1.exon2;Parent=MS.gene23069.t1 |
| chr7.2 | CDS | 7334049 | 7334086 | 7334049 | ID=cds.MS.gene23069.t1;Parent=MS.gene23069.t1 |
| Gene Sequence |
| Protein sequence |
