AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058223


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058223.t1 MTR_2g034290 98.969 97 1 0 1 97 1 97 1.09e-66 195
MS.gene058223.t1 MTR_8g012945 94.737 95 5 0 1 95 1 95 2.35e-55 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058223.t1 AT5G47890 74.227 97 25 0 1 97 1 97 3.40e-53 161

Find 23 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGATCAATATGGGCAGTTT+TGG 0.205012 2.4:-56470253 None:intergenic
ACTGCATTCACGGATCAATA+TGG 0.266079 2.4:-56470263 None:intergenic
TACCATATCTTGCCCACAAT+TGG 0.333397 2.4:-56470297 None:intergenic
CCCCAATTGTGGGCAAGATA+TGG 0.341515 2.4:+56470295 MS.gene058223:CDS
AACCTTGCAGATGAGCTTGC+AGG 0.383491 2.4:-56470063 None:intergenic
ACCATATCTTGCCCACAATT+GGG 0.391447 2.4:-56470296 None:intergenic
TTTGAAGGCACTCGAAGATC+TGG 0.433173 2.4:+56471163 MS.gene058223:CDS
TAAGCAGTAATATCAAGCTT+TGG 0.441770 2.4:-56471201 None:intergenic
GTTCAACTCCACTGCATTCA+CGG 0.460928 2.4:-56470273 None:intergenic
CTAGATTTGGGTGTTGAGAA+AGG 0.466386 2.4:+56471107 MS.gene058223:intron
CTGCATTCACGGATCAATAT+GGG 0.474327 2.4:-56470262 None:intergenic
TGTTGAGAAAGGCATCAAAC+TGG 0.506700 2.4:+56471118 MS.gene058223:CDS
TGGAGTTGAACCCCAATTGT+GGG 0.534156 2.4:+56470285 MS.gene058223:CDS
ATGAGCTTGCAGGTGATGAC+TGG 0.535904 2.4:-56470053 None:intergenic
TGCTTTGGAAAGTAATGGCA+TGG 0.538620 2.4:+56469983 None:intergenic
CCATATCTTGCCCACAATTG+GGG 0.545870 2.4:-56470295 None:intergenic
CTCGAAGATCTGGTAAAAGC+AGG 0.549599 2.4:+56471173 MS.gene058223:CDS
GTGGAGTTGAACCCCAATTG+TGG 0.601433 2.4:+56470284 MS.gene058223:CDS
CACCTGCAAGCTCATCTGCA+AGG 0.602497 2.4:+56470061 MS.gene058223:CDS
GAGAAAGGCATCAAACTGGA+AGG 0.617748 2.4:+56471122 MS.gene058223:CDS
TCGAGTGCCTTCAAAATCTG+TGG 0.622764 2.4:-56471155 None:intergenic
ATATTGATCCGTGAATGCAG+TGG 0.663327 2.4:+56470265 MS.gene058223:CDS
TGGAAAGTAATGGCATGGAG+AGG 0.677012 2.4:+56469988 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTATTAATGATTAAATTAT+AGG - chr2.4:56471089-56471108 None:intergenic 10.0%
!! TAAATTCATTTCAATGTTAA+AGG + chr2.4:56470523-56470542 MS.gene058223:intron 15.0%
!! TAACATTGAAATGAATTTAA+GGG - chr2.4:56470522-56470541 None:intergenic 15.0%
!! TAATCATTAATACTAGATTT+GGG + chr2.4:56471095-56471114 MS.gene058223:intron 15.0%
!! TTAACATTGAAATGAATTTA+AGG - chr2.4:56470523-56470542 None:intergenic 15.0%
!! TTAATCATTAATACTAGATT+TGG + chr2.4:56471094-56471113 MS.gene058223:intron 15.0%
!!! AATTAATGCTATGAAATTTT+AGG - chr2.4:56470116-56470135 None:intergenic 15.0%
!!! TTTTGTTGAAAAGAATTATA+AGG + chr2.4:56470213-56470232 MS.gene058223:CDS 15.0%
!! AAAAAGAAAGAGATTTAAGA+TGG - chr2.4:56470327-56470346 None:intergenic 20.0%
!! ATACAAAAATTTACAGTATG+TGG - chr2.4:56470966-56470985 None:intergenic 20.0%
!! ATTATACACAACTTCTAATT+GGG - chr2.4:56470867-56470886 None:intergenic 20.0%
!! GTATTAATGAATGGAAAAAT+TGG + chr2.4:56470793-56470812 MS.gene058223:intron 20.0%
!! TGATTAAATTATAGGATGTT+AGG - chr2.4:56471081-56471100 None:intergenic 20.0%
!!! AAAAGAAAGAGATTTAAGAT+GGG - chr2.4:56470326-56470345 None:intergenic 20.0%
!!! AAATTTTAGGTTCAAAGATA+GGG - chr2.4:56470103-56470122 None:intergenic 20.0%
!!! TAATTTTCTTTATGCATTCA+AGG - chr2.4:56470941-56470960 None:intergenic 20.0%
!!! TACTATTTTTTCTTATCTCA+GGG + chr2.4:56470189-56470208 MS.gene058223:intron 20.0%
!!! TTACTATTTTTTCTTATCTC+AGG + chr2.4:56470188-56470207 MS.gene058223:intron 20.0%
! AGTTGTGTATAATGTCAATT+CGG + chr2.4:56470874-56470893 MS.gene058223:intron 25.0%
! ATCCTTCAAGTATTAATGAA+TGG + chr2.4:56470784-56470803 MS.gene058223:intron 25.0%
! CATTATACACAACTTCTAAT+TGG - chr2.4:56470868-56470887 None:intergenic 25.0%
! TATAATATTCTCATCTCTGA+AGG - chr2.4:56470669-56470688 None:intergenic 25.0%
! TTCCATTCATTAATACTTGA+AGG - chr2.4:56470789-56470808 None:intergenic 25.0%
!! AAAAGAATGCTTTGAGTTAT+TGG - chr2.4:56470549-56470568 None:intergenic 25.0%
!!! AATAACTCAAAGCATTCTTT+TGG + chr2.4:56470548-56470567 MS.gene058223:intron 25.0%
!!! AATTTTAGGTTCAAAGATAG+GGG - chr2.4:56470102-56470121 None:intergenic 25.0%
!!! GAAATTTTAGGTTCAAAGAT+AGG - chr2.4:56470104-56470123 None:intergenic 25.0%
!!! GAGATAAATTTGTTAGTATC+TGG + chr2.4:56470589-56470608 MS.gene058223:intron 25.0%
!!! TAAGATTATTTTGCGTATTG+TGG + chr2.4:56470911-56470930 MS.gene058223:intron 25.0%
ACAAAATAATGCAGATGAGA+AGG - chr2.4:56471039-56471058 None:intergenic 30.0%
ATGAATGGAAAAATTGGAGT+TGG + chr2.4:56470799-56470818 MS.gene058223:intron 30.0%
ATTTGTTTGTATGAATCGCA+AGG + chr2.4:56470753-56470772 MS.gene058223:intron 30.0%
GTATACTTACTCTTCAAAGA+TGG + chr2.4:56470982-56471001 MS.gene058223:intron 30.0%
TTAAGGAACTAAGGAGAATA+TGG - chr2.4:56470387-56470406 None:intergenic 30.0%
! CATCATGTATAGTGTTCTTT+TGG + chr2.4:56470616-56470635 MS.gene058223:intron 30.0%
! GTTTTTAACCAACCGATAAA+GGG - chr2.4:56470837-56470856 None:intergenic 30.0%
! TTTTTAACCAACCGATAAAG+GGG - chr2.4:56470836-56470855 None:intergenic 30.0%
!! ATTATTTTGCGTATTGTGGA+TGG + chr2.4:56470915-56470934 MS.gene058223:intron 30.0%
!! TGTTTTTAACCAACCGATAA+AGG - chr2.4:56470838-56470857 None:intergenic 30.0%
!! TTGGTGGTTAATTTGTTAGA+GGG + chr2.4:56470567-56470586 MS.gene058223:intron 30.0%
!! TTTGGTGGTTAATTTGTTAG+AGG + chr2.4:56470566-56470585 MS.gene058223:intron 30.0%
AAATCCCAGTTAAGGAACTA+AGG - chr2.4:56470396-56470415 None:intergenic 35.0%
AAGAACTTTCACCCCTTTAT+CGG + chr2.4:56470822-56470841 MS.gene058223:intron 35.0%
ATTCTCCTTAGTTCCTTAAC+TGG + chr2.4:56470388-56470407 MS.gene058223:intron 35.0%
TTCTCCTTAGTTCCTTAACT+GGG + chr2.4:56470389-56470408 MS.gene058223:intron 35.0%
!! AACTCAAAGCATTCTTTTGG+TGG + chr2.4:56470551-56470570 MS.gene058223:intron 35.0%
ACCATATCTTGCCCACAATT+GGG - chr2.4:56470299-56470318 None:intergenic 40.0%
ACTGCATTCACGGATCAATA+TGG - chr2.4:56470266-56470285 None:intergenic 40.0%
ATATTGATCCGTGAATGCAG+TGG + chr2.4:56470265-56470284 MS.gene058223:CDS 40.0%
CTGCATTCACGGATCAATAT+GGG - chr2.4:56470265-56470284 None:intergenic 40.0%
CTTCCTACAAATCCCAGTTA+AGG - chr2.4:56470404-56470423 None:intergenic 40.0%
GAATGTGAATGCAAATCACG+TGG - chr2.4:56470365-56470384 None:intergenic 40.0%
GTTCCTTAACTGGGATTTGT+AGG + chr2.4:56470398-56470417 MS.gene058223:intron 40.0%
TACCATATCTTGCCCACAAT+TGG - chr2.4:56470300-56470319 None:intergenic 40.0%
! GGATCAATATGGGCAGTTTT+GGG - chr2.4:56470255-56470274 None:intergenic 40.0%
!! CTAGATTTGGGTGTTGAGAA+AGG + chr2.4:56471107-56471126 MS.gene058223:intron 40.0%
!! TGTTGAGAAAGGCATCAAAC+TGG + chr2.4:56471118-56471137 MS.gene058223:CDS 40.0%
ACTTTCACCCCTTTATCGGT+TGG + chr2.4:56470826-56470845 MS.gene058223:intron 45.0%
CCATATCTTGCCCACAATTG+GGG - chr2.4:56470298-56470317 None:intergenic 45.0%
CTCGAAGATCTGGTAAAAGC+AGG + chr2.4:56471173-56471192 MS.gene058223:CDS 45.0%
GTTCAACTCCACTGCATTCA+CGG - chr2.4:56470276-56470295 None:intergenic 45.0%
TCGAGTGCCTTCAAAATCTG+TGG - chr2.4:56471158-56471177 None:intergenic 45.0%
TGGAGTTGAACCCCAATTGT+GGG + chr2.4:56470285-56470304 MS.gene058223:CDS 45.0%
! CGGATCAATATGGGCAGTTT+TGG - chr2.4:56470256-56470275 None:intergenic 45.0%
! GACAGAGCCACAGATTTTGA+AGG + chr2.4:56471148-56471167 MS.gene058223:CDS 45.0%
!! GAGAAAGGCATCAAACTGGA+AGG + chr2.4:56471122-56471141 MS.gene058223:CDS 45.0%
!! TTTGAAGGCACTCGAAGATC+TGG + chr2.4:56471163-56471182 MS.gene058223:CDS 45.0%
!!! ATAATATTTAAAATTAGTTT+TGG - chr2.4:56470707-56470726 None:intergenic 5.0%
AACCTTGCAGATGAGCTTGC+AGG - chr2.4:56470066-56470085 None:intergenic 50.0%
ATGAGCTTGCAGGTGATGAC+TGG - chr2.4:56470056-56470075 None:intergenic 50.0%
CCCCAATTGTGGGCAAGATA+TGG + chr2.4:56470295-56470314 MS.gene058223:CDS 50.0%
GTGGAGTTGAACCCCAATTG+TGG + chr2.4:56470284-56470303 MS.gene058223:CDS 50.0%
AAGGCGTAGCCTAGTGAGTC+GGG - chr2.4:56470650-56470669 None:intergenic 55.0%
CACCTGCAAGCTCATCTGCA+AGG + chr2.4:56470061-56470080 MS.gene058223:CDS 55.0%
GTAGCCTAGTGAGTCGGGTT+GGG - chr2.4:56470645-56470664 None:intergenic 55.0%
! GAAGGCGTAGCCTAGTGAGT+CGG - chr2.4:56470651-56470670 None:intergenic 55.0%
CGTAGCCTAGTGAGTCGGGT+TGG - chr2.4:56470646-56470665 None:intergenic 60.0%
GTGTCCCAACCCGACTCACT+AGG + chr2.4:56470638-56470657 MS.gene058223:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 56469997 56471211 56469997 ID=MS.gene058223
chr2.4 mRNA 56469997 56471211 56469997 ID=MS.gene058223.t1;Parent=MS.gene058223
chr2.4 exon 56469997 56470082 56469997 ID=MS.gene058223.t1.exon1;Parent=MS.gene058223.t1
chr2.4 CDS 56469997 56470082 56469997 ID=cds.MS.gene058223.t1;Parent=MS.gene058223.t1
chr2.4 exon 56470210 56470316 56470210 ID=MS.gene058223.t1.exon2;Parent=MS.gene058223.t1
chr2.4 CDS 56470210 56470316 56470210 ID=cds.MS.gene058223.t1;Parent=MS.gene058223.t1
chr2.4 exon 56471111 56471211 56471111 ID=MS.gene058223.t1.exon3;Parent=MS.gene058223.t1
chr2.4 CDS 56471111 56471211 56471111 ID=cds.MS.gene058223.t1;Parent=MS.gene058223.t1
Gene Sequence

>MS.gene058223

ATGGCATGGAGAGGACAACTTTCAAAGAACATCAAAGAGCTTCGATTTCTGATGTGCCAGTCATCACCTGCAAGCTCATCTGCAAGGTTATATATATTTTTTCCCCTATCTTTGAACCTAAAATTTCATAGCATTAATTTTTAAAAATCTTTTTATTAAATTTCGTACATACTTGAATCTTTAATGAGCTCTTACTATTTTTTCTTATCTCAGGGCTTTTGTTGAAAAGAATTATAAGGAACTAAAGACATTGAACCCAAAACTGCCCATATTGATCCGTGAATGCAGTGGAGTTGAACCCCAATTGTGGGCAAGATATGGTATCGCCCATCTTAAATCTCTTTCTTTTTACTTTGCATTTCATACCACGTGATTTGCATTCACATTCCATATTCTCCTTAGTTCCTTAACTGGGATTTGTAGGAAGTAAACATAATGCTTTTACACTGAAAATATGCTTCTTCTTCTTCTTTTTGTTTTTTTTTTTACAAGAACACTCAAAATATGATAATCAGCATTCTTCCCTTAAATTCATTTCAATGTTAAAGGCCAATAACTCAAAGCATTCTTTTGGTGGTTAATTTGTTAGAGGGAGATAAATTTGTTAGTATCTGGATCTCATCATGTATAGTGTTCTTTTGGTGTCCCAACCCGACTCACTAGGCTACGCCTTCAGAGATGAGAATATTATAGTATGAGTAGTCTGTCCAAAACTAATTTTAAATATTATATCAATTTTGACTTTTATCTATGAACATTTGTTTGTATGAATCGCAAGGAGTCTGATATCCTTCAAGTATTAATGAATGGAAAAATTGGAGTTGGAAGAACTTTCACCCCTTTATCGGTTGGTTAAAAACAACTTGACCCAATTAGAAGTTGTGTATAATGTCAATTCGGTTTTAATTTTTACTTAAGATTATTTTGCGTATTGTGGATGGCCTTGAATGCATAAAGAAAATTAAGCCACATACTGTAAATTTTTGTATACTTACTCTTCAAAGATGGTTCACATTTCTGATCATATCTTGTAGCTCATCCTTCTCATCTGCATTATTTTGTTTATCTATTTGTGATATATCCTAACATCCTATAATTTAATCATTAATACTAGATTTGGGTGTTGAGAAAGGCATCAAACTGGAAGGCATGACAGAGCCACAGATTTTGAAGGCACTCGAAGATCTGGTAAAAGCAGGACAATCCAAAGCTTGA

Protein sequence

>MS.gene058223.t1

MAWRGQLSKNIKELRFLMCQSSPASSSARAFVEKNYKELKTLNPKLPILIRECSGVEPQLWARYDLGVEKGIKLEGMTEPQILKALEDLVKAGQSKA