Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23079.t1 | XP_013450239.1 | 94.6 | 130 | 7 | 0 | 1 | 130 | 1 | 130 | 1.50E-61 | 245.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23079.t1 | Q9FM54 | 65.1 | 126 | 43 | 1 | 2 | 126 | 4 | 129 | 4.4e-38 | 158.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23079.t1 | A0A072U3F9 | 94.6 | 130 | 7 | 0 | 1 | 130 | 1 | 130 | 1.1e-61 | 245.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049887 | MS.gene23079 | 0.807877 | 4.08E-50 | -1.69E-46 |
| MS.gene051746 | MS.gene23079 | 0.805661 | 1.20E-49 | -1.69E-46 |
| MS.gene051850 | MS.gene23079 | 0.832621 | 8.51E-56 | -1.69E-46 |
| MS.gene051903 | MS.gene23079 | 0.800147 | 1.65E-48 | -1.69E-46 |
| MS.gene056075 | MS.gene23079 | 0.82355 | 1.30E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23079.t1 | MTR_7g106600 | 94.615 | 130 | 7 | 0 | 1 | 130 | 1 | 130 | 1.30e-86 | 248 |
| MS.gene23079.t1 | MTR_2g088720 | 85.271 | 129 | 19 | 0 | 1 | 129 | 4 | 132 | 2.28e-79 | 230 |
| MS.gene23079.t1 | MTR_2g088720 | 85.271 | 129 | 19 | 0 | 1 | 129 | 15 | 143 | 3.71e-79 | 230 |
| MS.gene23079.t1 | MTR_7g106580 | 82.946 | 129 | 22 | 0 | 2 | 130 | 5 | 133 | 2.22e-74 | 217 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23079.t1 | AT5G62980 | 65.079 | 126 | 43 | 1 | 2 | 126 | 4 | 129 | 8.12e-53 | 163 |
| MS.gene23079.t1 | AT5G62980 | 65.079 | 126 | 43 | 1 | 2 | 126 | 4 | 129 | 8.12e-53 | 163 |
| MS.gene23079.t1 | AT3G11750 | 61.983 | 121 | 45 | 1 | 7 | 126 | 21 | 141 | 2.58e-49 | 154 |
| MS.gene23079.t1 | AT3G21730 | 50.000 | 120 | 59 | 1 | 8 | 126 | 40 | 159 | 8.93e-39 | 128 |
| MS.gene23079.t1 | AT3G21730 | 43.750 | 64 | 35 | 1 | 64 | 126 | 12 | 75 | 4.17e-13 | 60.5 |
Find 26 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAGGGGATTGACATTCTT+TGG | 0.182580 | 7.2:-7411529 | MS.gene23079:CDS |
| AAAGCCTCATGTGGCTATTC+AGG | 0.228909 | 7.2:-7410486 | MS.gene23079:CDS |
| AGGACCAGCTCAAAACCTTC+TGG | 0.353642 | 7.2:-7410585 | MS.gene23079:CDS |
| TTAGCTACGCTGAAATATAT+CGG | 0.447993 | 7.2:-7411393 | MS.gene23079:intron |
| TCAACGCCTAAGTAATCAAC+CGG | 0.493700 | 7.2:+7410461 | None:intergenic |
| TCATGTGGCTATTCAGGGTC+CGG | 0.501646 | 7.2:-7410480 | MS.gene23079:CDS |
| AAGCCTCATGTGGCTATTCA+GGG | 0.505045 | 7.2:-7410485 | MS.gene23079:CDS |
| TGGATGGATCTTAAACCAGC+TGG | 0.524717 | 7.2:-7411442 | MS.gene23079:CDS |
| CAGGGTCCGGTTGATTACTT+AGG | 0.534205 | 7.2:-7410467 | MS.gene23079:CDS |
| CTACAACGAACTTCTGTCCA+AGG | 0.552732 | 7.2:+7411476 | None:intergenic |
| CTCTTTGTGAATTGTCAAAG+TGG | 0.557952 | 7.2:+7410535 | None:intergenic |
| GTGGAGACAAACTGATTCTG+AGG | 0.567883 | 7.2:-7411546 | MS.gene23079:CDS |
| TACAACGAACTTCTGTCCAA+GGG | 0.568116 | 7.2:+7411477 | None:intergenic |
| TCTGCTGTTCGTGTGAAGAT+CGG | 0.568597 | 7.2:-7410509 | MS.gene23079:CDS |
| ATAGCTAAGGAAGTTGTTGA+AGG | 0.569588 | 7.2:-7410605 | MS.gene23079:CDS |
| AAATGATCAGAAATTCCAGC+TGG | 0.574592 | 7.2:+7411427 | None:intergenic |
| AAAATGGATGCGTTAATAGG+TGG | 0.579943 | 7.2:-7411565 | MS.gene23079:CDS |
| TGTAGATATAGATGCTTGGA+TGG | 0.580239 | 7.2:-7411458 | MS.gene23079:CDS |
| TGGAGACAAACTGATTCTGA+GGG | 0.582302 | 7.2:-7411545 | MS.gene23079:CDS |
| TGGTTGATGCAGTATAGCTA+AGG | 0.588966 | 7.2:-7410618 | MS.gene23079:intron |
| TCGTTGTAGATATAGATGCT+TGG | 0.592315 | 7.2:-7411462 | MS.gene23079:CDS |
| TCAAAACCTTCTGGAATCAG+TGG | 0.595819 | 7.2:-7410576 | MS.gene23079:CDS |
| TTTGGGCCACTGATTCCAGA+AGG | 0.599019 | 7.2:+7410570 | None:intergenic |
| GAAGATCGGAAAGCCTCATG+TGG | 0.680483 | 7.2:-7410495 | MS.gene23079:CDS |
| GGACCCTGAATAGCCACATG+AGG | 0.705107 | 7.2:+7410482 | None:intergenic |
| GGAGACAAACTGATTCTGAG+GGG | 0.749444 | 7.2:-7411544 | MS.gene23079:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATTAAATTAAATAGTAG+TGG | + | chr7.2:7410780-7410799 | None:intergenic | 10.0% |
| !! | AAATACAATTCAAAATAACA+GGG | + | chr7.2:7411001-7411020 | None:intergenic | 15.0% |
| !! | TATACTGCTATAAATTATAA+TGG | - | chr7.2:7410941-7410960 | MS.gene23079:intron | 15.0% |
| !!! | AATCTCTGAAAAATAAATAA+CGG | - | chr7.2:7410860-7410879 | MS.gene23079:intron | 15.0% |
| !! | ATCTACAGTATAATTTGAAT+TGG | + | chr7.2:7410837-7410856 | None:intergenic | 20.0% |
| !! | CTAAAACAGAAGATAAAATA+GGG | + | chr7.2:7411237-7411256 | None:intergenic | 20.0% |
| !! | GAAATACAATTCAAAATAAC+AGG | + | chr7.2:7411002-7411021 | None:intergenic | 20.0% |
| !! | TCATAAGAATAATGATAAAG+TGG | + | chr7.2:7410635-7410654 | None:intergenic | 20.0% |
| !!! | TCAAAAATTACTCTTCTTTT+GGG | + | chr7.2:7411299-7411318 | None:intergenic | 20.0% |
| !!! | TCTAAAACAGAAGATAAAAT+AGG | + | chr7.2:7411238-7411257 | None:intergenic | 20.0% |
| ! | AAATTTAATATCATCAACCC+TGG | + | chr7.2:7410907-7410926 | None:intergenic | 25.0% |
| ! | CTATCATTGAGAGTTTATAT+GGG | + | chr7.2:7411174-7411193 | None:intergenic | 25.0% |
| ! | TAACATTCTGAACATACTTT+GGG | - | chr7.2:7411047-7411066 | MS.gene23079:intron | 25.0% |
| ! | TTAAACTAAACATACACACT+TGG | - | chr7.2:7411351-7411370 | MS.gene23079:intron | 25.0% |
| ! | TTAACATTCTGAACATACTT+TGG | - | chr7.2:7411046-7411065 | MS.gene23079:intron | 25.0% |
| !! | ATAAATAACGGAGTTGTTAA+TGG | - | chr7.2:7410872-7410891 | MS.gene23079:intron | 25.0% |
| !! | CAAAAGAAGAGTAATTTTTG+AGG | - | chr7.2:7411298-7411317 | MS.gene23079:intron | 25.0% |
| !! | CTCAAAAATTACTCTTCTTT+TGG | + | chr7.2:7411300-7411319 | None:intergenic | 25.0% |
| !! | TGAATAGTTTGTGCTTAATT+TGG | - | chr7.2:7411022-7411041 | MS.gene23079:intron | 25.0% |
| !!! | TTATGAGTGAGTATTGAAAA+TGG | - | chr7.2:7410731-7410750 | MS.gene23079:intron | 25.0% |
| ATAAAGGAAGAAAAAACCCT+TGG | - | chr7.2:7410496-7410515 | MS.gene23079:CDS | 30.0% | |
| GCTATCATTGAGAGTTTATA+TGG | + | chr7.2:7411175-7411194 | None:intergenic | 30.0% | |
| GTTTATATGGGAAATAAGCT+TGG | + | chr7.2:7411162-7411181 | None:intergenic | 30.0% | |
| TTAGCTACGCTGAAATATAT+CGG | - | chr7.2:7410596-7410615 | MS.gene23079:CDS | 30.0% | |
| ! | CAAGCATTGTGAAACTATTT+AGG | - | chr7.2:7411108-7411127 | MS.gene23079:intron | 30.0% |
| !!! | AAGTGGAGATTGCAATTTTT+TGG | + | chr7.2:7411440-7411459 | None:intergenic | 30.0% |
| !!! | AGTGGAGATTGCAATTTTTT+GGG | + | chr7.2:7411439-7411458 | None:intergenic | 30.0% |
| !!! | GAGTGAGTGTTGAATATTTT+TGG | - | chr7.2:7410704-7410723 | MS.gene23079:intron | 30.0% |
| !!! | TTGACATTCTTTGGTTTTCA+TGG | - | chr7.2:7410469-7410488 | MS.gene23079:CDS | 30.0% |
| AAATGATCAGAAATTCCAGC+TGG | + | chr7.2:7410565-7410584 | None:intergenic | 35.0% | |
| AAGCTCCACAAACATATGTT+TGG | + | chr7.2:7411324-7411343 | None:intergenic | 35.0% | |
| ATAGCTAAGGAAGTTGTTGA+AGG | - | chr7.2:7411384-7411403 | MS.gene23079:intron | 35.0% | |
| CTCTTTGTGAATTGTCAAAG+TGG | + | chr7.2:7411457-7411476 | None:intergenic | 35.0% | |
| GTTAATGGTAAATCTGTCCA+GGG | - | chr7.2:7410887-7410906 | MS.gene23079:intron | 35.0% | |
| TCACTCTGAGCATTAAGTTT+AGG | + | chr7.2:7410690-7410709 | None:intergenic | 35.0% | |
| TCGTTGTAGATATAGATGCT+TGG | - | chr7.2:7410527-7410546 | MS.gene23079:CDS | 35.0% | |
| TGTAGATATAGATGCTTGGA+TGG | - | chr7.2:7410531-7410550 | MS.gene23079:CDS | 35.0% | |
| TGTTAATGGTAAATCTGTCC+AGG | - | chr7.2:7410886-7410905 | MS.gene23079:intron | 35.0% | |
| ! | GAACATACTTTGGGTTTGTT+TGG | - | chr7.2:7411056-7411075 | MS.gene23079:intron | 35.0% |
| ! | TGGTTTTCATGGAGCAATAA+AGG | - | chr7.2:7410480-7410499 | MS.gene23079:CDS | 35.0% |
| !! | TACTCTTCTTTTGGGAACAA+AGG | + | chr7.2:7411291-7411310 | None:intergenic | 35.0% |
| TACAACGAACTTCTGTCCAA+GGG | + | chr7.2:7410515-7410534 | None:intergenic | 40.0% | |
| TCAACGCCTAAGTAATCAAC+CGG | + | chr7.2:7411531-7411550 | None:intergenic | 40.0% | |
| TGAGGCCAAACATATGTTTG+TGG | - | chr7.2:7411316-7411335 | MS.gene23079:intron | 40.0% | |
| ! | TGGAGACAAACTGATTCTGA+GGG | - | chr7.2:7410444-7410463 | MS.gene23079:CDS | 40.0% |
| ! | TGGTTGATGCAGTATAGCTA+AGG | - | chr7.2:7411371-7411390 | MS.gene23079:intron | 40.0% |
| !! | TCAAAACCTTCTGGAATCAG+TGG | - | chr7.2:7411413-7411432 | MS.gene23079:CDS | 40.0% |
| AAAGCCTCATGTGGCTATTC+AGG | - | chr7.2:7411503-7411522 | MS.gene23079:CDS | 45.0% | |
| AAGCCTCATGTGGCTATTCA+GGG | - | chr7.2:7411504-7411523 | MS.gene23079:CDS | 45.0% | |
| CTACAACGAACTTCTGTCCA+AGG | + | chr7.2:7410516-7410535 | None:intergenic | 45.0% | |
| CTGAGGGGATTGACATTCTT+TGG | - | chr7.2:7410460-7410479 | MS.gene23079:CDS | 45.0% | |
| GGCAGTACCAATGTTAGACT+CGG | + | chr7.2:7410980-7410999 | None:intergenic | 45.0% | |
| TCTGCTGTTCGTGTGAAGAT+CGG | - | chr7.2:7411480-7411499 | MS.gene23079:CDS | 45.0% | |
| TGGATGGATCTTAAACCAGC+TGG | - | chr7.2:7410547-7410566 | MS.gene23079:CDS | 45.0% | |
| ! | GGAGACAAACTGATTCTGAG+GGG | - | chr7.2:7410445-7410464 | MS.gene23079:CDS | 45.0% |
| ! | GTGGAGACAAACTGATTCTG+AGG | - | chr7.2:7410443-7410462 | MS.gene23079:CDS | 45.0% |
| !!! | GATTCCAGAAGGTTTTGAGC+TGG | + | chr7.2:7411411-7411430 | None:intergenic | 45.0% |
| AGGACCAGCTCAAAACCTTC+TGG | - | chr7.2:7411404-7411423 | MS.gene23079:CDS | 50.0% | |
| CAGGGTCCGGTTGATTACTT+AGG | - | chr7.2:7411522-7411541 | MS.gene23079:CDS | 50.0% | |
| CCACGTACCGAGTCTAACAT+TGG | - | chr7.2:7410970-7410989 | MS.gene23079:intron | 50.0% | |
| GAAGATCGGAAAGCCTCATG+TGG | - | chr7.2:7411494-7411513 | MS.gene23079:CDS | 50.0% | |
| TCATGTGGCTATTCAGGGTC+CGG | - | chr7.2:7411509-7411528 | MS.gene23079:CDS | 50.0% | |
| ! | CCAATGTTAGACTCGGTACG+TGG | + | chr7.2:7410973-7410992 | None:intergenic | 50.0% |
| !! | TTTGGGCCACTGATTCCAGA+AGG | + | chr7.2:7411422-7411441 | None:intergenic | 50.0% |
| GGACCCTGAATAGCCACATG+AGG | + | chr7.2:7411510-7411529 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 7410427 | 7411584 | 7410427 | ID=MS.gene23079 |
| chr7.2 | mRNA | 7410427 | 7411584 | 7410427 | ID=MS.gene23079.t1;Parent=MS.gene23079 |
| chr7.2 | exon | 7411394 | 7411584 | 7411394 | ID=MS.gene23079.t1.exon1;Parent=MS.gene23079.t1 |
| chr7.2 | CDS | 7411394 | 7411584 | 7411394 | ID=cds.MS.gene23079.t1;Parent=MS.gene23079.t1 |
| chr7.2 | exon | 7410427 | 7410628 | 7410427 | ID=MS.gene23079.t1.exon2;Parent=MS.gene23079.t1 |
| chr7.2 | CDS | 7410427 | 7410628 | 7410427 | ID=cds.MS.gene23079.t1;Parent=MS.gene23079.t1 |
| Gene Sequence |
| Protein sequence |
