AlfalfaGEDB | MS.gene23267

M. sativa cultivar XinJiangDaYe / MS.gene23267

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23267.t1	XP_003606627.1	97.3	148	4	0	1	148	1	148	3.80E-45	191

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23267.t1	O22582	92.8	111	8	0	38	148	37	147	3.0e-46	186.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23267.t1	G7JTQ7	97.3	148	4	0	1	148	1	148	2.8e-45	191.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051263	MS.gene23267	0.811857	5.68E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23267.t1	MTR_4g063220	99.324	148	1	0	1	148	1	148	3.84e-102	289
MS.gene23267.t1	MTR_4g063990	99.324	148	1	0	1	148	1	148	3.84e-102	289
MS.gene23267.t1	MTR_4g063200	99.324	148	1	0	1	148	1	148	3.84e-102	289
MS.gene23267.t1	MTR_4g064020	99.324	148	1	0	1	148	1	148	3.84e-102	289
MS.gene23267.t1	MTR_4g071180	99.324	148	1	0	1	148	1	148	8.19e-102	288
MS.gene23267.t1	MTR_4g070240	99.324	148	1	0	1	148	1	148	8.19e-102	288
MS.gene23267.t1	MTR_4g070245	97.973	148	3	0	1	148	1	148	4.03e-100	284
MS.gene23267.t1	MTR_4g063240	98.649	148	1	1	1	148	1	147	1.11e-99	283
MS.gene23267.t1	MTR_4g063275	96.622	148	5	0	1	148	1	148	8.15e-99	280
MS.gene23267.t1	MTR_4g064983	98.611	144	2	0	1	144	1	144	7.90e-97	279
MS.gene23267.t1	MTR_4g088025	93.919	148	4	1	1	148	1	143	7.97e-89	255
MS.gene23267.t1	MTR_1g090780	88.514	148	6	3	1	148	1	137	1.62e-78	229
MS.gene23267.t1	MTR_1g492910	87.838	148	7	3	1	148	1	137	8.01e-78	227
MS.gene23267.t1	MTR_1g068600	92.793	111	4	1	38	148	30	136	3.27e-64	192
MS.gene23267.t1	MTR_7g099960	92.793	111	5	1	38	148	31	138	6.03e-64	192
MS.gene23267.t1	MTR_2g084480	61.798	89	34	0	59	147	136	224	4.66e-37	127
MS.gene23267.t1	MTR_8g069990	68.817	93	21	3	15	107	4	88	3.43e-34	115
MS.gene23267.t1	MTR_4g063270	90.196	51	5	0	86	136	1	51	5.07e-27	95.9
MS.gene23267.t1	MTR_7g114580	90.244	41	4	0	108	148	11	51	9.18e-20	77.4
MS.gene23267.t1	MTR_4g032777	36.792	106	63	2	31	132	5	110	1.69e-13	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23267.t1	AT3G45980	88.742	151	13	2	1	148	1	150	1.41e-83	242
MS.gene23267.t1	AT2G28720	86.093	151	18	1	1	148	1	151	9.31e-81	235
MS.gene23267.t1	AT1G07790	86.928	153	10	3	1	148	1	148	7.39e-80	233
MS.gene23267.t1	AT5G59910	91.176	136	9	2	13	148	18	150	2.55e-77	226
MS.gene23267.t1	AT3G46030	94.444	126	6	1	23	148	21	145	8.32e-76	222
MS.gene23267.t1	AT2G37470	82.432	148	15	3	1	147	1	138	5.77e-71	210
MS.gene23267.t1	AT3G53650	81.208	149	16	3	1	148	1	138	4.43e-69	205
MS.gene23267.t1	AT5G22880	87.586	145	16	2	4	148	3	145	4.09e-67	200
MS.gene23267.t1	AT5G02570	79.730	148	14	1	1	148	1	132	9.33e-67	199
MS.gene23267.t1	AT3G09480	84.127	126	16	1	23	148	5	126	2.05e-62	187
MS.gene23267.t1	AT1G08170	61.176	85	33	0	61	145	151	235	2.87e-37	127

Find 46 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCTAAGAGCTTGTGAATT+TGG	0.203095	4.3:-76524062	None:intergenic
GGTAGCTTCTTTCCAGCCTT+TGG	0.299167	4.3:-76523728	None:intergenic
GCTTGTTCTGCCCGGTGAAT+TGG	0.304089	4.3:+76524003	MS.gene23267:CDS
GCTGGCTTCTTCTCTCCCTT+TGG	0.362568	4.3:-76523641	None:intergenic
CTTGTTGTACCTAGCGAGTC+TGG	0.384866	4.3:-76523939	None:intergenic
TCTGCGGGTTTCTTCTCTGC+TGG	0.399254	4.3:-76523659	None:intergenic
GCCTTTGGCTTCTTCTCCGC+CGG	0.402654	4.3:-76523713	None:intergenic
GCGGCTGAACCACCTTCCTT+TGG	0.410543	4.3:-76523749	None:intergenic
GTGGATTTCTTCTCCTCTGC+GGG	0.431011	4.3:-76523674	None:intergenic
TCTCTTCTTCTTCTTGTCTC+CGG	0.444808	4.3:-76523771	None:intergenic
ACCCTCAGAAACAGCATGCT+TGG	0.462043	4.3:-76524026	None:intergenic
TTGCTTGAGATACCAATGTC+CGG	0.465790	4.3:-76523854	None:intergenic
AGCATGCTTGGCCAATTCAC+CGG	0.471051	4.3:-76524014	None:intergenic
GGGAAATTCAGACCGCGGTC+AGG	0.471115	4.3:+76523981	MS.gene23267:CDS
TGTGAATTTGGTCACAGCCT+TGG	0.493306	4.3:-76524050	None:intergenic
AATTCCTTCAATGGCGCCAA+AGG	0.495983	4.3:+76523625	None:intergenic
GCGGAGAAGAAGCCAAAGGC+TGG	0.504484	4.3:+76523716	MS.gene23267:CDS
AAGGAAGGTGGTTCAGCCGC+CGG	0.504946	4.3:+76523752	MS.gene23267:CDS
TGCTTGAGATACCAATGTCC+GGG	0.518516	4.3:-76523853	None:intergenic
GCATGCTTGGCCAATTCACC+GGG	0.525255	4.3:-76524013	None:intergenic
TGGTATCTCAAGCAAAGCCA+TGG	0.533917	4.3:+76523862	MS.gene23267:CDS
GCCAAGCATGCTGTTTCTGA+GGG	0.538001	4.3:+76524025	MS.gene23267:CDS
GGCCAAGCATGCTGTTTCTG+AGG	0.538906	4.3:+76524024	MS.gene23267:CDS
CACAGTCGCTGAGAAAGCTC+CGG	0.552253	4.3:+76523694	MS.gene23267:CDS
GCGGTCAGGCTTGTTCTGCC+CGG	0.555879	4.3:+76523995	MS.gene23267:CDS
ATTCCTTCAATGGCGCCAAA+GGG	0.555903	4.3:+76523626	None:intergenic
ATTTCCCTCGAAGTGATCGT+CGG	0.569915	4.3:-76523965	None:intergenic
TGCTGTTTCTGAGGGTACCA+AGG	0.572597	4.3:+76524033	MS.gene23267:CDS
CTTCTTCTTCTTGTCTCCGG+CGG	0.574610	4.3:-76523768	None:intergenic
AAACAAGTTCACCCGGACAT+TGG	0.574952	4.3:+76523842	MS.gene23267:CDS
AGAAGCCGACGATCACTTCG+AGG	0.586465	4.3:+76523960	MS.gene23267:CDS
AAGAATCTTCCAGACTCGCT+AGG	0.594905	4.3:+76523930	MS.gene23267:CDS
TGTGGATTTCTTCTCCTCTG+CGG	0.602479	4.3:-76523675	None:intergenic
GGAAAGAAGCTACCAAAGGA+AGG	0.624050	4.3:+76523737	MS.gene23267:CDS
CGGAGCTTTCTCAGCGACTG+TGG	0.628186	4.3:-76523693	None:intergenic
GGCTGGAAAGAAGCTACCAA+AGG	0.634160	4.3:+76523733	MS.gene23267:CDS
AGTTCTGAAACAAGTTCACC+CGG	0.635033	4.3:+76523835	MS.gene23267:CDS
AGTCGCTGAGAAAGCTCCGG+CGG	0.656886	4.3:+76523697	MS.gene23267:CDS
GGTATCTCAAGCAAAGCCAT+GGG	0.659631	4.3:+76523863	MS.gene23267:CDS
GAAGCCGACGATCACTTCGA+GGG	0.660226	4.3:+76523961	MS.gene23267:CDS
AAGAAGCTACCAAAGGAAGG+TGG	0.666846	4.3:+76523740	MS.gene23267:CDS
TCCGGCGGAGAAGAAGCCAA+AGG	0.682003	4.3:+76523712	MS.gene23267:CDS
GAAACTGTTCATGATACCCA+TGG	0.683953	4.3:-76523879	None:intergenic
GGGCAGAACAAGCCTGACCG+CGG	0.695125	4.3:-76523993	None:intergenic
AGCAGAGAAGAAACCCGCAG+AGG	0.698236	4.3:+76523661	MS.gene23267:CDS
TTCGAGGGAAATTCAGACCG+CGG	0.709650	4.3:+76523976	MS.gene23267:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GAAACTGTTCATGATACCCA+TGG	-	chr4.3:76523882-76523901	None:intergenic	40.0%
	TCTCTTCTTCTTCTTGTCTC+CGG	-	chr4.3:76523774-76523793	None:intergenic	40.0%
	TTGCTTGAGATACCAATGTC+CGG	-	chr4.3:76523857-76523876	None:intergenic	40.0%
!!	AGTTCTGAAACAAGTTCACC+CGG	+	chr4.3:76523835-76523854	MS.gene23267:CDS	40.0%
	AAACAAGTTCACCCGGACAT+TGG	+	chr4.3:76523842-76523861	MS.gene23267:CDS	45.0%
	AAGAAGCTACCAAAGGAAGG+TGG	+	chr4.3:76523740-76523759	MS.gene23267:CDS	45.0%
	AAGAATCTTCCAGACTCGCT+AGG	+	chr4.3:76523930-76523949	MS.gene23267:CDS	45.0%
	ATTTCCCTCGAAGTGATCGT+CGG	-	chr4.3:76523968-76523987	None:intergenic	45.0%
	GGAAAGAAGCTACCAAAGGA+AGG	+	chr4.3:76523737-76523756	MS.gene23267:CDS	45.0%
	GGTATCTCAAGCAAAGCCAT+GGG	+	chr4.3:76523863-76523882	MS.gene23267:CDS	45.0%
	TGCTTGAGATACCAATGTCC+GGG	-	chr4.3:76523856-76523875	None:intergenic	45.0%
	TGGTATCTCAAGCAAAGCCA+TGG	+	chr4.3:76523862-76523881	MS.gene23267:CDS	45.0%
	TGTGAATTTGGTCACAGCCT+TGG	-	chr4.3:76524053-76524072	None:intergenic	45.0%
	TGTGGATTTCTTCTCCTCTG+CGG	-	chr4.3:76523678-76523697	None:intergenic	45.0%
	ACCCTCAGAAACAGCATGCT+TGG	-	chr4.3:76524029-76524048	None:intergenic	50.0%
	AGCATGCTTGGCCAATTCAC+CGG	-	chr4.3:76524017-76524036	None:intergenic	50.0%
	CTTCTTCTTCTTGTCTCCGG+CGG	-	chr4.3:76523771-76523790	None:intergenic	50.0%
	GGCTGGAAAGAAGCTACCAA+AGG	+	chr4.3:76523733-76523752	MS.gene23267:CDS	50.0%
	GGTAGCTTCTTTCCAGCCTT+TGG	-	chr4.3:76523731-76523750	None:intergenic	50.0%
	GTGGATTTCTTCTCCTCTGC+GGG	-	chr4.3:76523677-76523696	None:intergenic	50.0%
	TTCGAGGGAAATTCAGACCG+CGG	+	chr4.3:76523976-76523995	MS.gene23267:CDS	50.0%
!	CTTGTTGTACCTAGCGAGTC+TGG	-	chr4.3:76523942-76523961	None:intergenic	50.0%
!!	GCCAAGCATGCTGTTTCTGA+GGG	+	chr4.3:76524025-76524044	MS.gene23267:CDS	50.0%
!!	TGCTGTTTCTGAGGGTACCA+AGG	+	chr4.3:76524033-76524052	MS.gene23267:CDS	50.0%
	AGAAGCCGACGATCACTTCG+AGG	+	chr4.3:76523960-76523979	MS.gene23267:CDS	55.0%
	AGCAGAGAAGAAACCCGCAG+AGG	+	chr4.3:76523661-76523680	MS.gene23267:CDS	55.0%
	CACAGTCGCTGAGAAAGCTC+CGG	+	chr4.3:76523694-76523713	MS.gene23267:CDS	55.0%
	GAAGCCGACGATCACTTCGA+GGG	+	chr4.3:76523961-76523980	MS.gene23267:CDS	55.0%
	GCATGCTTGGCCAATTCACC+GGG	-	chr4.3:76524016-76524035	None:intergenic	55.0%
	GCTTGTTCTGCCCGGTGAAT+TGG	+	chr4.3:76524003-76524022	MS.gene23267:CDS	55.0%
	TCTGCGGGTTTCTTCTCTGC+TGG	-	chr4.3:76523662-76523681	None:intergenic	55.0%
!	GCTGGCTTCTTCTCTCCCTT+TGG	-	chr4.3:76523644-76523663	None:intergenic	55.0%
!!	GGCCAAGCATGCTGTTTCTG+AGG	+	chr4.3:76524024-76524043	MS.gene23267:CDS	55.0%
	AAGGAAGGTGGTTCAGCCGC+CGG	+	chr4.3:76523752-76523771	MS.gene23267:CDS	60.0%
	AGTCGCTGAGAAAGCTCCGG+CGG	+	chr4.3:76523697-76523716	MS.gene23267:CDS	60.0%
	CGGAGCTTTCTCAGCGACTG+TGG	-	chr4.3:76523696-76523715	None:intergenic	60.0%
	GCGGAGAAGAAGCCAAAGGC+TGG	+	chr4.3:76523716-76523735	MS.gene23267:CDS	60.0%
	GCGGCTGAACCACCTTCCTT+TGG	-	chr4.3:76523752-76523771	None:intergenic	60.0%
	GGGAAATTCAGACCGCGGTC+AGG	+	chr4.3:76523981-76524000	MS.gene23267:CDS	60.0%
	TCCGGCGGAGAAGAAGCCAA+AGG	+	chr4.3:76523712-76523731	MS.gene23267:CDS	60.0%
!	GCCTTTGGCTTCTTCTCCGC+CGG	-	chr4.3:76523716-76523735	None:intergenic	60.0%
	GGGCAGAACAAGCCTGACCG+CGG	-	chr4.3:76523996-76524015	None:intergenic	65.0%
!	GCGGTCAGGCTTGTTCTGCC+CGG	+	chr4.3:76523995-76524014	MS.gene23267:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	76523635	76524081	76523635	ID=MS.gene23267
chr4.3	mRNA	76523635	76524081	76523635	ID=MS.gene23267.t1;Parent=MS.gene23267
chr4.3	exon	76523635	76524081	76523635	ID=MS.gene23267.t1.exon1;Parent=MS.gene23267.t1
chr4.3	CDS	76523635	76524081	76523635	ID=cds.MS.gene23267.t1;Parent=MS.gene23267.t1

Gene Sequence

>MS.gene23267

ATGGCGCCAAAGGGAGAGAAGAAGCCAGCAGAGAAGAAACCCGCAGAGGAGAAGAAATCCACAGTCGCTGAGAAAGCTCCGGCGGAGAAGAAGCCAAAGGCTGGAAAGAAGCTACCAAAGGAAGGTGGTTCAGCCGCCGGAGACAAGAAGAAGAAGAGAAGCAAGAAGAGCGTTGAAACATACAAGATTTACATCTTCAAAGTTCTGAAACAAGTTCACCCGGACATTGGTATCTCAAGCAAAGCCATGGGTATCATGAACAGTTTCATCAACGACATCTTCGAGAAGCTCGCTCAAGAATCTTCCAGACTCGCTAGGTACAACAAGAAGCCGACGATCACTTCGAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGTGAATTGGCCAAGCATGCTGTTTCTGAGGGTACCAAGGCTGTGACCAAATTCACAAGCTCTTAG

Protein sequence

>MS.gene23267.t1

MAPKGEKKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS