AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051263


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051263.t1 MTR_7g052690 97.576 165 4 0 1 165 1 165 5.83e-113 318
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051263.t1 AT5G48480 54.135 133 57 3 21 152 23 152 5.35e-35 120

Find 37 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCAACAGCTTTCTTTGC+AGG 0.304196 7.3:-51034838 None:intergenic
CACAACAGCGTCGTTAGCTT+TGG 0.335053 7.3:-51033918 None:intergenic
AACAACGGGAGAAGCGGTTT+CGG 0.346996 7.3:-51033855 None:intergenic
CTTGATCAGCCTTACGCTTA+GGG 0.352790 7.3:-51033988 None:intergenic
TGTGAAGGCGCGTGTGGTGG+TGG 0.387525 7.3:+51034763 MS.gene051263:CDS
TCTTGATCAGCCTTACGCTT+AGG 0.390293 7.3:-51033989 None:intergenic
ACAACAGCGTCGTTAGCTTT+GGG 0.405498 7.3:-51033917 None:intergenic
TGGTGGTGGGCGCGTGGGAA+AGG 0.418463 7.3:+51034777 MS.gene051263:CDS
CTGATCCATACGGTTACGTT+TGG 0.429548 7.3:+51034803 MS.gene051263:CDS
CGCGTGTGGTGGTGGGCGCG+TGG 0.471387 7.3:+51034771 MS.gene051263:CDS
ATAGCAAAGGCAGTGAGTGC+TGG 0.474235 7.3:+51034712 MS.gene051263:CDS
GGGAGAAGCGGTTTCGGAAA+CGG 0.476505 7.3:-51033849 None:intergenic
AAAGCGACGAAGGAAACAAC+GGG 0.492629 7.3:-51033869 None:intergenic
GTGAAGGCGCGTGTGGTGGT+GGG 0.530940 7.3:+51034764 MS.gene051263:CDS
GCGTGTGGTGGTGGGCGCGT+GGG 0.533888 7.3:+51034772 MS.gene051263:CDS
TGCGCTGAACCCTAAGCGTA+AGG 0.549576 7.3:+51033979 MS.gene051263:CDS
AGTTCAGCAGAGAGAACAAG+AGG 0.563515 7.3:-51034016 None:intergenic
TCTGCTGAACTCAAGATCGC+TGG 0.565708 7.3:+51034028 MS.gene051263:CDS
TGGAACAGTGTGAAGAGTGG+TGG 0.570663 7.3:+51034643 MS.gene051263:intron
GCTTCCGAAACTCCTCAAAA+CGG 0.583220 7.3:+51033824 MS.gene051263:CDS
GAGTGTGAAGGCGCGTGTGG+TGG 0.585790 7.3:+51034760 MS.gene051263:CDS
AATTGCCAAACGTAACCGTA+TGG 0.585974 7.3:-51034808 None:intergenic
AGTGTGAAGAGTGGTGGAAA+CGG 0.593792 7.3:+51034649 MS.gene051263:intron
GGAGCTTCAACGAAGAGCTG+AGG 0.594711 7.3:-51033896 None:intergenic
CATCAACAGCATCAGCAACA+AGG 0.596823 7.3:-51034060 None:intergenic
CTGAGGCTTGAAAGCGACGA+AGG 0.599489 7.3:-51033879 None:intergenic
AACAGCATCAGCAACAAGGA+AGG 0.600915 7.3:-51034056 None:intergenic
CATTGAAGCTGCGATAGCAA+AGG 0.613057 7.3:+51034699 MS.gene051263:CDS
GAAGGAAACAACGGGAGAAG+CGG 0.617920 7.3:-51033861 None:intergenic
ACTCCTGCAAAGAAAGCTGT+TGG 0.629499 7.3:+51034835 MS.gene051263:CDS
GCTGAGTGTGAAGGCGCGTG+TGG 0.631571 7.3:+51034757 MS.gene051263:CDS
GGAAAGGTGACTGATCCATA+CGG 0.651721 7.3:+51034793 MS.gene051263:CDS
GAAAGCTGTTGGTGACGTGG+AGG 0.652063 7.3:+51034846 MS.gene051263:CDS
GAAAGCGACGAAGGAAACAA+CGG 0.664481 7.3:-51033870 None:intergenic
GGTGAAGTAGCTGAGTGTGA+AGG 0.666417 7.3:+51034748 MS.gene051263:CDS
AAAGAAAGCTGTTGGTGACG+TGG 0.670793 7.3:+51034843 MS.gene051263:CDS
AGTGCTGGAGCAGTTGCTGA+AGG 0.674380 7.3:+51034727 MS.gene051263:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTTTTTTATATTATTT+TGG + chr7.3:51034329-51034348 MS.gene051263:intron 0.0%
!!! TTTTTTTTTATAATTATTTT+TGG + chr7.3:51034623-51034642 MS.gene051263:intron 0.0%
!! AAAAACAAAATCAAAGATTA+CGG - chr7.3:51034453-51034472 None:intergenic 15.0%
!! AAAACAGTAAAATAAAAGTA+AGG - chr7.3:51034480-51034499 None:intergenic 15.0%
!! AAAAACTGTAGCATAATATA+AGG - chr7.3:51034414-51034433 None:intergenic 20.0%
!! CAAATGTAATGAAATAACTA+CGG - chr7.3:51034252-51034271 None:intergenic 20.0%
! GTTATGTCATGTTTATAGTT+AGG + chr7.3:51034295-51034314 MS.gene051263:intron 25.0%
!! TTTCAGATCTAGAACAAAAA+AGG - chr7.3:51034125-51034144 None:intergenic 25.0%
!!! CTTTGATCTGTTTTTAGATA+AGG + chr7.3:51034372-51034391 MS.gene051263:intron 25.0%
CAAAACGAAAATCACGACTA+TGG - chr7.3:51034216-51034235 None:intergenic 35.0%
! GAAAGAAACTCACGTTTTAG+CGG - chr7.3:51034089-51034108 None:intergenic 35.0%
AATTGCCAAACGTAACCGTA+TGG - chr7.3:51034811-51034830 None:intergenic 40.0%
!!! TTTTGGAACAGTGTGAAGAG+TGG + chr7.3:51034640-51034659 MS.gene051263:intron 40.0%
AAAGCGACGAAGGAAACAAC+GGG - chr7.3:51033872-51033891 None:intergenic 45.0%
AACAGCATCAGCAACAAGGA+AGG - chr7.3:51034059-51034078 None:intergenic 45.0%
ACTCCTGCAAAGAAAGCTGT+TGG + chr7.3:51034835-51034854 MS.gene051263:CDS 45.0%
AGTTCAGCAGAGAGAACAAG+AGG - chr7.3:51034019-51034038 None:intergenic 45.0%
CATCAACAGCATCAGCAACA+AGG - chr7.3:51034063-51034082 None:intergenic 45.0%
CATTGAAGCTGCGATAGCAA+AGG + chr7.3:51034699-51034718 MS.gene051263:CDS 45.0%
CTGATCCATACGGTTACGTT+TGG + chr7.3:51034803-51034822 MS.gene051263:CDS 45.0%
CTTGATCAGCCTTACGCTTA+GGG - chr7.3:51033991-51034010 None:intergenic 45.0%
GAAAGCGACGAAGGAAACAA+CGG - chr7.3:51033873-51033892 None:intergenic 45.0%
GCTTCCGAAACTCCTCAAAA+CGG + chr7.3:51033824-51033843 MS.gene051263:CDS 45.0%
GGAAAGGTGACTGATCCATA+CGG + chr7.3:51034793-51034812 MS.gene051263:CDS 45.0%
TCACCAACAGCTTTCTTTGC+AGG - chr7.3:51034841-51034860 None:intergenic 45.0%
TCTTGATCAGCCTTACGCTT+AGG - chr7.3:51033992-51034011 None:intergenic 45.0%
! AGTGTGAAGAGTGGTGGAAA+CGG + chr7.3:51034649-51034668 MS.gene051263:intron 45.0%
!! AAAGAAAGCTGTTGGTGACG+TGG + chr7.3:51034843-51034862 MS.gene051263:CDS 45.0%
!! ACAACAGCGTCGTTAGCTTT+GGG - chr7.3:51033920-51033939 None:intergenic 45.0%
!! AAAAAAATGAATAAATATTA+AGG - chr7.3:51034609-51034628 None:intergenic 5.0%
AACAACGGGAGAAGCGGTTT+CGG - chr7.3:51033858-51033877 None:intergenic 50.0%
ATAGCAAAGGCAGTGAGTGC+TGG + chr7.3:51034712-51034731 MS.gene051263:CDS 50.0%
GAAGGAAACAACGGGAGAAG+CGG - chr7.3:51033864-51033883 None:intergenic 50.0%
GGTGAAGTAGCTGAGTGTGA+AGG + chr7.3:51034748-51034767 MS.gene051263:CDS 50.0%
TCTGCTGAACTCAAGATCGC+TGG + chr7.3:51034028-51034047 MS.gene051263:CDS 50.0%
TGGAACAGTGTGAAGAGTGG+TGG + chr7.3:51034643-51034662 MS.gene051263:intron 50.0%
! CACAACAGCGTCGTTAGCTT+TGG - chr7.3:51033921-51033940 None:intergenic 50.0%
! GAACGCTTTTGCTGCTGAAG+AGG + chr7.3:51033949-51033968 MS.gene051263:CDS 50.0%
!!! GGCACCGTTTTGAGGAGTTT+CGG - chr7.3:51033831-51033850 None:intergenic 50.0%
CTGAGGCTTGAAAGCGACGA+AGG - chr7.3:51033882-51033901 None:intergenic 55.0%
GGAGCTTCAACGAAGAGCTG+AGG - chr7.3:51033899-51033918 None:intergenic 55.0%
GGGAGAAGCGGTTTCGGAAA+CGG - chr7.3:51033852-51033871 None:intergenic 55.0%
TGCGCTGAACCCTAAGCGTA+AGG + chr7.3:51033979-51033998 MS.gene051263:CDS 55.0%
! AGTGCTGGAGCAGTTGCTGA+AGG + chr7.3:51034727-51034746 MS.gene051263:CDS 55.0%
! TCGGAAACGGCACCGTTTTG+AGG - chr7.3:51033839-51033858 None:intergenic 55.0%
!! GAAAGCTGTTGGTGACGTGG+AGG + chr7.3:51034846-51034865 MS.gene051263:CDS 55.0%
! GAGTGTGAAGGCGCGTGTGG+TGG + chr7.3:51034760-51034779 MS.gene051263:CDS 65.0%
! GCTGAGTGTGAAGGCGCGTG+TGG + chr7.3:51034757-51034776 MS.gene051263:CDS 65.0%
! GTGAAGGCGCGTGTGGTGGT+GGG + chr7.3:51034764-51034783 MS.gene051263:CDS 65.0%
! TGTGAAGGCGCGTGTGGTGG+TGG + chr7.3:51034763-51034782 MS.gene051263:CDS 65.0%
!! TGGTGGTGGGCGCGTGGGAA+AGG + chr7.3:51034777-51034796 MS.gene051263:CDS 70.0%
!! GCGTGTGGTGGTGGGCGCGT+GGG + chr7.3:51034772-51034791 MS.gene051263:CDS 75.0%
!!! CGCGTGTGGTGGTGGGCGCG+TGG + chr7.3:51034771-51034790 MS.gene051263:CDS 80.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 51033821 51034873 51033821 ID=MS.gene051263
chr7.3 mRNA 51033821 51034873 51033821 ID=MS.gene051263.t1;Parent=MS.gene051263
chr7.3 exon 51033821 51034095 51033821 ID=MS.gene051263.t1.exon1;Parent=MS.gene051263.t1
chr7.3 CDS 51033821 51034095 51033821 ID=cds.MS.gene051263.t1;Parent=MS.gene051263.t1
chr7.3 exon 51034651 51034873 51034651 ID=MS.gene051263.t1.exon2;Parent=MS.gene051263.t1
chr7.3 CDS 51034651 51034873 51034651 ID=cds.MS.gene051263.t1;Parent=MS.gene051263.t1
Gene Sequence

>MS.gene051263

ATGGCTTCCGAAACTCCTCAAAACGGTGCCGTTTCCGAAACCGCTTCTCCCGTTGTTTCCTTCGTCGCTTTCAAGCCTCAGCTCTTCGTTGAAGCTCCCAAAGCTAACGACGCTGTTGTGTTCTACAAGAACGCTTTTGCTGCTGAAGAGGTTTCTCGTGCGCTGAACCCTAAGCGTAAGGCTGATCAAGAGCTTCCTCTTGTTCTCTCTGCTGAACTCAAGATCGCTGGTGCTTCCTTCCTTGTTGCTGATGCTGTTGATGATTCCGCTAAAACGTGAGTTTCTTTCTCGCTTTCTTCTTCCTTTTTTGTTCTAGATCTGAAAATGTTATAGCATTTTTTTATTTTCTTACATTTTTTCTCTTTTTTTTTTTACATTTTTTCTGTATTTTTCCATAGTCGTGATTTTCGTTTTGTTTTTATATGCTTCCGTAGTTATTTCATTACATTTGTGATATTTTATTTTTATATGAATGTTATGTCATGTTTATAGTTAGGAAATATTTTGTATTATTTTTTTATATTATTTTGGAATTTTCTGTTTATTATATGCTTTGATCTGTTTTTAGATAAGGTTATTTTTCTTTTTGTCCTTATATTATGCTACAGTTTTTTGTTTTGTGCTAGTTTCCGTAATCTTTGATTTTGTTTTTATTTCCTTACTTTTATTTTACTGTTTTATTTTGTATTTTATTTGATTTTTACATTTTTATTTGTTTTTTTAATGAAAGTTAGATTTTCTTTTTTGTCTCTTCGTAGAGCTTTTTATTTTCTACTCAGTAGTGTCCTTAATATTTATTCATTTTTTTTTTATAATTATTTTTGGAACAGTGTGAAGAGTGGTGGAAACGGTGTCGTTTTCTGTCTTGAATCTGAGAACATTGAAGCTGCGATAGCAAAGGCAGTGAGTGCTGGAGCAGTTGCTGAAGGTGAAGTAGCTGAGTGTGAAGGCGCGTGTGGTGGTGGGCGCGTGGGAAAGGTGACTGATCCATACGGTTACGTTTGGCAATTCTGCACTCCTGCAAAGAAAGCTGTTGGTGACGTGGAGGCTTGA

Protein sequence

>MS.gene051263.t1

MASETPQNGAVSETASPVVSFVAFKPQLFVEAPKANDAVVFYKNAFAAEEVSRALNPKRKADQELPLVLSAELKIAGASFLVADAVDDSAKTVKSGGNGVVFCLESENIEAAIAKAVSAGAVAEGEVAECEGACGGGRVGKVTDPYGYVWQFCTPAKKAVGDVEA