AlfalfaGEDB | MS.gene23313

M. sativa cultivar XinJiangDaYe / MS.gene23313

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23313.t1	XP_003606980.1	91.7	156	13	0	1	156	1	156	3.20E-50	208

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23313.t1	Q9LZP8	52.3	86	41	0	17	102	12	97	6.4e-15	82.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23313.t1	G7JIJ4	91.7	156	13	0	1	156	1	156	2.3e-50	208.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene23313.t1	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054468	MS.gene23313	0.807665	4.52E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23313.t1	MTR_4g070860	96.795	156	5	0	1	156	1	156	2.25e-107	303
MS.gene23313.t1	MTR_1g022495	59.006	161	60	4	1	156	1	160	1.34e-59	182
MS.gene23313.t1	MTR_3g117120	66.667	153	48	1	1	153	1	150	3.96e-57	176
MS.gene23313.t1	MTR_8g086020	54.938	162	65	5	1	156	1	160	5.41e-50	157
MS.gene23313.t1	MTR_3g112220	68.354	79	25	0	27	105	1	79	2.77e-33	113
MS.gene23313.t1	MTR_1g087040	37.121	132	68	2	17	148	14	130	2.65e-23	89.7
MS.gene23313.t1	MTR_7g029400	48.485	99	47	2	1	98	57	152	2.96e-21	85.5
MS.gene23313.t1	MTR_8g015250	53.012	83	38	1	17	98	60	142	5.11e-21	84.7
MS.gene23313.t1	MTR_6g016375	51.351	74	36	0	25	98	85	158	4.09e-18	77.4
MS.gene23313.t1	MTR_4g097440	37.838	111	67	1	1	109	44	154	6.91e-18	76.3
MS.gene23313.t1	MTR_7g017880	48.750	80	41	0	19	98	76	155	2.03e-17	75.5
MS.gene23313.t1	MTR_5g015090	45.679	81	44	0	27	107	58	138	1.86e-15	69.7
MS.gene23313.t1	MTR_3g109310	36.111	108	65	1	6	109	98	205	1.06e-13	67.0
MS.gene23313.t1	MTR_7g109510	51.807	83	40	0	27	109	19	101	2.48e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23313.t1	AT4G34590	60.714	140	47	4	24	156	21	159	1.49e-48	154
MS.gene23313.t1	AT1G75390	53.846	156	66	3	2	151	9	164	3.76e-47	151
MS.gene23313.t1	AT2G18160	49.655	145	57	4	27	156	28	171	9.41e-39	129
MS.gene23313.t1	AT1G75390	59.794	97	35	2	2	94	9	105	2.60e-32	112
MS.gene23313.t1	AT3G62420	44.615	130	67	1	25	154	20	144	3.76e-29	104
MS.gene23313.t1	AT5G15830	46.591	88	47	0	26	113	70	157	7.27e-19	79.3
MS.gene23313.t1	AT3G30530	53.425	73	34	0	26	98	77	149	1.11e-18	78.6
MS.gene23313.t1	AT1G13600	47.059	85	43	1	16	98	70	154	1.29e-18	79.0
MS.gene23313.t1	AT2G04038	48.810	84	41	1	17	98	59	142	2.12e-18	77.4
MS.gene23313.t1	AT5G38800	48.750	80	41	0	19	98	61	140	8.23e-17	73.2
MS.gene23313.t1	AT5G49450	35.455	110	57	1	1	110	1	96	9.20e-13	62.4
MS.gene23313.t1	AT4G37730	42.500	80	39	1	26	98	193	272	5.82e-12	62.4
MS.gene23313.t1	AT2G22850	41.250	80	40	1	26	98	125	204	3.90e-11	59.3
MS.gene23313.t1	AT2G22850	41.250	80	40	1	26	98	125	204	3.90e-11	59.3
MS.gene23313.t1	AT1G68880	43.284	67	38	0	28	94	45	111	7.45e-11	57.0

Find 27 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATATGAACCAGCCTATTA+TGG	0.236275	4.4:-37615540	MS.gene23313:CDS
AACTCCATTGCTTGAATTCA+AGG	0.317165	4.4:+37615631	None:intergenic
TCATCAAGTGGAACATCTTC+AGG	0.318453	4.4:-37615956	MS.gene23313:CDS
AGAAGAATTACAGGCTATAA+TGG	0.323452	4.4:-37615903	MS.gene23313:CDS
AGGTTCAGAAGAAGAATTAC+AGG	0.357684	4.4:-37615912	MS.gene23313:CDS
ATATCTGCAGAAGCCATAAT+AGG	0.368038	4.4:+37615527	None:intergenic
AGATGGGAGAGTTGAGCAAC+AGG	0.420852	4.4:-37615682	MS.gene23313:CDS
TGGATCAGAGAAAGAGGAAG+AGG	0.433685	4.4:-37615883	MS.gene23313:CDS
GCGAATCGGCAAGGCGGTCT+AGG	0.434095	4.4:-37615847	MS.gene23313:CDS
AGCTGAGAATTCAGTGTTGA+GGG	0.459385	4.4:-37615708	MS.gene23313:CDS
TTCAGTGTTGAGGGCTCAGA+TGG	0.472933	4.4:-37615699	MS.gene23313:CDS
GAATCTTTGAATGAAATTGT+TGG	0.493743	4.4:-37615656	MS.gene23313:CDS
TAGACCGCCTTGCCGATTCG+CGG	0.495356	4.4:+37615849	None:intergenic
GATGATATCGAACCGCGAAT+CGG	0.496807	4.4:-37615861	MS.gene23313:CDS
CGGCAAGGCGGTCTAGGATG+AGG	0.519007	4.4:-37615841	MS.gene23313:CDS
CTCATCATGGCTTCATCAAG+TGG	0.531339	4.4:-37615968	None:intergenic
CTATAATGGATCAGAGAAAG+AGG	0.546848	4.4:-37615889	MS.gene23313:CDS
CTGCAGAAGCCATAATAGGC+TGG	0.561236	4.4:+37615531	None:intergenic
GGGAGAGTTGAGCAACAGGT+TGG	0.564792	4.4:-37615678	MS.gene23313:CDS
GATGAGGAAACAGAAGCACT+TGG	0.601620	4.4:-37615825	MS.gene23313:CDS
AAGCTGAGAATTCAGTGTTG+AGG	0.603402	4.4:-37615709	MS.gene23313:CDS
TATCGAACCGCGAATCGGCA+AGG	0.622907	4.4:-37615856	MS.gene23313:CDS
TGTCACAAGTTGCTAAGCTG+AGG	0.639621	4.4:-37615793	MS.gene23313:CDS
TCATCTCTACTACAAAACTC+AGG	0.649209	4.4:-37615932	MS.gene23313:CDS
TCAGTGTTGAGGGCTCAGAT+GGG	0.657665	4.4:-37615698	MS.gene23313:CDS
GGTGCCTTGAATTCAAGCAA+TGG	0.668976	4.4:-37615635	MS.gene23313:CDS
CGAACCGCGAATCGGCAAGG+CGG	0.745432	4.4:-37615853	MS.gene23313:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAATCTTTGAATGAAATTGT+TGG	-	chr4.4:37615820-37615839	MS.gene23313:CDS	25.0%
	AGAAGAATTACAGGCTATAA+TGG	-	chr4.4:37615573-37615592	MS.gene23313:CDS	30.0%
	TTATATGAACCAGCCTATTA+TGG	-	chr4.4:37615936-37615955	MS.gene23313:CDS	30.0%
!	AACACTCAAATACTTTTGAG+TGG	+	chr4.4:37615746-37615765	None:intergenic	30.0%
!	AATTCAAGCAATGGAGTTTT+TGG	-	chr4.4:37615850-37615869	MS.gene23313:CDS	30.0%
!	GCTTTTGTTGAGTCAAATAA+TGG	-	chr4.4:37615883-37615902	MS.gene23313:CDS	30.0%
	AACTCCATTGCTTGAATTCA+AGG	+	chr4.4:37615848-37615867	None:intergenic	35.0%
	AGGTTCAGAAGAAGAATTAC+AGG	-	chr4.4:37615564-37615583	MS.gene23313:CDS	35.0%
	ATATCTGCAGAAGCCATAAT+AGG	+	chr4.4:37615952-37615971	None:intergenic	35.0%
	TCATCTCTACTACAAAACTC+AGG	-	chr4.4:37615544-37615563	MS.gene23313:CDS	35.0%
!!	CTATAATGGATCAGAGAAAG+AGG	-	chr4.4:37615587-37615606	MS.gene23313:CDS	35.0%
	AAGCTGAGAATTCAGTGTTG+AGG	-	chr4.4:37615767-37615786	MS.gene23313:CDS	40.0%
	TCATCAAGTGGAACATCTTC+AGG	-	chr4.4:37615520-37615539	MS.gene23313:CDS	40.0%
!	AGCTGAGAATTCAGTGTTGA+GGG	-	chr4.4:37615768-37615787	MS.gene23313:CDS	40.0%
	GATGATATCGAACCGCGAAT+CGG	-	chr4.4:37615615-37615634	MS.gene23313:CDS	45.0%
	GGTGCCTTGAATTCAAGCAA+TGG	-	chr4.4:37615841-37615860	MS.gene23313:CDS	45.0%
	TGGATCAGAGAAAGAGGAAG+AGG	-	chr4.4:37615593-37615612	MS.gene23313:CDS	45.0%
	TGTCACAAGTTGCTAAGCTG+AGG	-	chr4.4:37615683-37615702	MS.gene23313:CDS	45.0%
!!	GATGAGGAAACAGAAGCACT+TGG	-	chr4.4:37615651-37615670	MS.gene23313:CDS	45.0%
	AGATGGGAGAGTTGAGCAAC+AGG	-	chr4.4:37615794-37615813	MS.gene23313:CDS	50.0%
	CTGCAGAAGCCATAATAGGC+TGG	+	chr4.4:37615948-37615967	None:intergenic	50.0%
!	TCAGTGTTGAGGGCTCAGAT+GGG	-	chr4.4:37615778-37615797	MS.gene23313:CDS	50.0%
!	TTCAGTGTTGAGGGCTCAGA+TGG	-	chr4.4:37615777-37615796	MS.gene23313:CDS	50.0%
	GGGAGAGTTGAGCAACAGGT+TGG	-	chr4.4:37615798-37615817	MS.gene23313:CDS	55.0%
	TATCGAACCGCGAATCGGCA+AGG	-	chr4.4:37615620-37615639	MS.gene23313:CDS	55.0%
!!	TAGACCGCCTTGCCGATTCG+CGG	+	chr4.4:37615630-37615649	None:intergenic	60.0%
	CGAACCGCGAATCGGCAAGG+CGG	-	chr4.4:37615623-37615642	MS.gene23313:CDS	65.0%
	GCGAATCGGCAAGGCGGTCT+AGG	-	chr4.4:37615629-37615648	MS.gene23313:CDS	65.0%
!	CGGCAAGGCGGTCTAGGATG+AGG	-	chr4.4:37615635-37615654	MS.gene23313:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	37615514	37615984	37615514	ID=MS.gene23313
chr4.4	mRNA	37615514	37615984	37615514	ID=MS.gene23313.t1;Parent=MS.gene23313
chr4.4	exon	37615514	37615984	37615514	ID=MS.gene23313.t1.exon1;Parent=MS.gene23313.t1
chr4.4	CDS	37615514	37615984	37615514	ID=cds.MS.gene23313.t1;Parent=MS.gene23313.t1

Gene Sequence

>MS.gene23313

ATGGCTTCATCAAGTGGAACATCTTCAGGTTCATCTCTACTACAAAACTCAGGTTCAGAAGAAGAATTACAGGCTATAATGGATCAGAGAAAGAGGAAGAGGATGATATCGAACCGCGAATCGGCAAGGCGGTCTAGGATGAGGAAACAGAAGCACTTGGATGATCTTGTGTCACAAGTTGCTAAGCTGAGGAAAGAGAATCAAGAAATACTCACAAATGTTAACATCACCACTCAAAAGTATTTGAGTGTTGAAGCTGAGAATTCAGTGTTGAGGGCTCAGATGGGAGAGTTGAGCAACAGGTTGGAATCTTTGAATGAAATTGTTGGTGCCTTGAATTCAAGCAATGGAGTTTTTGGAGCATCAAATGCTTTTGTTGAGTCAAATAATGGTTTCTTCTTCAACTCTTTGAATAATATGTCTTATATGAACCAGCCTATTATGGCTTCTGCAGATATTTTGCAGTATTGA

Protein sequence

>MS.gene23313.t1

MASSSGTSSGSSLLQNSGSEEELQAIMDQRKRKRMISNRESARRSRMRKQKHLDDLVSQVAKLRKENQEILTNVNITTQKYLSVEAENSVLRAQMGELSNRLESLNEIVGALNSSNGVFGASNAFVESNNGFFFNSLNNMSYMNQPIMASADILQY