Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23888.t1 | CBH32561.1 | 95.7 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 3.10E-35 | 157.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23888.t1 | P68429 | 95.7 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 7.4e-38 | 157.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23888.t1 | B2ZF73 | 89.7 | 97 | 10 | 0 | 1 | 97 | 1 | 97 | 2.2e-35 | 157.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055957 | MS.gene23888 | 0.824792 | 6.66E-54 | -1.69E-46 |
| MS.gene056041 | MS.gene23888 | 0.803267 | 3.78E-49 | -1.69E-46 |
| MS.gene06064 | MS.gene23888 | 0.810785 | 9.70E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23888.t1 | MTR_7g059070 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_5g029820 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_5g029770 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_4g065990 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_4g088150 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_2g035230 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_8g092720 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_8g063500 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_8g103245 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_8g092820 | 95.652 | 92 | 4 | 0 | 1 | 92 | 1 | 92 | 8.92e-57 | 171 |
| MS.gene23888.t1 | MTR_7g013610 | 95.652 | 92 | 4 | 0 | 1 | 92 | 100 | 191 | 1.11e-56 | 174 |
| MS.gene23888.t1 | MTR_4g097175 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.76e-54 | 166 |
| MS.gene23888.t1 | MTR_1g023630 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.76e-54 | 166 |
| MS.gene23888.t1 | MTR_8g061940 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.76e-54 | 166 |
| MS.gene23888.t1 | MTR_8g099430 | 91.304 | 92 | 8 | 0 | 1 | 92 | 1 | 92 | 7.15e-54 | 164 |
| MS.gene23888.t1 | MTR_4g097170 | 91.304 | 92 | 8 | 0 | 1 | 92 | 42 | 133 | 5.42e-53 | 163 |
| MS.gene23888.t1 | MTR_2g082310 | 85.870 | 92 | 13 | 0 | 1 | 92 | 1 | 92 | 1.32e-50 | 156 |
| MS.gene23888.t1 | MTR_2g082340 | 85.870 | 92 | 13 | 0 | 1 | 92 | 1 | 92 | 1.32e-50 | 156 |
| MS.gene23888.t1 | MTR_1g023630 | 90.361 | 83 | 8 | 0 | 1 | 83 | 1 | 83 | 1.90e-48 | 149 |
| MS.gene23888.t1 | MTR_2g082370 | 75.490 | 102 | 15 | 1 | 1 | 92 | 1 | 102 | 1.32e-43 | 139 |
| MS.gene23888.t1 | MTR_2g082400 | 75.269 | 93 | 21 | 2 | 1 | 92 | 1 | 92 | 1.03e-39 | 128 |
| MS.gene23888.t1 | MTR_0197s0100 | 93.939 | 66 | 4 | 0 | 1 | 66 | 1 | 66 | 3.23e-36 | 120 |
| MS.gene23888.t1 | MTR_7g013600 | 83.824 | 68 | 11 | 0 | 25 | 92 | 2 | 69 | 4.38e-34 | 113 |
| MS.gene23888.t1 | MTR_5g024630 | 63.043 | 92 | 31 | 2 | 1 | 92 | 1 | 89 | 7.82e-27 | 95.5 |
| MS.gene23888.t1 | MTR_7g023830 | 59.783 | 92 | 36 | 1 | 1 | 92 | 1 | 91 | 2.34e-26 | 94.7 |
| MS.gene23888.t1 | MTR_7g023880 | 51.485 | 101 | 34 | 6 | 1 | 92 | 1 | 95 | 6.11e-18 | 73.6 |
| MS.gene23888.t1 | MTR_5g024530 | 52.564 | 78 | 26 | 3 | 19 | 92 | 68 | 138 | 1.32e-13 | 62.8 |
| MS.gene23888.t1 | MTR_5g024600 | 57.407 | 54 | 23 | 0 | 1 | 54 | 1 | 54 | 6.56e-13 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23888.t1 | AT5G65360 | 94.565 | 92 | 5 | 0 | 1 | 92 | 1 | 92 | 2.99e-56 | 170 |
| MS.gene23888.t1 | AT5G10390 | 94.565 | 92 | 5 | 0 | 1 | 92 | 1 | 92 | 2.99e-56 | 170 |
| MS.gene23888.t1 | AT5G10400 | 94.565 | 92 | 5 | 0 | 1 | 92 | 1 | 92 | 2.99e-56 | 170 |
| MS.gene23888.t1 | AT3G27360 | 94.565 | 92 | 5 | 0 | 1 | 92 | 1 | 92 | 2.99e-56 | 170 |
| MS.gene23888.t1 | AT1G09200 | 94.565 | 92 | 5 | 0 | 1 | 92 | 1 | 92 | 2.99e-56 | 170 |
| MS.gene23888.t1 | AT5G10980 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.84e-54 | 166 |
| MS.gene23888.t1 | AT4G40040 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.84e-54 | 166 |
| MS.gene23888.t1 | AT4G40040 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.84e-54 | 166 |
| MS.gene23888.t1 | AT4G40040 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.84e-54 | 166 |
| MS.gene23888.t1 | AT4G40030 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.84e-54 | 166 |
| MS.gene23888.t1 | AT4G40030 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.84e-54 | 166 |
| MS.gene23888.t1 | AT4G40030 | 92.391 | 92 | 7 | 0 | 1 | 92 | 1 | 92 | 1.84e-54 | 166 |
| MS.gene23888.t1 | AT4G40030 | 92.391 | 92 | 7 | 0 | 1 | 92 | 29 | 120 | 2.99e-54 | 166 |
| MS.gene23888.t1 | AT5G65350 | 89.130 | 92 | 10 | 0 | 1 | 92 | 1 | 92 | 1.14e-52 | 161 |
| MS.gene23888.t1 | AT1G75600 | 88.043 | 92 | 11 | 0 | 1 | 92 | 1 | 92 | 2.14e-51 | 158 |
| MS.gene23888.t1 | AT1G13370 | 88.043 | 92 | 11 | 0 | 1 | 92 | 1 | 92 | 3.36e-51 | 157 |
| MS.gene23888.t1 | AT1G19890 | 83.871 | 93 | 14 | 1 | 1 | 92 | 1 | 93 | 1.37e-48 | 151 |
| MS.gene23888.t1 | AT5G12910 | 64.130 | 92 | 28 | 2 | 1 | 92 | 1 | 87 | 1.28e-33 | 113 |
Find 30 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTTGCCGGAGCAGATTTC+CGG | 0.243304 | 5.3:+19107145 | None:intergenic |
| TTGGTGGCTAGCTGCTTTCT+TGG | 0.277587 | 5.3:+19107174 | None:intergenic |
| TTCTTGACTCCTCCGGTTGC+CGG | 0.286082 | 5.3:+19107132 | None:intergenic |
| TCACGAAGTGCAACGGTTCC+TGG | 0.291776 | 5.3:+19107093 | None:intergenic |
| TGAGAAGCTCAGTGCTCTTC+TGG | 0.304985 | 5.3:+19107058 | None:intergenic |
| AGCAGATTTCCGGGCAGCTT+TGG | 0.390197 | 5.3:+19107155 | None:intergenic |
| CGGAAATCTGCTCCGGCAAC+CGG | 0.392471 | 5.3:-19107144 | MS.gene23888:CDS |
| GGTTGCCGGAGCAGATTTCC+GGG | 0.407337 | 5.3:+19107146 | None:intergenic |
| CTTTCTTGGTGCCTTTCCTC+CGG | 0.425692 | 5.3:+19107188 | None:intergenic |
| CGATTTCACGAACAAGTCTC+TGG | 0.432729 | 5.3:+19107019 | None:intergenic |
| AAACAGCGCTGCTTTGAAAT+CGG | 0.457546 | 5.3:+19106974 | None:intergenic |
| AGATTTCCGGGCAGCTTTGG+TGG | 0.468887 | 5.3:+19107158 | None:intergenic |
| TGTGGCTTTCTTGACTCCTC+CGG | 0.481121 | 5.3:+19107125 | None:intergenic |
| AGCTAGCCACCAAAGCTGCC+CGG | 0.491500 | 5.3:-19107164 | MS.gene23888:CDS |
| AGCTGCCCGGAAATCTGCTC+CGG | 0.505877 | 5.3:-19107151 | MS.gene23888:CDS |
| GCTTCAGCAGCTTCTTGAAG+AGG | 0.523444 | 5.3:+19106948 | None:intergenic |
| AGAGCACTGAGCTTCTCATA+AGG | 0.531497 | 5.3:-19107053 | MS.gene23888:CDS |
| TCGCAAATCCACCGGAGGAA+AGG | 0.532804 | 5.3:-19107199 | MS.gene23888:CDS |
| TGTTTGCTTTGTACGAGCCA+TGG | 0.537743 | 5.3:+19107224 | None:intergenic |
| ACTTGTTCGTGAAATCGCTC+AGG | 0.551065 | 5.3:-19107013 | MS.gene23888:CDS |
| TCTTGGTGCCTTTCCTCCGG+TGG | 0.556160 | 5.3:+19107191 | None:intergenic |
| CAGTGCTCTTCTGGTACTTG+CGG | 0.579006 | 5.3:+19107067 | None:intergenic |
| CAAACAGCTCGCAAATCCAC+CGG | 0.586123 | 5.3:-19107207 | MS.gene23888:CDS |
| TCACGAACAAGTCTCTGGAA+TGG | 0.590006 | 5.3:+19107024 | None:intergenic |
| AAAGCCACACAGATCCGTCC+AGG | 0.596363 | 5.3:-19107111 | MS.gene23888:CDS |
| AAATCTGCTCCGGCAACCGG+AGG | 0.607496 | 5.3:-19107141 | MS.gene23888:CDS |
| GCGGATCTCACGAAGTGCAA+CGG | 0.633057 | 5.3:+19107086 | None:intergenic |
| GAAGTGCAACGGTTCCTGGA+CGG | 0.655834 | 5.3:+19107097 | None:intergenic |
| GGTTCCTGGACGGATCTGTG+TGG | 0.691842 | 5.3:+19107107 | None:intergenic |
| ACAGCTCGCAAATCCACCGG+AGG | 0.704783 | 5.3:-19107204 | MS.gene23888:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAGCGCTGCTTTGAAAT+CGG | + | chr5.3:19107202-19107221 | None:intergenic | 40.0% |
| ACTTGTTCGTGAAATCGCTC+AGG | - | chr5.3:19107160-19107179 | MS.gene23888:CDS | 45.0% | |
| CGATTTCACGAACAAGTCTC+TGG | + | chr5.3:19107157-19107176 | None:intergenic | 45.0% | |
| ! | TCACGAACAAGTCTCTGGAA+TGG | + | chr5.3:19107152-19107171 | None:intergenic | 45.0% |
| !! | AGAGCACTGAGCTTCTCATA+AGG | - | chr5.3:19107120-19107139 | MS.gene23888:CDS | 45.0% |
| CAAACAGCTCGCAAATCCAC+CGG | - | chr5.3:19106966-19106985 | MS.gene23888:CDS | 50.0% | |
| CAGTGCTCTTCTGGTACTTG+CGG | + | chr5.3:19107109-19107128 | None:intergenic | 50.0% | |
| TGAGAAGCTCAGTGCTCTTC+TGG | + | chr5.3:19107118-19107137 | None:intergenic | 50.0% | |
| TGTGGCTTTCTTGACTCCTC+CGG | + | chr5.3:19107051-19107070 | None:intergenic | 50.0% | |
| ! | TTGGTGGCTAGCTGCTTTCT+TGG | + | chr5.3:19107002-19107021 | None:intergenic | 50.0% |
| !! | CTTTCTTGGTGCCTTTCCTC+CGG | + | chr5.3:19106988-19107007 | None:intergenic | 50.0% |
| AAAGCCACACAGATCCGTCC+AGG | - | chr5.3:19107062-19107081 | MS.gene23888:CDS | 55.0% | |
| AGCAGATTTCCGGGCAGCTT+TGG | + | chr5.3:19107021-19107040 | None:intergenic | 55.0% | |
| GAAGTGCAACGGTTCCTGGA+CGG | + | chr5.3:19107079-19107098 | None:intergenic | 55.0% | |
| GCGGATCTCACGAAGTGCAA+CGG | + | chr5.3:19107090-19107109 | None:intergenic | 55.0% | |
| TCACGAAGTGCAACGGTTCC+TGG | + | chr5.3:19107083-19107102 | None:intergenic | 55.0% | |
| TCGCAAATCCACCGGAGGAA+AGG | - | chr5.3:19106974-19106993 | MS.gene23888:CDS | 55.0% | |
| TTCTTGACTCCTCCGGTTGC+CGG | + | chr5.3:19107044-19107063 | None:intergenic | 55.0% | |
| ! | AGATTTCCGGGCAGCTTTGG+TGG | + | chr5.3:19107018-19107037 | None:intergenic | 55.0% |
| AAATCTGCTCCGGCAACCGG+AGG | - | chr5.3:19107032-19107051 | MS.gene23888:CDS | 60.0% | |
| ACAGCTCGCAAATCCACCGG+AGG | - | chr5.3:19106969-19106988 | MS.gene23888:CDS | 60.0% | |
| AGCTAGCCACCAAAGCTGCC+CGG | - | chr5.3:19107009-19107028 | MS.gene23888:CDS | 60.0% | |
| AGCTGCCCGGAAATCTGCTC+CGG | - | chr5.3:19107022-19107041 | MS.gene23888:CDS | 60.0% | |
| CGGAAATCTGCTCCGGCAAC+CGG | - | chr5.3:19107029-19107048 | MS.gene23888:CDS | 60.0% | |
| CGGTTGCCGGAGCAGATTTC+CGG | + | chr5.3:19107031-19107050 | None:intergenic | 60.0% | |
| GGTTCCTGGACGGATCTGTG+TGG | + | chr5.3:19107069-19107088 | None:intergenic | 60.0% | |
| GGTTGCCGGAGCAGATTTCC+GGG | + | chr5.3:19107030-19107049 | None:intergenic | 60.0% | |
| !! | TCTTGGTGCCTTTCCTCCGG+TGG | + | chr5.3:19106985-19107004 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 19106951 | 19107244 | 19106951 | ID=MS.gene23888 |
| chr5.3 | mRNA | 19106951 | 19107244 | 19106951 | ID=MS.gene23888.t1;Parent=MS.gene23888 |
| chr5.3 | exon | 19106951 | 19107244 | 19106951 | ID=MS.gene23888.t1.exon1;Parent=MS.gene23888.t1 |
| chr5.3 | CDS | 19106951 | 19107244 | 19106951 | ID=cds.MS.gene23888.t1;Parent=MS.gene23888.t1 |
| Gene Sequence |
| Protein sequence |
