AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23959

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23959.t1 MTR_1g069765 99.283 279 2 0 1 279 1 279 0.0 567
MS.gene23959.t1 MTR_2g091185 43.017 179 92 3 5 180 18 189 2.25e-41 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23959.t1 AT1G03900 70.545 275 62 6 10 279 12 272 9.69e-119 341
MS.gene23959.t1 AT3G58600 43.575 179 85 4 5 177 27 195 7.45e-40 140

Find 87 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGGAAGCATGCGTTTAT+TGG 0.243287 1.3:-49332997 MS.gene23959:CDS
GCTGGCATGCTTTCAGCTGC+TGG 0.245848 1.3:-49332179 MS.gene23959:CDS
ATCGGATCTTTCTCAATTAC+AGG 0.250114 1.3:-49331964 MS.gene23959:intron
GGTCCTGTATCTAGTGTTAA+AGG 0.284346 1.3:-49332023 MS.gene23959:CDS
GGAGCAAGGTTCAATACTTT+TGG 0.310945 1.3:+49332125 None:intergenic
AAGCATGCGTTTATTGGATT+GGG 0.336786 1.3:-49332991 MS.gene23959:CDS
CACAAGGCGGCTGGTGGAGC+TGG 0.355323 1.3:-49332197 MS.gene23959:CDS
GCCGTGACCGGTGCGAGATC+CGG 0.369388 1.3:-49333143 MS.gene23959:CDS
GCGAAGAGATCACCGGAGTT+AGG 0.375730 1.3:+49333111 None:intergenic
GCTTTCAGCTGCTGGCCTAT+CGG 0.386640 1.3:-49332171 MS.gene23959:CDS
CTTTCAGCTGCTGGCCTATC+GGG 0.396419 1.3:-49332170 MS.gene23959:CDS
TCCCGGTCAGCGTGAGGGTT+CGG 0.398042 1.3:-49333073 MS.gene23959:CDS
CGAGCAGCAACCGGATCATT+TGG 0.402087 1.3:+49332059 None:intergenic
TTCATCCTGCTGTTAATCAT+AGG 0.414816 1.3:-49332843 MS.gene23959:CDS
TGCTGTTAATCATAGGCTTA+AGG 0.419472 1.3:-49332836 MS.gene23959:intron
AACCGAACCCTCACGCTGAC+CGG 0.428541 1.3:+49333071 None:intergenic
CTTCCCTCATCAGCCACTCC+TGG 0.429001 1.3:-49331525 MS.gene23959:CDS
GTCCCTTTAACACTAGATAC+AGG 0.431990 1.3:+49332020 None:intergenic
GAAGCATGCGTTTATTGGAT+TGG 0.436801 1.3:-49332992 MS.gene23959:CDS
GCGTGAGGGTTCGGTTGAAC+CGG 0.442496 1.3:-49333064 MS.gene23959:CDS
TGTGAGAAGAGAACATGATA+AGG 0.442504 1.3:-49332908 MS.gene23959:CDS
GGCTCCAATCAGACAAGATC+TGG 0.444379 1.3:-49333191 MS.gene23959:CDS
ATCCCGCCGCGAACAACCTC+CGG 0.448428 1.3:-49333234 MS.gene23959:CDS
TTTGTTCTGAAGATTGAAGA+TGG 0.449673 1.3:-49333021 MS.gene23959:CDS
CCTGGATCAACACCTGCATC+AGG 0.454323 1.3:-49331507 MS.gene23959:CDS
CGCCGGAGGTTGTTCGCGGC+GGG 0.458516 1.3:+49333232 None:intergenic
AGAGAACATGATAAGGAGTC+TGG 0.464167 1.3:-49332901 MS.gene23959:CDS
CCTGATGCAGGTGTTGATCC+AGG 0.466664 1.3:+49331507 None:intergenic
GAAGCAATCCGAGCAGCAAC+CGG 0.468103 1.3:+49332050 None:intergenic
ACCGGATCATTTGGTGGGAG+TGG 0.474405 1.3:+49332068 None:intergenic
GTGCTTGACATTAATCCTGA+TGG 0.479670 1.3:+49332217 None:intergenic
TCCACTCCCACCAAATGATC+CGG 0.488066 1.3:-49332069 MS.gene23959:CDS
CGGAGTTAGGATCTTCTAAC+CGG 0.488892 1.3:+49333124 None:intergenic
TAACCGCCGGAGGTTGTTCG+CGG 0.489732 1.3:+49333228 None:intergenic
GCGTCGTATCTCGCCGTGAC+CGG 0.490086 1.3:-49333155 MS.gene23959:CDS
TATCTCGACGAATCGAGAAC+CGG 0.491919 1.3:+49333045 None:intergenic
GTCCTGTATCTAGTGTTAAA+GGG 0.497916 1.3:-49332022 MS.gene23959:CDS
CGGACCAGATCTTGTCTGAT+TGG 0.500982 1.3:+49333187 None:intergenic
GTTTATTGGATTGGGGTTTG+CGG 0.504982 1.3:-49332983 MS.gene23959:CDS
CAATCAGACAAGATCTGGTC+CGG 0.507755 1.3:-49333186 MS.gene23959:CDS
AGTGTTAAAGGGACAAAAGA+AGG 0.515401 1.3:-49332011 MS.gene23959:CDS
ACCGGATCTCGCACCGGTCA+CGG 0.515952 1.3:+49333142 None:intergenic
AGATACGACGCGGAGACGGC+CGG 0.516487 1.3:+49333167 None:intergenic
AAGGCTGCCCATCCTGATGC+AGG 0.520170 1.3:+49331495 None:intergenic
GTCGTTAGAAGAAGAAGAAG+AGG 0.522046 1.3:-49333304 MS.gene23959:CDS
AATGTCAAGCACAAGGCGGC+TGG 0.531510 1.3:-49332206 MS.gene23959:CDS
ACCGAACCCTCACGCTGACC+GGG 0.532951 1.3:+49333072 None:intergenic
TGTAGGAGGGAGAAACCATC+AGG 0.535611 1.3:-49332232 MS.gene23959:intron
GTCAAGCACAAGGCGGCTGG+TGG 0.546548 1.3:-49332203 MS.gene23959:CDS
CAAATGATCCGGTTGCTGCT+CGG 0.548131 1.3:-49332058 MS.gene23959:CDS
CTGAAGATTGAAGATGGACA+AGG 0.551166 1.3:-49333015 MS.gene23959:CDS
GCTGCTCGGATTGCTTCTAC+CGG 0.551616 1.3:-49332044 MS.gene23959:CDS
AGCATGCGTTTATTGGATTG+GGG 0.552301 1.3:-49332990 MS.gene23959:CDS
TTCGCCGCATTTGTAACCGC+CGG 0.553188 1.3:+49333215 None:intergenic
ACAGTCTACTGATTCTCTAT+CGG 0.561613 1.3:-49331982 MS.gene23959:CDS
GATCAACACCTGCATCAGGA+TGG 0.567922 1.3:-49331503 MS.gene23959:CDS
TTGGATTGGGGTTTGCGGAG+AGG 0.569343 1.3:-49332978 MS.gene23959:CDS
ATCAACACCTGCATCAGGAT+GGG 0.573253 1.3:-49331502 MS.gene23959:CDS
CTTCTAACCGGATCTCGCAC+CGG 0.574822 1.3:+49333136 None:intergenic
CCGCCGGAGGTTGTTCGCGG+CGG 0.582317 1.3:+49333231 None:intergenic
GGCGAGATACGACGCGGAGA+CGG 0.582763 1.3:+49333163 None:intergenic
GCAGCAACCGGATCATTTGG+TGG 0.583616 1.3:+49332062 None:intergenic
GCGGTTACAAATGCGGCGAA+TGG 0.584085 1.3:-49333212 MS.gene23959:CDS
TTGCTACATGCACACCCGAT+AGG 0.586697 1.3:+49332156 None:intergenic
TCGCGGCGGGATCTTGTAGA+CGG 0.592219 1.3:+49333245 None:intergenic
GCCGCATTTGTAACCGCCGG+AGG 0.596884 1.3:+49333218 None:intergenic
TTAAGCCTATGATTAACAGC+AGG 0.600310 1.3:+49332838 None:intergenic
TGGCTGTATATATAATCAGA+AGG 0.603345 1.3:+49331476 None:intergenic
ACCTCCGGCGGTTACAAATG+CGG 0.604789 1.3:-49333219 MS.gene23959:CDS
TGATCCAGGAGTGGCTGATG+AGG 0.607039 1.3:+49331521 None:intergenic
GTCTGGTGATGCTGATGCCG+CGG 0.607238 1.3:-49332884 MS.gene23959:CDS
CGCGAACGACGAGGAGTGTG+TGG 0.610612 1.3:+49333274 None:intergenic
CAGCAACCGGATCATTTGGT+GGG 0.614621 1.3:+49332063 None:intergenic
CAGGATTAATGTCAAGCACA+AGG 0.616170 1.3:-49332213 MS.gene23959:CDS
CGGTTAGAAGATCCTAACTC+CGG 0.634275 1.3:-49333123 MS.gene23959:CDS
ACACGCAGCGAAGAGATCAC+CGG 0.637569 1.3:+49333104 None:intergenic
TTTAACACTAGATACAGGAC+CGG 0.645020 1.3:+49332025 None:intergenic
CGGCGACTTCGCGAACGACG+AGG 0.648082 1.3:+49333265 None:intergenic
ATTTGGTGGGAGTGGAAGAG+CGG 0.652140 1.3:+49332076 None:intergenic
TGCAGGTGTTGATCCAGGAG+TGG 0.652617 1.3:+49331512 None:intergenic
TGAAGATTGAAGATGGACAA+GGG 0.659170 1.3:-49333014 MS.gene23959:CDS
GATCCAGGAGTGGCTGATGA+GGG 0.665742 1.3:+49331522 None:intergenic
TGGTGATGCTGATGCCGCGG+AGG 0.679687 1.3:-49332881 MS.gene23959:CDS
CCGCCGCGAACAACCTCCGG+CGG 0.687466 1.3:-49333231 MS.gene23959:CDS
ATCGATCTGTGAATCCTCCG+CGG 0.729159 1.3:+49332867 None:intergenic
GATTAATGTCAAGCACAAGG+CGG 0.745005 1.3:-49332210 MS.gene23959:CDS
GGTCACGGCGAGATACGACG+CGG 0.826251 1.3:+49333157 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGATATAATTATGATCA+TGG - chr1.3:49332218-49332237 MS.gene23959:CDS 15.0%
!!! ATTAGTTTTTTGTGTATTTT+TGG - chr1.3:49332133-49332152 MS.gene23959:CDS 15.0%
!! ACAATATAGAGAAATTAAAC+TGG + chr1.3:49333186-49333205 None:intergenic 20.0%
!! TATATGCATAAACAAAGAAA+TGG + chr1.3:49333095-49333114 None:intergenic 20.0%
!!! GTAAACATGGTTTTAAAAAA+GGG + chr1.3:49332458-49332477 None:intergenic 20.0%
!!! TGAACTTTAATTTATTGTAG+CGG - chr1.3:49332037-49332056 MS.gene23959:CDS 20.0%
!!! TGTAAACATGGTTTTAAAAA+AGG + chr1.3:49332459-49332478 None:intergenic 20.0%
! AAAACAAATGAAGTGGAAAT+AGG + chr1.3:49332892-49332911 None:intergenic 25.0%
! AAAACCATGTTTACAAATCA+CGG - chr1.3:49332464-49332483 MS.gene23959:intron 25.0%
! ATTCATATTAAACAGTGTCT+GGG + chr1.3:49333215-49333234 None:intergenic 25.0%
! TTGTAAAACAACAGTTACAT+TGG - chr1.3:49333131-49333150 MS.gene23959:CDS 25.0%
!! ACAACCATGATTTAAAACAT+TGG + chr1.3:49332314-49332333 None:intergenic 25.0%
!!! AATGTTTTAAATCATGGTTG+TGG - chr1.3:49332313-49332332 MS.gene23959:intron 25.0%
!!! TTCTCCAATGTTTTAAATCA+TGG - chr1.3:49332307-49332326 MS.gene23959:intron 25.0%
AAATGAAGTGGAAATAGGAA+AGG + chr1.3:49332887-49332906 None:intergenic 30.0%
AGAACTTGCAAATTATTGCA+TGG - chr1.3:49332928-49332947 MS.gene23959:CDS 30.0%
AGCATAGCATAATCTTAGAT+AGG + chr1.3:49333028-49333047 None:intergenic 30.0%
AGTCACTATTGAGATTACAT+TGG + chr1.3:49332385-49332404 None:intergenic 30.0%
ATTTGGGCAAAAAATAACCT+CGG + chr1.3:49332252-49332271 None:intergenic 30.0%
CATAATCTTAGATAGGTACA+CGG + chr1.3:49333021-49333040 None:intergenic 30.0%
CTAGAAACTACTCTAAACAT+CGG + chr1.3:49332275-49332294 None:intergenic 30.0%
GATTCATATTAAACAGTGTC+TGG + chr1.3:49333216-49333235 None:intergenic 30.0%
GATTGCAAAAACAAATGAAG+TGG + chr1.3:49332899-49332918 None:intergenic 30.0%
TCGACAAATACTTCAAACAT+AGG + chr1.3:49332539-49332558 None:intergenic 30.0%
TTGGTTAGTGTTAATTGTTG+TGG - chr1.3:49332152-49332171 MS.gene23959:CDS 30.0%
! TGTTTGAAGTATTTGTCGAT+TGG - chr1.3:49332540-49332559 MS.gene23959:intron 30.0%
!! TGATCACACAAATTTGCTAA+TGG + chr1.3:49331993-49332012 None:intergenic 30.0%
!! TTTGTTCTGAAGATTGAAGA+TGG - chr1.3:49331776-49331795 MS.gene23959:intron 30.0%
AAGCATGCGTTTATTGGATT+GGG - chr1.3:49331806-49331825 MS.gene23959:intron 35.0%
ACTACTCTAAACATCGGATT+TGG + chr1.3:49332269-49332288 None:intergenic 35.0%
AGTGTTAAAGGGACAAAAGA+AGG - chr1.3:49332786-49332805 MS.gene23959:intron 35.0%
ATCGGATCTTTCTCAATTAC+AGG - chr1.3:49332833-49332852 MS.gene23959:intron 35.0%
CTACTCTAAACATCGGATTT+GGG + chr1.3:49332268-49332287 None:intergenic 35.0%
CTTCAAACATAGGATAAGCA+AGG + chr1.3:49332529-49332548 None:intergenic 35.0%
GTCCTGTATCTAGTGTTAAA+GGG - chr1.3:49332775-49332794 MS.gene23959:intron 35.0%
TAAAAAAGGGTTTGCGATCT+GGG + chr1.3:49332445-49332464 None:intergenic 35.0%
TCGTTAGAAGAAGAAGAAGA+GGG - chr1.3:49331494-49331513 MS.gene23959:CDS 35.0%
TGAAGATTGAAGATGGACAA+GGG - chr1.3:49331783-49331802 MS.gene23959:intron 35.0%
TGCTGTTAATCATAGGCTTA+AGG - chr1.3:49331961-49331980 MS.gene23959:intron 35.0%
TGTGAGAAGAGAACATGATA+AGG - chr1.3:49331889-49331908 MS.gene23959:intron 35.0%
TTAAAAAAGGGTTTGCGATC+TGG + chr1.3:49332446-49332465 None:intergenic 35.0%
TTAAGCCTATGATTAACAGC+AGG + chr1.3:49331962-49331981 None:intergenic 35.0%
TTCATCCTGCTGTTAATCAT+AGG - chr1.3:49331954-49331973 MS.gene23959:intron 35.0%
TTGCAAATTATTGCATGGCT+GGG - chr1.3:49332933-49332952 MS.gene23959:CDS 35.0%
TTTAACACTAGATACAGGAC+CGG + chr1.3:49332775-49332794 None:intergenic 35.0%
TTTCTCAATTACAGGTATGC+TGG - chr1.3:49332841-49332860 MS.gene23959:CDS 35.0%
! AAGACCGTGATTTGTAAACA+TGG + chr1.3:49332471-49332490 None:intergenic 35.0%
! TTGCTATTGCAAATGATCGT+GGG - chr1.3:49332350-49332369 MS.gene23959:intron 35.0%
!! ACAGTCTACTGATTCTCTAT+CGG - chr1.3:49332815-49332834 MS.gene23959:intron 35.0%
!!! AGGTTCAATACTTTTGGCTT+CGG + chr1.3:49332669-49332688 None:intergenic 35.0%
AGAGAACATGATAAGGAGTC+TGG - chr1.3:49331896-49331915 MS.gene23959:intron 40.0%
AGCATGCGTTTATTGGATTG+GGG - chr1.3:49331807-49331826 MS.gene23959:intron 40.0%
CAGGATTAATGTCAAGCACA+AGG - chr1.3:49332584-49332603 MS.gene23959:intron 40.0%
CTGAAGATTGAAGATGGACA+AGG - chr1.3:49331782-49331801 MS.gene23959:intron 40.0%
CTGGCAAAGTAACATGATCA+CGG + chr1.3:49333167-49333186 None:intergenic 40.0%
CTTGCAAATTATTGCATGGC+TGG - chr1.3:49332932-49332951 MS.gene23959:CDS 40.0%
GAAGCATGCGTTTATTGGAT+TGG - chr1.3:49331805-49331824 MS.gene23959:intron 40.0%
GATTAATGTCAAGCACAAGG+CGG - chr1.3:49332587-49332606 MS.gene23959:intron 40.0%
GGAAATAGGAAAGGCTCTTA+AGG + chr1.3:49332878-49332897 None:intergenic 40.0%
GGTCCTGTATCTAGTGTTAA+AGG - chr1.3:49332774-49332793 MS.gene23959:intron 40.0%
GTCCCTTTAACACTAGATAC+AGG + chr1.3:49332780-49332799 None:intergenic 40.0%
GTCGTTAGAAGAAGAAGAAG+AGG - chr1.3:49331493-49331512 MS.gene23959:CDS 40.0%
GTGCTTGACATTAATCCTGA+TGG + chr1.3:49332583-49332602 None:intergenic 40.0%
! GGAGCAAGGTTCAATACTTT+TGG + chr1.3:49332675-49332694 None:intergenic 40.0%
! GTTGCTATTGCAAATGATCG+TGG - chr1.3:49332349-49332368 MS.gene23959:intron 40.0%
! GTTTATTGGATTGGGGTTTG+CGG - chr1.3:49331814-49331833 MS.gene23959:intron 40.0%
AAGGCTCTTAAGGAATGAGC+AGG + chr1.3:49332868-49332887 None:intergenic 45.0%
ATCAACACCTGCATCAGGAT+GGG - chr1.3:49333295-49333314 MS.gene23959:CDS 45.0%
CAAATGATCGTGGGCAAATG+GGG - chr1.3:49332359-49332378 MS.gene23959:intron 45.0%
CAATCAGACAAGATCTGGTC+CGG - chr1.3:49331611-49331630 MS.gene23959:intron 45.0%
CGGAGTTAGGATCTTCTAAC+CGG + chr1.3:49331676-49331695 None:intergenic 45.0%
CGGTTAGAAGATCCTAACTC+CGG - chr1.3:49331674-49331693 MS.gene23959:intron 45.0%
GCAAATGATCGTGGGCAAAT+GGG - chr1.3:49332358-49332377 MS.gene23959:intron 45.0%
TATCTCGACGAATCGAGAAC+CGG + chr1.3:49331755-49331774 None:intergenic 45.0%
TGATCATGGTGAATTGTCCG+AGG - chr1.3:49332232-49332251 MS.gene23959:intron 45.0%
TGCAAATGATCGTGGGCAAA+TGG - chr1.3:49332357-49332376 MS.gene23959:intron 45.0%
! CAAGGGAAGCATGCGTTTAT+TGG - chr1.3:49331800-49331819 MS.gene23959:intron 45.0%
!! GTATTTGTCGATTGGCCTGT+AGG - chr1.3:49332548-49332567 MS.gene23959:intron 45.0%
!!! GCTGCGTGTTTTGTTTTTCC+CGG - chr1.3:49331707-49331726 MS.gene23959:intron 45.0%
ATCGATCTGTGAATCCTCCG+CGG + chr1.3:49331933-49331952 None:intergenic 50.0%
CAAATGATCCGGTTGCTGCT+CGG - chr1.3:49332739-49332758 MS.gene23959:intron 50.0%
CAGCAACCGGATCATTTGGT+GGG + chr1.3:49332737-49332756 None:intergenic 50.0%
CGATCTGGGTTGCAACATCA+AGG + chr1.3:49332431-49332450 None:intergenic 50.0%
CGGACCAGATCTTGTCTGAT+TGG + chr1.3:49331613-49331632 None:intergenic 50.0%
GATCAACACCTGCATCAGGA+TGG - chr1.3:49333294-49333313 MS.gene23959:CDS 50.0%
GGCTCCAATCAGACAAGATC+TGG - chr1.3:49331606-49331625 MS.gene23959:intron 50.0%
TCCACTCCCACCAAATGATC+CGG - chr1.3:49332728-49332747 MS.gene23959:intron 50.0%
TGATGGTTTCTCCCTCCTAC+AGG + chr1.3:49332566-49332585 None:intergenic 50.0%
TGTAGGAGGGAGAAACCATC+AGG - chr1.3:49332565-49332584 MS.gene23959:intron 50.0%
TTGCTACATGCACACCCGAT+AGG + chr1.3:49332644-49332663 None:intergenic 50.0%
! ATTTGGTGGGAGTGGAAGAG+CGG + chr1.3:49332724-49332743 None:intergenic 50.0%
! GATCTGATTTTCCCTGCACC+CGG + chr1.3:49332702-49332721 None:intergenic 50.0%
!! TTGTCGATTGGCCTGTAGGA+GGG - chr1.3:49332552-49332571 MS.gene23959:intron 50.0%
!! TTTGTCGATTGGCCTGTAGG+AGG - chr1.3:49332551-49332570 MS.gene23959:intron 50.0%
AATGTCAAGCACAAGGCGGC+TGG - chr1.3:49332591-49332610 MS.gene23959:intron 55.0%
ACACGCAGCGAAGAGATCAC+CGG + chr1.3:49331696-49331715 None:intergenic 55.0%
ACCTCCGGCGGTTACAAATG+CGG - chr1.3:49331578-49331597 MS.gene23959:intron 55.0%
ATTGAACCTTGCTCCCCCAC+CGG - chr1.3:49332680-49332699 MS.gene23959:intron 55.0%
CCTGGATCAACACCTGCATC+AGG - chr1.3:49333290-49333309 MS.gene23959:CDS 55.0%
CGAGCAGCAACCGGATCATT+TGG + chr1.3:49332741-49332760 None:intergenic 55.0%
CTTCTAACCGGATCTCGCAC+CGG + chr1.3:49331664-49331683 None:intergenic 55.0%
GAAGCAATCCGAGCAGCAAC+CGG + chr1.3:49332750-49332769 None:intergenic 55.0%
GATCCAGGAGTGGCTGATGA+GGG + chr1.3:49333278-49333297 None:intergenic 55.0%
GCAGCAACCGGATCATTTGG+TGG + chr1.3:49332738-49332757 None:intergenic 55.0%
GCGAAGAGATCACCGGAGTT+AGG + chr1.3:49331689-49331708 None:intergenic 55.0%
GCGGTTACAAATGCGGCGAA+TGG - chr1.3:49331585-49331604 MS.gene23959:intron 55.0%
GCTGCTCGGATTGCTTCTAC+CGG - chr1.3:49332753-49332772 MS.gene23959:intron 55.0%
TGATCCAGGAGTGGCTGATG+AGG + chr1.3:49333279-49333298 None:intergenic 55.0%
TTCGCCGCATTTGTAACCGC+CGG + chr1.3:49331585-49331604 None:intergenic 55.0%
! ACCGGATCATTTGGTGGGAG+TGG + chr1.3:49332732-49332751 None:intergenic 55.0%
! CTTTCAGCTGCTGGCCTATC+GGG - chr1.3:49332627-49332646 MS.gene23959:intron 55.0%
! GCTTTCAGCTGCTGGCCTAT+CGG - chr1.3:49332626-49332645 MS.gene23959:intron 55.0%
! GTTTTTCCCGGTCAGCGTGA+GGG - chr1.3:49331719-49331738 MS.gene23959:intron 55.0%
! TGATTTTCCCTGCACCCGGT+GGG + chr1.3:49332698-49332717 None:intergenic 55.0%
! TGTTTTTCCCGGTCAGCGTG+AGG - chr1.3:49331718-49331737 MS.gene23959:intron 55.0%
! TTGGATTGGGGTTTGCGGAG+AGG - chr1.3:49331819-49331838 MS.gene23959:intron 55.0%
!! CCTGATGCAGGTGTTGATCC+AGG + chr1.3:49333293-49333312 None:intergenic 55.0%
!! TGCAGGTGTTGATCCAGGAG+TGG + chr1.3:49333288-49333307 None:intergenic 55.0%
AACCGAACCCTCACGCTGAC+CGG + chr1.3:49331729-49331748 None:intergenic 60.0%
AAGGCTGCCCATCCTGATGC+AGG + chr1.3:49333305-49333324 None:intergenic 60.0%
CTTCCCTCATCAGCCACTCC+TGG - chr1.3:49333272-49333291 MS.gene23959:CDS 60.0%
GCTGGCATGCTTTCAGCTGC+TGG - chr1.3:49332618-49332637 MS.gene23959:intron 60.0%
TAACCGCCGGAGGTTGTTCG+CGG + chr1.3:49331572-49331591 None:intergenic 60.0%
TCGCGGCGGGATCTTGTAGA+CGG + chr1.3:49331555-49331574 None:intergenic 60.0%
TTGAACCTTGCTCCCCCACC+GGG - chr1.3:49332681-49332700 MS.gene23959:intron 60.0%
! ATTTTCCCTGCACCCGGTGG+GGG + chr1.3:49332696-49332715 None:intergenic 60.0%
! CTGATTTTCCCTGCACCCGG+TGG + chr1.3:49332699-49332718 None:intergenic 60.0%
! GATTTTCCCTGCACCCGGTG+GGG + chr1.3:49332697-49332716 None:intergenic 60.0%
! GCGTGAGGGTTCGGTTGAAC+CGG - chr1.3:49331733-49331752 MS.gene23959:intron 60.0%
! GTCTGGTGATGCTGATGCCG+CGG - chr1.3:49331913-49331932 MS.gene23959:intron 60.0%
ACCGAACCCTCACGCTGACC+GGG + chr1.3:49331728-49331747 None:intergenic 65.0%
AGATACGACGCGGAGACGGC+CGG + chr1.3:49331633-49331652 None:intergenic 65.0%
ATCCCGCCGCGAACAACCTC+CGG - chr1.3:49331563-49331582 MS.gene23959:intron 65.0%
GCCGCATTTGTAACCGCCGG+AGG + chr1.3:49331582-49331601 None:intergenic 65.0%
GCGTCGTATCTCGCCGTGAC+CGG - chr1.3:49331642-49331661 MS.gene23959:intron 65.0%
GGCGAGATACGACGCGGAGA+CGG + chr1.3:49331637-49331656 None:intergenic 65.0%
GGTCACGGCGAGATACGACG+CGG + chr1.3:49331643-49331662 None:intergenic 65.0%
GTCAAGCACAAGGCGGCTGG+TGG - chr1.3:49332594-49332613 MS.gene23959:intron 65.0%
TCCCGGTCAGCGTGAGGGTT+CGG - chr1.3:49331724-49331743 MS.gene23959:intron 65.0%
! ACCGGATCTCGCACCGGTCA+CGG + chr1.3:49331658-49331677 None:intergenic 65.0%
! CGCGAACGACGAGGAGTGTG+TGG + chr1.3:49331526-49331545 None:intergenic 65.0%
! TGGTGATGCTGATGCCGCGG+AGG - chr1.3:49331916-49331935 MS.gene23959:intron 65.0%
CACAAGGCGGCTGGTGGAGC+TGG - chr1.3:49332600-49332619 MS.gene23959:intron 70.0%
CGGCGACTTCGCGAACGACG+AGG + chr1.3:49331535-49331554 None:intergenic 70.0%
GCCGTGACCGGTGCGAGATC+CGG - chr1.3:49331654-49331673 MS.gene23959:intron 70.0%
TTGCTCCCCCACCGGGTGCA+GGG - chr1.3:49332688-49332707 MS.gene23959:intron 70.0%
CCGCCGCGAACAACCTCCGG+CGG - chr1.3:49331566-49331585 MS.gene23959:intron 75.0%
CCGCCGGAGGTTGTTCGCGG+CGG + chr1.3:49331569-49331588 None:intergenic 75.0%
CGCCGGAGGTTGTTCGCGGC+GGG + chr1.3:49331568-49331587 None:intergenic 75.0%
CTGCACCCGGTGGGGGAGCA+AGG + chr1.3:49332689-49332708 None:intergenic 75.0%
CTTGCTCCCCCACCGGGTGC+AGG - chr1.3:49332687-49332706 MS.gene23959:intron 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 49331491 49333328 49331491 ID=MS.gene23959
chr1.3 mRNA 49331491 49333328 49331491 ID=MS.gene23959.t1;Parent=MS.gene23959
chr1.3 exon 49332837 49333328 49332837 ID=MS.gene23959.t1.exon1;Parent=MS.gene23959.t1
chr1.3 CDS 49332837 49333328 49332837 ID=cds.MS.gene23959.t1;Parent=MS.gene23959.t1
chr1.3 exon 49331965 49332249 49331965 ID=MS.gene23959.t1.exon2;Parent=MS.gene23959.t1
chr1.3 CDS 49331965 49332249 49331965 ID=cds.MS.gene23959.t1;Parent=MS.gene23959.t1
chr1.3 exon 49331491 49331553 49331491 ID=MS.gene23959.t1.exon3;Parent=MS.gene23959.t1
chr1.3 CDS 49331491 49331553 49331491 ID=cds.MS.gene23959.t1;Parent=MS.gene23959.t1
Gene Sequence

>MS.gene23959

ATGTCGTTAGAAGAAGAAGAAGAGGGCTTCGACCACACACTCCTCGTCGTTCGCGAAGTCGCCGTCTACAAGATCCCGCCGCGAACAACCTCCGGCGGTTACAAATGCGGCGAATGGCTCCAATCAGACAAGATCTGGTCCGGCCGTCTCCGCGTCGTATCTCGCCGTGACCGGTGCGAGATCCGGTTAGAAGATCCTAACTCCGGTGATCTCTTCGCTGCGTGTTTTGTTTTTCCCGGTCAGCGTGAGGGTTCGGTTGAACCGGTTCTCGATTCGTCGAGATATTTTGTTCTGAAGATTGAAGATGGACAAGGGAAGCATGCGTTTATTGGATTGGGGTTTGCGGAGAGGAATGAAGCTTTTGATTTCAATGTTGCACTTTCTGATCATGAGAAGTATGTGAGAAGAGAACATGATAAGGAGTCTGGTGATGCTGATGCCGCGGAGGATTCACAGATCGATATTCATCCTGCTGTTAATCATAGGCTTAAGGTAGTTTCCATTAGCAAATTTGTGTGATCAATCTAATTCGCTTGATTATGCAGTTGAACTTTAATTTATTGTAGCGGTTATTGTTATTATGATGATTTGCTTACTATTTTCAGTTGAAAAATAGTTTATTTTTAGTATCATAAATGAAAGATTAGTTTTTTGTGTATTTTTGGTTAGTGTTAATTGTTGTGGATAAGTAGATCACTTAGATGCTTGTGTTATGATTACAATTGCTTTATGATATAATTATGATCATGGTGAATTGTCCGAGGTTATTTTTTGCCCAAATCCGATGTTTAGAGTAGTTTCTAGTTGTGTTTATGTTTCTCCAATGTTTTAAATCATGGTTGTGGTCGCATCATGATTGTTGCTATTGCAAATGATCGTGGGCAAATGGGGCCAATGTAATCTCAATAGTGACTGCAGAGACATCATATTTCATAAACCTTGATGTTGCAACCCAGATCGCAAACCCTTTTTTAAAACCATGTTTACAAATCACGGTCTTCTCTTAACAGTGTTTTTATATTTCTCATTGTGAATCCTTGCTTATCCTATGTTTGAAGTATTTGTCGATTGGCCTGTAGGAGGGAGAAACCATCAGGATTAATGTCAAGCACAAGGCGGCTGGTGGAGCTGGCATGCTTTCAGCTGCTGGCCTATCGGGTGTGCATGTAGCAACACCGAAGCCAAAAGTATTGAACCTTGCTCCCCCACCGGGTGCAGGGAAAATCAGATCCGCTCTTCCACTCCCACCAAATGATCCGGTTGCTGCTCGGATTGCTTCTACCGGTCCTGTATCTAGTGTTAAAGGGACAAAAGAAGGTGTGAAACAGTCTACTGATTCTCTATCGGATCTTTCTCAATTACAGGTATGCTGGTCCTGCTCATTCCTTAAGAGCCTTTCCTATTTCCACTTCATTTGTTTTTGCAATCAAATGTTTTAGAACTTGCAAATTATTGCATGGCTGGGCAACTACATTGTTTCTTTTAGACAGATTGAAGATTATTCTGCTAAATTTTATTTTGAAGATTCCGTGTACCTATCTAAGATTATGCTATGCTGCTAATTAAGTAACAGTCAACACTGAATAGCAAGAATACTAACTCCATTTCTTTGTTTATGCATATAAAATGACTGTTCTGATTTGTAAAACAACAGTTACATTGGTTATGCATATCCGTGATCATGTTACTTTGCCAGTTTAATTTCTCTATATTGTTGTAGTCCCAGACACTGTTTAATATGAATCATTTGTAACATTTCATGCTTGCATTTACAGAAAAATCTTCCCTCATCAGCCACTCCTGGATCAACACCTGCATCAGGATGGGCAGCCTTCTGA
Protein sequence

>MS.gene23959.t1

MSLEEEEEGFDHTLLVVREVAVYKIPPRTTSGGYKCGEWLQSDKIWSGRLRVVSRRDRCEIRLEDPNSGDLFAACFVFPGQREGSVEPVLDSSRYFVLKIEDGQGKHAFIGLGFAERNEAFDFNVALSDHEKYVRREHDKESGDADAAEDSQIDIHPAVNHRLKEGETIRINVKHKAAGGAGMLSAAGLSGVHVATPKPKVLNLAPPPGAGKIRSALPLPPNDPVAARIASTGPVSSVKGTKEGVKQSTDSLSDLSQLQKNLPSSATPGSTPASGWAAF