Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058545.t1 | XP_013449384.1 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 1.20E-59 | 238.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058545.t1 | P62772 | 93.7 | 111 | 7 | 0 | 2 | 112 | 1 | 111 | 2.2e-57 | 222.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058545.t1 | A2Q5X9 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 8.8e-60 | 238.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058545 | MS.gene23959 | 0.814395 | 1.57E-51 | -1.69E-46 |
| MS.gene058545 | MS.gene52274 | 0.987782 | 1.62E-171 | -1.69E-46 |
| MS.gene058545 | MS.gene89585 | 0.818801 | 1.62E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene058545 | MS.gene55486 | PPI |
| MS.gene55480 | MS.gene058545 | PPI |
| MS.gene47121 | MS.gene058545 | PPI |
| MS.gene074110 | MS.gene058545 | PPI |
| MS.gene058545 | MS.gene074111 | PPI |
| MS.gene47123 | MS.gene058545 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058545.t1 | MTR_7g080803 | 99.115 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 8.96e-82 | 234 |
| MS.gene058545.t1 | MTR_5g008460 | 88.496 | 113 | 13 | 0 | 1 | 113 | 1 | 113 | 2.16e-73 | 213 |
| MS.gene058545.t1 | MTR_7g080793 | 70.370 | 108 | 31 | 1 | 1 | 108 | 1 | 107 | 1.56e-51 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058545.t1 | AT4G10040 | 91.071 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 4.39e-75 | 218 |
| MS.gene058545.t1 | AT1G22840 | 89.286 | 112 | 12 | 0 | 1 | 112 | 1 | 112 | 2.07e-73 | 213 |
| MS.gene058545.t1 | AT1G22840 | 88.095 | 84 | 10 | 0 | 1 | 84 | 1 | 84 | 4.53e-52 | 159 |
Find 0 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATATTTACATAGTTTA+GGG | + | 50482:17704-17723 | MS.gene058545:intron | 10.0% |
| !! | TTATTATATTTACATAGTTT+AGG | + | 50482:17703-17722 | MS.gene058545:intron | 10.0% |
| !!! | ACAAATTTTTATAAACTTTA+AGG | - | 50482:17583-17602 | None:intergenic | 10.0% |
| !!! | CTTTTTTTATATTTATTGTA+AGG | + | 50482:17970-17989 | MS.gene058545:intron | 10.0% |
| !! | ATAAAAATGTCTATATCAAA+TGG | + | 50482:18588-18607 | MS.gene058545:intron | 15.0% |
| !!! | TCAATTTTTGAAATAACATT+GGG | - | 50482:18052-18071 | None:intergenic | 15.0% |
| !!! | TTCAATTTTTGAAATAACAT+TGG | - | 50482:18053-18072 | None:intergenic | 15.0% |
| !! | GTTAATTTATTATGGTTATG+TGG | + | 50482:18692-18711 | MS.gene058545:intron | 20.0% |
| !! | GTTATGCTGTTAATTTATTA+TGG | + | 50482:18684-18703 | MS.gene058545:intron | 20.0% |
| !! | TATGAAAATGTTGTTATACT+TGG | + | 50482:17778-17797 | MS.gene058545:intron | 20.0% |
| !!! | AACTCAAGGTATTATTATAT+AGG | + | 50482:18195-18214 | MS.gene058545:intron | 20.0% |
| !!! | CAATTTTTGAAATAACATTG+GGG | - | 50482:18051-18070 | None:intergenic | 20.0% |
| ! | AAATAAACTTAACAGCGATT+AGG | - | 50482:17298-17317 | None:intergenic | 25.0% |
| ! | GTAGCTTAACTATATGAATT+CGG | + | 50482:17547-17566 | MS.gene058545:intron | 25.0% |
| ! | TAGTTAAGCTACTAAATTGA+TGG | - | 50482:17539-17558 | None:intergenic | 25.0% |
| ! | TTACATAATGACTTTGTTCA+AGG | - | 50482:18345-18364 | None:intergenic | 25.0% |
| !! | TAGTGTTTTCTTCAATTAGT+AGG | + | 50482:18659-18678 | MS.gene058545:intron | 25.0% |
| !!! | ATGTTAGCTTTACCAAATTA+TGG | + | 50482:17447-17466 | MS.gene058545:intron | 25.0% |
| AAAACGAAAGAAAACAACAC+AGG | - | 50482:17242-17261 | None:intergenic | 30.0% | |
| AGTTGAATGAACATAACTCA+AGG | + | 50482:18181-18200 | MS.gene058545:intron | 30.0% | |
| ATTATGGTTATGTGGATGAT+AGG | + | 50482:18700-18719 | MS.gene058545:intron | 30.0% | |
| GATTGTTCAAGATCTTGTTA+GGG | + | 50482:17335-17354 | MS.gene058545:intron | 30.0% | |
| GTTGAAGCTTGAAATGTTTA+GGG | + | 50482:17388-17407 | MS.gene058545:intron | 30.0% | |
| GTTGTTATACTTGGTATGTT+CGG | + | 50482:17787-17806 | MS.gene058545:intron | 30.0% | |
| TGATTGTTCAAGATCTTGTT+AGG | + | 50482:17334-17353 | MS.gene058545:intron | 30.0% | |
| TGTTGAAGCTTGAAATGTTT+AGG | + | 50482:17387-17406 | MS.gene058545:intron | 30.0% | |
| TTATGTTCATTCAACTGACT+CGG | - | 50482:18177-18196 | None:intergenic | 30.0% | |
| ! | TGTTTTCTTCAATTAGTAGG+AGG | + | 50482:18662-18681 | MS.gene058545:intron | 30.0% |
| !! | TGTAGATCTGAGAGTTTTAT+GGG | - | 50482:17216-17235 | None:intergenic | 30.0% |
| AATGACTTTGTTCAAGGAGA+GGG | - | 50482:18339-18358 | None:intergenic | 35.0% | |
| ACGCGTATCAGTAAAATACT+TGG | - | 50482:18748-18767 | None:intergenic | 35.0% | |
| ATCAATGTGTTGTACCTTCT+TGG | - | 50482:18539-18558 | None:intergenic | 35.0% | |
| CCAAACAAACCATTCAGATT+GGG | - | 50482:18415-18434 | None:intergenic | 35.0% | |
| CTAAATTGCGTTGTCATTCA+CGG | + | 50482:17614-17633 | MS.gene058545:intron | 35.0% | |
| GAAATAACATTGGGGTGATA+TGG | - | 50482:18043-18062 | None:intergenic | 35.0% | |
| GATCGGACAAAACCATAATT+TGG | - | 50482:17462-17481 | None:intergenic | 35.0% | |
| GTTCAGCAAGTAATCATACA+AGG | - | 50482:18515-18534 | None:intergenic | 35.0% | |
| TAATGACTTTGTTCAAGGAG+AGG | - | 50482:18340-18359 | None:intergenic | 35.0% | |
| TCAATGTGTTGTACCTTCTT+GGG | - | 50482:18538-18557 | None:intergenic | 35.0% | |
| TCCAAACAAACCATTCAGAT+TGG | - | 50482:18416-18435 | None:intergenic | 35.0% | |
| ! | AGAAGGTACAACACATTGAT+TGG | + | 50482:18539-18558 | MS.gene058545:intron | 35.0% |
| ! | AGATTAGCATACCTTGTTTG+TGG | - | 50482:17183-17202 | None:intergenic | 35.0% |
| ! | ATCATTAGCTTCAACCAACT+AGG | - | 50482:17488-17507 | None:intergenic | 35.0% |
| ! | GGGTTTAAGATTCTATTGTG+TGG | + | 50482:17355-17374 | MS.gene058545:intron | 35.0% |
| ! | GTGTAGATCTGAGAGTTTTA+TGG | - | 50482:17217-17236 | None:intergenic | 35.0% |
| ! | TGTTTGGTTCAAAAGAGACA+AGG | + | 50482:17812-17831 | MS.gene058545:intron | 35.0% |
| !! | ATCTGAATGGTTTGTTTGGA+AGG | + | 50482:18416-18435 | MS.gene058545:CDS | 35.0% |
| !! | GTAGATCTGAGAGTTTTATG+GGG | - | 50482:17215-17234 | None:intergenic | 35.0% |
| !!! | TCGTTTTGTTTGAGATGAGA+GGG | + | 50482:17855-17874 | MS.gene058545:intron | 35.0% |
| !!! | TTCGTTTTGTTTGAGATGAG+AGG | + | 50482:17854-17873 | MS.gene058545:intron | 35.0% |
| AAGAGACAAGGAAAGGTTTC+TGG | + | 50482:17824-17843 | MS.gene058545:intron | 40.0% | |
| ATAAGATCAGCACGTTCTTG+AGG | - | 50482:18833-18852 | None:intergenic | 40.0% | |
| ATCTTAATGCAGTACATCCC+AGG | + | 50482:18776-18795 | MS.gene058545:intron | 40.0% | |
| CAATGTGTTGTACCTTCTTG+GGG | - | 50482:18537-18556 | None:intergenic | 40.0% | |
| CTTGGTATGTTCGGAATGTT+TGG | + | 50482:17796-17815 | MS.gene058545:intron | 40.0% | |
| CTTGTGAAGCAAACCAAAGA+GGG | - | 50482:17888-17907 | None:intergenic | 40.0% | |
| GAAAAACCATCTTTGTCCCT+GGG | - | 50482:18796-18815 | None:intergenic | 40.0% | |
| GACTCGGCTTAAAAACAAGA+AGG | - | 50482:18161-18180 | None:intergenic | 40.0% | |
| GGTTCAAAAGAGACAAGGAA+AGG | + | 50482:17817-17836 | MS.gene058545:intron | 40.0% | |
| TAGCAGCAGCAGAATAGAAA+AGG | - | 50482:18258-18277 | None:intergenic | 40.0% | |
| TCCTGGATATTCCTATTCTG+CGG | + | 50482:18453-18472 | MS.gene058545:CDS | 40.0% | |
| TCTTAATGCAGTACATCCCA+GGG | + | 50482:18777-18796 | MS.gene058545:intron | 40.0% | |
| TTAGCTTCAACCAACTAGGT+TGG | - | 50482:17484-17503 | None:intergenic | 40.0% | |
| TTGGTTGAACCAGACTTTCA+AGG | - | 50482:18729-18748 | None:intergenic | 40.0% | |
| TTGTGAAGCAAACCAAAGAG+GGG | - | 50482:17887-17906 | None:intergenic | 40.0% | |
| ! | CTTTATTTGTTGCCGACCTT+AGG | + | 50482:18388-18407 | MS.gene058545:intron | 40.0% |
| ! | TTCAACCAACTAGGTTGGAT+CGG | - | 50482:17479-17498 | None:intergenic | 40.0% |
| !! | CCCAATCTGAATGGTTTGTT+TGG | + | 50482:18412-18431 | MS.gene058545:CDS | 40.0% |
| !! | CTCTTTGGTTTGCTTCACAA+GGG | + | 50482:17887-17906 | MS.gene058545:intron | 40.0% |
| !! | TCTTTGGTTTGCTTCACAAG+GGG | + | 50482:17888-17907 | MS.gene058545:intron | 40.0% |
| ACAAGAACAAGGCTGTGGAT+TGG | + | 50482:18479-18498 | MS.gene058545:CDS | 45.0% | |
| ACCATTCAGATTGGGTCCTA+AGG | - | 50482:18407-18426 | None:intergenic | 45.0% | |
| ACCTTAGGACCCAATCTGAA+TGG | + | 50482:18403-18422 | MS.gene058545:intron | 45.0% | |
| CAAGAACAAGGCTGTGGATT+GGG | + | 50482:18480-18499 | MS.gene058545:CDS | 45.0% | |
| CCTTGTGAAGCAAACCAAAG+AGG | - | 50482:17889-17908 | None:intergenic | 45.0% | |
| GGAAAAACCATCTTTGTCCC+TGG | - | 50482:18797-18816 | None:intergenic | 45.0% | |
| GTTCTTGTTAGCCGCAGAAT+AGG | - | 50482:18467-18486 | None:intergenic | 45.0% | |
| TCTTGAGGCTTCTTAAGACC+AGG | - | 50482:18818-18837 | None:intergenic | 45.0% | |
| TTCAGATTGGGTCCTAAGGT+CGG | - | 50482:18403-18422 | None:intergenic | 45.0% | |
| TTCTGCGGCTAACAAGAACA+AGG | + | 50482:18468-18487 | MS.gene058545:CDS | 45.0% | |
| TTTGTCCGATCCAACCTAGT+TGG | + | 50482:17471-17490 | MS.gene058545:intron | 45.0% | |
| ! | GATAGGTAGCCTTGAAAGTC+TGG | + | 50482:18717-18736 | MS.gene058545:intron | 45.0% |
| ! | GGGACAAAGATGGTTTTTCC+TGG | + | 50482:18797-18816 | MS.gene058545:CDS | 45.0% |
| ! | GGTTTGTTTGGAAGGCAATC+TGG | + | 50482:18424-18443 | MS.gene058545:CDS | 45.0% |
| ! | TAGCATACCTTGTTTGTGGC+CGG | - | 50482:17179-17198 | None:intergenic | 45.0% |
| ! | TTACTTGCTGAACCCCAAGA+AGG | + | 50482:18522-18541 | MS.gene058545:CDS | 45.0% |
| ! | TTGTCATTCACGGATTGTGG+AGG | + | 50482:17624-17643 | MS.gene058545:intron | 45.0% |
| !! | CCTCTTTGGTTTGCTTCACA+AGG | + | 50482:17886-17905 | MS.gene058545:intron | 45.0% |
| !!! | GGTTTTGAAGATCTTCTCGC+CGG | - | 50482:17122-17141 | None:intergenic | 45.0% |
| AGAGGGTAAACACCCCTCTT+TGG | + | 50482:17872-17891 | MS.gene058545:intron | 50.0% | |
| GCCGCAGAATAGGAATATCC+AGG | - | 50482:18457-18476 | None:intergenic | 50.0% | |
| GGCTAACAAGAACAAGGCTG+TGG | + | 50482:18474-18493 | MS.gene058545:CDS | 50.0% | |
| GTACATCCCAGGGACAAAGA+TGG | + | 50482:18787-18806 | MS.gene058545:intron | 50.0% | |
| TCTTTCGATGAAGCTCCTCC+CGG | + | 50482:17085-17104 | MS.gene058545:CDS | 50.0% | |
| TTTGAAGATCTTCTCGCCGG+CGG | - | 50482:17119-17138 | None:intergenic | 50.0% | |
| ! | GCGTTGTCATTCACGGATTG+TGG | + | 50482:17621-17640 | MS.gene058545:intron | 50.0% |
| ! | TTTGTTCAAGGAGAGGGCAC+AGG | - | 50482:18333-18352 | None:intergenic | 50.0% |
| AGGCAATCTGGCACAACTCC+TGG | + | 50482:18436-18455 | MS.gene058545:CDS | 55.0% | |
| !! | GAACAAGGCTGTGGATTGGG+AGG | + | 50482:18483-18502 | MS.gene058545:CDS | 55.0% |
| CAGTGCCACACCGTCGACAA+AGG | + | 50482:17151-17170 | MS.gene058545:CDS | 60.0% | |
| !! | AAAGGCGCCGGCCACAAACA+AGG | + | 50482:17169-17188 | MS.gene058545:CDS | 60.0% |
| AAGATCTTCTCGCCGGCGGC+GGG | - | 50482:17115-17134 | None:intergenic | 65.0% | |
| GAAGATCTTCTCGCCGGCGG+CGG | - | 50482:17116-17135 | None:intergenic | 65.0% | |
| ! | CACACCGTCGACAAAGGCGC+CGG | + | 50482:17157-17176 | MS.gene058545:CDS | 65.0% |
| ! | GGTGTGGCACTGAGCGCACT+TGG | - | 50482:17143-17162 | None:intergenic | 65.0% |
| AGATCTTCTCGCCGGCGGCG+GGG | - | 50482:17114-17133 | None:intergenic | 70.0% | |
| CGGCGCCTTTGTCGACGGTG+TGG | - | 50482:17159-17178 | None:intergenic | 70.0% | |
| GTGGCCGGCGCCTTTGTCGA+CGG | - | 50482:17164-17183 | None:intergenic | 70.0% | |
| ! | CCTCCCGGAAACCCCGCCGC+CGG | + | 50482:17100-17119 | MS.gene058545:CDS | 80.0% |
| ! | CTCGCCGGCGGCGGGGTTTC+CGG | - | 50482:17107-17126 | None:intergenic | 80.0% |
| ! | TCGCCGGCGGCGGGGTTTCC+GGG | - | 50482:17106-17125 | None:intergenic | 80.0% |
| ! | CCGGCGGCGGGGTTTCCGGG+AGG | - | 50482:17103-17122 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50482 | gene | 17079 | 18883 | 17079 | ID=MS.gene058545 |
| 50482 | mRNA | 17079 | 18883 | 17079 | ID=MS.gene058545.t1;Parent=MS.gene058545 |
| 50482 | exon | 17079 | 17190 | 17079 | ID=MS.gene058545.t1.exon1;Parent=MS.gene058545.t1 |
| 50482 | CDS | 17079 | 17190 | 17079 | ID=cds.MS.gene058545.t1;Parent=MS.gene058545.t1 |
| 50482 | exon | 18410 | 18543 | 18410 | ID=MS.gene058545.t1.exon2;Parent=MS.gene058545.t1 |
| 50482 | CDS | 18410 | 18543 | 18410 | ID=cds.MS.gene058545.t1;Parent=MS.gene058545.t1 |
| 50482 | exon | 18788 | 18883 | 18788 | ID=MS.gene058545.t1.exon3;Parent=MS.gene058545.t1 |
| 50482 | CDS | 18788 | 18883 | 18788 | ID=cds.MS.gene058545.t1;Parent=MS.gene058545.t1 |
| Gene Sequence |
| Protein sequence |
