Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24077.t1 | KEH40270.1 | 98.5 | 267 | 4 | 0 | 14 | 280 | 196 | 462 | 2.00E-149 | 538.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24077.t1 | Q9SRE5 | 63.4 | 164 | 42 | 2 | 14 | 175 | 179 | 326 | 2.5e-47 | 190.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24077.t1 | A0A072VQ62 | 98.5 | 267 | 4 | 0 | 14 | 280 | 196 | 462 | 1.5e-149 | 538.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05537 | MS.gene24077 | 0.815733 | 7.95E-52 | -1.69E-46 |
| MS.gene057669 | MS.gene24077 | 0.836213 | 1.07E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24077.t1 | MTR_1g026560 | 97.770 | 269 | 6 | 0 | 13 | 281 | 195 | 463 | 0.0 | 557 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24077.t1 | AT1G76660 | 45.353 | 269 | 104 | 5 | 13 | 279 | 71 | 298 | 2.01e-62 | 201 |
| MS.gene24077.t1 | AT1G76660 | 45.353 | 269 | 104 | 5 | 13 | 279 | 178 | 405 | 2.80e-61 | 201 |
Find 0 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATATTTAAGGATAAAAT+TGG | - | 6092:29884-29903 | MS.gene24077:intergenic | 15.0% |
| !! | GAAGATATATTCTCAAAAAT+GGG | + | 6092:29997-30016 | MS.gene24077:CDS | 20.0% |
| !! | TGAAGATATATTCTCAAAAA+TGG | + | 6092:29996-30015 | MS.gene24077:CDS | 20.0% |
| !!! | TATGTTGTAGTTACTTTTAA+TGG | + | 6092:29728-29747 | MS.gene24077:intron | 20.0% |
| ! | ATTAAGTGTTTCAAAAGACT+CGG | + | 6092:29163-29182 | MS.gene24077:CDS | 25.0% |
| ! | GCAAGAAAGAAACATATTTA+AGG | - | 6092:29894-29913 | MS.gene24077:intergenic | 25.0% |
| ! | TATTACATCTTGAGAAATTG+TGG | + | 6092:29573-29592 | MS.gene24077:intron | 25.0% |
| ! | TTGAATTCTTTCATCAATGA+AGG | - | 6092:29407-29426 | MS.gene24077:intergenic | 25.0% |
| !!! | TACTCTTATTTTGAAAGAGA+GGG | - | 6092:29787-29806 | MS.gene24077:intergenic | 25.0% |
| AAAGTAACTACAACATACCA+AGG | - | 6092:29725-29744 | MS.gene24077:intergenic | 30.0% | |
| AATCAAATTCCTGTATGTGA+TGG | + | 6092:29506-29525 | MS.gene24077:CDS | 30.0% | |
| AATCTGCAGGAAATATCAAA+TGG | + | 6092:30029-30048 | MS.gene24077:CDS | 30.0% | |
| ATCTGCAGGAAATATCAAAT+GGG | + | 6092:30030-30049 | MS.gene24077:CDS | 30.0% | |
| CTGAAAATGGAAAATATCAG+AGG | + | 6092:29003-29022 | MS.gene24077:CDS | 30.0% | |
| TACCTATGCACAATTCTATT+TGG | + | 6092:29106-29125 | MS.gene24077:CDS | 30.0% | |
| TCCTTGATATTGGAGATATA+AGG | - | 6092:28911-28930 | MS.gene24077:intergenic | 30.0% | |
| ! | ATGAAGGTATGCTGTAAAAA+CGG | + | 6092:29531-29550 | MS.gene24077:intron | 30.0% |
| ! | CTCTGATATTTTCCATTTTC+AGG | - | 6092:29005-29024 | MS.gene24077:intergenic | 30.0% |
| !!! | CTACTCTTATTTTGAAAGAG+AGG | - | 6092:29788-29807 | MS.gene24077:intergenic | 30.0% |
| AATGGAAAATATCAGAGGAC+TGG | + | 6092:29008-29027 | MS.gene24077:CDS | 35.0% | |
| ATGGAAAATATCAGAGGACT+GGG | + | 6092:29009-29028 | MS.gene24077:CDS | 35.0% | |
| ATGTCAGATCATAAATCACC+GGG | + | 6092:29916-29935 | MS.gene24077:intron | 35.0% | |
| ATTTCTTCCACATCTTGCTT+AGG | - | 6092:29239-29258 | MS.gene24077:intergenic | 35.0% | |
| CATGTCAGATCATAAATCAC+CGG | + | 6092:29915-29934 | MS.gene24077:intron | 35.0% | |
| CGACACTAATGATGTTACTA+TGG | + | 6092:29052-29071 | MS.gene24077:CDS | 35.0% | |
| GCCTTATATCTCCAATATCA+AGG | + | 6092:28907-28926 | MS.gene24077:CDS | 35.0% | |
| GTCCAAATAGAATTGTGCAT+AGG | - | 6092:29111-29130 | MS.gene24077:intergenic | 35.0% | |
| TATTGGAGATATAAGGCTAC+TGG | - | 6092:28904-28923 | MS.gene24077:intergenic | 35.0% | |
| TCATCACTGTATCCTGAAAA+TGG | + | 6092:28990-29009 | MS.gene24077:CDS | 35.0% | |
| TCTGCAGGAAATATCAAATG+GGG | + | 6092:30031-30050 | MS.gene24077:CDS | 35.0% | |
| TGGAAGAAATAGAAGCTTAC+CGG | + | 6092:29249-29268 | MS.gene24077:CDS | 35.0% | |
| TTAATCTGCCACCATTATGA+GGG | - | 6092:29148-29167 | MS.gene24077:intergenic | 35.0% | |
| TTGATATTTCCTGCAGATTC+TGG | - | 6092:30028-30047 | MS.gene24077:intergenic | 35.0% | |
| ! | TCCATGAGAGTATGATTATC+AGG | - | 6092:29973-29992 | MS.gene24077:intergenic | 35.0% |
| ! | TTTGAAGTTGCTCTTCTCTA+TGG | - | 6092:29649-29668 | MS.gene24077:intergenic | 35.0% |
| AATCCTCCATTCCCTCATAA+TGG | + | 6092:29134-29153 | MS.gene24077:CDS | 40.0% | |
| AGAAGCTTGCGAGAAAGAAA+GGG | + | 6092:30092-30111 | MS.gene24077:CDS | 40.0% | |
| AGAATTGTGCATAGGTAGCA+GGG | - | 6092:29103-29122 | MS.gene24077:intergenic | 40.0% | |
| ATGCAGAGGTTGACTATAGA+AGG | + | 6092:30066-30085 | MS.gene24077:CDS | 40.0% | |
| ATTCAAAGCTCATGAGACTC+AGG | + | 6092:29421-29440 | MS.gene24077:CDS | 40.0% | |
| CATCTCAATAACTAGAGCCT+TGG | + | 6092:29705-29724 | MS.gene24077:intron | 40.0% | |
| CCTTCATATCCATCACATAC+AGG | - | 6092:29518-29537 | MS.gene24077:intergenic | 40.0% | |
| CGTGGAGATTTCTGATGTAA+TGG | + | 6092:29319-29338 | MS.gene24077:CDS | 40.0% | |
| CTTAATCTGCCACCATTATG+AGG | - | 6092:29149-29168 | MS.gene24077:intergenic | 40.0% | |
| GCCTGATAATCATACTCTCA+TGG | + | 6092:29969-29988 | MS.gene24077:CDS | 40.0% | |
| GGAAGAAATAGAAGCTTACC+GGG | + | 6092:29250-29269 | MS.gene24077:CDS | 40.0% | |
| TAGAAGTCCTAAGCAAGATG+TGG | + | 6092:29229-29248 | MS.gene24077:CDS | 40.0% | |
| TAGAATTGTGCATAGGTAGC+AGG | - | 6092:29104-29123 | MS.gene24077:intergenic | 40.0% | |
| TATAACCACCAGTCAATACG+TGG | + | 6092:29301-29320 | MS.gene24077:CDS | 40.0% | |
| TCACCAGAGTTCCTTGATAT+TGG | - | 6092:28921-28940 | MS.gene24077:intergenic | 40.0% | |
| TCTATAGTCAACCTCTGCAT+CGG | - | 6092:30066-30085 | MS.gene24077:intergenic | 40.0% | |
| TCTCCAATATCAAGGAACTC+TGG | + | 6092:28915-28934 | MS.gene24077:CDS | 40.0% | |
| TCTTGCTTAGGACTTCTAGA+AGG | - | 6092:29227-29246 | MS.gene24077:intergenic | 40.0% | |
| TGATGTTACTATGGCATCAC+AGG | + | 6092:29061-29080 | MS.gene24077:CDS | 40.0% | |
| TGCAGAGGTTGACTATAGAA+GGG | + | 6092:30067-30086 | MS.gene24077:CDS | 40.0% | |
| ! | AGAAATCTCCACGTATTGAC+TGG | - | 6092:29312-29331 | MS.gene24077:intergenic | 40.0% |
| ! | TATGAGGGAATGGAGGATTT+TGG | - | 6092:29133-29152 | MS.gene24077:intergenic | 40.0% |
| !! | ATTGATTTGCCAGAGGTCAA+GGG | - | 6092:29365-29384 | MS.gene24077:intergenic | 40.0% |
| !! | CCTGTATGTGATGGATATGA+AGG | + | 6092:29515-29534 | MS.gene24077:CDS | 40.0% |
| !! | TTCCATCATTGATTTGCCAG+AGG | - | 6092:29372-29391 | MS.gene24077:intergenic | 40.0% |
| AAAGCTCATGAGACTCAGGT+GGG | + | 6092:29425-29444 | MS.gene24077:CDS | 45.0% | |
| AAATATCAGAGGACTGGGTC+TGG | + | 6092:29014-29033 | MS.gene24077:CDS | 45.0% | |
| AATATCAGAGGACTGGGTCT+GGG | + | 6092:29015-29034 | MS.gene24077:CDS | 45.0% | |
| ACAGTGATGAATCCCACTGT+GGG | - | 6092:28980-28999 | MS.gene24077:intergenic | 45.0% | |
| ACTACATATTCGCTCTACCC+TGG | + | 6092:28870-28889 | MS.gene24077:CDS | 45.0% | |
| AGACTCGGATGCTCAATCTA+CGG | + | 6092:29178-29197 | MS.gene24077:CDS | 45.0% | |
| CAGAAGCTTGCGAGAAAGAA+AGG | + | 6092:30091-30110 | MS.gene24077:CDS | 45.0% | |
| CTCATCTGCACTGAAACCAA+AGG | - | 6092:29279-29298 | MS.gene24077:intergenic | 45.0% | |
| GACCTCTGGCAAATCAATGA+TGG | + | 6092:29367-29386 | MS.gene24077:CDS | 45.0% | |
| GAGCTGTTGCTACAATATCC+CGG | - | 6092:29937-29956 | MS.gene24077:intergenic | 45.0% | |
| GCAGAGGTTGACTATAGAAG+GGG | + | 6092:30068-30087 | MS.gene24077:CDS | 45.0% | |
| GGATATTGTAGCAACAGCTC+TGG | + | 6092:29937-29956 | MS.gene24077:CDS | 45.0% | |
| GTGGAAATGGACATCAGAGT+AGG | + | 6092:29201-29220 | MS.gene24077:CDS | 45.0% | |
| TACAGTGATGAATCCCACTG+TGG | - | 6092:28981-29000 | MS.gene24077:intergenic | 45.0% | |
| TTCTTTGCTTGCTTGCCAAC+TGG | - | 6092:29488-29507 | MS.gene24077:intergenic | 45.0% | |
| !! | AATCTCCACGTATTGACTGG+TGG | - | 6092:29309-29328 | MS.gene24077:intergenic | 45.0% |
| !! | CATTGATTTGCCAGAGGTCA+AGG | - | 6092:29366-29385 | MS.gene24077:intergenic | 45.0% |
| !!! | TTATTTTATAAAAGATAAAA+TGG | - | 6092:29847-29866 | MS.gene24077:intergenic | 5.0% |
| CAAAGCTCATGAGACTCAGG+TGG | + | 6092:29424-29443 | MS.gene24077:CDS | 50.0% | |
| CACTGAAACCAAAGGATGCC+CGG | - | 6092:29271-29290 | MS.gene24077:intergenic | 50.0% | |
| CCACCATTATGAGGGAATGG+AGG | - | 6092:29140-29159 | MS.gene24077:intergenic | 50.0% | |
| CCTCCATTCCCTCATAATGG+TGG | + | 6092:29137-29156 | MS.gene24077:CDS | 50.0% | |
| CTGCCACCATTATGAGGGAA+TGG | - | 6092:29143-29162 | MS.gene24077:intergenic | 50.0% | |
| GACTCGGATGCTCAATCTAC+GGG | + | 6092:29179-29198 | MS.gene24077:CDS | 50.0% | |
| GCTCAATCTACGGGTGGAAA+TGG | + | 6092:29188-29207 | MS.gene24077:CDS | 50.0% | |
| GGAGATATAAGGCTACTGGC+AGG | - | 6092:28900-28919 | MS.gene24077:intergenic | 50.0% | |
| TGGGATCATCCAGAATCTGC+AGG | + | 6092:30016-30035 | MS.gene24077:CDS | 50.0% | |
| TGTCAACCTCTTTCCCTGAG+CGG | + | 6092:28946-28965 | MS.gene24077:CDS | 50.0% | |
| AAACTCCCGCTCAGGGAAAG+AGG | - | 6092:28955-28974 | MS.gene24076:intergenic | 55.0% | |
| ACCATGATGCCCTTGACCTC+TGG | + | 6092:29353-29372 | MS.gene24077:CDS | 55.0% | |
| CGATTAGACGCGAGTCCAGT+TGG | + | 6092:29470-29489 | MS.gene24077:CDS | 55.0% | |
| CTGGACTCGCGTCTAATCGA+AGG | - | 6092:29469-29488 | MS.gene24077:intergenic | 55.0% | |
| GAAGCTTACCGGGCATCCTT+TGG | + | 6092:29260-29279 | MS.gene24077:CDS | 55.0% | |
| GGTTTCATGCTCCGATGCAG+AGG | + | 6092:30052-30071 | MS.gene24077:CDS | 55.0% | |
| GTCAACCTCTTTCCCTGAGC+GGG | + | 6092:28947-28966 | MS.gene24077:CDS | 55.0% | |
| TCGGATGCTCAATCTACGGG+TGG | + | 6092:29182-29201 | MS.gene24077:CDS | 55.0% | |
| ACTGGGTCTGGGAGGGTTTC+TGG | + | 6092:29026-29045 | MS.gene24077:CDS | 60.0% | |
| AGGCTACTGGCAGGACTTCC+AGG | - | 6092:28891-28910 | MS.gene24077:intergenic | 60.0% | |
| ATCAGAGGACTGGGTCTGGG+AGG | + | 6092:29018-29037 | MS.gene24077:CDS | 60.0% | |
| CTGGGTCTGGGAGGGTTTCT+GGG | + | 6092:29027-29046 | MS.gene24077:CDS | 60.0% | |
| GGCTACTGGCAGGACTTCCA+GGG | - | 6092:28890-28909 | MS.gene24077:intergenic | 60.0% | |
| TCAGAGGACTGGGTCTGGGA+GGG | + | 6092:29019-29038 | MS.gene24077:CDS | 60.0% | |
| !! | GCCAGAGGTCAAGGGCATCA+TGG | - | 6092:29357-29376 | MS.gene24077:intergenic | 60.0% |
| AGCGGGAGTTTCGCCCACAG+TGG | + | 6092:28964-28983 | MS.gene24077:CDS | 65.0% | |
| GCGGGAGTTTCGCCCACAGT+GGG | + | 6092:28965-28984 | MS.gene24077:CDS | 65.0% | |
| GTGGGCGAAACTCCCGCTCA+GGG | - | 6092:28962-28981 | MS.gene24077:intergenic | 65.0% | |
| TGTGGGCGAAACTCCCGCTC+AGG | - | 6092:28963-28982 | MS.gene24077:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6092 | gene | 28828 | 30131 | 28828 | ID=MS.gene24077 |
| 6092 | mRNA | 28828 | 30131 | 28828 | ID=MS.gene24077.t1;Parent=MS.gene24077 |
| 6092 | exon | 28828 | 29536 | 28828 | ID=MS.gene24077.t1.exon1;Parent=MS.gene24077.t1 |
| 6092 | CDS | 28828 | 29536 | 28828 | ID=cds.MS.gene24077.t1;Parent=MS.gene24077.t1 |
| 6092 | exon | 29923 | 30131 | 29923 | ID=MS.gene24077.t1.exon2;Parent=MS.gene24077.t1 |
| 6092 | CDS | 29923 | 30131 | 29923 | ID=cds.MS.gene24077.t1;Parent=MS.gene24077.t1 |
| Gene Sequence |
| Protein sequence |
