AlfalfaGEDB | MS.gene24393

M. sativa cultivar XinJiangDaYe / MS.gene24393

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13202.9 PF13405.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24393.t1	XP_003589208.1	94.7	95	1	1	1	91	1	95	1.10E-42	182.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24393.t1	G7I3M4	94.7	95	1	1	1	91	1	95	7.9e-43	182.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055701	MS.gene24393	0.822894	1.86E-53	-1.69E-46
MS.gene059609	MS.gene24393	0.817226	3.68E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24393.t1	MTR_1g019640	94.737	95	1	1	1	91	1	95	4.40e-61	181
MS.gene24393.t1	MTR_1g019660	89.474	95	6	1	1	91	1	95	3.89e-55	166
MS.gene24393.t1	MTR_1g019610	86.316	95	9	1	1	91	1	95	1.94e-54	164
MS.gene24393.t1	MTR_1g019670	65.591	93	29	1	1	90	1	93	1.47e-38	124
MS.gene24393.t1	MTR_1g019600	45.000	100	46	1	1	91	1	100	3.18e-19	75.5
MS.gene24393.t1	MTR_1g019590	43.678	87	45	1	5	91	21	103	2.55e-16	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTGATTAGTCCGTCTTTAT+TGG	0.241517	1.1:+3498743	None:intergenic
TATCTCCTTACCCGTGGTTT+TGG	0.273881	1.1:-3498693	MS.gene24393:CDS
TGATCCATAGACACCCTCTT+TGG	0.332546	1.1:+3498921	None:intergenic
TATCCCAAAGAGGGTGTCTA+TGG	0.383107	1.1:-3498925	MS.gene24393:CDS
GATCCATAGACACCCTCTTT+GGG	0.423736	1.1:+3498922	None:intergenic
CAACGATTGGCTCTCCATCC+AGG	0.437823	1.1:+3498783	None:intergenic
AGCTTTCATCAAACAACGAT+TGG	0.498009	1.1:+3498770	None:intergenic
TCTTGCCAAAACCACGGGTA+AGG	0.519368	1.1:+3498688	None:intergenic
CGATGGTCGTTACACAAAGA+AGG	0.521782	1.1:-3498844	MS.gene24393:CDS
TAGGTTCTTACTACCCTGGA+TGG	0.528156	1.1:-3498797	MS.gene24393:CDS
TTAGTTCTTGCCAAAACCAC+GGG	0.528157	1.1:+3498683	None:intergenic
TGGATCAAATCAACTTTGAA+AGG	0.536831	1.1:-3498905	MS.gene24393:CDS
GATTTAGGTTCTTACTACCC+TGG	0.543944	1.1:-3498801	MS.gene24393:CDS
AACGATTGGCTCTCCATCCA+GGG	0.580860	1.1:+3498784	None:intergenic
ATTAGTTCTTGCCAAAACCA+CGG	0.592102	1.1:+3498682	None:intergenic
GCCTGTGTTTATCCCAAAGA+GGG	0.602626	1.1:-3498934	MS.gene24393:CDS
ATTGATTATCTCCTTACCCG+TGG	0.619921	1.1:-3498699	MS.gene24393:CDS
AAAGCTGATGCCAATAAAGA+CGG	0.628000	1.1:-3498753	MS.gene24393:CDS
TGCCTGTGTTTATCCCAAAG+AGG	0.630941	1.1:-3498935	MS.gene24393:CDS
ACCCTCTTTGGGATAAACAC+AGG	0.645492	1.1:+3498933	None:intergenic
AGACGGACTAATCAGTGGCG+AGG	0.659853	1.1:-3498736	MS.gene24393:CDS
AATAAAGACGGACTAATCAG+TGG	0.668016	1.1:-3498741	MS.gene24393:CDS
AAGGCGGATATCAATAACGA+TGG	0.697636	1.1:-3498861	MS.gene24393:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGGAACATTATAGAAAAAT+TGG	-	chr1.1:3498733-3498752	MS.gene24393:CDS	20.0%
!	CATTATAGAAAAATTGGAGA+AGG	-	chr1.1:3498739-3498758	MS.gene24393:CDS	25.0%
!!!	AAAAAAGCTCTAAAGGATTT+AGG	-	chr1.1:3498803-3498822	MS.gene24393:CDS	25.0%
	TATAGAAAAATTGGAGAAGG+CGG	-	chr1.1:3498742-3498761	MS.gene24393:CDS	30.0%
	TGGATCAAATCAACTTTGAA+AGG	-	chr1.1:3498714-3498733	MS.gene24393:CDS	30.0%
	AAAGCTGATGCCAATAAAGA+CGG	-	chr1.1:3498866-3498885	MS.gene24393:CDS	35.0%
	AGCTTTCATCAAACAACGAT+TGG	+	chr1.1:3498852-3498871	None:intergenic	35.0%
!	ACTGATTAGTCCGTCTTTAT+TGG	+	chr1.1:3498879-3498898	None:intergenic	35.0%
!	GGAACTCAAAAAAGCTCTAA+AGG	-	chr1.1:3498796-3498815	MS.gene24393:CDS	35.0%
!!	AATAAAGACGGACTAATCAG+TGG	-	chr1.1:3498878-3498897	MS.gene24393:CDS	35.0%
	AAGGCGGATATCAATAACGA+TGG	-	chr1.1:3498758-3498777	MS.gene24393:CDS	40.0%
	ATTGATTATCTCCTTACCCG+TGG	-	chr1.1:3498920-3498939	MS.gene24393:CDS	40.0%
!	GATTTAGGTTCTTACTACCC+TGG	-	chr1.1:3498818-3498837	MS.gene24393:CDS	40.0%
!	TTAGTTCTTGCCAAAACCAC+GGG	+	chr1.1:3498939-3498958	None:intergenic	40.0%
	CGATGGTCGTTACACAAAGA+AGG	-	chr1.1:3498775-3498794	MS.gene24393:CDS	45.0%
	GATCCATAGACACCCTCTTT+GGG	+	chr1.1:3498700-3498719	None:intergenic	45.0%
	GCCTGTGTTTATCCCAAAGA+GGG	-	chr1.1:3498685-3498704	MS.gene24393:CDS	45.0%
	TAGGTTCTTACTACCCTGGA+TGG	-	chr1.1:3498822-3498841	MS.gene24393:CDS	45.0%
	TGATCCATAGACACCCTCTT+TGG	+	chr1.1:3498701-3498720	None:intergenic	45.0%
	TGCCTGTGTTTATCCCAAAG+AGG	-	chr1.1:3498684-3498703	MS.gene24393:CDS	45.0%
!	TATCTCCTTACCCGTGGTTT+TGG	-	chr1.1:3498926-3498945	MS.gene24393:CDS	45.0%
!!	ACCCTCTTTGGGATAAACAC+AGG	+	chr1.1:3498689-3498708	None:intergenic	45.0%
!!	TATCCCAAAGAGGGTGTCTA+TGG	-	chr1.1:3498694-3498713	MS.gene24393:CDS	45.0%
	TCTTGCCAAAACCACGGGTA+AGG	+	chr1.1:3498934-3498953	None:intergenic	50.0%
!	AACGATTGGCTCTCCATCCA+GGG	+	chr1.1:3498838-3498857	None:intergenic	50.0%
!	CAACGATTGGCTCTCCATCC+AGG	+	chr1.1:3498839-3498858	None:intergenic	55.0%
!!	AGACGGACTAATCAGTGGCG+AGG	-	chr1.1:3498883-3498902	MS.gene24393:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	3498683	3498958	3498683	ID=MS.gene24393
chr1.1	mRNA	3498683	3498958	3498683	ID=MS.gene24393.t1;Parent=MS.gene24393
chr1.1	exon	3498683	3498958	3498683	ID=MS.gene24393.t1.exon1;Parent=MS.gene24393.t1
chr1.1	CDS	3498683	3498958	3498683	ID=cds.MS.gene24393.t1;Parent=MS.gene24393.t1

Gene Sequence	>MS.gene24393 ATGCCTGTGTTTATCCCAAAGAGGGTGTCTATGGATCAAATCAACTTTGAAAGGAACATTATAGAAAAATTGGAGAAGGCGGATATCAATAACGATGGTCGTTACACAAAGAAGGAACTCAAAAAAGCTCTAAAGGATTTAGGTTCTTACTACCCTGGATGGAGAGCCAATCGTTGTTTGATGAAAGCTGATGCCAATAAAGACGGACTAATCAGTGGCGAGGAAATCGACACTTTAATTGATTATCTCCTTACCCGTGGTTTTGGCAAGAACTAA
Protein sequence	>MS.gene24393.t1 MPVFIPKRVSMDQINFERNIIEKLEKADINNDGRYTKKELKKALKDLGSYYPGWRANRCLMKADANKDGLISGEEIDTLIDYLLTRGFGKN