Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24662.t1 | AES92743.2 | 80.6 | 67 | 11 | 1 | 1 | 65 | 286 | 352 | 1.00E-21 | 112.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24662.t1 | A0A072UTI3 | 80.6 | 67 | 11 | 1 | 1 | 65 | 152 | 218 | 7.5e-22 | 112.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051710 | MS.gene24662 | 0.860407 | 2.11E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24662.t1 | MTR_4g133410 | 78.571 | 70 | 13 | 1 | 1 | 68 | 152 | 221 | 4.04e-31 | 109 |
| MS.gene24662.t1 | MTR_4g133410 | 80.597 | 67 | 11 | 1 | 1 | 65 | 286 | 352 | 6.75e-31 | 111 |
| MS.gene24662.t1 | MTR_4g133410 | 80.597 | 67 | 11 | 1 | 1 | 65 | 152 | 218 | 1.01e-30 | 110 |
| MS.gene24662.t1 | MTR_4g133410 | 80.597 | 67 | 11 | 1 | 1 | 65 | 286 | 352 | 1.66e-30 | 111 |
| MS.gene24662.t1 | MTR_8g070020 | 79.104 | 67 | 12 | 1 | 1 | 65 | 272 | 338 | 2.06e-30 | 110 |
| MS.gene24662.t1 | MTR_5g034890 | 78.462 | 65 | 13 | 1 | 1 | 65 | 333 | 396 | 2.50e-28 | 105 |
| MS.gene24662.t1 | MTR_7g007510 | 40.870 | 115 | 13 | 2 | 1 | 60 | 425 | 539 | 1.09e-16 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGGTTGACGGTTGCCTC+TGG | 0.328039 | 7.2:+48743051 | MS.gene24662:CDS |
| AAGTTGGATATGCATGAATC+AGG | 0.385598 | 7.2:-48742769 | None:intergenic |
| AGGAGTTGAGTGGTCAAGAT+TGG | 0.392822 | 7.2:+48743033 | MS.gene24662:CDS |
| GGAGTTGAGTGGTCAAGATT+GGG | 0.433630 | 7.2:+48743034 | MS.gene24662:CDS |
| GAGGTTGCTTCTCCTACTTA+AGG | 0.448142 | 7.2:+48743013 | MS.gene24662:CDS |
| AGTGGTCAAGATTGGGTTGA+CGG | 0.460395 | 7.2:+48743041 | MS.gene24662:CDS |
| TATGAAGATATGGAACAATC+TGG | 0.474665 | 7.2:+48742716 | None:intergenic |
| ACGGTTGCCTCTGGGCCATC+CGG | 0.483450 | 7.2:+48743060 | MS.gene24662:CDS |
| TTGGGTTGACGGTTGCCTCT+GGG | 0.527030 | 7.2:+48743052 | MS.gene24662:CDS |
| ACATCAAATTCACCGCAAGT+TGG | 0.541945 | 7.2:-48742785 | None:intergenic |
| GATGTGTTATGTAATTTACA+TGG | 0.543048 | 7.2:+48742981 | MS.gene24662:CDS |
| ATTTACATGGCGAGTTGGAG+AGG | 0.585953 | 7.2:+48742994 | MS.gene24662:CDS |
| AGTTGGATATGCATGAATCA+GGG | 0.599687 | 7.2:-48742768 | None:intergenic |
| ATGTAATTTACATGGCGAGT+TGG | 0.602494 | 7.2:+48742989 | MS.gene24662:CDS |
| GACCACTCAACTCCTTAAGT+AGG | 0.607816 | 7.2:-48743025 | None:intergenic |
| ATTCATGCATATCCAACTTG+CGG | 0.638699 | 7.2:+48742773 | MS.gene24662:CDS |
| CTCCTACTTAAGGAGTTGAG+TGG | 0.649124 | 7.2:+48743023 | MS.gene24662:CDS |
| CTAAATACCGGATGGCCCAG+AGG | 0.695432 | 7.2:-48743067 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAATTATAAGAATTAAATTT+TGG | + | chr7.2:48743271-48743290 | MS.gene24662:intron | 10.0% |
| !! | TAGTTAAACACAAAAATTTA+AGG | + | chr7.2:48743087-48743106 | MS.gene24662:intron | 15.0% |
| !!! | TTTATGTTCATTGTATATTT+TGG | + | chr7.2:48743162-48743181 | MS.gene24662:intron | 15.0% |
| !! | ATACAATGAACATAAACAAA+TGG | - | chr7.2:48743158-48743177 | None:intergenic | 20.0% |
| !! | TGAATTTGATGTTAAAAAAC+AGG | + | chr7.2:48742796-48742815 | MS.gene24662:CDS | 20.0% |
| !!! | TTTTGTGTTTAACTAAATAC+CGG | - | chr7.2:48743082-48743101 | None:intergenic | 20.0% |
| ! | ATCAATCATTGTACATTCTT+AGG | + | chr7.2:48742959-48742978 | MS.gene24662:intron | 25.0% |
| ! | GAATCATTATTCAAGTCATA+AGG | - | chr7.2:48743250-48743269 | None:intergenic | 25.0% |
| ! | GATGTGTTATGTAATTTACA+TGG | + | chr7.2:48742981-48743000 | MS.gene24662:CDS | 25.0% |
| !! | ACAATGATTGATGAGTTTTA+AGG | - | chr7.2:48742951-48742970 | None:intergenic | 25.0% |
| !! | CACAAAAATTTAAGGTATGA+CGG | + | chr7.2:48743095-48743114 | MS.gene24662:intron | 25.0% |
| !!! | ATGAGTTTTAAGGTTGAAAA+TGG | - | chr7.2:48742941-48742960 | None:intergenic | 25.0% |
| ! | CAAAAACTTCTTTTGTCAAG+TGG | - | chr7.2:48742745-48742764 | None:intergenic | 30.0% |
| ! | CTCTTGTTTAAACAGTTGAA+TGG | - | chr7.2:48743195-48743214 | None:intergenic | 30.0% |
| ! | CTTTCTACAGTTTCTCTTTT+GGG | + | chr7.2:48742841-48742860 | MS.gene24662:intron | 30.0% |
| ! | TATTGGTCTCATGTATGTAT+AGG | + | chr7.2:48743325-48743344 | MS.gene24662:intron | 30.0% |
| ! | TCTTTCTACAGTTTCTCTTT+TGG | + | chr7.2:48742840-48742859 | MS.gene24662:intron | 30.0% |
| ! | TTTCTACAGTTTCTCTTTTG+GGG | + | chr7.2:48742842-48742861 | MS.gene24662:intron | 30.0% |
| !! | GAGAGATATGCTTTTTGATA+AGG | + | chr7.2:48743212-48743231 | MS.gene24662:intron | 30.0% |
| !!! | GTTTTAAGGTTGAAAATGGA+AGG | - | chr7.2:48742937-48742956 | None:intergenic | 30.0% |
| AAGTTGGATATGCATGAATC+AGG | - | chr7.2:48742772-48742791 | None:intergenic | 35.0% | |
| AATATGCCATGTCCTTTACA+AGG | - | chr7.2:48742871-48742890 | None:intergenic | 35.0% | |
| AGTTGGATATGCATGAATCA+GGG | - | chr7.2:48742771-48742790 | None:intergenic | 35.0% | |
| ATATTGTCAGTCTCATGGTA+GGG | + | chr7.2:48742886-48742905 | MS.gene24662:intron | 35.0% | |
| ATGTAATTTACATGGCGAGT+TGG | + | chr7.2:48742989-48743008 | MS.gene24662:CDS | 35.0% | |
| ATTCATGCATATCCAACTTG+CGG | + | chr7.2:48742773-48742792 | MS.gene24662:CDS | 35.0% | |
| GTGTATGAAGCATGACATAT+TGG | + | chr7.2:48743308-48743327 | MS.gene24662:intron | 35.0% | |
| GTGTTTAACTAAATACCGGA+TGG | - | chr7.2:48743078-48743097 | None:intergenic | 35.0% | |
| ACATCAAATTCACCGCAAGT+TGG | - | chr7.2:48742788-48742807 | None:intergenic | 40.0% | |
| ATGGCATATTGTCAGTCTCA+TGG | + | chr7.2:48742881-48742900 | MS.gene24662:intron | 40.0% | |
| CATATTGTCAGTCTCATGGT+AGG | + | chr7.2:48742885-48742904 | MS.gene24662:intron | 40.0% | |
| AGGAGTTGAGTGGTCAAGAT+TGG | + | chr7.2:48743033-48743052 | MS.gene24662:CDS | 45.0% | |
| CTCCTACTTAAGGAGTTGAG+TGG | + | chr7.2:48743023-48743042 | MS.gene24662:CDS | 45.0% | |
| GACCACTCAACTCCTTAAGT+AGG | - | chr7.2:48743028-48743047 | None:intergenic | 45.0% | |
| GAGGTTGCTTCTCCTACTTA+AGG | + | chr7.2:48743013-48743032 | MS.gene24662:CDS | 45.0% | |
| GGAGTTGAGTGGTCAAGATT+GGG | + | chr7.2:48743034-48743053 | MS.gene24662:CDS | 45.0% | |
| ! | ATTTACATGGCGAGTTGGAG+AGG | + | chr7.2:48742994-48743013 | MS.gene24662:CDS | 45.0% |
| !! | AGTGGTCAAGATTGGGTTGA+CGG | + | chr7.2:48743041-48743060 | MS.gene24662:CDS | 45.0% |
| !!! | CTTTTGGGGTGACCTTGTAA+AGG | + | chr7.2:48742856-48742875 | MS.gene24662:intron | 45.0% |
| !! | ATAAAATCATAATTTAATAT+TGG | - | chr7.2:48743131-48743150 | None:intergenic | 5.0% |
| !! | TAAAATCATAATTTAATATT+GGG | - | chr7.2:48743130-48743149 | None:intergenic | 5.0% |
| GGGTGACCTTGTAAAGGACA+TGG | + | chr7.2:48742862-48742881 | MS.gene24662:intron | 50.0% | |
| CTAAATACCGGATGGCCCAG+AGG | - | chr7.2:48743070-48743089 | None:intergenic | 55.0% | |
| !! | ATTGGGTTGACGGTTGCCTC+TGG | + | chr7.2:48743051-48743070 | MS.gene24662:CDS | 55.0% |
| !! | TTGGGTTGACGGTTGCCTCT+GGG | + | chr7.2:48743052-48743071 | MS.gene24662:CDS | 55.0% |
| ACGGTTGCCTCTGGGCCATC+CGG | + | chr7.2:48743060-48743079 | MS.gene24662:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 48742725 | 48743359 | 48742725 | ID=MS.gene24662 |
| chr7.2 | mRNA | 48742725 | 48743359 | 48742725 | ID=MS.gene24662.t1;Parent=MS.gene24662 |
| chr7.2 | exon | 48742725 | 48742817 | 48742725 | ID=MS.gene24662.t1.exon1;Parent=MS.gene24662.t1 |
| chr7.2 | CDS | 48742725 | 48742817 | 48742725 | ID=cds.MS.gene24662.t1;Parent=MS.gene24662.t1 |
| chr7.2 | exon | 48742981 | 48743081 | 48742981 | ID=MS.gene24662.t1.exon2;Parent=MS.gene24662.t1 |
| chr7.2 | CDS | 48742981 | 48743081 | 48742981 | ID=cds.MS.gene24662.t1;Parent=MS.gene24662.t1 |
| chr7.2 | exon | 48743347 | 48743359 | 48743347 | ID=MS.gene24662.t1.exon3;Parent=MS.gene24662.t1 |
| chr7.2 | CDS | 48743347 | 48743359 | 48743347 | ID=cds.MS.gene24662.t1;Parent=MS.gene24662.t1 |
| Gene Sequence |
| Protein sequence |
