Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24683.t1 | XP_013450110.1 | 87.9 | 289 | 33 | 1 | 1 | 287 | 1 | 289 | 7.30E-109 | 403.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24683.t1 | A0A072U4S0 | 87.9 | 289 | 33 | 1 | 1 | 287 | 1 | 289 | 5.2e-109 | 403.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050309 | MS.gene24683 | 0.800167 | 1.64E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24683.t1 | MTR_7g104725 | 97.232 | 289 | 6 | 1 | 1 | 287 | 1 | 289 | 0.0 | 565 |
| MS.gene24683.t1 | MTR_1g059890 | 44.481 | 308 | 118 | 14 | 1 | 285 | 3 | 280 | 2.58e-61 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24683.t1 | AT3G52710 | 41.391 | 302 | 134 | 12 | 5 | 279 | 3 | 288 | 1.17e-49 | 166 |
| MS.gene24683.t1 | AT2G36220 | 40.989 | 283 | 128 | 12 | 2 | 275 | 4 | 256 | 3.69e-41 | 143 |
Find 74 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCGAAGTCGAATTGGAATT+AGG | 0.233462 | 7.3:-9776699 | None:intergenic |
| ATTCTCCGCCGGAATCATTC+TGG | 0.318910 | 7.3:+9776580 | MS.gene24683:CDS |
| AAGGAAGGTTTCTGAGGTTT+AGG | 0.324775 | 7.3:-9776777 | None:intergenic |
| GAGGGTGAACCGCCTGGTTT+GGG | 0.327472 | 7.3:+9777218 | MS.gene24683:CDS |
| TCGAAGTCGAATTGGAATTA+GGG | 0.329363 | 7.3:-9776698 | None:intergenic |
| GGAGGGTGAACCGCCTGGTT+TGG | 0.332813 | 7.3:+9777217 | MS.gene24683:CDS |
| CGACGTGTCGTTTGTGGAAT+CGG | 0.337131 | 7.3:+9777175 | MS.gene24683:CDS |
| CTTAAACGACGTGTCGTTTG+TGG | 0.368356 | 7.3:+9777169 | MS.gene24683:CDS |
| GGCTTTGGCTGCGGCGGTGA+CGG | 0.374114 | 7.3:-9776949 | None:intergenic |
| AATGATTCCGGCGGAGAATC+GGG | 0.385576 | 7.3:-9776576 | None:intergenic |
| AAATCGAAGACGTCGATGAT+TGG | 0.397400 | 7.3:+9776750 | MS.gene24683:CDS |
| CTGGAGAGCCAGAATGATTC+CGG | 0.399676 | 7.3:-9776588 | None:intergenic |
| CCAACGGAGGCGCTTCGCTT+TGG | 0.403150 | 7.3:-9776849 | None:intergenic |
| TGTTGAACTTGAATTTCCTT+TGG | 0.404679 | 7.3:-9776667 | None:intergenic |
| GGGATGATGCGGTTCACGTC+TGG | 0.406943 | 7.3:+9777242 | MS.gene24683:CDS |
| TTTCCCATGCAAGACACTTT+CGG | 0.424095 | 7.3:-9776897 | None:intergenic |
| GAATGATTCCGGCGGAGAAT+CGG | 0.431837 | 7.3:-9776577 | None:intergenic |
| GTTGGAAGTCGAAGTCGAAT+TGG | 0.435363 | 7.3:-9776706 | None:intergenic |
| GGATGATGATGAGAGGATTC+CGG | 0.444774 | 7.3:-9776555 | None:intergenic |
| GGCTGCGGCGGTGACGGAGG+AGG | 0.447604 | 7.3:-9776943 | None:intergenic |
| CAAGACACTTTCGGTGATGA+AGG | 0.466166 | 7.3:-9776888 | None:intergenic |
| ATCAGTTGAACCGCCGGCAC+AGG | 0.471514 | 7.3:-9776481 | None:intergenic |
| GAGAAACGATTCTTCGGCGT+TGG | 0.475237 | 7.3:+9777079 | MS.gene24683:CDS |
| CACGGCGGCTTTGGCTGCGG+CGG | 0.478127 | 7.3:-9776955 | None:intergenic |
| ATCATGTTTATTCCTTCCAC+CGG | 0.487247 | 7.3:-9777054 | None:intergenic |
| GGTGAACCGCCTGGTTTGGG+TGG | 0.490276 | 7.3:+9777221 | MS.gene24683:CDS |
| TTTCACGGCGGCTTTGGCTG+CGG | 0.490475 | 7.3:-9776958 | None:intergenic |
| CGCATCATCCCACCCAAACC+AGG | 0.492634 | 7.3:-9777230 | None:intergenic |
| GGAAACAACTTAATGTTACT+CGG | 0.496570 | 7.3:-9776828 | None:intergenic |
| CAAAGCGAAGCGCCTCCGTT+GGG | 0.503793 | 7.3:+9776850 | MS.gene24683:CDS |
| CTGGAAGACGGTCTGAGTCG+TGG | 0.507701 | 7.3:+9777261 | MS.gene24683:CDS |
| CCAAAGCGAAGCGCCTCCGT+TGG | 0.522856 | 7.3:+9776849 | MS.gene24683:CDS |
| GTATCGGAGGGTGAACCGCC+TGG | 0.524644 | 7.3:+9777212 | MS.gene24683:CDS |
| GTGAACCGCCTGGTTTGGGT+GGG | 0.526851 | 7.3:+9777222 | MS.gene24683:CDS |
| ATTTGCGAATCGCTGAGAGT+TGG | 0.530028 | 7.3:-9776724 | None:intergenic |
| TGATCGGAGAAACGATTCTT+CGG | 0.530088 | 7.3:+9777073 | MS.gene24683:CDS |
| TGGAAGACGGTCTGAGTCGT+GGG | 0.533984 | 7.3:+9777262 | MS.gene24683:CDS |
| AGATGAAGAAAACGAGTGGT+TGG | 0.534269 | 7.3:+9776611 | MS.gene24683:CDS |
| ACACTGGTTTCAGCCTGTGC+CGG | 0.536079 | 7.3:+9776468 | MS.gene24683:CDS |
| TGATGAGAGGATTCCGGTGG+AGG | 0.536818 | 7.3:-9776549 | None:intergenic |
| TTTGGCTGCGGCGGTGACGG+AGG | 0.537701 | 7.3:-9776946 | None:intergenic |
| AGATTCTTAGCGTCGAAGGA+AGG | 0.541094 | 7.3:-9776792 | None:intergenic |
| TCTCAAAACGGATCCTCCAC+CGG | 0.544913 | 7.3:+9776536 | MS.gene24683:CDS |
| ATCGTCGGCTTGTGTCTCGC+AGG | 0.552782 | 7.3:-9776514 | None:intergenic |
| GTGCCGGCGGTTCAACTGAT+CGG | 0.557681 | 7.3:+9776484 | MS.gene24683:CDS |
| CTGGTTTGGGTGGGATGATG+CGG | 0.557941 | 7.3:+9777231 | MS.gene24683:CDS |
| CTGGTTTCAGCCTGTGCCGG+CGG | 0.561067 | 7.3:+9776471 | MS.gene24683:CDS |
| TTCAAAAGCGCGTGATAGCA+CGG | 0.567098 | 7.3:+9777142 | MS.gene24683:CDS |
| TCATCATCCCGATTCTCCGC+CGG | 0.570450 | 7.3:+9776569 | MS.gene24683:CDS |
| TTTCCGATCAGTTGAACCGC+CGG | 0.577458 | 7.3:-9776487 | None:intergenic |
| TCTCAAGGATATGCTACAAG+TGG | 0.579670 | 7.3:+9776443 | None:intergenic |
| ATCACCGAAAGTGTCTTGCA+TGG | 0.582694 | 7.3:+9776893 | MS.gene24683:CDS |
| ACAAGCCGACGATCTCAAAA+CGG | 0.592406 | 7.3:+9776524 | MS.gene24683:CDS |
| TCACCGAAAGTGTCTTGCAT+GGG | 0.596284 | 7.3:+9776894 | MS.gene24683:CDS |
| ACGAAGATTCTTAGCGTCGA+AGG | 0.596920 | 7.3:-9776796 | None:intergenic |
| GACGAGAAACAGCGTATCGG+AGG | 0.603677 | 7.3:+9777199 | MS.gene24683:CDS |
| GCGTCGAAGGAAGGTTTCTG+AGG | 0.608308 | 7.3:-9776783 | None:intergenic |
| GTGGAAGGAATAAACATGAT+CGG | 0.609068 | 7.3:+9777057 | MS.gene24683:CDS |
| TGCGGTTCACGTCTGGAAGA+CGG | 0.620278 | 7.3:+9777249 | MS.gene24683:CDS |
| GTTTATTCCTTCCACCGGAG+CGG | 0.629764 | 7.3:-9777049 | None:intergenic |
| TATGCTACAAGTGGAAACAC+TGG | 0.636979 | 7.3:+9776452 | None:intergenic |
| ACGAATACTATCTTGACACG+TGG | 0.641215 | 7.3:-9777301 | None:intergenic |
| AACAAACGATTTCCCAACGG+AGG | 0.643622 | 7.3:-9776862 | None:intergenic |
| AAGACGGTCTGAGTCGTGGG+TGG | 0.657734 | 7.3:+9777265 | MS.gene24683:CDS |
| TGATGATGAGAGGATTCCGG+TGG | 0.659739 | 7.3:-9776552 | None:intergenic |
| GGTGACGAGAAACAGCGTAT+CGG | 0.661202 | 7.3:+9777196 | MS.gene24683:CDS |
| GAACGCAAAGAATCAACCAA+AGG | 0.662016 | 7.3:+9776651 | MS.gene24683:CDS |
| AGAATCGGGATGATGATGAG+AGG | 0.662087 | 7.3:-9776562 | None:intergenic |
| TTCAACAAACGATTTCCCAA+CGG | 0.663139 | 7.3:-9776865 | None:intergenic |
| ACGAGAAACAGCGTATCGGA+GGG | 0.675020 | 7.3:+9777200 | MS.gene24683:CDS |
| CCAGAGATGAAGAAAACGAG+TGG | 0.676074 | 7.3:+9776607 | MS.gene24683:CDS |
| GAGAGCCAGAATGATTCCGG+CGG | 0.678279 | 7.3:-9776585 | None:intergenic |
| ATCATCCCACCCAAACCAGG+CGG | 0.701016 | 7.3:-9777227 | None:intergenic |
| GGAAAAGTCAGATCTAAACG+CGG | 0.786700 | 7.3:+9776915 | MS.gene24683:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GGAAACAACTTAATGTTACT+CGG | - | chr7.3:9776831-9776850 | None:intergenic | 30.0% | |
| TGTTGAACTTGAATTTCCTT+TGG | - | chr7.3:9776670-9776689 | None:intergenic | 30.0% | |
| !!! | GTGTTTTGATTGTTTTCTCT+CGG | - | chr7.3:9777000-9777019 | None:intergenic | 30.0% |
| ATCATGTTTATTCCTTCCAC+CGG | - | chr7.3:9777057-9777076 | None:intergenic | 35.0% | |
| GTGGAAGGAATAAACATGAT+CGG | + | chr7.3:9777057-9777076 | MS.gene24683:CDS | 35.0% | |
| TCGAAGTCGAATTGGAATTA+GGG | - | chr7.3:9776701-9776720 | None:intergenic | 35.0% | |
| TTCAACAAACGATTTCCCAA+CGG | - | chr7.3:9776868-9776887 | None:intergenic | 35.0% | |
| AAATCGAAGACGTCGATGAT+TGG | + | chr7.3:9776750-9776769 | MS.gene24683:CDS | 40.0% | |
| AGATGAAGAAAACGAGTGGT+TGG | + | chr7.3:9776611-9776630 | MS.gene24683:CDS | 40.0% | |
| GAACGCAAAGAATCAACCAA+AGG | + | chr7.3:9776651-9776670 | MS.gene24683:CDS | 40.0% | |
| GGAAAAGTCAGATCTAAACG+CGG | + | chr7.3:9776915-9776934 | MS.gene24683:CDS | 40.0% | |
| GTCGAAGTCGAATTGGAATT+AGG | - | chr7.3:9776702-9776721 | None:intergenic | 40.0% | |
| TGATCGGAGAAACGATTCTT+CGG | + | chr7.3:9777073-9777092 | MS.gene24683:CDS | 40.0% | |
| TTTCCCATGCAAGACACTTT+CGG | - | chr7.3:9776900-9776919 | None:intergenic | 40.0% | |
| !! | AAGGAAGGTTTCTGAGGTTT+AGG | - | chr7.3:9776780-9776799 | None:intergenic | 40.0% |
| !!! | TCTCGCCGTTTTTTCTTTCA+CGG | - | chr7.3:9776976-9776995 | None:intergenic | 40.0% |
| AACAAACGATTTCCCAACGG+AGG | - | chr7.3:9776865-9776884 | None:intergenic | 45.0% | |
| ACAAGCCGACGATCTCAAAA+CGG | + | chr7.3:9776524-9776543 | MS.gene24683:CDS | 45.0% | |
| AGCCGCCGTGAAAGAAAAAA+CGG | + | chr7.3:9776968-9776987 | MS.gene24683:CDS | 45.0% | |
| ATTTGCGAATCGCTGAGAGT+TGG | - | chr7.3:9776727-9776746 | None:intergenic | 45.0% | |
| CAAGACACTTTCGGTGATGA+AGG | - | chr7.3:9776891-9776910 | None:intergenic | 45.0% | |
| CCAGAGATGAAGAAAACGAG+TGG | + | chr7.3:9776607-9776626 | MS.gene24683:CDS | 45.0% | |
| GTTGGAAGTCGAAGTCGAAT+TGG | - | chr7.3:9776709-9776728 | None:intergenic | 45.0% | |
| ! | ACGAAGATTCTTAGCGTCGA+AGG | - | chr7.3:9776799-9776818 | None:intergenic | 45.0% |
| ! | AGAATCGGGATGATGATGAG+AGG | - | chr7.3:9776565-9776584 | None:intergenic | 45.0% |
| ! | AGATTCTTAGCGTCGAAGGA+AGG | - | chr7.3:9776795-9776814 | None:intergenic | 45.0% |
| ! | CCACTCGTTTTCTTCATCTC+TGG | - | chr7.3:9776610-9776629 | None:intergenic | 45.0% |
| ! | GGATGATGATGAGAGGATTC+CGG | - | chr7.3:9776558-9776577 | None:intergenic | 45.0% |
| ! | TTCAAAAGCGCGTGATAGCA+CGG | + | chr7.3:9777142-9777161 | MS.gene24683:CDS | 45.0% |
| ! | TTTTTCTTTCACGGCGGCTT+TGG | - | chr7.3:9776967-9776986 | None:intergenic | 45.0% |
| !! | ATCACCGAAAGTGTCTTGCA+TGG | + | chr7.3:9776893-9776912 | MS.gene24683:CDS | 45.0% |
| !! | CTTAAACGACGTGTCGTTTG+TGG | + | chr7.3:9777169-9777188 | MS.gene24683:CDS | 45.0% |
| !! | TCACCGAAAGTGTCTTGCAT+GGG | + | chr7.3:9776894-9776913 | MS.gene24683:CDS | 45.0% |
| !!! | AGGATCCGTTTTGAGATCGT+CGG | - | chr7.3:9776532-9776551 | None:intergenic | 45.0% |
| !!! | TTTCGTGCTATTTTCCGCTC+CGG | + | chr7.3:9777035-9777054 | MS.gene24683:CDS | 45.0% |
| AATGATTCCGGCGGAGAATC+GGG | - | chr7.3:9776579-9776598 | None:intergenic | 50.0% | |
| ACGAGAAACAGCGTATCGGA+GGG | + | chr7.3:9777200-9777219 | MS.gene24683:CDS | 50.0% | |
| ATTCTCCGCCGGAATCATTC+TGG | + | chr7.3:9776580-9776599 | MS.gene24683:CDS | 50.0% | |
| CTGGAGAGCCAGAATGATTC+CGG | - | chr7.3:9776591-9776610 | None:intergenic | 50.0% | |
| GAATGATTCCGGCGGAGAAT+CGG | - | chr7.3:9776580-9776599 | None:intergenic | 50.0% | |
| GAGAAACGATTCTTCGGCGT+TGG | + | chr7.3:9777079-9777098 | MS.gene24683:CDS | 50.0% | |
| GGTGACGAGAAACAGCGTAT+CGG | + | chr7.3:9777196-9777215 | MS.gene24683:CDS | 50.0% | |
| GTTTATTCCTTCCACCGGAG+CGG | - | chr7.3:9777052-9777071 | None:intergenic | 50.0% | |
| TCTCAAAACGGATCCTCCAC+CGG | + | chr7.3:9776536-9776555 | MS.gene24683:CDS | 50.0% | |
| TTTCCGATCAGTTGAACCGC+CGG | - | chr7.3:9776490-9776509 | None:intergenic | 50.0% | |
| ! | TGATGATGAGAGGATTCCGG+TGG | - | chr7.3:9776555-9776574 | None:intergenic | 50.0% |
| !! | CGACGTGTCGTTTGTGGAAT+CGG | + | chr7.3:9777175-9777194 | MS.gene24683:CDS | 50.0% |
| !!! | CGCCGTTTTTTCTTTCACGG+CGG | - | chr7.3:9776973-9776992 | None:intergenic | 50.0% |
| ATCATCCCACCCAAACCAGG+CGG | - | chr7.3:9777230-9777249 | None:intergenic | 55.0% | |
| GACGAGAAACAGCGTATCGG+AGG | + | chr7.3:9777199-9777218 | MS.gene24683:CDS | 55.0% | |
| GAGAGCCAGAATGATTCCGG+CGG | - | chr7.3:9776588-9776607 | None:intergenic | 55.0% | |
| GCGTCGAAGGAAGGTTTCTG+AGG | - | chr7.3:9776786-9776805 | None:intergenic | 55.0% | |
| TCATCATCCCGATTCTCCGC+CGG | + | chr7.3:9776569-9776588 | MS.gene24683:CDS | 55.0% | |
| TGATGAGAGGATTCCGGTGG+AGG | - | chr7.3:9776552-9776571 | None:intergenic | 55.0% | |
| TGCGGTTCACGTCTGGAAGA+CGG | + | chr7.3:9777249-9777268 | MS.gene24683:CDS | 55.0% | |
| TGGAAGACGGTCTGAGTCGT+GGG | + | chr7.3:9777262-9777281 | MS.gene24683:CDS | 55.0% | |
| ! | CTATTTTCCGCTCCGGTGGA+AGG | + | chr7.3:9777042-9777061 | MS.gene24683:CDS | 55.0% |
| !! | ACACTGGTTTCAGCCTGTGC+CGG | + | chr7.3:9776468-9776487 | MS.gene24683:CDS | 55.0% |
| !! | CTGGTTTGGGTGGGATGATG+CGG | + | chr7.3:9777231-9777250 | MS.gene24683:CDS | 55.0% |
| AAGACGGTCTGAGTCGTGGG+TGG | + | chr7.3:9777265-9777284 | MS.gene24683:CDS | 60.0% | |
| ATCAGTTGAACCGCCGGCAC+AGG | - | chr7.3:9776484-9776503 | None:intergenic | 60.0% | |
| CGCATCATCCCACCCAAACC+AGG | - | chr7.3:9777233-9777252 | None:intergenic | 60.0% | |
| CTGGAAGACGGTCTGAGTCG+TGG | + | chr7.3:9777261-9777280 | MS.gene24683:CDS | 60.0% | |
| GAGGGTGAACCGCCTGGTTT+GGG | + | chr7.3:9777218-9777237 | MS.gene24683:CDS | 60.0% | |
| GGGATGATGCGGTTCACGTC+TGG | + | chr7.3:9777242-9777261 | MS.gene24683:CDS | 60.0% | |
| ! | CAAAGCGAAGCGCCTCCGTT+GGG | + | chr7.3:9776850-9776869 | MS.gene24683:CDS | 60.0% |
| ! | GTGCCGGCGGTTCAACTGAT+CGG | + | chr7.3:9776484-9776503 | MS.gene24683:CDS | 60.0% |
| ! | TTTCACGGCGGCTTTGGCTG+CGG | - | chr7.3:9776961-9776980 | None:intergenic | 60.0% |
| !! | ATCGTCGGCTTGTGTCTCGC+AGG | - | chr7.3:9776517-9776536 | None:intergenic | 60.0% |
| !! | GTGAACCGCCTGGTTTGGGT+GGG | + | chr7.3:9777222-9777241 | MS.gene24683:CDS | 60.0% |
| !!! | CGTGCTATTTTCCGCTCCGG+TGG | + | chr7.3:9777038-9777057 | MS.gene24683:CDS | 60.0% |
| CTGGTTTCAGCCTGTGCCGG+CGG | + | chr7.3:9776471-9776490 | MS.gene24683:CDS | 65.0% | |
| GGAGGGTGAACCGCCTGGTT+TGG | + | chr7.3:9777217-9777236 | MS.gene24683:CDS | 65.0% | |
| GTATCGGAGGGTGAACCGCC+TGG | + | chr7.3:9777212-9777231 | MS.gene24683:CDS | 65.0% | |
| ! | CCAAAGCGAAGCGCCTCCGT+TGG | + | chr7.3:9776849-9776868 | MS.gene24683:CDS | 65.0% |
| !! | CCAACGGAGGCGCTTCGCTT+TGG | - | chr7.3:9776852-9776871 | None:intergenic | 65.0% |
| !! | GGTGAACCGCCTGGTTTGGG+TGG | + | chr7.3:9777221-9777240 | MS.gene24683:CDS | 65.0% |
| ! | GGCTTTGGCTGCGGCGGTGA+CGG | - | chr7.3:9776952-9776971 | None:intergenic | 70.0% |
| ! | TTTGGCTGCGGCGGTGACGG+AGG | - | chr7.3:9776949-9776968 | None:intergenic | 70.0% |
| ! | CACGGCGGCTTTGGCTGCGG+CGG | - | chr7.3:9776958-9776977 | None:intergenic | 75.0% |
| !!! | GGCTGCGGCGGTGACGGAGG+AGG | - | chr7.3:9776946-9776965 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 9776453 | 9777316 | 9776453 | ID=MS.gene24683 |
| chr7.3 | mRNA | 9776453 | 9777316 | 9776453 | ID=MS.gene24683.t1;Parent=MS.gene24683 |
| chr7.3 | exon | 9776453 | 9777316 | 9776453 | ID=MS.gene24683.t1.exon1;Parent=MS.gene24683.t1 |
| chr7.3 | CDS | 9776453 | 9777316 | 9776453 | ID=cds.MS.gene24683.t1;Parent=MS.gene24683.t1 |
| Gene Sequence |
| Protein sequence |
