AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050309


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050309.t1 MTR_7g104725 97.578 289 7 0 1 289 1 289 0.0 570
MS.gene050309.t1 MTR_1g059890 43.871 310 119 14 1 287 3 280 6.73e-60 192
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050309.t1 AT3G52710 41.447 304 133 12 5 281 3 288 6.21e-49 164
MS.gene050309.t1 AT2G36220 40.702 285 128 12 2 277 4 256 6.10e-40 140

Find 75 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCGAATTGGAATTGGAATT+AGG 0.193499 7.2:-8201975 None:intergenic
ATTCTCCGCCGGAATCATTC+TGG 0.318910 7.2:+8201856 MS.gene050309:CDS
TCGAATTGGAATTGGAATTA+GGG 0.319492 7.2:-8201974 None:intergenic
AAGGAAGGTTTCTGAGGTTT+AGG 0.324775 7.2:-8202059 None:intergenic
GAGGGTGAACCGCCTGGTTT+GGG 0.327472 7.2:+8202500 MS.gene050309:CDS
GGAGGGTGAACCGCCTGGTT+TGG 0.332813 7.2:+8202499 MS.gene050309:CDS
CGACGTGTCGTTTGTGGAAT+CGG 0.337131 7.2:+8202457 MS.gene050309:CDS
CTTAAACGACGTGTCGTTTG+TGG 0.368356 7.2:+8202451 MS.gene050309:CDS
GGCTTTGGCTGCGGCGGTGA+CGG 0.374114 7.2:-8202231 None:intergenic
AATGATTCCGGCGGAGAATC+GGG 0.385576 7.2:-8201852 None:intergenic
AAATCGAAGACGTCGATGAT+TGG 0.397400 7.2:+8202032 MS.gene050309:CDS
CTGGAGAGCCAGAATGATTC+CGG 0.399676 7.2:-8201864 None:intergenic
CCAACGGAGGCGCTTCGCTT+TGG 0.403150 7.2:-8202131 None:intergenic
TGTTGAACTTGAATTTCCTT+TGG 0.404679 7.2:-8201943 None:intergenic
GGGATGATGCGGTTCACGTC+TGG 0.406943 7.2:+8202524 MS.gene050309:CDS
AGTCGAAGTCGAATTGGAAT+TGG 0.409214 7.2:-8201982 None:intergenic
TTTCCCATGCAAGACACTTT+CGG 0.424095 7.2:-8202179 None:intergenic
GAATGATTCCGGCGGAGAAT+CGG 0.431837 7.2:-8201853 None:intergenic
GTTGGAAGTCGAAGTCGAAT+TGG 0.435363 7.2:-8201988 None:intergenic
GGATGATGATGAGAGGATTC+CGG 0.444774 7.2:-8201831 None:intergenic
GGCTGCGGCGGTGACGGAGG+AGG 0.447604 7.2:-8202225 None:intergenic
CAAGACACTTTCGGTGATGA+AGG 0.466166 7.2:-8202170 None:intergenic
ATCAGTTGAACCGCCGGCAC+AGG 0.471514 7.2:-8201757 None:intergenic
GAGAAACGATTCTTCGGCGT+TGG 0.475237 7.2:+8202361 MS.gene050309:CDS
CACGGCGGCTTTGGCTGCGG+CGG 0.478127 7.2:-8202237 None:intergenic
ATCATGTTTATTCCTTCCAC+CGG 0.487247 7.2:-8202336 None:intergenic
GGTGAACCGCCTGGTTTGGG+TGG 0.490276 7.2:+8202503 MS.gene050309:CDS
TTTCACGGCGGCTTTGGCTG+CGG 0.490475 7.2:-8202240 None:intergenic
CGCATCATCCCACCCAAACC+AGG 0.492634 7.2:-8202512 None:intergenic
GGAAACAACTTAATGTTACT+CGG 0.496570 7.2:-8202110 None:intergenic
CAAAGCGAAGCGCCTCCGTT+GGG 0.503793 7.2:+8202132 MS.gene050309:CDS
CTGGAAGACGGTCTGAGTCG+TGG 0.507701 7.2:+8202543 MS.gene050309:CDS
CCAAAGCGAAGCGCCTCCGT+TGG 0.522856 7.2:+8202131 MS.gene050309:CDS
GTATCGGAGGGTGAACCGCC+TGG 0.524644 7.2:+8202494 MS.gene050309:CDS
GTGAACCGCCTGGTTTGGGT+GGG 0.526851 7.2:+8202504 MS.gene050309:CDS
ATTTGCGAATCGCTGAGAGT+TGG 0.530028 7.2:-8202006 None:intergenic
TGATCGGAGAAACGATTCTT+CGG 0.530088 7.2:+8202355 MS.gene050309:CDS
TGGAAGACGGTCTGAGTCGT+GGG 0.533984 7.2:+8202544 MS.gene050309:CDS
AGATGAAGAAAACGAGTGGT+TGG 0.534269 7.2:+8201887 MS.gene050309:CDS
ACACTGGTTTCAGCCTGTGC+CGG 0.536079 7.2:+8201744 MS.gene050309:CDS
TGATGAGAGGATTCCGGTGG+AGG 0.536818 7.2:-8201825 None:intergenic
TTTGGCTGCGGCGGTGACGG+AGG 0.537701 7.2:-8202228 None:intergenic
AGATTCTTAGCGTCGAAGGA+AGG 0.541094 7.2:-8202074 None:intergenic
TCTCAAAACGGATCCTCCAC+CGG 0.544913 7.2:+8201812 MS.gene050309:CDS
ATCGTCGGCTTGTGTCTCGC+AGG 0.552782 7.2:-8201790 None:intergenic
GTGCCGGCGGTTCAACTGAT+CGG 0.557681 7.2:+8201760 MS.gene050309:CDS
CTGGTTTGGGTGGGATGATG+CGG 0.557941 7.2:+8202513 MS.gene050309:CDS
CTGGTTTCAGCCTGTGCCGG+CGG 0.561067 7.2:+8201747 MS.gene050309:CDS
TTCAAAAGCGCGTGATAGCA+CGG 0.567098 7.2:+8202424 MS.gene050309:CDS
TCATCATCCCGATTCTCCGC+CGG 0.570450 7.2:+8201845 MS.gene050309:CDS
TTTCCGATCAGTTGAACCGC+CGG 0.577458 7.2:-8201763 None:intergenic
TCTCAAGGATATGCTACAAG+TGG 0.579670 7.2:+8201719 None:intergenic
ATCACCGAAAGTGTCTTGCA+TGG 0.582694 7.2:+8202175 MS.gene050309:CDS
ACAAGCCGACGATCTCAAAA+CGG 0.592406 7.2:+8201800 MS.gene050309:CDS
TCACCGAAAGTGTCTTGCAT+GGG 0.596284 7.2:+8202176 MS.gene050309:CDS
ACGAAGATTCTTAGCGTCGA+AGG 0.596920 7.2:-8202078 None:intergenic
GACGAGAAACAGCGTATCGG+AGG 0.603677 7.2:+8202481 MS.gene050309:CDS
GCGTCGAAGGAAGGTTTCTG+AGG 0.608308 7.2:-8202065 None:intergenic
GTGGAAGGAATAAACATGAT+CGG 0.609068 7.2:+8202339 MS.gene050309:CDS
TGCGGTTCACGTCTGGAAGA+CGG 0.620278 7.2:+8202531 MS.gene050309:CDS
GAACGCAAAGTATCAACCAA+AGG 0.624704 7.2:+8201927 MS.gene050309:CDS
GTTTATTCCTTCCACCGGAG+CGG 0.629764 7.2:-8202331 None:intergenic
TATGCTACAAGTGGAAACAC+TGG 0.636979 7.2:+8201728 None:intergenic
ACGAATACTATCTTGACACG+TGG 0.641215 7.2:-8202583 None:intergenic
AACAAACGATTTCCCAACGG+AGG 0.643622 7.2:-8202144 None:intergenic
AAGACGGTCTGAGTCGTGGG+TGG 0.657734 7.2:+8202547 MS.gene050309:CDS
TGATGATGAGAGGATTCCGG+TGG 0.659739 7.2:-8201828 None:intergenic
GGTGACGAGAAACAGCGTAT+CGG 0.661202 7.2:+8202478 MS.gene050309:CDS
AGAATCGGGATGATGATGAG+AGG 0.662087 7.2:-8201838 None:intergenic
TTCAACAAACGATTTCCCAA+CGG 0.663139 7.2:-8202147 None:intergenic
ACGAGAAACAGCGTATCGGA+GGG 0.675020 7.2:+8202482 MS.gene050309:CDS
CCAGAGATGAAGAAAACGAG+TGG 0.676074 7.2:+8201883 MS.gene050309:CDS
GAGAGCCAGAATGATTCCGG+CGG 0.678279 7.2:-8201861 None:intergenic
ATCATCCCACCCAAACCAGG+CGG 0.701016 7.2:-8202509 None:intergenic
GGAAAAGTCAGATCTAAACG+CGG 0.786700 7.2:+8202197 MS.gene050309:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GGAAACAACTTAATGTTACT+CGG - chr7.2:8202113-8202132 None:intergenic 30.0%
TCGAATTGGAATTGGAATTA+GGG - chr7.2:8201977-8201996 None:intergenic 30.0%
TGTTGAACTTGAATTTCCTT+TGG - chr7.2:8201946-8201965 None:intergenic 30.0%
!!! GTGTTTTGATTGTTTTCTCT+CGG - chr7.2:8202282-8202301 None:intergenic 30.0%
ATCATGTTTATTCCTTCCAC+CGG - chr7.2:8202339-8202358 None:intergenic 35.0%
GTCGAATTGGAATTGGAATT+AGG - chr7.2:8201978-8201997 None:intergenic 35.0%
GTGGAAGGAATAAACATGAT+CGG + chr7.2:8202339-8202358 MS.gene050309:CDS 35.0%
TTCAACAAACGATTTCCCAA+CGG - chr7.2:8202150-8202169 None:intergenic 35.0%
AAATCGAAGACGTCGATGAT+TGG + chr7.2:8202032-8202051 MS.gene050309:CDS 40.0%
AGATGAAGAAAACGAGTGGT+TGG + chr7.2:8201887-8201906 MS.gene050309:CDS 40.0%
AGTCGAAGTCGAATTGGAAT+TGG - chr7.2:8201985-8202004 None:intergenic 40.0%
GGAAAAGTCAGATCTAAACG+CGG + chr7.2:8202197-8202216 MS.gene050309:CDS 40.0%
TGATCGGAGAAACGATTCTT+CGG + chr7.2:8202355-8202374 MS.gene050309:CDS 40.0%
TTTCCCATGCAAGACACTTT+CGG - chr7.2:8202182-8202201 None:intergenic 40.0%
! GAACGCAAAGTATCAACCAA+AGG + chr7.2:8201927-8201946 MS.gene050309:CDS 40.0%
!! AAGGAAGGTTTCTGAGGTTT+AGG - chr7.2:8202062-8202081 None:intergenic 40.0%
!!! TCTCGCCGTTTTTTCTTTCA+CGG - chr7.2:8202258-8202277 None:intergenic 40.0%
AACAAACGATTTCCCAACGG+AGG - chr7.2:8202147-8202166 None:intergenic 45.0%
ACAAGCCGACGATCTCAAAA+CGG + chr7.2:8201800-8201819 MS.gene050309:CDS 45.0%
AGCCGCCGTGAAAGAAAAAA+CGG + chr7.2:8202250-8202269 MS.gene050309:CDS 45.0%
ATTTGCGAATCGCTGAGAGT+TGG - chr7.2:8202009-8202028 None:intergenic 45.0%
CAAGACACTTTCGGTGATGA+AGG - chr7.2:8202173-8202192 None:intergenic 45.0%
CCAGAGATGAAGAAAACGAG+TGG + chr7.2:8201883-8201902 MS.gene050309:CDS 45.0%
GTTGGAAGTCGAAGTCGAAT+TGG - chr7.2:8201991-8202010 None:intergenic 45.0%
! ACGAAGATTCTTAGCGTCGA+AGG - chr7.2:8202081-8202100 None:intergenic 45.0%
! AGAATCGGGATGATGATGAG+AGG - chr7.2:8201841-8201860 None:intergenic 45.0%
! AGATTCTTAGCGTCGAAGGA+AGG - chr7.2:8202077-8202096 None:intergenic 45.0%
! CCACTCGTTTTCTTCATCTC+TGG - chr7.2:8201886-8201905 None:intergenic 45.0%
! GGATGATGATGAGAGGATTC+CGG - chr7.2:8201834-8201853 None:intergenic 45.0%
! TTCAAAAGCGCGTGATAGCA+CGG + chr7.2:8202424-8202443 MS.gene050309:CDS 45.0%
! TTTTTCTTTCACGGCGGCTT+TGG - chr7.2:8202249-8202268 None:intergenic 45.0%
!! ATCACCGAAAGTGTCTTGCA+TGG + chr7.2:8202175-8202194 MS.gene050309:CDS 45.0%
!! CTTAAACGACGTGTCGTTTG+TGG + chr7.2:8202451-8202470 MS.gene050309:CDS 45.0%
!! TCACCGAAAGTGTCTTGCAT+GGG + chr7.2:8202176-8202195 MS.gene050309:CDS 45.0%
!!! AGGATCCGTTTTGAGATCGT+CGG - chr7.2:8201808-8201827 None:intergenic 45.0%
!!! TTTCGTGCTATTTTCCGCTC+CGG + chr7.2:8202317-8202336 MS.gene050309:CDS 45.0%
AATGATTCCGGCGGAGAATC+GGG - chr7.2:8201855-8201874 None:intergenic 50.0%
ACGAGAAACAGCGTATCGGA+GGG + chr7.2:8202482-8202501 MS.gene050309:CDS 50.0%
ATTCTCCGCCGGAATCATTC+TGG + chr7.2:8201856-8201875 MS.gene050309:CDS 50.0%
CTGGAGAGCCAGAATGATTC+CGG - chr7.2:8201867-8201886 None:intergenic 50.0%
GAATGATTCCGGCGGAGAAT+CGG - chr7.2:8201856-8201875 None:intergenic 50.0%
GAGAAACGATTCTTCGGCGT+TGG + chr7.2:8202361-8202380 MS.gene050309:CDS 50.0%
GGTGACGAGAAACAGCGTAT+CGG + chr7.2:8202478-8202497 MS.gene050309:CDS 50.0%
GTTTATTCCTTCCACCGGAG+CGG - chr7.2:8202334-8202353 None:intergenic 50.0%
TCTCAAAACGGATCCTCCAC+CGG + chr7.2:8201812-8201831 MS.gene050309:CDS 50.0%
TTTCCGATCAGTTGAACCGC+CGG - chr7.2:8201766-8201785 None:intergenic 50.0%
! TGATGATGAGAGGATTCCGG+TGG - chr7.2:8201831-8201850 None:intergenic 50.0%
!! CGACGTGTCGTTTGTGGAAT+CGG + chr7.2:8202457-8202476 MS.gene050309:CDS 50.0%
!!! CGCCGTTTTTTCTTTCACGG+CGG - chr7.2:8202255-8202274 None:intergenic 50.0%
ATCATCCCACCCAAACCAGG+CGG - chr7.2:8202512-8202531 None:intergenic 55.0%
GACGAGAAACAGCGTATCGG+AGG + chr7.2:8202481-8202500 MS.gene050309:CDS 55.0%
GAGAGCCAGAATGATTCCGG+CGG - chr7.2:8201864-8201883 None:intergenic 55.0%
GCGTCGAAGGAAGGTTTCTG+AGG - chr7.2:8202068-8202087 None:intergenic 55.0%
TCATCATCCCGATTCTCCGC+CGG + chr7.2:8201845-8201864 MS.gene050309:CDS 55.0%
TGATGAGAGGATTCCGGTGG+AGG - chr7.2:8201828-8201847 None:intergenic 55.0%
TGCGGTTCACGTCTGGAAGA+CGG + chr7.2:8202531-8202550 MS.gene050309:CDS 55.0%
TGGAAGACGGTCTGAGTCGT+GGG + chr7.2:8202544-8202563 MS.gene050309:CDS 55.0%
! CTATTTTCCGCTCCGGTGGA+AGG + chr7.2:8202324-8202343 MS.gene050309:CDS 55.0%
!! ACACTGGTTTCAGCCTGTGC+CGG + chr7.2:8201744-8201763 MS.gene050309:CDS 55.0%
!! CTGGTTTGGGTGGGATGATG+CGG + chr7.2:8202513-8202532 MS.gene050309:CDS 55.0%
AAGACGGTCTGAGTCGTGGG+TGG + chr7.2:8202547-8202566 MS.gene050309:CDS 60.0%
ATCAGTTGAACCGCCGGCAC+AGG - chr7.2:8201760-8201779 None:intergenic 60.0%
CGCATCATCCCACCCAAACC+AGG - chr7.2:8202515-8202534 None:intergenic 60.0%
CTGGAAGACGGTCTGAGTCG+TGG + chr7.2:8202543-8202562 MS.gene050309:CDS 60.0%
GAGGGTGAACCGCCTGGTTT+GGG + chr7.2:8202500-8202519 MS.gene050309:CDS 60.0%
GGGATGATGCGGTTCACGTC+TGG + chr7.2:8202524-8202543 MS.gene050309:CDS 60.0%
! CAAAGCGAAGCGCCTCCGTT+GGG + chr7.2:8202132-8202151 MS.gene050309:CDS 60.0%
! GTGCCGGCGGTTCAACTGAT+CGG + chr7.2:8201760-8201779 MS.gene050309:CDS 60.0%
! TTTCACGGCGGCTTTGGCTG+CGG - chr7.2:8202243-8202262 None:intergenic 60.0%
!! ATCGTCGGCTTGTGTCTCGC+AGG - chr7.2:8201793-8201812 None:intergenic 60.0%
!! GTGAACCGCCTGGTTTGGGT+GGG + chr7.2:8202504-8202523 MS.gene050309:CDS 60.0%
!!! CGTGCTATTTTCCGCTCCGG+TGG + chr7.2:8202320-8202339 MS.gene050309:CDS 60.0%
CTGGTTTCAGCCTGTGCCGG+CGG + chr7.2:8201747-8201766 MS.gene050309:CDS 65.0%
GGAGGGTGAACCGCCTGGTT+TGG + chr7.2:8202499-8202518 MS.gene050309:CDS 65.0%
GTATCGGAGGGTGAACCGCC+TGG + chr7.2:8202494-8202513 MS.gene050309:CDS 65.0%
! CCAAAGCGAAGCGCCTCCGT+TGG + chr7.2:8202131-8202150 MS.gene050309:CDS 65.0%
!! CCAACGGAGGCGCTTCGCTT+TGG - chr7.2:8202134-8202153 None:intergenic 65.0%
!! GGTGAACCGCCTGGTTTGGG+TGG + chr7.2:8202503-8202522 MS.gene050309:CDS 65.0%
! GGCTTTGGCTGCGGCGGTGA+CGG - chr7.2:8202234-8202253 None:intergenic 70.0%
! TTTGGCTGCGGCGGTGACGG+AGG - chr7.2:8202231-8202250 None:intergenic 70.0%
! CACGGCGGCTTTGGCTGCGG+CGG - chr7.2:8202240-8202259 None:intergenic 75.0%
!!! GGCTGCGGCGGTGACGGAGG+AGG - chr7.2:8202228-8202247 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 8201729 8202598 8201729 ID=MS.gene050309
chr7.2 mRNA 8201729 8202598 8201729 ID=MS.gene050309.t1;Parent=MS.gene050309
chr7.2 exon 8201729 8202598 8201729 ID=MS.gene050309.t1.exon1;Parent=MS.gene050309.t1
chr7.2 CDS 8201729 8202598 8201729 ID=cds.MS.gene050309.t1;Parent=MS.gene050309.t1
Gene Sequence

>MS.gene050309

ATGCTACAAGTGGAAACACTGGTTTCAGCCTGTGCCGGCGGTTCAACTGATCGGAAAATCGCCTGCGAGACACAAGCCGACGATCTCAAAACGGATCCTCCACCGGAATCCTCTCATCATCATCCCGATTCTCCGCCGGAATCATTCTGGCTCTCCAGAGATGAAGAAAACGAGTGGTTGGATCGCAATTTCATCTACGAACGCAAAGTATCAACCAAAGGAAATTCAAGTTCAACAAACTTAAACCCTAATTCCAATTCCAATTCGACTTCGACTTCCAACTCTCAGCGATTCGCAAATCTCAAATCGAAGACGTCGATGATTGGTTTGCCTAAACCTCAGAAACCTTCCTTCGACGCTAAGAATCTTCGTAATCACAAGCCGAGTAACATTAAGTTGTTTCCAAAGCGAAGCGCCTCCGTTGGGAAATCGTTTGTTGAACCTTCATCACCGAAAGTGTCTTGCATGGGAAAAGTCAGATCTAAACGCGGTAAAACCTCCTCCGTCACCGCCGCAGCCAAAGCCGCCGTGAAAGAAAAAACGGCGAGAACCGAGAGAAAACAATCAAAACACAGTTTCTTTAAAAGTTTTCGTGCTATTTTCCGCTCCGGTGGAAGGAATAAACATGATCGGAGAAACGATTCTTCGGCGTTGGATTCAAGTTCAATCATGAAGAACACGAACAGCGTGAATGATTCAAAAGCGCGTGATAGCACGGCGAGCTTAAACGACGTGTCGTTTGTGGAATCGGTGACGAGAAACAGCGTATCGGAGGGTGAACCGCCTGGTTTGGGTGGGATGATGCGGTTCACGTCTGGAAGACGGTCTGAGTCGTGGGTGGATGATTCTGAAATCCACGTGTCAAGATAG

Protein sequence

>MS.gene050309.t1

MLQVETLVSACAGGSTDRKIACETQADDLKTDPPPESSHHHPDSPPESFWLSRDEENEWLDRNFIYERKVSTKGNSSSTNLNPNSNSNSTSTSNSQRFANLKSKTSMIGLPKPQKPSFDAKNLRNHKPSNIKLFPKRSASVGKSFVEPSSPKVSCMGKVRSKRGKTSSVTAAAKAAVKEKTARTERKQSKHSFFKSFRAIFRSGGRNKHDRRNDSSALDSSSIMKNTNSVNDSKARDSTASLNDVSFVESVTRNSVSEGEPPGLGGMMRFTSGRRSESWVDDSEIHVSR