Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24829.t1 | XP_013458419.1 | 96.1 | 970 | 38 | 0 | 35 | 1004 | 32 | 1001 | 0.00E+00 | 1243.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24829.t1 | Q6NXD8 | 31.1 | 119 | 76 | 2 | 808 | 920 | 304 | 422 | 3.4e-08 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24829.t1 | A0A072V2V8 | 96.1 | 970 | 38 | 0 | 35 | 1004 | 32 | 1001 | 0.0e+00 | 1243.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051817 | MS.gene24829 | 0.985376 | 2.24E-163 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24829.t1 | MTR_4g125480 | 94.129 | 1005 | 54 | 3 | 1 | 1004 | 1 | 1001 | 0.0 | 1927 |
| MS.gene24829.t1 | MTR_4g125480 | 94.004 | 984 | 54 | 3 | 1 | 983 | 1 | 980 | 0.0 | 1883 |
| MS.gene24829.t1 | MTR_4g125480 | 92.073 | 984 | 53 | 4 | 1 | 983 | 1 | 960 | 0.0 | 1833 |
| MS.gene24829.t1 | MTR_2g041470 | 74.707 | 427 | 106 | 2 | 579 | 1004 | 357 | 782 | 0.0 | 652 |
| MS.gene24829.t1 | MTR_2g041470 | 67.742 | 403 | 118 | 4 | 7 | 401 | 25 | 423 | 1.39e-168 | 514 |
| MS.gene24829.t1 | MTR_2g041470 | 67.910 | 402 | 117 | 4 | 7 | 400 | 25 | 422 | 5.27e-169 | 514 |
| MS.gene24829.t1 | MTR_2g041470 | 70.769 | 325 | 93 | 2 | 579 | 902 | 357 | 680 | 1.15e-150 | 466 |
| MS.gene24829.t1 | MTR_7g099030 | 48.632 | 329 | 162 | 3 | 693 | 1021 | 501 | 822 | 5.87e-87 | 298 |
| MS.gene24829.t1 | MTR_7g099030 | 42.697 | 267 | 146 | 3 | 32 | 292 | 73 | 338 | 1.68e-58 | 217 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24829.t1 | AT5G35560 | 63.660 | 388 | 124 | 4 | 619 | 1004 | 345 | 717 | 1.33e-162 | 497 |
| MS.gene24829.t1 | AT5G35560 | 62.007 | 279 | 95 | 4 | 17 | 293 | 42 | 311 | 9.43e-108 | 352 |
| MS.gene24829.t1 | AT5G35560 | 63.660 | 388 | 124 | 4 | 619 | 1004 | 371 | 743 | 2.36e-162 | 497 |
| MS.gene24829.t1 | AT5G35560 | 62.007 | 279 | 95 | 4 | 17 | 293 | 42 | 311 | 1.36e-107 | 353 |
| MS.gene24829.t1 | AT2G20320 | 50.333 | 300 | 142 | 3 | 707 | 1004 | 711 | 1005 | 4.28e-89 | 308 |
| MS.gene24829.t1 | AT2G20320 | 42.537 | 268 | 144 | 4 | 32 | 292 | 284 | 548 | 1.82e-61 | 228 |
| MS.gene24829.t1 | AT2G20320 | 50.333 | 300 | 142 | 3 | 707 | 1004 | 711 | 1005 | 4.28e-89 | 308 |
| MS.gene24829.t1 | AT2G20320 | 42.537 | 268 | 144 | 4 | 32 | 292 | 284 | 548 | 1.82e-61 | 228 |
Find 237 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGACAATTCAACACTTTA+TGG | 0.115504 | 8.4:-9421785 | MS.gene24829:CDS |
| CCGATCTTCTAATTCTTTAT+TGG | 0.135191 | 8.4:+9419673 | None:intergenic |
| ATGACAGATCCATTTGTTTA+AGG | 0.146395 | 8.4:+9421949 | None:intergenic |
| TTGAGAGCAGTGAGGATTTC+AGG | 0.206301 | 8.4:+9423646 | None:intergenic |
| GTCATTTGTATTCAGGTTAC+AGG | 0.228632 | 8.4:-9421931 | MS.gene24829:intron |
| TGGATCTGTCATTTGTATTC+AGG | 0.234584 | 8.4:-9421938 | MS.gene24829:CDS |
| ATAAGAATACTCACTATATA+AGG | 0.235466 | 8.4:+9416613 | None:intergenic |
| TTCGGTGTTGAGGTCTTATC+TGG | 0.238265 | 8.4:-9415388 | MS.gene24829:CDS |
| ACCGGATTGGTTGTCCTTCC+AGG | 0.252367 | 8.4:-9418866 | MS.gene24829:CDS |
| AGTTGTTGCCTGCTCTAATT+TGG | 0.255522 | 8.4:-9417584 | MS.gene24829:intron |
| CCACTATCTTCAGAAATTCT+AGG | 0.263141 | 8.4:+9419866 | None:intergenic |
| TCACTATCTTCAGAAATTAT+AGG | 0.264701 | 8.4:+9420451 | None:intergenic |
| AACTGTAGGGCCAGGTTCTT+TGG | 0.269822 | 8.4:+9420321 | None:intergenic |
| AGTTCCTGAACATCACAATT+AGG | 0.284358 | 8.4:+9423340 | None:intergenic |
| ATCACTTACATTTCATCCTT+TGG | 0.288504 | 8.4:-9418838 | MS.gene24829:CDS |
| GCAGCTGCCCGTCAACTATT+TGG | 0.292722 | 8.4:+9419975 | None:intergenic |
| TGTTGGTTTCACAGGGTTAT+AGG | 0.294641 | 8.4:-9423417 | MS.gene24829:intron |
| CAGTACCTGAGGTTCAATAT+TGG | 0.300865 | 8.4:+9423231 | None:intergenic |
| TGGAGAACTTCAGTCTTATA+AGG | 0.303705 | 8.4:-9420401 | MS.gene24829:CDS |
| TGCACTTTGGTGTATTAAAT+AGG | 0.308629 | 8.4:-9421610 | MS.gene24829:intron |
| CTTCTTTCTTTCTGTTTCCC+TGG | 0.313717 | 8.4:-9422963 | MS.gene24829:CDS |
| TCTAGGGAAAGATTCTTCTA+TGG | 0.316438 | 8.4:+9419883 | None:intergenic |
| TGTAGTGCCAGATTCTTCTT+TGG | 0.319307 | 8.4:+9420177 | None:intergenic |
| AAATTATATGCGAGTATTAC+CGG | 0.320510 | 8.4:-9418884 | MS.gene24829:CDS |
| GATGTTACTGCATTCTTTCA+AGG | 0.321969 | 8.4:-9421649 | MS.gene24829:CDS |
| AGATTCTTCTTTGGCTGCTT+CGG | 0.325461 | 8.4:+9420186 | None:intergenic |
| CGGGCAGCTGCATCCTTATA+AGG | 0.328318 | 8.4:-9419963 | MS.gene24829:CDS |
| TGTACGGCAAGGTTCTTCTT+TGG | 0.336203 | 8.4:+9420030 | None:intergenic |
| ACTTTATGGGTGTTGTGTTT+TGG | 0.340075 | 8.4:-9421771 | MS.gene24829:CDS |
| AGTACCTGAGGTTCAATATT+GGG | 0.340930 | 8.4:+9423232 | None:intergenic |
| CCTTATCACGCAGCTCTTGT+AGG | 0.343628 | 8.4:-9415721 | MS.gene24829:CDS |
| TAATCTATTCTCTCCTTATA+AGG | 0.343634 | 8.4:+9419950 | None:intergenic |
| CTGACAATTCAACACTTTAT+GGG | 0.346432 | 8.4:-9421784 | MS.gene24829:CDS |
| GCAGCTCTCCATCAACTATT+AGG | 0.347447 | 8.4:+9420560 | None:intergenic |
| TGGAGAGCTGCGGTCATATA+AGG | 0.348818 | 8.4:-9420548 | MS.gene24829:CDS |
| AGCTGCAAAGGGGTTTCTTT+CGG | 0.351895 | 8.4:-9415406 | MS.gene24829:CDS |
| TCACCCTAATTGTGATGTTC+AGG | 0.360223 | 8.4:-9423344 | MS.gene24829:CDS |
| CCTGAGAACCTAATAGTTGA+TGG | 0.361614 | 8.4:-9420568 | MS.gene24829:CDS |
| CTTGGCAGTTGTGGTATTGT+TGG | 0.362762 | 8.4:+9415772 | None:intergenic |
| TGAAGATAGTGGTGGTCATC+TGG | 0.362819 | 8.4:-9419855 | MS.gene24829:CDS |
| TTTCTGTTTCCCTGGAGGCT+TGG | 0.364156 | 8.4:-9422955 | MS.gene24829:CDS |
| TGGAGAGATACAGTCATATA+AGG | 0.366811 | 8.4:-9420110 | MS.gene24829:CDS |
| TTATCAGTTTCATGCTTAGT+AGG | 0.367671 | 8.4:+9419914 | None:intergenic |
| CCATCAACTATTAGGTTCTC+AGG | 0.368919 | 8.4:+9420568 | None:intergenic |
| CACTATCTTCAGAAATTATA+GGG | 0.380078 | 8.4:+9420452 | None:intergenic |
| AGAAGATCCTTGCATTTAAG+AGG | 0.384174 | 8.4:+9422981 | None:intergenic |
| ACGATTCAGAGCTTTCATAC+TGG | 0.387340 | 8.4:+9419612 | None:intergenic |
| CACTGCTCTCAAGCGTCAAT+GGG | 0.396144 | 8.4:-9423635 | MS.gene24829:CDS |
| CCTACAAGAGCTGCGTGATA+AGG | 0.400743 | 8.4:+9415721 | None:intergenic |
| CCATCTACCATTAGGTTCTC+CGG | 0.403077 | 8.4:+9420130 | None:intergenic |
| CCGTCAACTATTTGGTTCTC+CGG | 0.403550 | 8.4:+9419983 | None:intergenic |
| CTGGAGAATGCAATGGTTGA+TGG | 0.404044 | 8.4:-9419836 | MS.gene24829:CDS |
| GAAGTTCTCCAACAACCATT+AGG | 0.405095 | 8.4:+9420413 | None:intergenic |
| CTGTTTCCCTGGAGGCTTGG+AGG | 0.408696 | 8.4:-9422952 | MS.gene24829:intron |
| TCACTGCTCTCAAGCGTCAA+TGG | 0.411870 | 8.4:-9423636 | MS.gene24829:CDS |
| TGCATAGGCAATTTGTTGAT+AGG | 0.412840 | 8.4:-9423318 | MS.gene24829:CDS |
| TATAGGGCCAGATTCTTCAT+TGG | 0.414422 | 8.4:+9420468 | None:intergenic |
| CTACTGAGCATGAAACTGAT+AGG | 0.419168 | 8.4:-9420060 | MS.gene24829:CDS |
| TTCGAAAGCATGGTGGTTGT+TGG | 0.419586 | 8.4:-9423370 | MS.gene24829:CDS |
| ACTTAAATGATATTCTTGAA+TGG | 0.419604 | 8.4:-9419023 | MS.gene24829:CDS |
| TTAAGTCATTTAAATTCTCT+TGG | 0.421502 | 8.4:+9419040 | None:intergenic |
| CAGTCTTATAAGGACAGAAT+AGG | 0.422776 | 8.4:-9420391 | MS.gene24829:CDS |
| GATATTAGCTCTTTCTGTCT+TGG | 0.423417 | 8.4:+9415754 | None:intergenic |
| TAACATTGGTAAACTTTGAC+TGG | 0.428914 | 8.4:+9416223 | None:intergenic |
| GTATCTCTCCATCTACCATT+AGG | 0.431400 | 8.4:+9420122 | None:intergenic |
| CTACAAGAGCTGCGTGATAA+GGG | 0.433410 | 8.4:+9415722 | None:intergenic |
| TCTGAAGACAATGATCAGTA+TGG | 0.434045 | 8.4:-9419710 | MS.gene24829:CDS |
| CCTAGAATTTCTGAAGATAG+TGG | 0.434304 | 8.4:-9419866 | MS.gene24829:CDS |
| GTTCCTGAACATCACAATTA+GGG | 0.435119 | 8.4:+9423341 | None:intergenic |
| TTTGGAGCACCTGCATCCCT+TGG | 0.438368 | 8.4:-9418820 | MS.gene24829:CDS |
| ATCTTCTAATTCTTTATTGG+TGG | 0.438856 | 8.4:+9419676 | None:intergenic |
| CCAACAACCATTAGGTTCTC+CGG | 0.441190 | 8.4:+9420421 | None:intergenic |
| TCAAGTTACTAAATTGTTCA+AGG | 0.442467 | 8.4:-9423707 | MS.gene24829:CDS |
| CCGGAGAACCTAATGGTTGT+TGG | 0.442593 | 8.4:-9420421 | MS.gene24829:CDS |
| TGGACTACTGCTTGCTTATG+TGG | 0.443811 | 8.4:-9418521 | MS.gene24829:CDS |
| CTTAAATGATATTCTTGAAT+GGG | 0.444172 | 8.4:-9419022 | MS.gene24829:CDS |
| GAAGCAGCCAAAGAAGAATC+TGG | 0.445818 | 8.4:-9420184 | MS.gene24829:CDS |
| GAAAAGTTGCAGTACCGGTC+TGG | 0.446659 | 8.4:-9418742 | MS.gene24829:CDS |
| GCAGGTCTCCATCAACCATT+AGG | 0.449721 | 8.4:+9420269 | None:intergenic |
| CCGGATTGGTTGTCCTTCCA+GGG | 0.449866 | 8.4:-9418865 | MS.gene24829:CDS |
| CGATTCAGAGCTTTCATACT+GGG | 0.451992 | 8.4:+9419613 | None:intergenic |
| CTACAGATTGGAGCTGCAAA+GGG | 0.455645 | 8.4:-9415417 | MS.gene24829:intron |
| GCAGGAACACCTTAAACAAA+TGG | 0.458315 | 8.4:-9421958 | MS.gene24829:intron |
| CCGGAGAACCTAATGGTAGA+TGG | 0.459592 | 8.4:-9420130 | MS.gene24829:CDS |
| CTACGACGAATATTAGCAAT+AGG | 0.464254 | 8.4:-9412727 | MS.gene24829:CDS |
| TCAACGGCCAATGAAGAATC+TGG | 0.464288 | 8.4:-9420475 | MS.gene24829:CDS |
| CAGGTGAACCTAATGGTTGA+TGG | 0.464377 | 8.4:-9420277 | MS.gene24829:CDS |
| AGCAGGCTTTGCCATCGATA+AGG | 0.465137 | 8.4:+9416791 | None:intergenic |
| TCGACATCTGAAAACATTGT+TGG | 0.466403 | 8.4:-9420601 | MS.gene24829:CDS |
| CACCTAACCTCCAAGCCTCC+AGG | 0.466727 | 8.4:+9422945 | None:intergenic |
| TTGGAATATCATAGACCATC+CGG | 0.470147 | 8.4:-9418801 | MS.gene24829:CDS |
| ACAACAGCAAAAGAAGAATC+TGG | 0.471224 | 8.4:-9419743 | MS.gene24829:CDS |
| GATACTGCAACCTTTGAAGT+TGG | 0.478141 | 8.4:+9423728 | None:intergenic |
| TGTACAATCAAATGACGATA+AGG | 0.478142 | 8.4:-9415325 | MS.gene24829:intron |
| ATTCTTAGCAGGAATTCTGC+TGG | 0.483107 | 8.4:-9417617 | MS.gene24829:CDS |
| TCATTGTCTTCAGAAATTGT+AGG | 0.483670 | 8.4:+9419719 | None:intergenic |
| TAAGAATACTCACTATATAA+GGG | 0.485316 | 8.4:+9416614 | None:intergenic |
| GGCTTTGCCATCGATAAGGC+TGG | 0.485441 | 8.4:+9416795 | None:intergenic |
| AGGCAACTTCGTTATCTGTA+TGG | 0.486973 | 8.4:-9418541 | MS.gene24829:CDS |
| AATTTCATTCAACTCACTCA+TGG | 0.487854 | 8.4:+9422302 | None:intergenic |
| GAACTAGTGCAAAAGCCCTC+TGG | 0.488144 | 8.4:-9421743 | MS.gene24829:CDS |
| AAACTTTGACTGGACTTCAC+TGG | 0.488522 | 8.4:+9416233 | None:intergenic |
| GATTTGAAAAGTTGCAGTAC+CGG | 0.488753 | 8.4:-9418747 | MS.gene24829:CDS |
| TGGAGACCTGCAGTCTCATA+AGG | 0.491106 | 8.4:-9420257 | MS.gene24829:CDS |
| TCAAATTTAGAATTTGTTGA+AGG | 0.492252 | 8.4:-9420739 | MS.gene24829:CDS |
| TTTCACAGGGTTATAGGTCG+TGG | 0.492407 | 8.4:-9423411 | MS.gene24829:intron |
| GGGACAGTCCTGTGTCGTAC+TGG | 0.492408 | 8.4:-9418780 | MS.gene24829:CDS |
| CTCACGGCTGTTCGAAAGCA+TGG | 0.494790 | 8.4:-9423380 | MS.gene24829:CDS |
| CATTGTCTTCAGAAATTGTA+GGG | 0.497709 | 8.4:+9419720 | None:intergenic |
| CCCTGGAAGGACAACCAATC+CGG | 0.498435 | 8.4:+9418865 | None:intergenic |
| CGTCAACTATTTGGTTCTCC+GGG | 0.501380 | 8.4:+9419984 | None:intergenic |
| TTATTGTGGTTGATGCCAAC+AGG | 0.502457 | 8.4:-9416203 | MS.gene24829:CDS |
| TATGATATTCCAAGGGATGC+AGG | 0.505823 | 8.4:+9418811 | None:intergenic |
| TGAAAGGCCGATCGCGATGC+GGG | 0.506734 | 8.4:+9414161 | None:intergenic |
| TGTCCTATCAATTTCATGCT+CGG | 0.509586 | 8.4:+9419763 | None:intergenic |
| CAGGTGTTAACATTCTTAGC+AGG | 0.509682 | 8.4:-9417628 | MS.gene24829:CDS |
| CATTCAACTCACTCATGGAA+GGG | 0.509870 | 8.4:+9422307 | None:intergenic |
| GCTCATTCCCGCATCGCGAT+CGG | 0.510279 | 8.4:-9414168 | MS.gene24829:CDS |
| TCTTGTAGGAGAAAGTTACT+TGG | 0.510737 | 8.4:-9415707 | MS.gene24829:CDS |
| GAACTCAAACTGTCATTAGC+AGG | 0.510770 | 8.4:+9416774 | None:intergenic |
| AGGGGTTTCTTTCGGTGTTG+AGG | 0.511421 | 8.4:-9415398 | MS.gene24829:CDS |
| TGGAGAGTTGGAGCCTTACA+AGG | 0.512953 | 8.4:-9419816 | MS.gene24829:CDS |
| ATATGCGAGTATTACCGGAT+TGG | 0.514796 | 8.4:-9418879 | MS.gene24829:CDS |
| AAAGCTCTGAATCGTCCTGC+AGG | 0.515801 | 8.4:-9419604 | MS.gene24829:intron |
| CACTATCTTCAGAAATTCTA+GGG | 0.517423 | 8.4:+9419867 | None:intergenic |
| CACTATCTTCAGAAATTGTA+CGG | 0.519878 | 8.4:+9420014 | None:intergenic |
| CCTACAGATTGGAGCTGCAA+AGG | 0.520536 | 8.4:-9415418 | MS.gene24829:intron |
| GAGAAGCTGACGAAGAGCGT+TGG | 0.520783 | 8.4:-9420766 | MS.gene24829:CDS |
| TTCTCTCCTTATGAGACTGC+AGG | 0.521189 | 8.4:+9420251 | None:intergenic |
| TCACTATCTTCAGAAACTGT+AGG | 0.523218 | 8.4:+9420307 | None:intergenic |
| TCTTTCTGTCTTGGCAGTTG+TGG | 0.523888 | 8.4:+9415763 | None:intergenic |
| CTTTCTTTCTGTTTCCCTGG+AGG | 0.524994 | 8.4:-9422960 | MS.gene24829:CDS |
| CATCTACCATTAGGTTCTCC+GGG | 0.525128 | 8.4:+9420131 | None:intergenic |
| TCGCGATCGGCCTTTCATGA+AGG | 0.527167 | 8.4:-9414155 | MS.gene24829:intron |
| AGCGTAGAAGCCAAAGAACC+TGG | 0.528564 | 8.4:-9420331 | MS.gene24829:CDS |
| TGAAGATAGTGATGGTCACC+AGG | 0.528788 | 8.4:-9420296 | MS.gene24829:CDS |
| GTATGAATGCACAGAAGTGC+AGG | 0.531431 | 8.4:-9415668 | MS.gene24829:intron |
| TTTGTTGATAGGAAATCTGA+TGG | 0.531845 | 8.4:-9423307 | MS.gene24829:CDS |
| ATCGTCATTTGATTGTACAT+TGG | 0.532247 | 8.4:+9415329 | None:intergenic |
| CTGATAAAACTGAAGCAGAA+AGG | 0.533787 | 8.4:+9416831 | None:intergenic |
| CAACAACCATTAGGTTCTCC+GGG | 0.534602 | 8.4:+9420422 | None:intergenic |
| GGTTGATGCCAACAGGAACC+AGG | 0.534661 | 8.4:-9416196 | MS.gene24829:intron |
| AAGAAATAAACCTTCATGAA+AGG | 0.534930 | 8.4:+9414145 | None:intergenic |
| GAGAGTGTGTTGGTGAGTGA+TGG | 0.535318 | 8.4:-9420682 | MS.gene24829:CDS |
| TGATATTCTTGAATGGGCCA+TGG | 0.535501 | 8.4:-9419016 | MS.gene24829:intron |
| CAAGTTACTAAATTGTTCAA+GGG | 0.538937 | 8.4:-9423706 | MS.gene24829:CDS |
| CATCAACTATTAGGTTCTCA+GGG | 0.539193 | 8.4:+9420569 | None:intergenic |
| TCGTAGAACCCGATTGAGCA+TGG | 0.539893 | 8.4:+9412744 | None:intergenic |
| TAGGAGAAAGTTACTTGGGA+AGG | 0.540092 | 8.4:-9415702 | MS.gene24829:CDS |
| CCGGAGAACCAAATAGTTGA+CGG | 0.542247 | 8.4:-9419983 | MS.gene24829:CDS |
| CACTATCTTCAGAAACTGTA+GGG | 0.542555 | 8.4:+9420308 | None:intergenic |
| CTATCAGTTTCATGCTCAGT+AGG | 0.542900 | 8.4:+9420061 | None:intergenic |
| TTACCGAGCATGAAATTGAT+AGG | 0.542943 | 8.4:-9419766 | MS.gene24829:CDS |
| TCATTCAACTCACTCATGGA+AGG | 0.545224 | 8.4:+9422306 | None:intergenic |
| ATTCAACTCACTCATGGAAG+GGG | 0.545368 | 8.4:+9422308 | None:intergenic |
| TGAAGATAGTGATGGTCACC+CGG | 0.546321 | 8.4:-9420002 | MS.gene24829:CDS |
| TGAAGATAGTGATGGTCACC+CGG | 0.546321 | 8.4:-9420440 | MS.gene24829:CDS |
| TGGTCACCCGGAGAACCTAA+TGG | 0.548219 | 8.4:-9420428 | MS.gene24829:CDS |
| CATCAACCATTAGGTTCACC+TGG | 0.548263 | 8.4:+9420278 | None:intergenic |
| AGAACCCAATATTGAACCTC+AGG | 0.548327 | 8.4:-9423236 | MS.gene24829:intron |
| TCTTCAGAAACTGTAGGGCC+AGG | 0.548762 | 8.4:+9420313 | None:intergenic |
| GATAGGAAATCTGATGGATC+TGG | 0.549328 | 8.4:-9423301 | MS.gene24829:CDS |
| CATCGATAAGGCTGGATTAG+CGG | 0.549735 | 8.4:+9416803 | None:intergenic |
| TGCAATGGTTGATGGAGAGT+TGG | 0.550418 | 8.4:-9419828 | MS.gene24829:CDS |
| GTAGCATCTTCACTCAAGAA+AGG | 0.552814 | 8.4:-9420792 | MS.gene24829:intron |
| CTTGTAGGAGAAAGTTACTT+GGG | 0.552826 | 8.4:-9415706 | MS.gene24829:CDS |
| CGGAGAACCAAATAGTTGAC+GGG | 0.553446 | 8.4:-9419982 | MS.gene24829:CDS |
| AAGGGATGCAGGTGCTCCAA+AGG | 0.554651 | 8.4:+9418822 | None:intergenic |
| ATATAATTTGTAGTGGTCCA+TGG | 0.555439 | 8.4:+9418898 | None:intergenic |
| GGCACAGAACACTCTTGCGA+TGG | 0.558151 | 8.4:-9418567 | MS.gene24829:CDS |
| CAGGAGAGGCAAAATAGCAT+GGG | 0.558806 | 8.4:+9419643 | None:intergenic |
| CCAATAAAGAATTAGAAGAT+CGG | 0.559196 | 8.4:-9419673 | MS.gene24829:CDS |
| GCAGGAGAGGCAAAATAGCA+TGG | 0.562597 | 8.4:+9419642 | None:intergenic |
| TACTGAGCATGAAACTGATA+GGG | 0.562704 | 8.4:-9420059 | MS.gene24829:CDS |
| GAACACTCTTGCGATGGAAG+AGG | 0.565276 | 8.4:-9418561 | MS.gene24829:CDS |
| AAATGTAAGTGATGAACCCC+TGG | 0.565984 | 8.4:+9418848 | None:intergenic |
| TGTTCACCCGGAGAACCTAA+TGG | 0.567739 | 8.4:-9420137 | MS.gene24829:CDS |
| GTAGTAGTAGCCAACTTCAA+AGG | 0.569033 | 8.4:-9423738 | MS.gene24829:CDS |
| AGTACGACACAGGACTGTCC+CGG | 0.573443 | 8.4:+9418782 | None:intergenic |
| AACACAAACCTGGTTCCTGT+TGG | 0.574238 | 8.4:+9416188 | None:intergenic |
| AAAGTTTACCAATGTTATTG+TGG | 0.574396 | 8.4:-9416217 | MS.gene24829:CDS |
| ATACTCACTATATAAGGGAC+AGG | 0.574732 | 8.4:+9416619 | None:intergenic |
| GCATACGTTTCATGCTCAGT+AGG | 0.575249 | 8.4:+9420208 | None:intergenic |
| GGCATCAACCACAATAACAT+TGG | 0.578806 | 8.4:+9416209 | None:intergenic |
| TGGTCACCAGGTGAACCTAA+TGG | 0.579355 | 8.4:-9420284 | MS.gene24829:CDS |
| GGATGGTCTATGATATTCCA+AGG | 0.580650 | 8.4:+9418803 | None:intergenic |
| CGCTAATCCAGCCTTATCGA+TGG | 0.586062 | 8.4:-9416802 | MS.gene24829:CDS |
| CGGATTGGTTGTCCTTCCAG+GGG | 0.587875 | 8.4:-9418864 | MS.gene24829:CDS |
| GGTAAATTGAGAAGTTCACT+TGG | 0.592657 | 8.4:-9423280 | MS.gene24829:CDS |
| ACGACTGCCTCTTAAATGCA+AGG | 0.592811 | 8.4:-9422988 | MS.gene24829:CDS |
| TATGATGAAGAAAACTCGGA+GGG | 0.596061 | 8.4:-9420712 | MS.gene24829:CDS |
| ATGCTTTCGAACAGCCGTGA+GGG | 0.596972 | 8.4:+9423382 | None:intergenic |
| AAAGTTACTTGGGAAGGAGA+AGG | 0.598925 | 8.4:-9415696 | MS.gene24829:CDS |
| AACACATACCAAATTAGAGC+AGG | 0.601107 | 8.4:+9417576 | None:intergenic |
| ACAGGGTTATAGGTCGTGGA+AGG | 0.601452 | 8.4:-9423407 | MS.gene24829:intron |
| GGTCGTGGAAGGAGCCCTCA+CGG | 0.602693 | 8.4:-9423396 | MS.gene24829:CDS |
| TGGAATATCATAGACCATCC+GGG | 0.603720 | 8.4:-9418800 | MS.gene24829:CDS |
| TGAAGATAGTGATGTTCACC+CGG | 0.604466 | 8.4:-9420149 | MS.gene24829:CDS |
| TACTCGCATATAATTTGTAG+TGG | 0.605532 | 8.4:+9418891 | None:intergenic |
| CTACTGAGTATGAAACTGAC+AGG | 0.610487 | 8.4:-9420498 | MS.gene24829:CDS |
| TCTTCAGAAATTGTACGGCA+AGG | 0.611661 | 8.4:+9420019 | None:intergenic |
| ATAATTTCTGAAGATAGTGA+TGG | 0.616179 | 8.4:-9420448 | MS.gene24829:CDS |
| ACAATTTCTGAAGATAGTGA+TGG | 0.618662 | 8.4:-9420010 | MS.gene24829:CDS |
| ACCTAACCTCCAAGCCTCCA+GGG | 0.619617 | 8.4:+9422946 | None:intergenic |
| GGGAAATTCAGAGAGTGTGT+TGG | 0.623647 | 8.4:-9420692 | MS.gene24829:CDS |
| TAGATATGATGAAGAAAACT+CGG | 0.624639 | 8.4:-9420716 | MS.gene24829:CDS |
| TCTGAAAACATTGTTGGTGA+TGG | 0.625769 | 8.4:-9420595 | MS.gene24829:CDS |
| CATTGGTAATTGTGTGAGAA+CGG | 0.626236 | 8.4:+9415346 | None:intergenic |
| TACATCAGCTAACTCCAGAC+CGG | 0.627142 | 8.4:+9418728 | None:intergenic |
| TTTCATACTGGGAATAACGC+AGG | 0.628198 | 8.4:+9419624 | None:intergenic |
| ATCAACTATTAGGTTCTCAG+GGG | 0.629913 | 8.4:+9420570 | None:intergenic |
| TCAAAAGATGATAACAACAA+TGG | 0.629958 | 8.4:-9423763 | MS.gene24829:CDS |
| CTGTCAGTTTCATACTCAGT+AGG | 0.631983 | 8.4:+9420499 | None:intergenic |
| GTGATGTTCAGGAACTGCAT+AGG | 0.634986 | 8.4:-9423333 | MS.gene24829:CDS |
| ATCAGAGACAGCAATCCAGA+GGG | 0.636699 | 8.4:+9421728 | None:intergenic |
| CGTCTTAAAGAAGAGTGAGA+TGG | 0.636727 | 8.4:+9421692 | None:intergenic |
| ACAGTTTCTGAAGATAGTGA+TGG | 0.638042 | 8.4:-9420304 | MS.gene24829:CDS |
| TACTGGGAATAACGCAGGAG+AGG | 0.649934 | 8.4:+9419629 | None:intergenic |
| CTGTTGAACCAGTACGACAC+AGG | 0.653684 | 8.4:+9418772 | None:intergenic |
| GTATGAAACTGACAGGTCAA+CGG | 0.656919 | 8.4:-9420491 | MS.gene24829:CDS |
| GTAAGTGATGAACCCCTGGA+AGG | 0.657537 | 8.4:+9418852 | None:intergenic |
| ATGAAAGGCCGATCGCGATG+CGG | 0.666016 | 8.4:+9414160 | None:intergenic |
| ATATGATGAAGAAAACTCGG+AGG | 0.668194 | 8.4:-9420713 | MS.gene24829:CDS |
| AATCAGAGACAGCAATCCAG+AGG | 0.670398 | 8.4:+9421727 | None:intergenic |
| CGACACAGGACTGTCCCGGA+TGG | 0.670958 | 8.4:+9418786 | None:intergenic |
| TGGTCATCTGGAGAATGCAA+TGG | 0.674983 | 8.4:-9419843 | MS.gene24829:CDS |
| AGAATTTCTGAAGATAGTGG+TGG | 0.678763 | 8.4:-9419863 | MS.gene24829:CDS |
| CATGCTTTCGAACAGCCGTG+AGG | 0.679456 | 8.4:+9423381 | None:intergenic |
| GATGGTCTATGATATTCCAA+GGG | 0.684841 | 8.4:+9418804 | None:intergenic |
| AATTTGCAGGAAAATGACCA+TGG | 0.689684 | 8.4:-9418915 | MS.gene24829:intron |
| TACAGATTGGAGCTGCAAAG+GGG | 0.690826 | 8.4:-9415416 | MS.gene24829:intron |
| AGGAGAGGCAAAATAGCATG+GGG | 0.701722 | 8.4:+9419644 | None:intergenic |
| TCAACTATTAGGTTCTCAGG+GGG | 0.705304 | 8.4:+9420571 | None:intergenic |
| TAATAGTTGATGGAGAGCTG+CGG | 0.708549 | 8.4:-9420558 | MS.gene24829:CDS |
| AGTGATGGAATAATAGAACA+CGG | 0.710993 | 8.4:-9420667 | MS.gene24829:CDS |
| ACGGCTGTTCGAAAGCATGG+TGG | 0.718537 | 8.4:-9423377 | MS.gene24829:CDS |
| ATTGACGCTTGAGAGCAGTG+AGG | 0.768293 | 8.4:+9423638 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9412691 | 9423848 | 9412691 | ID=MS.gene24829 |
| chr8.4 | mRNA | 9412691 | 9423848 | 9412691 | ID=MS.gene24829.t1;Parent=MS.gene24829 |
| chr8.4 | exon | 9423609 | 9423848 | 9423609 | ID=MS.gene24829.t1.exon1;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9423609 | 9423848 | 9423609 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9423237 | 9423425 | 9423237 | ID=MS.gene24829.t1.exon2;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9423237 | 9423425 | 9423237 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9422953 | 9423036 | 9422953 | ID=MS.gene24829.t1.exon3;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9422953 | 9423036 | 9422953 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9422290 | 9422349 | 9422290 | ID=MS.gene24829.t1.exon4;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9422290 | 9422349 | 9422290 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9421932 | 9421976 | 9421932 | ID=MS.gene24829.t1.exon5;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9421932 | 9421976 | 9421932 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9421611 | 9421810 | 9421611 | ID=MS.gene24829.t1.exon6;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9421611 | 9421810 | 9421611 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9419605 | 9420810 | 9419605 | ID=MS.gene24829.t1.exon7;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9419605 | 9420810 | 9419605 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9419017 | 9419143 | 9419017 | ID=MS.gene24829.t1.exon8;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9419017 | 9419143 | 9419017 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9418731 | 9418928 | 9418731 | ID=MS.gene24829.t1.exon9;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9418731 | 9418928 | 9418731 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9418502 | 9418588 | 9418502 | ID=MS.gene24829.t1.exon10;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9418502 | 9418588 | 9418502 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9417585 | 9417647 | 9417585 | ID=MS.gene24829.t1.exon11;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9417585 | 9417647 | 9417585 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9416784 | 9416858 | 9416784 | ID=MS.gene24829.t1.exon12;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9416784 | 9416858 | 9416784 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9416627 | 9416677 | 9416627 | ID=MS.gene24829.t1.exon13;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9416627 | 9416677 | 9416627 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9416197 | 9416274 | 9416197 | ID=MS.gene24829.t1.exon14;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9416197 | 9416274 | 9416197 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9415669 | 9415803 | 9415669 | ID=MS.gene24829.t1.exon15;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9415669 | 9415803 | 9415669 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9415326 | 9415433 | 9415326 | ID=MS.gene24829.t1.exon16;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9415326 | 9415433 | 9415326 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9414156 | 9414221 | 9414156 | ID=MS.gene24829.t1.exon17;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9414156 | 9414221 | 9414156 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| chr8.4 | exon | 9412691 | 9412768 | 9412691 | ID=MS.gene24829.t1.exon18;Parent=MS.gene24829.t1 |
| chr8.4 | CDS | 9412691 | 9412768 | 9412691 | ID=cds.MS.gene24829.t1;Parent=MS.gene24829.t1 |
| Gene Sequence |
| Protein sequence |
