Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24834.t1 | RHN64587.1 | 86.2 | 218 | 26 | 1 | 1 | 218 | 1 | 214 | 8.00E-100 | 373.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24834.t1 | Q9SYD8 | 40.5 | 220 | 120 | 5 | 1 | 219 | 1 | 210 | 1.4e-36 | 154.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24834.t1 | A0A072USZ1 | 86.2 | 218 | 26 | 1 | 1 | 218 | 2 | 215 | 5.8e-100 | 373.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051812 | MS.gene24834 | 0.885708 | 6.44E-72 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24834.t1 | MTR_4g125560 | 86.239 | 218 | 26 | 1 | 1 | 218 | 2 | 215 | 8.01e-127 | 357 |
| MS.gene24834.t1 | MTR_4g125530 | 45.588 | 204 | 101 | 6 | 5 | 205 | 52 | 248 | 4.08e-58 | 184 |
| MS.gene24834.t1 | MTR_0121s0060 | 37.900 | 219 | 117 | 6 | 5 | 213 | 52 | 261 | 8.46e-45 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24834.t1 | AT1G51360 | 40.455 | 220 | 120 | 5 | 1 | 219 | 1 | 210 | 1.07e-47 | 156 |
| MS.gene24834.t1 | AT2G31670 | 38.532 | 218 | 115 | 6 | 4 | 213 | 48 | 254 | 2.58e-47 | 157 |
Find 57 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGATTCTGATGAGAAATT+TGG | 0.119057 | 8.4:+9370329 | MS.gene24834:CDS |
| TCACATGAATGATAAGATTA+AGG | 0.311885 | 8.4:+9370358 | MS.gene24834:CDS |
| CTTCAAGTTGAAGGAGGGTT+TGG | 0.321534 | 8.4:+9370148 | MS.gene24834:CDS |
| AGAGCCACTTCACTTAACTA+TGG | 0.332780 | 8.4:+9369887 | MS.gene24834:CDS |
| CTGATTCTGATGAGAAATTT+GGG | 0.334720 | 8.4:+9370330 | MS.gene24834:CDS |
| TCGAGCATGTGGTGTTGTTC+AGG | 0.352637 | 8.4:+9369793 | MS.gene24834:CDS |
| TCCAGGTTTAACCGAATTGC+AGG | 0.367963 | 8.4:+9370304 | MS.gene24834:CDS |
| TTCAAGTTGAAGGAGGGTTT+GGG | 0.376642 | 8.4:+9370149 | MS.gene24834:CDS |
| TTCTCTCCTGGTAGAGCTAA+AGG | 0.386910 | 8.4:+9370254 | MS.gene24834:CDS |
| ATGAAACCATGGCTCTTGAT+TGG | 0.394542 | 8.4:+9370063 | MS.gene24834:CDS |
| ACTGAAAGATGAAAGGGAAT+TGG | 0.400879 | 8.4:-9369934 | None:intergenic |
| CTGGACACTGTACTGGTCCT+TGG | 0.405106 | 8.4:+9370386 | MS.gene24834:CDS |
| AGAGCTAAAGGATTCTCTAT+TGG | 0.420200 | 8.4:+9370266 | MS.gene24834:CDS |
| AGAATCAGCTTCCTGCAATT+CGG | 0.430055 | 8.4:-9370315 | None:intergenic |
| TGGACACTGTACTGGTCCTT+GGG | 0.441862 | 8.4:+9370387 | MS.gene24834:CDS |
| GCCATGGTTTCATCAACTAT+AGG | 0.450016 | 8.4:-9370053 | None:intergenic |
| AAAGCTGTTCACTTTAACTG+TGG | 0.466155 | 8.4:+9370224 | MS.gene24834:CDS |
| AGTTGTGTTCTTCAAGTTGA+AGG | 0.472243 | 8.4:+9370139 | MS.gene24834:CDS |
| TCCTGCAATTCGGTTAAACC+TGG | 0.483602 | 8.4:-9370305 | None:intergenic |
| AACCTCGTCAAGATGATGTT+GGG | 0.486659 | 8.4:-9370424 | None:intergenic |
| GGAAAACCTGGACACTGTAC+TGG | 0.488642 | 8.4:+9370379 | MS.gene24834:CDS |
| CGAGGTTCACGGTAAGGACT+TGG | 0.489470 | 8.4:+9370091 | MS.gene24834:CDS |
| GAAAGATGAAAGGGAATTGG+AGG | 0.496529 | 8.4:-9369931 | None:intergenic |
| TGAAACCATGGCTCTTGATT+GGG | 0.498755 | 8.4:+9370064 | MS.gene24834:CDS |
| GATTGGGTCACCGAGGTTCA+CGG | 0.504028 | 8.4:+9370080 | MS.gene24834:CDS |
| TGCAGTGCACCCTACACACT+TGG | 0.533139 | 8.4:+9370010 | MS.gene24834:CDS |
| ACAATCAGCGATTGATTCCA+TGG | 0.534420 | 8.4:+9369833 | MS.gene24834:CDS |
| AAACCTCGTCAAGATGATGT+TGG | 0.534539 | 8.4:-9370425 | None:intergenic |
| AAGCTGTTCACTTTAACTGT+GGG | 0.537942 | 8.4:+9370225 | MS.gene24834:CDS |
| GTGTGTAGGGTGCACTGCAT+AGG | 0.548626 | 8.4:-9370006 | None:intergenic |
| TGATAAGATTAAGGAAAACC+TGG | 0.551255 | 8.4:+9370367 | MS.gene24834:CDS |
| GAGCCACTTCACTTAACTAT+GGG | 0.556132 | 8.4:+9369888 | MS.gene24834:CDS |
| GGTCACCGAGGTTCACGGTA+AGG | 0.556416 | 8.4:+9370085 | MS.gene24834:CDS |
| TGATGACAGCCAAGTGTGTA+GGG | 0.562820 | 8.4:-9370019 | None:intergenic |
| AGTGAAACTGAAAGATGAAA+GGG | 0.568571 | 8.4:-9369940 | None:intergenic |
| GTGTTCTTCAAGTTGAAGGA+GGG | 0.573144 | 8.4:+9370143 | MS.gene24834:CDS |
| ATGGAATCAATCGCTGATTG+TGG | 0.574412 | 8.4:-9369831 | None:intergenic |
| TCACTTAACTATGGGTCCGT+TGG | 0.580187 | 8.4:+9369896 | MS.gene24834:CDS |
| GGAATTCACTTGCTTCACCA+TGG | 0.585437 | 8.4:-9369850 | None:intergenic |
| GAGAATCCTTTAGCTCTACC+AGG | 0.585556 | 8.4:-9370260 | None:intergenic |
| TGTGTTCTTCAAGTTGAAGG+AGG | 0.590785 | 8.4:+9370142 | MS.gene24834:CDS |
| CAAGGACCAGTACAGTGTCC+AGG | 0.590998 | 8.4:-9370385 | None:intergenic |
| GAGTGAAACTGAAAGATGAA+AGG | 0.600199 | 8.4:-9369941 | None:intergenic |
| GTTATCAATAATCGAGCATG+TGG | 0.614479 | 8.4:+9369782 | MS.gene24834:CDS |
| ATAGGCATGGAGATCTTCGT+TGG | 0.619096 | 8.4:-9369988 | None:intergenic |
| TTGATGACAGCCAAGTGTGT+AGG | 0.625608 | 8.4:-9370020 | None:intergenic |
| GCCTATAGTTGATGAAACCA+TGG | 0.626475 | 8.4:+9370052 | MS.gene24834:CDS |
| GGACCCATAGTTAAGTGAAG+TGG | 0.629855 | 8.4:-9369891 | None:intergenic |
| GGCTATGCATCACATACCCA+AGG | 0.633264 | 8.4:-9370403 | None:intergenic |
| TAGGGTGCACTGCATAGGCA+TGG | 0.636624 | 8.4:-9370001 | None:intergenic |
| GAGGATTGAATACTTACCAA+CGG | 0.640198 | 8.4:-9369912 | None:intergenic |
| GGTGACCCAATCAAGAGCCA+TGG | 0.645022 | 8.4:-9370069 | None:intergenic |
| GGCTCTTGATTGGGTCACCG+AGG | 0.651959 | 8.4:+9370073 | MS.gene24834:CDS |
| CAAGTCCTTACCGTGAACCT+CGG | 0.655186 | 8.4:-9370090 | None:intergenic |
| AAGGACTTGGTTCTACAACA+TGG | 0.659065 | 8.4:+9370104 | MS.gene24834:CDS |
| TGGCTCTTCAAGAGAGACAA+GGG | 0.659348 | 8.4:-9369871 | None:intergenic |
| GTGGCTCTTCAAGAGAGACA+AGG | 0.665275 | 8.4:-9369872 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCACATGAATGATAAGATTA+AGG | + | chr8.4:9370358-9370377 | MS.gene24834:CDS | 25.0% |
| AGTGAAACTGAAAGATGAAA+GGG | - | chr8.4:9369943-9369962 | None:intergenic | 30.0% | |
| CTGATTCTGATGAGAAATTT+GGG | + | chr8.4:9370330-9370349 | MS.gene24834:CDS | 30.0% | |
| TGATAAGATTAAGGAAAACC+TGG | + | chr8.4:9370367-9370386 | MS.gene24834:CDS | 30.0% | |
| !! | AGTGAAAAATGAGGTTTTGA+AGG | + | chr8.4:9370178-9370197 | MS.gene24834:CDS | 30.0% |
| !!! | GTGAAAAATGAGGTTTTGAA+GGG | + | chr8.4:9370179-9370198 | MS.gene24834:CDS | 30.0% |
| AAAGCTGTTCACTTTAACTG+TGG | + | chr8.4:9370224-9370243 | MS.gene24834:CDS | 35.0% | |
| AAGCTGTTCACTTTAACTGT+GGG | + | chr8.4:9370225-9370244 | MS.gene24834:CDS | 35.0% | |
| ACTGAAAGATGAAAGGGAAT+TGG | - | chr8.4:9369937-9369956 | None:intergenic | 35.0% | |
| AGAGCTAAAGGATTCTCTAT+TGG | + | chr8.4:9370266-9370285 | MS.gene24834:CDS | 35.0% | |
| GAGGATTGAATACTTACCAA+CGG | - | chr8.4:9369915-9369934 | None:intergenic | 35.0% | |
| GAGTGAAACTGAAAGATGAA+AGG | - | chr8.4:9369944-9369963 | None:intergenic | 35.0% | |
| GTTATCAATAATCGAGCATG+TGG | + | chr8.4:9369782-9369801 | MS.gene24834:CDS | 35.0% | |
| TAAACCTGGAAAAACTGCAA+CGG | - | chr8.4:9370294-9370313 | None:intergenic | 35.0% | |
| ! | GCTGATTCTGATGAGAAATT+TGG | + | chr8.4:9370329-9370348 | MS.gene24834:CDS | 35.0% |
| !! | AGTTGTGTTCTTCAAGTTGA+AGG | + | chr8.4:9370139-9370158 | MS.gene24834:CDS | 35.0% |
| AGAATCAGCTTCCTGCAATT+CGG | - | chr8.4:9370318-9370337 | None:intergenic | 40.0% | |
| AGAGCCACTTCACTTAACTA+TGG | + | chr8.4:9369887-9369906 | MS.gene24834:CDS | 40.0% | |
| ATGAAACCATGGCTCTTGAT+TGG | + | chr8.4:9370063-9370082 | MS.gene24834:CDS | 40.0% | |
| GAAAGATGAAAGGGAATTGG+AGG | - | chr8.4:9369934-9369953 | None:intergenic | 40.0% | |
| GAGCCACTTCACTTAACTAT+GGG | + | chr8.4:9369888-9369907 | MS.gene24834:CDS | 40.0% | |
| GCCATGGTTTCATCAACTAT+AGG | - | chr8.4:9370056-9370075 | None:intergenic | 40.0% | |
| GGGAGAAGAAGTGAAAAATG+AGG | + | chr8.4:9370169-9370188 | MS.gene24834:CDS | 40.0% | |
| TGAAACCATGGCTCTTGATT+GGG | + | chr8.4:9370064-9370083 | MS.gene24834:CDS | 40.0% | |
| TGTGGGAAAAATTTCTCTCC+TGG | + | chr8.4:9370242-9370261 | MS.gene24834:CDS | 40.0% | |
| ! | ACAATCAGCGATTGATTCCA+TGG | + | chr8.4:9369833-9369852 | MS.gene24834:CDS | 40.0% |
| ! | ATGGAATCAATCGCTGATTG+TGG | - | chr8.4:9369834-9369853 | None:intergenic | 40.0% |
| !! | AAGGACTTGGTTCTACAACA+TGG | + | chr8.4:9370104-9370123 | MS.gene24834:CDS | 40.0% |
| !! | GCCTATAGTTGATGAAACCA+TGG | + | chr8.4:9370052-9370071 | MS.gene24834:CDS | 40.0% |
| !! | GTGTTCTTCAAGTTGAAGGA+GGG | + | chr8.4:9370143-9370162 | MS.gene24834:CDS | 40.0% |
| !! | TGTGTTCTTCAAGTTGAAGG+AGG | + | chr8.4:9370142-9370161 | MS.gene24834:CDS | 40.0% |
| !! | TTCAAGTTGAAGGAGGGTTT+GGG | + | chr8.4:9370149-9370168 | MS.gene24834:CDS | 40.0% |
| !!! | GAGGTTTTGAAGGGTATAAG+TGG | + | chr8.4:9370188-9370207 | MS.gene24834:CDS | 40.0% |
| ATAGGCATGGAGATCTTCGT+TGG | - | chr8.4:9369991-9370010 | None:intergenic | 45.0% | |
| GGAATTCACTTGCTTCACCA+TGG | - | chr8.4:9369853-9369872 | None:intergenic | 45.0% | |
| GGACCCATAGTTAAGTGAAG+TGG | - | chr8.4:9369894-9369913 | None:intergenic | 45.0% | |
| TCACTTAACTATGGGTCCGT+TGG | + | chr8.4:9369896-9369915 | MS.gene24834:CDS | 45.0% | |
| TCCAGGTTTAACCGAATTGC+AGG | + | chr8.4:9370304-9370323 | MS.gene24834:CDS | 45.0% | |
| TCCTGCAATTCGGTTAAACC+TGG | - | chr8.4:9370308-9370327 | None:intergenic | 45.0% | |
| TGATGACAGCCAAGTGTGTA+GGG | - | chr8.4:9370022-9370041 | None:intergenic | 45.0% | |
| TGGCTCTTCAAGAGAGACAA+GGG | - | chr8.4:9369874-9369893 | None:intergenic | 45.0% | |
| TTCTCTCCTGGTAGAGCTAA+AGG | + | chr8.4:9370254-9370273 | MS.gene24834:CDS | 45.0% | |
| ! | TTGATGACAGCCAAGTGTGT+AGG | - | chr8.4:9370023-9370042 | None:intergenic | 45.0% |
| !! | CTTCAAGTTGAAGGAGGGTT+TGG | + | chr8.4:9370148-9370167 | MS.gene24834:CDS | 45.0% |
| !! | GAGAATCCTTTAGCTCTACC+AGG | - | chr8.4:9370263-9370282 | None:intergenic | 45.0% |
| CAAGTCCTTACCGTGAACCT+CGG | - | chr8.4:9370093-9370112 | None:intergenic | 50.0% | |
| GGAAAACCTGGACACTGTAC+TGG | + | chr8.4:9370379-9370398 | MS.gene24834:CDS | 50.0% | |
| GGCTATGCATCACATACCCA+AGG | - | chr8.4:9370406-9370425 | None:intergenic | 50.0% | |
| GTGGCTCTTCAAGAGAGACA+AGG | - | chr8.4:9369875-9369894 | None:intergenic | 50.0% | |
| ! | GGTTCCGTTGCAGTTTTTCC+AGG | + | chr8.4:9370287-9370306 | MS.gene24834:CDS | 50.0% |
| ! | TGGACACTGTACTGGTCCTT+GGG | + | chr8.4:9370387-9370406 | MS.gene24834:CDS | 50.0% |
| !! | TCGAGCATGTGGTGTTGTTC+AGG | + | chr8.4:9369793-9369812 | MS.gene24834:CDS | 50.0% |
| CAAGGACCAGTACAGTGTCC+AGG | - | chr8.4:9370388-9370407 | None:intergenic | 55.0% | |
| CGAGGTTCACGGTAAGGACT+TGG | + | chr8.4:9370091-9370110 | MS.gene24834:CDS | 55.0% | |
| GATTGGGTCACCGAGGTTCA+CGG | + | chr8.4:9370080-9370099 | MS.gene24834:CDS | 55.0% | |
| GGTGACCCAATCAAGAGCCA+TGG | - | chr8.4:9370072-9370091 | None:intergenic | 55.0% | |
| GTGTGTAGGGTGCACTGCAT+AGG | - | chr8.4:9370009-9370028 | None:intergenic | 55.0% | |
| TAGGGTGCACTGCATAGGCA+TGG | - | chr8.4:9370004-9370023 | None:intergenic | 55.0% | |
| TGCAGTGCACCCTACACACT+TGG | + | chr8.4:9370010-9370029 | MS.gene24834:CDS | 55.0% | |
| ! | CTGGACACTGTACTGGTCCT+TGG | + | chr8.4:9370386-9370405 | MS.gene24834:CDS | 55.0% |
| GGCTCTTGATTGGGTCACCG+AGG | + | chr8.4:9370073-9370092 | MS.gene24834:CDS | 60.0% | |
| GGTCACCGAGGTTCACGGTA+AGG | + | chr8.4:9370085-9370104 | MS.gene24834:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9369780 | 9370439 | 9369780 | ID=MS.gene24834 |
| chr8.4 | mRNA | 9369780 | 9370439 | 9369780 | ID=MS.gene24834.t1;Parent=MS.gene24834 |
| chr8.4 | exon | 9369780 | 9370439 | 9369780 | ID=MS.gene24834.t1.exon1;Parent=MS.gene24834.t1 |
| chr8.4 | CDS | 9369780 | 9370439 | 9369780 | ID=cds.MS.gene24834.t1;Parent=MS.gene24834.t1 |
| Gene Sequence |
| Protein sequence |
