Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051812.t1 | RHN64587.1 | 86.2 | 218 | 26 | 1 | 1 | 218 | 1 | 214 | 2.30E-99 | 371.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051812.t1 | Q9SYD8 | 41.0 | 212 | 116 | 4 | 1 | 211 | 1 | 204 | 1.4e-36 | 154.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051812.t1 | A0A072USZ1 | 86.2 | 218 | 26 | 1 | 1 | 218 | 2 | 215 | 1.7e-99 | 371.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051812 | MS.gene24834 | 0.885708 | 6.44E-72 | -1.69E-46 |
| MS.gene051812 | MS.gene55651 | 0.801897 | 7.25E-49 | -1.69E-46 |
| MS.gene051812 | MS.gene87921 | 0.801084 | 1.06E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051812.t1 | MTR_4g125560 | 86.239 | 218 | 26 | 1 | 1 | 218 | 2 | 215 | 1.15e-126 | 357 |
| MS.gene051812.t1 | MTR_4g125530 | 45.854 | 205 | 99 | 6 | 5 | 205 | 52 | 248 | 7.52e-59 | 186 |
| MS.gene051812.t1 | MTR_0121s0060 | 38.356 | 219 | 116 | 7 | 5 | 213 | 52 | 261 | 1.37e-44 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051812.t1 | AT2G31670 | 38.991 | 218 | 114 | 6 | 4 | 213 | 48 | 254 | 3.31e-48 | 159 |
| MS.gene051812.t1 | AT1G51360 | 40.930 | 215 | 112 | 5 | 1 | 211 | 1 | 204 | 1.04e-47 | 156 |
Find 56 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGATTCTGATGAGAAATT+TGG | 0.119057 | 8.2:+7189217 | MS.gene051812:CDS |
| TCACATGAATGATAAGATTA+AGG | 0.311885 | 8.2:+7189246 | MS.gene051812:CDS |
| CTTCAAGTTGAAGGAGGGTT+TGG | 0.321534 | 8.2:+7189036 | MS.gene051812:CDS |
| AGAGCCACTTCACTTAACTA+TGG | 0.332780 | 8.2:+7188775 | MS.gene051812:CDS |
| CTGATTCTGATGAGAAATTT+GGG | 0.334720 | 8.2:+7189218 | MS.gene051812:CDS |
| TCGAGCATGTGGTGTTGTTC+AGG | 0.352637 | 8.2:+7188681 | MS.gene051812:CDS |
| TGATAAGATTAAGGAAAATC+TGG | 0.365828 | 8.2:+7189255 | MS.gene051812:CDS |
| TCCAGGTTTAACCGAATTGC+AGG | 0.367963 | 8.2:+7189192 | MS.gene051812:CDS |
| TTCAAGTTGAAGGAGGGTTT+GGG | 0.376642 | 8.2:+7189037 | MS.gene051812:CDS |
| TTCTCTCCTGGTAGAGCTAA+AGG | 0.386910 | 8.2:+7189142 | MS.gene051812:CDS |
| ATGAAACCATGGCTCTTGAT+TGG | 0.394542 | 8.2:+7188951 | MS.gene051812:CDS |
| ACTGAAAGATGAAAGGGAAT+TGG | 0.400879 | 8.2:-7188822 | None:intergenic |
| CTGGACACTGTACTGGTCCT+TGG | 0.413853 | 8.2:+7189274 | MS.gene051812:CDS |
| AGAGCTAAAGGATTCTCTAT+TGG | 0.420200 | 8.2:+7189154 | MS.gene051812:CDS |
| AGAATCAGCTTCCTGCAATT+CGG | 0.430055 | 8.2:-7189203 | None:intergenic |
| GCCATGGTTTCATCAACTAT+AGG | 0.450016 | 8.2:-7188941 | None:intergenic |
| TGGACACTGTACTGGTCCTT+GGG | 0.463405 | 8.2:+7189275 | MS.gene051812:CDS |
| ATATCTCATCAGAATGATGT+TGG | 0.470355 | 8.2:-7189313 | None:intergenic |
| AGTTGTGTTCTTCAAGTTGA+AGG | 0.472243 | 8.2:+7189027 | MS.gene051812:CDS |
| GGAAAATCTGGACACTGTAC+TGG | 0.477993 | 8.2:+7189267 | MS.gene051812:CDS |
| TCCTGCAATTCGGTTAAACC+TGG | 0.483602 | 8.2:-7189193 | None:intergenic |
| CGAGGTTCACGGTAAGGACT+TGG | 0.489470 | 8.2:+7188979 | MS.gene051812:CDS |
| GAAAGATGAAAGGGAATTGG+AGG | 0.496529 | 8.2:-7188819 | None:intergenic |
| TGAAACCATGGCTCTTGATT+GGG | 0.498755 | 8.2:+7188952 | MS.gene051812:CDS |
| GATTGGGTCACCGAGGTTCA+CGG | 0.504028 | 8.2:+7188968 | MS.gene051812:CDS |
| TATCTCATCAGAATGATGTT+GGG | 0.526285 | 8.2:-7189312 | None:intergenic |
| TGCAGTGCACCCTACACACT+TGG | 0.533139 | 8.2:+7188898 | MS.gene051812:CDS |
| ACAATCAGCGATTGATTCCA+TGG | 0.534420 | 8.2:+7188721 | MS.gene051812:CDS |
| GTGTGTAGGGTGCACTGCAT+AGG | 0.548626 | 8.2:-7188894 | None:intergenic |
| GAGCCACTTCACTTAACTAT+GGG | 0.556132 | 8.2:+7188776 | MS.gene051812:CDS |
| GGTCACCGAGGTTCACGGTA+AGG | 0.556416 | 8.2:+7188973 | MS.gene051812:CDS |
| TGATGACAGCCAAGTGTGTA+GGG | 0.562820 | 8.2:-7188907 | None:intergenic |
| AGTGAAACTGAAAGATGAAA+GGG | 0.568571 | 8.2:-7188828 | None:intergenic |
| AAGCTGTTCAGTTTAACTGT+GGG | 0.568912 | 8.2:+7189113 | MS.gene051812:CDS |
| GTGTTCTTCAAGTTGAAGGA+GGG | 0.573144 | 8.2:+7189031 | MS.gene051812:CDS |
| ATGGAATCAATCGCTGATTG+TGG | 0.574412 | 8.2:-7188719 | None:intergenic |
| TCACTTAACTATGGGTCCGT+TGG | 0.580187 | 8.2:+7188784 | MS.gene051812:CDS |
| GGAATTCACTTGCTTCACCA+TGG | 0.585437 | 8.2:-7188738 | None:intergenic |
| GAGAATCCTTTAGCTCTACC+AGG | 0.585556 | 8.2:-7189148 | None:intergenic |
| TGTGTTCTTCAAGTTGAAGG+AGG | 0.590785 | 8.2:+7189030 | MS.gene051812:CDS |
| AAAGCTGTTCAGTTTAACTG+TGG | 0.598480 | 8.2:+7189112 | MS.gene051812:CDS |
| GAGTGAAACTGAAAGATGAA+AGG | 0.600199 | 8.2:-7188829 | None:intergenic |
| GTTATCAATAATCGAGCATG+TGG | 0.614479 | 8.2:+7188670 | MS.gene051812:CDS |
| ATAGGCATGGAGATCTTCGT+TGG | 0.619096 | 8.2:-7188876 | None:intergenic |
| TTGATGACAGCCAAGTGTGT+AGG | 0.625608 | 8.2:-7188908 | None:intergenic |
| GCCTATAGTTGATGAAACCA+TGG | 0.626475 | 8.2:+7188940 | MS.gene051812:CDS |
| GGACCCATAGTTAAGTGAAG+TGG | 0.629855 | 8.2:-7188779 | None:intergenic |
| GGCTATGCATCACATACCCA+AGG | 0.633264 | 8.2:-7189291 | None:intergenic |
| TAGGGTGCACTGCATAGGCA+TGG | 0.636624 | 8.2:-7188889 | None:intergenic |
| GAGGATTGAATACTTACCAA+CGG | 0.640198 | 8.2:-7188800 | None:intergenic |
| GGTGACCCAATCAAGAGCCA+TGG | 0.645022 | 8.2:-7188957 | None:intergenic |
| GGCTCTTGATTGGGTCACCG+AGG | 0.651959 | 8.2:+7188961 | MS.gene051812:CDS |
| CAAGTCCTTACCGTGAACCT+CGG | 0.655186 | 8.2:-7188978 | None:intergenic |
| AAGGACTTGGTTCTACAACA+TGG | 0.659065 | 8.2:+7188992 | MS.gene051812:CDS |
| TGGCTCTTCAAGAGAGACAA+GGG | 0.659348 | 8.2:-7188759 | None:intergenic |
| GTGGCTCTTCAAGAGAGACA+AGG | 0.665275 | 8.2:-7188760 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCACATGAATGATAAGATTA+AGG | + | chr8.2:7189246-7189265 | MS.gene051812:CDS | 25.0% |
| ! | TGATAAGATTAAGGAAAATC+TGG | + | chr8.2:7189255-7189274 | MS.gene051812:CDS | 25.0% |
| AGTGAAACTGAAAGATGAAA+GGG | - | chr8.2:7188831-7188850 | None:intergenic | 30.0% | |
| CTGATTCTGATGAGAAATTT+GGG | + | chr8.2:7189218-7189237 | MS.gene051812:CDS | 30.0% | |
| !! | AGTGAAAAATGAGGTTTTGA+AGG | + | chr8.2:7189066-7189085 | MS.gene051812:CDS | 30.0% |
| !!! | GTGAAAAATGAGGTTTTGAA+GGG | + | chr8.2:7189067-7189086 | MS.gene051812:CDS | 30.0% |
| AAAGCTGTTCAGTTTAACTG+TGG | + | chr8.2:7189112-7189131 | MS.gene051812:CDS | 35.0% | |
| AAGCTGTTCAGTTTAACTGT+GGG | + | chr8.2:7189113-7189132 | MS.gene051812:CDS | 35.0% | |
| ACTGAAAGATGAAAGGGAAT+TGG | - | chr8.2:7188825-7188844 | None:intergenic | 35.0% | |
| AGAGCTAAAGGATTCTCTAT+TGG | + | chr8.2:7189154-7189173 | MS.gene051812:CDS | 35.0% | |
| GAGGATTGAATACTTACCAA+CGG | - | chr8.2:7188803-7188822 | None:intergenic | 35.0% | |
| GAGTGAAACTGAAAGATGAA+AGG | - | chr8.2:7188832-7188851 | None:intergenic | 35.0% | |
| GTTATCAATAATCGAGCATG+TGG | + | chr8.2:7188670-7188689 | MS.gene051812:CDS | 35.0% | |
| TAAACCTGGAAAAACTGCAA+CGG | - | chr8.2:7189182-7189201 | None:intergenic | 35.0% | |
| ! | GCTGATTCTGATGAGAAATT+TGG | + | chr8.2:7189217-7189236 | MS.gene051812:CDS | 35.0% |
| !! | AGTTGTGTTCTTCAAGTTGA+AGG | + | chr8.2:7189027-7189046 | MS.gene051812:CDS | 35.0% |
| AGAATCAGCTTCCTGCAATT+CGG | - | chr8.2:7189206-7189225 | None:intergenic | 40.0% | |
| AGAGCCACTTCACTTAACTA+TGG | + | chr8.2:7188775-7188794 | MS.gene051812:CDS | 40.0% | |
| ATGAAACCATGGCTCTTGAT+TGG | + | chr8.2:7188951-7188970 | MS.gene051812:CDS | 40.0% | |
| GAAAGATGAAAGGGAATTGG+AGG | - | chr8.2:7188822-7188841 | None:intergenic | 40.0% | |
| GAGCCACTTCACTTAACTAT+GGG | + | chr8.2:7188776-7188795 | MS.gene051812:CDS | 40.0% | |
| GCCATGGTTTCATCAACTAT+AGG | - | chr8.2:7188944-7188963 | None:intergenic | 40.0% | |
| GGGAGAAGAAGTGAAAAATG+AGG | + | chr8.2:7189057-7189076 | MS.gene051812:CDS | 40.0% | |
| TGAAACCATGGCTCTTGATT+GGG | + | chr8.2:7188952-7188971 | MS.gene051812:CDS | 40.0% | |
| TGTGGGAAAAATTTCTCTCC+TGG | + | chr8.2:7189130-7189149 | MS.gene051812:CDS | 40.0% | |
| ! | ACAATCAGCGATTGATTCCA+TGG | + | chr8.2:7188721-7188740 | MS.gene051812:CDS | 40.0% |
| ! | ATGGAATCAATCGCTGATTG+TGG | - | chr8.2:7188722-7188741 | None:intergenic | 40.0% |
| !! | AAGGACTTGGTTCTACAACA+TGG | + | chr8.2:7188992-7189011 | MS.gene051812:CDS | 40.0% |
| !! | GCCTATAGTTGATGAAACCA+TGG | + | chr8.2:7188940-7188959 | MS.gene051812:CDS | 40.0% |
| !! | GTGTTCTTCAAGTTGAAGGA+GGG | + | chr8.2:7189031-7189050 | MS.gene051812:CDS | 40.0% |
| !! | TGTGTTCTTCAAGTTGAAGG+AGG | + | chr8.2:7189030-7189049 | MS.gene051812:CDS | 40.0% |
| !! | TTCAAGTTGAAGGAGGGTTT+GGG | + | chr8.2:7189037-7189056 | MS.gene051812:CDS | 40.0% |
| !!! | GAGGTTTTGAAGGGTATAAG+TGG | + | chr8.2:7189076-7189095 | MS.gene051812:CDS | 40.0% |
| ATAGGCATGGAGATCTTCGT+TGG | - | chr8.2:7188879-7188898 | None:intergenic | 45.0% | |
| GGAAAATCTGGACACTGTAC+TGG | + | chr8.2:7189267-7189286 | MS.gene051812:CDS | 45.0% | |
| GGAATTCACTTGCTTCACCA+TGG | - | chr8.2:7188741-7188760 | None:intergenic | 45.0% | |
| GGACCCATAGTTAAGTGAAG+TGG | - | chr8.2:7188782-7188801 | None:intergenic | 45.0% | |
| TCACTTAACTATGGGTCCGT+TGG | + | chr8.2:7188784-7188803 | MS.gene051812:CDS | 45.0% | |
| TCCAGGTTTAACCGAATTGC+AGG | + | chr8.2:7189192-7189211 | MS.gene051812:CDS | 45.0% | |
| TCCTGCAATTCGGTTAAACC+TGG | - | chr8.2:7189196-7189215 | None:intergenic | 45.0% | |
| TGATGACAGCCAAGTGTGTA+GGG | - | chr8.2:7188910-7188929 | None:intergenic | 45.0% | |
| TGGCTCTTCAAGAGAGACAA+GGG | - | chr8.2:7188762-7188781 | None:intergenic | 45.0% | |
| TTCTCTCCTGGTAGAGCTAA+AGG | + | chr8.2:7189142-7189161 | MS.gene051812:CDS | 45.0% | |
| ! | TTGATGACAGCCAAGTGTGT+AGG | - | chr8.2:7188911-7188930 | None:intergenic | 45.0% |
| !! | CTTCAAGTTGAAGGAGGGTT+TGG | + | chr8.2:7189036-7189055 | MS.gene051812:CDS | 45.0% |
| !! | GAGAATCCTTTAGCTCTACC+AGG | - | chr8.2:7189151-7189170 | None:intergenic | 45.0% |
| CAAGTCCTTACCGTGAACCT+CGG | - | chr8.2:7188981-7189000 | None:intergenic | 50.0% | |
| GGCTATGCATCACATACCCA+AGG | - | chr8.2:7189294-7189313 | None:intergenic | 50.0% | |
| GTGGCTCTTCAAGAGAGACA+AGG | - | chr8.2:7188763-7188782 | None:intergenic | 50.0% | |
| ! | GGTTCCGTTGCAGTTTTTCC+AGG | + | chr8.2:7189175-7189194 | MS.gene051812:CDS | 50.0% |
| ! | TGGACACTGTACTGGTCCTT+GGG | + | chr8.2:7189275-7189294 | MS.gene051812:CDS | 50.0% |
| !! | TCGAGCATGTGGTGTTGTTC+AGG | + | chr8.2:7188681-7188700 | MS.gene051812:CDS | 50.0% |
| CGAGGTTCACGGTAAGGACT+TGG | + | chr8.2:7188979-7188998 | MS.gene051812:CDS | 55.0% | |
| GATTGGGTCACCGAGGTTCA+CGG | + | chr8.2:7188968-7188987 | MS.gene051812:CDS | 55.0% | |
| GGTGACCCAATCAAGAGCCA+TGG | - | chr8.2:7188960-7188979 | None:intergenic | 55.0% | |
| GTGTGTAGGGTGCACTGCAT+AGG | - | chr8.2:7188897-7188916 | None:intergenic | 55.0% | |
| TAGGGTGCACTGCATAGGCA+TGG | - | chr8.2:7188892-7188911 | None:intergenic | 55.0% | |
| TGCAGTGCACCCTACACACT+TGG | + | chr8.2:7188898-7188917 | MS.gene051812:CDS | 55.0% | |
| ! | CTGGACACTGTACTGGTCCT+TGG | + | chr8.2:7189274-7189293 | MS.gene051812:CDS | 55.0% |
| GGCTCTTGATTGGGTCACCG+AGG | + | chr8.2:7188961-7188980 | MS.gene051812:CDS | 60.0% | |
| GGTCACCGAGGTTCACGGTA+AGG | + | chr8.2:7188973-7188992 | MS.gene051812:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 7188668 | 7189327 | 7188668 | ID=MS.gene051812 |
| chr8.2 | mRNA | 7188668 | 7189327 | 7188668 | ID=MS.gene051812.t1;Parent=MS.gene051812 |
| chr8.2 | exon | 7188668 | 7189327 | 7188668 | ID=MS.gene051812.t1.exon1;Parent=MS.gene051812.t1 |
| chr8.2 | CDS | 7188668 | 7189327 | 7188668 | ID=cds.MS.gene051812.t1;Parent=MS.gene051812.t1 |
| Gene Sequence |
| Protein sequence |
