Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24916.t1 | XP_003604445.1 | 96.1 | 259 | 10 | 0 | 1 | 259 | 1 | 259 | 1.80E-140 | 509.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24916.t1 | Q6DJF8 | 26.9 | 119 | 85 | 1 | 62 | 178 | 43 | 161 | 1.6e-09 | 65.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24916.t1 | G7JI76 | 96.1 | 259 | 10 | 0 | 1 | 259 | 1 | 259 | 1.3e-140 | 509.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058906 | MS.gene24916 | 0.820672 | 6.06E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24916.t1 | MTR_4g012440 | 96.139 | 259 | 10 | 0 | 1 | 259 | 1 | 259 | 0.0 | 511 |
| MS.gene24916.t1 | MTR_4g012450 | 94.667 | 150 | 8 | 0 | 260 | 409 | 43 | 192 | 6.27e-100 | 296 |
| MS.gene24916.t1 | MTR_2g102080 | 41.096 | 146 | 83 | 2 | 261 | 405 | 11 | 154 | 7.76e-32 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24916.t1 | AT5G01470 | 67.755 | 245 | 71 | 3 | 1 | 244 | 1 | 238 | 4.66e-119 | 346 |
| MS.gene24916.t1 | AT5G01470 | 67.755 | 245 | 71 | 3 | 1 | 244 | 25 | 262 | 1.02e-118 | 346 |
| MS.gene24916.t1 | AT5G01470 | 68.163 | 245 | 66 | 4 | 1 | 244 | 1 | 234 | 1.82e-118 | 345 |
| MS.gene24916.t1 | AT5G01470 | 68.163 | 245 | 66 | 4 | 1 | 244 | 25 | 258 | 3.65e-118 | 345 |
| MS.gene24916.t1 | AT5G01470 | 68.163 | 245 | 64 | 4 | 1 | 244 | 25 | 256 | 4.38e-118 | 345 |
| MS.gene24916.t1 | AT5G01470 | 65.027 | 183 | 53 | 3 | 1 | 182 | 1 | 173 | 3.60e-77 | 237 |
| MS.gene24916.t1 | AT5G01470 | 65.746 | 181 | 51 | 3 | 1 | 180 | 25 | 195 | 4.51e-77 | 238 |
| MS.gene24916.t1 | AT1G52330 | 39.865 | 148 | 86 | 2 | 258 | 404 | 66 | 211 | 1.25e-27 | 109 |
| MS.gene24916.t1 | AT4G13270 | 37.692 | 130 | 78 | 2 | 279 | 407 | 88 | 215 | 9.00e-21 | 90.5 |
| MS.gene24916.t1 | AT1G52330 | 39.024 | 123 | 74 | 1 | 258 | 379 | 66 | 188 | 3.16e-20 | 89.4 |
Find 94 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATAATACCTTGAAAATATT+AGG | 0.209336 | 4.1:-83683095 | None:intergenic |
| TCCCAATCAGGGTCAGAATT+TGG | 0.252944 | 4.1:-83679903 | None:intergenic |
| GGTTGGCACGTGAGGGGTTG+GGG | 0.288499 | 4.1:+83682924 | MS.gene24916:CDS |
| AAAACTATCATGTGAGGTTT+TGG | 0.312823 | 4.1:+83684195 | MS.gene24916:CDS |
| GGGTTGGCACGTGAGGGGTT+GGG | 0.315800 | 4.1:+83682923 | MS.gene24916:CDS |
| TTCCAAATTCTGACCCTGAT+TGG | 0.348490 | 4.1:+83679901 | MS.gene24916:CDS |
| GAAATCGTCTGTATCAGATT+TGG | 0.348510 | 4.1:-83680999 | None:intergenic |
| AAAGTTACAAGCACAGTGAT+AGG | 0.357656 | 4.1:+83681033 | MS.gene24916:CDS |
| CTATGATGATCAAGAAATAA+TGG | 0.365015 | 4.1:+83679260 | MS.gene24916:CDS |
| AATGACACATCAGCACTTAA+TGG | 0.368827 | 4.1:-83682792 | None:intergenic |
| TGGGGTTCAGAACATGTGTT+TGG | 0.368897 | 4.1:+83682942 | MS.gene24916:CDS |
| AAACTTTACATTACCATTCC+AGG | 0.372148 | 4.1:-83672514 | None:intergenic |
| TGCATCAAATGTGCCACATC+TGG | 0.379209 | 4.1:-83682993 | None:intergenic |
| GCAATTATCAGGTCCCAATC+AGG | 0.386063 | 4.1:-83679915 | None:intergenic |
| GGGGTTGGCACGTGAGGGGT+TGG | 0.386737 | 4.1:+83682922 | MS.gene24916:CDS |
| TCCAAATTCTGACCCTGATT+GGG | 0.389989 | 4.1:+83679902 | MS.gene24916:CDS |
| GGTCTACAAGTAAAACATAT+TGG | 0.400170 | 4.1:+83681391 | MS.gene24916:CDS |
| AGATGTCACTCGCAATTATC+AGG | 0.404815 | 4.1:-83679926 | None:intergenic |
| CACACTGCCGTTGGAGTTGT+GGG | 0.405445 | 4.1:+83683044 | MS.gene24916:CDS |
| ATGAGCTGGAGACGTAGAAT+TGG | 0.406397 | 4.1:+83681325 | MS.gene24916:CDS |
| ATAATACCTTGAAAATATTA+GGG | 0.407851 | 4.1:-83683094 | None:intergenic |
| TATCAGTGGCACAGGTGCTT+TGG | 0.413057 | 4.1:+83679191 | MS.gene24916:intron |
| TCTGCAAATGCAAAAGTTCC+TGG | 0.414507 | 4.1:-83678674 | None:intergenic |
| GTGTTTGGTGAACTTGAGTA+TGG | 0.416180 | 4.1:+83682957 | MS.gene24916:CDS |
| GTTGGAGTTGTGGGAAATCT+TGG | 0.428858 | 4.1:+83683053 | MS.gene24916:CDS |
| GGACGGCGTACCATTGAGTT+GGG | 0.432784 | 4.1:+83678728 | MS.gene24916:CDS |
| GTTGATGAAAGGAAAATTCT+CGG | 0.437679 | 4.1:-83678631 | None:intergenic |
| ATTAAACCCAGAAATTGAAA+TGG | 0.437917 | 4.1:+83682728 | MS.gene24916:intron |
| CAGGAGGTGTAGCGTTGCCT+TGG | 0.441681 | 4.1:+83681290 | MS.gene24916:intron |
| ACATCAACATTGTGTTGAAA+AGG | 0.450143 | 4.1:+83684243 | MS.gene24916:CDS |
| CTGATGCTTCCTTTCAACAT+AGG | 0.466349 | 4.1:-83672488 | None:intergenic |
| GTCATCGTCATCATCTTCTT+CGG | 0.472522 | 4.1:-83672360 | None:intergenic |
| AGGGCTACCGTTTCTCCGAC+TGG | 0.472960 | 4.1:+83681053 | MS.gene24916:CDS |
| AACTGAATGCTAATTTGCTC+TGG | 0.484021 | 4.1:+83678651 | MS.gene24916:CDS |
| CAGCACTTAATGGTGGTAAC+GGG | 0.484037 | 4.1:-83682782 | None:intergenic |
| TTCAATGGTTGTGAGAAAGC+TGG | 0.485161 | 4.1:+83681370 | MS.gene24916:CDS |
| AAACGAGGGGTTGGCACGTG+AGG | 0.485667 | 4.1:+83682916 | MS.gene24916:CDS |
| TCACACTGCCGTTGGAGTTG+TGG | 0.488121 | 4.1:+83683043 | MS.gene24916:CDS |
| CTCCAGCTCATCAGAAAAGC+TGG | 0.498474 | 4.1:-83681313 | None:intergenic |
| GGCACATTTGATGCAGGATC+TGG | 0.501360 | 4.1:+83683001 | MS.gene24916:CDS |
| AATGCTAATTTGCTCTGGCC+AGG | 0.508446 | 4.1:+83678656 | MS.gene24916:CDS |
| ATCATTCCCAGTCGGAGAAA+CGG | 0.511706 | 4.1:-83681060 | None:intergenic |
| GAAAGGAAGCATCAGTTCCC+TGG | 0.514240 | 4.1:+83672496 | MS.gene24916:CDS |
| AAGTATCGTGGGCATAAACT+TGG | 0.516870 | 4.1:+83682885 | MS.gene24916:CDS |
| AGGACGGCGTACCATTGAGT+TGG | 0.517895 | 4.1:+83678727 | MS.gene24916:CDS |
| AACTTTACATTACCATTCCA+GGG | 0.524904 | 4.1:-83672513 | None:intergenic |
| AACGAGGGGTTGGCACGTGA+GGG | 0.527288 | 4.1:+83682917 | MS.gene24916:CDS |
| CAACACAGATCATGCATTGA+AGG | 0.531892 | 4.1:+83678707 | MS.gene24916:CDS |
| ATTAGCATTCAGTTGATGAA+AGG | 0.532857 | 4.1:-83678642 | None:intergenic |
| AAAATACCTCCCCAACTCAA+TGG | 0.533914 | 4.1:-83678738 | None:intergenic |
| TGCATCCATATCAGTGGCAC+AGG | 0.548431 | 4.1:+83679183 | MS.gene24916:intron |
| CATCATCTTCTTCGGAGAAG+AGG | 0.552785 | 4.1:-83672352 | None:intergenic |
| AGATGTGGCACATTTGATGC+AGG | 0.554904 | 4.1:+83682995 | MS.gene24916:CDS |
| ATCATCTTCTTCGGAGAAGA+GGG | 0.555067 | 4.1:-83672351 | None:intergenic |
| CTCATGAAACCTATGTTGAA+AGG | 0.555200 | 4.1:+83672479 | MS.gene24916:CDS |
| TGGACTTCCTTCTCCAGATG+TGG | 0.562139 | 4.1:+83682980 | MS.gene24916:CDS |
| TCAGCACTTAATGGTGGTAA+CGG | 0.566066 | 4.1:-83682783 | None:intergenic |
| AACACAATGTTGATGTACAA+TGG | 0.566277 | 4.1:-83684236 | None:intergenic |
| GTCATGTGGAAACGAGGGGT+TGG | 0.567758 | 4.1:+83682907 | MS.gene24916:CDS |
| ACGAGGGGTTGGCACGTGAG+GGG | 0.570453 | 4.1:+83682918 | MS.gene24916:CDS |
| CTCATCAGAAAAGCTGGCCA+AGG | 0.574321 | 4.1:-83681307 | None:intergenic |
| AAGTTACAAGCACAGTGATA+GGG | 0.575432 | 4.1:+83681034 | MS.gene24916:CDS |
| GAAAATATTAGGGAAGTGAA+AGG | 0.575571 | 4.1:-83683084 | None:intergenic |
| AATGTTGATGTACAATGGTA+TGG | 0.578412 | 4.1:-83684231 | None:intergenic |
| GGGCTACCGTTTCTCCGACT+GGG | 0.578578 | 4.1:+83681054 | MS.gene24916:CDS |
| CAATTATCAGGTCCCAATCA+GGG | 0.589733 | 4.1:-83679914 | None:intergenic |
| CTGGAGACGTAGAATTGGAA+AGG | 0.594042 | 4.1:+83681330 | MS.gene24916:CDS |
| AACTTGGTCATGTGGAAACG+AGG | 0.594284 | 4.1:+83682901 | MS.gene24916:CDS |
| AAATGTGCCACATCTGGAGA+AGG | 0.599916 | 4.1:-83682987 | None:intergenic |
| TTTGGTGAACTTGAGTATGG+TGG | 0.601355 | 4.1:+83682960 | MS.gene24916:CDS |
| ATGTGGGCATCAAGTATCGT+GGG | 0.601481 | 4.1:+83682874 | MS.gene24916:CDS |
| TGCAGGATCTGGCTAAGAAG+AGG | 0.601918 | 4.1:+83683012 | MS.gene24916:CDS |
| GAAGCATCAGTTCCCTGGAA+TGG | 0.605315 | 4.1:+83672501 | MS.gene24916:CDS |
| GGAGCTAGCTGAAGTTGATG+TGG | 0.609943 | 4.1:+83682857 | MS.gene24916:CDS |
| GCAGGATCTGGCTAAGAAGA+GGG | 0.611571 | 4.1:+83683013 | MS.gene24916:CDS |
| TGGGCATAAACTTGGTCATG+TGG | 0.622370 | 4.1:+83682893 | MS.gene24916:CDS |
| AACCGTTAAAATTCACAACA+AGG | 0.623265 | 4.1:+83682821 | MS.gene24916:CDS |
| AAGATTTCCCACAACTCCAA+CGG | 0.629032 | 4.1:-83683051 | None:intergenic |
| ACTTGGTCATGTGGAAACGA+GGG | 0.630428 | 4.1:+83682902 | MS.gene24916:CDS |
| GATGTGGGCATCAAGTATCG+TGG | 0.631779 | 4.1:+83682873 | MS.gene24916:CDS |
| GGCGTACCATTGAGTTGGGG+AGG | 0.637262 | 4.1:+83678732 | MS.gene24916:CDS |
| TGGGACTGATGCAATATACA+CGG | 0.643222 | 4.1:-83681417 | None:intergenic |
| GACGATGACATTCATACCAA+GGG | 0.643867 | 4.1:+83672374 | MS.gene24916:CDS |
| ACAGATCATGCATTGAAGGA+CGG | 0.646997 | 4.1:+83678711 | MS.gene24916:CDS |
| ATAGGTAAAACTATCATGTG+AGG | 0.648016 | 4.1:+83684189 | MS.gene24916:intron |
| ACTGGGAATGATGAGACTCA+TGG | 0.651468 | 4.1:+83681071 | MS.gene24916:CDS |
| GGGACTGATGCAATATACAC+GGG | 0.657187 | 4.1:-83681416 | None:intergenic |
| TGACGATGACATTCATACCA+AGG | 0.664322 | 4.1:+83672373 | MS.gene24916:CDS |
| TGAGTCTCATCATTCCCAGT+CGG | 0.667902 | 4.1:-83681068 | None:intergenic |
| GAGCTAGCTGAAGTTGATGT+GGG | 0.670977 | 4.1:+83682858 | MS.gene24916:CDS |
| GACGGCGTACCATTGAGTTG+GGG | 0.674436 | 4.1:+83678729 | MS.gene24916:CDS |
| GACACATCAGCACTTAATGG+TGG | 0.680325 | 4.1:-83682789 | None:intergenic |
| CTTGGTCATGTGGAAACGAG+GGG | 0.682725 | 4.1:+83682903 | MS.gene24916:CDS |
| GAGAACATAGCTCACAACTG+TGG | 0.711730 | 4.1:+83679282 | MS.gene24916:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 83672323 | 83684746 | 83672323 | ID=MS.gene24916 |
| chr4.1 | mRNA | 83672323 | 83684746 | 83672323 | ID=MS.gene24916.t1;Parent=MS.gene24916 |
| chr4.1 | exon | 83672323 | 83672395 | 83672323 | ID=MS.gene24916.t1.exon1;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83672323 | 83672395 | 83672323 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| chr4.1 | exon | 83672461 | 83672522 | 83672461 | ID=MS.gene24916.t1.exon2;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83672461 | 83672522 | 83672461 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| chr4.1 | exon | 83678620 | 83678753 | 83678620 | ID=MS.gene24916.t1.exon3;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83678620 | 83678753 | 83678620 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| chr4.1 | exon | 83679197 | 83679339 | 83679197 | ID=MS.gene24916.t1.exon4;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83679197 | 83679339 | 83679197 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| chr4.1 | exon | 83679883 | 83679954 | 83679883 | ID=MS.gene24916.t1.exon5;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83679883 | 83679954 | 83679883 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| chr4.1 | exon | 83680985 | 83681092 | 83680985 | ID=MS.gene24916.t1.exon6;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83680985 | 83681092 | 83680985 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| chr4.1 | exon | 83681293 | 83681478 | 83681293 | ID=MS.gene24916.t1.exon7;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83681293 | 83681478 | 83681293 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| chr4.1 | exon | 83682739 | 83683109 | 83682739 | ID=MS.gene24916.t1.exon8;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83682739 | 83683109 | 83682739 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| chr4.1 | exon | 83684193 | 83684264 | 83684193 | ID=MS.gene24916.t1.exon9;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83684193 | 83684264 | 83684193 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| chr4.1 | exon | 83684732 | 83684746 | 83684732 | ID=MS.gene24916.t1.exon10;Parent=MS.gene24916.t1 |
| chr4.1 | CDS | 83684732 | 83684746 | 83684732 | ID=cds.MS.gene24916.t1;Parent=MS.gene24916.t1 |
| Gene Sequence |
| Protein sequence |
