Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24970.t1 | KEH28826.1 | 90.7 | 108 | 10 | 0 | 1 | 108 | 92 | 199 | 3.80E-50 | 207.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24970.t1 | Q9C523 | 71.3 | 108 | 31 | 0 | 1 | 108 | 78 | 185 | 2.3e-40 | 166.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24970.t1 | A0A072UHF1 | 90.7 | 108 | 10 | 0 | 1 | 108 | 92 | 199 | 2.7e-50 | 207.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051265 | MS.gene24970 | 0.823727 | 1.19E-53 | -1.69E-46 |
| MS.gene051719 | MS.gene24970 | 0.800986 | 1.11E-48 | -1.69E-46 |
| MS.gene055356 | MS.gene24970 | 0.81599 | 6.96E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24970.t1 | MTR_4g013330 | 90.741 | 108 | 10 | 0 | 1 | 108 | 92 | 199 | 1.81e-71 | 211 |
| MS.gene24970.t1 | MTR_4g013345 | 87.963 | 108 | 13 | 0 | 1 | 108 | 89 | 196 | 1.14e-70 | 209 |
| MS.gene24970.t1 | MTR_4g013350 | 84.259 | 108 | 17 | 0 | 1 | 108 | 90 | 197 | 7.36e-68 | 202 |
| MS.gene24970.t1 | MTR_4g013320 | 79.630 | 108 | 22 | 0 | 1 | 108 | 92 | 199 | 3.89e-63 | 190 |
| MS.gene24970.t1 | MTR_4g013335 | 75.000 | 108 | 27 | 0 | 1 | 108 | 89 | 196 | 1.70e-59 | 181 |
| MS.gene24970.t1 | MTR_4g013325 | 75.000 | 108 | 27 | 0 | 1 | 108 | 92 | 199 | 6.94e-59 | 179 |
| MS.gene24970.t1 | MTR_7g070390 | 74.074 | 108 | 28 | 0 | 1 | 108 | 85 | 192 | 1.45e-57 | 176 |
| MS.gene24970.t1 | MTR_4g013310 | 69.444 | 108 | 31 | 1 | 1 | 108 | 92 | 197 | 1.36e-50 | 158 |
| MS.gene24970.t1 | MTR_4g013385 | 65.741 | 108 | 37 | 0 | 1 | 108 | 83 | 190 | 5.13e-49 | 154 |
| MS.gene24970.t1 | MTR_0433s0040 | 65.741 | 108 | 37 | 0 | 1 | 108 | 83 | 190 | 5.13e-49 | 154 |
| MS.gene24970.t1 | MTR_1g054525 | 66.667 | 108 | 34 | 1 | 1 | 108 | 87 | 192 | 2.81e-48 | 152 |
| MS.gene24970.t1 | MTR_4g013770 | 63.889 | 108 | 39 | 0 | 1 | 108 | 84 | 191 | 4.96e-47 | 149 |
| MS.gene24970.t1 | MTR_4g073950 | 60.185 | 108 | 43 | 0 | 1 | 108 | 85 | 192 | 1.58e-46 | 148 |
| MS.gene24970.t1 | MTR_7g093870 | 57.658 | 111 | 44 | 1 | 1 | 108 | 83 | 193 | 2.50e-42 | 138 |
| MS.gene24970.t1 | MTR_7g093820 | 60.185 | 108 | 42 | 1 | 2 | 108 | 87 | 194 | 3.75e-42 | 137 |
| MS.gene24970.t1 | MTR_7g093850 | 60.185 | 108 | 42 | 1 | 2 | 108 | 87 | 194 | 3.88e-42 | 137 |
| MS.gene24970.t1 | MTR_7g093790 | 57.407 | 108 | 45 | 1 | 2 | 108 | 84 | 191 | 6.55e-42 | 136 |
| MS.gene24970.t1 | MTR_4g013355 | 60.185 | 108 | 33 | 1 | 1 | 108 | 82 | 179 | 7.37e-41 | 133 |
| MS.gene24970.t1 | MTR_7g093830 | 58.333 | 108 | 44 | 1 | 2 | 108 | 86 | 193 | 7.37e-41 | 134 |
| MS.gene24970.t1 | MTR_1g056370 | 53.704 | 108 | 49 | 1 | 1 | 107 | 78 | 185 | 4.01e-37 | 124 |
| MS.gene24970.t1 | MTR_3g105640 | 49.074 | 108 | 54 | 1 | 2 | 108 | 81 | 188 | 6.65e-33 | 113 |
| MS.gene24970.t1 | MTR_4g078885 | 46.296 | 108 | 58 | 0 | 1 | 108 | 78 | 185 | 1.75e-31 | 109 |
| MS.gene24970.t1 | MTR_3g034030 | 46.296 | 108 | 58 | 0 | 1 | 108 | 78 | 185 | 1.17e-30 | 107 |
| MS.gene24970.t1 | MTR_5g096120 | 44.444 | 108 | 59 | 1 | 2 | 108 | 82 | 189 | 2.02e-29 | 104 |
| MS.gene24970.t1 | MTR_3g105630 | 44.444 | 108 | 59 | 1 | 2 | 108 | 82 | 189 | 6.57e-29 | 103 |
| MS.gene24970.t1 | MTR_4g122110 | 37.963 | 108 | 67 | 0 | 1 | 108 | 73 | 180 | 8.07e-21 | 82.4 |
| MS.gene24970.t1 | MTR_4g122130 | 37.037 | 108 | 68 | 0 | 1 | 108 | 73 | 180 | 2.45e-19 | 78.6 |
| MS.gene24970.t1 | MTR_4g049570 | 32.323 | 99 | 67 | 0 | 1 | 99 | 71 | 169 | 4.37e-13 | 62.0 |
| MS.gene24970.t1 | MTR_4g049550 | 34.940 | 83 | 54 | 0 | 1 | 83 | 71 | 153 | 5.43e-13 | 62.0 |
| MS.gene24970.t1 | MTR_8g099115 | 35.632 | 87 | 55 | 1 | 1 | 87 | 83 | 168 | 2.52e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24970.t1 | AT1G58170 | 71.296 | 108 | 31 | 0 | 1 | 108 | 78 | 185 | 3.53e-55 | 170 |
| MS.gene24970.t1 | AT1G55210 | 65.741 | 108 | 37 | 0 | 1 | 108 | 80 | 187 | 3.92e-49 | 154 |
| MS.gene24970.t1 | AT1G55210 | 65.741 | 108 | 37 | 0 | 1 | 108 | 80 | 187 | 3.92e-49 | 154 |
| MS.gene24970.t1 | AT3G13660 | 63.889 | 108 | 39 | 0 | 1 | 108 | 18 | 125 | 6.46e-47 | 147 |
| MS.gene24970.t1 | AT5G49040 | 62.037 | 108 | 41 | 0 | 1 | 108 | 84 | 191 | 3.36e-45 | 144 |
| MS.gene24970.t1 | AT3G13650 | 61.111 | 108 | 42 | 0 | 1 | 108 | 79 | 186 | 8.45e-43 | 138 |
| MS.gene24970.t1 | AT3G13662 | 67.368 | 95 | 31 | 0 | 1 | 95 | 78 | 172 | 3.04e-41 | 134 |
| MS.gene24970.t1 | AT2G21100 | 53.271 | 107 | 50 | 0 | 1 | 107 | 81 | 187 | 5.95e-40 | 131 |
| MS.gene24970.t1 | AT2G21100 | 53.271 | 107 | 50 | 0 | 1 | 107 | 81 | 187 | 5.95e-40 | 131 |
| MS.gene24970.t1 | AT1G65870 | 51.402 | 107 | 52 | 0 | 1 | 107 | 83 | 189 | 1.54e-37 | 125 |
| MS.gene24970.t1 | AT3G58090 | 50.467 | 107 | 53 | 0 | 1 | 107 | 165 | 271 | 2.50e-36 | 124 |
| MS.gene24970.t1 | AT5G49030 | 63.830 | 94 | 34 | 0 | 1 | 94 | 84 | 177 | 6.04e-35 | 126 |
| MS.gene24970.t1 | AT5G42500 | 53.271 | 107 | 48 | 1 | 1 | 107 | 81 | 185 | 1.42e-34 | 117 |
| MS.gene24970.t1 | AT5G42510 | 50.467 | 107 | 51 | 1 | 1 | 107 | 78 | 182 | 1.74e-33 | 114 |
| MS.gene24970.t1 | AT1G22900 | 51.402 | 107 | 50 | 1 | 1 | 107 | 89 | 193 | 1.76e-33 | 115 |
| MS.gene24970.t1 | AT2G21110 | 45.794 | 107 | 58 | 0 | 2 | 108 | 80 | 186 | 1.79e-30 | 107 |
| MS.gene24970.t1 | AT4G38700 | 42.857 | 112 | 60 | 2 | 1 | 108 | 79 | 190 | 3.81e-28 | 101 |
Find 27 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGACAGCCCTTTGACTTT+AGG | 0.270088 | 4.1:+82379245 | MS.gene24970:CDS |
| CATTGGGTTGATCTCAAATC+TGG | 0.338134 | 4.1:+82379503 | MS.gene24970:CDS |
| GGTAGTACTATTACTATCTT+GGG | 0.346488 | 4.1:+82379386 | MS.gene24970:CDS |
| TTCATTGAAGGAAAGTATAA+TGG | 0.359474 | 4.1:+82379365 | MS.gene24970:CDS |
| TGGTAGTACTATTACTATCT+TGG | 0.363892 | 4.1:+82379385 | MS.gene24970:CDS |
| TCTGCATCACAGGAAGAAAT+AGG | 0.369275 | 4.1:+82379317 | MS.gene24970:CDS |
| CAATTGAGTTCCAAACTTGT+TGG | 0.370040 | 4.1:+82379272 | MS.gene24970:CDS |
| ATGCCTGTTGTTGGTGGTAG+TGG | 0.375988 | 4.1:+82379440 | MS.gene24970:CDS |
| ATGCTCAAGCTAGTACTCAT+TGG | 0.446414 | 4.1:+82379486 | MS.gene24970:CDS |
| ATCATGCTCTTGATAAGGTT+AGG | 0.460618 | 4.1:+82379414 | MS.gene24970:CDS |
| TGCTCAAGCTAGTACTCATT+GGG | 0.464742 | 4.1:+82379487 | MS.gene24970:CDS |
| TTTCTATGCATCTGCATCAC+AGG | 0.485104 | 4.1:+82379307 | MS.gene24970:CDS |
| ATTGGGTTGATCTCAAATCT+GGG | 0.503082 | 4.1:+82379504 | MS.gene24970:CDS |
| GTTAGGGAGATGCCTGTTGT+TGG | 0.505285 | 4.1:+82379431 | MS.gene24970:CDS |
| AGTCCACTACCACCAACAAC+AGG | 0.512977 | 4.1:-82379443 | None:intergenic |
| TCATGCTCTTGATAAGGTTA+GGG | 0.514769 | 4.1:+82379415 | MS.gene24970:CDS |
| AAACTTGTTGGAAAAGCTCA+AGG | 0.519817 | 4.1:+82379284 | MS.gene24970:CDS |
| AGGGAGATGCCTGTTGTTGG+TGG | 0.521357 | 4.1:+82379434 | MS.gene24970:CDS |
| GAGGAATCATGCTCTTGATA+AGG | 0.521522 | 4.1:+82379409 | MS.gene24970:CDS |
| AATTGCGGTCCTAAAGTCAA+AGG | 0.528813 | 4.1:-82379254 | None:intergenic |
| ATTGCGGTCCTAAAGTCAAA+GGG | 0.563989 | 4.1:-82379253 | None:intergenic |
| AAATCTGGGGATGCATGTGT+TGG | 0.566058 | 4.1:+82379518 | MS.gene24970:CDS |
| AATCTGGGGATGCATGTGTT+GGG | 0.577672 | 4.1:+82379519 | MS.gene24970:CDS |
| ACAAGTTTGGAACTCAATTG+CGG | 0.641411 | 4.1:-82379269 | None:intergenic |
| TTGGGTTGATCTCAAATCTG+GGG | 0.653245 | 4.1:+82379505 | MS.gene24970:CDS |
| GTACTATTACTATCTTGGGG+AGG | 0.673978 | 4.1:+82379390 | MS.gene24970:CDS |
| GTAGTACTATTACTATCTTG+GGG | 0.684734 | 4.1:+82379387 | MS.gene24970:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAATTTTGCTTTCATTGA+AGG | + | chr4.1:82379353-82379372 | MS.gene24970:CDS | 25.0% |
| !! | TTCATTGAAGGAAAGTATAA+TGG | + | chr4.1:82379365-82379384 | MS.gene24970:CDS | 25.0% |
| GGTAGTACTATTACTATCTT+GGG | + | chr4.1:82379386-82379405 | MS.gene24970:CDS | 30.0% | |
| GTAGTACTATTACTATCTTG+GGG | + | chr4.1:82379387-82379406 | MS.gene24970:CDS | 30.0% | |
| TGGTAGTACTATTACTATCT+TGG | + | chr4.1:82379385-82379404 | MS.gene24970:CDS | 30.0% | |
| ATCATGCTCTTGATAAGGTT+AGG | + | chr4.1:82379414-82379433 | MS.gene24970:CDS | 35.0% | |
| CAATTGAGTTCCAAACTTGT+TGG | + | chr4.1:82379272-82379291 | MS.gene24970:CDS | 35.0% | |
| TCATGCTCTTGATAAGGTTA+GGG | + | chr4.1:82379415-82379434 | MS.gene24970:CDS | 35.0% | |
| ! | ACAAGTTTGGAACTCAATTG+CGG | - | chr4.1:82379272-82379291 | None:intergenic | 35.0% |
| ! | TTGAGCTTTTCCAACAAGTT+TGG | - | chr4.1:82379285-82379304 | None:intergenic | 35.0% |
| !! | AAACTTGTTGGAAAAGCTCA+AGG | + | chr4.1:82379284-82379303 | MS.gene24970:CDS | 35.0% |
| !! | ATTGGGTTGATCTCAAATCT+GGG | + | chr4.1:82379504-82379523 | MS.gene24970:CDS | 35.0% |
| !!! | GACTTTTCAGATTTGCTAGA+GGG | + | chr4.1:82379462-82379481 | MS.gene24970:CDS | 35.0% |
| AATTGCGGTCCTAAAGTCAA+AGG | - | chr4.1:82379257-82379276 | None:intergenic | 40.0% | |
| ATGCTCAAGCTAGTACTCAT+TGG | + | chr4.1:82379486-82379505 | MS.gene24970:CDS | 40.0% | |
| ATTGCGGTCCTAAAGTCAAA+GGG | - | chr4.1:82379256-82379275 | None:intergenic | 40.0% | |
| GAGGAATCATGCTCTTGATA+AGG | + | chr4.1:82379409-82379428 | MS.gene24970:CDS | 40.0% | |
| GTACTATTACTATCTTGGGG+AGG | + | chr4.1:82379390-82379409 | MS.gene24970:CDS | 40.0% | |
| TCTGCATCACAGGAAGAAAT+AGG | + | chr4.1:82379317-82379336 | MS.gene24970:CDS | 40.0% | |
| TGCTCAAGCTAGTACTCATT+GGG | + | chr4.1:82379487-82379506 | MS.gene24970:CDS | 40.0% | |
| TTTCTATGCATCTGCATCAC+AGG | + | chr4.1:82379307-82379326 | MS.gene24970:CDS | 40.0% | |
| !! | CATTGGGTTGATCTCAAATC+TGG | + | chr4.1:82379503-82379522 | MS.gene24970:CDS | 40.0% |
| !! | TTGGGTTGATCTCAAATCTG+GGG | + | chr4.1:82379505-82379524 | MS.gene24970:CDS | 40.0% |
| !!! | GGACTTTTCAGATTTGCTAG+AGG | + | chr4.1:82379461-82379480 | MS.gene24970:CDS | 40.0% |
| AAATCTGGGGATGCATGTGT+TGG | + | chr4.1:82379518-82379537 | MS.gene24970:CDS | 45.0% | |
| AATCTGGGGATGCATGTGTT+GGG | + | chr4.1:82379519-82379538 | MS.gene24970:CDS | 45.0% | |
| ! | ATGGACAGCCCTTTGACTTT+AGG | + | chr4.1:82379245-82379264 | MS.gene24970:CDS | 45.0% |
| AGTCCACTACCACCAACAAC+AGG | - | chr4.1:82379446-82379465 | None:intergenic | 50.0% | |
| GTTAGGGAGATGCCTGTTGT+TGG | + | chr4.1:82379431-82379450 | MS.gene24970:CDS | 50.0% | |
| !! | ATGCCTGTTGTTGGTGGTAG+TGG | + | chr4.1:82379440-82379459 | MS.gene24970:CDS | 50.0% |
| AGGGAGATGCCTGTTGTTGG+TGG | + | chr4.1:82379434-82379453 | MS.gene24970:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 82379242 | 82379568 | 82379242 | ID=MS.gene24970 |
| chr4.1 | mRNA | 82379242 | 82379568 | 82379242 | ID=MS.gene24970.t1;Parent=MS.gene24970 |
| chr4.1 | exon | 82379242 | 82379568 | 82379242 | ID=MS.gene24970.t1.exon1;Parent=MS.gene24970.t1 |
| chr4.1 | CDS | 82379242 | 82379568 | 82379242 | ID=cds.MS.gene24970.t1;Parent=MS.gene24970.t1 |
| Gene Sequence |
| Protein sequence |
