AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25004

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25004.t1 MTR_8g023860 79.153 307 25 4 1 269 1 306 2.15e-157 445
MS.gene25004.t1 MTR_8g023840 58.934 319 78 6 1 269 1 316 1.68e-108 320
MS.gene25004.t1 MTR_8g023900 57.143 280 78 4 1 240 184 461 1.74e-103 309
MS.gene25004.t1 MTR_8g023900 52.973 185 54 3 1 152 1 185 4.08e-56 187
MS.gene25004.t1 MTR_8g023930 59.480 269 68 4 10 238 22 289 3.63e-102 306
MS.gene25004.t1 MTR_8g023880 38.043 276 101 10 12 238 7 261 2.45e-41 144
MS.gene25004.t1 MTR_5g069030 35.036 274 118 11 8 237 21 278 1.97e-30 120
MS.gene25004.t1 MTR_3g093050 41.718 163 57 5 8 134 18 178 2.09e-29 117
MS.gene25004.t1 MTR_3g093050 41.718 163 57 5 8 134 18 178 2.75e-29 116
MS.gene25004.t1 MTR_3g093040 40.437 183 62 7 1 146 100 272 4.22e-29 112
MS.gene25004.t1 MTR_6g477900 38.690 168 63 6 12 142 6 170 4.72e-27 105
MS.gene25004.t1 MTR_3g064580 37.363 182 73 5 12 155 6 184 1.87e-26 108
MS.gene25004.t1 MTR_3g109340 39.735 151 57 4 12 129 22 171 8.91e-26 104
MS.gene25004.t1 MTR_3g109340 39.474 152 57 5 12 129 22 172 1.32e-24 101
MS.gene25004.t1 MTR_5g014300 36.145 166 66 5 12 138 6 170 4.47e-24 99.8
MS.gene25004.t1 MTR_4g098630 37.433 187 76 7 12 160 6 189 5.19e-24 99.4
MS.gene25004.t1 MTR_4g036030 38.854 157 57 5 8 128 3 156 5.58e-24 99.8
MS.gene25004.t1 MTR_7g005280 35.119 168 77 5 11 148 14 179 7.50e-24 97.4
MS.gene25004.t1 MTR_5g012080 38.994 159 54 6 9 128 4 158 1.97e-23 98.2
MS.gene25004.t1 MTR_8g099750 36.774 155 59 5 9 125 11 164 2.03e-23 95.5
MS.gene25004.t1 MTR_2g064090 36.774 155 57 5 12 128 6 157 3.33e-23 97.4
MS.gene25004.t1 MTR_4g101680 36.095 169 69 5 8 140 3 168 4.05e-23 97.4
MS.gene25004.t1 MTR_1g069805 35.570 149 66 4 12 130 10 158 8.07e-23 95.1
MS.gene25004.t1 MTR_6g084430 37.423 163 64 7 10 137 3 162 8.72e-23 95.9
MS.gene25004.t1 MTR_3g070030 40.994 161 51 8 13 132 20 177 8.94e-23 96.3
MS.gene25004.t1 MTR_2g078700 41.085 129 45 3 11 109 22 149 1.18e-22 96.3
MS.gene25004.t1 MTR_2g064470 36.585 164 69 6 12 142 11 172 1.28e-22 95.1
MS.gene25004.t1 MTR_3g435150 40.000 155 53 7 12 129 16 167 3.02e-22 94.0
MS.gene25004.t1 MTR_1g096430 34.884 172 73 5 12 147 7 175 3.92e-22 94.4
MS.gene25004.t1 MTR_5g076850 36.774 155 58 5 12 129 6 157 4.30e-22 95.9
MS.gene25004.t1 MTR_2g062730 28.926 242 118 7 12 208 10 242 7.45e-22 93.6
MS.gene25004.t1 MTR_4g108760 38.065 155 56 7 12 129 17 168 9.55e-22 93.6
MS.gene25004.t1 MTR_7g097090 34.932 146 63 4 12 126 10 154 1.25e-21 92.0
MS.gene25004.t1 MTR_4g035590 39.610 154 51 7 12 128 8 156 2.22e-21 92.4
MS.gene25004.t1 MTR_1g090723 38.158 152 55 5 15 129 11 160 3.47e-21 93.2
MS.gene25004.t1 MTR_1g008740 34.868 152 65 4 12 129 13 164 3.78e-21 91.7
MS.gene25004.t1 MTR_1g008740 34.868 152 65 4 12 129 27 178 3.92e-21 92.0
MS.gene25004.t1 MTR_3g116070 36.000 150 57 3 12 122 23 172 3.99e-21 91.7
MS.gene25004.t1 MTR_1g090723 37.748 151 56 5 15 129 11 159 8.26e-21 92.0
MS.gene25004.t1 MTR_4g081870 37.975 158 52 7 12 128 9 161 8.86e-21 89.4
MS.gene25004.t1 MTR_1g090723 37.748 151 56 5 15 129 11 159 9.43e-21 92.0
MS.gene25004.t1 MTR_2g086880 36.250 160 58 7 2 130 16 162 1.31e-20 91.7
MS.gene25004.t1 MTR_2g080010 32.727 165 64 4 11 129 14 177 1.34e-20 90.5
MS.gene25004.t1 MTR_5g021710 37.255 153 57 5 12 128 8 157 1.39e-20 90.1
MS.gene25004.t1 MTR_8g076110 35.948 153 59 5 12 128 6 155 1.73e-20 89.4
MS.gene25004.t1 MTR_2g086880 36.250 160 58 7 2 130 16 162 2.41e-20 90.9
MS.gene25004.t1 MTR_3g070040 36.875 160 62 6 14 137 10 166 2.52e-20 89.4
MS.gene25004.t1 MTR_4g089135 35.714 140 59 3 11 120 14 152 3.82e-20 89.0
MS.gene25004.t1 MTR_2g093810 35.897 156 60 5 13 129 15 169 1.06e-19 87.4
MS.gene25004.t1 MTR_7g011130 42.857 126 39 5 14 109 21 143 1.51e-19 87.4
MS.gene25004.t1 MTR_7g100990 33.121 157 65 5 11 128 10 165 1.63e-19 87.0
MS.gene25004.t1 MTR_3g096920 35.443 158 60 5 12 131 7 160 3.40e-19 85.5
MS.gene25004.t1 MTR_1g097300 38.065 155 55 7 5 127 13 158 7.23e-19 86.7
MS.gene25004.t1 MTR_5g041940 35.119 168 65 8 12 140 3 165 7.63e-19 84.0
MS.gene25004.t1 MTR_8g467490 32.121 165 67 4 15 135 17 180 8.50e-19 85.5
MS.gene25004.t1 MTR_3g098810 32.927 164 68 5 12 134 14 176 8.51e-19 83.6
MS.gene25004.t1 MTR_6g032770 36.719 128 50 3 12 109 18 144 8.74e-19 85.5
MS.gene25004.t1 MTR_8g094580 33.129 163 58 6 12 131 7 161 1.03e-18 84.0
MS.gene25004.t1 MTR_4g052620 33.533 167 61 5 1 125 1 159 1.22e-18 83.6
MS.gene25004.t1 MTR_4g075980 37.500 160 64 7 15 138 18 177 1.84e-18 83.6
MS.gene25004.t1 MTR_3g088110 33.974 156 61 5 11 128 6 157 2.34e-18 83.2
MS.gene25004.t1 MTR_7g011120 42.857 126 39 5 14 109 10 132 2.86e-18 83.2
MS.gene25004.t1 MTR_7g105170 32.738 168 65 7 9 130 1 166 3.51e-18 82.0
MS.gene25004.t1 MTR_3g096920 39.370 127 43 4 12 108 7 129 3.55e-18 83.2
MS.gene25004.t1 MTR_7g085260 38.346 133 48 5 8 108 14 144 4.50e-18 83.6
MS.gene25004.t1 MTR_0036s0150 35.762 151 59 6 15 129 8 156 5.63e-18 84.0
MS.gene25004.t1 MTR_3g098810 36.923 130 51 4 9 108 11 139 6.47e-18 79.7
MS.gene25004.t1 MTR_5g040420 29.744 195 92 5 9 159 10 203 1.27e-17 82.4
MS.gene25004.t1 MTR_1g090720 34.591 159 54 6 15 128 45 198 1.47e-17 82.4
MS.gene25004.t1 MTR_4g134460 34.868 152 66 5 10 129 52 202 5.74e-17 80.5
MS.gene25004.t1 MTR_8g059170 29.302 215 109 8 12 187 14 224 2.11e-16 78.2
MS.gene25004.t1 MTR_2g086690 32.692 156 61 5 13 125 5 159 1.10e-15 75.1
MS.gene25004.t1 MTR_8g093790 32.903 155 72 6 12 144 10 154 1.53e-15 73.6
MS.gene25004.t1 MTR_4g094302 28.916 166 80 6 12 142 11 173 1.13e-14 71.2
MS.gene25004.t1 MTR_4g094302 35.537 121 51 5 12 108 11 128 6.00e-14 68.6
MS.gene25004.t1 MTR_7g070140 31.667 120 63 3 47 165 27 128 5.20e-13 65.1
MS.gene25004.t1 MTR_3g096400 30.065 153 67 4 12 125 7 158 6.20e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25004.t1 AT4G01550 42.529 174 64 4 14 154 6 176 5.02e-34 128
MS.gene25004.t1 AT4G01520 33.624 229 100 7 13 197 5 225 6.50e-31 117
MS.gene25004.t1 AT4G01540 33.772 228 99 7 14 197 6 225 1.38e-29 115
MS.gene25004.t1 AT4G01540 33.772 228 99 7 14 197 6 225 1.93e-29 115
MS.gene25004.t1 AT4G01540 33.040 227 102 6 14 197 6 225 2.10e-29 115
MS.gene25004.t1 AT4G01540 33.772 228 99 7 14 197 6 225 2.76e-29 115
MS.gene25004.t1 AT3G49530 40.741 162 53 6 8 130 9 166 2.69e-28 112
MS.gene25004.t1 AT3G49530 40.373 161 55 6 8 130 9 166 4.31e-28 112
MS.gene25004.t1 AT5G24590 38.889 180 68 7 8 148 9 185 6.96e-27 108
MS.gene25004.t1 AT4G35580 38.272 162 60 5 8 132 5 163 1.18e-26 108
MS.gene25004.t1 AT4G35580 38.272 162 60 5 8 132 5 163 1.24e-26 108
MS.gene25004.t1 AT4G35580 38.272 162 60 5 8 132 5 163 1.51e-26 108
MS.gene25004.t1 AT1G33060 40.506 158 54 5 8 128 20 174 1.69e-26 108
MS.gene25004.t1 AT1G33060 40.506 158 54 5 8 128 20 174 1.70e-26 108
MS.gene25004.t1 AT3G17730 37.791 172 63 7 12 142 6 174 5.26e-26 103
MS.gene25004.t1 AT4G17980 32.489 237 111 8 12 202 6 239 4.05e-25 100
MS.gene25004.t1 AT3G10490 39.474 152 51 5 15 128 30 178 5.57e-25 100
MS.gene25004.t1 AT4G17980 32.489 237 111 8 12 202 6 239 6.93e-25 101
MS.gene25004.t1 AT2G24430 37.908 153 60 5 12 130 16 167 1.03e-24 101
MS.gene25004.t1 AT2G24430 37.908 153 60 5 12 130 16 167 1.03e-24 101
MS.gene25004.t1 AT3G10490 39.474 152 51 5 15 128 30 178 1.32e-24 102
MS.gene25004.t1 AT3G10480 38.816 152 52 5 15 128 30 178 2.16e-24 102
MS.gene25004.t1 AT5G17260 38.710 155 54 5 12 128 6 157 2.21e-24 102
MS.gene25004.t1 AT3G10480 39.073 151 52 5 15 128 30 177 2.32e-24 102
MS.gene25004.t1 AT5G46590 40.625 160 55 6 12 133 6 163 5.68e-24 98.6
MS.gene25004.t1 AT1G12260 37.580 157 59 5 8 128 3 156 1.42e-23 98.6
MS.gene25004.t1 AT1G62700 37.580 157 59 5 8 128 3 156 1.50e-23 99.0
MS.gene25004.t1 AT1G62700 37.580 157 59 5 8 128 3 156 1.50e-23 99.0
MS.gene25004.t1 AT1G12260 37.580 157 59 5 8 128 3 156 1.96e-23 98.6
MS.gene25004.t1 AT3G15170 38.710 155 57 5 12 129 20 173 2.75e-23 97.1
MS.gene25004.t1 AT1G65910 38.710 155 55 5 12 129 6 157 3.45e-23 99.4
MS.gene25004.t1 AT5G62380 37.580 157 59 5 8 128 3 156 3.95e-23 97.4
MS.gene25004.t1 AT5G62380 37.580 157 59 5 8 128 3 156 3.95e-23 97.4
MS.gene25004.t1 AT5G61430 39.355 155 54 7 12 129 16 167 4.23e-23 97.1
MS.gene25004.t1 AT1G69490 37.179 156 56 6 12 128 9 161 1.38e-22 94.4
MS.gene25004.t1 AT3G03200 38.065 155 55 5 12 128 6 157 2.55e-22 96.3
MS.gene25004.t1 AT5G53950 41.085 129 45 3 10 108 15 142 3.30e-22 95.1
MS.gene25004.t1 AT1G33280 39.474 152 54 5 12 128 8 156 3.43e-22 94.0
MS.gene25004.t1 AT5G39610 37.662 154 56 6 12 128 20 170 3.48e-22 93.6
MS.gene25004.t1 AT5G13180 34.899 149 65 4 11 128 13 160 1.11e-21 91.7
MS.gene25004.t1 AT1G76420 35.714 154 58 5 12 128 22 171 1.16e-21 93.2
MS.gene25004.t1 AT3G18400 32.500 200 95 7 10 172 3 199 1.33e-21 92.4
MS.gene25004.t1 AT5G66300 36.364 154 58 5 12 128 12 162 2.10e-21 91.7
MS.gene25004.t1 AT3G04060 35.714 154 60 6 12 128 20 171 2.69e-21 92.0
MS.gene25004.t1 AT1G54330 39.073 151 55 5 12 128 6 153 5.61e-21 90.5
MS.gene25004.t1 AT5G46590 41.538 130 42 4 12 109 6 133 5.81e-21 90.1
MS.gene25004.t1 AT2G18060 35.976 164 66 5 12 139 9 169 6.74e-21 91.3
MS.gene25004.t1 AT2G18060 35.976 164 66 5 12 139 9 169 6.74e-21 91.3
MS.gene25004.t1 AT1G71930 35.948 153 59 5 12 128 9 158 1.16e-20 90.1
MS.gene25004.t1 AT1G71930 35.948 153 59 5 12 128 9 158 1.16e-20 90.1
MS.gene25004.t1 AT1G79580 38.158 152 57 5 12 128 17 166 1.33e-20 90.5
MS.gene25004.t1 AT1G79580 38.158 152 57 5 12 128 17 166 1.33e-20 90.5
MS.gene25004.t1 AT1G79580 38.158 152 57 5 12 128 17 166 1.33e-20 90.5
MS.gene25004.t1 AT1G79580 38.158 152 57 5 12 128 17 166 1.33e-20 90.5
MS.gene25004.t1 AT1G79580 38.158 152 57 5 12 128 17 166 1.33e-20 90.5
MS.gene25004.t1 AT1G32510 33.184 223 92 10 12 183 6 222 1.40e-20 89.4
MS.gene25004.t1 AT5G18270 29.487 234 115 7 12 206 21 243 1.70e-20 89.7
MS.gene25004.t1 AT5G18270 29.487 234 115 7 12 206 21 243 1.82e-20 89.7
MS.gene25004.t1 AT3G61910 31.280 211 78 8 11 167 10 207 2.46e-20 89.4
MS.gene25004.t1 AT1G56010 34.839 155 61 4 12 128 19 171 2.69e-20 89.0
MS.gene25004.t1 AT2G33480 36.486 148 61 5 11 128 14 158 2.96e-20 88.2
MS.gene25004.t1 AT2G33480 36.000 150 61 5 11 128 14 160 4.72e-20 87.4
MS.gene25004.t1 AT1G02230 37.255 153 60 5 13 131 4 154 5.39e-20 88.6
MS.gene25004.t1 AT4G10350 36.686 169 65 6 12 141 9 174 6.36e-20 88.2
MS.gene25004.t1 AT1G01720 35.220 159 60 6 9 128 4 158 8.20e-20 87.4
MS.gene25004.t1 AT3G10500 34.742 213 91 12 15 188 12 215 1.10e-19 89.0
MS.gene25004.t1 AT3G04070 34.356 163 61 5 12 129 10 171 1.22e-19 87.4
MS.gene25004.t1 AT5G22380 34.810 158 60 5 10 125 3 159 1.24e-19 85.9
MS.gene25004.t1 AT1G26870 30.802 237 89 8 15 181 19 250 1.29e-19 88.2
MS.gene25004.t1 AT5G39820 40.441 136 50 3 10 115 18 152 1.32e-19 87.4
MS.gene25004.t1 AT4G36160 35.948 153 59 5 12 128 10 159 1.50e-19 87.8
MS.gene25004.t1 AT4G36160 35.948 153 59 5 12 128 10 159 1.50e-19 87.8
MS.gene25004.t1 AT5G04410 36.842 152 56 8 15 129 12 160 1.61e-19 88.6
MS.gene25004.t1 AT2G17040 35.185 162 69 5 15 140 9 170 1.61e-19 86.3
MS.gene25004.t1 AT4G36160 35.948 153 59 5 12 128 14 163 1.79e-19 87.4
MS.gene25004.t1 AT3G15510 33.523 176 68 6 16 143 21 195 1.80e-19 87.4
MS.gene25004.t1 AT3G15500 33.140 172 72 7 12 146 14 179 2.56e-19 86.3
MS.gene25004.t1 AT1G61110 34.320 169 62 6 7 128 11 177 2.74e-19 86.3
MS.gene25004.t1 AT3G12977 33.516 182 74 8 12 153 18 192 3.81e-19 85.1
MS.gene25004.t1 AT3G12910 28.832 274 133 11 15 231 24 292 6.45e-19 85.1
MS.gene25004.t1 AT1G34190 38.312 154 51 6 15 129 19 167 1.37e-18 85.9
MS.gene25004.t1 AT4G28530 31.707 164 66 5 12 129 10 173 2.51e-18 84.0
MS.gene25004.t1 AT1G52890 32.768 177 72 7 6 145 8 174 3.18e-18 83.2
MS.gene25004.t1 AT1G01010 37.013 154 60 4 14 130 5 158 3.68e-18 84.0
MS.gene25004.t1 AT4G27410 30.332 211 98 9 12 180 14 217 7.90e-18 82.0
MS.gene25004.t1 AT3G04070 36.923 130 48 4 12 108 10 138 1.34e-17 82.0
MS.gene25004.t1 AT1G32770 34.545 165 57 6 12 128 16 177 2.41e-17 81.3
MS.gene25004.t1 AT1G34180 35.762 151 59 5 15 129 19 167 2.91e-17 81.6
MS.gene25004.t1 AT1G34180 35.762 151 59 5 15 129 19 167 3.58e-17 81.6
MS.gene25004.t1 AT3G10480 44.186 86 39 3 50 128 128 211 5.03e-17 80.9
MS.gene25004.t1 AT2G02450 34.932 146 62 5 15 128 54 198 1.47e-16 79.3
MS.gene25004.t1 AT2G02450 34.932 146 62 5 15 128 54 198 1.52e-16 79.0
MS.gene25004.t1 AT3G10490 46.250 80 34 3 56 128 1 78 1.80e-16 78.6
MS.gene25004.t1 AT3G10490 46.250 80 34 3 56 128 1 78 1.80e-16 78.6
MS.gene25004.t1 AT1G34180 33.129 163 59 5 15 129 19 179 1.13e-15 77.0
MS.gene25004.t1 AT3G12977 38.596 114 55 5 50 153 31 139 1.22e-15 74.7
MS.gene25004.t1 AT1G34180 33.129 163 59 5 15 129 19 179 1.44e-15 76.6
MS.gene25004.t1 AT4G27410 28.070 228 98 9 12 180 14 234 1.62e-15 75.5
MS.gene25004.t1 AT1G56010 42.045 88 40 3 50 128 19 104 5.59e-15 73.2
MS.gene25004.t1 AT1G02250 35.714 154 63 5 13 132 4 155 7.22e-15 73.9
MS.gene25004.t1 AT1G52880 34.545 165 62 6 16 135 21 184 7.00e-14 70.9
MS.gene25004.t1 AT4G10350 41.414 99 49 4 50 141 31 127 1.24e-13 70.1
MS.gene25004.t1 AT5G61430 43.182 88 40 5 50 129 19 104 1.24e-13 69.7
MS.gene25004.t1 AT2G33480 38.636 88 46 3 47 128 18 103 9.75e-13 66.2
MS.gene25004.t1 AT5G64530 33.333 120 51 4 12 108 3 116 6.52e-12 63.5
MS.gene25004.t1 AT5G64530 33.333 120 51 4 12 108 3 116 8.77e-12 62.4

Find 52 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGATTCCATATTTATTTC+TGG 0.145339 8.1:+76961947 None:intergenic
AGGGGTAGCAATAATATTAT+TGG 0.231520 8.1:-76962776 MS.gene25004:CDS
CTTAATCTTTCGATCCTTTC+CGG 0.233046 8.1:+76962802 None:intergenic
AAATATGGAATCAATATTTC+AGG 0.281347 8.1:-76961939 MS.gene25004:CDS
GCATCGTGAAGTGGCGAGTT+TGG 0.289715 8.1:+76961863 None:intergenic
GATGTTGTCATTTCTAAAAT+AGG 0.297612 8.1:+76961841 None:intergenic
GATGATTATTGACGGGGATT+TGG 0.300093 8.1:-76961765 MS.gene25004:CDS
TACTATAGTGGTCGTGTTCC+TGG 0.304206 8.1:-76962734 MS.gene25004:CDS
CGGAAAGGATCGAAAGATTA+AGG 0.317340 8.1:-76962801 MS.gene25004:CDS
TTTAAAAGGACAACTAATAA+TGG 0.327657 8.1:-76962839 MS.gene25004:CDS
GGGGTAGCAATAATATTATT+GGG 0.331309 8.1:-76962775 MS.gene25004:CDS
CAAAGAGTAAGAGGTTTAAA+AGG 0.342416 8.1:-76962853 MS.gene25004:CDS
TTTGAACAATATATATTTAC+CGG 0.353610 8.1:+76961662 None:intergenic
GTCAAGAGTCGGAGGAAATA+AGG 0.354940 8.1:+76963172 None:intergenic
TACTTCGCCAGAAATAAATA+TGG 0.358384 8.1:-76961954 MS.gene25004:CDS
ATACACACTTTAATATCATT+TGG 0.370566 8.1:+76961637 None:intergenic
CATCGTGAAGTGGCGAGTTT+GGG 0.375477 8.1:+76961864 None:intergenic
GGATCGAAAGATTAAGGTTA+GGG 0.381317 8.1:-76962795 MS.gene25004:CDS
TCCTCTTCAATAGTTTCAAA+TGG 0.383569 8.1:+76961806 None:intergenic
ATAAGTATGCAAAGAGTAAG+AGG 0.388091 8.1:-76962862 MS.gene25004:intron
CCAGACCGAGTTGTTGAAAA+TGG 0.393228 8.1:-76961729 MS.gene25004:CDS
TACTTGCATACATCAACTTC+AGG 0.414938 8.1:+76963044 None:intergenic
AAGACTCTTGTTTACTATAG+TGG 0.415807 8.1:-76962746 MS.gene25004:CDS
TTCCTGGTGTTAAAAGCAGC+TGG 0.431255 8.1:-76962718 MS.gene25004:CDS
AGGATCGAAAGATTAAGGTT+AGG 0.440394 8.1:-76962796 MS.gene25004:CDS
TCGGAACTCATGTGTCTACT+TGG 0.451021 8.1:+76962132 None:intergenic
TTGTTGAAAATGGTCTACTA+TGG 0.451094 8.1:-76961719 MS.gene25004:CDS
ACTCTTGACCATGTTCCTGT+TGG 0.471228 8.1:-76963158 MS.gene25004:CDS
GAAACAGATATTTGTGATGA+AGG 0.487684 8.1:-76962159 MS.gene25004:CDS
CTTACAAGCTCTTCATCTGT+TGG 0.488308 8.1:+76963122 None:intergenic
TCAATTCGATGATTATTGAC+GGG 0.491118 8.1:-76961772 MS.gene25004:CDS
TTCAATCTCTGAAAATGAGC+AGG 0.497588 8.1:-76961897 MS.gene25004:CDS
GGTCTGAATCTGAATCCAAC+AGG 0.502719 8.1:+76963143 None:intergenic
AAACAGATATTTGTGATGAA+GGG 0.503954 8.1:-76962158 MS.gene25004:CDS
TCCATTTGAAACTATTGAAG+AGG 0.512914 8.1:-76961807 MS.gene25004:CDS
GTCAATTCGATGATTATTGA+CGG 0.514303 8.1:-76961773 MS.gene25004:CDS
ACAAACCTTGTCATCTTCAA+AGG 0.536374 8.1:+76962670 None:intergenic
CTAAAATAGGCATCGTGAAG+TGG 0.556105 8.1:+76961854 None:intergenic
AGGTATAACATTGTTGACAA+TGG 0.563065 8.1:+76963064 None:intergenic
AACAGATATTTGTGATGAAG+GGG 0.592526 8.1:-76962157 MS.gene25004:CDS
TCCTGGTGTTAAAAGCAGCT+GGG 0.595945 8.1:-76962717 MS.gene25004:CDS
ATTGACGGGGATTTGGACAA+CGG 0.596214 8.1:-76961758 MS.gene25004:CDS
CAATTCGATGATTATTGACG+GGG 0.596678 8.1:-76961771 MS.gene25004:CDS
GTGAAGTGGCGAGTTTGGGA+TGG 0.598328 8.1:+76961868 None:intergenic
TACCACCTTTGAAGATGACA+AGG 0.610149 8.1:-76962675 MS.gene25004:intron
ATCTGAATCCAACAGGAACA+TGG 0.616076 8.1:+76963150 None:intergenic
AACCTTGTCATCTTCAAAGG+TGG 0.630537 8.1:+76962673 None:intergenic
ACAGGAACATGGTCAAGAGT+CGG 0.636129 8.1:+76963161 None:intergenic
TGCTGAAGTAGTCTCTACGC+CGG 0.650251 8.1:-76961681 MS.gene25004:CDS
TATGGAATCAATATTTCAGG+CGG 0.651020 8.1:-76961936 MS.gene25004:CDS
GGAACATGGTCAAGAGTCGG+AGG 0.664688 8.1:+76963164 None:intergenic
GATCGAAAGATTAAGGTTAG+GGG 0.678144 8.1:-76962794 MS.gene25004:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATGAAAAACATTAAAA+AGG + chr8.1:76961887-76961906 None:intergenic 15.0%
!! ATTTCTATTATCATTTATCA+AGG + chr8.1:76962775-76962794 None:intergenic 15.0%
!! TTTGAACAATATATATTTAC+CGG + chr8.1:76963172-76963191 None:intergenic 15.0%
!!! TTTTATGAGAAAATAATGAT+GGG + chr8.1:76961858-76961877 None:intergenic 15.0%
!!! TTTTTATGAGAAAATAATGA+TGG + chr8.1:76961859-76961878 None:intergenic 15.0%
!! AAATATGGAATCAATATTTC+AGG - chr8.1:76962892-76962911 MS.gene25004:intron 20.0%
!! AGAAAAAAGTTTATAAGCAT+TGG - chr8.1:76962531-76962550 MS.gene25004:intron 20.0%
!! TTTAAAAGGACAACTAATAA+TGG - chr8.1:76961992-76962011 MS.gene25004:intron 20.0%
!! TTTATGAGAAAATAATGATG+GGG + chr8.1:76961857-76961876 None:intergenic 20.0%
!! TTTGCATACTTATAATCAAT+AGG + chr8.1:76961962-76961981 None:intergenic 20.0%
!!! ATTGATTCCATATTTATTTC+TGG + chr8.1:76962887-76962906 None:intergenic 20.0%
!!! GAACTTGTATTTGTTTTTAT+AGG - chr8.1:76962844-76962863 MS.gene25004:CDS 20.0%
!!! TTAATATTTTCCTATCTGAT+TGG - chr8.1:76962338-76962357 MS.gene25004:intron 20.0%
!!! TTGTTTGTTTTTTTTTTACC+TGG + chr8.1:76961829-76961848 None:intergenic 20.0%
! AAAACACAAATCACAAACAA+TGG - chr8.1:76962398-76962417 MS.gene25004:intron 25.0%
! AAACAGATATTTGTGATGAA+GGG - chr8.1:76962673-76962692 MS.gene25004:intron 25.0%
!! AAATTTCGAGAACAAAGTTT+TGG - chr8.1:76962791-76962810 MS.gene25004:CDS 25.0%
!! GAAAATCAAGAAACTTTTGA+AGG - chr8.1:76962726-76962745 MS.gene25004:CDS 25.0%
!! GTTCTGAATTTGATGTTTAT+TGG - chr8.1:76962363-76962382 MS.gene25004:intron 25.0%
!!! GATGTTGTCATTTCTAAAAT+AGG + chr8.1:76962993-76963012 None:intergenic 25.0%
AAAAGTCCGCTACAAAATTT+CGG + chr8.1:76962601-76962620 None:intergenic 30.0%
AACAGATATTTGTGATGAAG+GGG - chr8.1:76962674-76962693 MS.gene25004:intron 30.0%
AGGGGTAGCAATAATATTAT+TGG - chr8.1:76962055-76962074 MS.gene25004:intron 30.0%
AGGTATAACATTGTTGACAA+TGG + chr8.1:76961770-76961789 None:intergenic 30.0%
ATAAGTATGCAAAGAGTAAG+AGG - chr8.1:76961969-76961988 MS.gene25004:intron 30.0%
CAAAGAGTAAGAGGTTTAAA+AGG - chr8.1:76961978-76961997 MS.gene25004:intron 30.0%
GAAACAGATATTTGTGATGA+AGG - chr8.1:76962672-76962691 MS.gene25004:intron 30.0%
GGGGTAGCAATAATATTATT+GGG - chr8.1:76962056-76962075 MS.gene25004:intron 30.0%
GTCAATTCGATGATTATTGA+CGG - chr8.1:76963058-76963077 MS.gene25004:CDS 30.0%
TACTTCGCCAGAAATAAATA+TGG - chr8.1:76962877-76962896 MS.gene25004:intron 30.0%
TATGGAATCAATATTTCAGG+CGG - chr8.1:76962895-76962914 MS.gene25004:intron 30.0%
TCAATTCGATGATTATTGAC+GGG - chr8.1:76963059-76963078 MS.gene25004:CDS 30.0%
TCCTCTTCAATAGTTTCAAA+TGG + chr8.1:76963028-76963047 None:intergenic 30.0%
TTGTTGAAAATGGTCTACTA+TGG - chr8.1:76963112-76963131 MS.gene25004:CDS 30.0%
! AAGACTCTTGTTTACTATAG+TGG - chr8.1:76962085-76962104 MS.gene25004:intron 30.0%
! CAAAAGTTTCTTGATTTTCG+TGG + chr8.1:76962725-76962744 None:intergenic 30.0%
! GGACAACTAATAATGGTTTT+TGG - chr8.1:76961999-76962018 MS.gene25004:intron 30.0%
! TATCATCCGAAATTTTGTAG+CGG - chr8.1:76962592-76962611 MS.gene25004:intron 30.0%
! TCCATTTGAAACTATTGAAG+AGG - chr8.1:76963024-76963043 MS.gene25004:intron 30.0%
! TTCAGTTTTTGAATGTTAGC+AGG - chr8.1:76962500-76962519 MS.gene25004:intron 30.0%
!!! GGTTTGTTTTGTTACAACAA+AGG - chr8.1:76962177-76962196 MS.gene25004:CDS 30.0%
!!! GTAACTTGTTTTTGAGATAG+TGG + chr8.1:76961735-76961754 None:intergenic 30.0%
!!! TTCATCTTTTTCAGCTTGTT+CGG - chr8.1:76961898-76961917 MS.gene25004:CDS 30.0%
ACAAACCTTGTCATCTTCAA+AGG + chr8.1:76962164-76962183 None:intergenic 35.0%
AGCTGAGAAAAAAGCTGAAA+AGG - chr8.1:76962644-76962663 MS.gene25004:intron 35.0%
AGGATCGAAAGATTAAGGTT+AGG - chr8.1:76962035-76962054 MS.gene25004:intron 35.0%
ATGTATGCAAGTATGAACCT+TGG - chr8.1:76961797-76961816 MS.gene25004:CDS 35.0%
CAATTCGATGATTATTGACG+GGG - chr8.1:76963060-76963079 MS.gene25004:CDS 35.0%
CTTAATCTTTCGATCCTTTC+CGG + chr8.1:76962032-76962051 None:intergenic 35.0%
GACACAAGAAAAACCATTCT+GGG + chr8.1:76961940-76961959 None:intergenic 35.0%
GATCGAAAGATTAAGGTTAG+GGG - chr8.1:76962037-76962056 MS.gene25004:intron 35.0%
GGATCGAAAGATTAAGGTTA+GGG - chr8.1:76962036-76962055 MS.gene25004:intron 35.0%
TAAAATCAGATGACCCAGAA+TGG - chr8.1:76961924-76961943 MS.gene25004:CDS 35.0%
TACTTGCATACATCAACTTC+AGG + chr8.1:76961790-76961809 None:intergenic 35.0%
TCAGCATGTTTCATGTAGAA+AGG + chr8.1:76962824-76962843 None:intergenic 35.0%
TGTATGCAAGTATGAACCTT+GGG - chr8.1:76961798-76961817 MS.gene25004:CDS 35.0%
TTGAAGGAATTCCTGATGTA+AGG - chr8.1:76962742-76962761 MS.gene25004:CDS 35.0%
! AGTTTCTTGATTTTCGTGGT+CGG + chr8.1:76962721-76962740 None:intergenic 35.0%
! GCTCATTTTCAGAGATTGAA+GGG + chr8.1:76962934-76962953 None:intergenic 35.0%
! GTAGACCATTTTCAACAACT+CGG + chr8.1:76963110-76963129 None:intergenic 35.0%
! TGCTCATTTTCAGAGATTGA+AGG + chr8.1:76962935-76962954 None:intergenic 35.0%
! TTCAATCTCTGAAAATGAGC+AGG - chr8.1:76962934-76962953 MS.gene25004:intron 35.0%
!! AATGGTTTTTGGAAAGCTAC+CGG - chr8.1:76962010-76962029 MS.gene25004:intron 35.0%
!!! AGCATTGGTTTGTTTTGTCT+TGG - chr8.1:76962546-76962565 MS.gene25004:intron 35.0%
AACCTTGTCATCTTCAAAGG+TGG + chr8.1:76962161-76962180 None:intergenic 40.0%
AAGGATGAACTCCTTACATC+AGG + chr8.1:76962756-76962775 None:intergenic 40.0%
ATCTGAATCCAACAGGAACA+TGG + chr8.1:76961684-76961703 None:intergenic 40.0%
CGGAAAGGATCGAAAGATTA+AGG - chr8.1:76962030-76962049 MS.gene25004:intron 40.0%
CTTACAAGCTCTTCATCTGT+TGG + chr8.1:76961712-76961731 None:intergenic 40.0%
GATGATTATTGACGGGGATT+TGG - chr8.1:76963066-76963085 MS.gene25004:CDS 40.0%
GGACACAAGAAAAACCATTC+TGG + chr8.1:76961941-76961960 None:intergenic 40.0%
TTCAGAACAGCCAATCAGAT+AGG + chr8.1:76962351-76962370 None:intergenic 40.0%
! CATTTTCAGAGATTGAAGGG+AGG + chr8.1:76962931-76962950 None:intergenic 40.0%
! CTAAAATAGGCATCGTGAAG+TGG + chr8.1:76962980-76962999 None:intergenic 40.0%
! TACCACCTTTGAAGATGACA+AGG - chr8.1:76962156-76962175 MS.gene25004:CDS 40.0%
! TTTTTACCTGGTAACTCCCA+AGG + chr8.1:76961817-76961836 None:intergenic 40.0%
!! TTTTTGGAAAGCTACCGGAA+AGG - chr8.1:76962015-76962034 MS.gene25004:intron 40.0%
!!! ATCTTTTTCAGCTTGTTCGG+TGG - chr8.1:76961901-76961920 MS.gene25004:CDS 40.0%
ACAGGAACATGGTCAAGAGT+CGG + chr8.1:76961673-76961692 None:intergenic 45.0%
ACTCTTGACCATGTTCCTGT+TGG - chr8.1:76961673-76961692 MS.gene25004:CDS 45.0%
ATTGACGGGGATTTGGACAA+CGG - chr8.1:76963073-76963092 MS.gene25004:CDS 45.0%
CCAGACCGAGTTGTTGAAAA+TGG - chr8.1:76963102-76963121 MS.gene25004:CDS 45.0%
GGTCTGAATCTGAATCCAAC+AGG + chr8.1:76961691-76961710 None:intergenic 45.0%
GTCAAGAGTCGGAGGAAATA+AGG + chr8.1:76961662-76961681 None:intergenic 45.0%
TACTATAGTGGTCGTGTTCC+TGG - chr8.1:76962097-76962116 MS.gene25004:CDS 45.0%
TATGAACCTTGGGAGTTACC+AGG - chr8.1:76961808-76961827 MS.gene25004:CDS 45.0%
TCGGAACTCATGTGTCTACT+TGG + chr8.1:76962702-76962721 None:intergenic 45.0%
! CCATTTTCAACAACTCGGTC+TGG + chr8.1:76963105-76963124 None:intergenic 45.0%
! TCCTGGTGTTAAAAGCAGCT+GGG - chr8.1:76962114-76962133 MS.gene25004:CDS 45.0%
! TTCCTGGTGTTAAAAGCAGC+TGG - chr8.1:76962113-76962132 MS.gene25004:CDS 45.0%
CATCGTGAAGTGGCGAGTTT+GGG + chr8.1:76962970-76962989 None:intergenic 50.0%
TGCTGAAGTAGTCTCTACGC+CGG - chr8.1:76963150-76963169 MS.gene25004:CDS 50.0%
!!! ACCCAGCTGCTTTTAACACC+AGG + chr8.1:76962118-76962137 None:intergenic 50.0%
GCATCGTGAAGTGGCGAGTT+TGG + chr8.1:76962971-76962990 None:intergenic 55.0%
GGAACATGGTCAAGAGTCGG+AGG + chr8.1:76961670-76961689 None:intergenic 55.0%
GTGAAGTGGCGAGTTTGGGA+TGG + chr8.1:76962966-76962985 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 76961652 76963201 76961652 ID=MS.gene25004
chr8.1 mRNA 76961652 76963201 76961652 ID=MS.gene25004.t1;Parent=MS.gene25004
chr8.1 exon 76963055 76963201 76963055 ID=MS.gene25004.t1.exon1;Parent=MS.gene25004.t1
chr8.1 CDS 76963055 76963201 76963055 ID=cds.MS.gene25004.t1;Parent=MS.gene25004.t1
chr8.1 exon 76962676 76962879 76962676 ID=MS.gene25004.t1.exon2;Parent=MS.gene25004.t1
chr8.1 CDS 76962676 76962879 76962676 ID=cds.MS.gene25004.t1;Parent=MS.gene25004.t1
chr8.1 exon 76962095 76962241 76962095 ID=MS.gene25004.t1.exon3;Parent=MS.gene25004.t1
chr8.1 CDS 76962095 76962241 76962095 ID=cds.MS.gene25004.t1;Parent=MS.gene25004.t1
chr8.1 exon 76961652 76961987 76961652 ID=MS.gene25004.t1.exon4;Parent=MS.gene25004.t1
chr8.1 CDS 76961652 76961987 76961652 ID=cds.MS.gene25004.t1;Parent=MS.gene25004.t1
Gene Sequence

>MS.gene25004

ATGACAACCTTATTTCCTCCGACTCTTGACCATGTTCCTGTTGGATTCAGATTCAGACCAACAGATGAAGAGCTTGTAAGCCACTATCTCAAAAACAAGTTACTTGATAACGATTCCATTGTCAACAATGTTATACCTGAAGTTGATGTATGCAAGTATGAACCTTGGGAGTTACCAGGTAAAAAAAAAACAAACAAACAAACCCCATCATTATTTTCTCATAAAAATGCAACCTTTTTAATGTTTTTCATCTTTTTCAGCTTGTTCGGTGGTAAAATCAGATGACCCAGAATGGTTTTTCTTGTGTCCTATTGATTATAAGTATGCAAAGAGTAAGAGGTTTAAAAGGACAACTAATAATGGTTTTTGGAAAGCTACCGGAAAGGATCGAAAGATTAAGGTTAGGGGTAGCAATAATATTATTGGGATTAAGAAGACTCTTGTTTACTATAGTGGTCGTGTTCCTGGTGTTAAAAGCAGCTGGGTGATTCATGAATATCATGATACCACCTTTGAAGATGACAAGGTTTGTTTTGTTACAACAAAGGTTTTATTTGAGTTATTATTTATTTATTTATTTAAATGAATATCATGAAATTTTTTAACTTTAATGTTTTATTAGTTTGTATCAGAGTTATTATTTGTTTATTTAAATGAGTATGACAAAACATGAGTTTATTCATGATTTAATATTTTCCTATCTGATTGGCTGTTCTGAATTTGATGTTTATTGGATTCATTGTTTGAAAACACAAATCACAAACAATGGAACACGATCTTTTATTTACAAAGACTGTTTTTGAGATAAAGTTTTTGTTGAAACGTTATTTTGTTGTAAGTTAATCTTTTTCAGTTTTTGAATGTTAGCAGGACTGTTCAAGAAAAAAGTTTATAAGCATTGGTTTGTTTTGTCTTGGTATTAATTTGTATAAATGCTGATTATCATCCGAAATTTTGTAGCGGACTTTTGTTTTATGTCGCTTGATGAAGAAAGCTGAGAAAAAAGCTGAAAAGGAAGCTGAAACAGATATTTGTGATGAAGGGGAGCCAAGTAGACACATGAGTTCCGACCACGAAAATCAAGAAACTTTTGAAGGAATTCCTGATGTAAGGAGTTCATCCTTGATAAATGATAATAGAAATTTCGAGAACAAAGTTTTGGCTTTAAACCTTTCTACATGAAACATGCTGAGAACTTGTATTTGTTTTTATAGGTGAGTGATGATACTTCGCCAGAAATAAATATGGAATCAATATTTCAGGCGGCAGAAAAATACCTCCCTTCAATCTCTGAAAATGAGCAGGAAGTATCCATCCCAAACTCGCCACTTCACGATGCCTATTTTAGAAATGACAACATCATTGTTCATAGTCCATTTGAAACTATTGAAGAGGAAGATAAGTTCGTCAATTCGATGATTATTGACGGGGATTTGGACAACGGTCATGTCCAGACCGAGTTGTTGAAAATGGTCTACTATGGAAGCAGTGACACAGATGCTGAAGTAGTCTCTACGCCGGTAAATATATATTGTTCAAAGACCAAATGA
Protein sequence

>MS.gene25004.t1

MTTLFPPTLDHVPVGFRFRPTDEELVSHYLKNKLLDNDSIVNNVIPEVDYAKSKRFKRTTNNGFWKATGKDRKIKVRGSNNIIGIKKTLVYYSGRVPGVKSSWVIHEYHDTTFEDDKRTFVLCRLMKKAEKKAEKEAETDICDEGEPSRHMSSDHENQETFEGIPDVSDDTSPEINMESIFQAAEKYLPSISENEQEVSIPNSPLHDAYFRNDNIIVHSPFETIEEEDKFVNSMIIDGDLDNGHVQTELLKMVYYGSSDTDAEVVSTPVNIYCSKTK