AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25115

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25115.t1 MTR_8g020970 98.598 927 13 0 1 927 1 927 0.0 1900
MS.gene25115.t1 MTR_8g020970 98.521 676 10 0 1 676 1 676 0.0 1379
MS.gene25115.t1 MTR_8g080230 54.276 842 377 6 89 926 78 915 0.0 944
MS.gene25115.t1 MTR_4g088810 54.330 843 351 10 90 927 82 895 0.0 915
MS.gene25115.t1 MTR_4g066170 47.753 890 428 13 44 926 46 905 0.0 842
MS.gene25115.t1 MTR_3g479460 48.212 811 403 8 122 926 103 902 0.0 809
MS.gene25115.t1 MTR_3g079450 48.212 811 403 10 122 926 102 901 0.0 804
MS.gene25115.t1 MTR_3g079420 45.972 844 438 10 90 926 32 864 0.0 764
MS.gene25115.t1 MTR_1g083020 45.731 855 453 9 79 926 7 857 0.0 757
MS.gene25115.t1 MTR_8g018420 47.066 835 425 11 99 926 31 855 0.0 753
MS.gene25115.t1 MTR_7g113410 46.753 847 436 13 91 926 24 866 0.0 749
MS.gene25115.t1 MTR_8g018730 47.445 822 411 11 113 926 55 863 0.0 749
MS.gene25115.t1 MTR_8g018735 47.259 821 412 12 114 926 57 864 0.0 746
MS.gene25115.t1 MTR_5g024020 46.780 823 416 13 113 926 57 866 0.0 741
MS.gene25115.t1 MTR_2g099560 47.145 823 411 15 113 926 55 862 0.0 740
MS.gene25115.t1 MTR_2g099570 46.154 845 433 14 91 926 29 860 0.0 738
MS.gene25115.t1 MTR_8g018730 47.253 819 387 12 113 926 55 833 0.0 731
MS.gene25115.t1 MTR_8g018570 46.594 822 420 13 114 926 58 869 0.0 725
MS.gene25115.t1 MTR_8g018520 45.554 821 430 10 113 926 57 867 0.0 720
MS.gene25115.t1 MTR_1g104650 44.457 857 459 12 84 926 2 855 0.0 719
MS.gene25115.t1 MTR_8g020990 45.432 821 431 11 113 926 57 867 0.0 717
MS.gene25115.t1 MTR_8g018690 45.477 818 430 9 113 926 59 864 0.0 714
MS.gene25115.t1 MTR_8g018510 45.455 814 427 11 120 926 65 868 0.0 711
MS.gene25115.t1 MTR_3g081130 46.610 826 412 12 113 926 49 857 0.0 709
MS.gene25115.t1 MTR_3g081130 47.876 777 387 10 156 926 21 785 0.0 704
MS.gene25115.t1 MTR_8g018430 44.580 821 438 11 113 926 50 860 0.0 704
MS.gene25115.t1 MTR_8g018550 46.163 821 425 11 113 926 57 867 0.0 702
MS.gene25115.t1 MTR_5g024020 44.836 823 396 15 113 926 57 830 0.0 690
MS.gene25115.t1 MTR_8g018690 44.377 818 419 10 113 926 59 844 0.0 684
MS.gene25115.t1 MTR_8g018450 43.688 808 408 10 128 926 58 827 0.0 672
MS.gene25115.t1 MTR_8g018590 44.052 765 380 12 177 926 91 822 0.0 634
MS.gene25115.t1 MTR_8g018650 43.286 767 381 9 164 926 90 806 0.0 613
MS.gene25115.t1 MTR_8g018620 43.229 768 380 11 164 926 90 806 0.0 606
MS.gene25115.t1 MTR_8g011340 38.462 832 488 11 65 882 1 822 0.0 573
MS.gene25115.t1 MTR_8g018730 48.727 589 287 8 344 926 4 583 0.0 563
MS.gene25115.t1 MTR_3g079450 46.774 558 285 7 122 676 102 650 1.99e-176 526
MS.gene25115.t1 MTR_8g018735 45.534 571 291 11 114 676 57 615 1.59e-155 472
MS.gene25115.t1 MTR_6g452500 37.908 612 358 9 313 913 11 611 2.74e-133 414
MS.gene25115.t1 MTR_8g021020 46.835 395 207 3 534 926 37 430 2.19e-122 378
MS.gene25115.t1 MTR_7g012030 58.824 119 49 0 578 696 85 203 1.14e-43 157
MS.gene25115.t1 MTR_8g463810 36.000 225 80 2 488 712 197 357 1.33e-39 152
MS.gene25115.t1 MTR_6g046330 53.077 130 50 1 578 696 75 204 4.22e-38 145
MS.gene25115.t1 MTR_4g088805 58.696 92 38 0 836 927 1 92 1.15e-28 110
MS.gene25115.t1 MTR_0248s0010 67.442 43 2 1 732 774 3 33 5.97e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25115.t1 AT1G17420 69.860 929 266 5 1 926 1 918 0.0 1370
MS.gene25115.t1 AT1G72520 70.695 935 255 10 1 926 1 925 0.0 1355
MS.gene25115.t1 AT1G67560 51.348 927 423 14 7 926 11 916 0.0 904
MS.gene25115.t1 AT3G45140 44.470 868 452 14 66 926 51 895 0.0 755
MS.gene25115.t1 AT1G55020 45.745 846 443 13 90 926 20 858 0.0 744
MS.gene25115.t1 AT3G22400 44.664 862 456 11 83 926 27 885 0.0 733
MS.gene25115.t1 AT3G45140 47.500 760 383 10 173 926 4 753 0.0 728

Find 185 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTGAAAATGTGTTCATTT+TGG 0.118061 8.4:-72674359 None:intergenic
TTTACGGTTGACTCAAATTT+TGG 0.128121 8.4:+72672111 MS.gene25115:CDS
GCAAGATCAATTCCTTTATC+TGG 0.137436 8.4:-72673414 None:intergenic
CTTTAGATGCAACTACTAAT+TGG 0.156046 8.4:+72676490 MS.gene25115:CDS
GAGCAGAAAGACTTGATTTA+AGG 0.159246 8.4:-72674420 None:intergenic
ACTCTCTGTCATTGATTATT+AGG 0.161431 8.4:-72678126 None:intergenic
TTTGGTCAGCAATAGAAAAT+TGG 0.164309 8.4:+72678065 MS.gene25115:CDS
TATCAATTCTCTGCGGAAAT+TGG 0.203663 8.4:+72678565 MS.gene25115:CDS
CAGAAGATGGTCTTCTAATT+TGG 0.251107 8.4:+72678047 MS.gene25115:CDS
TCTTGTGGACCATAGATTTC+AGG 0.265813 8.4:-72675555 None:intergenic
GAATTACTTGCTCCTAGTTC+TGG 0.266117 8.4:+72678667 MS.gene25115:CDS
AAGTCCTAACAATCTTAATT+TGG 0.267658 8.4:+72678245 MS.gene25115:CDS
CATTCCAAATTAAGATTGTT+AGG 0.277408 8.4:-72678249 None:intergenic
TACCCTGGCTTGTGGATTCT+TGG 0.281500 8.4:-72674567 None:intergenic
TCGTCTGACGAAGAGTATTT+AGG 0.285117 8.4:+72678487 MS.gene25115:CDS
GAAAAGAAAATTCTCTTTCC+AGG 0.286608 8.4:-72672267 None:intergenic
GATATGAAACTTGTATATTC+AGG 0.290338 8.4:-72678376 None:intergenic
TTGATTATTAGGCTTGGATT+TGG 0.309327 8.4:-72678115 None:intergenic
TCCTCAAATCTTTCATCTCT+TGG 0.311154 8.4:-72674334 None:intergenic
TCAGGATCTAATTTACTAAC+AGG 0.316657 8.4:-72675537 None:intergenic
GTTGCAAGCTACAAAATATA+TGG 0.317917 8.4:+72678438 MS.gene25115:CDS
TTGTCACTGTCGTTCAATTT+CGG 0.321906 8.4:-72678214 None:intergenic
TAATTACACAGCTGAATTTA+CGG 0.322203 8.4:+72672095 MS.gene25115:CDS
AATGAATTCTCAGATGTTGA+TGG 0.326863 8.4:+72674457 MS.gene25115:CDS
GTCAAAGAAGACTAATATTA+AGG 0.327000 8.4:+72672062 MS.gene25115:CDS
ATCCTTCAACACTGCTTCAT+TGG 0.329544 8.4:-72672037 None:intergenic
GTATTAATCTTCTCATCAAC+GGG 0.348087 8.4:-72678339 None:intergenic
AATTTCGGACAGTATCCTTA+TGG 0.355580 8.4:+72678295 MS.gene25115:CDS
CATTTATATTGTCTGCTCAT+AGG 0.356521 8.4:+72676963 MS.gene25115:CDS
CTGTCATTGATTATTAGGCT+TGG 0.359024 8.4:-72678121 None:intergenic
GGATCCAATAACTTGAAAAT+TGG 0.359274 8.4:-72677004 None:intergenic
CAACATGCTGCACTTAATTT+CGG 0.362410 8.4:+72678280 MS.gene25115:CDS
CCACTCACTTGATATAATCT+TGG 0.366511 8.4:-72674608 None:intergenic
CTATGAGCAGACAATATAAA+TGG 0.371741 8.4:-72676962 None:intergenic
GTAAGTCTCTCAATGGTAAC+AGG 0.380176 8.4:-72675504 None:intergenic
TTAGAAAATATAACAATTGA+AGG 0.381425 8.4:+72672183 MS.gene25115:CDS
AGAGGCAACAACCTTCGATT+TGG 0.381540 8.4:+72678512 MS.gene25115:CDS
GTGCTTCATAACTTGCTTCC+TGG 0.384287 8.4:+72674397 MS.gene25115:CDS
GATCTTGGAAACCCAGATAA+AGG 0.388829 8.4:+72673402 MS.gene25115:CDS
GGCCCTAACAAAAGAATTCA+TGG 0.391351 8.4:+72671442 MS.gene25115:CDS
CCATGGTTCGTCTTTCATAT+CGG 0.393755 8.4:-72678188 None:intergenic
AGAATTCATGGGTTCTTCAT+TGG 0.395604 8.4:+72671454 MS.gene25115:CDS
GGTGATGAAGAAGGAAATAT+TGG 0.401225 8.4:-72671485 None:intergenic
TTGAGGAACCGTTGCGGTGC+TGG 0.401483 8.4:+72678631 MS.gene25115:CDS
TTGGTCAGCAATAGAAAATT+GGG 0.402009 8.4:+72678066 MS.gene25115:CDS
GAACTTAGCCTGCCTCCATC+TGG 0.403989 8.4:+72676432 MS.gene25115:CDS
TATGACACATACAATGATCT+TGG 0.412730 8.4:+72673387 MS.gene25115:CDS
AGAAGACCATCTTCTGCATA+AGG 0.416449 8.4:-72678040 None:intergenic
TCTTGGGAATGTCATACTTA+AGG 0.423843 8.4:-72674591 None:intergenic
AAATTAAGATTGTTAGGACT+TGG 0.428810 8.4:-72678243 None:intergenic
TTTAGATGCAACTACTAATT+GGG 0.429267 8.4:+72676491 MS.gene25115:CDS
TGAGTAGTCCAACACTATAA+AGG 0.430450 8.4:-72674480 None:intergenic
TTAGACCATGTGGTTGTGTT+GGG 0.432526 8.4:-72677997 None:intergenic
GATCTTGAGGGAGGCTATCC+TGG 0.433367 8.4:-72677171 None:intergenic
TGATTATTAGGCTTGGATTT+GGG 0.433593 8.4:-72678114 None:intergenic
TGTTGGACTACTCATTAAGT+TGG 0.436827 8.4:+72674489 MS.gene25115:CDS
AGGCTAAGTTCAATTGCAAC+AGG 0.441630 8.4:-72676420 None:intergenic
TCTTTAAGTGCAGACTCTTG+TGG 0.444467 8.4:-72675570 None:intergenic
CACTCACTTGATATAATCTT+GGG 0.446771 8.4:-72674607 None:intergenic
CAAGATCAATTCCTTTATCT+GGG 0.447942 8.4:-72673413 None:intergenic
ATTGAGTCTTGCTTCACTCC+TGG 0.448673 8.4:+72677094 MS.gene25115:CDS
GACCAATGAAGCAGTGTTGA+AGG 0.454038 8.4:+72672035 MS.gene25115:CDS
ATGGAAACAAAAGAGTTGTA+AGG 0.456748 8.4:+72671561 MS.gene25115:CDS
CAAGCTAGAAAAGATCTTCC+TGG 0.457008 8.4:+72672249 MS.gene25115:CDS
TTTGTAGCTTGCAACAAACT+AGG 0.458682 8.4:-72678430 None:intergenic
TTGGTGCACACCAGCATCAT+TGG 0.460866 8.4:-72676544 None:intergenic
TTGAGAAGGTGATTGATAGT+AGG 0.462008 8.4:+72678593 MS.gene25115:CDS
GCTCTAACCTTAAATTTCAC+TGG 0.462917 8.4:-72671695 None:intergenic
GAAAGACTTGATTTAAGGCC+AGG 0.464489 8.4:-72674415 None:intergenic
ACAATTGAAGGATTTGCTAC+TGG 0.466343 8.4:+72672195 MS.gene25115:CDS
AAATTAGATCCTGAAATCTA+TGG 0.468171 8.4:+72675546 MS.gene25115:CDS
GTGGCCTACATTGATTGATT+CGG 0.469250 8.4:-72678164 None:intergenic
AACTCCAGCTATTGCTTGAC+GGG 0.470233 8.4:-72675478 None:intergenic
TCTTCACTGATAGCTGCAAC+AGG 0.474972 8.4:-72671605 None:intergenic
GTTGGACTACTCATTAAGTT+GGG 0.477312 8.4:+72674490 MS.gene25115:CDS
ACCAAGAGATGAAAGATTTG+AGG 0.477798 8.4:+72674333 MS.gene25115:CDS
TTTAGACCATGTGGTTGTGT+TGG 0.478176 8.4:-72677998 None:intergenic
TTTGGAAAGGATCAATGCCT+TGG 0.478720 8.4:+72676335 MS.gene25115:CDS
GATCTTGCTAGACCAACTCT+TGG 0.479530 8.4:+72673429 MS.gene25115:CDS
CCATTCTTCTGTTGAAAACA+TGG 0.484479 8.4:-72671506 None:intergenic
GAAGATGATGATATAGAAGA+AGG 0.486786 8.4:-72671650 None:intergenic
CCATGTGGTTGTGTTGGGTC+TGG 0.492360 8.4:-72677992 None:intergenic
TCATCTCTTGGTATATACAT+AGG 0.492403 8.4:-72674322 None:intergenic
TAACTCCAGCTATTGCTTGA+CGG 0.493184 8.4:-72675479 None:intergenic
CGGCCAGTACGGCACCGTCT+AGG 0.495310 8.4:-72673474 None:intergenic
ATGAAGCAGTGTTGAAGGAT+TGG 0.497542 8.4:+72672040 MS.gene25115:CDS
TACCCTAGACGGTGCCGTAC+TGG 0.498027 8.4:+72673471 MS.gene25115:CDS
GAAGACCATCTTCTGCATAA+GGG 0.501628 8.4:-72678039 None:intergenic
TGCACCAACTTGCCCACCAT+TGG 0.505334 8.4:+72676559 MS.gene25115:CDS
CTAGGAGCAAGTAATTCATA+AGG 0.506030 8.4:-72678661 None:intergenic
CTCAAAAGTTTAATCCCAGC+AGG 0.506743 8.4:-72673294 None:intergenic
GGTATTAATCTTCTCATCAA+CGG 0.507218 8.4:-72678340 None:intergenic
TTTATCACAGTAATTGCTCC+TGG 0.507282 8.4:-72672138 None:intergenic
CATGTTTGCTCCAATGATGC+TGG 0.508665 8.4:+72676534 MS.gene25115:CDS
GGCCGTGAACCATCTGATAC+AGG 0.510574 8.4:+72673492 MS.gene25115:CDS
TACCTGTATCAGATGGTTCA+CGG 0.511896 8.4:-72673494 None:intergenic
CTGCTGCTTACAAAACCAAC+TGG 0.511913 8.4:+72677140 MS.gene25115:CDS
GATCCAAGAATCCACAAGCC+AGG 0.513956 8.4:+72674564 MS.gene25115:CDS
CTTGCTCCTAGTTCTGGACC+TGG 0.515196 8.4:+72678673 MS.gene25115:CDS
GTACGGCACCGTCTAGGGTA+TGG 0.515752 8.4:-72673468 None:intergenic
CTACCCGATGATACACCTGC+TGG 0.515885 8.4:+72673279 MS.gene25115:CDS
GGTGTATCATCGGGTAGATA+AGG 0.517991 8.4:-72673273 None:intergenic
CTTACCAATGGTGGGCAAGT+TGG 0.519041 8.4:-72676563 None:intergenic
GTTGATGGCCTTTATAGTGT+TGG 0.523396 8.4:+72674472 MS.gene25115:CDS
CTTAAGGATACCCTGGCTTG+TGG 0.525590 8.4:-72674575 None:intergenic
ATCTCCATGGCATAGCGACC+AGG 0.526081 8.4:-72677112 None:intergenic
GACCTGGTGTTACATGCAGA+GGG 0.527475 8.4:+72678689 MS.gene25115:CDS
ATATGAAACTTGTATATTCA+GGG 0.530940 8.4:-72678375 None:intergenic
TTTGCCCGTCAAGCAATAGC+TGG 0.532353 8.4:+72675474 MS.gene25115:CDS
ATCCCAGCAGGTGTATCATC+GGG 0.533446 8.4:-72673282 None:intergenic
CACTCCTGGTCGCTATGCCA+TGG 0.533642 8.4:+72677108 MS.gene25115:CDS
TGTTTGGTCCAGATGGAGGC+AGG 0.533655 8.4:-72676440 None:intergenic
ATTTCAGATCCTCCAAGAGT+TGG 0.535524 8.4:-72673441 None:intergenic
CTTGCTAGACCAACTCTTGG+AGG 0.537115 8.4:+72673432 MS.gene25115:CDS
CAACAACCTTCGATTTGGAC+AGG 0.537155 8.4:+72678517 MS.gene25115:CDS
TAATTATTACCTGTATCAGA+TGG 0.538174 8.4:-72673501 None:intergenic
GATTACCCTTATGCAGAAGA+TGG 0.539224 8.4:+72678034 MS.gene25115:CDS
GAAAGGATCAATGCCTTGGA+TGG 0.543205 8.4:+72676339 MS.gene25115:CDS
GTAACACCAGGTCCAGAACT+AGG 0.543782 8.4:-72678679 None:intergenic
TTGCTAACAATGTGAGGCAA+TGG 0.546196 8.4:-72674541 None:intergenic
TTGTGTTTGCTTACCAATGG+TGG 0.547574 8.4:-72676572 None:intergenic
TTGATAGTAGGAATGGTGAT+AGG 0.548139 8.4:+72678605 MS.gene25115:CDS
CTTGGTATATACATAGGAAG+TGG 0.550676 8.4:-72674316 None:intergenic
TCCACACATGAGGTACACTT+TGG 0.551392 8.4:+72677024 MS.gene25115:CDS
GGAATGGTGATAGGAATTTG+AGG 0.553948 8.4:+72678614 MS.gene25115:CDS
GGACCTGGTGTTACATGCAG+AGG 0.558911 8.4:+72678688 MS.gene25115:CDS
AATCCCAGCAGGTGTATCAT+CGG 0.559593 8.4:-72673283 None:intergenic
ACCTGCGGATGAGATCTTGA+GGG 0.562889 8.4:-72677183 None:intergenic
GGCCAGTACGGCACCGTCTA+GGG 0.564885 8.4:-72673473 None:intergenic
GATCGAGTGTTTGGTCCAGA+TGG 0.566962 8.4:-72676447 None:intergenic
TTAGTAGTTGCATCTAAAGC+AGG 0.568173 8.4:-72676486 None:intergenic
TTTGGCACGTAACCTCCATA+AGG 0.568931 8.4:-72678310 None:intergenic
TGAAAACATGGTGATGAAGA+AGG 0.569969 8.4:-72671494 None:intergenic
TCCCTCAAGATCTCATCCGC+AGG 0.574537 8.4:+72677182 MS.gene25115:CDS
GGATCTAATTTACTAACAGG+TGG 0.574715 8.4:-72675534 None:intergenic
GTGCAGAGGATAAATTTGCT+TGG 0.574838 8.4:+72675436 MS.gene25115:intron
GATCTAATTTACTAACAGGT+GGG 0.574850 8.4:-72675533 None:intergenic
GCTACGCACACATGCTTGCA+TGG 0.575061 8.4:+72676937 MS.gene25115:CDS
AATCTGAGAGGTGATGGAAA+AGG 0.575229 8.4:+72673333 MS.gene25115:CDS
GAAAACTGTAAGTCTCTCAA+TGG 0.578316 8.4:-72675511 None:intergenic
ATCCTGGTCAAAACGCCAGT+TGG 0.579614 8.4:-72677155 None:intergenic
AAATGTATCCATACCCTAGA+CGG 0.586694 8.4:+72673460 MS.gene25115:CDS
ATCCAAGAATCCACAAGCCA+GGG 0.589581 8.4:+72674565 MS.gene25115:CDS
TTCGGACAGTATCCTTATGG+AGG 0.590508 8.4:+72678298 MS.gene25115:CDS
ATTATTAGCTTTAGACCATG+TGG 0.590586 8.4:-72678007 None:intergenic
TACCCGATGATACACCTGCT+GGG 0.591254 8.4:+72673280 MS.gene25115:CDS
TACCTGCGGATGAGATCTTG+AGG 0.594715 8.4:-72677184 None:intergenic
CAGATGGTTCACGGCCAGTA+CGG 0.595137 8.4:-72673485 None:intergenic
AGCAGCAGAACTAATCTCCA+TGG 0.597060 8.4:-72677125 None:intergenic
TGGATCTTGCTAACAATGTG+AGG 0.598996 8.4:-72674547 None:intergenic
GCATCACCTGTCCAAATCGA+AGG 0.600030 8.4:-72678523 None:intergenic
AAGGTGATTGATAGTAGGAA+TGG 0.600577 8.4:+72678598 MS.gene25115:CDS
CAGAGCTTGATCAATGCAGA+TGG 0.602184 8.4:+72677067 MS.gene25115:CDS
TGTGTTTGCTTACCAATGGT+GGG 0.607789 8.4:-72676571 None:intergenic
TCGTTCAATTTCGGCCACCA+TGG 0.607867 8.4:-72678205 None:intergenic
TGTCATACTTAAGGATACCC+TGG 0.608790 8.4:-72674582 None:intergenic
TCCAAAGTGTACCTCATGTG+TGG 0.612282 8.4:-72677025 None:intergenic
CGAGTGTTTGGTCCAGATGG+AGG 0.614080 8.4:-72676444 None:intergenic
GCCCTAACAAAAGAATTCAT+GGG 0.614296 8.4:+72671443 MS.gene25115:CDS
TTTAAATGGTACCTCTTGGA+CGG 0.616838 8.4:-72675625 None:intergenic
ACCCCTCTGCATGTAACACC+AGG 0.617716 8.4:-72678691 None:intergenic
ATTAAGTGCAGCATGTTGCG+CGG 0.618193 8.4:-72678275 None:intergenic
ATGCAACTACTAATTGGGTG+TGG 0.623131 8.4:+72676496 MS.gene25115:CDS
ACGAAGAGTATTTAGGTGAG+AGG 0.628094 8.4:+72678494 MS.gene25115:CDS
ATGACAGAGAGTTACAAGCT+TGG 0.632533 8.4:+72678137 MS.gene25115:CDS
TACTCCGAATCAATCAATGT+AGG 0.641645 8.4:+72678160 MS.gene25115:CDS
GCAAAACACCAGCACCGCAA+CGG 0.642856 8.4:-72678639 None:intergenic
GCTTGAACCCAAGAATTGCA+AGG 0.647001 8.4:-72672231 None:intergenic
GGAAATTGGAAAGATTGAGA+AGG 0.652309 8.4:+72678579 MS.gene25115:CDS
AGGAATTTGAGGAACCGTTG+CGG 0.653263 8.4:+72678625 MS.gene25115:CDS
TATTACTTGTGAGAGTCGTG+TGG 0.661476 8.4:+72674288 MS.gene25115:CDS
CCGATATGAAAGACGAACCA+TGG 0.663394 8.4:+72678188 MS.gene25115:CDS
TAATGGCATGACCGTCCAAG+AGG 0.670114 8.4:+72675614 MS.gene25115:CDS
TGCGGATGAGATCTTGAGGG+AGG 0.673318 8.4:-72677180 None:intergenic
AGTGAGGAACAAGATCAAAG+AGG 0.677524 8.4:+72671727 MS.gene25115:CDS
AGTTATTGGATCCACACATG+AGG 0.685129 8.4:+72677014 MS.gene25115:CDS
GTTCGTCTTTCATATCGGCG+TGG 0.688334 8.4:-72678183 None:intergenic
CCAGACCCAACACAACCACA+TGG 0.693358 8.4:+72677992 MS.gene25115:CDS
ACCTGGTGTTACATGCAGAG+GGG 0.702111 8.4:+72678690 MS.gene25115:CDS
AGTAATAGTTCACATAAGTG+CGG 0.704279 8.4:-72678090 None:intergenic
ATTAATCTTCTCATCAACGG+GGG 0.722560 8.4:-72678337 None:intergenic
ATATGAAAGACGAACCATGG+TGG 0.746586 8.4:+72678191 MS.gene25115:CDS
ACAGGCTTTAGAGTACCAAG+TGG 0.757581 8.4:-72676402 None:intergenic
TTAGAGCTGTTGTAACAGTG+AGG 0.760433 8.4:+72671711 MS.gene25115:CDS
TATTAATCTTCTCATCAACG+GGG 0.765013 8.4:-72678338 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.4 gene 72671440 72678735 72671440 ID=MS.gene25115
chr8.4 mRNA 72671440 72678735 72671440 ID=MS.gene25115.t1;Parent=MS.gene25115
chr8.4 exon 72671440 72671845 72671440 ID=MS.gene25115.t1.exon1;Parent=MS.gene25115.t1
chr8.4 CDS 72671440 72671845 72671440 ID=cds.MS.gene25115.t1;Parent=MS.gene25115.t1
chr8.4 exon 72672016 72672296 72672016 ID=MS.gene25115.t1.exon2;Parent=MS.gene25115.t1
chr8.4 CDS 72672016 72672296 72672016 ID=cds.MS.gene25115.t1;Parent=MS.gene25115.t1
chr8.4 exon 72673273 72673513 72673273 ID=MS.gene25115.t1.exon3;Parent=MS.gene25115.t1
chr8.4 CDS 72673273 72673513 72673273 ID=cds.MS.gene25115.t1;Parent=MS.gene25115.t1
chr8.4 exon 72674287 72674622 72674287 ID=MS.gene25115.t1.exon4;Parent=MS.gene25115.t1
chr8.4 CDS 72674287 72674622 72674287 ID=cds.MS.gene25115.t1;Parent=MS.gene25115.t1
chr8.4 exon 72675442 72675635 72675442 ID=MS.gene25115.t1.exon5;Parent=MS.gene25115.t1
chr8.4 CDS 72675442 72675635 72675442 ID=cds.MS.gene25115.t1;Parent=MS.gene25115.t1
chr8.4 exon 72676279 72676580 72676279 ID=MS.gene25115.t1.exon6;Parent=MS.gene25115.t1
chr8.4 CDS 72676279 72676580 72676279 ID=cds.MS.gene25115.t1;Parent=MS.gene25115.t1
chr8.4 exon 72676937 72677203 72676937 ID=MS.gene25115.t1.exon7;Parent=MS.gene25115.t1
chr8.4 CDS 72676937 72677203 72676937 ID=cds.MS.gene25115.t1;Parent=MS.gene25115.t1
chr8.4 exon 72677979 72678735 72677979 ID=MS.gene25115.t1.exon8;Parent=MS.gene25115.t1
chr8.4 CDS 72677979 72678735 72677979 ID=cds.MS.gene25115.t1;Parent=MS.gene25115.t1
Gene Sequence

>MS.gene25115

ATGGCCCTAACAAAAGAATTCATGGGTTCTTCATTGGTAGAGAGACCAATATTTCCTTCTTCATCACCATGTTTTCAACAGAAGAATGGTGGATTCTTGATCAGTCCTGTTTTTGTTCCTTATGGAAACAAAAGAGTTGTAAGGTTGAGAAAATCTGCAAAGTTTCCTGTTGCAGCTATCAGTGAAGATTTGATGAAAAACTCTTCTTCACCTTCTTCTATATCATCATCTTCTTCTTCTGTTCCTGCTGAAAAACCAGTGAAATTTAAGGTTAGAGCTGTTGTAACAGTGAGGAACAAGATCAAAGAGGATTTCAAAGAGACTTTTGTGAAACATCTTGATGCTTTTACTGATAGGATTGGAAGAAATGTTGTTCTTGAACTTTTTAGCATTGAGATTGATCCAAGTAAGATTTTTCTGTAGTGTGTTAATTAAAAATCAATATTTACTTTATACACTTAATTTTAATTTCTTTGATTTTGATAATTTTTAGTTCTTTATGAAATTTTATTTGGATTAGCTTTAAATTTATAACTGAATTAAATTATTGGTGATTTTTATGTTGTGATCTTTCAGAAACAAAAGCTGCAAAGAAGACCAATGAAGCAGTGTTGAAGGATTGGTCAAAGAAGACTAATATTAAGGCAGAGAGAGTTAATTACACAGCTGAATTTACGGTTGACTCAAATTTTGGAGAACCAGGAGCAATTACTGTGATAAATAATCATCAACAAGAATTTTACTTAGAAAATATAACAATTGAAGGATTTGCTACTGGAGCATTTCATTTTCCTTGCAATTCTTGGGTTCAAGCTAGAAAAGATCTTCCTGGAAAGAGAATTTTCTTTTCTAATAAGGTATCTAATAATTAATTCTTCAACTCTTTTTTTGTTTTTTTTGTTATTTATCTGTCATCATAACATGGCCCAGTAAGGAAAAAAAAGGTAGTACAGCCAAAAAAATAGTATCATAAGGCAACAATATTTGTTTATTCATTTTTTCAACGTGAGAAATAAGGTGAAAATTGAAAGTTAAAAGGTATAATAATTTTTGTTTTGAAGAATCTTGAAAAGGCACAATAATTCCAAAAGTTCATTTTACCGTAAAAATATTGAATGTTATTTAATAATTATTTTATGTGCCAAAACCAAGCAGTCCATGATTTTATAATTATTGTTATATATCATTGCATTTTTTAATTAATTATCCACGATAAAGTGATATACTAATAAACAATTAACCTAGAAAATCATATAGTCAAATTTATGCATATGGACCAAGCACCATTGAATTGACCTGACGACAACCTACGGTATGAATTGGGCAAAGAAACCGTTGAATTGAATACTAGTAGTACTTGTCAAGAAAAAAAAAATAATACTAGCCAATACCCTCTTTATCTTTATTTTTCGAGTCAACAACATTCTCTAACACTCACTCGTTTATTGGATTAAATTAAGGTGGGTCTCACACGTTAAAAATTGATTTTCTTAAAGTGATGAAACATATATTGATTTCATCTAACAAGAGAGTGAGTATTAGAAACTATTAAAAAGAAAGTATCTCTCGCATTTCTCTTATTTTTCTGACTAGTACTAGTAGTCTTTATGGTCGAGGATGAATGTGGAGCAATAATCTTACCCTCGGCAATATTCAGCATTTCCCTAATGTAAATTTATACAAATAATAAAGTGGTAACATTCCCTAAATATAAAAATAAAGCGGTAACATTTACCTTTCTCGATCTTTTGACTAAGATGTGTAGTATATATATTCTTTTTGGAATAAAGTGGTAACATATGTTTGATTTAATGTGCGATTTACTTTGAACAGCCTTATCTACCCGATGATACACCTGCTGGGATTAAACTTTTGAGAGAGAAAGACCTGAAAAATCTGAGAGGTGATGGAAAAGGAGTTAGAAAATTATCTGACAGAATATATGATTATGACACATACAATGATCTTGGAAACCCAGATAAAGGAATTGATCTTGCTAGACCAACTCTTGGAGGATCTGAAATGTATCCATACCCTAGACGGTGCCGTACTGGCCGTGAACCATCTGATACAGGTAATAATTAATTAAATAAGCTATTTTACACTACTAATGATTAATGAATATTGCATTCTATCCTTTAGTAGGGTGTTAAACTATATTTCTATCCTTTAAAGTGCGATGTTAGATGATCTGGGTTCAAATCCCGACTATTGTGATTTCATCTATCTACAAAAGAAGTGTGTCGTTAGATTACAATATGAAATTATAAGTTGTCCTCGTTTTAGTTGCACATTGTATTCTAGGTTGTATTTAAAGTTATACATCGTGGTCTTGTAAATACAGCTGAGATGATAAAATTCTTAAATGTTGCAATGACATTTGATATGATGGGGCGTGGGATTAAACCAACATTTCTTCACTTCTTTACAATTAGTGTATCTACGTACGCGTGAATTTCACCGCTAATCTCCCTAGGAAAAAAAGTGATAGTTTGGAAATATAAAAACTTATTTTACATTTAAATTTAGTAATTTTGTACAAATCAATTTGATTTTAAGGATTAAAATTTATTGACTAATTTGATGTATTTTCTTACAGAGGATCATATCTAGACCAACTTGTGGGCACCGACATTTTGTGATGTTTTATAGTGGTGTCATGCCATGCTATAATATTATTTTCTAAAATATTTGTACAAATGTTTACTTGATAAAATATGATTACCCTGAGTAAAATTATTTTATACTGTCAATAGATTGCAATTTAATTTGAATTTAAGATAATATGTTACTTTGCTTATGGTGATTCTAAATAATTAAGCAAAATTGAATTGGATGGTGCTGCAGATATTACTTGTGAGAGTCGTGTGGAGAAGCCACTTCCTATGTATATACCAAGAGATGAAAGATTTGAGGAATCCAAAATGAACACATTTTCAGTGAAGAGGCTAAAAGGAGTGCTTCATAACTTGCTTCCTGGCCTTAAATCAAGTCTTTCTGCTCAAAACAGAGATTTCAATGAATTCTCAGATGTTGATGGCCTTTATAGTGTTGGACTACTCATTAAGTTGGGTTTGCAAGATGATATTTTGAAGAAACTTCCATTGCCTCACATTGTTAGCAAGATCCAAGAATCCACAAGCCAGGGTATCCTTAAGTATGACATTCCCAAGATTATATCAAGTGAGTGGCTACATAATTTATACTTGTTATTGTCGATTTCTTGCTTAAAAAATTGGCTACATAATTTTTCATAAGTAAATTTTTGGTTTAAATACACTTTTAGTCTTCTCGTCCTCACAATTTTGATTCTACATTTTTTAAAAATTTTGAAAAAATTCCGGATTACTAAATTTTGGGCTTGTTTTTACTAATGCTCCAGGTTGAATAATGAATTAACAACTCATTAGATGGAAAATGTAGCATTGTATAATTTTTGAGATATAATTTTCTTAGGGTTTTACCCTTTCTTCATTTAGAAAATTTCCTCTTTGTATAAAATTTCTAAACTTTATATTTCCAAATTGAATTATGTCTTTATAAAAAATTTAACTTAGATTTGAATATTTATACTTTTTTTTACTTTCCCTTTCATTTGCAATGTGGTGGGGTCTTTGGCCATGAATATTCTATAATGGAATGCAAGCATGCCATATGAAAAGATAGTGCTACAAATTAGAGAGTGCCACACTACAACATGTTATTTGGCTTTTAGTGTTTGTCAGTACAATTTCACTCCAAAAAGATGAGTTTTCAGTTCAAAGCTAGTAGATCTAATGTTTATACCATTCCTTTTATATATAAAAAAATTACAACTGGGCCAATTTGTTATTAATTATTACTTGTTCATTTGGAACTAAACTGCCACCAAAGCTGTACTAATCAATTGTTAATTTTAAATGTTTCACATGTGTGTCTAGTTATTATAAACTGTTGATATAAAAATTAAAGTTCTTGTTTAATTAATTGTGAAATTATTGATCTCATGTGCCTAATGTGCAGAGGATAAATTTGCTTGGTTGAGAGATGATGAATTTGCCCGTCAAGCAATAGCTGGAGTTAATCCTGTTACCATTGAGAGACTTACAGTTTTCCCACCTGTTAGTAAATTAGATCCTGAAATCTATGGTCCACAAGAGTCTGCACTTAAAGAAGAGCACATTTTAAATCAACTTAATGGCATGACCGTCCAAGAGGTACCATTTAAAGCATTCAAAAACTTTTTCCTTTTTGGTACAATGCATTCATAAACTTACTAACCATGTTACCTAATCAAGTGCCTTGTGAATTGTTTTTTCAAACTATTTAACCAATTATAACATTTCCCATGTACCTTAAAAATAAACATATTTCTCATGGATTAATTTTTTCACTTGACATCAAATTTATAATGGTCGCCAATACGTGGTTTGGTTCAAGTAGTGAGTGATTTGGATACTTTAAACAAATGGTCAGAGATTTGACTACTCAGGATCGACCTAAAGACTAAGCAATTTAAGTTAGGGTTTTAAGTCTCACTTCAAAATTTGTTTTAGACTTCTAAACATATTGTGTACGTCCTGAGGCAACTAACTCTTGTATATGGAGAAAAATCAGTTAAAAATGGCGAATCTATCATGGTGCCCAATAGGTTCCAACAAAAATTACCATTGTTAAAGGACAATGATACTTCGTGCCTATAACTTGATTTTAAAGAAATAAATTTAAAATTATCCTATGTATGCTCTTGAATGCACAATTCAATACTAGTAGATCATATAAATTTTGACATTTTTTTTTTATTAATTTACTTATTTGCACTAAACTCCCACTTTCTTTTCTTCTTTTGGAATTTTGCAGGCAATAGATCAAAATAAGTTGTTTATAATTGATTATCATGATATCTACCTTCCATTTTTGGAAAGGATCAATGCCTTGGATGGTAGAAAATCATATGCCACACGTACCATATACTTTTTGACACCACTTGGTACTCTAAAGCCTGTTGCAATTGAACTTAGCCTGCCTCCATCTGGACCAAACACTCGATCAAAACGTGTAGTTACACCTGCTTTAGATGCAACTACTAATTGGGTGTGGATGCTTGCAAAAGCTCATGTTTGCTCCAATGATGCTGGTGTGCACCAACTTGCCCACCATTGGTAAGCAAACACAATTTTTCATTTACTTAGTCAATCATCAATTTAGTCCTAAAATTTTATGTGACAAGTGACACAAGACATTTACACTCACACATGGACACTGATAGTAATTTAATGAAATAATTGAATATAATCACATATGTTGTATCAAATTGACATGTGTCGGACACAAAATATGTCTTTCATGTGAAAAGACATTGCGACATAGGTTTGAAAAAATTGTTTAGAAATTATTTTGCAATTTTGTTAGTTTTATTGACACAATACCTTGACACATGCAACTTAAATACTAAATTGGTGATTGAAGACATGAACTTATTTCTTTGCTTATTTTTTCTCGGTGAATTTTTATATAGGCTACGCACACATGCTTGCATGGAACCATTTATATTGTCTGCTCATAGGCAATTAAGTGCAATGCATCCAATTTTCAAGTTATTGGATCCACACATGAGGTACACTTTGGAGATCAATGCTTTAGCTCGACAGAGCTTGATCAATGCAGATGGTGTCATTGAGTCTTGCTTCACTCCTGGTCGCTATGCCATGGAGATTAGTTCTGCTGCTTACAAAACCAACTGGCGTTTTGACCAGGATAGCCTCCCTCAAGATCTCATCCGCAGGTAAATATCATATATTAATTTCGGCATTTTTTTTCCGCTTTGTTTTAAAGATCCTAGAACGAAACTCACACACGCAATACGGAGAATAGACATATTTTTTGCTTCTAACCTCTGTACATCAATTGTTTTCGCTGCCTTTTACCATTTGAGGCGCATTGTTGGTTCGTTATAACTTATTAGTTTATGACCTCAACTCTAAAGTTTAAAATAAGGGTCTGTGATTGTGGTCTGTGATTGTATCAGCTTCGTTTAACTACGATTCTTTGCAATATTAAGGATCGTGACATCACCACTCTCTAAAACCCTCCTCAATTATATTTTGATACCTAAGAGAAATTGTAAATATTAAACTTTCTTTTATGATTTGTTTGAGGCGTCACTTAGTATATGTTTGATTTCACTTTAGTCAAAATTGATTCTAGAAGTGTATAATTGTTTTTTATATGTTTGAGTGCATTTCAGAATAATTGATTTTGACTCTAGAATTGATTTTAATTTGAAACTATGATTTATAGTTTTTGACTCTAAACGTTAATTTTACACTTAAATTTATTGTTCAATCCACTATATGTGAATTTGTCCAAACGTAAACCATTTTACATTCAACTTAGTTTTAACTAAAATCAATTTTTCTCACCATAAAATCAAATACACACCTAGCTTCTACGGAAAAGAGACCCTACACTTTTCTTAACATGTAATTGATGTTTGCTTTATTTGGTGCAAGCATATGACATATAAATCTAACTAAGATTTTCACTTTTTAACATTTCAGGGGAATGGCAGTACCAGACCCAACACAACCACATGGTCTAAAGCTAATAATGAAAGATTACCCTTATGCAGAAGATGGTCTTCTAATTTGGTCAGCAATAGAAAATTGGGTCCGCACTTATGTGAACTATTACTACCCAAATCCAAGCCTAATAATCAATGACAGAGAGTTACAAGCTTGGTACTCCGAATCAATCAATGTAGGCCACGCCGATATGAAAGACGAACCATGGTGGCCGAAATTGAACGACAGTGACAATCTTGTCCAAGTCCTAACAATCTTAATTTGGAATGCATCCGCGCAACATGCTGCACTTAATTTCGGACAGTATCCTTATGGAGGTTACGTGCCAAATCGTCCCCCGTTGATGAGAAGATTAATACCAGAAGAAAATGACCCTGAATATACAAGTTTCATATCTGATCCACAAAAGTATTTTCTCAATGCATTGCCTAGTTTGTTGCAAGCTACAAAATATATGGCTGTTGTTGACACGCTTTCGACTCATTCGTCTGACGAAGAGTATTTAGGTGAGAGGCAACAACCTTCGATTTGGACAGGTGATGCTGAGATTGTCGAAGCATTTTATCAATTCTCTGCGGAAATTGGAAAGATTGAGAAGGTGATTGATAGTAGGAATGGTGATAGGAATTTGAGGAACCGTTGCGGTGCTGGTGTTTTGCCTTATGAATTACTTGCTCCTAGTTCTGGACCTGGTGTTACATGCAGAGGGGTTCCAAATAGTGTGTCCACATAA
Protein sequence

>MS.gene25115.t1

MALTKEFMGSSLVERPIFPSSSPCFQQKNGGFLISPVFVPYGNKRVVRLRKSAKFPVAAISEDLMKNSSSPSSISSSSSSVPAEKPVKFKVRAVVTVRNKIKEDFKETFVKHLDAFTDRIGRNVVLELFSIEIDPKTKAAKKTNEAVLKDWSKKTNIKAERVNYTAEFTVDSNFGEPGAITVINNHQQEFYLENITIEGFATGAFHFPCNSWVQARKDLPGKRIFFSNKPYLPDDTPAGIKLLREKDLKNLRGDGKGVRKLSDRIYDYDTYNDLGNPDKGIDLARPTLGGSEMYPYPRRCRTGREPSDTDITCESRVEKPLPMYIPRDERFEESKMNTFSVKRLKGVLHNLLPGLKSSLSAQNRDFNEFSDVDGLYSVGLLIKLGLQDDILKKLPLPHIVSKIQESTSQGILKYDIPKIISKDKFAWLRDDEFARQAIAGVNPVTIERLTVFPPVSKLDPEIYGPQESALKEEHILNQLNGMTVQEAIDQNKLFIIDYHDIYLPFLERINALDGRKSYATRTIYFLTPLGTLKPVAIELSLPPSGPNTRSKRVVTPALDATTNWVWMLAKAHVCSNDAGVHQLAHHWLRTHACMEPFILSAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLINADGVIESCFTPGRYAMEISSAAYKTNWRFDQDSLPQDLIRRGMAVPDPTQPHGLKLIMKDYPYAEDGLLIWSAIENWVRTYVNYYYPNPSLIINDRELQAWYSESINVGHADMKDEPWWPKLNDSDNLVQVLTILIWNASAQHAALNFGQYPYGGYVPNRPPLMRRLIPEENDPEYTSFISDPQKYFLNALPSLLQATKYMAVVDTLSTHSSDEEYLGERQQPSIWTGDAEIVEAFYQFSAEIGKIEKVIDSRNGDRNLRNRCGAGVLPYELLAPSSGPGVTCRGVPNSVST