AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051115

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051115.t1 MTR_8g020970 98.813 927 11 0 1 927 1 927 0.0 1903
MS.gene051115.t1 MTR_8g020970 98.817 676 8 0 1 676 1 676 0.0 1383
MS.gene051115.t1 MTR_8g080230 54.276 842 377 6 89 926 78 915 0.0 945
MS.gene051115.t1 MTR_4g088810 54.448 843 350 10 90 927 82 895 0.0 919
MS.gene051115.t1 MTR_4g066170 48.562 869 430 11 64 926 48 905 0.0 844
MS.gene051115.t1 MTR_3g479460 48.212 811 403 8 122 926 103 902 0.0 810
MS.gene051115.t1 MTR_3g079450 48.212 811 403 10 122 926 102 901 0.0 805
MS.gene051115.t1 MTR_3g079420 45.972 844 438 10 90 926 32 864 0.0 765
MS.gene051115.t1 MTR_1g083020 45.848 855 452 9 79 926 7 857 0.0 757
MS.gene051115.t1 MTR_8g018420 47.122 834 424 11 100 926 32 855 0.0 754
MS.gene051115.t1 MTR_8g018730 47.567 822 410 11 113 926 55 863 0.0 751
MS.gene051115.t1 MTR_7g113410 46.753 847 436 13 91 926 24 866 0.0 751
MS.gene051115.t1 MTR_8g018735 47.381 821 411 12 114 926 57 864 0.0 748
MS.gene051115.t1 MTR_5g024020 46.902 823 415 13 113 926 57 866 0.0 744
MS.gene051115.t1 MTR_2g099560 47.145 823 411 15 113 926 55 862 0.0 741
MS.gene051115.t1 MTR_2g099570 46.154 845 433 14 91 926 29 860 0.0 738
MS.gene051115.t1 MTR_8g018730 47.375 819 386 12 113 926 55 833 0.0 733
MS.gene051115.t1 MTR_8g018570 46.594 822 420 13 114 926 58 869 0.0 725
MS.gene051115.t1 MTR_8g018520 45.676 821 429 10 113 926 57 867 0.0 722
MS.gene051115.t1 MTR_1g104650 44.574 857 458 12 84 926 2 855 0.0 721
MS.gene051115.t1 MTR_8g020990 45.432 821 431 11 113 926 57 867 0.0 716
MS.gene051115.t1 MTR_8g018690 45.477 818 430 9 113 926 59 864 0.0 714
MS.gene051115.t1 MTR_8g018510 45.455 814 427 11 120 926 65 868 0.0 711
MS.gene051115.t1 MTR_3g081130 46.610 826 412 12 113 926 49 857 0.0 709
MS.gene051115.t1 MTR_8g018430 44.580 821 438 11 113 926 50 860 0.0 705
MS.gene051115.t1 MTR_3g081130 47.938 776 386 10 157 926 22 785 0.0 705
MS.gene051115.t1 MTR_8g018550 46.163 821 425 11 113 926 57 867 0.0 702
MS.gene051115.t1 MTR_5g024020 44.957 823 395 15 113 926 57 830 0.0 691
MS.gene051115.t1 MTR_8g018690 44.377 818 419 10 113 926 59 844 0.0 684
MS.gene051115.t1 MTR_8g018450 43.688 808 408 10 128 926 58 827 0.0 673
MS.gene051115.t1 MTR_8g018590 44.052 765 380 12 177 926 91 822 0.0 634
MS.gene051115.t1 MTR_8g018650 43.286 767 381 9 164 926 90 806 0.0 613
MS.gene051115.t1 MTR_8g018620 43.229 768 380 11 164 926 90 806 0.0 606
MS.gene051115.t1 MTR_8g011340 39.371 795 460 10 102 882 36 822 0.0 574
MS.gene051115.t1 MTR_8g018730 48.727 589 287 8 344 926 4 583 0.0 563
MS.gene051115.t1 MTR_3g079450 46.774 558 285 7 122 676 102 650 1.34e-176 527
MS.gene051115.t1 MTR_8g018735 45.709 571 290 11 114 676 57 615 2.19e-156 474
MS.gene051115.t1 MTR_6g452500 37.908 612 358 9 313 913 11 611 2.71e-133 414
MS.gene051115.t1 MTR_8g021020 46.835 395 207 3 534 926 37 430 2.88e-122 378
MS.gene051115.t1 MTR_7g012030 58.824 119 49 0 578 696 85 203 1.09e-43 157
MS.gene051115.t1 MTR_8g463810 36.000 225 80 2 488 712 197 357 1.31e-39 152
MS.gene051115.t1 MTR_6g046330 53.077 130 50 1 578 696 75 204 4.14e-38 145
MS.gene051115.t1 MTR_4g088805 58.696 92 38 0 836 927 1 92 1.13e-28 110
MS.gene051115.t1 MTR_0248s0010 67.442 43 2 1 732 774 3 33 5.97e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051115.t1 AT1G17420 70.119 927 267 5 1 926 1 918 0.0 1375
MS.gene051115.t1 AT1G72520 70.909 935 253 10 1 926 1 925 0.0 1359
MS.gene051115.t1 AT1G67560 51.348 927 423 13 7 926 11 916 0.0 907
MS.gene051115.t1 AT3G45140 44.470 868 452 14 66 926 51 895 0.0 757
MS.gene051115.t1 AT1G55020 45.745 846 443 13 90 926 20 858 0.0 746
MS.gene051115.t1 AT3G22400 44.664 862 456 11 83 926 27 885 0.0 733
MS.gene051115.t1 AT3G45140 47.500 760 383 10 173 926 4 753 0.0 728

Find 184 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTGAAAATGTGTTCATTT+TGG 0.118061 8.3:-70246651 None:intergenic
TTTACGGTTGACTCAAATTT+TGG 0.128121 8.3:+70243735 MS.gene051115:CDS
GCAAGATCAATTCCTTTATC+TGG 0.137436 8.3:-70245062 None:intergenic
CTTTAGATGCAACTACTAAT+TGG 0.156046 8.3:+70248999 MS.gene051115:CDS
GAGCAGAAAGACTTGATTTA+AGG 0.159246 8.3:-70246712 None:intergenic
ACTCTCTGTCATTGATTATT+AGG 0.161431 8.3:-70250663 None:intergenic
TTTGGTCAGCAATAGAAAAT+TGG 0.164309 8.3:+70250602 MS.gene051115:CDS
TATCAATTCTCTGCGGAAAT+TGG 0.203663 8.3:+70251102 MS.gene051115:CDS
CAGAAGATGGTCTTCTAATT+TGG 0.251107 8.3:+70250584 MS.gene051115:CDS
TCTTGTGGACCATAGATTTC+AGG 0.265813 8.3:-70248095 None:intergenic
GAATTACTTGCTCCTAGTTC+TGG 0.266117 8.3:+70251204 MS.gene051115:CDS
AAGTCCTAACAATCTTAATT+TGG 0.267658 8.3:+70250782 MS.gene051115:CDS
CATTCCAAATTAAGATTGTT+AGG 0.277408 8.3:-70250786 None:intergenic
TACCCTGGCTTGTGGATTCT+TGG 0.281500 8.3:-70246859 None:intergenic
TCGTCTGACGAAGAGTATTT+AGG 0.285117 8.3:+70251024 MS.gene051115:CDS
GAAAAGAAAATTCTCTTTCC+AGG 0.286608 8.3:-70243891 None:intergenic
GATATGAAACTTGTATATTC+AGG 0.290338 8.3:-70250913 None:intergenic
TTGATTATTAGGCTTGGATT+TGG 0.309327 8.3:-70250652 None:intergenic
TCCTCAAATCTTTCATCTCT+TGG 0.311154 8.3:-70246626 None:intergenic
TCAGGATCTAATTTACTAAC+AGG 0.316657 8.3:-70248077 None:intergenic
GTTGCAAGCTACAAAATATA+TGG 0.317917 8.3:+70250975 MS.gene051115:CDS
TTGTCACTGTCGTTCAATTT+CGG 0.321906 8.3:-70250751 None:intergenic
TAATTACACAGCTGAATTTA+CGG 0.322203 8.3:+70243719 MS.gene051115:CDS
CCACTCACTTGATATAATCT+TGG 0.323901 8.3:-70246900 None:intergenic
AATGAATTCTCAGATGTTGA+TGG 0.326863 8.3:+70246749 MS.gene051115:CDS
GTCAAAGAAGACTAATATTA+AGG 0.327000 8.3:+70243686 MS.gene051115:CDS
ATCCTTCAACACTGCTTCAT+TGG 0.329544 8.3:-70243661 None:intergenic
GTATTAATCTTCTCATCAAC+GGG 0.348087 8.3:-70250876 None:intergenic
AATTTCGGACAGTATCCTTA+TGG 0.355580 8.3:+70250832 MS.gene051115:CDS
CATTTATATTGTCTGCTCAT+AGG 0.356521 8.3:+70249500 MS.gene051115:CDS
CTGTCATTGATTATTAGGCT+TGG 0.359024 8.3:-70250658 None:intergenic
GGATCCAATAACTTGAAAAT+TGG 0.359274 8.3:-70249541 None:intergenic
CAACATGCTGCACTTAATTT+CGG 0.362410 8.3:+70250817 MS.gene051115:CDS
CTATGAGCAGACAATATAAA+TGG 0.371741 8.3:-70249499 None:intergenic
GTAAGTCTCTCAATGGTAAC+AGG 0.380176 8.3:-70248044 None:intergenic
TTAGAAAATATAACAATTGA+AGG 0.381425 8.3:+70243807 MS.gene051115:CDS
AGAGGCAACAACCTTCGATT+TGG 0.381540 8.3:+70251049 MS.gene051115:CDS
GTGCTTCATAACTTGCTTCC+TGG 0.384287 8.3:+70246689 MS.gene051115:CDS
GATCTTGGAAACCCAGATAA+AGG 0.388829 8.3:+70245050 MS.gene051115:CDS
GGCCCTAACAAAAGAATTCA+TGG 0.391351 8.3:+70243100 MS.gene051115:CDS
CCATGGTTCGTCTTTCATAT+CGG 0.393755 8.3:-70250725 None:intergenic
GGTGAAGAAGAAGGAAATAT+TGG 0.394404 8.3:-70243143 None:intergenic
AGAATTCATGGGTTCTTCAT+TGG 0.395604 8.3:+70243112 MS.gene051115:CDS
TTGAGGAACCGTTGCGGTGC+TGG 0.401483 8.3:+70251168 MS.gene051115:CDS
TTGGTCAGCAATAGAAAATT+GGG 0.402009 8.3:+70250603 MS.gene051115:CDS
GAACTTAGCCTGCCTCCATC+TGG 0.403989 8.3:+70248941 MS.gene051115:CDS
TATGACACATACAATGATCT+TGG 0.412730 8.3:+70245035 MS.gene051115:CDS
CACTCACTTGATATAATCTT+GGG 0.413543 8.3:-70246899 None:intergenic
AGAAGACCATCTTCTGCATA+AGG 0.416449 8.3:-70250577 None:intergenic
TCTTGGGAATGTCATACTTA+AGG 0.423843 8.3:-70246883 None:intergenic
AAATTAAGATTGTTAGGACT+TGG 0.428810 8.3:-70250780 None:intergenic
TTTAGATGCAACTACTAATT+GGG 0.429267 8.3:+70249000 MS.gene051115:CDS
TGAGTAGTCCAACACTATAA+AGG 0.430450 8.3:-70246772 None:intergenic
TTAGACCATGTGGTTGTGTT+GGG 0.432526 8.3:-70250534 None:intergenic
GATCTTGAGGGAGGCTATCC+TGG 0.433367 8.3:-70249708 None:intergenic
TGATTATTAGGCTTGGATTT+GGG 0.433593 8.3:-70250651 None:intergenic
TGTTGGACTACTCATTAAGT+TGG 0.436827 8.3:+70246781 MS.gene051115:CDS
AGGCTAAGTTCAATTGCAAC+AGG 0.441630 8.3:-70248929 None:intergenic
TCTTTAAGTGCAGACTCTTG+TGG 0.444467 8.3:-70248110 None:intergenic
CAAGATCAATTCCTTTATCT+GGG 0.447942 8.3:-70245061 None:intergenic
ATTGAGTCTTGCTTCACTCC+TGG 0.448673 8.3:+70249631 MS.gene051115:CDS
GACCAATGAAGCAGTGTTGA+AGG 0.454038 8.3:+70243659 MS.gene051115:CDS
ATGGAAACAAAAGAGTTGTA+AGG 0.456748 8.3:+70243219 MS.gene051115:CDS
TTTGTAGCTTGCAACAAACT+AGG 0.458682 8.3:-70250967 None:intergenic
TTGGTGCACACCAGCATCAT+TGG 0.460866 8.3:-70249053 None:intergenic
TTGAGAAGGTGATTGATAGT+AGG 0.462008 8.3:+70251130 MS.gene051115:CDS
CAAGCTAGGAAAGATCTTCC+TGG 0.462757 8.3:+70243873 MS.gene051115:CDS
GAAAGACTTGATTTAAGGCC+AGG 0.464489 8.3:-70246707 None:intergenic
ACAATTGAAGGATTTGCTAC+TGG 0.466343 8.3:+70243819 MS.gene051115:CDS
AAATTAGATCCTGAAATCTA+TGG 0.468171 8.3:+70248086 MS.gene051115:CDS
GTGGCCTACATTGATTGATT+CGG 0.469250 8.3:-70250701 None:intergenic
AACTCCAGCTATTGCTTGAC+GGG 0.470233 8.3:-70248018 None:intergenic
TCTTCACTGATAGCTGCAAC+AGG 0.474972 8.3:-70243263 None:intergenic
GTTGGACTACTCATTAAGTT+GGG 0.477312 8.3:+70246782 MS.gene051115:CDS
ACCAAGAGATGAAAGATTTG+AGG 0.477798 8.3:+70246625 MS.gene051115:CDS
TTTAGACCATGTGGTTGTGT+TGG 0.478176 8.3:-70250535 None:intergenic
GCAATTCTTGGGTTCAAGCT+AGG 0.478592 8.3:+70243859 MS.gene051115:CDS
TTTGGAAAGGATCAATGCCT+TGG 0.478720 8.3:+70248844 MS.gene051115:CDS
GATCTTGCTAGACCAACTCT+TGG 0.479530 8.3:+70245077 MS.gene051115:CDS
GCTCTAACCTTAAATTTCAC+AGG 0.486545 8.3:-70243353 None:intergenic
CCATGTGGTTGTGTTGGGTC+TGG 0.492360 8.3:-70250529 None:intergenic
TCATCTCTTGGTATATACAT+AGG 0.492403 8.3:-70246614 None:intergenic
TAACTCCAGCTATTGCTTGA+CGG 0.493184 8.3:-70248019 None:intergenic
CGGCCAGTACGGCACCGTCT+AGG 0.495310 8.3:-70245122 None:intergenic
ATGAAGCAGTGTTGAAGGAT+TGG 0.497542 8.3:+70243664 MS.gene051115:CDS
TACCCTAGACGGTGCCGTAC+TGG 0.498027 8.3:+70245119 MS.gene051115:CDS
GAAGACCATCTTCTGCATAA+GGG 0.501628 8.3:-70250576 None:intergenic
TGCACCAACTTGCCCACCAT+TGG 0.505334 8.3:+70249068 MS.gene051115:CDS
CTAGGAGCAAGTAATTCATA+AGG 0.506030 8.3:-70251198 None:intergenic
CTCAAAAGTTTAATCCCAGC+AGG 0.506743 8.3:-70244942 None:intergenic
GGTATTAATCTTCTCATCAA+CGG 0.507218 8.3:-70250877 None:intergenic
CATGTTTGCTCCAATGATGC+TGG 0.508665 8.3:+70249043 MS.gene051115:CDS
GGCCGTGAACCATCTGATAC+AGG 0.510574 8.3:+70245140 MS.gene051115:CDS
TTTATCACAGTAATAGCTCC+TGG 0.510933 8.3:-70243762 None:intergenic
TACCTGTATCAGATGGTTCA+CGG 0.511896 8.3:-70245142 None:intergenic
CTGCTGCTTACAAAACCAAC+TGG 0.511913 8.3:+70249677 MS.gene051115:CDS
GATCCAAGAATCCACAAGCC+AGG 0.513956 8.3:+70246856 MS.gene051115:CDS
CTTGCTCCTAGTTCTGGACC+TGG 0.515196 8.3:+70251210 MS.gene051115:CDS
GTACGGCACCGTCTAGGGTA+TGG 0.515752 8.3:-70245116 None:intergenic
CTACCCGATGATACACCTGC+TGG 0.515885 8.3:+70244927 MS.gene051115:CDS
GGTGTATCATCGGGTAGATA+AGG 0.517991 8.3:-70244921 None:intergenic
TGAAAACATGGTGAAGAAGA+AGG 0.520459 8.3:-70243152 None:intergenic
GTTGATGGCCTTTATAGTGT+TGG 0.523396 8.3:+70246764 MS.gene051115:CDS
CTTAAGGATACCCTGGCTTG+TGG 0.525590 8.3:-70246867 None:intergenic
ATCTCCATGGCATAGCGACC+AGG 0.526081 8.3:-70249649 None:intergenic
GACCTGGTGTTACATGCAGA+GGG 0.527475 8.3:+70251226 MS.gene051115:CDS
ATATGAAACTTGTATATTCA+GGG 0.530940 8.3:-70250912 None:intergenic
TTTGCCCGTCAAGCAATAGC+TGG 0.532353 8.3:+70248014 MS.gene051115:CDS
CTTACCAATGGTGGGCAAGT+TGG 0.533372 8.3:-70249072 None:intergenic
ATCCCAGCAGGTGTATCATC+GGG 0.533446 8.3:-70244930 None:intergenic
CACTCCTGGTCGCTATGCCA+TGG 0.533642 8.3:+70249645 MS.gene051115:CDS
TGTTTGGTCCAGATGGAGGC+AGG 0.533655 8.3:-70248949 None:intergenic
ATTTCAGATCCTCCAAGAGT+TGG 0.535524 8.3:-70245089 None:intergenic
CTTGCTAGACCAACTCTTGG+AGG 0.537115 8.3:+70245080 MS.gene051115:CDS
CAACAACCTTCGATTTGGAC+AGG 0.537155 8.3:+70251054 MS.gene051115:CDS
TAATTATTACCTGTATCAGA+TGG 0.538174 8.3:-70245149 None:intergenic
GATTACCCTTATGCAGAAGA+TGG 0.539224 8.3:+70250571 MS.gene051115:CDS
GAAAGGATCAATGCCTTGGA+TGG 0.543205 8.3:+70248848 MS.gene051115:CDS
GTAACACCAGGTCCAGAACT+AGG 0.543782 8.3:-70251216 None:intergenic
TTGCTAACAATGTGAGGCAA+TGG 0.546196 8.3:-70246833 None:intergenic
TTGATAGTAGGAATGGTGAT+AGG 0.548139 8.3:+70251142 MS.gene051115:CDS
CCATTCTTGTGTTGAAAACA+TGG 0.549764 8.3:-70243164 None:intergenic
CTTGGTATATACATAGGAAG+TGG 0.550676 8.3:-70246608 None:intergenic
TCCACACATGAGGTACACTT+TGG 0.551392 8.3:+70249561 MS.gene051115:CDS
GGAATGGTGATAGGAATTTG+AGG 0.553948 8.3:+70251151 MS.gene051115:CDS
GGACCTGGTGTTACATGCAG+AGG 0.558911 8.3:+70251225 MS.gene051115:CDS
AATCCCAGCAGGTGTATCAT+CGG 0.559593 8.3:-70244931 None:intergenic
ACCTGCGGATGAGATCTTGA+GGG 0.562889 8.3:-70249720 None:intergenic
GGCCAGTACGGCACCGTCTA+GGG 0.564885 8.3:-70245121 None:intergenic
GATCGAGTGTTTGGTCCAGA+TGG 0.566962 8.3:-70248956 None:intergenic
TTAGTAGTTGCATCTAAAGC+AGG 0.568173 8.3:-70248995 None:intergenic
TTTGGCACGTAACCTCCATA+AGG 0.568931 8.3:-70250847 None:intergenic
TCCCTCAAGATCTCATCCGC+AGG 0.574537 8.3:+70249719 MS.gene051115:CDS
GGATCTAATTTACTAACAGG+TGG 0.574715 8.3:-70248074 None:intergenic
GTGCAGAGGATAAATTTGCT+TGG 0.574838 8.3:+70247976 MS.gene051115:intron
GATCTAATTTACTAACAGGT+GGG 0.574850 8.3:-70248073 None:intergenic
GCTACGCACACATGCTTGCA+TGG 0.575061 8.3:+70249474 MS.gene051115:CDS
AATCTGAGAGGTGATGGAAA+AGG 0.575229 8.3:+70244981 MS.gene051115:CDS
GAAAACTGTAAGTCTCTCAA+TGG 0.578316 8.3:-70248051 None:intergenic
ATCCTGGTCAAAACGCCAGT+TGG 0.579614 8.3:-70249692 None:intergenic
AAATGTATCCATACCCTAGA+CGG 0.586694 8.3:+70245108 MS.gene051115:CDS
ATCCAAGAATCCACAAGCCA+GGG 0.589581 8.3:+70246857 MS.gene051115:CDS
TTCGGACAGTATCCTTATGG+AGG 0.590508 8.3:+70250835 MS.gene051115:CDS
ATTATTAGCTTTAGACCATG+TGG 0.590586 8.3:-70250544 None:intergenic
TACCCGATGATACACCTGCT+GGG 0.591254 8.3:+70244928 MS.gene051115:CDS
TACCTGCGGATGAGATCTTG+AGG 0.594715 8.3:-70249721 None:intergenic
CAGATGGTTCACGGCCAGTA+CGG 0.595137 8.3:-70245133 None:intergenic
AGCAGCAGAACTAATCTCCA+TGG 0.597060 8.3:-70249662 None:intergenic
TGGATCTTGCTAACAATGTG+AGG 0.598996 8.3:-70246839 None:intergenic
GTATGCTTGCTTACCAATGG+TGG 0.599350 8.3:-70249081 None:intergenic
GCATCACCTGTCCAAATCGA+AGG 0.600030 8.3:-70251060 None:intergenic
AAGGTGATTGATAGTAGGAA+TGG 0.600577 8.3:+70251135 MS.gene051115:CDS
CAGAGCTTGATCAATGCAGA+TGG 0.602184 8.3:+70249604 MS.gene051115:CDS
TCGTTCAATTTCGGCCACCA+TGG 0.607867 8.3:-70250742 None:intergenic
TGTCATACTTAAGGATACCC+TGG 0.608790 8.3:-70246874 None:intergenic
TCCAAAGTGTACCTCATGTG+TGG 0.612282 8.3:-70249562 None:intergenic
CGAGTGTTTGGTCCAGATGG+AGG 0.614080 8.3:-70248953 None:intergenic
GCCCTAACAAAAGAATTCAT+GGG 0.614296 8.3:+70243101 MS.gene051115:CDS
TTTAAATGGTACCTCTTGGA+CGG 0.616838 8.3:-70248165 None:intergenic
ACCCCTCTGCATGTAACACC+AGG 0.617716 8.3:-70251228 None:intergenic
ATTAAGTGCAGCATGTTGCG+CGG 0.618193 8.3:-70250812 None:intergenic
ATGCAACTACTAATTGGGTG+TGG 0.623131 8.3:+70249005 MS.gene051115:CDS
ACGAAGAGTATTTAGGTGAG+AGG 0.628094 8.3:+70251031 MS.gene051115:CDS
ATGACAGAGAGTTACAAGCT+TGG 0.632533 8.3:+70250674 MS.gene051115:CDS
TACTCCGAATCAATCAATGT+AGG 0.641645 8.3:+70250697 MS.gene051115:CDS
GCAAAACACCAGCACCGCAA+CGG 0.642856 8.3:-70251176 None:intergenic
GCTTGAACCCAAGAATTGCA+AGG 0.647001 8.3:-70243855 None:intergenic
GGAAATTGGAAAGATTGAGA+AGG 0.652309 8.3:+70251116 MS.gene051115:CDS
AGGAATTTGAGGAACCGTTG+CGG 0.653263 8.3:+70251162 MS.gene051115:CDS
TATGCTTGCTTACCAATGGT+GGG 0.656904 8.3:-70249080 None:intergenic
TATTACTTGTGAGAGTCGTG+TGG 0.661476 8.3:+70246580 MS.gene051115:CDS
CCGATATGAAAGACGAACCA+TGG 0.663394 8.3:+70250725 MS.gene051115:CDS
TAATGGCATGACCGTCCAAG+AGG 0.670114 8.3:+70248154 MS.gene051115:CDS
TGCGGATGAGATCTTGAGGG+AGG 0.673318 8.3:-70249717 None:intergenic
AGTTATTGGATCCACACATG+AGG 0.685129 8.3:+70249551 MS.gene051115:CDS
GTTCGTCTTTCATATCGGCG+TGG 0.688334 8.3:-70250720 None:intergenic
CCAGACCCAACACAACCACA+TGG 0.693358 8.3:+70250529 MS.gene051115:CDS
ACCTGGTGTTACATGCAGAG+GGG 0.702111 8.3:+70251227 MS.gene051115:CDS
AGTAATAGTTCACATAAGTG+CGG 0.704279 8.3:-70250627 None:intergenic
TTAGAGCTGTTGTGACTGTG+AGG 0.719203 8.3:+70243369 MS.gene051115:CDS
ATTAATCTTCTCATCAACGG+GGG 0.722560 8.3:-70250874 None:intergenic
ATATGAAAGACGAACCATGG+TGG 0.746586 8.3:+70250728 MS.gene051115:CDS
ACAGGCTTTAGAGTACCAAG+TGG 0.757581 8.3:-70248911 None:intergenic
TATTAATCTTCTCATCAACG+GGG 0.765013 8.3:-70250875 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.3 gene 70243098 70251272 70243098 ID=MS.gene051115
chr8.3 mRNA 70243098 70251272 70243098 ID=MS.gene051115.t1;Parent=MS.gene051115
chr8.3 exon 70243098 70243503 70243098 ID=MS.gene051115.t1.exon1;Parent=MS.gene051115.t1
chr8.3 CDS 70243098 70243503 70243098 ID=cds.MS.gene051115.t1;Parent=MS.gene051115.t1
chr8.3 exon 70243640 70243920 70243640 ID=MS.gene051115.t1.exon2;Parent=MS.gene051115.t1
chr8.3 CDS 70243640 70243920 70243640 ID=cds.MS.gene051115.t1;Parent=MS.gene051115.t1
chr8.3 exon 70244921 70245161 70244921 ID=MS.gene051115.t1.exon3;Parent=MS.gene051115.t1
chr8.3 CDS 70244921 70245161 70244921 ID=cds.MS.gene051115.t1;Parent=MS.gene051115.t1
chr8.3 exon 70246579 70246914 70246579 ID=MS.gene051115.t1.exon4;Parent=MS.gene051115.t1
chr8.3 CDS 70246579 70246914 70246579 ID=cds.MS.gene051115.t1;Parent=MS.gene051115.t1
chr8.3 exon 70247982 70248175 70247982 ID=MS.gene051115.t1.exon5;Parent=MS.gene051115.t1
chr8.3 CDS 70247982 70248175 70247982 ID=cds.MS.gene051115.t1;Parent=MS.gene051115.t1
chr8.3 exon 70248788 70249089 70248788 ID=MS.gene051115.t1.exon6;Parent=MS.gene051115.t1
chr8.3 CDS 70248788 70249089 70248788 ID=cds.MS.gene051115.t1;Parent=MS.gene051115.t1
chr8.3 exon 70249474 70249740 70249474 ID=MS.gene051115.t1.exon7;Parent=MS.gene051115.t1
chr8.3 CDS 70249474 70249740 70249474 ID=cds.MS.gene051115.t1;Parent=MS.gene051115.t1
chr8.3 exon 70250516 70251272 70250516 ID=MS.gene051115.t1.exon8;Parent=MS.gene051115.t1
chr8.3 CDS 70250516 70251272 70250516 ID=cds.MS.gene051115.t1;Parent=MS.gene051115.t1
Gene Sequence

>MS.gene051115

ATGGCCCTAACAAAAGAATTCATGGGTTCTTCATTGGTAGAGAGACCAATATTTCCTTCTTCTTCACCATGTTTTCAACACAAGAATGGTGGATTCTTGATCAGTCCTGTTTTTGTTCCTTATGGAAACAAAAGAGTTGTAAGGTTGAGAAAATCTGCAAAGTTTCCTGTTGCAGCTATCAGTGAAGATTTGATGAAAAGCTCTTCATCATCTTCTTCTATATCATCATCTTCTTCTTCTGTTCCTGCTGAAAAACCTGTGAAATTTAAGGTTAGAGCTGTTGTGACTGTGAGGAACAAGATCAAAGAAGATTTCAAAGAGACTTTTGTGAAACATCTTGATGCTTTTACTGATAGGATTGGAAGAAATGTTGTTCTTGAACTTTTTAGCACTGAGATTGATCCAAGTAAGACTTTTCTTCATTGTGTTAATTAAAAATCAATATTTACTTTATACACTTAATATACAAACATACACTTAATTTTAATTTCTTTGATTTTGATAATTAAATTATTGGTGATTTTTATGTTGTGATCTTTCAGAAACAAAAGCTGCAAAGAAGACCAATGAAGCAGTGTTGAAGGATTGGTCAAAGAAGACTAATATTAAGGCAGAGAGAGTTAATTACACAGCTGAATTTACGGTTGACTCAAATTTTGGAGAACCAGGAGCTATTACTGTGATAAATAATCATCAGCAAGAATTTTACTTAGAAAATATAACAATTGAAGGATTTGCTACTGGAGCATTTCATTTTCCTTGCAATTCTTGGGTTCAAGCTAGGAAAGATCTTCCTGGAAAGAGAATTTTCTTTTCTAATAAGGTATCTAATAATTAATTCTTCAACTCTTTTTTTGTTATTTATCTGTCATCATAACATGGCCCAGTAAGAAAAAAAAAAGTAGTACAGCCAAAAAAGTAGTATCGTAAGGCAACAATATTTGTTTATTCATTTTTTCAACGTGAGAAATAAGGTGAAAATTGAAAGTTTTTTTTTTGAAATAAGGTGAAAATTGAAAGTTGAAAGGTATAATAATTTTTGTTTTGAAGAATCTTGAAAAGGCACAATAATTCCAAAAGTTCATTTTAACGTAAAAATATTGAATGTTATTTAATAATTATTTTATGTGCCAAAACCAAGCAGTCCGTGATTTTATAATTATTGTTATATATCATTGCATTTTTTAATTAATTATCCACGATAAAGTGATATACTAATAAACAATTAACCTAGAAAATCATATAGTCAAATTTATGCATATGGACCAAGCACCACTGAATTGACCTGACGACTACCTACGGTATGAATTGGGTAAAGACACCGTTGAATTGAATACTAGTAGTACTTGTCAAGAAAAAAAATAATACTAGCAAATACCCTCTTTATCTTTATTTTTCGAGTCAGCAACATTCTTTAACACTCACTCGTTTATTAGATTAAATTAAAGTGGGTCTCGCACGTTGAAAATTGATTTTCTTAAAGTGATGAAATATATATTGATTCCATCTAACAAGAGAGTGAGTATTAGAAACTATTAAAATGAAAGTATCTCTCGCATTTCTCTTATTTTTCTGACTAGTACTAGTAGTCTTTATGGTCGAGGATGGATGTGGAACAATAATCTTACCCTTGGCATTATTCAGCATTTACCTAATGTAAATTTATACAAATAATAAAGTGGTAACATTCCCTAAATAAAAAAATAAAGTGGTAACATTTACCTTTCTCGATCTTTTGACTAAGATCAAGTGTAGTATAAATATTCTTTTCGAAATAAAGTGGTAACATATGTTTGATTTAATGTGCGATTTACTTTGAACAGCCTTATCTACCCGATGATACACCTGCTGGGATTAAACTTTTGAGAGAGAAAGACCTGAAAAATCTGAGAGGTGATGGAAAAGGAGTTAGAAAATTATCTGACAGAATATATGATTATGACACATACAATGATCTTGGAAACCCAGATAAAGGAATTGATCTTGCTAGACCAACTCTTGGAGGATCTGAAATGTATCCATACCCTAGACGGTGCCGTACTGGCCGTGAACCATCTGATACAGGTAATAATTAATTATATAAGCAATTTTACACTACTAATGATTAATGAATATTGCATTGTATCCTTTAGTAGGGTGTTAAACTATATTTCTATCCTTTAGGGTGTGTTTGGTTAAGAAGAGAAGTTGTGAAGAGAGAAATAAGTATGAGAGAGTATGAGAAGAGAGAAAAAGGTGAGAAATAGAATAGATTTGATGTAGTGTTTGGTATAAAAGAAAGAGAAGAGAAACAGAGAAGAGAGAAGTGTTGATTGTTAATAAAAGTACAAAAATAACCTTGATTTTAAAAAAGTTCCTTCACAATAATTTTTTATTAGCTATTTTTATTTTTATTTATTTGTAGGCCTTATTTGAATTATAACTATTTTAGATAATTAAGTTAACTATTGATTTAGTTATTAAATTTTTTATTTTAATCACGTGGTGGGTACATGATGTTGAAGTCTGGAAACAAAAACTACATAACAATGTGTGTGAAACACATGAAACAAAAGGGCAATATTGGAAAATCAAAAATTAAATGGTGCTCTCTCCACTTTCTTTGCTACCTTGGAGAGAAGAAAAAAACATGTGGGACCCAGTCATATTTATTCTCTCTTCGATACTTCTTTGATATGCCAAACGTGAACAGTAACCATTTTCTTTGCATTCTCTCTCGTGCCTTTCTCTCTCGTCCCAACTTCTCGCGTAACAAACACACTGTTAAAGTGCGATGTTAGATGATTCGGGTTTGAACTTCGACTCATTCATTTGTATGTATAAGTAAATTTCCAACGACTATTGTGATTTCATCTATCTAAAAAAAAGTCTTGTTAGAATACTATATGAAACTAAGAAGTTGACAAAAACATAATTTCTCTTTATGAAATTATATGTTGTCCTCGTTTTAGTTGCACATTGTATTCTAAGTTGTATTTAAAGTTATATCTTGTGGTCTTGTAAATGCAATTCAGATGATAAAATTCTAAAATGTTACAATGACATTTGATATGATGGTGTGTGGGCTTAAACTCGCATTTCTCCACTTCTCTATACTTAGTGTGTGTCTATGTACGTGTGAATTTCGCTGCAAATCTCCCTTGGAAAAAAAGTGTGATAGCTTGGAAATATAAAAAATTATTTTACATTCAAATTTAGTAAATTTTTACAAATCAATTTGATTTTAAGGATTAAAATTTATACTAATTTGATGTATTTTCTAACATAGGATCTTCTCTGGACCAACTTGTGGGCACCGACATCTTGTGATGCTTTACAGTGGTGTCATGGCATGCTATAATATTATTTTCTTAAATATTTGTACAAATATTTACTTGATAAAATATGATTATCTGTTTAAAATTATGTAGATTATAATATGTTACTATGCTTTGTGGTGATTCTAAATAACTAAACAAAATTGAATTGGATGGTGCTGCAGATATTACTTGTGAGAGTCGTGTGGAGAAGCCACTTCCTATGTATATACCAAGAGATGAAAGATTTGAGGAATCCAAAATGAACACATTTTCAGTGAAGAGGCTAAAAGGAGTGCTTCATAACTTGCTTCCTGGCCTTAAATCAAGTCTTTCTGCTCAAAACAGAGATTTCAATGAATTCTCAGATGTTGATGGCCTTTATAGTGTTGGACTACTCATTAAGTTGGGTTTGCAAGATGATATTTTGAAGAAACTTCCATTGCCTCACATTGTTAGCAAGATCCAAGAATCCACAAGCCAGGGTATCCTTAAGTATGACATTCCCAAGATTATATCAAGTGAGTGGAATATGCACTTGTTATTGTCGATTTCTTGATTTGCTTGCTAAATAAATTTGGTACATAATTGAGTTAAGCTAATTCTTTTCATAAGTAAATCTTTGGTTTAAATACACTTTTAGTCCTCTCGTCCGATTCACAATTTTGATTCTCCATTTTTTAAATTTTTTGAAAATTTCCGCATTACTGAATTTTGGGCTTAATTTAGCAGTGTGTTAGGTTGAAGAATGAATTAACAAGTCATTGGATGGAAAATGTAGCATTGTATATTTTTTGAGATATAATTTTCTTAGGGTTTTACACTTTCGTCATTTAGAAAATTTCCTCTTTGTATAAAATTTCTAAACTTAATATTTCCAAATTGAATTATGTCTTTATAAAAAATTTAACTTAGATTTGAAATACCTTTAAATCATATACCAATTACATTAAAGGAATATTTATACTTTTTTTACTTTCCCTTTCATTTGCAATGTGGTGGGGTCTTTGGCCATGAATATTCTATAATGGAATGCAAGCATGCCATATGAAAAGATAGTGCTACAAATTAAAGAGTGCCACAGTACAACATGTTATTTGGCTTTTAGTGTTTGTCAGTACAATTTCAACCCAAAAAGATGAGTTTTCAGTTCAAAGCTAGTAGATCTAATGTTTATACCATTCCTTTTATATATAAAAAAATTACAACTGGGCCAATTTGTTATTAATTATTACTTGTTCATTTGAAACTAAACTGTCACCAAAGCTGTACTAATCAATTGTTAATTTTAAATGTTTCACATGTGTGTCTAGTTATTATAAACTGTTGATATAAAAATTAAGGAAAATGCTAACTTGTGCCCTCAAGGCACAAGTTAAGAAACCAAAGATAGAAACATTTTGTTGGAAATTGTGCATTCAATATTATAAAAATTTAAAATCTTTTCTTTTCAAGACATAATTTATGATTGAATTCCATTTTTAGATTCTTAACTTGTGTCCTAAGGGTACAAGTTAACACTACCCAAAAATTAAAGTTCTTGTTTAATTAATTGTGAAATTATTGATCTCATGTGCCTAATGTGCAGAGGATAAATTTGCTTGGTTGAGAGATGATGAATTTGCCCGTCAAGCAATAGCTGGAGTTAATCCTGTTACCATTGAGAGACTTACAGTTTTCCCACCTGTTAGTAAATTAGATCCTGAAATCTATGGTCCACAAGAGTCTGCACTTAAAGAAGAGCACATTTTAAATCAACTTAATGGCATGACCGTCCAAGAGGTACCATTTAAAGCATTCAAAATTTTTTTCCTTTTTGGTACAATGCATTCATAAACTTACTAACCATGTTACCTAATCAAGTGCCTTGTGAATTGTTTTTTCAAACTATTTAACCAATTATAACATTTCCCATGGATTAATTTTTTCACTTGACATCAAATTTATAATGGTCACCAAGTGGTTTGGTTCAAGTAGTGAGTGATTTGGATACTTTAAACAAATGGTCAGAGATTTGACTACTCAGGATCGACCTAAAGACTAAGCAATTTAAGTTAGGGTTTTAAGTCTCACTTCAAAATTTGTTTTAGACTTCTAAACATATTGTGTACGTCCTGAGGCAACTAACTCTTGTATATGGAGAAAAATCAGTTAAAAATGGCGAATCTATCATGGTGCCCAATAGGTTCCAACAAAAATTACCACTGTTAAAGGACAATGATACTTCGTGCCTATAACTTGATTTTAAAGAAATAAATTTAAAATTATCCTATGTATGCTCTTGAATGCACAATTCAATACTAGTAGATCATATAAATTTTGACATTTTTTTTTTATTAATTTACTTATTTGCACTAAACTCCCACTTTCTTTTCTTTTTTTGGAATTTTGCAGGCAATAGATCAAAATAAGTTGTTTATAATTGATTATCATGATATCTACCTTCCATTTTTGGAAAGGATCAATGCCTTGGATGGTAGAAAATCATATGCCACACGTACCATATACTTTTTGACACCACTTGGTACTCTAAAGCCTGTTGCAATTGAACTTAGCCTGCCTCCATCTGGACCAAACACTCGATCAAAACGTGTAGTTACACCTGCTTTAGATGCAACTACTAATTGGGTGTGGATGCTTGCAAAAGCTCATGTTTGCTCCAATGATGCTGGTGTGCACCAACTTGCCCACCATTGGTAAGCAAGCATACAATTTTTCATTTACTTAGTCAATCATCAATTTAGTCCTAAAATTTTATGTGACAAGTGACACAAGACATTTACACTCACACATGGACACTGATAATAATTTAATGAAATAATTGAATATAATCACATATGTTGTATCAAATTGACATGTGTCGGACACAAAATATGTCTTTCATGTGAAAAGACATTGCGACATAGGTTTGAAAAAATTGTTTAGAAATTATTTTGCAATTTTGTTAGTTTCATTGACACAATACCTTGACACATGCAACTTAAATACTAAATTGGTGATTGAAGTACTAGAACTTTTTTTTATATAACTGGCATGAACTTATTTCTTTGCTTATTTTTTCTCGGTGAATTTTTATATAGGCTACGCACACATGCTTGCATGGAACCATTTATATTGTCTGCTCATAGGCAATTAAGTGCAATGCATCCAATTTTCAAGTTATTGGATCCACACATGAGGTACACTTTGGAGATCAATGCTTTAGCTCGACAGAGCTTGATCAATGCAGATGGTGTCATTGAGTCTTGCTTCACTCCTGGTCGCTATGCCATGGAGATTAGTTCTGCTGCTTACAAAACCAACTGGCGTTTTGACCAGGATAGCCTCCCTCAAGATCTCATCCGCAGGTAAATATCATATATTAATTTCGGCATTTTTTTCCGCTTTGTTTTAAAGATCCTAGAACGAAACTCACACACGCAATACGGAGAATAGACATATTTTTTGCTTCTAACCTCTGTACATCAATTGTTTTCGCTGCCTTTTACCATTTGAGGCGCATTGTTGGTTCGTTATAACTTATTAGTTTATGACCTCAACTCTAAAGTTTAAAATAAGGGTCTGTGATTGTGGTCTGTGATTGTATCAGCTTCGTTTAACTACGATTCTTTGCAATATTAAGGATCGTGACATGACCACTCTCTAAAACCCTCCTCAACTATATTTTGATACCCAAGAGAAATTGTAAATATTAAACTTTCTTTTATGATTTGTTTGAGGCGTCACTTAGTATATGTTTGATTTCACTTTAGTCAAAATTGATTCTAGAAGTGTATAATTGTTTTTTATATGTTTGAGTGCATTTCGGAATAATTGATTTTGACTCTAGAATTGATTTTAATTTGAAACTATGATTTATAGTTTTTTGACTCTAAACGTTAATTTTACACTTAAATTTATTGTTCAATCCACTATATGTGAATTTGTCCAAACGTAAACCATTTTACATTCAACTTAGTTTTAACTAAAATCAATTTTTCTCACCATAAAATCAAATACACACCTAGCTTCTACGGAAAAGAGACCCTACACTTTTCTTAACATGTAATTGATGTTTGCTTTATTTGGTGCAAGCATATGACATATAAATCTAACTAAGATTTTCACTTTTTAACATTTCAGGGGAATGGCAGTACCAGACCCAACACAACCACATGGTCTAAAGCTAATAATGAAAGATTACCCTTATGCAGAAGATGGTCTTCTAATTTGGTCAGCAATAGAAAATTGGGTCCGCACTTATGTGAACTATTACTACCCAAATCCAAGCCTAATAATCAATGACAGAGAGTTACAAGCTTGGTACTCCGAATCAATCAATGTAGGCCACGCCGATATGAAAGACGAACCATGGTGGCCGAAATTGAACGACAGTGACAATCTTGTCCAAGTCCTAACAATCTTAATTTGGAATGCATCCGCGCAACATGCTGCACTTAATTTCGGACAGTATCCTTATGGAGGTTACGTGCCAAATCGTCCCCCGTTGATGAGAAGATTAATACCAGAAGAAAATGACCCTGAATATACAAGTTTCATATCTGATCCACAAAAGTATTTTCTCAATGCATTGCCTAGTTTGTTGCAAGCTACAAAATATATGGCTGTTGTTGACACGCTTTCGACTCATTCGTCTGACGAAGAGTATTTAGGTGAGAGGCAACAACCTTCGATTTGGACAGGTGATGCTGAGATTGTCGAAGCATTTTATCAATTCTCTGCGGAAATTGGAAAGATTGAGAAGGTGATTGATAGTAGGAATGGTGATAGGAATTTGAGGAACCGTTGCGGTGCTGGTGTTTTGCCTTATGAATTACTTGCTCCTAGTTCTGGACCTGGTGTTACATGCAGAGGGGTTCCAAATAGTGTGTCCACATAA
Protein sequence

>MS.gene051115.t1

MALTKEFMGSSLVERPIFPSSSPCFQHKNGGFLISPVFVPYGNKRVVRLRKSAKFPVAAISEDLMKSSSSSSSISSSSSSVPAEKPVKFKVRAVVTVRNKIKEDFKETFVKHLDAFTDRIGRNVVLELFSTEIDPKTKAAKKTNEAVLKDWSKKTNIKAERVNYTAEFTVDSNFGEPGAITVINNHQQEFYLENITIEGFATGAFHFPCNSWVQARKDLPGKRIFFSNKPYLPDDTPAGIKLLREKDLKNLRGDGKGVRKLSDRIYDYDTYNDLGNPDKGIDLARPTLGGSEMYPYPRRCRTGREPSDTDITCESRVEKPLPMYIPRDERFEESKMNTFSVKRLKGVLHNLLPGLKSSLSAQNRDFNEFSDVDGLYSVGLLIKLGLQDDILKKLPLPHIVSKIQESTSQGILKYDIPKIISKDKFAWLRDDEFARQAIAGVNPVTIERLTVFPPVSKLDPEIYGPQESALKEEHILNQLNGMTVQEAIDQNKLFIIDYHDIYLPFLERINALDGRKSYATRTIYFLTPLGTLKPVAIELSLPPSGPNTRSKRVVTPALDATTNWVWMLAKAHVCSNDAGVHQLAHHWLRTHACMEPFILSAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLINADGVIESCFTPGRYAMEISSAAYKTNWRFDQDSLPQDLIRRGMAVPDPTQPHGLKLIMKDYPYAEDGLLIWSAIENWVRTYVNYYYPNPSLIINDRELQAWYSESINVGHADMKDEPWWPKLNDSDNLVQVLTILIWNASAQHAALNFGQYPYGGYVPNRPPLMRRLIPEENDPEYTSFISDPQKYFLNALPSLLQATKYMAVVDTLSTHSSDEEYLGERQQPSIWTGDAEIVEAFYQFSAEIGKIEKVIDSRNGDRNLRNRCGAGVLPYELLAPSSGPGVTCRGVPNSVST