Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25136.t1 | XP_003627280.1 | 99.5 | 209 | 1 | 0 | 1 | 209 | 1 | 209 | 1.10E-111 | 412.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25136.t1 | Q9ZRA4 | 58.9 | 207 | 82 | 3 | 3 | 207 | 2 | 207 | 1.7e-60 | 233.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25136.t1 | G7L6V2 | 99.5 | 209 | 1 | 0 | 1 | 209 | 1 | 209 | 8.2e-112 | 412.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05726 | MS.gene25136 | 0.822152 | 2.76E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25136.t1 | MTR_8g020670 | 99.522 | 209 | 1 | 0 | 1 | 209 | 1 | 209 | 7.49e-152 | 419 |
| MS.gene25136.t1 | MTR_8g020630 | 88.038 | 209 | 25 | 0 | 1 | 209 | 1 | 209 | 6.63e-134 | 374 |
| MS.gene25136.t1 | MTR_8g020650 | 87.560 | 209 | 26 | 0 | 1 | 209 | 1 | 209 | 1.08e-126 | 356 |
| MS.gene25136.t1 | MTR_2g044040 | 72.249 | 209 | 58 | 0 | 1 | 209 | 1 | 209 | 4.96e-106 | 303 |
| MS.gene25136.t1 | MTR_2g045100 | 70.813 | 209 | 61 | 0 | 1 | 209 | 1 | 209 | 6.78e-104 | 298 |
| MS.gene25136.t1 | MTR_2g036120 | 70.048 | 207 | 62 | 0 | 1 | 207 | 1 | 207 | 6.37e-101 | 291 |
| MS.gene25136.t1 | MTR_3g019710 | 67.464 | 209 | 68 | 0 | 1 | 209 | 1 | 209 | 1.83e-98 | 285 |
| MS.gene25136.t1 | MTR_0284s0010 | 67.464 | 209 | 68 | 0 | 1 | 209 | 1 | 209 | 2.36e-98 | 284 |
| MS.gene25136.t1 | MTR_3g019650 | 66.986 | 209 | 68 | 1 | 1 | 209 | 1 | 208 | 1.73e-95 | 277 |
| MS.gene25136.t1 | MTR_3g019680 | 66.986 | 209 | 68 | 1 | 1 | 209 | 1 | 208 | 6.10e-95 | 275 |
| MS.gene25136.t1 | MTR_5g031460 | 65.072 | 209 | 73 | 0 | 1 | 209 | 1 | 209 | 1.25e-93 | 272 |
| MS.gene25136.t1 | MTR_7g011340 | 66.986 | 209 | 69 | 0 | 1 | 209 | 1 | 209 | 5.02e-90 | 263 |
| MS.gene25136.t1 | MTR_4g010340 | 61.836 | 207 | 76 | 2 | 3 | 209 | 2 | 205 | 1.71e-85 | 251 |
| MS.gene25136.t1 | MTR_3g019810 | 62.069 | 203 | 71 | 1 | 1 | 203 | 107 | 303 | 3.60e-79 | 239 |
| MS.gene25136.t1 | MTR_8g020590 | 57.767 | 206 | 84 | 3 | 6 | 209 | 4 | 208 | 5.18e-79 | 235 |
| MS.gene25136.t1 | MTR_2g041550 | 55.502 | 209 | 89 | 3 | 3 | 209 | 4 | 210 | 1.81e-76 | 229 |
| MS.gene25136.t1 | MTR_2g041580 | 55.024 | 209 | 90 | 3 | 3 | 209 | 4 | 210 | 2.87e-76 | 228 |
| MS.gene25136.t1 | MTR_8g020610 | 56.311 | 206 | 86 | 3 | 6 | 209 | 4 | 207 | 3.31e-76 | 228 |
| MS.gene25136.t1 | MTR_2g041570 | 54.545 | 209 | 91 | 3 | 3 | 209 | 4 | 210 | 2.31e-75 | 226 |
| MS.gene25136.t1 | MTR_2g019780 | 53.488 | 215 | 91 | 3 | 1 | 209 | 1 | 212 | 3.17e-75 | 226 |
| MS.gene25136.t1 | MTR_3g073170 | 56.522 | 207 | 84 | 3 | 6 | 209 | 7 | 210 | 1.04e-74 | 224 |
| MS.gene25136.t1 | MTR_8g020760 | 54.976 | 211 | 93 | 2 | 1 | 209 | 1 | 211 | 6.90e-74 | 222 |
| MS.gene25136.t1 | MTR_8g020770 | 50.239 | 209 | 102 | 2 | 3 | 209 | 4 | 212 | 2.59e-69 | 211 |
| MS.gene25136.t1 | MTR_8g020763 | 53.846 | 182 | 83 | 1 | 29 | 209 | 9 | 190 | 3.69e-66 | 202 |
| MS.gene25136.t1 | MTR_8g020750 | 48.804 | 209 | 105 | 2 | 3 | 209 | 4 | 212 | 1.43e-63 | 196 |
| MS.gene25136.t1 | MTR_4g027415 | 66.901 | 142 | 47 | 0 | 1 | 142 | 1 | 142 | 1.63e-55 | 173 |
| MS.gene25136.t1 | MTR_2g072560 | 66.957 | 115 | 38 | 0 | 1 | 115 | 1 | 115 | 6.92e-50 | 159 |
| MS.gene25136.t1 | MTR_3g019610 | 65.094 | 106 | 37 | 0 | 1 | 106 | 1 | 106 | 9.26e-44 | 142 |
| MS.gene25136.t1 | MTR_7g111240 | 37.500 | 192 | 116 | 3 | 21 | 208 | 23 | 214 | 1.13e-36 | 127 |
| MS.gene25136.t1 | MTR_7g111240 | 37.500 | 192 | 113 | 4 | 21 | 208 | 23 | 211 | 1.15e-35 | 125 |
| MS.gene25136.t1 | MTR_1g085140 | 35.602 | 191 | 119 | 3 | 22 | 208 | 28 | 218 | 2.33e-35 | 124 |
| MS.gene25136.t1 | MTR_1g085500 | 33.854 | 192 | 123 | 3 | 21 | 208 | 26 | 217 | 2.43e-33 | 119 |
| MS.gene25136.t1 | MTR_2g031270 | 35.079 | 191 | 115 | 4 | 21 | 205 | 26 | 213 | 1.86e-31 | 114 |
| MS.gene25136.t1 | MTR_1g079490 | 34.634 | 205 | 122 | 4 | 13 | 205 | 13 | 217 | 2.61e-31 | 114 |
| MS.gene25136.t1 | MTR_1g112200 | 33.990 | 203 | 125 | 5 | 6 | 205 | 11 | 207 | 4.15e-31 | 113 |
| MS.gene25136.t1 | MTR_6g005310 | 36.916 | 214 | 122 | 6 | 4 | 205 | 3 | 215 | 5.21e-31 | 113 |
| MS.gene25136.t1 | MTR_6g005330 | 38.220 | 191 | 110 | 4 | 22 | 205 | 26 | 215 | 6.24e-31 | 113 |
| MS.gene25136.t1 | MTR_2g030855 | 35.079 | 191 | 115 | 4 | 21 | 205 | 25 | 212 | 1.17e-30 | 112 |
| MS.gene25136.t1 | MTR_6g005360 | 38.220 | 191 | 110 | 4 | 22 | 205 | 26 | 215 | 1.25e-30 | 112 |
| MS.gene25136.t1 | MTR_6g005340 | 36.449 | 214 | 123 | 6 | 4 | 205 | 3 | 215 | 2.16e-30 | 111 |
| MS.gene25136.t1 | MTR_4g017040 | 35.023 | 217 | 126 | 6 | 1 | 204 | 1 | 215 | 2.33e-30 | 111 |
| MS.gene25136.t1 | MTR_1g060120 | 33.019 | 212 | 130 | 4 | 6 | 205 | 10 | 221 | 2.55e-30 | 111 |
| MS.gene25136.t1 | MTR_4g017050 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 3.28e-30 | 111 |
| MS.gene25136.t1 | MTR_5g046410 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 4.87e-30 | 110 |
| MS.gene25136.t1 | MTR_5g046430 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 4.87e-30 | 110 |
| MS.gene25136.t1 | MTR_4g017030 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 4.87e-30 | 110 |
| MS.gene25136.t1 | MTR_2g019250 | 34.562 | 217 | 127 | 6 | 1 | 204 | 1 | 215 | 6.85e-30 | 110 |
| MS.gene25136.t1 | MTR_1g023770 | 32.367 | 207 | 128 | 4 | 5 | 205 | 9 | 209 | 1.90e-29 | 108 |
| MS.gene25136.t1 | MTR_6g005380 | 36.500 | 200 | 112 | 5 | 9 | 194 | 7 | 205 | 1.38e-27 | 104 |
| MS.gene25136.t1 | MTR_5g036570 | 31.937 | 191 | 125 | 4 | 22 | 207 | 31 | 221 | 1.82e-27 | 104 |
| MS.gene25136.t1 | MTR_2g030895 | 34.940 | 166 | 101 | 4 | 47 | 205 | 13 | 178 | 4.42e-26 | 99.4 |
| MS.gene25136.t1 | MTR_2g086630 | 37.129 | 202 | 116 | 6 | 9 | 205 | 9 | 204 | 1.45e-25 | 99.0 |
| MS.gene25136.t1 | MTR_6g005350 | 33.491 | 212 | 128 | 5 | 6 | 205 | 4 | 214 | 2.77e-25 | 98.6 |
| MS.gene25136.t1 | MTR_2g086620 | 36.082 | 194 | 115 | 5 | 17 | 205 | 15 | 204 | 9.02e-25 | 96.7 |
| MS.gene25136.t1 | MTR_2g030865 | 34.940 | 166 | 101 | 4 | 47 | 205 | 13 | 178 | 1.43e-24 | 95.5 |
| MS.gene25136.t1 | MTR_8g007300 | 36.090 | 133 | 84 | 1 | 38 | 169 | 48 | 180 | 1.83e-24 | 96.3 |
| MS.gene25136.t1 | MTR_3g096330 | 34.211 | 152 | 97 | 2 | 21 | 169 | 580 | 731 | 5.23e-23 | 96.7 |
| MS.gene25136.t1 | MTR_2g086640 | 39.695 | 131 | 77 | 2 | 77 | 205 | 73 | 203 | 2.12e-22 | 90.5 |
| MS.gene25136.t1 | MTR_2g031300 | 42.000 | 100 | 54 | 2 | 47 | 142 | 13 | 112 | 1.60e-19 | 81.6 |
| MS.gene25136.t1 | MTR_2g096110 | 37.324 | 142 | 72 | 3 | 40 | 178 | 2 | 129 | 4.15e-19 | 80.1 |
| MS.gene25136.t1 | MTR_4g052780 | 35.556 | 135 | 85 | 2 | 73 | 205 | 71 | 205 | 4.82e-18 | 79.0 |
| MS.gene25136.t1 | MTR_4g052770 | 34.815 | 135 | 86 | 2 | 73 | 205 | 71 | 205 | 1.36e-17 | 77.8 |
| MS.gene25136.t1 | MTR_7g029500 | 56.604 | 53 | 23 | 0 | 150 | 202 | 38 | 90 | 5.22e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25136.t1 | AT1G72610 | 58.854 | 192 | 77 | 2 | 20 | 209 | 17 | 208 | 2.49e-76 | 228 |
| MS.gene25136.t1 | AT5G20630 | 54.545 | 209 | 93 | 2 | 1 | 207 | 1 | 209 | 3.43e-75 | 226 |
| MS.gene25136.t1 | AT5G39190 | 37.557 | 221 | 122 | 6 | 1 | 206 | 1 | 220 | 4.85e-40 | 136 |
| MS.gene25136.t1 | AT5G39160 | 38.140 | 215 | 117 | 6 | 3 | 206 | 7 | 216 | 1.10e-39 | 135 |
| MS.gene25136.t1 | AT5G39110 | 38.636 | 220 | 120 | 5 | 1 | 205 | 1 | 220 | 1.21e-39 | 135 |
| MS.gene25136.t1 | AT5G39160 | 38.140 | 215 | 121 | 6 | 3 | 206 | 7 | 220 | 1.60e-39 | 135 |
| MS.gene25136.t1 | AT5G39130 | 37.104 | 221 | 123 | 6 | 1 | 206 | 1 | 220 | 5.85e-39 | 134 |
| MS.gene25136.t1 | AT3G04200 | 38.679 | 212 | 110 | 7 | 12 | 206 | 15 | 223 | 3.25e-38 | 132 |
| MS.gene25136.t1 | AT3G62020 | 36.967 | 211 | 123 | 6 | 8 | 208 | 8 | 218 | 3.28e-38 | 132 |
| MS.gene25136.t1 | AT1G09560 | 38.889 | 198 | 117 | 3 | 12 | 205 | 18 | 215 | 4.00e-38 | 131 |
| MS.gene25136.t1 | AT3G05930 | 36.493 | 211 | 126 | 4 | 3 | 205 | 5 | 215 | 5.90e-38 | 131 |
| MS.gene25136.t1 | AT3G05950 | 39.713 | 209 | 110 | 6 | 12 | 205 | 14 | 221 | 8.03e-38 | 131 |
| MS.gene25136.t1 | AT1G18980 | 36.765 | 204 | 123 | 4 | 6 | 205 | 17 | 218 | 9.42e-38 | 130 |
| MS.gene25136.t1 | AT5G39180 | 37.900 | 219 | 122 | 5 | 1 | 205 | 1 | 219 | 4.91e-37 | 129 |
| MS.gene25136.t1 | AT5G39150 | 37.900 | 219 | 122 | 5 | 1 | 205 | 1 | 219 | 1.91e-36 | 127 |
| MS.gene25136.t1 | AT1G02335 | 35.961 | 203 | 124 | 4 | 12 | 208 | 16 | 218 | 2.42e-36 | 127 |
| MS.gene25136.t1 | AT5G39120 | 38.356 | 219 | 121 | 6 | 1 | 205 | 1 | 219 | 4.41e-36 | 126 |
| MS.gene25136.t1 | AT5G26700 | 35.122 | 205 | 130 | 3 | 6 | 208 | 9 | 212 | 5.55e-36 | 125 |
| MS.gene25136.t1 | AT5G39190 | 35.849 | 212 | 116 | 4 | 1 | 206 | 1 | 198 | 7.88e-36 | 125 |
| MS.gene25136.t1 | AT3G62020 | 38.068 | 176 | 104 | 4 | 38 | 208 | 14 | 189 | 1.77e-35 | 124 |
| MS.gene25136.t1 | AT5G39160 | 35.377 | 212 | 117 | 4 | 1 | 206 | 1 | 198 | 2.49e-35 | 124 |
| MS.gene25136.t1 | AT5G38960 | 36.548 | 197 | 114 | 5 | 20 | 206 | 28 | 223 | 3.96e-34 | 121 |
| MS.gene25136.t1 | AT1G18970 | 35.323 | 201 | 124 | 4 | 9 | 205 | 20 | 218 | 4.81e-33 | 118 |
| MS.gene25136.t1 | AT3G10080 | 37.755 | 196 | 104 | 7 | 22 | 205 | 30 | 219 | 2.09e-32 | 117 |
| MS.gene25136.t1 | AT3G04150 | 33.163 | 196 | 123 | 4 | 22 | 209 | 30 | 225 | 1.32e-31 | 115 |
| MS.gene25136.t1 | AT3G10080 | 37.755 | 196 | 104 | 7 | 22 | 205 | 101 | 290 | 1.53e-31 | 116 |
| MS.gene25136.t1 | AT4G14630 | 35.238 | 210 | 124 | 6 | 7 | 205 | 14 | 222 | 2.31e-31 | 114 |
| MS.gene25136.t1 | AT3G04150 | 34.444 | 180 | 111 | 3 | 37 | 209 | 59 | 238 | 2.62e-31 | 114 |
| MS.gene25136.t1 | AT5G38910 | 35.981 | 214 | 127 | 5 | 1 | 205 | 7 | 219 | 1.94e-30 | 112 |
| MS.gene25136.t1 | AT1G74820 | 34.225 | 187 | 117 | 4 | 23 | 205 | 41 | 225 | 3.64e-29 | 108 |
| MS.gene25136.t1 | AT5G61750 | 36.000 | 175 | 96 | 4 | 6 | 170 | 7 | 175 | 5.34e-29 | 107 |
| MS.gene25136.t1 | AT3G04170 | 33.803 | 213 | 121 | 7 | 7 | 205 | 12 | 218 | 1.55e-28 | 107 |
| MS.gene25136.t1 | AT5G38940 | 35.052 | 194 | 118 | 4 | 20 | 205 | 28 | 221 | 2.09e-28 | 106 |
| MS.gene25136.t1 | AT5G38940 | 35.052 | 194 | 118 | 4 | 20 | 205 | 52 | 245 | 3.83e-28 | 106 |
| MS.gene25136.t1 | AT5G38930 | 34.536 | 194 | 119 | 4 | 20 | 205 | 28 | 221 | 1.56e-27 | 104 |
| MS.gene25136.t1 | AT3G04190 | 31.959 | 194 | 124 | 4 | 20 | 205 | 28 | 221 | 1.70e-27 | 104 |
| MS.gene25136.t1 | AT3G04180 | 31.959 | 194 | 124 | 4 | 20 | 205 | 28 | 221 | 1.26e-25 | 99.4 |
| MS.gene25136.t1 | AT1G10460 | 36.220 | 127 | 78 | 3 | 60 | 183 | 65 | 191 | 1.92e-18 | 80.5 |
| MS.gene25136.t1 | AT5G38910 | 29.384 | 211 | 108 | 5 | 1 | 205 | 7 | 182 | 4.86e-15 | 70.5 |
Find 46 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATAGCCTGTAGGTGTTTC+TGG | 0.183963 | 8.4:+72905096 | None:intergenic |
| TGCTACCGAATTATTGATTA+TGG | 0.216281 | 8.4:-72904875 | MS.gene25136:CDS |
| GAAAGAGTTGTTAGTGTTTC+CGG | 0.229368 | 8.4:+72905017 | None:intergenic |
| GAATTATTGATTATGGTTAA+AGG | 0.256071 | 8.4:-72904868 | MS.gene25136:CDS |
| TAATTCAGAAGGTAAATTAT+TGG | 0.286814 | 8.4:+72904657 | None:intergenic |
| TTTCCAGCTCTTAACGATCT+TGG | 0.306429 | 8.4:-72904961 | MS.gene25136:CDS |
| TAATTTACCTTCTGAATTAG+TGG | 0.324310 | 8.4:-72904653 | MS.gene25136:CDS |
| GTTGAAACCGGGTGATCTTA+TGG | 0.324992 | 8.4:-72904797 | MS.gene25136:CDS |
| AAGAAGCTAAAGGCTCGGTT+TGG | 0.389106 | 8.4:-72904598 | MS.gene25136:CDS |
| TTATTCGAAGACGTTGAAAC+CGG | 0.390433 | 8.4:-72904809 | MS.gene25136:CDS |
| TTTAACCATAATCAATAATT+CGG | 0.394261 | 8.4:+72904870 | None:intergenic |
| TCAGATGATTTCGTCTTTCA+CGG | 0.399030 | 8.4:-72905048 | MS.gene25136:CDS |
| GTCTTTCACGGCTTTGTAGC+CGG | 0.402040 | 8.4:-72905036 | MS.gene25136:CDS |
| AGAAGCTAAAGGCTCGGTTT+GGG | 0.424746 | 8.4:-72904597 | MS.gene25136:CDS |
| TCTTCACTTGAGCAAGATCA+AGG | 0.452247 | 8.4:+72904617 | None:intergenic |
| ATCGTTAAGAGCTGGAAAAT+CGG | 0.453877 | 8.4:+72904966 | None:intergenic |
| TTTCAAGTTAATTCAGGTAA+GGG | 0.490682 | 8.4:-72904751 | MS.gene25136:CDS |
| GTTAAAGGTGAAATAACTGC+TGG | 0.507279 | 8.4:-72904853 | MS.gene25136:CDS |
| AGAGTGAAAAGGAAAAGTAT+AGG | 0.514106 | 8.4:+72905180 | None:intergenic |
| ATTCCAAGATCGTTAAGAGC+TGG | 0.523170 | 8.4:+72904958 | None:intergenic |
| ATCAATAATTCGGTAGCACC+AGG | 0.528604 | 8.4:+72904880 | None:intergenic |
| TATTCGAAGACGTTGAAACC+GGG | 0.533516 | 8.4:-72904808 | MS.gene25136:CDS |
| AGATCAAGTAATTGAGCACC+AGG | 0.545978 | 8.4:+72904691 | None:intergenic |
| ATTCCAATGCATACTCATCC+TGG | 0.548743 | 8.4:-72904898 | MS.gene25136:CDS |
| GCACCAGGATGAGTATGCAT+TGG | 0.553990 | 8.4:+72904895 | None:intergenic |
| GAAGCTAAAGGCTCGGTTTG+GGG | 0.563781 | 8.4:-72904596 | MS.gene25136:CDS |
| AAAATCATGGACAGAAGCAT+AGG | 0.563955 | 8.4:+72905140 | None:intergenic |
| TGCTCAAGTGAAGAAGCTAA+AGG | 0.572676 | 8.4:-72904608 | MS.gene25136:CDS |
| GTTTGTGCCACTAATTCAGA+AGG | 0.574335 | 8.4:+72904646 | None:intergenic |
| CATCTGATGTGATGTTTGCA+AGG | 0.576393 | 8.4:+72905064 | None:intergenic |
| AAGCTAAAGGCTCGGTTTGG+GGG | 0.585774 | 8.4:-72904595 | MS.gene25136:CDS |
| AAAGCTCCAGAAACACCTAC+AGG | 0.587844 | 8.4:-72905102 | MS.gene25136:CDS |
| GACAGAAGCATAGGAAGTGG+TGG | 0.591518 | 8.4:+72905149 | None:intergenic |
| ATGGACAGAAGCATAGGAAG+TGG | 0.593850 | 8.4:+72905146 | None:intergenic |
| TGAAAATGCAACATCCCTTG+TGG | 0.594793 | 8.4:+72904769 | None:intergenic |
| ACTAAAAGTAAGAAAAGCAG+TGG | 0.611069 | 8.4:+72904723 | None:intergenic |
| ATCTGATGTGATGTTTGCAA+GGG | 0.619647 | 8.4:+72905065 | None:intergenic |
| TGGATGAGAGAAGAGTGAAA+AGG | 0.620855 | 8.4:+72905169 | None:intergenic |
| AAGTGAAGAAGCTAAAGGCT+CGG | 0.621823 | 8.4:-72904603 | MS.gene25136:CDS |
| ATCTTGGAATCTCTGCGGCA+CGG | 0.632838 | 8.4:-72904945 | MS.gene25136:CDS |
| GCACGGTTAGACATAGCGAA+AGG | 0.667594 | 8.4:-72904928 | MS.gene25136:CDS |
| CGGTTAGACATAGCGAAAGG+CGG | 0.694402 | 8.4:-72904925 | MS.gene25136:CDS |
| GGCATGCAGTGATAGCCTGT+AGG | 0.702564 | 8.4:+72905087 | None:intergenic |
| TAACGATCTTGGAATCTCTG+CGG | 0.726837 | 8.4:-72904950 | MS.gene25136:CDS |
| TCTGATGTGATGTTTGCAAG+GGG | 0.732037 | 8.4:+72905066 | None:intergenic |
| AAAATCGGTGACAAACGCAG+AGG | 0.761602 | 8.4:+72904981 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAACCATAATCAATAATT+CGG | + | chr8.4:72904910-72904929 | None:intergenic | 15.0% |
| !! | TAATTCAGAAGGTAAATTAT+TGG | + | chr8.4:72905123-72905142 | None:intergenic | 20.0% |
| !!! | GAATTATTGATTATGGTTAA+AGG | - | chr8.4:72904909-72904928 | MS.gene25136:CDS | 20.0% |
| ! | TAATTTACCTTCTGAATTAG+TGG | - | chr8.4:72905124-72905143 | MS.gene25136:CDS | 25.0% |
| !!! | TTGCATTTTCAAGTTAATTC+AGG | - | chr8.4:72905020-72905039 | MS.gene25136:CDS | 25.0% |
| !!! | TTTCAAGTTAATTCAGGTAA+GGG | - | chr8.4:72905026-72905045 | MS.gene25136:CDS | 25.0% |
| !!! | TTTTCAAGTTAATTCAGGTA+AGG | - | chr8.4:72905025-72905044 | MS.gene25136:CDS | 25.0% |
| ACTAAAAGTAAGAAAAGCAG+TGG | + | chr8.4:72905057-72905076 | None:intergenic | 30.0% | |
| TATCTCCTACACAAAAATCA+TGG | + | chr8.4:72904653-72904672 | None:intergenic | 30.0% | |
| ! | AGAGTGAAAAGGAAAAGTAT+AGG | + | chr8.4:72904600-72904619 | None:intergenic | 30.0% |
| ! | ATCTTATGGTTTTTCCACAA+GGG | - | chr8.4:72904994-72905013 | MS.gene25136:CDS | 30.0% |
| ! | TGCTACCGAATTATTGATTA+TGG | - | chr8.4:72904902-72904921 | MS.gene25136:CDS | 30.0% |
| ATCGTTAAGAGCTGGAAAAT+CGG | + | chr8.4:72904814-72904833 | None:intergenic | 35.0% | |
| ATCTGATGTGATGTTTGCAA+GGG | + | chr8.4:72904715-72904734 | None:intergenic | 35.0% | |
| GAAAGAGTTGTTAGTGTTTC+CGG | + | chr8.4:72904763-72904782 | None:intergenic | 35.0% | |
| GTTAAAGGTGAAATAACTGC+TGG | - | chr8.4:72904924-72904943 | MS.gene25136:CDS | 35.0% | |
| TCAGATGATTTCGTCTTTCA+CGG | - | chr8.4:72904729-72904748 | MS.gene25136:CDS | 35.0% | |
| TTATTCGAAGACGTTGAAAC+CGG | - | chr8.4:72904968-72904987 | MS.gene25136:CDS | 35.0% | |
| ! | AAAATCATGGACAGAAGCAT+AGG | + | chr8.4:72904640-72904659 | None:intergenic | 35.0% |
| ! | ACTTTTAGTAGTGCAAATCC+TGG | - | chr8.4:72905068-72905087 | MS.gene25136:CDS | 35.0% |
| ! | GATCTTATGGTTTTTCCACA+AGG | - | chr8.4:72904993-72905012 | MS.gene25136:CDS | 35.0% |
| ! | TCTGTCCATGATTTTTGTGT+AGG | - | chr8.4:72904645-72904664 | MS.gene25136:CDS | 35.0% |
| AAGTGAAGAAGCTAAAGGCT+CGG | - | chr8.4:72905174-72905193 | MS.gene25136:CDS | 40.0% | |
| AGATCAAGTAATTGAGCACC+AGG | + | chr8.4:72905089-72905108 | None:intergenic | 40.0% | |
| ATCAATAATTCGGTAGCACC+AGG | + | chr8.4:72904900-72904919 | None:intergenic | 40.0% | |
| ATTCCAAGATCGTTAAGAGC+TGG | + | chr8.4:72904822-72904841 | None:intergenic | 40.0% | |
| ATTCCAATGCATACTCATCC+TGG | - | chr8.4:72904879-72904898 | MS.gene25136:CDS | 40.0% | |
| CATCTGATGTGATGTTTGCA+AGG | + | chr8.4:72904716-72904735 | None:intergenic | 40.0% | |
| GGAAAAACCATAAGATCACC+CGG | + | chr8.4:72904990-72905009 | None:intergenic | 40.0% | |
| TAACGATCTTGGAATCTCTG+CGG | - | chr8.4:72904827-72904846 | MS.gene25136:CDS | 40.0% | |
| TATTCGAAGACGTTGAAACC+GGG | - | chr8.4:72904969-72904988 | MS.gene25136:CDS | 40.0% | |
| TCTGATGTGATGTTTGCAAG+GGG | + | chr8.4:72904714-72904733 | None:intergenic | 40.0% | |
| TCTTCACTTGAGCAAGATCA+AGG | + | chr8.4:72905163-72905182 | None:intergenic | 40.0% | |
| TGAAAATGCAACATCCCTTG+TGG | + | chr8.4:72905011-72905030 | None:intergenic | 40.0% | |
| TGCTCAAGTGAAGAAGCTAA+AGG | - | chr8.4:72905169-72905188 | MS.gene25136:CDS | 40.0% | |
| TGGATGAGAGAAGAGTGAAA+AGG | + | chr8.4:72904611-72904630 | None:intergenic | 40.0% | |
| TTTCCAGCTCTTAACGATCT+TGG | - | chr8.4:72904816-72904835 | MS.gene25136:CDS | 40.0% | |
| ! | GTTTGTGCCACTAATTCAGA+AGG | + | chr8.4:72905134-72905153 | None:intergenic | 40.0% |
| AAAATCGGTGACAAACGCAG+AGG | + | chr8.4:72904799-72904818 | None:intergenic | 45.0% | |
| AAAGCTCCAGAAACACCTAC+AGG | - | chr8.4:72904675-72904694 | MS.gene25136:CDS | 45.0% | |
| GTTGAAACCGGGTGATCTTA+TGG | - | chr8.4:72904980-72904999 | MS.gene25136:CDS | 45.0% | |
| TGATAGCCTGTAGGTGTTTC+TGG | + | chr8.4:72904684-72904703 | None:intergenic | 45.0% | |
| ! | AAGAAGCTAAAGGCTCGGTT+TGG | - | chr8.4:72905179-72905198 | MS.gene25136:CDS | 45.0% |
| ! | AGAAGCTAAAGGCTCGGTTT+GGG | - | chr8.4:72905180-72905199 | MS.gene25136:CDS | 45.0% |
| ! | ATGGACAGAAGCATAGGAAG+TGG | + | chr8.4:72904634-72904653 | None:intergenic | 45.0% |
| ATCTTGGAATCTCTGCGGCA+CGG | - | chr8.4:72904832-72904851 | MS.gene25136:CDS | 50.0% | |
| CGGTTAGACATAGCGAAAGG+CGG | - | chr8.4:72904852-72904871 | MS.gene25136:CDS | 50.0% | |
| GCACCAGGATGAGTATGCAT+TGG | + | chr8.4:72904885-72904904 | None:intergenic | 50.0% | |
| GCACGGTTAGACATAGCGAA+AGG | - | chr8.4:72904849-72904868 | MS.gene25136:CDS | 50.0% | |
| ! | GAAGCTAAAGGCTCGGTTTG+GGG | - | chr8.4:72905181-72905200 | MS.gene25136:CDS | 50.0% |
| ! | GACAGAAGCATAGGAAGTGG+TGG | + | chr8.4:72904631-72904650 | None:intergenic | 50.0% |
| ! | GTCTTTCACGGCTTTGTAGC+CGG | - | chr8.4:72904741-72904760 | MS.gene25136:CDS | 50.0% |
| !! | AAGCTAAAGGCTCGGTTTGG+GGG | - | chr8.4:72905182-72905201 | MS.gene25136:CDS | 50.0% |
| GGCATGCAGTGATAGCCTGT+AGG | + | chr8.4:72904693-72904712 | None:intergenic | 55.0% | |
| !! | AAGGCTCGGTTTGGGGGAAG+AGG | - | chr8.4:72905188-72905207 | MS.gene25136:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 72904585 | 72905214 | 72904585 | ID=MS.gene25136 |
| chr8.4 | mRNA | 72904585 | 72905214 | 72904585 | ID=MS.gene25136.t1;Parent=MS.gene25136 |
| chr8.4 | exon | 72904585 | 72905214 | 72904585 | ID=MS.gene25136.t1.exon1;Parent=MS.gene25136.t1 |
| chr8.4 | CDS | 72904585 | 72905214 | 72904585 | ID=cds.MS.gene25136.t1;Parent=MS.gene25136.t1 |
| Gene Sequence |
| Protein sequence |
