Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25190.t1 | XP_024638642.1 | 90 | 130 | 10 | 1 | 1 | 127 | 1 | 130 | 1.10E-53 | 219.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25190.t1 | Q9CX48 | 58.8 | 34 | 14 | 0 | 83 | 116 | 20 | 53 | 2.6e-06 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25190.t1 | A0A396IFI3 | 90.0 | 130 | 10 | 1 | 1 | 127 | 1 | 130 | 8.1e-54 | 219.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045256 | MS.gene25190 | PPI |
| MS.gene75592 | MS.gene25190 | PPI |
| MS.gene25190 | MS.gene066824 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25190.t1 | MTR_1g032030 | 91.935 | 62 | 5 | 0 | 19 | 80 | 16 | 77 | 2.23e-23 | 92.0 |
| MS.gene25190.t1 | MTR_1g032370 | 91.935 | 62 | 5 | 0 | 19 | 80 | 16 | 77 | 2.23e-23 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25190.t1 | AT5G49400 | 70.714 | 140 | 27 | 2 | 1 | 126 | 1 | 140 | 3.64e-48 | 159 |
Find 53 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACACATCCGGCCTCAATTT+AGG | 0.222584 | 1.3:+17282386 | None:intergenic |
| ACACATCCGGCCTCAATTTA+GGG | 0.281606 | 1.3:+17282387 | None:intergenic |
| AGCCGCAGTTGCAGCTCTTT+CGG | 0.299402 | 1.3:+17282664 | None:intergenic |
| ATCAGATTATTCTTCTAGTT+CGG | 0.307716 | 1.3:-17282228 | MS.gene25190:CDS |
| TAGTAAGAAGGAAGAGATTA+AGG | 0.323676 | 1.3:-17282729 | MS.gene25190:CDS |
| TCAGATTATTCTTCTAGTTC+GGG | 0.333573 | 1.3:-17282227 | MS.gene25190:CDS |
| GTCCAAGAGGAAGCATGATT+CGG | 0.390952 | 1.3:-17282288 | MS.gene25190:CDS |
| TCCAAGAGGAAGCATGATTC+GGG | 0.420579 | 1.3:-17282287 | MS.gene25190:CDS |
| TCCTTCCTTTCACCCACATT+AGG | 0.428626 | 1.3:+17282536 | None:intergenic |
| GCAGTATTAACTGCTGCGAC+AGG | 0.437303 | 1.3:-17282704 | MS.gene25190:CDS |
| ACCCGAATCATGCTTCCTCT+TGG | 0.441226 | 1.3:+17282286 | None:intergenic |
| GCGCGTAATGTTAACGCATA+TGG | 0.469597 | 1.3:-17282641 | MS.gene25190:CDS |
| CTCTGATTCAGACGATGAGC+TGG | 0.469980 | 1.3:+17282202 | None:intergenic |
| CAGAGGAAGAAAGGAGAAGG+AGG | 0.477099 | 1.3:-17282184 | MS.gene25190:CDS |
| ATGAGCGGGTTTATATTTCA+AGG | 0.478841 | 1.3:-17282436 | MS.gene25190:CDS |
| CGTCTGAATCAGAGGAAGAA+AGG | 0.505020 | 1.3:-17282193 | MS.gene25190:CDS |
| GAATATGTCGAGTAGTAAGA+AGG | 0.505551 | 1.3:-17282741 | None:intergenic |
| CATGATTCGGGTATTGATAG+TGG | 0.509658 | 1.3:-17282275 | MS.gene25190:CDS |
| AGTGTTACCAGGGTGGGCAC+TGG | 0.514214 | 1.3:-17282475 | MS.gene25190:CDS |
| TCGTGATGTGAAGGAGGAAA+AGG | 0.515250 | 1.3:-17282330 | MS.gene25190:CDS |
| GCAGTGTCAGAAGTGTTACC+AGG | 0.520961 | 1.3:-17282486 | MS.gene25190:CDS |
| GCATATGGTCAGAAGGAAGA+AGG | 0.525781 | 1.3:-17282626 | MS.gene25190:CDS |
| TCGGGTATTGATAGTGGCAG+TGG | 0.535036 | 1.3:-17282269 | MS.gene25190:CDS |
| AGAAGGGCCTAGCAGACGGC+AGG | 0.535228 | 1.3:-17282609 | MS.gene25190:CDS |
| TCATAACCCACAGACACATC+CGG | 0.535556 | 1.3:+17282374 | None:intergenic |
| TCCTAATGTGGGTGAAAGGA+AGG | 0.545084 | 1.3:-17282537 | MS.gene25190:CDS |
| AGTGGATCATCGTCTGCCAC+AGG | 0.546556 | 1.3:-17282251 | MS.gene25190:CDS |
| AGCCGAAAGAGCTGCAACTG+CGG | 0.555552 | 1.3:-17282666 | MS.gene25190:CDS |
| TTAAGTTGCTGAGTCCTCGA+TGG | 0.557112 | 1.3:+17282413 | None:intergenic |
| CAGCTCATCGTCTGAATCAG+AGG | 0.557858 | 1.3:-17282201 | MS.gene25190:CDS |
| ATTGAGGCCGGATGTGTCTG+TGG | 0.567581 | 1.3:-17282381 | MS.gene25190:CDS |
| AAGCTCCTAATGTGGGTGAA+AGG | 0.572605 | 1.3:-17282541 | MS.gene25190:CDS |
| CAGTATTAACTGCTGCGACA+GGG | 0.573455 | 1.3:-17282703 | MS.gene25190:CDS |
| AGGAAGAAGGGCCTAGCAGA+CGG | 0.577723 | 1.3:-17282613 | MS.gene25190:CDS |
| CATATCAATGTAAGAATGAG+CGG | 0.579082 | 1.3:-17282451 | MS.gene25190:CDS |
| TGTTAACGCATATGGTCAGA+AGG | 0.582981 | 1.3:-17282633 | MS.gene25190:CDS |
| TAATAATCATCGTGATGTGA+AGG | 0.585747 | 1.3:-17282339 | MS.gene25190:CDS |
| ATATCAATGTAAGAATGAGC+GGG | 0.601513 | 1.3:-17282450 | MS.gene25190:CDS |
| GTCAGAAGTGTTACCAGGGT+GGG | 0.604446 | 1.3:-17282481 | MS.gene25190:CDS |
| CAGACTAAGAAGAAGAGCAA+AGG | 0.608854 | 1.3:-17282134 | MS.gene25190:CDS |
| TAATCATCGTGATGTGAAGG+AGG | 0.609023 | 1.3:-17282336 | MS.gene25190:CDS |
| AATCAGAGGAAGAAAGGAGA+AGG | 0.616394 | 1.3:-17282187 | MS.gene25190:CDS |
| CATATGGTCAGAAGGAAGAA+GGG | 0.616780 | 1.3:-17282625 | MS.gene25190:CDS |
| TACTGAGAAAGCTCCTAATG+TGG | 0.619802 | 1.3:-17282549 | MS.gene25190:CDS |
| TGCGCATTACCTTCGAGTGT+AGG | 0.623388 | 1.3:+17282506 | None:intergenic |
| GATGTCAAGCCTACACTCGA+AGG | 0.631697 | 1.3:-17282515 | MS.gene25190:CDS |
| ACTGAGAAAGCTCCTAATGT+GGG | 0.641132 | 1.3:-17282548 | MS.gene25190:CDS |
| GATATGTCCAGTGCCCACCC+TGG | 0.650305 | 1.3:+17282468 | None:intergenic |
| TGTCAGAAGTGTTACCAGGG+TGG | 0.665235 | 1.3:-17282482 | MS.gene25190:CDS |
| CAGTGTCAGAAGTGTTACCA+GGG | 0.669839 | 1.3:-17282485 | MS.gene25190:CDS |
| AGAAGAATAATCTGATCCTG+TGG | 0.671542 | 1.3:+17282235 | None:intergenic |
| TTGAGGCCGGATGTGTCTGT+GGG | 0.683391 | 1.3:-17282380 | MS.gene25190:CDS |
| TTTATATTTCAAGGCCATCG+AGG | 0.711495 | 1.3:-17282427 | MS.gene25190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTTAAAAACCCTAAATTG+AGG | - | chr1.3:17282449-17282468 | MS.gene25190:CDS | 25.0% |
| !! | ATCAGATTATTCTTCTAGTT+CGG | - | chr1.3:17282618-17282637 | MS.gene25190:CDS | 25.0% |
| ATATCAATGTAAGAATGAGC+GGG | - | chr1.3:17282396-17282415 | MS.gene25190:CDS | 30.0% | |
| CATATCAATGTAAGAATGAG+CGG | - | chr1.3:17282395-17282414 | MS.gene25190:CDS | 30.0% | |
| TAATAATCATCGTGATGTGA+AGG | - | chr1.3:17282507-17282526 | MS.gene25190:CDS | 30.0% | |
| TAGTAAGAAGGAAGAGATTA+AGG | - | chr1.3:17282117-17282136 | MS.gene25190:CDS | 30.0% | |
| !! | TCAGATTATTCTTCTAGTTC+GGG | - | chr1.3:17282619-17282638 | MS.gene25190:CDS | 30.0% |
| AGAAGAATAATCTGATCCTG+TGG | + | chr1.3:17282614-17282633 | None:intergenic | 35.0% | |
| TAAAAACCCTAAATTGAGGC+CGG | - | chr1.3:17282453-17282472 | MS.gene25190:CDS | 35.0% | |
| TTTATATTTCAAGGCCATCG+AGG | - | chr1.3:17282419-17282438 | MS.gene25190:CDS | 35.0% | |
| ! | ATGAGCGGGTTTATATTTCA+AGG | - | chr1.3:17282410-17282429 | MS.gene25190:CDS | 35.0% |
| AATCAGAGGAAGAAAGGAGA+AGG | - | chr1.3:17282659-17282678 | MS.gene25190:CDS | 40.0% | |
| ACTGAGAAAGCTCCTAATGT+GGG | - | chr1.3:17282298-17282317 | MS.gene25190:CDS | 40.0% | |
| AGAAGAGCAAAGGAAAAAGC+AGG | - | chr1.3:17282722-17282741 | MS.gene25190:CDS | 40.0% | |
| CAGACTAAGAAGAAGAGCAA+AGG | - | chr1.3:17282712-17282731 | MS.gene25190:CDS | 40.0% | |
| CATATGGTCAGAAGGAAGAA+GGG | - | chr1.3:17282221-17282240 | MS.gene25190:CDS | 40.0% | |
| TAATCATCGTGATGTGAAGG+AGG | - | chr1.3:17282510-17282529 | MS.gene25190:CDS | 40.0% | |
| TACTGAGAAAGCTCCTAATG+TGG | - | chr1.3:17282297-17282316 | MS.gene25190:CDS | 40.0% | |
| TGAGTTCGAAAAAGTCCAAG+AGG | - | chr1.3:17282545-17282564 | MS.gene25190:CDS | 40.0% | |
| TGTTAACGCATATGGTCAGA+AGG | - | chr1.3:17282213-17282232 | MS.gene25190:CDS | 40.0% | |
| !! | CATGATTCGGGTATTGATAG+TGG | - | chr1.3:17282571-17282590 | MS.gene25190:CDS | 40.0% |
| AAGCTCCTAATGTGGGTGAA+AGG | - | chr1.3:17282305-17282324 | MS.gene25190:CDS | 45.0% | |
| ACACATCCGGCCTCAATTTA+GGG | + | chr1.3:17282462-17282481 | None:intergenic | 45.0% | |
| AGAGCAAAGGAAAAAGCAGG+AGG | - | chr1.3:17282725-17282744 | MS.gene25190:CDS | 45.0% | |
| CAGTATTAACTGCTGCGACA+GGG | - | chr1.3:17282143-17282162 | MS.gene25190:CDS | 45.0% | |
| CGTCTGAATCAGAGGAAGAA+AGG | - | chr1.3:17282653-17282672 | MS.gene25190:CDS | 45.0% | |
| GCATATGGTCAGAAGGAAGA+AGG | - | chr1.3:17282220-17282239 | MS.gene25190:CDS | 45.0% | |
| GCGCGTAATGTTAACGCATA+TGG | - | chr1.3:17282205-17282224 | MS.gene25190:CDS | 45.0% | |
| TCATAACCCACAGACACATC+CGG | + | chr1.3:17282475-17282494 | None:intergenic | 45.0% | |
| TCCTAATGTGGGTGAAAGGA+AGG | - | chr1.3:17282309-17282328 | MS.gene25190:CDS | 45.0% | |
| TCCTTCCTTTCACCCACATT+AGG | + | chr1.3:17282313-17282332 | None:intergenic | 45.0% | |
| TCGTGATGTGAAGGAGGAAA+AGG | - | chr1.3:17282516-17282535 | MS.gene25190:CDS | 45.0% | |
| ! | GTCCAAGAGGAAGCATGATT+CGG | - | chr1.3:17282558-17282577 | MS.gene25190:CDS | 45.0% |
| ! | TCCAAGAGGAAGCATGATTC+GGG | - | chr1.3:17282559-17282578 | MS.gene25190:CDS | 45.0% |
| ! | TTAAGTTGCTGAGTCCTCGA+TGG | + | chr1.3:17282436-17282455 | None:intergenic | 45.0% |
| !! | CAGTGTCAGAAGTGTTACCA+GGG | - | chr1.3:17282361-17282380 | MS.gene25190:CDS | 45.0% |
| ACCCGAATCATGCTTCCTCT+TGG | + | chr1.3:17282563-17282582 | None:intergenic | 50.0% | |
| AGGAAAAACAGGAGGCGAAG+AGG | - | chr1.3:17282257-17282276 | MS.gene25190:CDS | 50.0% | |
| CAGAGGAAGAAAGGAGAAGG+AGG | - | chr1.3:17282662-17282681 | MS.gene25190:CDS | 50.0% | |
| CAGCTCATCGTCTGAATCAG+AGG | - | chr1.3:17282645-17282664 | MS.gene25190:CDS | 50.0% | |
| CTCTGATTCAGACGATGAGC+TGG | + | chr1.3:17282647-17282666 | None:intergenic | 50.0% | |
| GACACATCCGGCCTCAATTT+AGG | + | chr1.3:17282463-17282482 | None:intergenic | 50.0% | |
| GATGTCAAGCCTACACTCGA+AGG | - | chr1.3:17282331-17282350 | MS.gene25190:CDS | 50.0% | |
| GCAGTATTAACTGCTGCGAC+AGG | - | chr1.3:17282142-17282161 | MS.gene25190:CDS | 50.0% | |
| TAGCAGACGGCAGGAAAAAC+AGG | - | chr1.3:17282246-17282265 | MS.gene25190:CDS | 50.0% | |
| TGCGCATTACCTTCGAGTGT+AGG | + | chr1.3:17282343-17282362 | None:intergenic | 50.0% | |
| ! | TGTTTTTCCTGCCGTCTGCT+AGG | + | chr1.3:17282247-17282266 | None:intergenic | 50.0% |
| !! | GCAGTGTCAGAAGTGTTACC+AGG | - | chr1.3:17282360-17282379 | MS.gene25190:CDS | 50.0% |
| !! | GTCAGAAGTGTTACCAGGGT+GGG | - | chr1.3:17282365-17282384 | MS.gene25190:CDS | 50.0% |
| !! | TCGGGTATTGATAGTGGCAG+TGG | - | chr1.3:17282577-17282596 | MS.gene25190:CDS | 50.0% |
| !! | TGTCAGAAGTGTTACCAGGG+TGG | - | chr1.3:17282364-17282383 | MS.gene25190:CDS | 50.0% |
| AGCCGAAAGAGCTGCAACTG+CGG | - | chr1.3:17282180-17282199 | MS.gene25190:CDS | 55.0% | |
| AGCCGCAGTTGCAGCTCTTT+CGG | + | chr1.3:17282185-17282204 | None:intergenic | 55.0% | |
| AGGAAGAAGGGCCTAGCAGA+CGG | - | chr1.3:17282233-17282252 | MS.gene25190:CDS | 55.0% | |
| AGTGGATCATCGTCTGCCAC+AGG | - | chr1.3:17282595-17282614 | MS.gene25190:CDS | 55.0% | |
| ATTGAGGCCGGATGTGTCTG+TGG | - | chr1.3:17282465-17282484 | MS.gene25190:CDS | 55.0% | |
| CAGACGGCAGGAAAAACAGG+AGG | - | chr1.3:17282249-17282268 | MS.gene25190:CDS | 55.0% | |
| TTGAGGCCGGATGTGTCTGT+GGG | - | chr1.3:17282466-17282485 | MS.gene25190:CDS | 55.0% | |
| AGTGTTACCAGGGTGGGCAC+TGG | - | chr1.3:17282371-17282390 | MS.gene25190:CDS | 60.0% | |
| GATATGTCCAGTGCCCACCC+TGG | + | chr1.3:17282381-17282400 | None:intergenic | 60.0% | |
| AGAAGGGCCTAGCAGACGGC+AGG | - | chr1.3:17282237-17282256 | MS.gene25190:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 17282109 | 17282759 | 17282109 | ID=MS.gene25190 |
| chr1.3 | mRNA | 17282109 | 17282759 | 17282109 | ID=MS.gene25190.t1;Parent=MS.gene25190 |
| chr1.3 | exon | 17282109 | 17282759 | 17282109 | ID=MS.gene25190.t1.exon1;Parent=MS.gene25190.t1 |
| chr1.3 | CDS | 17282109 | 17282759 | 17282109 | ID=cds.MS.gene25190.t1;Parent=MS.gene25190.t1 |
| Gene Sequence |
| Protein sequence |
