AlfalfaGEDB | MS.gene25653

M. sativa cultivar XinJiangDaYe / MS.gene25653

KEGG: K14486
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009719 GO:0009725 GO:0009889 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02362.24 PF06507.16

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25653.t1	XP_003609970.1	99.4	328	2	0	1	328	53	380	1.50E-190	675.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25653.t1	P93022	89.3	328	35	0	1	328	54	381	3.0e-176	619.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25653.t1	G7JS69	99.4	328	2	0	1	328	53	380	1.1e-190	675.2

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene25653.t1	TF	B3-ARF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052358	MS.gene25653	0.802885	4.54E-49	-1.69E-46
MS.gene055784	MS.gene25653	0.80372	3.05E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25653.t1	MTR_4g124900	99.390	328	2	0	1	328	53	380	0.0	701
MS.gene25653.t1	MTR_4g124900	99.390	328	2	0	1	328	53	380	0.0	701
MS.gene25653.t1	MTR_2g043250	91.159	328	29	0	1	328	53	380	0.0	590
MS.gene25653.t1	MTR_8g101360	79.091	330	67	2	1	328	61	390	2.06e-159	474
MS.gene25653.t1	MTR_2g018690	70.783	332	91	6	1	328	53	382	4.54e-156	460
MS.gene25653.t1	MTR_3g064050	69.394	330	95	6	1	326	56	383	1.69e-155	457
MS.gene25653.t1	MTR_5g076270	70.000	330	93	6	1	326	54	381	1.22e-154	454
MS.gene25653.t1	MTR_8g079492	70.181	332	92	7	1	328	53	381	1.96e-153	453
MS.gene25653.t1	MTR_8g079492	70.181	332	92	7	1	328	53	381	2.03e-153	453
MS.gene25653.t1	MTR_1g024025	71.475	305	84	3	1	303	73	376	3.29e-150	454

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25653.t1	AT5G20730	89.329	328	35	0	1	328	54	381	0.0	595
MS.gene25653.t1	AT1G19220	88.110	328	39	0	1	328	53	380	0.0	593
MS.gene25653.t1	AT5G20730	89.058	329	35	1	1	328	54	382	0.0	591
MS.gene25653.t1	AT5G20730	89.058	329	35	1	1	328	54	382	0.0	590
MS.gene25653.t1	AT1G30330	71.687	332	88	6	1	328	53	382	7.90e-158	465
MS.gene25653.t1	AT1G30330	71.687	332	88	6	1	328	55	384	9.07e-158	465
MS.gene25653.t1	AT1G30330	71.687	332	88	6	1	328	55	384	9.07e-158	465
MS.gene25653.t1	AT1G19850	70.261	306	87	4	1	303	84	388	9.56e-151	446
MS.gene25653.t1	AT1G19850	70.261	306	87	4	1	303	73	377	1.11e-150	446
MS.gene25653.t1	AT5G37020	66.667	330	104	6	1	326	52	379	2.65e-148	436

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Find 74 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATTCGGTAGGTTGA+CGG	0.504207	8.2:+8054022	None:intergenic
AACACCTTCTTCTAGGCTTG+AGG	0.569429	8.2:-8053160	MS.gene25653:CDS
AAGAGACACTTGAGTGTACA+TGG	0.494633	8.2:+8052860	None:intergenic
AAGAGACATCTGTTGACTAC+TGG	0.463007	8.2:-8053376	MS.gene25653:CDS
AAGATCTGCATGACAATACA+TGG	0.486770	8.2:-8053761	MS.gene25653:CDS
AATAACAAATTCGGAGGGGC+TGG	0.488782	8.2:+8052905	None:intergenic
AATCAACTTGGATGGAAGAT+TGG	0.404278	8.2:+8054443	None:intergenic
AATGGAATAACAAATTCGGA+GGG	0.651060	8.2:+8052900	None:intergenic
ACCTGGCATGCCAGGGTGGC+CGG	0.323931	8.2:+8052496	None:intergenic
AGATACATGGGTACAGTTAC+AGG	0.345844	8.2:-8052789	MS.gene25653:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAATTCTCAAACTGAAG+AGG	+	chr8.2:8052798-8052817	None:intergenic	30.0%
AATAAGCTCATGAAGAAGTT+CGG	+	chr8.2:8054325-8054344	None:intergenic	30.0%
AATGGAATAACAAATTCGGA+GGG	+	chr8.2:8054090-8054109	None:intergenic	30.0%
ACAAATTCTCAAACTGAAGA+GGG	+	chr8.2:8052797-8052816	None:intergenic	30.0%
AGAAAACGGTCAATTTAACA+GGG	-	chr8.2:8054059-8054078	MS.gene25653:CDS	30.0%
ATAGTTACTCATCATTTGCT+TGG	+	chr8.2:8052866-8052885	None:intergenic	30.0%
CATAGACAAGAAAAACCAAA+AGG	+	chr8.2:8053144-8053163	None:intergenic	30.0%
GCGTTTAGACATATTTATAG+AGG	-	chr8.2:8053249-8053268	MS.gene25653:intron	30.0%
TCCATCAACTTGTGATTTAT+TGG	-	chr8.2:8052684-8052703	MS.gene25653:intron	30.0%
TGCTGAAATTGATTGAACAT+TGG	-	chr8.2:8053574-8053593	MS.gene25653:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	8052498	8054511	8052498	ID=MS.gene25653
chr8.2	mRNA	8052498	8054511	8052498	ID=MS.gene25653.t1;Parent=MS.gene25653
chr8.2	exon	8054416	8054511	8054416	ID=MS.gene25653.t1.exon1;Parent=MS.gene25653.t1
chr8.2	CDS	8054416	8054511	8054416	ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2	exon	8054223	8054279	8054223	ID=MS.gene25653.t1.exon2;Parent=MS.gene25653.t1
chr8.2	CDS	8054223	8054279	8054223	ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2	exon	8053936	8054091	8053936	ID=MS.gene25653.t1.exon3;Parent=MS.gene25653.t1
chr8.2	CDS	8053936	8054091	8053936	ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2	exon	8053739	8053823	8053739	ID=MS.gene25653.t1.exon4;Parent=MS.gene25653.t1
chr8.2	CDS	8053739	8053823	8053739	ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2	exon	8053316	8053406	8053316	ID=MS.gene25653.t1.exon5;Parent=MS.gene25653.t1
chr8.2	CDS	8053316	8053406	8053316	ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2	exon	8053029	8053193	8053029	ID=MS.gene25653.t1.exon6;Parent=MS.gene25653.t1
chr8.2	CDS	8053029	8053193	8053029	ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2	exon	8052734	8052929	8052734	ID=MS.gene25653.t1.exon7;Parent=MS.gene25653.t1
chr8.2	CDS	8052734	8052929	8052734	ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2	exon	8052498	8052636	8052498	ID=MS.gene25653.t1.exon8;Parent=MS.gene25653.t1
chr8.2	CDS	8052498	8052636	8052498	ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1

Gene Sequence

>MS.gene25653

GTTGCGGCTTCCATGCAAAAACAGACCGACTTCATACCCAGTTACCCCAATCTTCCATCCAAGTTGATTTGCATGCTTCACAATGTTGCCCTGCATGTAGGTGCATTTTCCGCTTGTGATGTGGCTGTTATGCAATAATGAAAACTTGTGAAGAAGAATTACTTATATACAGTTTATGATTTATATTCCATCAACTTGTGATTTATTGGTGTTTTTGTTTATTTTGGAGCAGGCTGATCCTGAAACTGACGAGGTCTACGCGCAAATGACACTTCAACCTGTAAACAAAGTAGGTTCCCTCTTCAGTTTGAGAATTTGTTATTGCGTCGCATGTTTAGTTCCTTACTTTTCTTTGACATCCTCTTCCAAGCAAATGATGAGTAACTATACAAAAGATTGTCTCATTTGTCTCTTCTGCAGTATGACAAGGACGCAATACTGGCATCAGATTTTGGTCTCAAACAAAACCGTCAACCTACCGAATTCTTTTGCAAAACTCTAACTGCTAGTGACACAAGCACTCATGGTGGATTTTCTGTTCCTCGTAGAGCAGCTGAGAAAATATTCCCACCTTTGGTGTGTATACAACATTCTCATATCTCTTGATTCATTTTCGCAAAATTTGGTAGAGCTTTATCTGATCCCTTTTGGTTTTTCTTGTCTATGCTGACCATAAGTGTTTTGATAGGATTTTTCAATGCAACCTCCCGCTCAGGAGCTTGTTGCTAAAGATCTGCATGACAATACATGGGCGTTTAGACATATTTATAGAGGTATTTCCACTTTTATTTTAGATATTCTTCATTCATTGCCATCTTACATTCATATATAATCATTAGTATATTGTATGCTTTATTGACGCACCTTTACATTCTATCTTTCTAAACCCTGTAGGTATATATTATCTTGTTATTGGTATGACAATGACATCACTTTTGTAAAAATGAAGCACATGCTAAAAATTGAGACTGTCCATACAGAAGAATTTTTTTTCAATAATCTTAGAACCGTCTTGTAAATGATTTGACCAATATACAAATATAAAGCTGTGTAATTTATGTGTTATGAAGTTTGAATGCTGAAATTGATTGAACATTGGCTACAGGGCAACCAAAGAGACATCTGTTGACTACTGGATGGAGTGTATTTATCAGCACAAAGAGACTTTTTGCTGGAGATTCTGTTCTTTTTATCAGGTAAGATATATATATATATATATATATATATATATATATATATATATATATATATCTTGTGTTGGAATTTCAGAGAATTTTTAACCACAGTTTCTGAGCAAATTTTTGTTGATTACTTTTAGAGATGAAAAACAACACCTTCTTCTAGGCTTGAGGCGCGCTAATAGACAGCAGCCAGCGCTTTCTTCATCAGTAATATCCAGTGACAGTATGCATATTGGCATTTTAGCTGCTGCCGCTCATGCTGCTGCAAATAACAGTCCATTTACTATATATTACAATCCAAGGTACTATTTGGCATGTATGTGTATTATCATACATAATATGGAATAGCATCCATCGAAATATTTGTGACAGTCTCTTACAGAAAACGGTCAATTTAACAGGGCCAGCCCCTCCGAATTTGTTATTCCATTGGCCAAGTATAACAAAGCCATGTACACTCAAGTGTCTCTTGGAATGAGATTTAGAATGATGTTTGAAACAGAGGAGTCTGGAGTACGTAGATACATGGGTACAGTTACAGGAATTAGTGACTTGGATCCTGTCCGATGGAAAAATTCACAATGGCGCAATCTTCAGGTATTATTAAAAGGTGTATTATAGTACTTGTTTTGATGTACTAATACCGAACTTCTTCATGAGCTTATTGGAGCAATTATTTTCGGACTATTTACAGGTTGGATGGGATGAATCGACAGCTGGTGAACGCCCAAGTAGAGTTTCGATTTGGGATATTGAACCAGTAGTGACTCCTTTCTATATTTGTCCACCTCCATTTTTCAGGCAAAACTTCCCCGGCCACCCTGGCATGCCAG

Protein sequence

>MS.gene25653.t1

VAASMQKQTDFIPSYPNLPSKLICMLHNVALHADPETDEVYAQMTLQPVNKYDKDAILASDFGLKQNRQPTEFFCKTLTASDTSTHGGFSVPRRAAEKIFPPLDFSMQPPAQELVAKDLHDNTWAFRHIYRGQPKRHLLTTGWSVFISTKRLFAGDSVLFIRDEKQHLLLGLRRANRQQPALSSSVISSDSMHIGILAAAAHAAANNSPFTIYYNPRASPSEFVIPLAKYNKAMYTQVSLGMRFRMMFETEESGVRRYMGTVTGISDLDPVRWKNSQWRNLQVGWDESTAGERPSRVSIWDIEPVVTPFYICPPPFFRQNFPGHPGMP