AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25653

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25653.t1 MTR_4g124900 99.390 328 2 0 1 328 53 380 0.0 701
MS.gene25653.t1 MTR_4g124900 99.390 328 2 0 1 328 53 380 0.0 701
MS.gene25653.t1 MTR_2g043250 91.159 328 29 0 1 328 53 380 0.0 590
MS.gene25653.t1 MTR_8g101360 79.091 330 67 2 1 328 61 390 2.06e-159 474
MS.gene25653.t1 MTR_2g018690 70.783 332 91 6 1 328 53 382 4.54e-156 460
MS.gene25653.t1 MTR_3g064050 69.394 330 95 6 1 326 56 383 1.69e-155 457
MS.gene25653.t1 MTR_5g076270 70.000 330 93 6 1 326 54 381 1.22e-154 454
MS.gene25653.t1 MTR_8g079492 70.181 332 92 7 1 328 53 381 1.96e-153 453
MS.gene25653.t1 MTR_8g079492 70.181 332 92 7 1 328 53 381 2.03e-153 453
MS.gene25653.t1 MTR_1g024025 71.475 305 84 3 1 303 73 376 3.29e-150 454
MS.gene25653.t1 MTR_1g024025 69.880 83 21 2 163 244 861 940 1.60e-21 95.9
MS.gene25653.t1 MTR_8g079492 72.425 301 78 5 31 328 1 299 2.36e-144 427
MS.gene25653.t1 MTR_8g100050 51.713 321 154 1 2 322 91 410 6.10e-112 341
MS.gene25653.t1 MTR_8g100050 51.713 321 154 1 2 322 91 410 7.88e-111 341
MS.gene25653.t1 MTR_2g005240 53.535 297 134 3 12 307 65 358 2.44e-107 327
MS.gene25653.t1 MTR_2g014770 49.026 308 148 1 6 304 81 388 1.39e-97 301
MS.gene25653.t1 MTR_2g093740 50.654 306 135 5 12 304 97 399 3.04e-97 302
MS.gene25653.t1 MTR_2g014770 49.026 308 148 1 6 304 81 388 6.46e-97 301
MS.gene25653.t1 MTR_2g093740 50.654 306 135 5 12 304 97 399 1.29e-96 302
MS.gene25653.t1 MTR_2g093740 50.654 306 135 5 12 304 97 399 4.07e-96 302
MS.gene25653.t1 MTR_4g088210 49.169 301 139 3 17 304 78 377 3.60e-95 297
MS.gene25653.t1 MTR_4g088210 49.169 301 139 3 17 304 78 377 4.87e-95 297
MS.gene25653.t1 MTR_8g027440 49.186 307 148 6 2 306 61 361 6.59e-94 293
MS.gene25653.t1 MTR_2g093740 48.589 319 135 6 12 304 97 412 1.43e-93 293
MS.gene25653.t1 MTR_4g021580 49.838 309 146 6 2 307 51 353 1.60e-93 290
MS.gene25653.t1 MTR_4g021580 49.838 309 146 6 2 307 51 353 2.88e-93 291
MS.gene25653.t1 MTR_2g093740 48.589 319 135 6 12 304 97 412 9.51e-93 292
MS.gene25653.t1 MTR_2g093740 48.589 319 135 6 12 304 97 412 1.97e-92 293
MS.gene25653.t1 MTR_4g060460 48.026 304 146 4 12 304 95 397 1.86e-90 286
MS.gene25653.t1 MTR_4g060460 48.026 304 146 4 12 304 95 397 2.26e-90 286
MS.gene25653.t1 MTR_2g093740 51.481 270 124 3 43 307 5 272 4.81e-89 279
MS.gene25653.t1 MTR_2g093740 52.060 267 121 3 43 304 5 269 1.35e-88 279
MS.gene25653.t1 MTR_7g062540 46.254 307 158 5 2 306 46 347 1.07e-85 271
MS.gene25653.t1 MTR_2g093740 49.117 283 124 4 43 307 5 285 3.75e-85 269
MS.gene25653.t1 MTR_2g093740 49.643 280 121 4 43 304 5 282 8.13e-85 270
MS.gene25653.t1 MTR_2g094570 39.024 369 181 7 2 328 38 404 2.00e-76 247
MS.gene25653.t1 MTR_2g094570 39.024 369 181 7 2 328 38 404 4.16e-76 247
MS.gene25653.t1 MTR_2g094570 39.024 369 181 7 2 328 94 460 7.22e-76 247
MS.gene25653.t1 MTR_4g058930 39.714 350 162 5 22 322 56 405 1.54e-72 237
MS.gene25653.t1 MTR_1g094960 39.067 343 166 8 2 305 46 384 2.17e-72 235
MS.gene25653.t1 MTR_4g058930 39.714 350 162 5 22 322 56 405 2.98e-72 237
MS.gene25653.t1 MTR_1g064430 38.150 346 169 4 16 316 67 412 1.64e-69 229
MS.gene25653.t1 MTR_1g064430 38.150 346 169 4 16 316 80 425 1.74e-69 229
MS.gene25653.t1 MTR_8g446900 38.870 301 170 7 12 308 3 293 2.41e-69 221
MS.gene25653.t1 MTR_7g101280 34.853 373 179 9 14 328 69 435 4.92e-63 211
MS.gene25653.t1 MTR_0041s0110 34.853 373 179 9 14 328 69 435 4.92e-63 211
MS.gene25653.t1 MTR_3g073420 37.037 324 161 5 22 307 66 384 4.36e-62 207
MS.gene25653.t1 MTR_7g101275 38.686 274 160 4 14 282 30 300 2.90e-60 198
MS.gene25653.t1 MTR_3g064050 71.724 145 38 3 184 326 3 146 3.00e-58 198
MS.gene25653.t1 MTR_0041s0120 36.332 289 158 7 14 282 30 312 2.03e-53 181
MS.gene25653.t1 MTR_5g061220 34.062 320 164 7 13 303 66 367 4.38e-51 177
MS.gene25653.t1 MTR_5g082140 37.282 287 160 7 24 303 63 336 3.04e-50 173
MS.gene25653.t1 MTR_5g040880 35.473 296 173 5 22 313 60 341 6.43e-50 174
MS.gene25653.t1 MTR_2g024430 33.218 289 164 10 24 303 72 340 5.71e-43 155
MS.gene25653.t1 MTR_1g058210 30.479 292 187 6 16 303 59 338 2.15e-40 147
MS.gene25653.t1 MTR_2g006270 31.633 294 183 7 23 309 65 347 3.64e-40 146
MS.gene25653.t1 MTR_2g006380 31.773 299 169 10 23 309 72 347 1.10e-36 137
MS.gene25653.t1 MTR_5g074840 43.455 191 72 4 49 235 3 161 5.51e-36 128
MS.gene25653.t1 MTR_5g040740 30.909 275 152 6 24 283 63 314 1.51e-35 133
MS.gene25653.t1 MTR_5g032260 46.512 129 64 2 175 302 30 154 2.57e-31 122
MS.gene25653.t1 MTR_5g060630 27.812 320 195 8 19 305 58 374 2.04e-30 120
MS.gene25653.t1 MTR_5g460740 27.812 320 195 8 19 305 58 374 2.04e-30 120
MS.gene25653.t1 MTR_5g062970 36.076 158 91 3 19 170 81 234 8.53e-26 103
MS.gene25653.t1 MTR_5g460920 27.986 293 186 10 20 303 59 335 1.09e-25 106
MS.gene25653.t1 MTR_5g060780 27.835 291 189 9 20 303 59 335 1.39e-25 105
MS.gene25653.t1 MTR_5g061890 27.491 291 190 9 20 303 59 335 1.63e-24 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25653.t1 AT5G20730 89.329 328 35 0 1 328 54 381 0.0 595
MS.gene25653.t1 AT1G19220 88.110 328 39 0 1 328 53 380 0.0 593
MS.gene25653.t1 AT5G20730 89.058 329 35 1 1 328 54 382 0.0 591
MS.gene25653.t1 AT5G20730 89.058 329 35 1 1 328 54 382 0.0 590
MS.gene25653.t1 AT1G30330 71.687 332 88 6 1 328 53 382 7.90e-158 465
MS.gene25653.t1 AT1G30330 71.687 332 88 6 1 328 55 384 9.07e-158 465
MS.gene25653.t1 AT1G30330 71.687 332 88 6 1 328 55 384 9.07e-158 465
MS.gene25653.t1 AT1G19850 70.261 306 87 4 1 303 84 388 9.56e-151 446
MS.gene25653.t1 AT1G19850 70.261 306 87 4 1 303 73 377 1.11e-150 446
MS.gene25653.t1 AT5G37020 66.667 330 104 6 1 326 52 379 2.65e-148 436
MS.gene25653.t1 AT5G37020 66.667 330 104 6 1 326 52 379 1.47e-147 435
MS.gene25653.t1 AT5G37020 66.783 286 90 5 44 326 1 284 3.41e-126 377
MS.gene25653.t1 AT5G62000 53.271 321 149 1 2 322 93 412 4.86e-113 347
MS.gene25653.t1 AT5G62000 53.271 321 149 1 2 322 93 412 4.86e-113 347
MS.gene25653.t1 AT5G62000 53.271 321 149 1 2 322 93 412 4.86e-113 347
MS.gene25653.t1 AT5G62000 53.271 321 149 1 2 322 93 412 4.86e-113 347
MS.gene25653.t1 AT5G62000 53.271 321 149 1 2 322 93 412 6.46e-113 347
MS.gene25653.t1 AT1G59750 51.713 321 147 5 12 328 64 380 3.98e-108 329
MS.gene25653.t1 AT1G59750 51.713 321 147 5 12 328 64 380 4.28e-108 329
MS.gene25653.t1 AT1G59750 51.713 321 147 5 12 328 64 380 4.62e-108 329
MS.gene25653.t1 AT1G59750 51.713 321 147 5 12 328 64 380 4.62e-108 329
MS.gene25653.t1 AT5G60450 50.327 306 140 4 12 307 82 385 2.06e-98 307
MS.gene25653.t1 AT5G60450 50.327 306 140 4 12 307 106 409 3.73e-98 307
MS.gene25653.t1 AT4G23980 53.103 290 131 4 18 306 60 345 6.46e-96 297
MS.gene25653.t1 AT4G23980 53.103 290 131 4 18 306 60 345 6.64e-96 297
MS.gene25653.t1 AT2G46530 48.758 322 156 7 1 320 52 366 1.82e-95 295
MS.gene25653.t1 AT2G46530 48.758 322 156 7 1 320 73 387 2.36e-95 295
MS.gene25653.t1 AT2G33860 47.742 310 151 3 5 304 81 389 4.76e-94 291
MS.gene25653.t1 AT3G61830 47.937 315 158 5 1 314 5 314 3.63e-93 288
MS.gene25653.t1 AT3G61830 47.937 315 158 5 1 314 56 365 1.36e-92 288
MS.gene25653.t1 AT1G34410 43.464 306 166 3 1 306 57 355 1.28e-83 264
MS.gene25653.t1 AT2G33860 53.680 231 107 0 74 304 2 232 8.72e-83 258
MS.gene25653.t1 AT1G35240 42.444 311 172 3 1 311 57 360 2.66e-82 261
MS.gene25653.t1 AT2G46530 51.012 247 117 3 74 320 37 279 2.69e-82 258
MS.gene25653.t1 AT1G35540 42.904 303 166 3 1 303 57 352 9.86e-81 257
MS.gene25653.t1 AT1G34310 42.188 320 177 4 1 320 57 368 5.05e-80 254
MS.gene25653.t1 AT1G35520 42.904 303 166 3 1 303 57 352 7.40e-80 254
MS.gene25653.t1 AT1G34170 41.558 308 171 4 1 306 55 355 1.68e-77 246
MS.gene25653.t1 AT1G34390 42.812 320 173 5 1 320 57 366 1.23e-76 246
MS.gene25653.t1 AT2G28350 40.751 346 160 7 16 316 49 394 1.80e-72 236
MS.gene25653.t1 AT2G28350 40.751 346 160 7 16 316 49 394 2.52e-72 237
MS.gene25653.t1 AT4G30080 39.714 350 169 8 18 325 60 409 1.25e-71 234
MS.gene25653.t1 AT1G34170 37.987 308 156 4 1 306 55 329 5.37e-67 218
MS.gene25653.t1 AT1G34170 37.987 308 156 4 1 306 55 329 4.66e-66 217
MS.gene25653.t1 AT1G77850 33.989 356 189 8 11 326 52 401 1.95e-54 187
MS.gene25653.t1 AT1G77850 33.989 356 189 8 11 326 52 401 2.03e-54 187
MS.gene25653.t1 AT1G43950 40.462 173 96 3 1 173 57 222 1.28e-33 124

Find 74 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATACTGGCATCAGATTT+TGG 0.175907 8.2:-8054057 MS.gene25653:CDS
AGGGAACCTACTTTGTTTAC+AGG 0.231749 8.2:+8054212 None:intergenic
GCGTAGACCTCGTCAGTTTC+AGG 0.249723 8.2:+8054251 None:intergenic
GGATGGAAGATTGGGGTAAC+TGG 0.255295 8.2:+8054452 None:intergenic
GGATTGTAATATATAGTAAA+TGG 0.299159 8.2:+8053032 None:intergenic
TTCACAATGGCGCAATCTTC+AGG 0.308095 8.2:-8052733 MS.gene25653:intron
ACCTGGCATGCCAGGGTGGC+CGG 0.323931 8.2:+8052496 None:intergenic
GCCCAAGTAGAGTTTCGATT+TGG 0.326647 8.2:-8052583 MS.gene25653:CDS
CTCTTACTACCTGGCATGCC+AGG 0.340362 8.2:+8052488 None:intergenic
AGATACATGGGTACAGTTAC+AGG 0.345844 8.2:-8052789 MS.gene25653:CDS
ATCAACTTGGATGGAAGATT+GGG 0.367160 8.2:+8054444 None:intergenic
CCCAAGTAGAGTTTCGATTT+GGG 0.373867 8.2:-8052582 MS.gene25653:CDS
CTGGCATGCCAGGGTGGCCG+GGG 0.374556 8.2:+8052498 None:intergenic
GTAGTCAACAGATGTCTCTT+TGG 0.386649 8.2:+8053379 None:intergenic
CCTGGCATGCCAGGGTGGCC+GGG 0.389368 8.2:+8052497 None:intergenic
TGAGAAAATATTCCCACCTT+TGG 0.394909 8.2:-8053935 MS.gene25653:intron
AATCAACTTGGATGGAAGAT+TGG 0.404278 8.2:+8054443 None:intergenic
CCCGGCCACCCTGGCATGCC+AGG 0.405700 8.2:-8052497 None:intergenic
CTCCGAATTTGTTATTCCAT+TGG 0.413095 8.2:-8052898 MS.gene25653:CDS
TCCCAAATCGAAACTCTACT+TGG 0.415574 8.2:+8052581 None:intergenic
TCCAGTGACAGTATGCATAT+TGG 0.421107 8.2:-8053095 MS.gene25653:CDS
GTATGACAAGGACGCAATAC+TGG 0.422487 8.2:-8054070 MS.gene25653:CDS
GCCAATATGCATACTGTCAC+TGG 0.434302 8.2:+8053094 None:intergenic
CGGACTATTTACAGGTTGGA+TGG 0.440838 8.2:-8052628 MS.gene25653:intron
TTCAATGCAACCTCCCGCTC+AGG 0.444639 8.2:-8053796 MS.gene25653:CDS
GTACATGGCTTTGTTATACT+TGG 0.450701 8.2:+8052875 None:intergenic
GGCCAATGGAATAACAAATT+CGG 0.461554 8.2:+8052896 None:intergenic
AAGAGACATCTGTTGACTAC+TGG 0.463007 8.2:-8053376 MS.gene25653:CDS
ATGTTTGAAACAGAGGAGTC+TGG 0.469317 8.2:-8052819 MS.gene25653:CDS
TAGAATGATGTTTGAAACAG+AGG 0.475753 8.2:-8052826 MS.gene25653:CDS
AAGATCTGCATGACAATACA+TGG 0.486770 8.2:-8053761 MS.gene25653:CDS
AATAACAAATTCGGAGGGGC+TGG 0.488782 8.2:+8052905 None:intergenic
ATGTACACTCAAGTGTCTCT+TGG 0.490387 8.2:-8052858 MS.gene25653:CDS
AAGAGACACTTGAGTGTACA+TGG 0.494633 8.2:+8052860 None:intergenic
GCGTTTAGACATATTTATAG+AGG 0.495433 8.2:-8053738 MS.gene25653:intron
AAAAGAATTCGGTAGGTTGA+CGG 0.504207 8.2:+8054022 None:intergenic
CAAAACTTCCCCGGCCACCC+TGG 0.504342 8.2:-8052506 MS.gene25653:CDS
GTCTGGAGTACGTAGATACA+TGG 0.522979 8.2:-8052802 MS.gene25653:CDS
GATGGAAGATTGGGGTAACT+GGG 0.537088 8.2:+8054453 None:intergenic
GGACTATTTACAGGTTGGAT+GGG 0.539478 8.2:-8052627 MS.gene25653:intron
TTAGCAACAAGCTCCTGAGC+GGG 0.542449 8.2:+8053783 None:intergenic
GCTTTGTTATACTTGGCCAA+TGG 0.543342 8.2:+8052882 None:intergenic
GGAGGTGGACAAATATAGAA+AGG 0.544179 8.2:+8052539 None:intergenic
TTACTATATATTACAATCCA+AGG 0.545897 8.2:-8053028 MS.gene25653:intron
ATATAGAAAGGAGTCACTAC+TGG 0.547020 8.2:+8052551 None:intergenic
GTGAAGCATGCAAATCAACT+TGG 0.547588 8.2:+8054431 None:intergenic
GCTAGTGACACAAGCACTCA+TGG 0.549611 8.2:-8053985 MS.gene25653:CDS
TGTTGTATACACACCAAAGG+TGG 0.558659 8.2:+8053922 None:intergenic
AACACCTTCTTCTAGGCTTG+AGG 0.569429 8.2:-8053160 MS.gene25653:CDS
AGCATGCAAATCAACTTGGA+TGG 0.581262 8.2:+8054435 None:intergenic
CGCGCCTCAAGCCTAGAAGA+AGG 0.582337 8.2:+8053156 None:intergenic
GTCTCTTCTGCAGTATGACA+AGG 0.590870 8.2:-8054082 MS.gene25653:intron
GACATCTGTTGACTACTGGA+TGG 0.599027 8.2:-8053372 MS.gene25653:CDS
GGGGTAACTGGGTATGAAGT+CGG 0.599332 8.2:+8054464 None:intergenic
ATTACTGATGAAGAAAGCGC+TGG 0.602562 8.2:+8053119 None:intergenic
TCTTACTACCTGGCATGCCA+GGG 0.603552 8.2:+8052489 None:intergenic
GTTATTTGCAGCAGCATGAG+CGG 0.605180 8.2:+8053058 None:intergenic
CAATGGAATAACAAATTCGG+AGG 0.606134 8.2:+8052899 None:intergenic
GGATGGGATGAATCGACAGC+TGG 0.607046 8.2:-8052611 MS.gene25653:CDS
AGTTACAGGAATTAGTGACT+TGG 0.608495 8.2:-8052775 MS.gene25653:CDS
CCCAAATCGAAACTCTACTT+GGG 0.609411 8.2:+8052582 None:intergenic
GCAACAAGCTCCTGAGCGGG+AGG 0.612134 8.2:+8053786 None:intergenic
GTTGTATACACACCAAAGGT+GGG 0.618187 8.2:+8053923 None:intergenic
GTGACTTGGATCCTGTCCGA+TGG 0.622801 8.2:-8052761 MS.gene25653:CDS
GGAAAATGCACCTACATGCA+GGG 0.636686 8.2:+8054401 None:intergenic
AATGGAATAACAAATTCGGA+GGG 0.651060 8.2:+8052900 None:intergenic
TACTACCTGGCATGCCAGGG+TGG 0.655417 8.2:+8052492 None:intergenic
TCAACTTGGATGGAAGATTG+GGG 0.663761 8.2:+8054445 None:intergenic
GGCTGATCCTGAAACTGACG+AGG 0.667661 8.2:-8054258 MS.gene25653:CDS
AGATCTGCATGACAATACAT+GGG 0.692633 8.2:-8053760 MS.gene25653:CDS
TTTAGCAACAAGCTCCTGAG+CGG 0.693281 8.2:+8053782 None:intergenic
TCTGGAGTACGTAGATACAT+GGG 0.698431 8.2:-8052801 MS.gene25653:CDS
AGTGACACAAGCACTCATGG+TGG 0.722448 8.2:-8053982 MS.gene25653:CDS
ATGGAATAACAAATTCGGAG+GGG 0.734309 8.2:+8052901 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATCTTGTGT+TGG - chr8.2:8053736-8053755 MS.gene25653:intron 15.0%
!!! AAGAATATCTAAAATAAAAG+TGG + chr8.2:8053280-8053299 None:intergenic 15.0%
!! AATCTTCAGGTATTATTAAA+AGG - chr8.2:8054267-8054286 MS.gene25653:intron 20.0%
!! GATTATATATGAATGTAAGA+TGG + chr8.2:8053312-8053331 None:intergenic 20.0%
!! GGATTGTAATATATAGTAAA+TGG + chr8.2:8053958-8053977 None:intergenic 20.0%
!! GTGTATTATCATACATAATA+TGG - chr8.2:8053999-8054018 MS.gene25653:CDS 20.0%
!! TGAAAAAAAATTCTTCTGTA+TGG + chr8.2:8053473-8053492 None:intergenic 20.0%
!! TTACTATATATTACAATCCA+AGG - chr8.2:8053959-8053978 MS.gene25653:CDS 20.0%
!! TTTAGAAAGATAGAATGTAA+AGG + chr8.2:8053364-8053383 None:intergenic 20.0%
!!! ACAGCTTTATATTTGTATAT+TGG + chr8.2:8053528-8053547 None:intergenic 20.0%
!!! ATTGGTGTTTTTGTTTATTT+TGG - chr8.2:8052702-8052721 MS.gene25653:intron 20.0%
!!! GGTATATATTATCTTGTTAT+TGG - chr8.2:8053391-8053410 MS.gene25653:intron 20.0%
! AAATCCTATCAAAACACTTA+TGG + chr8.2:8053171-8053190 None:intergenic 25.0%
! ACAAGATAATATATACCTAC+AGG + chr8.2:8053388-8053407 None:intergenic 25.0%
! ATAGACAAGAAAAACCAAAA+GGG + chr8.2:8053143-8053162 None:intergenic 25.0%
! CAAGATAATATATACCTACA+GGG + chr8.2:8053387-8053406 None:intergenic 25.0%
!! ATTATTTTCGGACTATTTAC+AGG - chr8.2:8054351-8054370 MS.gene25653:intron 25.0%
!! CTTATTGGAGCAATTATTTT+CGG - chr8.2:8054339-8054358 MS.gene25653:intron 25.0%
!! TAGAGTTTTGCAAAAGAATT+CGG + chr8.2:8052979-8052998 None:intergenic 25.0%
!! TGATTCATTTTCGCAAAATT+TGG - chr8.2:8053101-8053120 MS.gene25653:CDS 25.0%
AACAAATTCTCAAACTGAAG+AGG + chr8.2:8052798-8052817 None:intergenic 30.0%
AATAAGCTCATGAAGAAGTT+CGG + chr8.2:8054325-8054344 None:intergenic 30.0%
AATGGAATAACAAATTCGGA+GGG + chr8.2:8054090-8054109 None:intergenic 30.0%
ACAAATTCTCAAACTGAAGA+GGG + chr8.2:8052797-8052816 None:intergenic 30.0%
AGAAAACGGTCAATTTAACA+GGG - chr8.2:8054059-8054078 MS.gene25653:CDS 30.0%
ATAGTTACTCATCATTTGCT+TGG + chr8.2:8052866-8052885 None:intergenic 30.0%
CATAGACAAGAAAAACCAAA+AGG + chr8.2:8053144-8053163 None:intergenic 30.0%
GCGTTTAGACATATTTATAG+AGG - chr8.2:8053249-8053268 MS.gene25653:intron 30.0%
TCCATCAACTTGTGATTTAT+TGG - chr8.2:8052684-8052703 MS.gene25653:intron 30.0%
TGCTGAAATTGATTGAACAT+TGG - chr8.2:8053574-8053593 MS.gene25653:intron 30.0%
TGGTCAAATCATTTACAAGA+CGG + chr8.2:8053508-8053527 None:intergenic 30.0%
! AAAAATTTGCTCAGAAACTG+TGG + chr8.2:8053781-8053800 None:intergenic 30.0%
! ACCAATAAATCACAAGTTGA+TGG + chr8.2:8052688-8052707 None:intergenic 30.0%
! GAGATTCTGTTCTTTTTATC+AGG - chr8.2:8053672-8053691 MS.gene25653:intron 30.0%
! TAGAATGATGTTTGAAACAG+AGG - chr8.2:8054161-8054180 MS.gene25653:intron 30.0%
! TTACAATCCAAGGTACTATT+TGG - chr8.2:8053969-8053988 MS.gene25653:CDS 30.0%
!!! GTTTTTGTTTATTTTGGAGC+AGG - chr8.2:8052708-8052727 MS.gene25653:intron 30.0%
AAGATCTGCATGACAATACA+TGG - chr8.2:8053226-8053245 MS.gene25653:intron 35.0%
AATCAACTTGGATGGAAGAT+TGG + chr8.2:8052547-8052566 None:intergenic 35.0%
AGATCTGCATGACAATACAT+GGG - chr8.2:8053227-8053246 MS.gene25653:intron 35.0%
AGTTACAGGAATTAGTGACT+TGG - chr8.2:8054212-8054231 MS.gene25653:intron 35.0%
ATACATGCCAAATAGTACCT+TGG + chr8.2:8053979-8053998 None:intergenic 35.0%
ATATAGAAAGGAGTCACTAC+TGG + chr8.2:8054439-8054458 None:intergenic 35.0%
ATCAACTTGGATGGAAGATT+GGG + chr8.2:8052546-8052565 None:intergenic 35.0%
ATGGAATAACAAATTCGGAG+GGG + chr8.2:8054089-8054108 None:intergenic 35.0%
CAATGGAATAACAAATTCGG+AGG + chr8.2:8054091-8054110 None:intergenic 35.0%
CAGAAAACGGTCAATTTAAC+AGG - chr8.2:8054058-8054077 MS.gene25653:CDS 35.0%
CTTCAACCTGTAAACAAAGT+AGG - chr8.2:8052769-8052788 MS.gene25653:CDS 35.0%
GAAAAACAACACCTTCTTCT+AGG - chr8.2:8053820-8053839 MS.gene25653:intron 35.0%
GAACTTCTTCATGAGCTTAT+TGG - chr8.2:8054324-8054343 MS.gene25653:intron 35.0%
GAATGTTGTATACACACCAA+AGG + chr8.2:8053071-8053090 None:intergenic 35.0%
GACTGTCACAAATATTTCGA+TGG + chr8.2:8054033-8054052 None:intergenic 35.0%
GAGGATGTCAAAGAAAAGTA+AGG + chr8.2:8052841-8052860 None:intergenic 35.0%
GGCCAATGGAATAACAAATT+CGG + chr8.2:8054094-8054113 None:intergenic 35.0%
TCCGATGGAAAAATTCACAA+TGG - chr8.2:8054241-8054260 MS.gene25653:CDS 35.0%
TCTATCTTTCTAAACCCTGT+AGG - chr8.2:8053370-8053389 MS.gene25653:CDS 35.0%
TGACAGTCTCTTACAGAAAA+CGG - chr8.2:8054045-8054064 MS.gene25653:CDS 35.0%
TGAGAAAATATTCCCACCTT+TGG - chr8.2:8053052-8053071 MS.gene25653:CDS 35.0%
! AAAAGAATTCGGTAGGTTGA+CGG + chr8.2:8052968-8052987 None:intergenic 35.0%
! AAGTTTTGCCTGAAAAATGG+AGG + chr8.2:8054469-8054488 None:intergenic 35.0%
! ATTGATTGAACATTGGCTAC+AGG - chr8.2:8053581-8053600 MS.gene25653:intron 35.0%
! GCCATTGTGAATTTTTCCAT+CGG + chr8.2:8054245-8054264 None:intergenic 35.0%
! GTTTTGCAAAAGAATTCGGT+AGG + chr8.2:8052975-8052994 None:intergenic 35.0%
! TTGATTGAACATTGGCTACA+GGG - chr8.2:8053582-8053601 MS.gene25653:intron 35.0%
! TTTTCGGACTATTTACAGGT+TGG - chr8.2:8054355-8054374 MS.gene25653:intron 35.0%
!! CTCCGAATTTGTTATTCCAT+TGG - chr8.2:8054089-8054108 MS.gene25653:intron 35.0%
!! GTACATGGCTTTGTTATACT+TGG + chr8.2:8054115-8054134 None:intergenic 35.0%
!!! CTGACCATAAGTGTTTTGAT+AGG - chr8.2:8053164-8053183 MS.gene25653:CDS 35.0%
AAGAGACACTTGAGTGTACA+TGG + chr8.2:8054130-8054149 None:intergenic 40.0%
ACTCATCATTTGCTTGGAAG+AGG + chr8.2:8052860-8052879 None:intergenic 40.0%
AGATACATGGGTACAGTTAC+AGG - chr8.2:8054198-8054217 MS.gene25653:intron 40.0%
AGCATGCAAATCAACTTGGA+TGG + chr8.2:8052555-8052574 None:intergenic 40.0%
ATGTACACTCAAGTGTCTCT+TGG - chr8.2:8054129-8054148 MS.gene25653:intron 40.0%
CCCAAATCGAAACTCTACTT+GGG + chr8.2:8054408-8054427 None:intergenic 40.0%
CCCAAGTAGAGTTTCGATTT+GGG - chr8.2:8054405-8054424 MS.gene25653:intron 40.0%
GGAGGTGGACAAATATAGAA+AGG + chr8.2:8054451-8054470 None:intergenic 40.0%
GTAGTCAACAGATGTCTCTT+TGG + chr8.2:8053611-8053630 None:intergenic 40.0%
GTGAAGCATGCAAATCAACT+TGG + chr8.2:8052559-8052578 None:intergenic 40.0%
GTTGTATACACACCAAAGGT+GGG + chr8.2:8053067-8053086 None:intergenic 40.0%
TCAACTTGGATGGAAGATTG+GGG + chr8.2:8052545-8052564 None:intergenic 40.0%
TCCAGTGACAGTATGCATAT+TGG - chr8.2:8053892-8053911 MS.gene25653:intron 40.0%
TCCCAAATCGAAACTCTACT+TGG + chr8.2:8054409-8054428 None:intergenic 40.0%
TGTTGTATACACACCAAAGG+TGG + chr8.2:8053068-8053087 None:intergenic 40.0%
! AAGAGACATCTGTTGACTAC+TGG - chr8.2:8053611-8053630 MS.gene25653:intron 40.0%
! AGCACAAAGAGACTTTTTGC+TGG - chr8.2:8053650-8053669 MS.gene25653:intron 40.0%
! AGGGAACCTACTTTGTTTAC+AGG + chr8.2:8052778-8052797 None:intergenic 40.0%
! ATGTTTGAAACAGAGGAGTC+TGG - chr8.2:8054168-8054187 MS.gene25653:intron 40.0%
! ATTACTGATGAAGAAAGCGC+TGG + chr8.2:8053871-8053890 None:intergenic 40.0%
! GCAATACTGGCATCAGATTT+TGG - chr8.2:8052930-8052949 MS.gene25653:intron 40.0%
! GCTTTGTTATACTTGGCCAA+TGG + chr8.2:8054108-8054127 None:intergenic 40.0%
! GGACTATTTACAGGTTGGAT+GGG - chr8.2:8054360-8054379 MS.gene25653:intron 40.0%
! GGGAAGTTTTGCCTGAAAAA+TGG + chr8.2:8054472-8054491 None:intergenic 40.0%
! TCTGGAGTACGTAGATACAT+GGG - chr8.2:8054186-8054205 MS.gene25653:intron 40.0%
! TGTGAATTTTTCCATCGGAC+AGG + chr8.2:8054240-8054259 None:intergenic 40.0%
! TTTTGCCTGAAAAATGGAGG+TGG + chr8.2:8054466-8054485 None:intergenic 40.0%
!! GAGCTTTATCTGATCCCTTT+TGG - chr8.2:8053126-8053145 MS.gene25653:CDS 40.0%
!! TTTGTCCACCTCCATTTTTC+AGG - chr8.2:8054458-8054477 MS.gene25653:CDS 40.0%
!!! TTTTTCAGGCAAAACTTCCC+CGG - chr8.2:8054472-8054491 MS.gene25653:CDS 40.0%
AACACCTTCTTCTAGGCTTG+AGG - chr8.2:8053827-8053846 MS.gene25653:intron 45.0%
AATAACAAATTCGGAGGGGC+TGG + chr8.2:8054085-8054104 None:intergenic 45.0%
CATAACAGCCACATCACAAG+CGG + chr8.2:8052610-8052629 None:intergenic 45.0%
GATGGAAGATTGGGGTAACT+GGG + chr8.2:8052537-8052556 None:intergenic 45.0%
GCCAATATGCATACTGTCAC+TGG + chr8.2:8053896-8053915 None:intergenic 45.0%
GCCCAAGTAGAGTTTCGATT+TGG - chr8.2:8054404-8054423 MS.gene25653:intron 45.0%
GGAAAATGCACCTACATGCA+GGG + chr8.2:8052589-8052608 None:intergenic 45.0%
GTATGACAAGGACGCAATAC+TGG - chr8.2:8052917-8052936 MS.gene25653:intron 45.0%
GTCTCTTCTGCAGTATGACA+AGG - chr8.2:8052905-8052924 MS.gene25653:CDS 45.0%
GTTATTTGCAGCAGCATGAG+CGG + chr8.2:8053932-8053951 None:intergenic 45.0%
TTCACAATGGCGCAATCTTC+AGG - chr8.2:8054254-8054273 MS.gene25653:CDS 45.0%
TTTAGCAACAAGCTCCTGAG+CGG + chr8.2:8053208-8053227 None:intergenic 45.0%
! ATTTTCTCAGCTGCTCTACG+AGG + chr8.2:8053041-8053060 None:intergenic 45.0%
! CGGACTATTTACAGGTTGGA+TGG - chr8.2:8054359-8054378 MS.gene25653:intron 45.0%
! GACATCTGTTGACTACTGGA+TGG - chr8.2:8053615-8053634 MS.gene25653:intron 45.0%
! GTCTGGAGTACGTAGATACA+TGG - chr8.2:8054185-8054204 MS.gene25653:intron 45.0%
! TGCATTTTCCGCTTGTGATG+TGG - chr8.2:8052599-8052618 MS.gene25653:CDS 45.0%
!!! GAAGTCGGTCTGTTTTTGCA+TGG + chr8.2:8052511-8052530 None:intergenic 45.0%
AGTGACACAAGCACTCATGG+TGG - chr8.2:8053005-8053024 MS.gene25653:intron 50.0%
CACAATGTTGCCCTGCATGT+AGG - chr8.2:8052576-8052595 MS.gene25653:CDS 50.0%
CGGAAAATGCACCTACATGC+AGG + chr8.2:8052590-8052609 None:intergenic 50.0%
GCTAGTGACACAAGCACTCA+TGG - chr8.2:8053002-8053021 MS.gene25653:intron 50.0%
GGATGGAAGATTGGGGTAAC+TGG + chr8.2:8052538-8052557 None:intergenic 50.0%
GGGGTAACTGGGTATGAAGT+CGG + chr8.2:8052526-8052545 None:intergenic 50.0%
TTAGCAACAAGCTCCTGAGC+GGG + chr8.2:8053207-8053226 None:intergenic 50.0%
GCGTAGACCTCGTCAGTTTC+AGG + chr8.2:8052739-8052758 None:intergenic 55.0%
GGATGGGATGAATCGACAGC+TGG - chr8.2:8054376-8054395 MS.gene25653:intron 55.0%
GGCTGATCCTGAAACTGACG+AGG - chr8.2:8052729-8052748 MS.gene25653:intron 55.0%
TTCAATGCAACCTCCCGCTC+AGG - chr8.2:8053191-8053210 MS.gene25653:intron 55.0%
! GTGACTTGGATCCTGTCCGA+TGG - chr8.2:8054226-8054245 MS.gene25653:CDS 55.0%
CGCGCCTCAAGCCTAGAAGA+AGG + chr8.2:8053834-8053853 None:intergenic 60.0%
CAAAACTTCCCCGGCCACCC+TGG - chr8.2:8054481-8054500 MS.gene25653:CDS 65.0%
GCAACAAGCTCCTGAGCGGG+AGG + chr8.2:8053204-8053223 None:intergenic 65.0%
CTGGCATGCCAGGGTGGCCG+GGG + chr8.2:8054492-8054511 None:intergenic 75.0%
! CCCGGCCACCCTGGCATGCC+AGG - chr8.2:8054490-8054509 MS.gene25653:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 8052498 8054511 8052498 ID=MS.gene25653
chr8.2 mRNA 8052498 8054511 8052498 ID=MS.gene25653.t1;Parent=MS.gene25653
chr8.2 exon 8054416 8054511 8054416 ID=MS.gene25653.t1.exon1;Parent=MS.gene25653.t1
chr8.2 CDS 8054416 8054511 8054416 ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2 exon 8054223 8054279 8054223 ID=MS.gene25653.t1.exon2;Parent=MS.gene25653.t1
chr8.2 CDS 8054223 8054279 8054223 ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2 exon 8053936 8054091 8053936 ID=MS.gene25653.t1.exon3;Parent=MS.gene25653.t1
chr8.2 CDS 8053936 8054091 8053936 ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2 exon 8053739 8053823 8053739 ID=MS.gene25653.t1.exon4;Parent=MS.gene25653.t1
chr8.2 CDS 8053739 8053823 8053739 ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2 exon 8053316 8053406 8053316 ID=MS.gene25653.t1.exon5;Parent=MS.gene25653.t1
chr8.2 CDS 8053316 8053406 8053316 ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2 exon 8053029 8053193 8053029 ID=MS.gene25653.t1.exon6;Parent=MS.gene25653.t1
chr8.2 CDS 8053029 8053193 8053029 ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2 exon 8052734 8052929 8052734 ID=MS.gene25653.t1.exon7;Parent=MS.gene25653.t1
chr8.2 CDS 8052734 8052929 8052734 ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
chr8.2 exon 8052498 8052636 8052498 ID=MS.gene25653.t1.exon8;Parent=MS.gene25653.t1
chr8.2 CDS 8052498 8052636 8052498 ID=cds.MS.gene25653.t1;Parent=MS.gene25653.t1
Gene Sequence

>MS.gene25653

GTTGCGGCTTCCATGCAAAAACAGACCGACTTCATACCCAGTTACCCCAATCTTCCATCCAAGTTGATTTGCATGCTTCACAATGTTGCCCTGCATGTAGGTGCATTTTCCGCTTGTGATGTGGCTGTTATGCAATAATGAAAACTTGTGAAGAAGAATTACTTATATACAGTTTATGATTTATATTCCATCAACTTGTGATTTATTGGTGTTTTTGTTTATTTTGGAGCAGGCTGATCCTGAAACTGACGAGGTCTACGCGCAAATGACACTTCAACCTGTAAACAAAGTAGGTTCCCTCTTCAGTTTGAGAATTTGTTATTGCGTCGCATGTTTAGTTCCTTACTTTTCTTTGACATCCTCTTCCAAGCAAATGATGAGTAACTATACAAAAGATTGTCTCATTTGTCTCTTCTGCAGTATGACAAGGACGCAATACTGGCATCAGATTTTGGTCTCAAACAAAACCGTCAACCTACCGAATTCTTTTGCAAAACTCTAACTGCTAGTGACACAAGCACTCATGGTGGATTTTCTGTTCCTCGTAGAGCAGCTGAGAAAATATTCCCACCTTTGGTGTGTATACAACATTCTCATATCTCTTGATTCATTTTCGCAAAATTTGGTAGAGCTTTATCTGATCCCTTTTGGTTTTTCTTGTCTATGCTGACCATAAGTGTTTTGATAGGATTTTTCAATGCAACCTCCCGCTCAGGAGCTTGTTGCTAAAGATCTGCATGACAATACATGGGCGTTTAGACATATTTATAGAGGTATTTCCACTTTTATTTTAGATATTCTTCATTCATTGCCATCTTACATTCATATATAATCATTAGTATATTGTATGCTTTATTGACGCACCTTTACATTCTATCTTTCTAAACCCTGTAGGTATATATTATCTTGTTATTGGTATGACAATGACATCACTTTTGTAAAAATGAAGCACATGCTAAAAATTGAGACTGTCCATACAGAAGAATTTTTTTTCAATAATCTTAGAACCGTCTTGTAAATGATTTGACCAATATACAAATATAAAGCTGTGTAATTTATGTGTTATGAAGTTTGAATGCTGAAATTGATTGAACATTGGCTACAGGGCAACCAAAGAGACATCTGTTGACTACTGGATGGAGTGTATTTATCAGCACAAAGAGACTTTTTGCTGGAGATTCTGTTCTTTTTATCAGGTAAGATATATATATATATATATATATATATATATATATATATATATATATATATCTTGTGTTGGAATTTCAGAGAATTTTTAACCACAGTTTCTGAGCAAATTTTTGTTGATTACTTTTAGAGATGAAAAACAACACCTTCTTCTAGGCTTGAGGCGCGCTAATAGACAGCAGCCAGCGCTTTCTTCATCAGTAATATCCAGTGACAGTATGCATATTGGCATTTTAGCTGCTGCCGCTCATGCTGCTGCAAATAACAGTCCATTTACTATATATTACAATCCAAGGTACTATTTGGCATGTATGTGTATTATCATACATAATATGGAATAGCATCCATCGAAATATTTGTGACAGTCTCTTACAGAAAACGGTCAATTTAACAGGGCCAGCCCCTCCGAATTTGTTATTCCATTGGCCAAGTATAACAAAGCCATGTACACTCAAGTGTCTCTTGGAATGAGATTTAGAATGATGTTTGAAACAGAGGAGTCTGGAGTACGTAGATACATGGGTACAGTTACAGGAATTAGTGACTTGGATCCTGTCCGATGGAAAAATTCACAATGGCGCAATCTTCAGGTATTATTAAAAGGTGTATTATAGTACTTGTTTTGATGTACTAATACCGAACTTCTTCATGAGCTTATTGGAGCAATTATTTTCGGACTATTTACAGGTTGGATGGGATGAATCGACAGCTGGTGAACGCCCAAGTAGAGTTTCGATTTGGGATATTGAACCAGTAGTGACTCCTTTCTATATTTGTCCACCTCCATTTTTCAGGCAAAACTTCCCCGGCCACCCTGGCATGCCAG
Protein sequence

>MS.gene25653.t1

VAASMQKQTDFIPSYPNLPSKLICMLHNVALHADPETDEVYAQMTLQPVNKYDKDAILASDFGLKQNRQPTEFFCKTLTASDTSTHGGFSVPRRAAEKIFPPLDFSMQPPAQELVAKDLHDNTWAFRHIYRGQPKRHLLTTGWSVFISTKRLFAGDSVLFIRDEKQHLLLGLRRANRQQPALSSSVISSDSMHIGILAAAAHAAANNSPFTIYYNPRASPSEFVIPLAKYNKAMYTQVSLGMRFRMMFETEESGVRRYMGTVTGISDLDPVRWKNSQWRNLQVGWDESTAGERPSRVSIWDIEPVVTPFYICPPPFFRQNFPGHPGMP