AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052358


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052358.t1 MTR_4g077300 100.000 210 0 0 1 210 59 268 1.63e-158 445
MS.gene052358.t1 MTR_4g077300 100.000 210 0 0 1 210 64 273 2.31e-158 445
MS.gene052358.t1 MTR_2g040010 85.714 210 30 0 1 210 47 256 6.61e-136 387
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052358.t1 AT5G57020 85.714 210 30 0 1 210 59 268 2.12e-139 397
MS.gene052358.t1 AT2G44175 53.333 60 28 0 2 61 37 96 2.01e-18 77.8

Find 66 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCGACATCAATTAGTTTCT+TGG 0.257794 4.4:-33390533 None:intergenic
TATAAAGGCAACCATCTTCT+TGG 0.307468 4.4:-33390281 None:intergenic
TGAAGTAGCCCGGAGGTTGC+AGG 0.313936 4.4:-33390225 None:intergenic
CCGTGATGAGGTTGTTAATA+TGG 0.329122 4.4:+33390330 MS.gene052358:CDS
CTGGTGTGACTGTTGGATTC+TGG 0.335007 4.4:-33390613 None:intergenic
CCTCATCACGGACTCGAATT+CGG 0.339678 4.4:-33390318 None:intergenic
GCGCTGGGCCCTGCAACCTC+CGG 0.353029 4.4:+33390216 MS.gene052358:CDS
AAGATGGTTGCCTTTATAAC+AGG 0.354888 4.4:+33390286 MS.gene052358:CDS
ATTCTCCAAGTGCACCCTTC+TGG 0.370742 4.4:-33390428 None:intergenic
CTCATCACGGACTCGAATTC+GGG 0.375743 4.4:-33390317 None:intergenic
TACTTCACAGCTTGGCATAT+TGG 0.377050 4.4:+33390241 MS.gene052358:CDS
CCGACATCAATTAGTTTCTT+GGG 0.382092 4.4:-33390532 None:intergenic
GGGTGCACTTGGAGAATATA+TGG 0.383844 4.4:+33390434 MS.gene052358:CDS
AAGATGATGAGAACATGTTT+AGG 0.387407 4.4:+33390170 MS.gene052358:CDS
CAGTAGCAACTTGCCAATAC+TGG 0.390437 4.4:+33390494 MS.gene052358:CDS
GGCAGGGATACCTGTTATAA+AGG 0.401328 4.4:-33390296 None:intergenic
GTTTCTCTCGGCTCGGTGCT+CGG 0.405888 4.4:+33390554 MS.gene052358:CDS
CCCCTTATCTGAGGTAAAAC+AGG 0.409550 4.4:+33390045 MS.gene052358:CDS
TGTGGGCAGTTCAAGGATAT+AGG 0.410449 4.4:+33389983 None:intergenic
CGCTGGGCCCTGCAACCTCC+GGG 0.413953 4.4:+33390217 MS.gene052358:CDS
TGTTAAAACTTCCAAGAAGA+TGG 0.425379 4.4:+33390270 MS.gene052358:CDS
ACCTTCCTAACCTCTATGAA+TGG 0.426427 4.4:+33390077 MS.gene052358:CDS
GTGGGCAGTTCAAGGATATA+GGG 0.445450 4.4:+33389984 None:intergenic
CAACATAATTATTAGCAAGA+AGG 0.456558 4.4:-33390147 None:intergenic
GGTGGTTCGATAGGACCTTC+AGG 0.457527 4.4:-33390019 None:intergenic
TAGAGGTTAGGAAGGTTGTA+TGG 0.462978 4.4:-33390070 None:intergenic
CTAAACCCTGGTGTGACTGT+TGG 0.470399 4.4:-33390620 None:intergenic
TTCGAAGGAATTTCTGCGCT+GGG 0.475576 4.4:+33390201 MS.gene052358:CDS
AACATAATTATTAGCAAGAA+GGG 0.476139 4.4:-33390146 None:intergenic
CAGTATTGGCAAGTTGCTAC+TGG 0.490516 4.4:-33390493 None:intergenic
TCAAAGATCTGTGCCAGTAT+TGG 0.491976 4.4:-33390507 None:intergenic
GTCACCTCTTTGATCATGAC+AGG 0.495174 4.4:-33390406 None:intergenic
GTAACCCATTCATAGAGGTT+AGG 0.503092 4.4:-33390082 None:intergenic
GGTGACCAGAAGGGTGCACT+TGG 0.504257 4.4:+33390423 MS.gene052358:CDS
CCTTCCTAACCTCTATGAAT+GGG 0.513056 4.4:+33390078 MS.gene052358:CDS
ATTCGAAGGAATTTCTGCGC+TGG 0.519044 4.4:+33390200 MS.gene052358:CDS
CTCCGGGCTACTTCACAGCT+TGG 0.520105 4.4:+33390233 MS.gene052358:CDS
TCACGGACTCGAATTCGGGC+AGG 0.527541 4.4:-33390313 None:intergenic
CCCATTCATAGAGGTTAGGA+AGG 0.528278 4.4:-33390078 None:intergenic
TGGCAAGTTGCTACTGGTGT+AGG 0.531802 4.4:-33390487 None:intergenic
CATGACAGGAGCAAGCCTCT+TGG 0.536419 4.4:-33390392 None:intergenic
TAATTGATGTCGGTTTCTCT+CGG 0.541266 4.4:+33390542 MS.gene052358:CDS
GAAGTAGCCCGGAGGTTGCA+GGG 0.543684 4.4:-33390224 None:intergenic
ATTTCCATCTTTCTAAACCC+TGG 0.548222 4.4:-33390632 None:intergenic
CACGGACTCGAATTCGGGCA+GGG 0.550332 4.4:-33390312 None:intergenic
AAGAATCACTCCTGCTGTGT+AGG 0.552278 4.4:-33390464 None:intergenic
GATGTCGGTTTCTCTCGGCT+CGG 0.552576 4.4:+33390547 MS.gene052358:CDS
TGCCAAGCTGTGAAGTAGCC+CGG 0.554188 4.4:-33390235 None:intergenic
CAGAATCCAACAGTCACACC+AGG 0.560206 4.4:+33390614 MS.gene052358:CDS
CCCAAGAAACTAATTGATGT+CGG 0.561835 4.4:+33390532 MS.gene052358:CDS
ATAGGACCTTCAGGCAAAGT+GGG 0.564234 4.4:-33390010 None:intergenic
TGGCAGGCTGCCTACACAGC+AGG 0.566798 4.4:+33390454 MS.gene052358:CDS
TGATCAAAGAGGTGACCAGA+AGG 0.575360 4.4:+33390413 MS.gene052358:CDS
CACAAGTAACCCATTCATAG+AGG 0.576099 4.4:-33390087 None:intergenic
GTTTAGGTTTAACTATTCGA+AGG 0.584434 4.4:+33390186 MS.gene052358:CDS
GATAGGACCTTCAGGCAAAG+TGG 0.591235 4.4:-33390011 None:intergenic
GCACTTGGAGAATATATGGC+AGG 0.591925 4.4:+33390438 MS.gene052358:CDS
GGGAACCCCACTTTGCCTGA+AGG 0.595352 4.4:+33390004 MS.gene052358:CDS
CCATATTAACAACCTCATCA+CGG 0.625479 4.4:-33390330 None:intergenic
ACAAGAAGCTTCGAACCAAG+AGG 0.628890 4.4:+33390377 MS.gene052358:CDS
GATCAAAGAGGTGACCAGAA+GGG 0.633175 4.4:+33390414 MS.gene052358:CDS
CCGAATTCGAGTCCGTGATG+AGG 0.640464 4.4:+33390318 MS.gene052358:CDS
TGCTCCTGTCATGATCAAAG+AGG 0.676350 4.4:+33390402 MS.gene052358:CDS
TAGGACCTTCAGGCAAAGTG+GGG 0.681811 4.4:-33390009 None:intergenic
AGAATCCAACAGTCACACCA+GGG 0.692322 4.4:+33390615 MS.gene052358:CDS
CAAGCTGTGAAGTAGCCCGG+AGG 0.761817 4.4:-33390232 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATAATTATTAGCAAGAA+GGG - chr4.4:33390149-33390168 None:intergenic 20.0%
! CAACATAATTATTAGCAAGA+AGG - chr4.4:33390150-33390169 None:intergenic 25.0%
AAGATGATGAGAACATGTTT+AGG + chr4.4:33390170-33390189 MS.gene052358:CDS 30.0%
TGTTAAAACTTCCAAGAAGA+TGG + chr4.4:33390270-33390289 MS.gene052358:CDS 30.0%
! GTTTAGGTTTAACTATTCGA+AGG + chr4.4:33390186-33390205 MS.gene052358:CDS 30.0%
AAGATGGTTGCCTTTATAAC+AGG + chr4.4:33390286-33390305 MS.gene052358:CDS 35.0%
CCATATTAACAACCTCATCA+CGG - chr4.4:33390333-33390352 None:intergenic 35.0%
CCCAAGAAACTAATTGATGT+CGG + chr4.4:33390532-33390551 MS.gene052358:CDS 35.0%
TATAAAGGCAACCATCTTCT+TGG - chr4.4:33390284-33390303 None:intergenic 35.0%
! ACCGACATCAATTAGTTTCT+TGG - chr4.4:33390536-33390555 None:intergenic 35.0%
! CCGACATCAATTAGTTTCTT+GGG - chr4.4:33390535-33390554 None:intergenic 35.0%
! TCCTGTTTTACCTCAGATAA+GGG - chr4.4:33390049-33390068 None:intergenic 35.0%
! TTCCTGTTTTACCTCAGATA+AGG - chr4.4:33390050-33390069 None:intergenic 35.0%
!! TAATTGATGTCGGTTTCTCT+CGG + chr4.4:33390542-33390561 MS.gene052358:CDS 35.0%
ACCTTCCTAACCTCTATGAA+TGG + chr4.4:33390077-33390096 MS.gene052358:CDS 40.0%
CACAAGTAACCCATTCATAG+AGG - chr4.4:33390090-33390109 None:intergenic 40.0%
CCGTGATGAGGTTGTTAATA+TGG + chr4.4:33390330-33390349 MS.gene052358:CDS 40.0%
CCTTCCTAACCTCTATGAAT+GGG + chr4.4:33390078-33390097 MS.gene052358:CDS 40.0%
GTAACCCATTCATAGAGGTT+AGG - chr4.4:33390085-33390104 None:intergenic 40.0%
TACTTCACAGCTTGGCATAT+TGG + chr4.4:33390241-33390260 MS.gene052358:CDS 40.0%
TAGAGGTTAGGAAGGTTGTA+TGG - chr4.4:33390073-33390092 None:intergenic 40.0%
TCAAAGATCTGTGCCAGTAT+TGG - chr4.4:33390510-33390529 None:intergenic 40.0%
! CCTGTTTTACCTCAGATAAG+GGG - chr4.4:33390048-33390067 None:intergenic 40.0%
! CTGTTTTACCTCAGATAAGG+GGG - chr4.4:33390047-33390066 None:intergenic 40.0%
AAGAATCACTCCTGCTGTGT+AGG - chr4.4:33390467-33390486 None:intergenic 45.0%
ACAAGAAGCTTCGAACCAAG+AGG + chr4.4:33390377-33390396 MS.gene052358:CDS 45.0%
AGAATCCAACAGTCACACCA+GGG + chr4.4:33390615-33390634 MS.gene052358:CDS 45.0%
ATAGGACCTTCAGGCAAAGT+GGG - chr4.4:33390013-33390032 None:intergenic 45.0%
ATTCGAAGGAATTTCTGCGC+TGG + chr4.4:33390200-33390219 MS.gene052358:CDS 45.0%
CAGTAGCAACTTGCCAATAC+TGG + chr4.4:33390494-33390513 MS.gene052358:CDS 45.0%
CCCATTCATAGAGGTTAGGA+AGG - chr4.4:33390081-33390100 None:intergenic 45.0%
GATCAAAGAGGTGACCAGAA+GGG + chr4.4:33390414-33390433 MS.gene052358:CDS 45.0%
GCACTTGGAGAATATATGGC+AGG + chr4.4:33390438-33390457 MS.gene052358:CDS 45.0%
GGCAGGGATACCTGTTATAA+AGG - chr4.4:33390299-33390318 None:intergenic 45.0%
GGGTGCACTTGGAGAATATA+TGG + chr4.4:33390434-33390453 MS.gene052358:CDS 45.0%
GTCACCTCTTTGATCATGAC+AGG - chr4.4:33390409-33390428 None:intergenic 45.0%
TGATCAAAGAGGTGACCAGA+AGG + chr4.4:33390413-33390432 MS.gene052358:CDS 45.0%
TGCTCCTGTCATGATCAAAG+AGG + chr4.4:33390402-33390421 MS.gene052358:CDS 45.0%
TTCGAAGGAATTTCTGCGCT+GGG + chr4.4:33390201-33390220 MS.gene052358:CDS 45.0%
TTTACCTCAGATAAGGGGGT+TGG - chr4.4:33390043-33390062 None:intergenic 45.0%
! CCCCTTATCTGAGGTAAAAC+AGG + chr4.4:33390045-33390064 MS.gene052358:CDS 45.0%
!! CAGTATTGGCAAGTTGCTAC+TGG - chr4.4:33390496-33390515 None:intergenic 45.0%
ATTCTCCAAGTGCACCCTTC+TGG - chr4.4:33390431-33390450 None:intergenic 50.0%
CAGAATCCAACAGTCACACC+AGG + chr4.4:33390614-33390633 MS.gene052358:CDS 50.0%
CCTCATCACGGACTCGAATT+CGG - chr4.4:33390321-33390340 None:intergenic 50.0%
CTAAACCCTGGTGTGACTGT+TGG - chr4.4:33390623-33390642 None:intergenic 50.0%
CTCATCACGGACTCGAATTC+GGG - chr4.4:33390320-33390339 None:intergenic 50.0%
CTGGTGTGACTGTTGGATTC+TGG - chr4.4:33390616-33390635 None:intergenic 50.0%
GATAGGACCTTCAGGCAAAG+TGG - chr4.4:33390014-33390033 None:intergenic 50.0%
TAGGACCTTCAGGCAAAGTG+GGG - chr4.4:33390012-33390031 None:intergenic 50.0%
TGGCAAGTTGCTACTGGTGT+AGG - chr4.4:33390490-33390509 None:intergenic 50.0%
ACCACCAACCCCCTTATCTG+AGG + chr4.4:33390036-33390055 MS.gene052358:CDS 55.0%
ACCTCAGATAAGGGGGTTGG+TGG - chr4.4:33390040-33390059 None:intergenic 55.0%
CATGACAGGAGCAAGCCTCT+TGG - chr4.4:33390395-33390414 None:intergenic 55.0%
CCGAATTCGAGTCCGTGATG+AGG + chr4.4:33390318-33390337 MS.gene052358:CDS 55.0%
GGTGGTTCGATAGGACCTTC+AGG - chr4.4:33390022-33390041 None:intergenic 55.0%
TGCCAAGCTGTGAAGTAGCC+CGG - chr4.4:33390238-33390257 None:intergenic 55.0%
!! AAGGGGGTTGGTGGTTCGAT+AGG - chr4.4:33390031-33390050 None:intergenic 55.0%
!! GATGTCGGTTTCTCTCGGCT+CGG + chr4.4:33390547-33390566 MS.gene052358:CDS 55.0%
CAAGCTGTGAAGTAGCCCGG+AGG - chr4.4:33390235-33390254 None:intergenic 60.0%
CACGGACTCGAATTCGGGCA+GGG - chr4.4:33390315-33390334 None:intergenic 60.0%
CTCCGGGCTACTTCACAGCT+TGG + chr4.4:33390233-33390252 MS.gene052358:CDS 60.0%
GAAGTAGCCCGGAGGTTGCA+GGG - chr4.4:33390227-33390246 None:intergenic 60.0%
GGGAACCCCACTTTGCCTGA+AGG + chr4.4:33390004-33390023 MS.gene052358:CDS 60.0%
TCACGGACTCGAATTCGGGC+AGG - chr4.4:33390316-33390335 None:intergenic 60.0%
TGAAGTAGCCCGGAGGTTGC+AGG - chr4.4:33390228-33390247 None:intergenic 60.0%
! GGTGACCAGAAGGGTGCACT+TGG + chr4.4:33390423-33390442 MS.gene052358:CDS 60.0%
!! GTTTCTCTCGGCTCGGTGCT+CGG + chr4.4:33390554-33390573 MS.gene052358:CDS 60.0%
TGGCAGGCTGCCTACACAGC+AGG + chr4.4:33390454-33390473 MS.gene052358:CDS 65.0%
CGCTGGGCCCTGCAACCTCC+GGG + chr4.4:33390217-33390236 MS.gene052358:CDS 75.0%
GCGCTGGGCCCTGCAACCTC+CGG + chr4.4:33390216-33390235 MS.gene052358:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 33389992 33390642 33389992 ID=MS.gene052358
chr4.4 mRNA 33389992 33390642 33389992 ID=MS.gene052358.t1;Parent=MS.gene052358
chr4.4 exon 33389992 33390642 33389992 ID=MS.gene052358.t1.exon1;Parent=MS.gene052358.t1
chr4.4 CDS 33389992 33390642 33389992 ID=cds.MS.gene052358.t1;Parent=MS.gene052358.t1
Gene Sequence

>MS.gene052358

TTCAAGGATATAGGGAACCCCACTTTGCCTGAAGGTCCTATCGAACCACCAACCCCCTTATCTGAGGTAAAACAGGAACCATACAACCTTCCTAACCTCTATGAATGGGTTACTTGTGACATTCATGACGAGCAGATGTGTGATGAGATATATACCCTTCTTGCTAATAATTATGTTGAAGATGATGAGAACATGTTTAGGTTTAACTATTCGAAGGAATTTCTGCGCTGGGCCCTGCAACCTCCGGGCTACTTCACAGCTTGGCATATTGGTGTTCGTGTTAAAACTTCCAAGAAGATGGTTGCCTTTATAACAGGTATCCCTGCCCGAATTCGAGTCCGTGATGAGGTTGTTAATATGGCAGAAATTAACTTTTTGTGTGTTCACAAGAAGCTTCGAACCAAGAGGCTTGCTCCTGTCATGATCAAAGAGGTGACCAGAAGGGTGCACTTGGAGAATATATGGCAGGCTGCCTACACAGCAGGAGTGATTCTTCCTACACCAGTAGCAACTTGCCAATACTGGCACAGATCTTTGAATCCCAAGAAACTAATTGATGTCGGTTTCTCTCGGCTCGGTGCTCGGATGACAATGAGTAGAACTATCAAACTTTACAAGCTACCAGAATCCAACAGTCACACCAGGGTTTAG

Protein sequence

>MS.gene052358.t1

FKDIGNPTLPEGPIEPPTPLSEVKQEPYNLPNLYEWVTCDIHDEQMCDEIYTLLANNYVEDDENMFRFNYSKEFLRWALQPPGYFTAWHIGVRVKTSKKMVAFITGIPARIRVRDEVVNMAEINFLCVHKKLRTKRLAPVMIKEVTRRVHLENIWQAAYTAGVILPTPVATCQYWHRSLNPKKLIDVGFSRLGARMTMSRTIKLYKLPESNSHTRV