AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25869

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25869.t1 MTR_3g479460 96.186 839 32 0 1 839 65 903 0.0 1680
MS.gene25869.t1 MTR_3g079450 79.548 841 169 3 1 839 63 902 0.0 1421
MS.gene25869.t1 MTR_3g079420 75.148 845 196 6 7 839 23 865 0.0 1283
MS.gene25869.t1 MTR_4g066170 63.227 843 294 7 8 839 69 906 0.0 1138
MS.gene25869.t1 MTR_3g079450 78.608 589 123 3 1 587 63 650 0.0 978
MS.gene25869.t1 MTR_8g020970 47.924 843 414 11 14 838 91 926 0.0 816
MS.gene25869.t1 MTR_8g080230 47.580 847 413 14 14 839 80 916 0.0 809
MS.gene25869.t1 MTR_1g083020 43.743 855 455 12 5 839 10 858 0.0 701
MS.gene25869.t1 MTR_1g104650 46.046 784 395 12 74 839 83 856 0.0 694
MS.gene25869.t1 MTR_4g088810 43.195 845 427 16 14 838 83 894 0.0 679
MS.gene25869.t1 MTR_8g018420 44.019 836 430 16 24 839 39 856 0.0 677
MS.gene25869.t1 MTR_8g018730 43.234 835 438 14 26 839 45 864 0.0 676
MS.gene25869.t1 MTR_8g018735 42.736 826 440 14 30 839 57 865 0.0 676
MS.gene25869.t1 MTR_2g099570 43.621 823 435 14 32 839 53 861 0.0 674
MS.gene25869.t1 MTR_8g018570 43.458 856 433 17 18 839 32 870 0.0 674
MS.gene25869.t1 MTR_8g018510 43.438 861 438 16 2 839 35 869 0.0 671
MS.gene25869.t1 MTR_2g099560 43.305 829 439 14 27 839 50 863 0.0 664
MS.gene25869.t1 MTR_7g113410 43.584 826 435 15 35 839 52 867 0.0 662
MS.gene25869.t1 MTR_8g018520 45.349 774 398 13 77 839 109 868 0.0 661
MS.gene25869.t1 MTR_5g024020 42.099 848 450 15 14 839 39 867 0.0 660
MS.gene25869.t1 MTR_8g018690 44.792 768 406 10 77 839 111 865 0.0 660
MS.gene25869.t1 MTR_8g018430 45.349 774 398 12 77 839 102 861 0.0 655
MS.gene25869.t1 MTR_8g018730 42.428 832 419 14 26 839 45 834 0.0 652
MS.gene25869.t1 MTR_8g020990 44.031 779 411 11 72 839 104 868 0.0 648
MS.gene25869.t1 MTR_8g018450 42.566 834 433 15 18 839 29 828 0.0 648
MS.gene25869.t1 MTR_8g018550 44.675 770 401 11 81 839 113 868 0.0 633
MS.gene25869.t1 MTR_8g018690 43.490 768 396 11 77 839 111 845 0.0 632
MS.gene25869.t1 MTR_3g081130 41.127 834 457 15 26 839 39 858 0.0 624
MS.gene25869.t1 MTR_5g024020 40.566 848 427 15 14 839 39 831 0.0 624
MS.gene25869.t1 MTR_3g081130 43.043 769 421 12 77 839 29 786 0.0 619
MS.gene25869.t1 MTR_8g018590 43.906 763 383 16 91 839 92 823 0.0 601
MS.gene25869.t1 MTR_8g018620 42.707 761 380 11 84 839 98 807 0.0 568
MS.gene25869.t1 MTR_8g018650 42.050 761 385 11 84 839 98 807 0.0 555
MS.gene25869.t1 MTR_8g011340 39.406 774 425 18 48 794 66 822 0.0 541
MS.gene25869.t1 MTR_8g020970 46.102 590 298 8 14 587 91 676 0.0 539
MS.gene25869.t1 MTR_8g018730 44.013 593 311 10 257 839 3 584 7.02e-171 507
MS.gene25869.t1 MTR_8g018735 41.856 571 301 13 33 587 60 615 3.53e-142 435
MS.gene25869.t1 MTR_6g452500 37.459 614 349 17 224 822 12 605 9.56e-125 389
MS.gene25869.t1 MTR_8g021020 45.025 402 207 5 445 839 37 431 2.49e-113 352
MS.gene25869.t1 MTR_7g012030 97.479 119 3 0 489 607 85 203 2.88e-76 246
MS.gene25869.t1 MTR_6g046330 88.462 130 4 1 489 607 75 204 4.26e-70 234
MS.gene25869.t1 MTR_8g463810 49.569 232 53 2 392 623 190 357 3.55e-63 218
MS.gene25869.t1 MTR_2g067130 84.483 58 9 0 1 58 1 58 6.23e-24 99.4
MS.gene25869.t1 MTR_4g088805 46.154 91 48 1 749 838 1 91 8.11e-18 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25869.t1 AT3G45140 55.253 809 344 7 36 839 101 896 0.0 937
MS.gene25869.t1 AT3G45140 56.955 762 315 6 83 839 1 754 0.0 916
MS.gene25869.t1 AT1G72520 47.754 846 411 16 14 839 92 926 0.0 803
MS.gene25869.t1 AT1G17420 46.963 856 425 12 4 839 73 919 0.0 799
MS.gene25869.t1 AT1G67560 46.856 811 411 12 41 839 115 917 0.0 757
MS.gene25869.t1 AT1G55020 47.694 824 399 15 35 839 49 859 0.0 723
MS.gene25869.t1 AT3G22400 44.217 830 425 14 39 839 66 886 0.0 690

Find 176 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGCAATGTTAAATTAT+TGG 0.149707 3.2:+69830511 None:intergenic
ATTCTGGTGGACCATAAATT+TGG 0.173285 3.2:+69830068 None:intergenic
CTAGCAATTGTTGGTCTATT+AGG 0.203211 3.2:+69828780 None:intergenic
CAATTATTGCCTTGACTAAT+TGG 0.209625 3.2:+69830214 None:intergenic
TTTGAGTTACCAGCAGATTT+TGG 0.210007 3.2:-69831532 MS.gene25869:CDS
TTGACTAATTGGATGCTATT+TGG 0.210653 3.2:+69830225 None:intergenic
GAGCTCACTAGACCTATTAT+TGG 0.251180 3.2:-69829748 MS.gene25869:CDS
TGACTAATTGGATGCTATTT+GGG 0.253957 3.2:+69830226 None:intergenic
CTTCTGATTCTACCAATCTT+TGG 0.255433 3.2:-69829690 MS.gene25869:CDS
ATAGCATTTGAAAGGTTTAA+TGG 0.262850 3.2:-69828549 MS.gene25869:CDS
TCAAATGCTATCTTTATATC+TGG 0.267837 3.2:+69828561 None:intergenic
AACTTTGCACAATATGCTTA+TGG 0.278802 3.2:-69828813 MS.gene25869:CDS
AAATTAGAATCCCAAATTTA+TGG 0.285172 3.2:-69830079 MS.gene25869:CDS
TCAACCTGGGAATGGCCTTT+TGG 0.293605 3.2:+69830492 None:intergenic
CAAGCCTGAGCAAACACTTT+TGG 0.295167 3.2:-69828703 MS.gene25869:CDS
TTTAGGCCATGTTGTGCATT+AGG 0.307604 3.2:+69829110 None:intergenic
TATCAAAGCGCAAAAGGTTT+TGG 0.308278 3.2:+69830450 None:intergenic
GTGAAGAAAACTCTCTTTGT+AGG 0.309146 3.2:+69831376 None:intergenic
GATTCAATGATGCTTGGATT+TGG 0.313188 3.2:+69828993 None:intergenic
CCAATGATGGTCTTCTCATA+TGG 0.313736 3.2:-69829061 MS.gene25869:CDS
ATTCTACCAATCTTTGGCTT+TGG 0.318489 3.2:-69829684 MS.gene25869:CDS
TGGAAATGTGGGCGCTGTTT+TGG 0.329432 3.2:-69831512 MS.gene25869:CDS
TTCAAATGAAGGAACAAATT+CGG 0.330717 3.2:-69831626 MS.gene25869:intron
CTAATAGAATTAAGAGGAAA+TGG 0.330995 3.2:-69831139 MS.gene25869:CDS
CTTTGTAGGTGTATCATGCT+TGG 0.333114 3.2:+69831390 None:intergenic
TGTGGTTTGTCACCAATAAT+AGG 0.333497 3.2:+69829736 None:intergenic
GTGACTCCAGGTCCTGAAAA+TGG 0.335649 3.2:+69828426 None:intergenic
CATTTGTGATTGCAACAAAT+AGG 0.340981 3.2:-69829487 MS.gene25869:CDS
CTACCAATCTTTGGCTTTGG+AGG 0.343171 3.2:-69829681 MS.gene25869:CDS
AAACTCAAAAGACCTGTTCT+TGG 0.345034 3.2:-69831037 MS.gene25869:CDS
GAGATTCTTATTAGTTCATA+TGG 0.346520 3.2:-69829383 MS.gene25869:CDS
GCTTTGGCAATTTGATTTGC+AGG 0.346703 3.2:-69829294 MS.gene25869:CDS
GTCATCGAGTCGCAGATAAT+TGG 0.351690 3.2:-69830028 MS.gene25869:CDS
ATTCTCACTGATATAAATCT+TGG 0.352672 3.2:-69830602 MS.gene25869:CDS
TACTAAATAGATATGCTATA+TGG 0.354697 3.2:+69828390 None:intergenic
TTCTCCCATTCTTCCTTTGT+AGG 0.357198 3.2:+69828735 None:intergenic
CTCATCAAGAATATTGTTAC+TGG 0.359156 3.2:-69831949 MS.gene25869:CDS
AATGGTTTCATTAGCTCATA+AGG 0.360058 3.2:+69828444 None:intergenic
TAGCATTTGAAAGGTTTAAT+GGG 0.363606 3.2:-69828548 MS.gene25869:CDS
CAAATACTAATTTGAAGAAT+AGG 0.370666 3.2:-69828485 MS.gene25869:CDS
GTGCAGGAGACAAATTCTTT+TGG 0.372279 3.2:-69830293 MS.gene25869:intron
AATATTGTTACTGGTATTGT+TGG 0.378392 3.2:-69831940 MS.gene25869:CDS
AGTCTTCAATTGCTAGCTTT+AGG 0.379422 3.2:+69829093 None:intergenic
TTCTGGTGGACCATAAATTT+GGG 0.383171 3.2:+69830069 None:intergenic
AAACAATTTCTTCTCTTGAA+TGG 0.386249 3.2:+69829900 None:intergenic
TCTCTCGTGATAGCTGATTC+TGG 0.393236 3.2:+69830052 None:intergenic
TGGCTTCAATGTGCCTTGTT+TGG 0.396948 3.2:+69829780 None:intergenic
CCTTGGATACGGATATTGTT+TGG 0.398283 3.2:+69831009 None:intergenic
TTTGTAGGTGTATCATGCTT+GGG 0.400836 3.2:+69831391 None:intergenic
CTAAATAACATAGTCCTTGA+CGG 0.401370 3.2:-69831460 MS.gene25869:CDS
CTGTGGCATATGATAAGCTT+TGG 0.401553 3.2:-69829310 MS.gene25869:CDS
GATGAGCTAATAGAATTAAG+AGG 0.404269 3.2:-69831145 MS.gene25869:CDS
TTGAGTTTAATGTTGGTGTC+AGG 0.407624 3.2:+69831052 None:intergenic
TCCATCTTAGTTGTCGATCA+TGG 0.412724 3.2:-69831420 MS.gene25869:CDS
TTTGCTAGGGAGACTTTGGC+AGG 0.413977 3.2:-69830255 MS.gene25869:CDS
TATGATGTCTACAATGATCT+TGG 0.416200 3.2:-69831079 MS.gene25869:CDS
TCCCTCACTTTGCTTACATA+AGG 0.416450 3.2:+69829850 None:intergenic
TTTCATTTACCATCATCTCT+TGG 0.418695 3.2:-69830536 MS.gene25869:CDS
TGATCAGATTCAATGATGCT+TGG 0.424472 3.2:+69828987 None:intergenic
ACCAGTGGCTTATAAGTTCA+TGG 0.425133 3.2:+69829620 None:intergenic
TCTCCCATTCTTCCTTTGTA+GGG 0.428559 3.2:+69828736 None:intergenic
CTAAGCTTTCTTAATCCACT+TGG 0.430925 3.2:+69831172 None:intergenic
CTATTTGTTGCAATCACAAA+TGG 0.433779 3.2:+69829488 None:intergenic
TTCACCAGATGAAGAGTACT+TGG 0.441500 3.2:-69828616 MS.gene25869:CDS
AGAAGACCATCATTGGCAAA+AGG 0.448419 3.2:+69829068 None:intergenic
CATGTTCTTACTCATGATAC+TGG 0.449029 3.2:-69829646 MS.gene25869:CDS
GTATGATAATAAGTTCAAAA+TGG 0.450817 3.2:+69828641 None:intergenic
CTGAAGAATACTACACTGTA+TGG 0.452168 3.2:-69829826 MS.gene25869:CDS
GGTCGAAAGCATTCTAATAC+AGG 0.461714 3.2:-69830974 MS.gene25869:intron
TAATTTAACATTGCTCCAAA+AGG 0.464271 3.2:-69830507 MS.gene25869:CDS
TACCGGTGGAGAAGATCATT+TGG 0.465498 3.2:+69829266 None:intergenic
AAGGTTTAATGGGAGATTGA+AGG 0.467037 3.2:-69828538 MS.gene25869:CDS
GGAATTTGCTAGGGAGACTT+TGG 0.467432 3.2:-69830259 MS.gene25869:CDS
TGTTTGGTGCCACCAAGAAC+AGG 0.467750 3.2:+69831025 None:intergenic
ACTCCCTTTCCAGTGACTCC+AGG 0.469105 3.2:+69828414 None:intergenic
ATGTTGTTTCTAGCAATTGT+TGG 0.470322 3.2:+69828771 None:intergenic
TGTCCCAAGTACTCTTCATC+TGG 0.470767 3.2:+69828612 None:intergenic
ATGGGATGCAATTAAACAAT+GGG 0.473897 3.2:-69829042 MS.gene25869:CDS
CCATATGAGAAGACCATCAT+TGG 0.474130 3.2:+69829061 None:intergenic
AAGACCCTACAAAGGAAGAA+TGG 0.474435 3.2:-69828740 MS.gene25869:CDS
CACTCCAAAAGTGTTTGCTC+AGG 0.477405 3.2:+69828699 None:intergenic
CTAAGATGGACAGGTCCATC+AGG 0.482847 3.2:+69831433 None:intergenic
TCCTTTGTAGGGTCTTCTGT+AGG 0.484655 3.2:+69828747 None:intergenic
AGACCCTACAAAGGAAGAAT+GGG 0.490464 3.2:-69828739 MS.gene25869:CDS
TTGAAGAATAGGAATGGAGC+TGG 0.490528 3.2:-69828474 MS.gene25869:CDS
ATAGACGAACTTTACAAAGA+AGG 0.490922 3.2:-69830560 MS.gene25869:CDS
CTGGAGTATCAAAGCGCAAA+AGG 0.494625 3.2:+69830444 None:intergenic
CATCATCACCACTATAGCTT+GGG 0.495297 3.2:-69828862 MS.gene25869:CDS
TAATTGGATGCTATTTGGGT+TGG 0.495555 3.2:+69830230 None:intergenic
GGACCTGGAGTCACTGGAAA+GGG 0.496747 3.2:-69828417 MS.gene25869:CDS
ATCATATGAAAAGAGAGATT+CGG 0.497530 3.2:-69830732 MS.gene25869:CDS
TATCCAAGGCGTTGTCGTAC+CGG 0.500621 3.2:-69830995 MS.gene25869:CDS
AGAGAATGAGGTGCAATATG+AGG 0.502465 3.2:-69831560 MS.gene25869:CDS
GTTCAAAATGGTCATCACTA+AGG 0.504252 3.2:+69828653 None:intergenic
GACAACTCATTGAACCATCA+TGG 0.507354 3.2:-69828593 MS.gene25869:CDS
TGGACAGAAGTTCGAACAAA+AGG 0.507956 3.2:-69828948 MS.gene25869:CDS
ATTAGCAACTTTAATCAACC+TGG 0.508311 3.2:+69830478 None:intergenic
AACTTTAATCAACCTGGGAA+TGG 0.508384 3.2:+69830484 None:intergenic
CGACCGGTACGACAACGCCT+TGG 0.509007 3.2:+69830992 None:intergenic
ACATCATCACCACTATAGCT+TGG 0.510325 3.2:-69828863 MS.gene25869:CDS
AACACAACCACGGCGTCCTC+AGG 0.513052 3.2:-69830656 MS.gene25869:CDS
AAGCCTCCAAAGCCAAAGAT+TGG 0.513343 3.2:+69829678 None:intergenic
AGTACTCTTCATCTGGTGAA+TGG 0.520631 3.2:+69828619 None:intergenic
TGCTCCAAAAGGCCATTCCC+AGG 0.522858 3.2:-69830496 MS.gene25869:CDS
GAAGACCATCATTGGCAAAA+GGG 0.524945 3.2:+69829069 None:intergenic
CCCACATTTCCAAAATCTGC+TGG 0.525769 3.2:+69831523 None:intergenic
TCCATGATCGACAACTAAGA+TGG 0.527836 3.2:+69831419 None:intergenic
ACTAATTTGAAGAATAGGAA+TGG 0.529661 3.2:-69828480 MS.gene25869:CDS
CTCAAAAGACCTGTTCTTGG+TGG 0.530119 3.2:-69831034 MS.gene25869:CDS
CTGTTCTTCCTCACCAAACA+AGG 0.534647 3.2:-69829793 MS.gene25869:CDS
GTACGACAACGCCTTGGATA+CGG 0.536846 3.2:+69830998 None:intergenic
CATAAGGTAGATATAAATCA+TGG 0.538440 3.2:+69829866 None:intergenic
TATCAGATGAGGAATTTGCT+AGG 0.542131 3.2:-69830269 MS.gene25869:CDS
GGGAGATTGAAGGAAATTGA+AGG 0.543222 3.2:-69828528 MS.gene25869:CDS
CAATGATGGTCTTCTCATAT+GGG 0.543920 3.2:-69829060 MS.gene25869:CDS
AGATGCTACCCAAGCTATAG+TGG 0.544008 3.2:+69828854 None:intergenic
GGATGCAGTAACTTGTAGAT+TGG 0.544887 3.2:+69829446 None:intergenic
AAGGTTGCTTGAATTTGTGA+AGG 0.545614 3.2:+69828672 None:intergenic
ATATAAAGATAGCATTTGAA+AGG 0.547239 3.2:-69828557 MS.gene25869:CDS
AATCCACTTGGTGTTTGTGA+TGG 0.549751 3.2:+69831184 None:intergenic
GTGAAGATTTGTTTCCATTG+TGG 0.552925 3.2:+69829718 None:intergenic
GAATTAAGAGGAAATGGAGA+AGG 0.558426 3.2:-69831133 MS.gene25869:CDS
TGAAGAATAGGAATGGAGCT+GGG 0.560809 3.2:-69828473 MS.gene25869:CDS
TGCTAGGGAGACTTTGGCAG+GGG 0.560834 3.2:-69830253 MS.gene25869:CDS
CTTTGGCAATTTGATTTGCA+GGG 0.562249 3.2:-69829293 MS.gene25869:CDS
ATCAGTGAGAATTGTGTCCA+AGG 0.562472 3.2:+69830613 None:intergenic
TTATTGGATCCAAGAGATGA+TGG 0.563781 3.2:+69830527 None:intergenic
TGGTTATACCACAGTTGAAG+AGG 0.565567 3.2:-69830008 MS.gene25869:intron
GATCGACAACTAAGATGGAC+AGG 0.574532 3.2:+69831424 None:intergenic
ACCATGAACTTATAAGCCAC+TGG 0.574627 3.2:-69829621 MS.gene25869:intron
ACAACTCATTGAACCATCAT+GGG 0.577441 3.2:-69828592 MS.gene25869:CDS
TATGGGATGCAATTAAACAA+TGG 0.577797 3.2:-69829043 MS.gene25869:CDS
AGGTTGCTTGAATTTGTGAA+GGG 0.579161 3.2:+69828673 None:intergenic
AGGACCTGGAGTCACTGGAA+AGG 0.579892 3.2:-69828418 MS.gene25869:CDS
CCAAACAATATCCGTATCCA+AGG 0.581077 3.2:-69831009 MS.gene25869:CDS
TGTATTAGAATGCTTTCGAC+CGG 0.581752 3.2:+69830976 None:intergenic
TCTGGATTCTCAGCCCATGA+TGG 0.583202 3.2:+69828579 None:intergenic
TCCAAGTGCATTTATCTGCA+AGG 0.586289 3.2:+69829409 None:intergenic
ACCTTGCAGATAAATGCACT+TGG 0.589225 3.2:-69829410 MS.gene25869:CDS
AGATTGGTAGAATCAGAAGC+AGG 0.591256 3.2:+69829694 None:intergenic
CACATTATGAGATTTAGACT+CGG 0.593059 3.2:+69832008 None:intergenic
TTCAATGGAATTGAGTTCTG+TGG 0.597142 3.2:-69829327 MS.gene25869:CDS
TGTCTATGTTCCTCGCGATG+AGG 0.598647 3.2:-69830708 MS.gene25869:CDS
ACAAGGCACATTGAAGCCAC+TGG 0.598697 3.2:-69829776 MS.gene25869:CDS
ATCAGATGAGGAATTTGCTA+GGG 0.601114 3.2:-69830268 MS.gene25869:CDS
TACCTTATGTAAGCAAAGTG+AGG 0.601124 3.2:-69829852 MS.gene25869:CDS
TTAGCAACTTTAATCAACCT+GGG 0.603980 3.2:+69830479 None:intergenic
TACCAAATGATCTTCTCCAC+CGG 0.604794 3.2:-69829268 MS.gene25869:intron
ACCTTATGTAAGCAAAGTGA+GGG 0.605287 3.2:-69829851 MS.gene25869:CDS
TTACCATCACAAACACCAAG+TGG 0.605695 3.2:-69831187 MS.gene25869:CDS
TCAATGTGCCTTGTTTGGTG+AGG 0.613172 3.2:+69829785 None:intergenic
GCTTAGTACCTCTTCAACTG+TGG 0.616160 3.2:+69830000 None:intergenic
CTGATCAAGAGCTTCAAGCA+TGG 0.620687 3.2:-69828971 MS.gene25869:CDS
TAACTTGTAGATTGGATGCA+TGG 0.623713 3.2:+69829454 None:intergenic
TTTGCACAATATGCTTATGG+AGG 0.625959 3.2:-69828810 MS.gene25869:CDS
AAATAGCATCCAATTAGTCA+AGG 0.636861 3.2:-69830223 MS.gene25869:intron
ATCAAGAGCTTCAAGCATGG+TGG 0.642027 3.2:-69828968 MS.gene25869:CDS
GATTCTAATTTACTTCTCAG+AGG 0.642337 3.2:+69830091 None:intergenic
GTCCATCAGGAAAACCGTCA+AGG 0.645251 3.2:+69831446 None:intergenic
TCAGCAAAAGCCTCATCGCG+AGG 0.648871 3.2:+69830698 None:intergenic
TGCAAGGTATATCTGAGATG+TGG 0.649204 3.2:+69829425 None:intergenic
GAAGTTCGAACAAAAGGTCA+CGG 0.654694 3.2:-69828942 MS.gene25869:CDS
CTCGTGATAGCTGATTCTGG+TGG 0.654841 3.2:+69830055 None:intergenic
TTGCTAGGGAGACTTTGGCA+GGG 0.658261 3.2:-69830254 MS.gene25869:CDS
CTAGTGAGCTCAATGACCAG+TGG 0.660322 3.2:+69829760 None:intergenic
TCACCAGATGAAGAGTACTT+GGG 0.668317 3.2:-69828615 MS.gene25869:CDS
ACTGTGAGGCAAAGTGATGG+TGG 0.687744 3.2:-69831973 MS.gene25869:CDS
GAAGATCCTAATGCACAACA+TGG 0.692109 3.2:-69829116 MS.gene25869:CDS
GCGACAAATCAGAAGAACCA+TGG 0.695121 3.2:-69828920 MS.gene25869:CDS
GTGACTGTGAGGCAAAGTGA+TGG 0.699606 3.2:-69831976 MS.gene25869:CDS
GCCTACAGAAGACCCTACAA+AGG 0.701209 3.2:-69828748 MS.gene25869:CDS
TTATTGGTGACAAACCACAA+TGG 0.706609 3.2:-69829732 MS.gene25869:CDS
TAGTGGTGATGATGTCAATG+AGG 0.710309 3.2:+69828871 None:intergenic
GATACAATTCAACACAACCA+CGG 0.729752 3.2:-69830666 MS.gene25869:CDS
ACAAATCAGAAGAACCATGG+TGG 0.737932 3.2:-69828917 MS.gene25869:CDS
TGAAAGCTTTAGTGACTGTG+AGG 0.746797 3.2:-69831987 MS.gene25869:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAGTAATATAATAAATATT+TGG + chr3.2:69830073-69830092 None:intergenic 10.0%
!!! ATTTAAGTTTTATATATACA+TGG - chr3.2:69829121-69829140 MS.gene25869:CDS 10.0%
!! AATAGCATACAAATTAAATT+TGG + chr3.2:69829178-69829197 None:intergenic 15.0%
!! ATATGTTATAAATTAGAGAA+AGG + chr3.2:69829535-69829554 None:intergenic 15.0%
!! ATTAAAACATAAATAAACCA+TGG + chr3.2:69828621-69828640 None:intergenic 15.0%
!! CAATAATTGTTACAAATATT+TGG - chr3.2:69830219-69830238 MS.gene25869:intron 15.0%
!! TCAAAATTATTTATATATGC+TGG + chr3.2:69829493-69829512 None:intergenic 15.0%
!!! TTAATTTGAATATTATATCC+AGG - chr3.2:69830496-69830515 MS.gene25869:CDS 15.0%
!! AAATTAGAATCCCAAATTTA+TGG - chr3.2:69830340-69830359 MS.gene25869:intron 20.0%
!! AATTTACTAGTAACAATGAA+TGG + chr3.2:69828695-69828714 None:intergenic 20.0%
!! ATATAAAGATAGCATTTGAA+AGG - chr3.2:69831862-69831881 MS.gene25869:intron 20.0%
!! ATTTGAATAAAATAAACGTG+AGG + chr3.2:69828780-69828799 None:intergenic 20.0%
!! CAAATACTAATTTGAAGAAT+AGG - chr3.2:69831934-69831953 MS.gene25869:CDS 20.0%
!! GTATGATAATAAGTTCAAAA+TGG + chr3.2:69831781-69831800 None:intergenic 20.0%
!! TAGAATCTTAGATTAAGTTA+TGG - chr3.2:69830462-69830481 MS.gene25869:CDS 20.0%
!! TTATATCATCTATGCTAATT+AGG - chr3.2:69830256-69830275 MS.gene25869:CDS 20.0%
!! TTGTGTTATATATATATGCT+AGG + chr3.2:69829643-69829662 None:intergenic 20.0%
!!! CGTTTATTTTATTCAAATGA+AGG - chr3.2:69828782-69828801 MS.gene25869:CDS 20.0%
!!! CTTTACTAAAAAGTATTCAA+TGG - chr3.2:69831077-69831096 MS.gene25869:CDS 20.0%
!!! TATATGTTGTTGATTTTTAC+AGG - chr3.2:69830884-69830903 MS.gene25869:intron 20.0%
!!! TTTTACAAGTTATTTGAGTT+GGG - chr3.2:69830093-69830112 MS.gene25869:CDS 20.0%
! AAACAATTTCTTCTCTTGAA+TGG + chr3.2:69830522-69830541 None:intergenic 25.0%
! AACATAAATAAACCATGGTT+TGG + chr3.2:69828616-69828635 None:intergenic 25.0%
! AATATTGTTACTGGTATTGT+TGG - chr3.2:69828479-69828498 MS.gene25869:CDS 25.0%
! ACTAATTTGAAGAATAGGAA+TGG - chr3.2:69831939-69831958 MS.gene25869:CDS 25.0%
! ATAGCATTTGAAAGGTTTAA+TGG - chr3.2:69831870-69831889 MS.gene25869:intron 25.0%
! ATCATATGAAAAGAGAGATT+CGG - chr3.2:69829687-69829706 MS.gene25869:CDS 25.0%
! ATTCTCACTGATATAAATCT+TGG - chr3.2:69829817-69829836 MS.gene25869:CDS 25.0%
! CATAAGGTAGATATAAATCA+TGG + chr3.2:69830556-69830575 None:intergenic 25.0%
! CTAATAGAATTAAGAGGAAA+TGG - chr3.2:69829280-69829299 MS.gene25869:CDS 25.0%
! GAGATTCTTATTAGTTCATA+TGG - chr3.2:69831036-69831055 MS.gene25869:CDS 25.0%
! GCAATAAAATGAAACTTACT+TGG + chr3.2:69828548-69828567 None:intergenic 25.0%
! GTGTATTCATTATGTTATGT+AGG - chr3.2:69830300-69830319 MS.gene25869:intron 25.0%
! TAATTTAACATTGCTCCAAA+AGG - chr3.2:69829912-69829931 MS.gene25869:intron 25.0%
! TAGCATTTGAAAGGTTTAAT+GGG - chr3.2:69831871-69831890 MS.gene25869:intron 25.0%
! TATGTATGTACACAAAACTT+TGG - chr3.2:69828665-69828684 MS.gene25869:CDS 25.0%
! TCAAATGCTATCTTTATATC+TGG + chr3.2:69831861-69831880 None:intergenic 25.0%
! TGATATAGGAGAGAAATTTA+GGG + chr3.2:69831239-69831258 None:intergenic 25.0%
! TGCATCAGAAAATTTGATAT+AGG + chr3.2:69831253-69831272 None:intergenic 25.0%
! TTCAAATGAAGGAACAAATT+CGG - chr3.2:69828793-69828812 MS.gene25869:CDS 25.0%
! TTCATTATGTTATGTAGGAA+TGG - chr3.2:69830305-69830324 MS.gene25869:intron 25.0%
! TTGATATAGGAGAGAAATTT+AGG + chr3.2:69831240-69831259 None:intergenic 25.0%
! TTGGAGCAATGTTAAATTAT+TGG + chr3.2:69829911-69829930 None:intergenic 25.0%
! TTTGGTCATGTAAATAAAGT+TGG + chr3.2:69829160-69829179 None:intergenic 25.0%
!! GAAATCTTTTGTTCTTTTCT+TGG - chr3.2:69830278-69830297 MS.gene25869:CDS 25.0%
!! GTTTTACAAGTTATTTGAGT+TGG - chr3.2:69830092-69830111 MS.gene25869:CDS 25.0%
!! TCTTTTTGTGTTTTCAGATT+TGG + chr3.2:69831528-69831547 None:intergenic 25.0%
!!! AAAAACAATTTTGGAATCCT+TGG - chr3.2:69829789-69829808 MS.gene25869:CDS 25.0%
!!! TTGTTGATTTTTACAGGTTA+AGG - chr3.2:69830890-69830909 MS.gene25869:intron 25.0%
AAATAGCATCCAATTAGTCA+AGG - chr3.2:69830196-69830215 MS.gene25869:intron 30.0%
AACTGAGAAAAAAGAGAATG+AGG - chr3.2:69828847-69828866 MS.gene25869:CDS 30.0%
AACTTTGCACAATATGCTTA+TGG - chr3.2:69831606-69831625 MS.gene25869:CDS 30.0%
AATGGTTTCATTAGCTCATA+AGG + chr3.2:69831978-69831997 None:intergenic 30.0%
ATAAAATAAACGTGAGGTCT+AGG + chr3.2:69828774-69828793 None:intergenic 30.0%
ATAGACGAACTTTACAAAGA+AGG - chr3.2:69829859-69829878 MS.gene25869:CDS 30.0%
ATATGAACACAATTCTTCGA+AGG - chr3.2:69828745-69828764 MS.gene25869:CDS 30.0%
ATGATACGTATAGACTTTAC+CGG + chr3.2:69831173-69831192 None:intergenic 30.0%
ATGGGATGCAATTAAACAAT+GGG - chr3.2:69831377-69831396 MS.gene25869:CDS 30.0%
ATTAGCAACTTTAATCAACC+TGG + chr3.2:69829944-69829963 None:intergenic 30.0%
CAATTATTGCCTTGACTAAT+TGG + chr3.2:69830208-69830227 None:intergenic 30.0%
CACATTATGAGATTTAGACT+CGG + chr3.2:69828414-69828433 None:intergenic 30.0%
CATTTGTGATTGCAACAAAT+AGG - chr3.2:69830932-69830951 MS.gene25869:intron 30.0%
CTAAATAACATAGTCCTTGA+CGG - chr3.2:69828959-69828978 MS.gene25869:CDS 30.0%
CTCATCAAGAATATTGTTAC+TGG - chr3.2:69828470-69828489 MS.gene25869:CDS 30.0%
GATGAGCTAATAGAATTAAG+AGG - chr3.2:69829274-69829293 MS.gene25869:CDS 30.0%
GATTCTAATTTACTTCTCAG+AGG + chr3.2:69830331-69830350 None:intergenic 30.0%
GTCATGTAAATAAAGTTGGA+TGG + chr3.2:69829156-69829175 None:intergenic 30.0%
TATGATGTCTACAATGATCT+TGG - chr3.2:69829340-69829359 MS.gene25869:CDS 30.0%
TATGGGATGCAATTAAACAA+TGG - chr3.2:69831376-69831395 MS.gene25869:CDS 30.0%
TCGGAGAAAAAGACAATAAA+AGG - chr3.2:69828812-69828831 MS.gene25869:CDS 30.0%
TTAGCAACTTTAATCAACCT+GGG + chr3.2:69829943-69829962 None:intergenic 30.0%
TTGACTAATTGGATGCTATT+TGG + chr3.2:69830197-69830216 None:intergenic 30.0%
TTTCATTTACCATCATCTCT+TGG - chr3.2:69829883-69829902 MS.gene25869:CDS 30.0%
! ATCATGTTTTACCTTGATTC+TGG - chr3.2:69830834-69830853 MS.gene25869:intron 30.0%
! CTATTTGTTGCAATCACAAA+TGG + chr3.2:69830934-69830953 None:intergenic 30.0%
! CTCAGGAGTAAAAACAATTT+TGG - chr3.2:69829780-69829799 MS.gene25869:CDS 30.0%
! GAATCTCTCTTTTCATATGA+TGG + chr3.2:69829688-69829707 None:intergenic 30.0%
! TGACTAATTGGATGCTATTT+GGG + chr3.2:69830196-69830215 None:intergenic 30.0%
!! ATGTTGTTTCTAGCAATTGT+TGG + chr3.2:69831651-69831670 None:intergenic 30.0%
!! GCAAATTGTTGTTCTTTTAG+AGG - chr3.2:69831270-69831289 MS.gene25869:intron 30.0%
!! TGGTAACAAACACTTGTTTT+TGG - chr3.2:69828499-69828518 MS.gene25869:CDS 30.0%
!! TTGTTGTTCTTTTAGAGGAA+TGG - chr3.2:69831275-69831294 MS.gene25869:intron 30.0%
!!! AGGTCTTTTGAGTTTAATGT+TGG + chr3.2:69829377-69829396 None:intergenic 30.0%
!!! CAAAATTGTTTTTACTCCTG+AGG + chr3.2:69829782-69829801 None:intergenic 30.0%
AAACTCAAAAGACCTGTTCT+TGG - chr3.2:69829382-69829401 MS.gene25869:CDS 35.0%
AACTTTAATCAACCTGGGAA+TGG + chr3.2:69829938-69829957 None:intergenic 35.0%
AAGGTTGCTTGAATTTGTGA+AGG + chr3.2:69831750-69831769 None:intergenic 35.0%
AAGGTTTAATGGGAGATTGA+AGG - chr3.2:69831881-69831900 MS.gene25869:intron 35.0%
ACAACTCATTGAACCATCAT+GGG - chr3.2:69831827-69831846 MS.gene25869:intron 35.0%
ACCTTATGTAAGCAAAGTGA+GGG - chr3.2:69830568-69830587 MS.gene25869:CDS 35.0%
AGGTTGCTTGAATTTGTGAA+GGG + chr3.2:69831749-69831768 None:intergenic 35.0%
CAATGATGGTCTTCTCATAT+GGG - chr3.2:69831359-69831378 MS.gene25869:intron 35.0%
CATGTTCTTACTCATGATAC+TGG - chr3.2:69830773-69830792 MS.gene25869:intron 35.0%
CTAAGCTTTCTTAATCCACT+TGG + chr3.2:69829250-69829269 None:intergenic 35.0%
CTGAAGAATACTACACTGTA+TGG - chr3.2:69830593-69830612 MS.gene25869:CDS 35.0%
CTTCTGATTCTACCAATCTT+TGG - chr3.2:69830729-69830748 MS.gene25869:CDS 35.0%
GAATTAAGAGGAAATGGAGA+AGG - chr3.2:69829286-69829305 MS.gene25869:CDS 35.0%
GATACAATTCAACACAACCA+CGG - chr3.2:69829753-69829772 MS.gene25869:CDS 35.0%
GATGTTACTGTAAAGGACAA+TGG + chr3.2:69828594-69828613 None:intergenic 35.0%
GATTCAATGATGCTTGGATT+TGG + chr3.2:69831429-69831448 None:intergenic 35.0%
GTGAAGAAAACTCTCTTTGT+AGG + chr3.2:69829046-69829065 None:intergenic 35.0%
GTTCAAAATGGTCATCACTA+AGG + chr3.2:69831769-69831788 None:intergenic 35.0%
TACCTTATGTAAGCAAAGTG+AGG - chr3.2:69830567-69830586 MS.gene25869:CDS 35.0%
TATAGGAGAGAAATTTAGGG+AGG + chr3.2:69831236-69831255 None:intergenic 35.0%
TGATCAGATTCAATGATGCT+TGG + chr3.2:69831435-69831454 None:intergenic 35.0%
TGTATTAGAATGCTTTCGAC+CGG + chr3.2:69829446-69829465 None:intergenic 35.0%
TTACAGTAACATCCAAACCA+TGG - chr3.2:69828601-69828620 MS.gene25869:CDS 35.0%
TTATTGGATCCAAGAGATGA+TGG + chr3.2:69829895-69829914 None:intergenic 35.0%
TTCAATGGAATTGAGTTCTG+TGG - chr3.2:69831092-69831111 MS.gene25869:CDS 35.0%
TTTGCACAATATGCTTATGG+AGG - chr3.2:69831609-69831628 MS.gene25869:CDS 35.0%
TTTGTAGGTGTATCATGCTT+GGG + chr3.2:69829031-69829050 None:intergenic 35.0%
! ATTCTACCAATCTTTGGCTT+TGG - chr3.2:69830735-69830754 MS.gene25869:CDS 35.0%
! ATTCTGGTGGACCATAAATT+TGG + chr3.2:69830354-69830373 None:intergenic 35.0%
! CTAGCAATTGTTGGTCTATT+AGG + chr3.2:69831642-69831661 None:intergenic 35.0%
! GAGAGTTTTCTTCACTAACA+AGG - chr3.2:69829051-69829070 MS.gene25869:CDS 35.0%
! GAGCTAATGAAACCATTTTC+AGG - chr3.2:69831981-69832000 MS.gene25869:CDS 35.0%
! GTGAAGATTTGTTTCCATTG+TGG + chr3.2:69830704-69830723 None:intergenic 35.0%
! TAACTTGTAGATTGGATGCA+TGG + chr3.2:69830968-69830987 None:intergenic 35.0%
! TAATTGGATGCTATTTGGGT+TGG + chr3.2:69830192-69830211 None:intergenic 35.0%
! TATCAAAGCGCAAAAGGTTT+TGG + chr3.2:69829972-69829991 None:intergenic 35.0%
! TATCAGATGAGGAATTTGCT+AGG - chr3.2:69830150-69830169 MS.gene25869:intron 35.0%
! TGGTTTGGATGTTACTGTAA+AGG + chr3.2:69828601-69828620 None:intergenic 35.0%
! TGTGGTTTGTCACCAATAAT+AGG + chr3.2:69830686-69830705 None:intergenic 35.0%
! TTATTGGTGACAAACCACAA+TGG - chr3.2:69830687-69830706 MS.gene25869:CDS 35.0%
! TTCTGGTGGACCATAAATTT+GGG + chr3.2:69830353-69830372 None:intergenic 35.0%
! TTGAGTTTAATGTTGGTGTC+AGG + chr3.2:69829370-69829389 None:intergenic 35.0%
!! AGTCTTCAATTGCTAGCTTT+AGG + chr3.2:69831329-69831348 None:intergenic 35.0%
!! ATCAGATGAGGAATTTGCTA+GGG - chr3.2:69830151-69830170 MS.gene25869:intron 35.0%
!! ATTCTTTTGGCTATCAGATG+AGG - chr3.2:69830139-69830158 MS.gene25869:intron 35.0%
!! CTTTGGCAATTTGATTTGCA+GGG - chr3.2:69831126-69831145 MS.gene25869:CDS 35.0%
!! TTTGAGTTACCAGCAGATTT+TGG - chr3.2:69828887-69828906 MS.gene25869:CDS 35.0%
AAGACCCTACAAAGGAAGAA+TGG - chr3.2:69831679-69831698 MS.gene25869:intron 40.0%
ACAAATCAGAAGAACCATGG+TGG - chr3.2:69831502-69831521 MS.gene25869:CDS 40.0%
ACATCATCACCACTATAGCT+TGG - chr3.2:69831556-69831575 MS.gene25869:CDS 40.0%
ACCAGTGGCTTATAAGTTCA+TGG + chr3.2:69830802-69830821 None:intergenic 40.0%
ACCATGAACTTATAAGCCAC+TGG - chr3.2:69830798-69830817 MS.gene25869:intron 40.0%
ACCTTGCAGATAAATGCACT+TGG - chr3.2:69831009-69831028 MS.gene25869:CDS 40.0%
AGAAGACCATCATTGGCAAA+AGG + chr3.2:69831354-69831373 None:intergenic 40.0%
AGACCCTACAAAGGAAGAAT+GGG - chr3.2:69831680-69831699 MS.gene25869:intron 40.0%
AGAGAATGAGGTGCAATATG+AGG - chr3.2:69828859-69828878 MS.gene25869:CDS 40.0%
AGATTGGTAGAATCAGAAGC+AGG + chr3.2:69830728-69830747 None:intergenic 40.0%
AGTACTCTTCATCTGGTGAA+TGG + chr3.2:69831803-69831822 None:intergenic 40.0%
ATACGTATAGACTTTACCGG+TGG + chr3.2:69831170-69831189 None:intergenic 40.0%
ATCAGTGAGAATTGTGTCCA+AGG + chr3.2:69829809-69829828 None:intergenic 40.0%
ATTTCTTCTCTTGAATGGCC+TGG + chr3.2:69830517-69830536 None:intergenic 40.0%
CATCATCACCACTATAGCTT+GGG - chr3.2:69831557-69831576 MS.gene25869:CDS 40.0%
CCAAACAATATCCGTATCCA+AGG - chr3.2:69829410-69829429 MS.gene25869:CDS 40.0%
CCAATGATGGTCTTCTCATA+TGG - chr3.2:69831358-69831377 MS.gene25869:intron 40.0%
CCATATGAGAAGACCATCAT+TGG + chr3.2:69831361-69831380 None:intergenic 40.0%
CCTTGGATACGGATATTGTT+TGG + chr3.2:69829413-69829432 None:intergenic 40.0%
CTGTGGCATATGATAAGCTT+TGG - chr3.2:69831109-69831128 MS.gene25869:CDS 40.0%
CTTTGTAGGTGTATCATGCT+TGG + chr3.2:69829032-69829051 None:intergenic 40.0%
GAAGACCATCATTGGCAAAA+GGG + chr3.2:69831353-69831372 None:intergenic 40.0%
GAAGATCCTAATGCACAACA+TGG - chr3.2:69831303-69831322 MS.gene25869:intron 40.0%
GAAGTTCGAACAAAAGGTCA+CGG - chr3.2:69831477-69831496 MS.gene25869:CDS 40.0%
GACAACTCATTGAACCATCA+TGG - chr3.2:69831826-69831845 MS.gene25869:intron 40.0%
GAGCTCACTAGACCTATTAT+TGG - chr3.2:69830671-69830690 MS.gene25869:CDS 40.0%
GATATGAATGTACTCACCAG+TGG + chr3.2:69830817-69830836 None:intergenic 40.0%
GGGAGATTGAAGGAAATTGA+AGG - chr3.2:69831891-69831910 MS.gene25869:intron 40.0%
TACCAAATGATCTTCTCCAC+CGG - chr3.2:69831151-69831170 MS.gene25869:CDS 40.0%
TCATATACTCGCCAGAATCA+AGG + chr3.2:69830848-69830867 None:intergenic 40.0%
TCCAAGTGCATTTATCTGCA+AGG + chr3.2:69831013-69831032 None:intergenic 40.0%
TCCATCTTAGTTGTCGATCA+TGG - chr3.2:69828999-69829018 MS.gene25869:CDS 40.0%
TCCATGATCGACAACTAAGA+TGG + chr3.2:69829003-69829022 None:intergenic 40.0%
TCCCTCACTTTGCTTACATA+AGG + chr3.2:69830572-69830591 None:intergenic 40.0%
TCTCCCATTCTTCCTTTGTA+GGG + chr3.2:69831686-69831705 None:intergenic 40.0%
TGAAGAATAGGAATGGAGCT+GGG - chr3.2:69831946-69831965 MS.gene25869:CDS 40.0%
TGGACAGAAGTTCGAACAAA+AGG - chr3.2:69831471-69831490 MS.gene25869:CDS 40.0%
TGGTTATACCACAGTTGAAG+AGG - chr3.2:69830411-69830430 MS.gene25869:intron 40.0%
TTACCATCACAAACACCAAG+TGG - chr3.2:69829232-69829251 MS.gene25869:intron 40.0%
TTCTCCCATTCTTCCTTTGT+AGG + chr3.2:69831687-69831706 None:intergenic 40.0%
TTGAAGAATAGGAATGGAGC+TGG - chr3.2:69831945-69831964 MS.gene25869:CDS 40.0%
TTTAGGCCATGTTGTGCATT+AGG + chr3.2:69831312-69831331 None:intergenic 40.0%
! GGATGCAGTAACTTGTAGAT+TGG + chr3.2:69830976-69830995 None:intergenic 40.0%
! GTGCAGGAGACAAATTCTTT+TGG - chr3.2:69830126-69830145 MS.gene25869:intron 40.0%
! TAGTGGTGATGATGTCAATG+AGG + chr3.2:69831551-69831570 None:intergenic 40.0%
! TGAAAGCTTTAGTGACTGTG+AGG - chr3.2:69828432-69828451 MS.gene25869:CDS 40.0%
! TGAGGCTTTTGCTGAGATAA+AGG - chr3.2:69829729-69829748 MS.gene25869:CDS 40.0%
! TGCAAGGTATATCTGAGATG+TGG + chr3.2:69830997-69831016 None:intergenic 40.0%
!! AATCCACTTGGTGTTTGTGA+TGG + chr3.2:69829238-69829257 None:intergenic 40.0%
!! ACCAGCAGATTTTGGAAATG+TGG - chr3.2:69828895-69828914 MS.gene25869:CDS 40.0%
!! ATGAAACCATTTTCAGGACC+TGG - chr3.2:69831987-69832006 MS.gene25869:CDS 40.0%
!! CCAGCAGATTTTGGAAATGT+GGG - chr3.2:69828896-69828915 MS.gene25869:CDS 40.0%
!! GCTTTGGCAATTTGATTTGC+AGG - chr3.2:69831125-69831144 MS.gene25869:CDS 40.0%
!! GGTCGAAAGCATTCTAATAC+AGG - chr3.2:69829445-69829464 MS.gene25869:CDS 40.0%
!! TCACCAGATGAAGAGTACTT+GGG - chr3.2:69831804-69831823 MS.gene25869:intron 40.0%
!! TGCTCACTTTTAAGTGCTTC+TGG + chr3.2:69829997-69830016 None:intergenic 40.0%
!! TTCACCAGATGAAGAGTACT+TGG - chr3.2:69831803-69831822 MS.gene25869:intron 40.0%
!! TTCTTTTAGAGGAATGGCTG+TGG - chr3.2:69831281-69831300 MS.gene25869:intron 40.0%
!!! CAAACACTTGTTTTTGGAGC+TGG - chr3.2:69828505-69828524 MS.gene25869:CDS 40.0%
AAGCCTCCAAAGCCAAAGAT+TGG + chr3.2:69830744-69830763 None:intergenic 45.0%
AGATGCTACCCAAGCTATAG+TGG + chr3.2:69831568-69831587 None:intergenic 45.0%
ATCAAGAGCTTCAAGCATGG+TGG - chr3.2:69831451-69831470 MS.gene25869:CDS 45.0%
CCCACATTTCCAAAATCTGC+TGG + chr3.2:69828899-69828918 None:intergenic 45.0%
CTCAAAAGACCTGTTCTTGG+TGG - chr3.2:69829385-69829404 MS.gene25869:CDS 45.0%
CTGATCAAGAGCTTCAAGCA+TGG - chr3.2:69831448-69831467 MS.gene25869:CDS 45.0%
CTGTTCTTCCTCACCAAACA+AGG - chr3.2:69830626-69830645 MS.gene25869:CDS 45.0%
GATCGACAACTAAGATGGAC+AGG + chr3.2:69828998-69829017 None:intergenic 45.0%
GCGACAAATCAGAAGAACCA+TGG - chr3.2:69831499-69831518 MS.gene25869:CDS 45.0%
GCTTAGTACCTCTTCAACTG+TGG + chr3.2:69830422-69830441 None:intergenic 45.0%
GGATATCATGCACATGTGTG+TGG + chr3.2:69828573-69828592 None:intergenic 45.0%
GTCATCGAGTCGCAGATAAT+TGG - chr3.2:69830391-69830410 MS.gene25869:intron 45.0%
TACCGGTGGAGAAGATCATT+TGG + chr3.2:69831156-69831175 None:intergenic 45.0%
TCCTTTGTAGGGTCTTCTGT+AGG + chr3.2:69831675-69831694 None:intergenic 45.0%
TGTCCCAAGTACTCTTCATC+TGG + chr3.2:69831810-69831829 None:intergenic 45.0%
! CAAGCCTGAGCAAACACTTT+TGG - chr3.2:69831716-69831735 MS.gene25869:intron 45.0%
! CTGGAGTATCAAAGCGCAAA+AGG + chr3.2:69829978-69829997 None:intergenic 45.0%
! GACTACCCTTTTGCCAATGA+TGG - chr3.2:69831345-69831364 MS.gene25869:intron 45.0%
! GTTTTCAGATTTGGCCACCA+TGG + chr3.2:69831519-69831538 None:intergenic 45.0%
! TCTCTCGTGATAGCTGATTC+TGG + chr3.2:69830370-69830389 None:intergenic 45.0%
! TGGCTTCAATGTGCCTTGTT+TGG + chr3.2:69830642-69830661 None:intergenic 45.0%
!! CACTCCAAAAGTGTTTGCTC+AGG + chr3.2:69831723-69831742 None:intergenic 45.0%
!! CTACCAATCTTTGGCTTTGG+AGG - chr3.2:69830738-69830757 MS.gene25869:CDS 45.0%
!! GGAATTTGCTAGGGAGACTT+TGG - chr3.2:69830160-69830179 MS.gene25869:intron 45.0%
!! TCAATGTGCCTTGTTTGGTG+AGG + chr3.2:69830637-69830656 None:intergenic 45.0%
!!! GTTGGGAACTTTTGAAGTGC+AGG - chr3.2:69830110-69830129 MS.gene25869:intron 45.0%
ACAAGGCACATTGAAGCCAC+TGG - chr3.2:69830643-69830662 MS.gene25869:CDS 50.0%
ACTGTGAGGCAAAGTGATGG+TGG - chr3.2:69828446-69828465 MS.gene25869:CDS 50.0%
CTAAGATGGACAGGTCCATC+AGG + chr3.2:69828989-69829008 None:intergenic 50.0%
CTAGTGAGCTCAATGACCAG+TGG + chr3.2:69830662-69830681 None:intergenic 50.0%
GCCTACAGAAGACCCTACAA+AGG - chr3.2:69831671-69831690 MS.gene25869:intron 50.0%
GTACGACAACGCCTTGGATA+CGG + chr3.2:69829424-69829443 None:intergenic 50.0%
GTCCATCAGGAAAACCGTCA+AGG + chr3.2:69828976-69828995 None:intergenic 50.0%
GTGACTCCAGGTCCTGAAAA+TGG + chr3.2:69831996-69832015 None:intergenic 50.0%
GTGACTGTGAGGCAAAGTGA+TGG - chr3.2:69828443-69828462 MS.gene25869:CDS 50.0%
TCTGGATTCTCAGCCCATGA+TGG + chr3.2:69831843-69831862 None:intergenic 50.0%
TGTCTATGTTCCTCGCGATG+AGG - chr3.2:69829711-69829730 MS.gene25869:CDS 50.0%
TTGCTAGGGAGACTTTGGCA+GGG - chr3.2:69830165-69830184 MS.gene25869:intron 50.0%
TTTGCTAGGGAGACTTTGGC+AGG - chr3.2:69830164-69830183 MS.gene25869:intron 50.0%
! CTCGTGATAGCTGATTCTGG+TGG + chr3.2:69830367-69830386 None:intergenic 50.0%
! GTCCTTGACGGTTTTCCTGA+TGG - chr3.2:69828971-69828990 MS.gene25869:CDS 50.0%
! TCAACCTGGGAATGGCCTTT+TGG + chr3.2:69829930-69829949 None:intergenic 50.0%
!! TATCCAAGGCGTTGTCGTAC+CGG - chr3.2:69829424-69829443 MS.gene25869:CDS 50.0%
!! TGGAAATGTGGGCGCTGTTT+TGG - chr3.2:69828907-69828926 MS.gene25869:CDS 50.0%
!! TGTTTGGTGCCACCAAGAAC+AGG + chr3.2:69829397-69829416 None:intergenic 50.0%
!! TTTTCAGGACCTGGAGTCAC+TGG - chr3.2:69831996-69832015 MS.gene25869:CDS 50.0%
ACTCCCTTTCCAGTGACTCC+AGG + chr3.2:69832008-69832027 None:intergenic 55.0%
AGGACCTGGAGTCACTGGAA+AGG - chr3.2:69832001-69832020 MS.gene25869:CDS 55.0%
GGACCTGGAGTCACTGGAAA+GGG - chr3.2:69832002-69832021 MS.gene25869:CDS 55.0%
TCAGCAAAAGCCTCATCGCG+AGG + chr3.2:69829724-69829743 None:intergenic 55.0%
TGCTAGGGAGACTTTGGCAG+GGG - chr3.2:69830166-69830185 MS.gene25869:intron 55.0%
TGCTCCAAAAGGCCATTCCC+AGG - chr3.2:69829923-69829942 MS.gene25869:intron 55.0%
! TTTTACTCCTGAGGACGCCG+TGG + chr3.2:69829773-69829792 None:intergenic 55.0%
AACACAACCACGGCGTCCTC+AGG - chr3.2:69829763-69829782 MS.gene25869:CDS 60.0%
CGACCGGTACGACAACGCCT+TGG + chr3.2:69829430-69829449 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 69828392 69832049 69828392 ID=MS.gene25869
chr3.2 mRNA 69828392 69832049 69828392 ID=MS.gene25869.t1;Parent=MS.gene25869
chr3.2 exon 69831893 69832049 69831893 ID=MS.gene25869.t1.exon1;Parent=MS.gene25869.t1
chr3.2 CDS 69831893 69832049 69831893 ID=cds.MS.gene25869.t1;Parent=MS.gene25869.t1
chr3.2 exon 69831369 69831637 69831369 ID=MS.gene25869.t1.exon2;Parent=MS.gene25869.t1
chr3.2 CDS 69831369 69831637 69831369 ID=cds.MS.gene25869.t1;Parent=MS.gene25869.t1
chr3.2 exon 69830975 69831215 69830975 ID=MS.gene25869.t1.exon3;Parent=MS.gene25869.t1
chr3.2 CDS 69830975 69831215 69830975 ID=cds.MS.gene25869.t1;Parent=MS.gene25869.t1
chr3.2 exon 69830432 69830758 69830432 ID=MS.gene25869.t1.exon4;Parent=MS.gene25869.t1
chr3.2 CDS 69830432 69830758 69830432 ID=cds.MS.gene25869.t1;Parent=MS.gene25869.t1
chr3.2 exon 69830224 69830309 69830224 ID=MS.gene25869.t1.exon5;Parent=MS.gene25869.t1
chr3.2 CDS 69830224 69830309 69830224 ID=cds.MS.gene25869.t1;Parent=MS.gene25869.t1
chr3.2 exon 69830009 69830119 69830009 ID=MS.gene25869.t1.exon6;Parent=MS.gene25869.t1
chr3.2 CDS 69830009 69830119 69830009 ID=cds.MS.gene25869.t1;Parent=MS.gene25869.t1
chr3.2 exon 69829622 69829923 69829622 ID=MS.gene25869.t1.exon7;Parent=MS.gene25869.t1
chr3.2 CDS 69829622 69829923 69829622 ID=cds.MS.gene25869.t1;Parent=MS.gene25869.t1
chr3.2 exon 69829269 69829535 69829269 ID=MS.gene25869.t1.exon8;Parent=MS.gene25869.t1
chr3.2 CDS 69829269 69829535 69829269 ID=cds.MS.gene25869.t1;Parent=MS.gene25869.t1
chr3.2 exon 69828392 69829151 69828392 ID=MS.gene25869.t1.exon9;Parent=MS.gene25869.t1
chr3.2 CDS 69828392 69829151 69828392 ID=cds.MS.gene25869.t1;Parent=MS.gene25869.t1
Gene Sequence

>MS.gene25869

ATGGCAACAAAAAGTAGCACCGAGTCTAAATCTCATAATGTGAAAGCTTTAGTGACTGTGAGGCAAAGTGATGGTGGTCTCATCAAGAATATTGTTACTGGTATTGTTGGTAACAAACACTTGTTTTTGGAGCTGGTTAGTGCTGAACTTGATCCAAGTAAGTTTCATTTTATTGCTTCCACACACATGTGCATGATATCCATTGTCCTTTACAGTAACATCCAAACCATGGTTTATTTATGTTTTAATAACATTTTGTTTATAAAACATGAATATGTATGTACACAAAACTTTGGAGCACCATTCATTGTTACTAGTAAATTGTCAAATCATGTCAAAACTTAAATCGTCACATATGAACACAATTCTTCGAAGGCAACCTAGACCTCACGTTTATTTTATTCAAATGAAGGAACAAATTCGGAGAAAAAGACAATAAAAGGAAAAGCGCACGAAACTGAGAAAAAAGAGAATGAGGTGCAATATGAGGCAACATTTGAGTTACCAGCAGATTTTGGAAATGTGGGCGCTGTTTTGGTTGAGAATGAGCATGACAAAGAGATATTTCTAAATAACATAGTCCTTGACGGTTTTCCTGATGGACCTGTCCATCTTAGTTGTCGATCATGGATTCAACCCAAGCATGATACACCTACAAAGAGAGTTTTCTTCACTAACAAGGTAATAATGATGATCAATTAGTATAATCATTATGTTATATATAAGTTAATTTAAGTTTTATATATACATGGAAGAATTAACCATCCAACTTTATTTACATGACCAAATTTAATTTGTATGCTATTTTCATGTCTGCGTGAAATTAATTAACAGATGTATTTACCATCACAAACACCAAGTGGATTAAGAAAGCTTAGAGAAGATGAGCTAATAGAATTAAGAGGAAATGGAGAAGGAGAAAGAAAAAGCTCAGATAGAATTTATGACTATGATGTCTACAATGATCTTGGAGATCCTGACACCAACATTAAACTCAAAAGACCTGTTCTTGGTGGCACCAAACAATATCCGTATCCAAGGCGTTGTCGTACCGGTCGAAAGCATTCTAATACAGGTTATCTATTCTATTCTAGTTGTCCAGCATATATAAATAATTTTGAACAATTAATTTCTCATGTTCCTTTCTCTAATTTATAACATATATTCTTTTTTCAAATAAAAAAGTATTCTCAAGAAAAAAAGTTATCATATAGTTTGTTCATTCTTTGATTTTGAACATGTAAGCTACCTAGCATATATATATAACACAATTTTATTTTATGTTATATAGATCCATCATATGAAAAGAGAGATTCGGATGTCTATGTTCCTCGCGATGAGGCTTTTGCTGAGATAAAGGAGATACAATTCAACACAACCACGGCGTCCTCAGGAGTAAAAACAATTTTGGAATCCTTGGACACAATTCTCACTGATATAAATCTTGGATTTGACAGCTTTGAAGACATAGACGAACTTTACAAAGAAGGATTTCATTTACCATCATCTCTTGGATCCAATAATTTAACATTGCTCCAAAAGGCCATTCCCAGGTTGATTAAAGTTGCTAATGATAGCCAAAACCTTTTGCGCTTTGATACTCCAGAAGCACTTAAAAGTGAGCACATTTATGATTATATGATAAGTGCTTCTTTTTAAACTTGTTAATTATAATAAACCAAATATTTATTATATTACTTGTTTTACAAGTTATTTGAGTTGGGAACTTTTGAAGTGCAGGAGACAAATTCTTTTGGCTATCAGATGAGGAATTTGCTAGGGAGACTTTGGCAGGGGCCAACCCAAATAGCATCCAATTAGTCAAGGCAATAATTGTTACAAATATTTGGTATTACATTTATAATTATATCATCTATGCTAATTAGGAAATCTTTTGTTCTTTTCTTGGTGTATTCATTATGTTATGTAGGAATGGCCTCTGAGAAGTAAATTAGAATCCCAAATTTATGGTCCACCAGAATCAGCTATCACGAGAGAAGTCATCGAGTCGCAGATAATTGGTTATACCACAGTTGAAGAGGTACTAAGCATCAATGCTCTTATTACATTTAGAATCTTAGATTAAGTTATGGTCAATAATTGTTTAATTTGAATATTATATCCAGGCCATTCAAGAGAAGAAATTGTTTGTACTAGACTACCATGATTTATATCTACCTTATGTAAGCAAAGTGAGGGAACTGAAGAATACTACACTGTATGGATCAAGAACACTGTTCTTCCTCACCAAACAAGGCACATTGAAGCCACTGGTCATTGAGCTCACTAGACCTATTATTGGTGACAAACCACAATGGAAACAAATCTTCACTCCTGCTTCTGATTCTACCAATCTTTGGCTTTGGAGGCTTGCAAAAGCTCATGTTCTTACTCATGATACTGGTTACCATGAACTTATAAGCCACTGGTGAGTACATTCATATCATGTTTTACCTTGATTCTGGCGAGTATATGAAGTAGTGCTAACTTTGTATATGTTGTTGATTTTTACAGGTTAAGGACTCACTGTGTTGTAGAACCATTTGTGATTGCAACAAATAGGCAACTTAGCACCATGCATCCAATCTACAAGTTACTGCATCCACATCTCAGATATACCTTGCAGATAAATGCACTTGGACGTGAGATTCTTATTAGTTCATATGGAGTCATTGAAAGCACATTCTTTACTAAAAAGTATTCAATGGAATTGAGTTCTGTGGCATATGATAAGCTTTGGCAATTTGATTTGCAGGGACTACCAAATGATCTTCTCCACCGGTAAAGTCTATACGTATCATGAATTTCTTCATCACTAATTATAGACGTATTTTACATTTTCCTCCCTAAATTTCTCTCCTATATCAAATTTTCTGATGCAAATTGTTGTTCTTTTAGAGGAATGGCTGTGGAAGATCCTAATGCACAACATGGCCTAAAGCTAGCAATTGAAGACTACCCTTTTGCCAATGATGGTCTTCTCATATGGGATGCAATTAAACAATGGGTGACAGATTATGTCAATCATTACTATCCAAATCCAAGCATCATTGAATCTGATCAAGAGCTTCAAGCATGGTGGACAGAAGTTCGAACAAAAGGTCACGGCGACAAATCAGAAGAACCATGGTGGCCAAATCTGAAAACACAAAAAGACCTCATTGACATCATCACCACTATAGCTTGGGTAGCATCTGCACATCATTCAGCTACAAACTTTGCACAATATGCTTATGGAGGCTATTTTCCTAATAGACCAACAATTGCTAGAAACAACATGCCTACAGAAGACCCTACAAAGGAAGAATGGGAGAAATTTCTGAACAAGCCTGAGCAAACACTTTTGGAGTGTTTCCCTTCACAAATTCAAGCAACCTTAGTGATGACCATTTTGAACTTATTATCATACCATTCACCAGATGAAGAGTACTTGGGACAACTCATTGAACCATCATGGGCTGAGAATCCAGATATAAAGATAGCATTTGAAAGGTTTAATGGGAGATTGAAGGAAATTGAAGGTATAATAGATTCAAGAAATGCAAATACTAATTTGAAGAATAGGAATGGAGCTGGGATAGTACCTTATGAGCTAATGAAACCATTTTCAGGACCTGGAGTCACTGGAAAGGGAGTTCCATATAGCATATCTATTTAG
Protein sequence

>MS.gene25869.t1

MATKSSTESKSHNVKALVTVRQSDGGLIKNIVTGIVGNKHLFLELVSAELDPRTNSEKKTIKGKAHETEKKENEVQYEATFELPADFGNVGAVLVENEHDKEIFLNNIVLDGFPDGPVHLSCRSWIQPKHDTPTKRVFFTNKMYLPSQTPSGLRKLREDELIELRGNGEGERKSSDRIYDYDVYNDLGDPDTNIKLKRPVLGGTKQYPYPRRCRTGRKHSNTDPSYEKRDSDVYVPRDEAFAEIKEIQFNTTTASSGVKTILESLDTILTDINLGFDSFEDIDELYKEGFHLPSSLGSNNLTLLQKAIPRLIKVANDSQNLLRFDTPEALKRDKFFWLSDEEFARETLAGANPNSIQLVKEWPLRSKLESQIYGPPESAITREVIESQIIGYTTVEEAIQEKKLFVLDYHDLYLPYVSKVRELKNTTLYGSRTLFFLTKQGTLKPLVIELTRPIIGDKPQWKQIFTPASDSTNLWLWRLAKAHVLTHDTGYHELISHWLRTHCVVEPFVIATNRQLSTMHPIYKLLHPHLRYTLQINALGREILISSYGVIESTFFTKKYSMELSSVAYDKLWQFDLQGLPNDLLHRGMAVEDPNAQHGLKLAIEDYPFANDGLLIWDAIKQWVTDYVNHYYPNPSIIESDQELQAWWTEVRTKGHGDKSEEPWWPNLKTQKDLIDIITTIAWVASAHHSATNFAQYAYGGYFPNRPTIARNNMPTEDPTKEEWEKFLNKPEQTLLECFPSQIQATLVMTILNLLSYHSPDEEYLGQLIEPSWAENPDIKIAFERFNGRLKEIEGIIDSRNANTNLKNRNGAGIVPYELMKPFSGPGVTGKGVPYSISI