Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26033.t1 | XP_013448450.1 | 80.7 | 109 | 21 | 0 | 1 | 109 | 1 | 109 | 1.00E-39 | 172.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26033.t1 | O03982 | 55.3 | 76 | 33 | 1 | 1 | 75 | 1 | 76 | 1.7e-14 | 80.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26033.t1 | A0A072TYH4 | 80.7 | 109 | 21 | 0 | 1 | 109 | 1 | 109 | 7.5e-40 | 172.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050253 | MS.gene26033 | 0.870607 | 1.28E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26033.t1 | MTR_7g451210 | 80.734 | 109 | 21 | 0 | 1 | 109 | 1 | 109 | 2.41e-61 | 183 |
| MS.gene26033.t1 | MTR_1173s0010 | 76.364 | 110 | 26 | 0 | 1 | 110 | 1 | 110 | 3.26e-59 | 177 |
| MS.gene26033.t1 | MTR_3g434890 | 76.364 | 110 | 26 | 0 | 1 | 110 | 1 | 110 | 3.80e-59 | 177 |
| MS.gene26033.t1 | MTR_3g434960 | 76.364 | 110 | 26 | 0 | 1 | 110 | 1 | 110 | 8.28e-59 | 176 |
| MS.gene26033.t1 | MTR_7g451200 | 76.364 | 110 | 26 | 0 | 1 | 110 | 1 | 110 | 1.51e-58 | 176 |
| MS.gene26033.t1 | MTR_6g049260 | 72.727 | 110 | 30 | 0 | 1 | 110 | 1 | 110 | 1.79e-54 | 165 |
| MS.gene26033.t1 | MTR_3g435000 | 76.768 | 99 | 23 | 0 | 6 | 104 | 10 | 108 | 3.55e-51 | 157 |
| MS.gene26033.t1 | MTR_3g434920 | 72.289 | 83 | 23 | 0 | 28 | 110 | 2 | 84 | 1.03e-40 | 130 |
| MS.gene26033.t1 | MTR_6g049320 | 57.009 | 107 | 44 | 1 | 1 | 105 | 1 | 107 | 4.04e-34 | 114 |
| MS.gene26033.t1 | MTR_6g049280 | 55.140 | 107 | 46 | 1 | 1 | 105 | 1 | 107 | 1.62e-33 | 113 |
| MS.gene26033.t1 | MTR_6g049320 | 56.075 | 107 | 45 | 1 | 1 | 105 | 31 | 137 | 1.72e-33 | 114 |
| MS.gene26033.t1 | MTR_6g049280 | 54.206 | 107 | 47 | 1 | 1 | 105 | 31 | 137 | 8.63e-33 | 112 |
| MS.gene26033.t1 | MTR_1g099800 | 65.672 | 67 | 23 | 0 | 1 | 67 | 1 | 67 | 6.60e-26 | 94.4 |
| MS.gene26033.t1 | MTR_1g099810 | 68.657 | 67 | 21 | 0 | 1 | 67 | 1 | 67 | 9.49e-26 | 94.0 |
| MS.gene26033.t1 | MTR_6g051680 | 59.701 | 67 | 27 | 0 | 1 | 67 | 1 | 67 | 2.29e-25 | 92.0 |
| MS.gene26033.t1 | MTR_6g051690 | 59.701 | 67 | 27 | 0 | 1 | 67 | 1 | 67 | 2.16e-24 | 90.1 |
| MS.gene26033.t1 | MTR_8g098915 | 58.333 | 72 | 30 | 0 | 1 | 72 | 1 | 72 | 1.35e-23 | 88.2 |
| MS.gene26033.t1 | MTR_1g099640 | 43.966 | 116 | 53 | 2 | 1 | 104 | 1 | 116 | 2.57e-23 | 87.4 |
| MS.gene26033.t1 | MTR_1g099815 | 61.194 | 67 | 25 | 1 | 1 | 67 | 1 | 66 | 3.55e-22 | 84.7 |
| MS.gene26033.t1 | MTR_1g102240 | 61.194 | 67 | 25 | 1 | 1 | 67 | 1 | 66 | 6.73e-22 | 84.0 |
| MS.gene26033.t1 | MTR_7g079110 | 56.716 | 67 | 29 | 0 | 1 | 67 | 1 | 67 | 1.85e-20 | 81.3 |
| MS.gene26033.t1 | MTR_0795s0020 | 59.701 | 67 | 21 | 1 | 1 | 67 | 1 | 61 | 8.35e-20 | 78.6 |
| MS.gene26033.t1 | MTR_1g099810 | 66.667 | 48 | 16 | 0 | 20 | 67 | 1 | 48 | 2.10e-16 | 69.3 |
| MS.gene26033.t1 | MTR_8g098915 | 62.264 | 53 | 20 | 0 | 20 | 72 | 1 | 53 | 1.70e-15 | 67.0 |
| MS.gene26033.t1 | MTR_8g028615 | 56.716 | 67 | 29 | 0 | 1 | 67 | 1 | 67 | 7.43e-13 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26033.t1 | AT1G01490 | 57.353 | 68 | 28 | 1 | 1 | 67 | 1 | 68 | 2.57e-20 | 81.3 |
| MS.gene26033.t1 | AT1G01490 | 57.353 | 68 | 28 | 1 | 1 | 67 | 1 | 68 | 2.57e-20 | 81.3 |
| MS.gene26033.t1 | AT1G01490 | 56.061 | 66 | 28 | 1 | 3 | 67 | 30 | 95 | 6.34e-19 | 78.2 |
| MS.gene26033.t1 | AT5G52760 | 37.037 | 108 | 66 | 2 | 2 | 109 | 4 | 109 | 1.86e-14 | 64.7 |
| MS.gene26033.t1 | AT5G52740 | 44.776 | 67 | 35 | 1 | 1 | 67 | 1 | 65 | 4.23e-12 | 58.5 |
| MS.gene26033.t1 | AT5G52750 | 43.939 | 66 | 37 | 0 | 3 | 68 | 5 | 70 | 1.84e-11 | 57.4 |
Find 18 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTGGACACTGCCTTTAC+TGG | 0.181442 | 7.3:-54234151 | None:intergenic |
| AAGACAGCATCTAGCCTTTC+AGG | 0.206805 | 7.3:+54233603 | MS.gene26033:CDS |
| CGAGAAGAAATTGACATTAA+AGG | 0.241272 | 7.3:+54234117 | MS.gene26033:CDS |
| GAGAAGAAATTGACATTAAA+GGG | 0.319300 | 7.3:+54234118 | MS.gene26033:CDS |
| TAGGTTTCAAAGTATTGAAT+TGG | 0.328085 | 7.3:-54234259 | None:intergenic |
| CAGTGTCCAAGCTAAGAAAA+TGG | 0.399021 | 7.3:+54234161 | MS.gene26033:CDS |
| TTAACAGAAAGTAGTGTTGA+AGG | 0.440878 | 7.3:+54233542 | MS.gene26033:intron |
| TTCTGTTGAATCTCTAAAAG+AGG | 0.445440 | 7.3:+54234201 | MS.gene26033:CDS |
| GGGGTCATCTGATAGTAATA+CGG | 0.532976 | 7.3:-54234283 | None:intergenic |
| AATCTCGAAAATATTGTGGT+GGG | 0.543098 | 7.3:-54234303 | None:intergenic |
| ACAGAAAGTAGTGTTGAAGG+TGG | 0.562618 | 7.3:+54233545 | MS.gene26033:intron |
| TAATCTCGAAAATATTGTGG+TGG | 0.564645 | 7.3:-54234304 | None:intergenic |
| AAATTCAATATGTACCTGAA+AGG | 0.592630 | 7.3:-54233617 | None:intergenic |
| ATCTCGAAAATATTGTGGTG+GGG | 0.596272 | 7.3:-54234302 | None:intergenic |
| CATCTGATAGTAATACGGAT+AGG | 0.615334 | 7.3:-54234278 | None:intergenic |
| TAGTAATCTCGAAAATATTG+TGG | 0.619288 | 7.3:-54234307 | None:intergenic |
| AGAAGAAATTGACATTAAAG+GGG | 0.621172 | 7.3:+54234119 | MS.gene26033:CDS |
| GAAGAAATTGACATTAAAGG+GGG | 0.651809 | 7.3:+54234120 | MS.gene26033:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTTTTTATTTTGCAG+GGG | + | chr7.3:54234069-54234088 | MS.gene26033:intron | 15.0% |
| !!! | CATTTTTTTTTTATTTTGCA+GGG | + | chr7.3:54234068-54234087 | MS.gene26033:intron | 15.0% |
| !!! | TCATTTTTTTTTTATTTTGC+AGG | + | chr7.3:54234067-54234086 | MS.gene26033:intron | 15.0% |
| !! | AAAAATAAAATCGATCATGA+TGG | - | chr7.3:54233792-54233811 | None:intergenic | 20.0% |
| !! | AAAGGATATAACAACAAAAA+AGG | - | chr7.3:54233471-54233490 | None:intergenic | 20.0% |
| !! | AAGGATATAACAACAAAAAA+GGG | - | chr7.3:54233470-54233489 | None:intergenic | 20.0% |
| !! | CACAAAAGTCAAATTAAATT+AGG | - | chr7.3:54234013-54234032 | None:intergenic | 20.0% |
| !!! | AATGAATATTTCGTGTTATT+TGG | - | chr7.3:54234052-54234071 | None:intergenic | 20.0% |
| !!! | TATCCTTTCATATTTTAGAA+TGG | + | chr7.3:54233483-54233502 | MS.gene26033:intron | 20.0% |
| ! | AAATTCAATATGTACCTGAA+AGG | - | chr7.3:54233620-54233639 | None:intergenic | 25.0% |
| ! | AAGTTGTGTTCAAAAGTTTA+GGG | + | chr7.3:54233691-54233710 | MS.gene26033:intron | 25.0% |
| ! | AGAAGAAATTGACATTAAAG+GGG | + | chr7.3:54234119-54234138 | MS.gene26033:CDS | 25.0% |
| ! | GAGAAGAAATTGACATTAAA+GGG | + | chr7.3:54234118-54234137 | MS.gene26033:CDS | 25.0% |
| ! | TAAGTTGTGTTCAAAAGTTT+AGG | + | chr7.3:54233690-54233709 | MS.gene26033:intron | 25.0% |
| ! | TAGTAATCTCGAAAATATTG+TGG | - | chr7.3:54234310-54234329 | None:intergenic | 25.0% |
| ! | TGAAGATGATAAAAGATTAC+AGG | - | chr7.3:54233760-54233779 | None:intergenic | 25.0% |
| !!! | CTTCCATTCTAAAATATGAA+AGG | - | chr7.3:54233489-54233508 | None:intergenic | 25.0% |
| !!! | TAGGTTTCAAAGTATTGAAT+TGG | - | chr7.3:54234262-54234281 | None:intergenic | 25.0% |
| AATCTCGAAAATATTGTGGT+GGG | - | chr7.3:54234306-54234325 | None:intergenic | 30.0% | |
| AGTTGTGTTCAAAAGTTTAG+GGG | + | chr7.3:54233692-54233711 | MS.gene26033:intron | 30.0% | |
| CAGGTACATATTGAATTTCA+TGG | + | chr7.3:54233622-54233641 | MS.gene26033:intron | 30.0% | |
| CGAGAAGAAATTGACATTAA+AGG | + | chr7.3:54234117-54234136 | MS.gene26033:CDS | 30.0% | |
| GAAGAAATTGACATTAAAGG+GGG | + | chr7.3:54234120-54234139 | MS.gene26033:CDS | 30.0% | |
| TAATCTCGAAAATATTGTGG+TGG | - | chr7.3:54234307-54234326 | None:intergenic | 30.0% | |
| ! | TTAACAGAAAGTAGTGTTGA+AGG | + | chr7.3:54233542-54233561 | MS.gene26033:intron | 30.0% |
| ! | TTCTGTTGAATCTCTAAAAG+AGG | + | chr7.3:54234201-54234220 | MS.gene26033:CDS | 30.0% |
| ! | TTTAGAATGGAAGTAATTGC+TGG | + | chr7.3:54233496-54233515 | MS.gene26033:intron | 30.0% |
| ACACTTTGCTCAAAACAGAA+AGG | + | chr7.3:54233837-54233856 | MS.gene26033:intron | 35.0% | |
| ATCTCGAAAATATTGTGGTG+GGG | - | chr7.3:54234305-54234324 | None:intergenic | 35.0% | |
| CATCTGATAGTAATACGGAT+AGG | - | chr7.3:54234281-54234300 | None:intergenic | 35.0% | |
| GTTGTGTTCAAAAGTTTAGG+GGG | + | chr7.3:54233693-54233712 | MS.gene26033:intron | 35.0% | |
| TTGTGTTCAAAAGTTTAGGG+GGG | + | chr7.3:54233694-54233713 | MS.gene26033:intron | 35.0% | |
| ! | TTGACACCATTTTCTTAGCT+TGG | - | chr7.3:54234170-54234189 | None:intergenic | 35.0% |
| CAGTGTCCAAGCTAAGAAAA+TGG | + | chr7.3:54234161-54234180 | MS.gene26033:CDS | 40.0% | |
| GGGGTCATCTGATAGTAATA+CGG | - | chr7.3:54234286-54234305 | None:intergenic | 40.0% | |
| ! | ACAGAAAGTAGTGTTGAAGG+TGG | + | chr7.3:54233545-54233564 | MS.gene26033:intron | 40.0% |
| GGGGGATATTGATCCAGTAA+AGG | + | chr7.3:54234138-54234157 | MS.gene26033:CDS | 45.0% | |
| ! | AAGACAGCATCTAGCCTTTC+AGG | + | chr7.3:54233603-54233622 | MS.gene26033:CDS | 45.0% |
| !! | TAACTTTATAAAAAAATATA+AGG | + | chr7.3:54233904-54233923 | MS.gene26033:intron | 5.0% |
| !!! | TTATATTTTTTTATAAAGTT+AGG | - | chr7.3:54233905-54233924 | None:intergenic | 5.0% |
| AGCTTGGACACTGCCTTTAC+TGG | - | chr7.3:54234154-54234173 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 54233375 | 54234339 | 54233375 | ID=MS.gene26033 |
| chr7.3 | mRNA | 54233375 | 54234339 | 54233375 | ID=MS.gene26033.t1;Parent=MS.gene26033 |
| chr7.3 | exon | 54233375 | 54233380 | 54233375 | ID=MS.gene26033.t1.exon1;Parent=MS.gene26033.t1 |
| chr7.3 | CDS | 54233375 | 54233380 | 54233375 | ID=cds.MS.gene26033.t1;Parent=MS.gene26033.t1 |
| chr7.3 | exon | 54233549 | 54233624 | 54233549 | ID=MS.gene26033.t1.exon2;Parent=MS.gene26033.t1 |
| chr7.3 | CDS | 54233549 | 54233624 | 54233549 | ID=cds.MS.gene26033.t1;Parent=MS.gene26033.t1 |
| chr7.3 | exon | 54234089 | 54234339 | 54234089 | ID=MS.gene26033.t1.exon3;Parent=MS.gene26033.t1 |
| chr7.3 | CDS | 54234089 | 54234339 | 54234089 | ID=cds.MS.gene26033.t1;Parent=MS.gene26033.t1 |
| Gene Sequence |
| Protein sequence |
